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Bone repair material and preparation method thereof

A bone repair and microvascular technology, applied in the fields of prosthesis, tissue regeneration, medical science, etc., can solve the problems of complex procedures, time-consuming, difficult application, etc., and achieve the effect of high biocompatibility, simple preparation method, and low cost

Pending Publication Date: 2022-06-24
SUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this prophylactic vascularization approach is difficult to implement in the clinical setting due to the complex and time-consuming in vitro procedure, and therefore, there is still a need to develop new strategies to prevent revascularization

Method used

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  • Bone repair material and preparation method thereof
  • Bone repair material and preparation method thereof
  • Bone repair material and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] The present embodiment relates to a preparation method of ad-MVF / GelMA composite hydrogel, comprising the following steps:

[0041] S11, take 10 mL of adipose tissue, chop it mechanically, wash away the blood and other contaminants on the adipose tissue with 0.9% normal saline, and then add twice the volume (20 mL) of 2 mg / mL type I collagenase solution;

[0042] S12, under humidified atmosphere conditions, digest with rapid stirring at 37 °C for 8 min, and add an equal volume (30 mL) of PBS containing 20% ​​FBS to neutralize type I collagenase;

[0043] S13, filter the suspension through a 500 μm filter screen to remove undigested fat mass on the filter screen, centrifuge the filtrate at 10,000 rpm for 5 min, and remove the fat supernatant;

[0044] S14, filter the suspension through a 30 μm filter screen, filter out the remaining impurity cells, wash off the filtrate on the filter screen with 0.9% normal saline, centrifuge at 10,000 rpm for 5 min, remove the supernata...

Embodiment 2

[0050] The present embodiment relates to a preparation method of ad-MVF / HAMA composite hydrogel, comprising the following steps:

[0051] S11, take 5 mL of adipose tissue, chop it mechanically, wash away the blood and other contaminants on the adipose tissue with 0.9% normal saline, and add 1.5 times the volume of 1.5 mg / mL type I collagenase solution;

[0052] S12, under humidified atmospheric conditions, digest with rapid stirring at 37 °C for 10 min, and add an equal volume of PBS containing 20% ​​FBS to neutralize collagenase type I;

[0053] S13, filter the suspension through a 600 μm filter screen to remove undigested fat mass on the filter screen, centrifuge the filtrate at 12,000 rpm for 3 min, and remove the fat supernatant;

[0054] S14, filter the suspension through a 50 μm filter screen, filter out the remaining impurity cells, wash off the filtrate on the filter screen with 0.9% normal saline, centrifuge at 12000 rpm for 3 min, remove the supernatant, and leave th...

Embodiment 3

[0058] The present embodiment relates to a preparation method of ad-MVF / SilMA composite hydrogel, comprising the following steps:

[0059] S21, take 2.5 mL of adipose tissue, chop it mechanically, wash away the blood and other contaminants on the adipose tissue with 0.9% normal saline, and add three times the volume of 1 mg / mL type I collagenase solution;

[0060] S22, under humidified atmosphere conditions, digest with rapid stirring at 37 °C for 12 min, and add an equal volume of PBS containing 20% ​​FBS to neutralize collagenase type I;

[0061] S23, filter the suspension through a 450 μm filter screen to remove undigested fat mass on the filter screen, centrifuge the filtrate at 800 rpm for 6 min, and remove the fat supernatant;

[0062] S24, filter the suspension through a 45 μm filter screen, filter out the remaining impurity cells, wash off the filtrate on the filter screen with 0.9% normal saline, centrifuge at 800 rpm for 6 min, remove the supernatant, and leave the p...

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Abstract

The invention belongs to the technical field of biomedical materials, and relates to a bone repair material and a preparation method thereof.The bone repair material comprises a microvascular segment and a matrix carrier, the microvascular segment is extracted from adipose tissue, and the matrix carrier is made of a biocompatible material. The bone repair material containing the microvascular tissue is based on the microvessels extracted from the adipose tissue and a biocompatible material matrix, has an intercommunicated macroporous structure and excellent biocompatibility, is short in preparation period and high in plasticity, can obtain base materials with different forms and sizes according to requirements, and is used for simulating a natural tissue structure.

Description

technical field [0001] The invention belongs to the technical field of biomedical materials, and relates to a bone repair material and a preparation method thereof, in particular to a bone repair material containing microvascular tissue. Background technique [0002] In recent years, with the continuous development of bone tissue engineering, various new scaffold materials have provided new options for the treatment of bone defects. The application of bone tissue engineering technology mainly depends on the reconstruction of the vascular network after the implantation of the scaffold material, that is, whether the vascularization can be quickly achieved in the early stage of implantation. Insufficient vascularization can lead to the formation of a fibrous capsule structure between the scaffold material and the surrounding tissue, which restricts the uptake of nutrients and hinders the discharge of metabolic waste, thereby affecting the formation of new bone. Therefore, the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/36A61L27/22A61L27/20A61L27/52A61L27/54A61L27/56
CPCA61L27/3604A61L27/365A61L27/222A61L27/20A61L27/227A61L27/3687A61L27/56A61L27/52A61L27/54A61L2430/02A61L2300/414
Inventor 李泽溪李斌韩凤选肖灿李家颖
Owner SUZHOU UNIV
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