64 results about "Biologic scaffold" patented technology

The invention discloses a magnesium-containing nanoparticle modified biological scaffold composite material as well as a preparation method and application thereof. The magnesium-containing nano particle modified biological scaffold composite material comprises a biological scaffold material and magnesium-containing nano particles modified on the biological scaffold material, the magnesium-containing nano particles comprise nano magnesium oxide particles and / or nano magnesium oxide-polyphenol compound particles, the nano magnesium oxide-polyphenol compound particles are of a shell-core structure, and the shell-core structure is of a hollow structure. Comprising magnesium oxide serving as a core and a polyphenol compound shell layer coating the surface of the magnesium oxide. The magnesium-containing nanoparticle-modified biological scaffold composite material provided by the invention can prolong the degradation and release of magnesium ions, promote improvement of microenvironment problems such as inflammation and active oxygen outbreak after spinal cord injury, regulate and control oxidative stress and inflammatory response, promote nerve regeneration and motor function recovery, and improve the survival rate of spinal cord injury. The problem that nanoparticles stop in a spinal cord injury area is effectively solved, and support is provided for migration, growth and differentiation of endogenous neural stem cells.

The invention discloses a biological scaffold viscoelasticity evaluation method and related equipment, and aims to overcome the defects of an existing biological scaffold viscoelasticity test method and biological tissue suitability quantitative evaluation. The method comprises the following steps: determining a target implantation part, and acquiring force relaxation experimental data of a biological scaffold by using an atomic force microscope; judging a contact point of the probe and the bracket based on the data; determining the moment when the force signal is stable after the support is compressed to the preset deformation according to the contact point as a force relaxation starting point; calculating the characteristic time when the force value is attenuated to half by taking the starting point as a timing starting point as a microscopic viscoelasticity index; and finally, realizing quantitative evaluation of viscoelasticity suitability by comparing the characteristic time of the stent and the target tissue.

The invention provides a nano transdermal sustained-release biological scaffold as well as a preparation method and application thereof, and belongs to the technical field of biomedical materials. The nano transdermal sustained-release biological scaffold provided by the invention comprises the following components: a load and a nano carrier, the load comprises mussel mucin, trehalose and Ectoine; the mass ratio of the mussel mucin to the trehalose to the Ectoine is (4 to 6) to (0.5 to 1.5) to (0.5 to 2.0). Through the innovative design of a quaternary composite system of mussel mucin, trehalose, Ectoine and a nano-carrier, the three technical problems of short slow release period, insufficient targeting property and difficulty in large-scale production in a wound repair material in the prior art are successfully solved.

The invention relates to the technical field of biological scaffolds, in particular to a silk fibroin-based biological 3D intelligent response scaffold and a preparation method and application thereof.The silk fibroin-based biological 3D intelligent response scaffold adopts a three-layer bionic gradient structure of an oriented fiber membrane, a porous sponge layer and an interface layer, simulates natural tissues, and has the advantages that the bionic gradient structure is improved; mechanical guidance, cell inhabitation and biological activity interfaces are provided respectively, and high-quality regeneration of blood vessels, nerves and skin is promoted synergistically. According to the present invention, the pH / enzyme / temperature triple response unit is provided, such that the lesion microenvironment can be perceived, the targeted release can be triggered, the problem of high burst release rate of the traditional physical drug loading can be solved, and the on-demand accurate drug delivery can be achieved. The SF / HPMC gel is adopted for 3D printing, the printing precision is high, and the porosity difference is 1t; the product performance is stable, and the pore structure can be precisely designed and customized according to the regeneration requirements of different tissues (such as blood vessels, nerves and skin).

The invention relates to the technical field of food processing, in particular to a high-yield nutrient 3D printing biological scaffold prepared on the basis of neurospora crassa spores and bean dregs. The biological scaffold prepared by the invention can form a closed-loop system of agricultural wastes, biological carriers and high-value products. The system has the characteristics of high efficiency, sustainability, flexibility, controllability and the like and the capabilities of modular design and dynamic regulation and control, programmable regulation and control of active compound synthesis can be realized through material-cell interaction, and a novel manufacturing mode is provided for synthesis and precise biosynthesis of nutrient substances such as fungal protein and carotenoid.

The present disclosure relates to compositions, apparatus and methods for generating one or more scaffolds, including: mixing a hydrogel material and / or an extracellular matrix (ECM) protein in an aqueous solvent to generate an aqueous process solution; and cryoelectrospinning the aqueous process solution onto a plurality of conductive probes extending from a conductive surface of a collector plate disposed within a process chamber under conditions sufficient to generate one or more scaffolds configured to mimic a preselected soft tissue decellularized extracellular matrix. Scaffold compositions are also provided having preselected or tuned characteristics.

The invention provides a porous biological scaffold and a preparation method thereof, and the porous biological scaffold is prepared by pre-freezing and freeze-drying a mixed solution formed by a hydrophobic polyester material and a hydrophilic polymer material, the stent has a three-dimensional porous structure, can be compressed to be smaller than the original volume in a dry state, and can recover to the original volume after being released from a compressed state. According to the invention, the hydrophobic polyester material and the hydrophilic polymer material cooperate with each other, so that the material has unique dynamic response performance, specifically, the material has good compressibility in a dry state, can be compressed to be smaller than the original volume so as to be conveyed into a body, and can be recovered to the original volume before compression after being implanted into the body; and plugging, filling or mechanical supporting functions are realized.

The invention relates to the technical field of biology, and particularly discloses a recombinant humanized II-type collagen biological scaffold and application thereof in cartilage repair. The recombinant humanized II-type collagen biological scaffold is formed by freezing and drying a polypeptide solution, and the amino acid sequence of polypeptide comprises n repetitive units, the repetitive unit comprises any one of the following amino acid sequences (1)-(3) or a variant sequence thereof: (1) an amino acid sequence as shown in SEQ ID NO.1; (2) an amino acid sequence having at least 50%-99% sequence identity with the amino acid sequence as shown in SEQ ID NO.1; (3) a variant sequence obtained by performing mutation of one or more amino acid residues on the amino acid sequence as shown in SEQ ID NO.1; wherein n is an integer greater than or equal to 1. The biological scaffold disclosed by the invention does not contain a cross-linking agent and an organic solvent, has high biocompatibility and can quickly repair cartilage tissues.

Encasement structures and methods of customizing patient drug delivery profiles using an encasement structure are described herein. Encasement structures can be configured to receive an implantable medical device and physicians can implant the medical devices within the encasement structures. Encasement structures can include at least one sheet of a bioscaffold material and one or more depots. Depots can be configured to release an active agent, such as an antibiotic, to the medical device within the encasement structure and / or the surrounding tissue. The depots can be insertable into or integrated with the at least one sheet of bioscaffold material.

The application belongs to the technical field of 3D printing biomaterials, and particularly relates to a collagen biomaterial ink for in-situ real-time 3D printing by one-step method as well as a preparation method and application thereof. The collagen biomaterial ink is prepared by mixing a collagen solution, methacrylic anhydride and a photoinitiator, and is cured under irradiation of blue light with a wavelength of 405 nm; the collagen biomaterial ink has good extrudability, and can be 3D printed to obtain a fine micron-level structure; the biological scaffold printed by the collagen biomaterial ink has good biocompatibility and bioactivity, and can significantly promote the repair of skin damage.

The invention discloses a preparation method of a vascular organ chip based on a degradable bionic stent. The degradable biological scaffold (PCL-FBE scaffold for short) is obtained by mixing a swimming bladder extract and polycaprolactone and carrying out electrostatic spinning. On the basis, a vascular organ chip which can be integrated in a micro-fluidic system is further constructed, and a whole process for completing three-dimensional dynamic culture of vascular organs by using the chip is established. According to the present invention, the swim bladder extract and polycaprolactone are compounded to form the electrospinning scaffold, such that the defects of the mechanical property, the degradability and the biocompatibility of the traditional bionic scaffold are successfully overcome, the organoid culture effect is significantly improved, and the efficient and sustainable innovation platform is provided for the tissue engineering and the regenerative medicine.

A method of modifying a surface of a three-dimensional (3D) article includes immersing at least a portion of the 3D article in a buffer solution of a functionalized peptide such that the functionalized peptide reacts with a reactive group on the surface of the 3D article; and washing at least a portion of the 3D article to remove unreacted functionalized peptides. The surface-modified 3D article comprises a plurality of peptides covalently bonded to a surface of the 3D article by cysteine bridging. The surface-modified 3D articles can be used as scaffolds for constructing biological tissues or implants.

The present application relates to a bioprinting system and method for precise cell positioning, and relates to the field of three-dimensional printing technology. The system includes: a three-dimensional motion module, which is used to achieve precise movement of the print head; a printing module, which includes a print head, which includes a printing nozzle, and the printing module is used to construct a biological scaffold and / or eject cells; an information acquisition module, which includes a camera for real-time positioning of the physical position and / or cell position of the biological scaffold; a software module, which is used to achieve motion control of the print head. The bioprinting system and method for precise cell positioning of the present application achieves precise cell positioning by precisely printing a single cell or a single row of cell sequences onto a target biological scaffold, providing the necessary foundation for the subsequent orderly growth, proliferation and signal transduction of cells.

The invention provides a visual biological scaffold capable of discharging percolate in a self-pumping manner and a preparation method of the visual biological scaffold. The visual biological scaffold comprises the following components in parts by weight: 5-20 parts of a hydrophobic high polymer material, 80-95 parts of a volatile solvent, 5-10 parts of a hydrophobic component, 3-15 parts of a hydrophilic high polymer material and 0.1-15 parts of an antibacterial agent. A hydrophilic and hydrophobic material is co-spun through electrostatic spinning, the material is endowed with certain hydrophilicity, the attachment and proliferation rate of cells can be increased, wound healing is promoted, a 3D network microchannel is formed, and the channel has a self-pumping drainage effect and can be used as a miniature infusion pump; the antibacterial substance is carried through electrostatic spinning, the antibacterial substance is physically wrapped in the fiber or dispersed in a fiber matrix or adsorbed on the surface of the fiber, and the antibacterial substance has a slow release effect, so that efficient and continuous sterilization is achieved, and wound infection is prevented; the device has the characteristic of visualization, and the wound healing degree can be observed; the electrostatic spinning in-situ forming technology is adopted, and the biological scaffold deposited on the wound has adhesiveness and does not need to be fixed additionally.

The invention belongs to the technical field of biomedical materials, and discloses a bone tissue repair material for promoting vascularization and new bone generation and a preparation method thereof. The bone tissue repair material is composed of a decalcified bone matrix and a degradable biological source gel material. The repair material has a loose porous three-dimensional structure similar to that of natural cancellous bone, and the temperature-sensitive gel material filled in the pore structure of the repair material can form gel in situ in vivo and is attached to pores of a decalcified bone matrix, so that cell migration, adhesion and proliferation can be promoted, the degradation rate of gel in vivo can be reduced, and the repair material has a good application prospect. The continuous release of the pro-angiogenesis factor is ensured. Good biocompatibility is achieved, and cytotoxicity and immunogenicity are avoided; meanwhile, in the slow degradation process, angiogenesis promoting factors are continuously released for a long time, and angiogenesis and new bone generation are promoted. Therefore, the implanted biological scaffold prepared from the bone tissue repair material has excellent bone repair and regeneration promotion capability and wide application prospect.

The invention provides a small-caliber blood vessel biological scaffold capable of adjusting stress relaxation and a preparation method of the small-caliber blood vessel biological scaffold. The biological scaffold is of a tubular structure which is formed by arranging micron fibers and nanofibers in a crossed mode, is arranged in a net shape and is provided with a layered tube wall. The small-caliber blood vessel biological scaffold has adjustable stress relaxation performance, biodegradability, bionic viscoelasticity and excellent surgical suture performance, endogenous cells can be guided to be clustered and self-assembled, a small-caliber blood vessel graft with the deformation behavior close to that of a natural blood vessel is formed, and the small-caliber blood vessel biological scaffold has the advantages of being simple in structure and convenient to use. And the double bottleneck that the mechanical property and the tissue regeneration function of the traditional stent are difficult to consider is solved.

This invention relates to a decellularized oral collagen plug and its preparation method. The plug comprises an upper decellularized matrix membrane and a lower decellularized sponge plug. The preparation method is as follows: pretreated fresh animal-derived membrane material is treated with trypsin solution and sodium hydroxide solution, washed with purified water to obtain a decellularized matrix membrane; a portion of the obtained decellularized matrix membrane is placed in a low-temperature tissue ball mill, pulverized, homogenized, filtered, and washed with purified water to obtain a decellularized collagen solution; the decellularized collagen solution is poured into a freeze-drying mold, defoamed under low-temperature vacuum, covered with the decellularized matrix membrane, and after vacuum defoaming, freeze-dried and vacuum thermally cross-linked to obtain the finished collagen plug. This invention's decellularized oral collagen plug is suitable for filling and covering wounds after tooth extraction. The upper decellularized matrix membrane effectively prevents fibroblasts and epithelial cells from invading bone defect areas, while the lower decellularized sponge plug stabilizes blood clots, provides a biological scaffold, reduces postoperative complications, and promotes wound healing.

The growth factor profile, connective tissue matrix constituents, and immunoprivileged status of urodele extracellular matrix (ECM) and accompanying cutaneous tissue, plus the presence of antimicrobial peptides there, render urodele-derived tissue an ideal source for biological scaffolds for xenotransplantation. In particular, a biological scaffold biomaterial can be obtained by a process that entails (A) obtaining a tissue sample from a urodele, where the tissue comprises ECM, inclusive of the basement membrane, and (B) subjecting the tissue sample to a decellularization process that maintains the structural and functional integrity of the extracellular matrix, by virtue of retaining its fibrous and on-fibrous proteins, glycoaminoglycans (GAGs) and proteoglycans, while removing sufficient cellular components of the sample to reduce or eliminate antigenicity and immunogenicity for xenograft purposes. The resultant urodele-derived biomaterial can be used to enhance restoration of skin homeostasis, to reduce the severity, durations and associated damage caused by post-surgical inflammation, and to promote progression of natural healing and regeneration processes. In addition, the biomaterial promotes the formation of remodeled tissue that is comparable in quality, function, and compliance to undamaged human tissue.

The invention relates to the field of bone repair, and discloses a 3D printing bone-imitating biological scaffold and a preparation method thereof.The preparation method comprises the steps that firstly, ammonium bromide is added into a mixture of deionized water and absolute ethyl alcohol, and an ammonium bromide solution is obtained; sequentially adding an ammonia solution, tetraethoxysilane, triethyl phosphate, calcium nitrate and sodium vanadate, continuously stirring, centrifuging, and collecting a precipitate; washing, aging, heating, cooling, grinding, crushing, and screening to obtain V-MBG powder; preparing a TA solution and a V-MBG solution, mixing and putting the TA solution and the V-MBG solution, and freeze-drying to obtain TA-coated V-MBG powder; preparing a trichloromethane solution of PLGA (poly (lactic-co-glycolic acid)), adding the V-MBG powder, fully stirring, and pumping out the trichloromethane solution to obtain solid slurry; printing an inner-layer stent and a hollow-tube outer-layer stent to obtain a bone-imitating biological stent, and soaking the bone-imitating biological stent in a TA solution to obtain a PLGA / TA-V-MBG composite stent; the problems that in the prior art, the bone repairing effect is poor, the bone-like cell proliferation and differentiation effect is poor, and bone defect repairing caused by bone tumor treatment cannot be achieved are solved.

The present invention relates to the fields of biomaterials and food, and particularly to a method for physically crosslinking to prepare a cell-cultured meat biocompatible scaffold. The physical crosslinking method enables the internal combination of the biocompatible scaffold material through physical forces to form a biocompatible scaffold. The physical crosslinking method comprises the following steps: 1) shaping and drying the scaffold material; 2) steam crosslinking the dried scaffold material. The present invention uses a physical crosslinking method to prepare an edible protein or polysaccharide scaffold to support animal cell culture. No chemical crosslinking reagents are used during the production process, and no coatings such as ECM proteins are used. At the same time, cells proliferate well on the scaffold. The physical crosslinking step of this method can be combined with the sterilization step, with simple operating steps, low cost, and is applicable to the preparation of scaffolds of various forms.

The invention belongs to the technical field of virus inactivation equipment, and particularly relates to a virus inactivation device for artificial biological tendon preparation, which comprises a normal saline cylinder, the lower surface of the normal saline cylinder is fixedly connected with a plurality of support legs, and the outer walls of the plurality of support legs are jointly and fixedly connected with a bottom plate; the lower surface of the bottom plate is fixedly connected with a driving motor and a PLC. The virus inactivation device for preparing the artificial biological tendon has the functions of virus separation and inactivation, provenance cells and a biological scaffold material are prevented from being damaged in the virus inactivation process, the activity and the function of the provenance cells are guaranteed, and the adverse effect of the performance of the biological scaffold material is avoided; and the device has an efficient protection function through the heat preservation mechanism, so that the preparation quality of the artificial biological tendon is effectively improved, meanwhile, no accurate control step and no complex instrument are used in the virus inactivation process, time and labor are saved, and the preparation convenience and efficiency of the artificial biological tendon are effectively improved.

The invention belongs to the technical field of cell culture devices, and particularly relates to a device for in-vitro construction of a 3D neural immune cell co-culture model.The device comprises a vessel body container, a vessel cover is arranged outside the vessel body container, a biological scaffold is arranged in the vessel body container, and the biological scaffold comprises a static column, a plurality of movable plates and a plurality of half grooves; the plurality of movable plates are arranged around the static column and can rotate by taking the static column as an axis, the plurality of movable plates divide the vessel body container into a plurality of culture chambers, and each movable plate is provided with a plurality of gaps, so that the plurality of culture chambers are communicated through the gaps; half grooves are formed in the opposite positions of every two adjacent movable plates; and when two adjacent movable plates are folded, the corresponding half grooves are spliced to form a complete cell inoculation groove. The device is good in stability, different culture chambers are arranged in the vessel body container, materials cannot be leaked to the outside of the vessel body container, pollution is prevented, and safety is high.

The application discloses application of a Histatin-1 polypeptide in preparation of a preparation for promoting sciatic nerve regeneration or repair, and an amino acid sequence of the Histatin-1 polypeptide is shown as SEQ ID NO. 1. The Histatin-1 polypeptide can promote expression of a nerve differentiation index, promote recovery of a functional index after sciatic nerve injury, and promote recovery of a histological index after sciatic nerve injury, for example, recovery of myelin structure and morphology. In addition, in combination with gelatin methacrylate (Gel-MA) as a biological scaffold, slow release of the Histatin-1 polypeptide can be realized, continuous repair support can be provided for a nerve injury site, myelin repair effect is further optimized, a more efficient and more durable solution is provided for treatment of sciatic nerve injury, and the application has a wide application prospect in the field of treatment of myelinated nerve injury.

The present application belongs to the technical field of biological scaffolds, and particularly relates to a preparation method of a porous biomimetic bone scaffold and the biomimetic bone scaffold. The preparation method of the porous biomimetic bone scaffold comprises the following steps: mixing a hydrogel main material, water, a crosslinking agent, an alkyl amine compound and an optional complex high polymer material; and performing directional freezing treatment on the obtained mixed product, wherein the hydrogel main material is selected from one or more than two of silk fibroin, collagen and gelatin, and the complex high polymer material is selected from one or more than two of hyaluronic acid, chitosan and other biological source natural polymer and synthetic organic high polymer compound. The porous biomimetic bone scaffold with certain pore size directional micropore channels and nanochannels formed along the side walls of the directional micropore channels can be prepared by the method.

The invention discloses a 3D printing rapid curing method suitable for a hydrogen-bond-rich polymer and a biological scaffold of the hydrogen-bond-rich polymer. The 3D printing rapid curing method comprises the following steps: step 01, preparing direct-writing printing ink containing the hydrogen-bond-rich polymer and a solvent; 02, the ink is used for constructing a preset structure through direct-writing type three-dimensional printing; and step 03, placing the predetermined structure constructed in the step 02 in a substitute solvent which is mutually soluble with the solvent but does not dissolve the polymer to realize polymer precipitation and solidification. After printing is completed, a predetermined structure is immediately soaked in a high-polarity alternative solvent (such as water), and an original printing solvent is rapidly released by utilizing a hydrogen bond reconfiguration principle, so that rapid precipitation and forming of a polymer are promoted. The nano-particles provided by the invention can promote solvent diffusion: the hydroxyapatite nano-particles are introduced into the ink to construct an internal micro-turbulence and diffusion channel, so that the solvent evaporation rate is remarkably improved, and meanwhile, the strength, the biological activity and the osteogenesis performance of the scaffold are improved.

The application relates to a preparation method of a bacterial cellulose-based multilayer structure functional bionic scaffold, which comprises the following steps: firstly, injecting a biomaterial solution into a silica gel model with a microchannel system to perform freeze drying to obtain a porous biological scaffold; secondly, performing crosslinking treatment on the porous biological scaffold; thirdly, inoculating a culture bacteria solution with a Xylobacter concentration of 10 9 ~ 10 15 individuals / mL on the crosslinking-treated porous biological scaffold, and performing static culture and freeze drying to obtain a bacterial cellulose-based composite scaffold; and finally, adopting a coating solution to coat the bacterial cellulose-based composite scaffold through a microchannel system, and performing demolding treatment to obtain the bacterial cellulose-based multilayer structure functional bionic scaffold. The application provides a functional coating process for the multilayer bionic scaffold by using the shrinkage space of the treated material, the micro-pore channel and the injection method of the patterned drainage network, effectively prevents the penetration of the coating solution, and realizes the perfect solidification of the coating solution on the scaffold.

The invention discloses a biological scaffold material as well as a preparation method and application thereof. The biological scaffold material comprises a composite scaffold inner layer and an outer layer, wherein the composite scaffold inner layer is composed of polycaprolactone and chitosan, and the outer layer is composed of a polydopamine coating carrying nerve growth factors. The preparation method comprises the following steps: firstly, preparing a composite scaffold serving as an inner layer from polycaprolactone and chitosan, then synthesizing a polydopamine solution, adding nerve growth factors, and finally immersing the composite scaffold into the polydopamine solution added with the nerve growth factors to obtain the biological scaffold material. The biological scaffold material can be used as a medical scaffold material for treating spinal cord injury.

A three dimensional biological scaffold structure that includes a biologically compatible material that forms walls of multiple pores. The pores have a range of pore sizes including a pore size of less than 50 μm and a pore size of greater than 100 μm and also including pores having one or more intermediate pore sizes between 50 μm and 100 μm. The pores occupy more than 50% of a volume of the scaffold structure.

The invention discloses a biological scaffold energy storage and dissipation modulus analysis method and related equipment, and aims to overcome the defect that in the prior art, key viscoelastic mechanical characteristics of a biological scaffold and target spinal cord tissue of the biological scaffold under micro-scale and dynamic conditions cannot be accurately and comprehensively represented and analyzed. The method comprises the following steps: acquiring force-time curve data at a micro-scale, and aligning a time zero point to a relaxation starting point to obtain regular sequence data; fourier transform is carried out on the regular data to obtain complex shear moduli, wherein the complex shear moduli respectively comprise an energy storage modulus and a dissipation modulus; and generating a frequency spectrum curve based on the complex shear modulus and obtaining a modulus curve. The comprehensive characterization of the wide-frequency-domain dynamic viscoelasticity of the biological scaffold under the microscopic scale is realized, and accurate data support is provided for mechanical property optimization of the scaffold.