26 results about "Biologic scaffold" patented technology

The invention relates to the technical field of biological scaffolds, in particular to a silk fibroin-based biological 3D intelligent response scaffold and a preparation method and application thereof.The silk fibroin-based biological 3D intelligent response scaffold adopts a three-layer bionic gradient structure of an oriented fiber membrane, a porous sponge layer and an interface layer, simulates natural tissues, and has the advantages that the bionic gradient structure is improved; mechanical guidance, cell inhabitation and biological activity interfaces are provided respectively, and high-quality regeneration of blood vessels, nerves and skin is promoted synergistically. According to the present invention, the pH / enzyme / temperature triple response unit is provided, such that the lesion microenvironment can be perceived, the targeted release can be triggered, the problem of high burst release rate of the traditional physical drug loading can be solved, and the on-demand accurate drug delivery can be achieved. The SF / HPMC gel is adopted for 3D printing, the printing precision is high, and the porosity difference is 1t; the product performance is stable, and the pore structure can be precisely designed and customized according to the regeneration requirements of different tissues (such as blood vessels, nerves and skin).

The invention relates to the technical field of food processing, in particular to a high-yield nutrient 3D printing biological scaffold prepared on the basis of neurospora crassa spores and bean dregs. The biological scaffold prepared by the invention can form a closed-loop system of agricultural wastes, biological carriers and high-value products. The system has the characteristics of high efficiency, sustainability, flexibility, controllability and the like and the capabilities of modular design and dynamic regulation and control, programmable regulation and control of active compound synthesis can be realized through material-cell interaction, and a novel manufacturing mode is provided for synthesis and precise biosynthesis of nutrient substances such as fungal protein and carotenoid.

The invention provides a porous biological scaffold and a preparation method thereof, and the porous biological scaffold is prepared by pre-freezing and freeze-drying a mixed solution formed by a hydrophobic polyester material and a hydrophilic polymer material, the stent has a three-dimensional porous structure, can be compressed to be smaller than the original volume in a dry state, and can recover to the original volume after being released from a compressed state. According to the invention, the hydrophobic polyester material and the hydrophilic polymer material cooperate with each other, so that the material has unique dynamic response performance, specifically, the material has good compressibility in a dry state, can be compressed to be smaller than the original volume so as to be conveyed into a body, and can be recovered to the original volume before compression after being implanted into the body; and plugging, filling or mechanical supporting functions are realized.

The invention relates to the technical field of biology, and particularly discloses a recombinant humanized II-type collagen biological scaffold and application thereof in cartilage repair. The recombinant humanized II-type collagen biological scaffold is formed by freezing and drying a polypeptide solution, and the amino acid sequence of polypeptide comprises n repetitive units, the repetitive unit comprises any one of the following amino acid sequences (1)-(3) or a variant sequence thereof: (1) an amino acid sequence as shown in SEQ ID NO.1; (2) an amino acid sequence having at least 50%-99% sequence identity with the amino acid sequence as shown in SEQ ID NO.1; (3) a variant sequence obtained by performing mutation of one or more amino acid residues on the amino acid sequence as shown in SEQ ID NO.1; wherein n is an integer greater than or equal to 1. The biological scaffold disclosed by the invention does not contain a cross-linking agent and an organic solvent, has high biocompatibility and can quickly repair cartilage tissues.

Encasement structures and methods of customizing patient drug delivery profiles using an encasement structure are described herein. Encasement structures can be configured to receive an implantable medical device and physicians can implant the medical devices within the encasement structures. Encasement structures can include at least one sheet of a bioscaffold material and one or more depots. Depots can be configured to release an active agent, such as an antibiotic, to the medical device within the encasement structure and / or the surrounding tissue. The depots can be insertable into or integrated with the at least one sheet of bioscaffold material.

The application discloses application of a Histatin-1 polypeptide in preparation of a preparation for promoting sciatic nerve regeneration or repair, and an amino acid sequence of the Histatin-1 polypeptide is shown as SEQ ID NO. 1. The Histatin-1 polypeptide can promote expression of a nerve differentiation index, promote recovery of a functional index after sciatic nerve injury, and promote recovery of a histological index after sciatic nerve injury, for example, recovery of myelin structure and morphology. In addition, in combination with gelatin methacrylate (Gel-MA) as a biological scaffold, slow release of the Histatin-1 polypeptide can be realized, continuous repair support can be provided for a nerve injury site, myelin repair effect is further optimized, a more efficient and more durable solution is provided for treatment of sciatic nerve injury, and the application has a wide application prospect in the field of treatment of myelinated nerve injury.

The present application belongs to the technical field of biological scaffolds, and particularly relates to a preparation method of a porous biomimetic bone scaffold and the biomimetic bone scaffold. The preparation method of the porous biomimetic bone scaffold comprises the following steps: mixing a hydrogel main material, water, a crosslinking agent, an alkyl amine compound and an optional complex high polymer material; and performing directional freezing treatment on the obtained mixed product, wherein the hydrogel main material is selected from one or more than two of silk fibroin, collagen and gelatin, and the complex high polymer material is selected from one or more than two of hyaluronic acid, chitosan and other biological source natural polymer and synthetic organic high polymer compound. The porous biomimetic bone scaffold with certain pore size directional micropore channels and nanochannels formed along the side walls of the directional micropore channels can be prepared by the method.

The application relates to a preparation method of a bacterial cellulose-based multilayer structure functional bionic scaffold, which comprises the following steps: firstly, injecting a biomaterial solution into a silica gel model with a microchannel system to perform freeze drying to obtain a porous biological scaffold; secondly, performing crosslinking treatment on the porous biological scaffold; thirdly, inoculating a culture bacteria solution with a Xylobacter concentration of 10 9 ~ 10 15 individuals / mL on the crosslinking-treated porous biological scaffold, and performing static culture and freeze drying to obtain a bacterial cellulose-based composite scaffold; and finally, adopting a coating solution to coat the bacterial cellulose-based composite scaffold through a microchannel system, and performing demolding treatment to obtain the bacterial cellulose-based multilayer structure functional bionic scaffold. The application provides a functional coating process for the multilayer bionic scaffold by using the shrinkage space of the treated material, the micro-pore channel and the injection method of the patterned drainage network, effectively prevents the penetration of the coating solution, and realizes the perfect solidification of the coating solution on the scaffold.

The invention discloses a biological scaffold energy storage and dissipation modulus analysis method and related equipment, and aims to overcome the defect that in the prior art, key viscoelastic mechanical characteristics of a biological scaffold and target spinal cord tissue of the biological scaffold under micro-scale and dynamic conditions cannot be accurately and comprehensively represented and analyzed. The method comprises the following steps: acquiring force-time curve data at a micro-scale, and aligning a time zero point to a relaxation starting point to obtain regular sequence data; fourier transform is carried out on the regular data to obtain complex shear moduli, wherein the complex shear moduli respectively comprise an energy storage modulus and a dissipation modulus; and generating a frequency spectrum curve based on the complex shear modulus and obtaining a modulus curve. The comprehensive characterization of the wide-frequency-domain dynamic viscoelasticity of the biological scaffold under the microscopic scale is realized, and accurate data support is provided for mechanical property optimization of the scaffold.

The application discloses a conductive hydrogel material and a preparation method and application thereof. The preparation method comprises the following steps: subjecting a first high polymer compound and a second high polymer compound to a Michael addition reaction and a cross-linking reaction to prepare a hydrogel precursor; and subjecting the hydrogel precursor to compression mixing with a conductive carbon-based material in a mold, standing, demolding, and preparing the conductive hydrogel material. The preparation method mixes the conductive carbon-based material with the hydrogel precursor in the mold, improves the conductivity of the carbon-based conductive hydrogel prepared by a traditional method, and makes the carbon-based conductive hydrogel have a wider application space in the field of biosensing. Meanwhile, the conductive hydrogel material prepared by the application has good mechanical properties and certain adhesion, the hydrogel components are safe and have good biocompatibility, the conductive hydrogel material can better simulate a cell growth environment as a biological scaffold material, and the cell growth can be regulated through external stimulation; and the material has good mechanical properties and good pressure sensitivity, and is suitable for being used as a flexible biosensing electrode material.

The present application relates to a double-nanoparticle co-stable 3D printing Pickering emulsion ink, a preparation method and an application, wherein trace functional element hydroxyapatite (X-HA) and gelatin nanoparticles (GNPs) are used as stabilizers, a mixed solution composed of distilled water, gelatin molecules and a crosslinking agent is used as a continuous phase, n-hexane is used as a dispersed phase, a Pickering emulsion ink is obtained after homogenization emulsification, a porous scaffold material is obtained through a direct ink writing technology of 3D printing, the porous scaffold material is soaked in salt solutions with different concentrations to improve mechanical properties, the dispersed phase is removed, and a hierarchical interconnected porous scaffold is obtained through freeze-drying.The 3D printing Pickering emulsion ink constructed by the present application has excellent rheological properties, can stably flow through a printer nozzle and allow an extruded filament to be immediately deposited.The printed product is stable, has a variety of channel structures, has good biocompatibility, and can be applied to the field of promoting bone repair in bone tissue engineering.

The invention relates to the technical field of biomedical materials, in particular to a preparation method and application of a photoresponse removable biological scaffold. The preparation method comprises the following steps: by taking double-bond functionalized polycaprolactone at two ends and pentaerythritol tetra-3-mercaptopropionate as raw materials, firstly constructing a basic polymer network through a cross-linking reaction, and then doping BPN-BBTD photo-thermal molecules into the network, so as to obtain the photo-responsive shape memory polymer network. The photoresponse shape memory polymer network can be used for preparing an intravascular stent, and by means of the photoresponse shape memory characteristic of the photoresponse shape memory polymer network, the function that the intravascular stent is shrunk according to needs after being expanded so as to complete stent removal can be achieved; meanwhile, compared with a traditional degradable biological stent, the intravascular stent prepared by the invention can effectively overcome the technical defects of non-uniform and incomplete degradation of the traditional stent, has excellent biomedical safety and clinical application value, and has wide clinical transformation prospects.

The invention provides a casein source 3D printing bio-ink and a preparation method of a biological scaffold and cell culture meat. The bio-ink is prepared from the following raw materials in parts by weight: 15.6 parts of gelatin; 2.6 parts of sodium alginate; 4.2 parts of casein; 52.5 parts of a sodium hydroxide deionized water solution with a pH value of 8; and 87.5 parts of a sterile 1 * PBS buffer solution. The ink is subjected to extrusion type 3D printing and calcium chloride crosslinking to obtain a biological scaffold; c2C12 cells are inoculated on the support, and cell culture meat is obtained through proliferation-differentiation culture. The problems that existing 3D printing bio-ink is single in performance and poor in component adaptability, a biological scaffold material is insufficient in mechanical stability, and nutritional ingredients are deficient are solved. The prepared bio-ink has excellent mechanical properties and printing adaptability, and provides key technical support for industrial preparation of cell culture meat.

The invention relates to a biological 3D printing path overlapping area material extrusion optimization method and system, and the method comprises the steps: obtaining a path planning G code of a 3D printer, extracting biological scaffold paths and endpoint information thereof based on the G code, and calculating a motion equation of each biological scaffold path based on the endpoint information; carrying out pairwise random combination on all biological scaffold paths, constructing a motion equation corresponding to each pair of path combination into a motion equation set, solving the motion equation set, and constructing a coincident point set based on a solving result; generating a control signal based on the coincidence point set, controlling an electric proportional valve to reduce output air pressure based on the control signal, and reducing extrusion of the biological material; the system provided by the invention is used for implementing the method. Compared with the prior art, the method capable of dynamically adjusting the air pressure of the electric proportional valve in the 3D printer is provided, the problem of material stacking in a path overlapping area caused by fixed air pressure is avoided, and therefore the printing precision and efficiency of the biological scaffold are improved.

The invention provides a biological scaffold with a piezoelectric effect as well as a preparation method and application of the biological scaffold. According to the invention, bismuth titanate (Bi4Ti3O12) nanosheets with excellent piezoelectric properties are compounded with poly-L-lactic acid (PLLA) with better biocompatibility through an electrostatic spinning technology for the first time, a novel bionic nervous tissue engineering scaffold is developed, the scaffold has degradability and excellent biocompatibility and safety, the flexibility of the scaffold is improved, and the scaffold can be applied to the field of biological engineering. The defects of high inherent hardness, high brittleness, non-degradability and cytotoxicity of an inorganic piezoelectric material are overcome. The PLLA / Bi4Ti3O12 composite scaffold provided by the invention can significantly promote neuronal differentiation of neural stem cells, inhibits directional differentiation of glial cell lineages while enhancing neuronal differentiation, can be used as an advanced nerve regeneration interface in nervous tissue engineering, provides a very potential strategy for neural circuit reconstruction and functional recovery, and has a wide application prospect. And a new direction is developed for neural restoration and treatment of neurodegenerative diseases.

The invention relates to the technical field of biological scaffolds, in particular to a silk fibroin 4D intelligent hydrogel biological scaffold and a preparation method thereof.The silk fibroin 4D intelligent hydrogel biological scaffold is prepared by taking silk fibroin as a main component and combining with genipin as a cross-linking agent through a printing technology, and the genipin and the silk fibroin form a temperature-sensitive physical cross-linked network; the prepared biological scaffold has the 4D intelligent characteristic and is cured into a temporary shape on a low-temperature platform, and when the hydrogel biological scaffold is implanted into a body (37 DEG C), under stimulation of body temperature, the hydrogel biological scaffold can spontaneously change so as to adapt to and be tightly attached to a specific tissue defect part, so that the tissue defect part is prevented from being damaged. The technical problem that the matching degree of a traditional static stent and a defect part is poor is solved, personalized customization can be performed on damaged tissues of different patients, and the stent has good biocompatibility, excellent mechanical strength, biodegradability and intelligent response characteristics, can promote cell adhesion, proliferation and tissue regeneration, and is particularly suitable for soft tissue repair.

The application discloses a facial filling and lip sculpting method based on a pre-expanding autologous fat pad principle, belongs to the technical field of medical cosmetology and biological tissue engineering, and comprises the following steps: preparing a biological scaffold with a vascularization induction function, and the preparation raw material of the biological scaffold comprises the following components in parts by weight: 80-100 parts of polycaprolactone, 20-30 parts of gelatin, 5-10 parts of dopamine hydrochloride, 2-4 parts of deferoxamine, 3-6 parts of a zwitterionic polymer, 1-3 parts of an activator, 1000-1500 parts of an organic solvent and 500-800 parts of a tris-hydroxymethyl aminomethane-hydrochloric acid buffer solution. The thin film and high porosity are combined, so that the newly generated blood vessels can smoothly penetrate the scaffold and go deep into the interior, the blood supply dead angle in the central region is eliminated, and the probability of fat liquefaction and necrosis is reduced.

The invention relates to application of a casing-source protein-based biological material in tissue engineering, and belongs to the technical field of high-value utilization of biological materials. A protein-based biological material is freeze-dried to obtain gel powder, and the gel powder can be directly mixed with a cell suspension for culture, can be directly used as a surgical suture spinning raw material, and can also be doped with other materials to prepare a biological scaffold; the preparation process of the protein-based biological material comprises the following steps: rehydrating, cleaning, freeze-drying and crushing the edible animal casing; under the non-denaturation condition, active protein components are extracted from the smashed animal casings through an enzyme method or an acid method, and the hydrolysis temperature is avoided in the whole extraction process; and adjusting the pH value of the obtained protein component to 7-8 to obtain the protein-based biological material capable of self-assembling under physiological conditions. Compared with the traditional animal-derived collagen, the collagen provided by the invention has the advantages of safe material source, low cost and small difference among batches, and can meet the diversified requirements of different tissue engineering applications on material performance and form.

The present disclosure relates to compositions, apparatus and methods for generating one or more scaffolds, including: mixing a hydrogel material and / or an extracellular matrix (ECM) protein in an aqueous solvent to generate an aqueous process solution; and cryoelectrospinning the aqueous process solution onto a plurality of conductive probes extending from a conductive surface of a collector plate disposed within a process chamber under conditions sufficient to generate one or more scaffolds configured to mimic a preselected soft tissue decellularized extracellular matrix. Scaffold compositions are also provided having preselected or tuned characteristics. The scaffolds provide a promising stromal cell delivery vehicle for the remediation of fibrosis.

Polyurethane-based tissue fillers useful for treating and / or augmenting tissue, as well as acting as a biological scaffold that promotes cell in-ingrowth and tissue integration, are disclosed, as are quick-setting, injectable precursors of such tissue fillers. Such tissue fillers generally comprise (1) a polyurethane and (2) a particulate acellular tissue matrix. Also disclosed are methods of treating and / or augmenting tissues using such tissue fillers, particularly voids in human tissue such as anal fistulae or hernias.

The invention discloses an edible biological scaffold, a preparation method thereof and application of the edible biological scaffold in preparation of cell culture meat, and belongs to the field of biological scaffold materials. The invention provides a biological scaffold prepared by taking glutenin, chitosan and agarose as raw materials. The biological scaffold is subjected to vacuum freeze drying to form a scaffold material with a porous network structure. Experimental results show that the scaffold material has outstanding hardness, toughness, chewiness and swelling rate, can be used as a scaffold for cell culture, and enables cells to proliferate massively and present a spatial three-dimensional growth form. The invention provides a novel scaffold material for producing cell culture meat, and the scaffold material has the advantages of safe raw materials, wide sources, low cost, simple preparation process and the like, and has wide application prospects in the fields of food science, cell agriculture and the like.

The application belongs to the technical field of natural polymer material modification and application, and discloses a 3D biological printing matrix material based on corn alcohol-soluble protein and a preparation method and application thereof, and particularly relates to a corn alcohol-soluble protein and a graft of pluronic F127 and a preparation method and application thereof in 3D biological printing. The material is obtained by the reaction of terminal alkyne pluronic F127 and poly-peptide modified corn alcohol-soluble protein. The application provides a 3D biological printing matrix material based on corn alcohol-soluble protein and pluronic F127, which has excellent biocompatibility, and the biological scaffold material prepared by using the material can realize excellent cell adhesion and proliferation; the low gel concentration solves the human body hazard of high concentration of F127; the temperature-sensitive characteristics make the material be in a flowable sol state at room temperature and be in a gel state at body temperature, and the biological scaffold material based on the material is formed and stably exists in a body temperature environment.

This invention provides a casein-derived 3D printing bio-ink, a bio-scaffold, and a method for preparing cell-cultured meat. The bio-ink is prepared from the following raw materials in parts by weight: gelatin: 15.6 parts; sodium alginate: 2.6 parts; casein: 4.2 parts; sodium hydroxide deionized water solution at pH=8: 52.5 parts; sterile 1×PBS buffer solution: 87.5 parts. Using this ink, a bio-scaffold is obtained through extrusion 3D printing and calcium chloride cross-linking. C2C12 cells are seeded onto the scaffold, and cell-cultured meat is obtained through proliferation-differentiation culture. This invention solves the problems of existing 3D printing bio-inks, such as limited performance, poor component compatibility, insufficient mechanical stability of bio-scaffold materials, and lack of nutritional components. The prepared bio-ink exhibits excellent mechanical properties and printing compatibility, providing key technical support for the industrial-scale preparation of cell-cultured meat.

The invention provides an endometrial regeneration biological scaffold as well as a preparation method and application thereof. The endometrial regeneration biological scaffold comprises a composite base material framework and a functional modification layer, the composite base material frame has a porous structure, and the functional modification layer is loaded on the surface and internal pores of the composite base material frame; the components of the composite base material frame comprise a natural biological material and a synthetic biological material; the natural biological material comprises collagen and / or hyaluronic acid; the synthetic biological material comprises a polylactic acid-glycolic acid copolymer and / or polycaprolactone; the functional modification layer comprises any one or a combination of at least two of VEGF (vascular endothelial growth factor), IGF-1 (insulin-like growth factor) or LL-37. After the endometrial regeneration biological scaffold is implanted for 7-10 days, the thickness of the endometrium is increased to 7 mm or above, the clinical embryo transplantation standard is met, the degradation period is 21-28 days, and the degradation period is completely matched with the IVF hormone period.