Visual biological scaffold with percolate self-pumping discharge function and preparation method of visual biological scaffold

Abstract

The invention provides a visual biological scaffold capable of discharging percolate in a self-pumping manner and a preparation method of the visual biological scaffold. The visual biological scaffold comprises the following components in parts by weight: 5-20 parts of a hydrophobic high polymer material, 80-95 parts of a volatile solvent, 5-10 parts of a hydrophobic component, 3-15 parts of a hydrophilic high polymer material and 0.1-15 parts of an antibacterial agent. A hydrophilic and hydrophobic material is co-spun through electrostatic spinning, the material is endowed with certain hydrophilicity, the attachment and proliferation rate of cells can be increased, wound healing is promoted, a 3D network microchannel is formed, and the channel has a self-pumping drainage effect and can be used as a miniature infusion pump; the antibacterial substance is carried through electrostatic spinning, the antibacterial substance is physically wrapped in the fiber or dispersed in a fiber matrix or adsorbed on the surface of the fiber, and the antibacterial substance has a slow release effect, so that efficient and continuous sterilization is achieved, and wound infection is prevented; the device has the characteristic of visualization, and the wound healing degree can be observed; the electrostatic spinning in-situ forming technology is adopted, and the biological scaffold deposited on the wound has adhesiveness and does not need to be fixed additionally.

Description

Technical Field

[0001] The present invention relates to the technical field of biological scaffolds, and in particular to a visual biological scaffold capable of exudate self-pump discharge and a preparation method thereof. Background Art

[0002] As the largest organ in the human body, the skin undertakes important functions such as physical protection, immune defense, temperature regulation, and sensory conduction. However, skin damage repair has always been a difficult problem in biomedical research, especially in wound repair and regeneration. How to simulate the skin's natural healing mechanism and construct a microenvironment suitable for cell growth and tissue regeneration has become a hot topic in tissue engineering. The wound repair process often faces many challenges such as exudate management, infection control, and healing monitoring. Traditional wound repair methods have certain limitations in addressing these issues.

[0003] Exudate management is crucial in the treatment of chronic wounds, such as diabetic ulcers and burns. If wound exudate is not effectively managed, it can easily accumulate, leading to maceration of surrounding tissues, impairing wound healing, and potentially worsening wound deterioration and causing complications. Traditional wound dressings, such as hydrogel dressings, while capable of absorbing water, often lack directional drainage, making it difficult to effectively manage exudate.

[0004] Infection is also a common and challenging issue during wound repair. For burn patients, the mortality rate after infection is twice that of uninfected patients. Reportedly, 42%-65% of burn patients die from infection, making infection the leading cause of death after burns. Traditional antimicrobial dressings, such as silver ion dressings, suffer from cytotoxicity and rapid silver ion release, making it difficult to maintain a long-lasting antimicrobial effect. Natural antimicrobial agents, such as chitosan, while biocompatible, have a short antimicrobial effect.

[0005] When it comes to wound healing monitoring, timely and accurate understanding of wound healing status is crucial for adjusting treatment plans. However, most existing wound repair materials cannot meet the needs of real-time, visual observation of wounds. Doctors can often only observe by periodically removing the dressing, which not only increases the risk of wound infection but also makes it difficult to obtain dynamic information on wound healing. In addition, if medical staff are unable to accurately judge the time and treatment method for wound dressing changes, frequent and unnecessary dressing changes may occur, thereby increasing the irritation and pain caused by frequent dressing changes to the wound, and reducing the patient's medical experience.

[0006] The emergence of biological tissue engineering scaffolds has brought new hope for wound repair. As an emerging material for wound repair, skin scaffolds show promising development prospects. By constructing a three-dimensional porous structure, biological tissue engineering scaffolds can support cell growth, mimic the extracellular matrix environment, and promote tissue regeneration. Furthermore, if scaffolds possess exudate drainage, antibacterial properties, and transparent wound visualization capabilities, they are expected to significantly improve wound repair efficiency and reduce complications. However, while currently available skin repair scaffolds have achieved some success in promoting skin repair, they still fail to fully address key issues in the actual repair process. Firstly, their ability to prevent and control bacterial infection is insufficient, making it difficult to effectively reduce the risk of infection during skin scaffold use and inhibit further bacterial growth in the wound. Secondly, their ability to manage exudate is limited. When wounds are exposed to high levels of exudate, they quickly reach saturation, leading to accumulation of exudate around the wound surface and increasing the risk of infection. Finally, some scaffolds lack comfort and conformability, making them unable to provide stable and reliable support and a perfect fit for complex wounds, thus compromising the repair effect. Furthermore, the complex manufacturing processes and high production costs of some scaffolds limit their large-scale clinical application.

[0007] In this context, the development of a new type of biological tissue engineering scaffold that can effectively drain exudate, has long-lasting antibacterial properties, and allows transparent visualization of wounds has important practical significance and clinical application value. This is also a key issue that needs to be urgently addressed in the current field of wound repair. Summary of the Invention

[0008] The purpose of the present invention is to disclose a visual bio-scaffold with self-pump discharge of exudate. By electrospinning and co-spinning hydrophilic and hydrophobic materials, the materials are given a certain hydrophilicity, which can improve the attachment and proliferation rate of cells, promote wound healing, and form a 3D network microchannel. This channel has the function of self-pump drainage and can be used as a micro-infusion pump to help the wound to accelerate the discharge of excess exudate while keeping the wound moist, providing a healing microenvironment for the wound; forming a protective barrier to provide a material exchange place for the wound, with excellent air permeability, allowing drugs to penetrate into the skin, delivering drugs to the wound, and changing the dressing outside the membrane, which protects the new tissue of the wound to the greatest extent, does not hinder the passage of exudate, and can block bacteria, providing a suitable material exchange place for the wound, and accelerating wound healing; carrying antibacterial substances through electrospinning, antibacterial The substance is physically wrapped inside the fiber or dispersed in the fiber matrix or adsorbed on the fiber surface, and has a sustained-release effect, giving it high-efficiency and continuous sterilization to prevent wound infection; it has the characteristic of visualization, which can observe the degree of wound healing. Medical staff can accurately judge the wound exudate and infection, choose appropriate medication and replacement time, and guide medical staff to formulate personalized treatment plans, avoiding unnecessary dressing changes, thereby reducing the irritation and pain caused to the patient's wound due to frequent dressing changes, reducing the patient's pain, and improving the patient's medical experience; using electrospinning in situ forming technology, the biological scaffold deposited on the wound is adhesive and does not require additional fixation. At the same time, it has excellent comfort and compliance, is suitable for various complex wounds, meets the patient's customized needs, and can move freely.

[0009] To achieve the above objectives, the present invention provides a visualized bioscaffold with exudate self-pump discharge, comprising the following components in parts by weight: 5-20 parts of a hydrophobic polymer material, 80-95 parts of a volatile solvent, 5-10 parts of a hydrophobic component, 3-15 parts of a hydrophilic polymer material, and 0.1-15 parts of an antibacterial agent.

[0010] In some embodiments, the hydrophobic polymer material includes one or more combinations of polyvinyl butyral, polyurethane, polyvinyl formal, polytetrafluoroethylene, polycaprolactone, and polylactic acid.

[0011] In some embodiments, the volatile solvent includes one or more combinations of ethanol, dichloromethane, chloroform, acetone, N,N-dimethylformamide, tetrahydrofuran, hexafluoroisopropanol, and trifluoroethanol.

[0012] In some embodiments, the hydrophobic component includes one or more combinations of polydimethylsiloxane, beeswax, cod liver oil, and silicone rubber.

[0013] In some embodiments, the hydrophilic polymer material includes one or more combinations of polyethylene glycol-400, polyethylene glycol-600, polyethylene glycol-1000, polyethylene glycol-1500, polyethylene glycol-4000, and polyethylene glycol-6000.

[0014] In some embodiments, the antibacterial agent comprises one or more combinations of nanosilver, silver nitrate, silver sulfate, silver chloride, sodium silver zirconium phosphate, and silver sulfadiazine.

[0015] To achieve the above object, the present invention further provides a method for preparing a bio-scaffold with a visualizable exudate self-pump discharge, comprising the following steps:

[0016] Step 1: Weigh a volatile solvent into a beaker, slowly add the hydrophobic polymer material into the volatile solvent in small amounts and multiple times, and stir magnetically at room temperature for 2-4 hours until the solution is optically transparent, free of particulate impurities, and forms a stable homogeneous system;

[0017] Step 2: Weigh the hydrophobic component and slowly add it to the solution. Move the mixed solution to a high shear disperser to disperse it, ensuring that the hydrophobic component is evenly dispersed at the micron level without agglomeration or precipitation.

[0018] Step 3: Weigh the hydrophilic polymer material and the antibacterial agent, add them to the mixed solution in sequence, and fix the beaker in a high shear disperser again to disperse at room temperature. The hydrophilic polymer material is dissolved by intermolecular forces, and the antibacterial agent is evenly dispersed under high shear force to obtain a spinning solution;

[0019] Step 4: Transfer the spinning solution to the liquid storage container of the spinning device and precisely control the process parameters: the high-voltage power supply output voltage is 6-20kV, so that the solution forms a Taylor cone and is stretched and refined; a high-precision injection pump extrude the spinning solution at a flow rate of 3-5mL / h; the distance between the receiving device and the spinneret is maintained at 10-12cm to ensure that the jet is fully solidified. Under the synergistic effect of various parameters, a visual bioscaffold with controllable porosity, fiber diameter and mechanical properties is produced.

[0020] In some embodiments, in step 1, during stirring, a glass rod is used to assist in dispersing the material on the inner wall of the beaker.

[0021] In some embodiments, in step 2, the dispersion is performed in an intermittent mode, with a pause every 10 minutes, and the dispersion of the hydrophobic component is observed using an optical microscope at a magnification of 100-400 times.

[0022] Compared with the prior art, the present invention has the following beneficial effects:

[0023] 1. By electrospinning hydrophilic and hydrophobic materials, the materials are given a certain degree of hydrophilicity, which can increase the attachment and proliferation rate of cells, promote wound healing, and form a 3D network microchannel. This channel has the function of self-pump drainage and can be used as a micro-infusion pump to help the wound surface accelerate the discharge of excess exudate while keeping the wound surface moist, providing a healing microenvironment for the wound surface with excellent breathability; allowing drugs to penetrate into the skin and deliver drugs to the wound surface. Dressing changes are all done outside the membrane, protecting the new tissue of the wound surface to the greatest extent; it can prevent bacteria without hindering the passage of exudate, providing a suitable place for material exchange for the wound surface, and accelerating wound healing;

[0024] Second, antibacterial substances are carried by electrospinning. The antibacterial substances are physically wrapped inside the fiber, dispersed in the fiber matrix, or adsorbed on the fiber surface, and have a sustained release effect, giving them high efficiency and continuous sterilization to prevent wound infection;

[0025] 3. With its visual features, the degree of wound healing can be observed. Medical staff can accurately judge the wound exudate and infection, choose appropriate medication and change time, and guide medical staff in formulating personalized treatment plans, avoiding unnecessary dressing changes, thereby reducing the irritation and pain caused by frequent dressing changes to the patient's wound, reducing the patient's suffering, and improving the patient's medical experience;

[0026] Fourth, using electrospinning in-situ forming technology, the biological scaffold deposited on the wound has adhesiveness and does not require additional fixation. At the same time, it has excellent comfort and compliance, is suitable for various complex wounds, meets the patient's customized needs, and can move freely. BRIEF DESCRIPTION OF THE DRAWINGS

[0027] Figure 1 This is a schematic diagram of the structure of the visualized bio-scaffold shown in the present invention;

[0028] Figure 2 This is a functional schematic diagram of the visualized bioscaffold shown in the present invention;

[0029] Figure 3 This is a diagram showing the transparent visualization effect and drainage capability of the visualized biological scaffold of the present invention;

[0030] Figure 4 A water vapor permeability chart of the visualized bioscaffold shown in the present invention;

[0031] Figure 5 This is a scanning electron microscope image of the visualized bioscaffold shown in the present invention. DETAILED DESCRIPTION

[0032] The present invention is described in detail below with reference to the various embodiments shown in the accompanying drawings, but it should be noted that these embodiments are not limitations of the present invention, and any equivalent transformations or substitutions in functions, methods, or structures made by ordinary technicians in this field based on these embodiments are all within the scope of protection of the present invention.

[0033] Example 1:

[0034] This embodiment discloses a visual bioscaffold with exudate self-pump discharge and a preparation method thereof, which specifically includes the following steps:

[0035] 1. Preparation of Hydrophobic Polymer Homogeneous Solution

[0036] Use polyvinyl butyral as the hydrophobic polymer and ethanol as the volatile solvent. Accurately measure 10 parts polyvinyl butyral and 90 parts ethanol. Slowly add the polyvinyl butyral to the ethanol in small, repeated additions. Place in a magnetic stirring chamber and stir at room temperature. Use a glass rod to disperse the material within the beaker. Depending on the material properties and solution concentration, stir for 2-4 hours until the solution is optically transparent, free of particulate impurities, and forms a stable homogeneous system.

[0037] 2. Coordinated dispersion control of hydrophobic components

[0038] After confirming that the hydrophobic polymer has fully dissolved, resulting in a homogeneous solution, accurately weigh 5 parts polydimethylsiloxane and slowly add it to the solution. Transfer the mixed solution to a high-shear disperser for dispersion. Dispersion should be performed in an intermittent mode, pausing every 10 minutes. Observe the solution under an optical microscope at 100-400x magnification to ensure it is a uniform, flowable liquid with no particles or lumps. Ensure that the hydrophobic component is uniformly dispersed at the micron level, without agglomeration or precipitation. If any abnormalities are observed, extend the dispersion time or increase the speed appropriately. However, avoid exceeding the solution temperature of 40°C, which may affect performance.

[0039] 3. Blending of amphiphilic functional components and dispersion of antimicrobial agents

[0040] Using an analytical balance, accurately weigh 3 parts polyethylene glycol-400 and 5 parts nanosilver. Add these to the solution in sequence, then secure the beaker to a high-shear disperser and disperse at room temperature. During this process, the hydrophilic polymer dissolves through intermolecular forces, while the antimicrobial agent disperses evenly under the high shear force. The resulting spinning solution exhibits excellent fluidity and uniformity, meeting the requirements for electrospinning.

[0041] 4. Electrospinning of Biofiber Scaffolds

[0042] The spinning solution was transferred to the liquid storage container of a custom spinning device, where precise process parameters were controlled: a high-voltage power supply output voltage of 6kV was used to form a Taylor cone and stretch the solution into thin layers; a high-precision syringe pump extruded the spinning solution at a flow rate of 3mL / h; and a 10cm distance between the receiving device and the spinneret was maintained to ensure sufficient jet solidification. The synergistic effect of these parameters resulted in the production of a visual bioscaffold with controllable porosity, fiber diameter, and mechanical properties.

[0043] Example 2:

[0044] This embodiment discloses a visual bioscaffold with exudate self-pump discharge and a preparation method thereof, which specifically includes the following steps:

[0045] 1. Preparation of Hydrophobic Polymer Homogeneous Solution

[0046] Use polyurethane as the hydrophobic polymer and dichloromethane as the volatile solvent. Accurately weigh 15 parts polyurethane and 91 parts dichloromethane. Slowly add the polyurethane to the dichloromethane in small, repeated increments. Place the mixture in a magnetic stirrer and stir at room temperature. Use a glass rod to disperse the material along the beaker's interior. Depending on the material properties and solution concentration, stir for 2-4 hours until the solution is optically transparent, free of particulate impurities, and forms a stable, homogeneous system.

[0047] 2. Coordinated dispersion control of hydrophobic components

[0048] After confirming that the hydrophobic polymer has fully dissolved, resulting in a homogeneous solution, accurately weigh 5 parts beeswax and slowly add it to the solution. Transfer the mixture to a high-shear disperser for dispersion. Dispersion should be performed in an intermittent mode, pausing every 10 minutes. Observe the solution under an optical microscope at 100-400x magnification to ensure it is a uniform, flowable liquid with no particles or lumps. Ensure that the hydrophobic component is uniformly dispersed at the micron level, without agglomeration or precipitation. If any abnormalities are observed, extend the dispersion time or increase the speed appropriately. However, avoid exceeding the solution temperature of 40°C, which may affect performance.

[0049] 3. Blending of amphiphilic functional components and dispersion of antimicrobial agents

[0050] Accurately weigh 5 parts polyethylene glycol 600 and 10 parts nanosilver using an analytical balance. Add these to the solution in sequence, then secure the beaker to a high-shear disperser and disperse at room temperature. During this process, the hydrophilic polymer dissolves through intermolecular forces, while the antimicrobial agent disperses evenly under the high shear force. The resulting spinning solution exhibits excellent fluidity and uniformity, meeting the requirements for electrospinning.

[0051] 4. Electrospinning of Biofiber Scaffolds

[0052] The spinning solution was transferred to the liquid storage container of a custom spinning device, where precise process parameters were controlled: a high-voltage power supply output voltage of 6kV was used to form a Taylor cone and stretch the solution into thin layers; a high-precision syringe pump extruded the spinning solution at a flow rate of 3mL / h; and a 10cm distance between the receiving device and the spinneret was maintained to ensure sufficient jet solidification. The synergistic effect of these parameters resulted in the production of a visual bioscaffold with controllable porosity, fiber diameter, and mechanical properties.

[0053] Example 3:

[0054] This embodiment discloses a visual bioscaffold with exudate self-pump discharge and a preparation method thereof, which specifically includes the following steps:

[0055] 2. Preparation of Hydrophobic Polymer Homogeneous Solution

[0056] Using polylactic acid as the hydrophobic polymer, and trifluoroethanol as the volatile solvent, accurately measure 20 parts polylactic acid and 95 parts trifluoroethanol. Slowly add the polylactic acid to the trifluoroethanol in small, repeated increments. Place the mixture in a magnetic stirrer and stir at room temperature. Use a glass rod to disperse the material along the beaker's interior. Depending on the material properties and solution concentration, stir for 2-4 hours until the solution is optically transparent, free of particulate impurities, and forms a stable, homogeneous system.

[0057] 2. Coordinated dispersion control of hydrophobic components

[0058] After confirming that the hydrophobic polymer has fully dissolved, resulting in a homogeneous solution, accurately weigh 10 parts cod liver oil and slowly add it to the solution. The mixture is then transferred to a high-shear disperser for dispersion. Dispersion is performed in an intermittent mode, pausing every 10 minutes. Observe the solution under an optical microscope at 100-400x magnification to ensure that the solution is free of particles or clumps and presents a uniform, flowable liquid. Ensure that the hydrophobic component is uniformly dispersed at the micron level, without agglomeration or precipitation. If any abnormalities are observed, extend the dispersion time or increase the speed appropriately. However, avoid exceeding the solution temperature of 40°C, which can affect performance.

[0059] 3. Blending of amphiphilic functional components and dispersion of antimicrobial agents

[0060] Accurately weigh 9 parts polyethylene glycol 6000 and 15 parts silver sulfate using an analytical balance. Add these to the solution in that order, then secure the beaker to a high-shear disperser and disperse at room temperature. During this process, the hydrophilic polymer dissolves through intermolecular forces, while the antimicrobial agent is evenly dispersed under the high shear force. The resulting spinning solution exhibits excellent fluidity and uniformity, meeting the requirements for electrospinning.

[0061] 4. Electrospinning of Biofiber Scaffolds

[0062] The spinning solution was transferred to the liquid storage container of a custom spinning device, where precise process parameters were controlled: a high-voltage power supply output voltage of 20kV was used to form a Taylor cone and stretch the solution into thin layers; a high-precision syringe pump extruded the spinning solution at a flow rate of 5mL / h; and a 12cm distance between the receiving device and the spinneret was maintained to ensure sufficient jet solidification. The synergistic effect of these parameters resulted in the production of a visual bioscaffold with controllable porosity, fiber diameter, and mechanical properties.

[0063] like Figure 1 and Figure 2 As shown, electrospinning is used to co-spin hydrophilic and hydrophobic materials to form a composite wettability fiber scaffold, and a three-dimensional network microchannel is constructed to achieve self-pump drainage and wound protection; the microchannel has selective permeability, and has the functions of breathability, drug conduction, and antibacterial; antibacterial substances are carried by electrospinning and released slowly, which can kill bacteria and prevent infection in a long time; it has visualization characteristics, which helps medical staff to make accurate judgments and formulate personalized treatment plans; the electrospinning in situ forming technology is used to make the scaffold adhesive and highly comfortable and compliant, which is suitable for complex wounds and can be customized, and comprehensively improves the wound repair effect and patient experience.

[0064] like Figure 3 As shown in the figure, the addition of polyethylene glycol (PEG) resulted in a certain degree of visualization and structured channel drainage. With increasing PEG content, the scaffold's transparency improved, but its self-pumping drainage capability first increased and then weakened. Furthermore, the drainage capability of the scaffold also weakened when the in situ electrospinning time was prolonged.

[0065] like Figure 4 As shown in the figure, the water vapor transmission rate data of the dressings show that the air permeability of each group is good. Figure 5 As shown, the dressing has a large number of pores, further demonstrating good breathability. The fiber filaments successfully carried the antimicrobial agent, which was stably attached to the fiber filaments. Furthermore, as the amount of antimicrobial agent added increased, the fiber filaments carried more nanoparticles. The antimicrobial agent was added at 5 parts (a), 10 parts (b), and 15 parts (c).

[0066] The visualized bioscaffold created by the present invention is a hydrophilic and hydrophobic composite wettability fiber scaffold created by electrospinning co-spinning hydrophilic and hydrophobic materials to give the materials a certain hydrophilicity. At the same time, the introduction of hydrophilic substances into hydrophobic fibers can increase the attachment and proliferation rate of cells and promote wound healing. A 3D network microchannel is formed by the characteristics of electrospinning technology and composite wettability. This channel has the function of self-pump drainage and can be used as a micro-infusion pump to help the wound accelerate the discharge of excess exudate while keeping the wound moist, providing a healing microenvironment for the wound. When the wound exudate is large, highly absorbent materials such as gauze, medical sponges, etc. can be added to the outside of the scaffold to absorb the exudate more quickly. In addition, this channel also has selective permeability, and its structure is similar to the epidermis of the human body, providing a protective barrier for the wound. The stent allows gas exchange between the wound and the external environment and has excellent air permeability; it allows drugs to penetrate the skin and deliver drugs to the wound, and dressing changes are all done outside the membrane, protecting the new tissue of the wound to the greatest extent; it can block bacteria without hindering the passage of exudate, providing a suitable place for material exchange for the wound and accelerating wound healing.

[0067] The visualized bioscaffold created by the present invention carries antibacterial substances through electrospinning. The antibacterial substances are physically wrapped inside the fibers, dispersed in the fiber matrix, or adsorbed on the fiber surface, and have a sustained release effect, giving them high-efficiency and continuous sterilization to prevent wound infection.

[0068] Electrospun fibers typically range in diameter from tens of nanometers to micrometers, possessing extremely high specific surface area and abundant pores, providing numerous loading sites for active substances. Initially, some active substances adsorbed on the fiber surface or in the pores are rapidly released, followed by slow diffusion and release of internal substances through the pores of the fiber matrix or the gaps between polymer segments. Furthermore, the hydrophilicity of the fibers causes structural changes in the presence of exudate or wound surface temperature, such as swelling and precipitation of hydrophilic substances, triggering the controlled release of active substances, achieving a sustained-release effect.

[0069] The visualized bioscaffold created by this invention offers visualization capabilities, allowing for observation of wound healing. Using this dressing, medical staff can accurately assess wound exudate and infection levels, select appropriate medications and change schedules, and provide guidance on developing personalized treatment plans. This avoids unnecessary dressing changes, thereby reducing irritation and pain caused by frequent dressing changes, alleviating patient suffering and enhancing the patient's medical experience. Based on the information provided by the visualized dressing, medical staff can make quick decisions, reducing unnecessary examination and analysis time and improving medical efficiency.

[0070] Different from traditional dressings, this invention adopts electrospinning in-situ forming technology. The biological scaffold deposited on the wound is adhesive and does not require additional fixation. It also has excellent comfort and compliance, is suitable for various complex wounds, meets the patient's customized needs, and can move freely.

[0071] The series of detailed descriptions listed above are only specific descriptions of feasible implementation methods of the present invention. They are not intended to limit the scope of protection of the present invention. Any equivalent implementation methods or changes that do not deviate from the technical spirit of the present invention should be included in the scope of protection of the present invention.

[0072] In addition, it should be understood that although this specification is described in terms of implementation methods, not every implementation method contains only one independent technical solution. This narrative method of the specification is only for the sake of clarity. Those skilled in the art should regard the specification as a whole. The technical solutions in each embodiment can also be appropriately combined to form other implementation methods that can be understood by those skilled in the art.

Claims

1. A bio-scaffold with a visualizable pump-type exudate discharge, characterized in that: The composition comprises the following components in parts by weight: 5-20 parts of hydrophobic polymer material, 80-95 parts of volatile solvent, 5-10 parts of hydrophobic component, 3-15 parts of hydrophilic polymer material and 0.1-15 parts of antibacterial agent.

2. The visualized bioscaffold with exudate self-pump discharge according to claim 1, characterized in that: The hydrophobic polymer material includes one or more combinations of polyvinyl butyral, polyurethane, polyvinyl formal, polytetrafluoroethylene, polycaprolactone, and polylactic acid.

3. The bio-scaffold with visualization function and pump-type exudate discharge according to claim 1, characterized in that: The volatile solvent includes one or more combinations of ethanol, dichloromethane, chloroform, acetone, N,N-dimethylformamide, tetrahydrofuran, hexafluoroisopropanol, and trifluoroethanol.

4. The bio-scaffold with visualization function and pump-type exudate discharge according to claim 1, characterized in that: The hydrophobic component includes one or more combinations of polydimethylsiloxane, beeswax, cod liver oil, and silicone rubber.

5. The visualized bioscaffold with exudate self-pump discharge according to claim 1, characterized in that: The hydrophilic polymer material includes one or more combinations of polyethylene glycol-400, polyethylene glycol-600, polyethylene glycol-1000, polyethylene glycol-1500, polyethylene glycol-4000, and polyethylene glycol-6000.

6. The visualized bioscaffold with exudate self-pump discharge according to claim 1, characterized in that: The antibacterial agent includes one or more combinations of nano silver, silver nitrate, silver sulfate, silver chloride, sodium zirconium phosphate, and silver sulfadiazine.

7. A method for preparing a visualized bioscaffold with exudate self-pump discharge according to any one of claims 1 to 6, characterized in that: The steps include: Step 1: Weigh a volatile solvent into a beaker, slowly add the hydrophobic polymer material into the volatile solvent in small amounts and multiple times, and stir magnetically at room temperature for 2-4 hours until the solution is optically transparent, free of particulate impurities, and forms a stable homogeneous system; Step 2: Weigh the hydrophobic component and slowly add it to the solution. Move the mixed solution to a high shear disperser to disperse it, ensuring that the hydrophobic component is evenly dispersed at the micron level without agglomeration or precipitation. Step 3: Weigh the hydrophilic polymer material and the antibacterial agent, add them to the mixed solution in sequence, and fix the beaker in a high shear disperser again to disperse at room temperature. The hydrophilic polymer material is dissolved by intermolecular forces, and the antibacterial agent is evenly dispersed under high shear force to obtain a spinning solution; Step 4: Transfer the spinning solution to the liquid storage container of the spinning device and precisely control the process parameters: the high-voltage power supply output voltage is 6-20kV, so that the solution forms a Taylor cone and is stretched and refined; a high-precision injection pump extrude the spinning solution at a flow rate of 3-5mL / h; the distance between the receiving device and the spinneret is maintained at 10-12cm to ensure that the jet is fully solidified. Under the synergistic effect of various parameters, a visual bioscaffold with controllable porosity, fiber diameter and mechanical properties is produced.

8. The method for preparing a bio-scaffold with visualization of exudate discharge by pump according to claim 7, characterized in that: In step 1, during stirring, a glass rod was used to assist in dispersing the material on the inner wall of the beaker.

9. The method for preparing a bio-scaffold with visualization of exudate discharge by pump according to claim 7, characterized in that: In step 2, the dispersion is performed in an intermittent mode, with a pause every 10 minutes, and the dispersion of the hydrophobic component is observed by optical microscopy at a magnification of 100-400 times.

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