84 results about "Biologic scaffold" patented technology

The invention discloses a biological functional scaffold system driven by dual-mode printing, relates to the technical field of biomedicine, and is used for providing more accurate control on the aspects of porosity, cell attachment and surface modification. By integrating two advanced manufacturing technologies of 3D printing and aerosol spraying, the porosity and surface characteristics of the stent can be accurately controlled in the same printing process, and the cell attachment and proliferation environment is optimized. The cooperative working mechanism greatly improves the biocompatibility and mechanical strength of the biological scaffold, and further effectively promotes the tissue regeneration and repair efficiency. The whole manufacturing process is more efficient and accurate due to introduction of the control unit. The control unit automatically adjusts the printing speed and the spraying pressure based on real-time monitoring feedback, the uniformity and stability of the support are ensured, the error rate in the production process is reduced, and therefore the consistency and reliability of products are improved.

The invention discloses an intelligent integrated system for manufacturing a biological tissue engineering scaffold, relates to the technical field of biomedicine, and is used for improving the biocompatibility, mechanical strength and cell adhesion capacity of a biological scaffold. The plasma generator comprises a microwave generator, a precise electrode and an efficient gas flowing unit; the integrated manufacturing module comprises a 3D printing unit, a plasma enhanced chemical vapor deposition (PECVD) unit and a cooperative control unit; the monitoring and control system comprises an intelligent temperature and humidity sensor, a gas component monitor and an automatic control unit; the data management and optimization module comprises a biological safety evaluation unit, a personalized design and simulation unit, a material selection and management unit, a degradable material processing unit and a dynamic feedback and adjustment unit. According to the invention, low-temperature plasma treatment, 3D printing and PECVD technologies are fused, and a highly intelligent and integrated manufacturing platform is constructed.

The invention discloses a magnesium-containing nanoparticle modified biological scaffold composite material as well as a preparation method and application thereof. The magnesium-containing nano particle modified biological scaffold composite material comprises a biological scaffold material and magnesium-containing nano particles modified on the biological scaffold material, the magnesium-containing nano particles comprise nano magnesium oxide particles and / or nano magnesium oxide-polyphenol compound particles, the nano magnesium oxide-polyphenol compound particles are of a shell-core structure, and the shell-core structure is of a hollow structure. Comprising magnesium oxide serving as a core and a polyphenol compound shell layer coating the surface of the magnesium oxide. The magnesium-containing nanoparticle-modified biological scaffold composite material provided by the invention can prolong the degradation and release of magnesium ions, promote improvement of microenvironment problems such as inflammation and active oxygen outbreak after spinal cord injury, regulate and control oxidative stress and inflammatory response, promote nerve regeneration and motor function recovery, and improve the survival rate of spinal cord injury. The problem that nanoparticles stop in a spinal cord injury area is effectively solved, and support is provided for migration, growth and differentiation of endogenous neural stem cells.

The invention discloses a biological scaffold viscoelasticity evaluation method and related equipment, and aims to overcome the defects of an existing biological scaffold viscoelasticity test method and biological tissue suitability quantitative evaluation. The method comprises the following steps: determining a target implantation part, and acquiring force relaxation experimental data of a biological scaffold by using an atomic force microscope; judging a contact point of the probe and the bracket based on the data; determining the moment when the force signal is stable after the support is compressed to the preset deformation according to the contact point as a force relaxation starting point; calculating the characteristic time when the force value is attenuated to half by taking the starting point as a timing starting point as a microscopic viscoelasticity index; and finally, realizing quantitative evaluation of viscoelasticity suitability by comparing the characteristic time of the stent and the target tissue.

The invention provides a nano transdermal sustained-release biological scaffold as well as a preparation method and application thereof, and belongs to the technical field of biomedical materials. The nano transdermal sustained-release biological scaffold provided by the invention comprises the following components: a load and a nano carrier, the load comprises mussel mucin, trehalose and Ectoine; the mass ratio of the mussel mucin to the trehalose to the Ectoine is (4 to 6) to (0.5 to 1.5) to (0.5 to 2.0). Through the innovative design of a quaternary composite system of mussel mucin, trehalose, Ectoine and a nano-carrier, the three technical problems of short slow release period, insufficient targeting property and difficulty in large-scale production in a wound repair material in the prior art are successfully solved.

The growth factor profile, connective tissue matrix constituents, and immunoprivileged status of urodele extracellular matrix (ECM) and accompanying cutaneous tissue, plus the presence of antimicrobial peptides there, render urodele-derived tissue an ideal source for biological scaffolds for xenotransplantation. In particular, a biological scaffold biomaterial can be obtained by a process that entails (A) obtaining a tissue sample from a urodele, where the tissue comprises ECM, inclusive of the basement membrane, and (B) subjecting the tissue sample to a decellularization process that maintains the structural and functional integrity of the extracellular matrix, by virtue of retaining its fibrous and on-fibrous proteins, glycoaminoglycans (GAGs) and proteoglycans, while removing sufficient cellular components of the sample to reduce or eliminate antigenicity and immunogenicity for xenograft purposes. The resultant urodele-derived biomaterial can be used to enhance restoration of skin homeostasis, to reduce the severity, durations and associated damage caused by post-surgical inflammation, and to promote progression of natural healing and regeneration processes. In addition, the biomaterial promotes the formation of remodeled tissue that is comparable in quality, function, and compliance to undamaged human tissue.

Disclosed is use of a Histatin-1 polypeptide in promotion of cartilage regeneration or repair. By preparing a combined reagent containing a Histatin-1 polypeptide, a biological scaffold material and the like, mainly a linear Hst1 is loaded on gelatin methacrylate so as to be used for repairing cartilage damage or promoting cartilage growth; meanwhile, disclosed is use of a Histatin-1 polypeptide in promotion of the expression of one or more of bone differentiation indexes, including a collagen fiber, glycosaminoglycan, type II collagen and aggrecan.

The invention relates to the technical field of ceramic aerogel, in particular to hydroxyapatite biological ceramic aerogel with a directional hole structure as well as a preparation method and application of the hydroxyapatite biological ceramic aerogel. The preparation method comprises the following steps: firstly, adding hydroxyapatite particles, a sintering aid and a binder into a water solvent according to a certain mass ratio to prepare aerogel ink; and secondly, filling the ink into an extrusion needle tube of a direct-writing 3D printing technology, and carrying out customized printing on hydroxyapatite aerogel on a liquid nitrogen cooled copper plate by adopting an ice template method as an assistance. After freeze drying and sintering, the biological ceramic aerogel with the directional hole structure on the micro scale is finally obtained. The oriented pore biological ceramic aerogel has excellent mechanical properties and biocompatibility, and has good prospects in the fields of biological scaffolds, bone tissue engineering, biosensor substrates and the like. The method for preparing the hydroxyapatite biological ceramic aerogel by using the ice template method to assist the 3D printing technology is low in cost, simple to operate and wide in application range, and has great application potential.

The invention relates to the technical field of biological scaffolds, in particular to a silk fibroin-based biological 3D intelligent response scaffold and a preparation method and application thereof.The silk fibroin-based biological 3D intelligent response scaffold adopts a three-layer bionic gradient structure of an oriented fiber membrane, a porous sponge layer and an interface layer, simulates natural tissues, and has the advantages that the bionic gradient structure is improved; mechanical guidance, cell inhabitation and biological activity interfaces are provided respectively, and high-quality regeneration of blood vessels, nerves and skin is promoted synergistically. According to the present invention, the pH / enzyme / temperature triple response unit is provided, such that the lesion microenvironment can be perceived, the targeted release can be triggered, the problem of high burst release rate of the traditional physical drug loading can be solved, and the on-demand accurate drug delivery can be achieved. The SF / HPMC gel is adopted for 3D printing, the printing precision is high, and the porosity difference is 1t; the product performance is stable, and the pore structure can be precisely designed and customized according to the regeneration requirements of different tissues (such as blood vessels, nerves and skin).

The invention discloses a preparation method of composite microspheres for regulating and controlling a local inflammation microenvironment and promoting nerve regeneration. The method comprises the following steps: preparing liposome loaded with ginsenoside Rg1; preparing a GM microsphere loaded with a stromal cell-derived factor 1 alpha (SDF1 alpha); and preparing the inflammation regulation nerve regeneration promoting composite microspheres. The composite microsphere can reduce inflammatory response, reduce scar tissue formation, promote angiogenesis, continuously release SDF1 alpha and promote endogenous stem cell recruitment and neural differentiation. Therefore, the composite microsphere is a functional biological scaffold for firstly regulating and controlling local microenvironment inflammation of spinal cord injury and then promoting collection and differentiation of endogenous stem cells for innovative sequential inflammation regulation and nerve regeneration, and a new strategy is provided for treating spinal cord injury by tissue engineering.

The invention relates to the technical field of food processing, in particular to a high-yield nutrient 3D printing biological scaffold prepared on the basis of neurospora crassa spores and bean dregs. The biological scaffold prepared by the invention can form a closed-loop system of agricultural wastes, biological carriers and high-value products. The system has the characteristics of high efficiency, sustainability, flexibility, controllability and the like and the capabilities of modular design and dynamic regulation and control, programmable regulation and control of active compound synthesis can be realized through material-cell interaction, and a novel manufacturing mode is provided for synthesis and precise biosynthesis of nutrient substances such as fungal protein and carotenoid.

The present disclosure relates to compositions, apparatus and methods for generating one or more scaffolds, including: mixing a hydrogel material and / or an extracellular matrix (ECM) protein in an aqueous solvent to generate an aqueous process solution; and cryoelectrospinning the aqueous process solution onto a plurality of conductive probes extending from a conductive surface of a collector plate disposed within a process chamber under conditions sufficient to generate one or more scaffolds configured to mimic a preselected soft tissue decellularized extracellular matrix. Scaffold compositions are also provided having preselected or tuned characteristics.

The invention provides a porous biological scaffold and a preparation method thereof, and the porous biological scaffold is prepared by pre-freezing and freeze-drying a mixed solution formed by a hydrophobic polyester material and a hydrophilic polymer material, the stent has a three-dimensional porous structure, can be compressed to be smaller than the original volume in a dry state, and can recover to the original volume after being released from a compressed state. According to the invention, the hydrophobic polyester material and the hydrophilic polymer material cooperate with each other, so that the material has unique dynamic response performance, specifically, the material has good compressibility in a dry state, can be compressed to be smaller than the original volume so as to be conveyed into a body, and can be recovered to the original volume before compression after being implanted into the body; and plugging, filling or mechanical supporting functions are realized.

The invention relates to the technical field of biology, and particularly discloses a recombinant humanized II-type collagen biological scaffold and application thereof in cartilage repair. The recombinant humanized II-type collagen biological scaffold is formed by freezing and drying a polypeptide solution, and the amino acid sequence of polypeptide comprises n repetitive units, the repetitive unit comprises any one of the following amino acid sequences (1)-(3) or a variant sequence thereof: (1) an amino acid sequence as shown in SEQ ID NO.1; (2) an amino acid sequence having at least 50%-99% sequence identity with the amino acid sequence as shown in SEQ ID NO.1; (3) a variant sequence obtained by performing mutation of one or more amino acid residues on the amino acid sequence as shown in SEQ ID NO.1; wherein n is an integer greater than or equal to 1. The biological scaffold disclosed by the invention does not contain a cross-linking agent and an organic solvent, has high biocompatibility and can quickly repair cartilage tissues.

Encasement structures and methods of customizing patient drug delivery profiles using an encasement structure are described herein. Encasement structures can be configured to receive an implantable medical device and physicians can implant the medical devices within the encasement structures. Encasement structures can include at least one sheet of a bioscaffold material and one or more depots. Depots can be configured to release an active agent, such as an antibiotic, to the medical device within the encasement structure and / or the surrounding tissue. The depots can be insertable into or integrated with the at least one sheet of bioscaffold material.

The application belongs to the technical field of 3D printing biomaterials, and particularly relates to a collagen biomaterial ink for in-situ real-time 3D printing by one-step method as well as a preparation method and application thereof. The collagen biomaterial ink is prepared by mixing a collagen solution, methacrylic anhydride and a photoinitiator, and is cured under irradiation of blue light with a wavelength of 405 nm; the collagen biomaterial ink has good extrudability, and can be 3D printed to obtain a fine micron-level structure; the biological scaffold printed by the collagen biomaterial ink has good biocompatibility and bioactivity, and can significantly promote the repair of skin damage.

The invention discloses a preparation method of a vascular organ chip based on a degradable bionic stent. The degradable biological scaffold (PCL-FBE scaffold for short) is obtained by mixing a swimming bladder extract and polycaprolactone and carrying out electrostatic spinning. On the basis, a vascular organ chip which can be integrated in a micro-fluidic system is further constructed, and a whole process for completing three-dimensional dynamic culture of vascular organs by using the chip is established. According to the present invention, the swim bladder extract and polycaprolactone are compounded to form the electrospinning scaffold, such that the defects of the mechanical property, the degradability and the biocompatibility of the traditional bionic scaffold are successfully overcome, the organoid culture effect is significantly improved, and the efficient and sustainable innovation platform is provided for the tissue engineering and the regenerative medicine.

A method of modifying a surface of a three-dimensional (3D) article includes immersing at least a portion of the 3D article in a buffer solution of a functionalized peptide such that the functionalized peptide reacts with a reactive group on the surface of the 3D article; and washing at least a portion of the 3D article to remove unreacted functionalized peptides. The surface-modified 3D article comprises a plurality of peptides covalently bonded to a surface of the 3D article by cysteine bridging. The surface-modified 3D articles can be used as scaffolds for constructing biological tissues or implants.

The present application relates to a bioprinting system and method for precise cell positioning, and relates to the field of three-dimensional printing technology. The system includes: a three-dimensional motion module, which is used to achieve precise movement of the print head; a printing module, which includes a print head, which includes a printing nozzle, and the printing module is used to construct a biological scaffold and / or eject cells; an information acquisition module, which includes a camera for real-time positioning of the physical position and / or cell position of the biological scaffold; a software module, which is used to achieve motion control of the print head. The bioprinting system and method for precise cell positioning of the present application achieves precise cell positioning by precisely printing a single cell or a single row of cell sequences onto a target biological scaffold, providing the necessary foundation for the subsequent orderly growth, proliferation and signal transduction of cells.

The invention provides a visual biological scaffold capable of discharging percolate in a self-pumping manner and a preparation method of the visual biological scaffold. The visual biological scaffold comprises the following components in parts by weight: 5-20 parts of a hydrophobic high polymer material, 80-95 parts of a volatile solvent, 5-10 parts of a hydrophobic component, 3-15 parts of a hydrophilic high polymer material and 0.1-15 parts of an antibacterial agent. A hydrophilic and hydrophobic material is co-spun through electrostatic spinning, the material is endowed with certain hydrophilicity, the attachment and proliferation rate of cells can be increased, wound healing is promoted, a 3D network microchannel is formed, and the channel has a self-pumping drainage effect and can be used as a miniature infusion pump; the antibacterial substance is carried through electrostatic spinning, the antibacterial substance is physically wrapped in the fiber or dispersed in a fiber matrix or adsorbed on the surface of the fiber, and the antibacterial substance has a slow release effect, so that efficient and continuous sterilization is achieved, and wound infection is prevented; the device has the characteristic of visualization, and the wound healing degree can be observed; the electrostatic spinning in-situ forming technology is adopted, and the biological scaffold deposited on the wound has adhesiveness and does not need to be fixed additionally.

The invention belongs to the technical field of biomedical materials, and discloses a bone tissue repair material for promoting vascularization and new bone generation and a preparation method thereof. The bone tissue repair material is composed of a decalcified bone matrix and a degradable biological source gel material. The repair material has a loose porous three-dimensional structure similar to that of natural cancellous bone, and the temperature-sensitive gel material filled in the pore structure of the repair material can form gel in situ in vivo and is attached to pores of a decalcified bone matrix, so that cell migration, adhesion and proliferation can be promoted, the degradation rate of gel in vivo can be reduced, and the repair material has a good application prospect. The continuous release of the pro-angiogenesis factor is ensured. Good biocompatibility is achieved, and cytotoxicity and immunogenicity are avoided; meanwhile, in the slow degradation process, angiogenesis promoting factors are continuously released for a long time, and angiogenesis and new bone generation are promoted. Therefore, the implanted biological scaffold prepared from the bone tissue repair material has excellent bone repair and regeneration promotion capability and wide application prospect.

The invention provides a small-caliber blood vessel biological scaffold capable of adjusting stress relaxation and a preparation method of the small-caliber blood vessel biological scaffold. The biological scaffold is of a tubular structure which is formed by arranging micron fibers and nanofibers in a crossed mode, is arranged in a net shape and is provided with a layered tube wall. The small-caliber blood vessel biological scaffold has adjustable stress relaxation performance, biodegradability, bionic viscoelasticity and excellent surgical suture performance, endogenous cells can be guided to be clustered and self-assembled, a small-caliber blood vessel graft with the deformation behavior close to that of a natural blood vessel is formed, and the small-caliber blood vessel biological scaffold has the advantages of being simple in structure and convenient to use. And the double bottleneck that the mechanical property and the tissue regeneration function of the traditional stent are difficult to consider is solved.

This invention relates to a decellularized oral collagen plug and its preparation method. The plug comprises an upper decellularized matrix membrane and a lower decellularized sponge plug. The preparation method is as follows: pretreated fresh animal-derived membrane material is treated with trypsin solution and sodium hydroxide solution, washed with purified water to obtain a decellularized matrix membrane; a portion of the obtained decellularized matrix membrane is placed in a low-temperature tissue ball mill, pulverized, homogenized, filtered, and washed with purified water to obtain a decellularized collagen solution; the decellularized collagen solution is poured into a freeze-drying mold, defoamed under low-temperature vacuum, covered with the decellularized matrix membrane, and after vacuum defoaming, freeze-dried and vacuum thermally cross-linked to obtain the finished collagen plug. This invention's decellularized oral collagen plug is suitable for filling and covering wounds after tooth extraction. The upper decellularized matrix membrane effectively prevents fibroblasts and epithelial cells from invading bone defect areas, while the lower decellularized sponge plug stabilizes blood clots, provides a biological scaffold, reduces postoperative complications, and promotes wound healing.

The growth factor profile, connective tissue matrix constituents, and immunoprivileged status of urodele extracellular matrix (ECM) and accompanying cutaneous tissue, plus the presence of antimicrobial peptides there, render urodele-derived tissue an ideal source for biological scaffolds for xenotransplantation. In particular, a biological scaffold biomaterial can be obtained by a process that entails (A) obtaining a tissue sample from a urodele, where the tissue comprises ECM, inclusive of the basement membrane, and (B) subjecting the tissue sample to a decellularization process that maintains the structural and functional integrity of the extracellular matrix, by virtue of retaining its fibrous and on-fibrous proteins, glycoaminoglycans (GAGs) and proteoglycans, while removing sufficient cellular components of the sample to reduce or eliminate antigenicity and immunogenicity for xenograft purposes. The resultant urodele-derived biomaterial can be used to enhance restoration of skin homeostasis, to reduce the severity, durations and associated damage caused by post-surgical inflammation, and to promote progression of natural healing and regeneration processes. In addition, the biomaterial promotes the formation of remodeled tissue that is comparable in quality, function, and compliance to undamaged human tissue.

The invention discloses a selenium-iron co-doped hydroxyapatite reinforced polylactic acid scaffold and a preparation method thereof, and the preparation method comprises the following steps: co-doping SeO3 < 2-> and Fe < 3 + > into HAP through a co-precipitation method to obtain SF-HAP powder; sF-HAP and PLLA are dispersed in ethyl alcohol, and PLLA / SF-HAP composite powder is obtained through stirring, centrifuging, drying and the like; the PLLA / SF-HAP biological scaffold is prepared through an additive manufacturing technology, in order to endow the PLLA / HAP bone scaffold with an anti-tumor function, Fe is doped into HAP, a Fenton reaction can be promoted in an osteosarcoma environment so that ROS can be generated to kill tumor cells, but overexpressed GSH existing in the tumor cells can consume the ROS to weaken the anti-tumor effect, and doping of Se and Fe can reduce the crystallinity of HAP crystals, so that the anti-tumor effect is improved. The SeO3 < 2-> and the Fe < 3 + > are added into the tumor cells, so that the sustained release of functional ions is facilitated, meanwhile, the consumption of the SeO3 < 2-> and the Fe < 3 + > on the over-expressed GSH and H2O2 in the tumor cells can effectively destroy the redox balance in the tumor cells, and the death of the tumor cells is promoted.

The present invention provides a biological scaffold loaded with acellular membrane sheets, a preparation method and an application thereof. The biological scaffold loaded with acellular membrane sheets comprises: a biological scaffold, a decellular membrane sheet for promoting alveolar bone regeneration and a decellular membrane sheet for promoting periodontal ligament regeneration respectively carried on both sides of the biological scaffold. The biological scaffold loaded with acellular membrane sheets is used to prepare a periodontal regeneration module. The biological scaffold loaded with acellular membrane sheets comprises: a biological scaffold, a decellular membrane sheet for promoting alveolar bone regeneration and a decellular membrane sheet for promoting periodontal ligament regeneration respectively carried on both sides of the biological scaffold. The decellular membrane sheet for promoting alveolar bone regeneration and the decellular membrane sheet for promoting periodontal ligament regeneration are used to provide different regeneration environments on both sides of the biological scaffold, thereby providing two independent regeneration environments for periodontal ligament regeneration and alveolar bone regeneration, respectively, thereby achieving periodontal regeneration.

The invention relates to the technical field of biomedical engineering, in particular to a method for predicting stress distribution of a biological scaffold by using a recursive network, which comprises the following steps: S1, acquiring geometric structure parameters and in-vitro culture environment parameters of the biological scaffold; s2, constructing a three-dimensional geometric model of the biological scaffold, and calculating the porosity of the scaffold and the shape characteristics of the support columns; s3, simulating a flow velocity field, a pressure field and shear stress distribution in the support; s4, calculating initial stress distribution of the biological scaffold under the action of fluid mechanics; s5, calculating the additional stress generated in the cell growth process, and iteratively updating the stress distribution of the stent through the recurrent neural network. According to the method, by combining the recurrent neural network and the cell proliferation kinetic model, the stress distribution of the biological scaffold in the cell growth process is accurately predicted, the scaffold design is optimized, and the biocompatibility and the mechanical stability of the scaffold are improved.

The invention relates to the field of bone repair, and discloses a 3D printing bone-imitating biological scaffold and a preparation method thereof.The preparation method comprises the steps that firstly, ammonium bromide is added into a mixture of deionized water and absolute ethyl alcohol, and an ammonium bromide solution is obtained; sequentially adding an ammonia solution, tetraethoxysilane, triethyl phosphate, calcium nitrate and sodium vanadate, continuously stirring, centrifuging, and collecting a precipitate; washing, aging, heating, cooling, grinding, crushing, and screening to obtain V-MBG powder; preparing a TA solution and a V-MBG solution, mixing and putting the TA solution and the V-MBG solution, and freeze-drying to obtain TA-coated V-MBG powder; preparing a trichloromethane solution of PLGA (poly (lactic-co-glycolic acid)), adding the V-MBG powder, fully stirring, and pumping out the trichloromethane solution to obtain solid slurry; printing an inner-layer stent and a hollow-tube outer-layer stent to obtain a bone-imitating biological stent, and soaking the bone-imitating biological stent in a TA solution to obtain a PLGA / TA-V-MBG composite stent; the problems that in the prior art, the bone repairing effect is poor, the bone-like cell proliferation and differentiation effect is poor, and bone defect repairing caused by bone tumor treatment cannot be achieved are solved.