174 results about "Biologic scaffold" patented technology

The invention discloses a double-layer membranous tissue repair material and a preparation method thereof, wherein, a cell-free membranous biological derivative material is used as a surface layer, and a fibroblast is compounded in the interior of a biological support material to form a substrate, and then the surface layer and the substrate are combined in a chimeric way to form the double-layer membranous tissue repair material; a compact surface layer structure can effectively reduce the loss of water, electrolytes and protein from surface of wound, avoid the invading and the reproduction of bacteria to the impaired surface of wound as well as prevent the infection of the surface of wound, thus being beneficial to epitheliosis and epithelial growth; the substrate can directly repair the surface of wound, promote the ingrowth of cells around the surface of wound and the angiogenesis, induce the differentiation from stem cells to skin cells and quicken wound healing; compared with the existing products, the tissue repair material has the advantages of being capable of promoting the regeneration of skin, improving the elasticity, the flexibility and the mechanical abrasion resistance of skin after the surface of wound is healed, reducing hyperplasia of scar tissues, controlling the contracture, having excellent biocompatibility, increasing the success rate of transplant and improving the quality of healing; the invention has wide material resources and simple production method; the double-layer membranous tissue repair material prepared is applicable to the clinical treatment of skin defect caused by inflammation, ulcer, thermal burns, iatrogenicity and the like.

The invention provides dialdehyde carboxymethyl cellulose-collagen frozen gel and a preparation method thereof. The method comprises the following steps of: first uniformly mixing 1 to 3 mass percent of the aqueous solution of collagen and 0.01 to 1 mass percent of the aqueous solution of dialdehyde carboxymethyl cellulose; then injecting the mixed solution into a mold and storing the mixed solution in a low-temperature reactor at the temperature of -40 to 0 DEG C for 1 to 7 days; and finally, taking the obtained product out and slowly unfreezing the obtained product to obtain the dialdehyde carboxymethyl cellulose-collagen frozen gel. The dialdehyde carboxymethyl cellulose-collagen freezing gel prepared by the method is improved in mechanical property, thermal stability and enzymatic degradation resistance at the same time of maintaining the biological activity of collagen gel per se, has the advantages of porosity, hydrophilicity, water absorbing and preserving property, good market application prospect and the like, and is widely used in biomedical fields, such as biologic scaffolds, cell culture, drug delivery, tissue engineering, wound and burning treatment and the like.

The invention discloses a double-layer artificial skin and a preparation method thereof, wherein, cell-free membranous biological derivative material is used as a surface layer, and a fibroblast, extracellular matrix synthesized and secreted by the fibroblast and a cell growth factor are compounded in the interior of biological support material to form a dermis, and then the surface layer and the dermis are combined to form the double-layer artificial skin; a compact surface layer structure can effectively reduce the loss of water, electrolytes and protein from surface of wound, avoid the invading and the reproduction of bacteria to the impaired surface of wound as well as the infection of the surface of wound, and be beneficial to epitheliosis and epithelial growth; the dermis can directly repair the surface of wound, promote the ingrowth of cells around the surface of wound and the angiogenesis, induce the differentiation from stem cells to skin cells and quicken wound healing; the artificial skin has the advantages of being capable of promoting the regeneration of skin, improving the elasticity, the flexibility and the mechanical abrasion resistance of skin after the surface of wound is healed, reducing excess scar tissues, controlling the contracture, having excellent biocompatibility, increasing the success rate of transplant and improving the quality of healing; the double-layer artificial skin has wide material resources and simple production method, and is applicable to the clinical treatment of skin defect caused by inflammation, ulcer, thermal burns, iatrogenicity and the like.

The invention provides an assembling type cell-derived extracellular matrix membrane (ECM) compound bone repairing material as well as a preparation method and application thereof. The bone repairingmaterial is prepared by the following steps: completely covering a three-dimensional degradable biological scaffold with a sheet-shaped acellular matrix membrane under sterile conditions and then freezing the three-dimensional degradable biological scaffold for 24 to 48h under the condition of -20 to -80 DEG C; then carrying out freeze drying treatment to obtain the ECM membrane compound bone repairing material, wherein the sheet-shaped acellular matrix membrane is a cell-derived extracellular matrix which is generated by in-vitro culture of cells, is subjected to decellularization treatment and has certain bone induction activity, and the three-dimensional degradable biological scaffold is a biological scaffold which is prepared from natural polymers, has a three-dimensional porous microscopic structure and can be completely degraded and absorbed in human bodies. The assembling type cell-derived extracellular matrix membrane compound bone repairing material has a good bone induction capability; furthermore, the bone regeneration capability of the compound material is strengthened; a construction method is simple and feasible and the material can be stored for a long time and has awide clinical application potential.

A tissue-engineered epidermis is prepared from skin fibroblasts through obtaining the skin fibroblasts, preparing culture medium, amplifying culture, preparing extocytic matrix compound, preparing biologic scaffold, compounding the skin fibroblasts on the surface of biologic scaffold, and 3D culture. Its advantages are a certain elasticity and toughness, short culture time, and no obvious immunorejection reaction.

The invention discloses a preparation method of an autologous platelet-factor-rich plasma (PFRP) preparation. The preparation method is characterized by comprising the steps of: A, preparation of autologous PFRP, to be specific, collecting autologous whole blood, and preparing the autologous PFRP in a centrifuging and freeze-thawing manner; and B, preparation of the PFRP preparation, to be specific, mixing the obtained PFRP with a biological scaffold/autologous somatic cells according to a volume ratio of (3:1)-(1:1) to obtain the PFRP preparation. By means of specially processing the autologous whole blood to obtain the autologous PFRP, pollution risks caused by adding an activating agent are reduced, and the immune risks of a membrane antigen during heterogenous usage are avoided. The autologous PFRP preparation prepared according to the method not only has a good effect of repairing damaged tissues such as meniscus, but also has very good anti-wrinkle, filling and lifting effects.

The invention provides an organic composite hydrogel cross-linked by a modified gelatin nano-microsphere and a preparation method of the organic composite hydrogel. Specifically, the preparation method comprises the following steps: preparing a modified gelatin nano-microsphere by virtue of a two-step solvent removal process, then respectively dissolving an acrylamide monomer and the modified gelatin nano-microsphere in de-ionized water and stirring in an inert atmosphere for 5-30min; adding a catalyst, stirring in the inert atmosphere for 5-10min, and adding an initiator; carrying out a free radical polymerization reaction at minus 40-40 DEG C for 12-48hr; and soaking a reactant in de-ionized water at 20 DEG C, replacing the de-ionized water once every other 5-8hr, and keeping for 48-72hr to obtain the organic composite hydrogel. The method disclosed by the invention is simple in process and controllable in condition, and the prepared organic composite hydrogel integrates the characteristics of a nano composite material and covalent cross-linking and has the characteristics of being good in biocompatibility, mechanical property, macro-porous property, rapid swelling property and the like; and the organic composite hydrogel can be widely applied to the biomedical materials of biological scaffold, cell culture, medicine controlled release, tissue engineering and the like.

The invention provides an exosome targeted slow-release microsphere biological stent and a preparation method and a use thereof; the biological stent includes collagen, degradable microspheres dispersed in the collagen, and an exosome combined on the microspheres. The preparation method comprises the steps: separating collagen from animal tissues; separating an exosome from a culture substance ofbone marrow or umbilical cord mesenchymal stem cells; preparing microspheres combined with the exosome by an emulsification-crosslinking method; and mixing the microspheres with the collagen, and freeze-drying. Chitosan and other high molecular materials are combined with the exosome by a microsphere structure, the microsphere structure is dispersed to the collagen, a biological stent material having a function of directional release of the exosome is formed, the exosome targeted repairing and treatment effects can be significantly improved, and the microsphere structure makes the release of the exosome have slow-release controllable characteristics, accords with development trend of accurate drug diversity, has diverse dosage forms, and has broad clinical application value.

The invention discloses a mesenchymal stem cell biological winkle removing agent and a preparation method thereof. The winkle removing agent is prepared by uniformly mixing mesenchymal stem cells, recipient blood plasma, growth factors, insulin and hyaluronic acid. The preparation method comprises the following steps of: (1) collecting and culturing the mesenchymal stem cells to prepare cell concentrated solution; (2) collecting the recipient blood plasma; (3) adding the growth factors and the insulin into the recipient blood plasma; and (4) using the hyaluronic acid as a biologic scaffold, and repeatedly sucking and uniformly mixing 2 percent hyaluronic acid, the blood plasma into which the growth factors and the insulin are added and the mesenchymal stem cell concentrated solution in aninjector to obtain a mesenchymal stem cell preparation. The winkle removing agent prepared by the method is used for removing and preventing skin winkles and has the advantages of quick response, long maintenance time, non-stiff facial expression, unobvious hardening of hyperplastic tissues and skin texture accordant with normal skin.

The invention provides a collagen-based biomedical material by taking dialdehyde polyethylene glycol as a cross-linking agent and a preparation method thereof. The preparation method disclosed by the invention comprises the following steps of: preparing dialdehyde polyethylene glycol having a certain hydroformylation degree at first, uniformly mixing dialdehyde polyethylene glycol solution with the mass fraction of 0.1-5% with collagen solution with the mass fraction of 0.3-3%, then, pouring into a mould, freezing at -30-0 DEG C for 1-10 days, taking out, and freeze-drying in a freeze-drying machine for 1-2 days to obtain dialdehyde polyethylene glycol-collagen sponge; and repetitively immersing the sponge in water for 2-4 times, each time for 2-10 h to obtain dialdehyde polyethylene glycol-collagen hydrogel. According to the invention, a freezing polymerizing technology and a freeze drying technology are combined; the mechanical property, the thermal stability and the enzymatic degradation resistant property of collagen are improved by the prepared dialdehyde polyethylene glycol collagen sponge or gel; furthermore, the collagen-based biomedical material disclosed by the invention has the advantages of porosity, hydrophilia, water-retaining property, nontoxicity, good biocompatibility and the like, can be used in the biomedical fields, such as biological scaffolds, cell culture, drug release, burn and wound dressing and the like, and has good market application prospect.

A bioscaffold and method of manufacture is described. The bioscaffold comprised greater than 80% type I collagen fibers or bundles having a knitted structure providing tensile load strength. The method of manufacture comprises the steps of: (a) isolating collagen fibers or bundles; (b) incubating said fibers or bundles in a mixture of NaOH, alcohol, acetone, HC; and ascorbic acid; and (c) mechanical manipulation of said fibers or bundles to produce a knitted structure.

The invention provides a method for preparing a biological scaffold by 3D printing. The method comprises the steps of grinding allogeneic bones or heterogeneous bones at a low temperature in a freezing grinder to obtain a printing material, modeling 3D printing with the aid of a computer, and then performing post treatment in a genipin solution. The prepared biological scaffold has good biological compatibility, and the bone graft material does not have toxicity, rejection, mutagenicity or antigenicity in vivo, and does not disturb bone and tissue regeneration; the prepared biological scaffold can be gradually degraded and absorbed and replaced by autologous bone tissues, can bear the pressure close to 20MPa with normal bone cortex, and has good initial mechanical properties; and the elastic modulus is gradually reduced, stress shielding is avoided, fracture, collapse and loosening of implants in the long-term healing process are avoided, bone fusion can be accelerated, and the implants can be finally completely converted into autologous bone tissues.

The invention relates to a method for constructing tissue-engineering skin. The construction method adopts an autocrine extracellular matrix of fibroblast as a biological scaffold of the tissue-engineering skin. The method utilizes the extracellular matrix excreted by the cultured fibroblast as the biological scaffold and human epidermal cells as seed cells, and the cultured tissue-engineering skin is closer to the structure of natural skin. The method has the advantages that: firstly, the method is simple and convenient, and favorable for synthesis and mass production of products because thematerials take the form of liquid; secondly, the obtained product has the structure which is closer to the natural skin, and the method is favorable for improving the sensibility and the precision ofprediction of the toxicity of compounds; thirdly, the product does not contain additional biomaterials, so that the risk of cross infection of zoonosis cannot occur; and fourthly, the obtained product is convenient for quality control, so that the variation between batches is reduced.

The invention provides blank polylactic acid-glycolic acid copolymer microspheres which are prepared by a water-oil-water emulsion process. The PLGA microspheres are prepared from PLGA powder by using excellent dissolving capacity of dichloromethane and emulsification of polyvinyl alcohol. Through representation observation and particle size determination of the microspheres, the diameter and the surface sign of a biological scaffold material are obtained; and the diameters of the PLGA blank microspheres accord with the injection size. Through CCK-8 determination and lactic dehydrogenase determination, the microspheres have no toxicity on attached mesenchymal stem cells, and do not affect the growth activity. Compared with chitosan particles, the microspheres are simple in preparation method, high in repetitive rate, clear structure, and easy to degrade in a living body, do not affect microenvironment metabolism, and can used as good cell carriers for different treatment targets in tissue regeneration engineering.

The invention discloses a self-adaptive direct slicing method for a preparing biological scaffold. The method is secondary development using VC++ and conducted on a SolidWorks2011 platform, and the core concept provided by the method and a special treatment mechanism adopted by aiming at the three-dimensional mold characteristics of the biological scaffold can be particularly achieved by relying on other software and hardware platforms. The self-adaptive direct slicing method for the preparing biological scaffold is mainly divided into four parts including pre-treatment, thickness self-adaptive treatment, direct slicing and post-treatment. Two constituent parts, namely an ESD substep and an IAD substep, of the thickness self-adaptive treatment are two completely independent steps and are not overlapped on operand and operational objects. Algorithm or operation which wastes system resources and spends a lot of time during the preparation process is effectively avoided, efficiency can be improved, and precision can be guaranteed.

The invention belongs to the biotechnical field and in particular to tissue engineering skin containing appendage and a preparation method thereof. The preparation method comprises the following steps: performing transfection of a urine cell to induce to generate a multifunctional stem cell; continuously inducing the multifunctional stem cell to generate a mesenchymal stem cell; differentiating the mesenchymal stem cell to form an epidermis cell, a fibroblast and a hair follicle cell; and constructing tissue engineering double-layered skin containing the appendage with a biological stent material amniotic membrane. The seed cell of the tissue engineering skin prepared by the preparation method is the urine cell which is convenient to obtain and short in cultivation period. The hair follicle structure which is contains facilitates union of a wound, and the tissue engineering skin containing the appendage provided by the invention lays a foundation for follow-up development of the tissue engineering skin containing the appendage.

The present invention provides for novel peptoid oligomers that are capable of self-assembling into two-dimensional sheet structures. The peptoid oligomers can have alternately hydrophilic or polar side-chains and hydrophobic or apolar side-chains. The peptoid oligomers, and the two-dimensional sheet structures, can be applied to biological applications where the peptoid plays a role as a biological scaffold or building block. Also, the two-dimensional sheet structures of the present invention can be used as two-dimensional nanostructures in device applications.

The invention discloses a dual-network hydrogel composition, a dual-network hydrogel biological scaffold, a preparation method of the scaffold, and an application of the composition. The double-network hydrogel composition comprises aldehyde hyaluronic acid, carboxymethyl chitosan, vinyl gelatin and a photo-crosslinking initiator, wherein a mass ratio of the aldehyde hyaluronic acid to the carboxymethyl chitosan is 1:1 to 5:1, and a mass ratio of the vinyl gelatin to the carboxymethyl chitosan is 1:2 to 5:1. The aldehyde hyaluronic acid and carboxymethyl chitosan in the composition are reactedto obtain a first network, the vinyl gelatin photo-initiates free radical polymerization to prepare a second network structure, the formed double-network hydrogel biological scaffold has good structural stability, good cell compatibility, good biological safety and excellent structural fidelity, and the compression modulus of the hydrogel biological scaffold can be adjusted to 10-100 KPa by controlling the proportions of all the components, so the hydrogel biological scaffold is suitable for printing various tissue bodies, and the activity of printed cells is 80% or above, thereby the application of the cell biological scaffold material is further expanded.

The invention discloses a preparation method and an application of a biological scaffold material and SVF assistant adipose tissue. Under an aseptic condition, the biological scaffold material, a stromal vascular fraction (SVF) prepared by digesting purified adipose granules with a medical-graded collagenase, and the purified adipose particles are evenly mixed according to a certain proportion to prepare an adipose graft for filling. The SVF is a mixture of adipose mesenchymal stem cells and various cells, is transplanted after being mixed with autogenous adipose particles, and can be continued to be developed into a mature adipose tissue in vivo; the biological scaffold material can provide a support for transplanted adipose, allows the transplanted tissue to be prone to revascularization, and improves the survival rate of the transplanted tissue; the transplanted tissue is suitable for being injected with a 16 G or 18 G needle, facilitates clinical application, and can be used for repairing of concave deformity (such as tempora and cheek concave), beautifying packing of faces and chests, soft tissue function reconstruction and the like; and especially in the beautifying packing, the prepared transplanted adipose tissue can promote shape recovery and prevent liquefactive necrosis.

The invention belongs to the technical field of tissue engineering and discloses tissue engineering full-layer skin and a preparing method thereof. The tissue engineering full-layer skin comprises epidermal cells, dermal cells and a biological scaffold material. The skin is obtained by compounding the dermal cells in the biological scaffold material, inoculating the surface with the epidermal cells and carrying out culturing and preparing. The tissue engineering full-layer skin is provided with a dermis layer and an epidermal layer which are provided with activity cells, the epidermal layer contains stratum corneum and is approximate to the normal skin, after transplanting is carried out, complete human skin with a function can be regenerated, the regenerated skin has skin appendant organs such as hair and the sebaceous gland, and the skin can be used for treating skin defects caused by inflammation, ulcer, burns, scalds and the like, and can also be used for preparing a skin model for disease study, medicine testing and screening, cosmetic testing and the like.

Provided herein are scaffolds and methods useful to promote the formation of functional clusters on a tissue, for example, motor endplates (MEPs) or a component thereof on skeletal muscle cells or tissue, as well as the use of scaffolds so produced for repairing a tissue injury or defect.

The invention discloses a culture method for the composition of a periosteal biological scaffold and allogenic seed cells. The culture method comprises the following steps: the non-immunogenic periosteal biological scaffold and the seed periosteal cells are prepared; the non-immunogenic periosteal biological scaffold is sealed after low-temperature drying; after gamma-ray disinfection of the non-immunogenic periosteal biological scaffold, the seed periosteal cells, with the concentration of 1*106 per milliliter, are dripped on the surface of the disinfected scaffold; a culture medium is added after the periosteal cells gradually permeate into the non-immunogenic periosteal biological scaffold together with a suspension, the medium change is carried out every 2 to 3 days, and the in-vitro composite culture lasts for one week. The periosteal biological scaffold obtained through the method has the characteristics of complete removal of immunogenic cells, as well as good preservation of the structure and major components of extracellular matrix; effective composition of the allogenic periosteal cells and the biological scaffold can be achieved, so that a biological composite scaffold material can be provided for tissue engineering researches on bone defects and bone nonunion.

The invention discloses a use of a kit in the preparation of a medicine. The medicine is for treating full thickness skin injuries. The kit includes: a first reagent including an exosome and a cytokine; and a second reagent including a biological scaffold and a stem cell. The kit can effectively promote the repairing of skin injuries and the healing of skin wounds.