9 results about "Culture bacteria" patented technology

The application concerns a microbiological system supporting the consolidation of sand and sandy soils through biocementation, utilizing a mixture of bacteria from the Sporosarcina genus, including Sporosarcina sp. ANT_H38, deposited in the Polish Collection of Microorganisms (PCM) at the Institute of Immunology and Experimental Therapy of the Polish Academy of Sciences in Wrocław under number B / 00547, and Sporosarcina pasteurii DSM 33. The goal is to stabilize and strengthen soil structures through natural mineralization, which increases the substrate's load-bearing capacity. The process involves culturing the bacteria on LB medium, diluting them and combining them with urea, and then applying them to sand or soil with the addition of a cementing medium. The system also operates effectively at low temperatures, enabling broad applications in construction and environmental protection. The application also concerns a method for producing the microbiological system and a method for supporting the consolidation process of sand and / or sandy soils using this system.

Provided herein are multi-strain population control systems, methods, kits, and compositions. Also provided are methods, systems, kits, and compositions for culturing bacterial cells in multi-strain ecosystems, and temporally arranged multi-strain ecosystems or cultures using a synchronized lysis circuit in combination with multiple toxin / antitoxin systems to cycle continuously over a long period of time.

This invention enables accurate measurement of greenhouse gas emissions from animals. It also allows for easy evaluation of the reduction effect of greenhouse gas emissions from animals. Furthermore, it provides a methane emission inhibitor that suppresses methane production from Methanobrevibacter bacteria. [Solution] A bacterial quantification method for quantifying Methanobrevibacter species contained in the rectal feces of animals includes the steps of: collecting rectal feces from an animal; holding the rectal fecal sample in an anaerobic porter under anaerobic conditions; replacing the gas in the anaerobic porter with nitrogen gas, diluting the sample with a culture medium, and culturing the bacteria in a test tube; recovering the cultured bacteria; extracting DNA from the recovered bacteria; and quantifying the Methanobrevibacter species from the extracted DNA by quantitative PCR or a gene analysis method using next-generation sequencing analysis.

The invention relates to biotechnology, namely to a process for cultivating theBacillus velezensisbacterial strain, which can be used in the field of medicine for producing protein preparations, a source of bioactive peptides.The process for cultivating theBacillus velezensisCNMN-BB-12 bacterial strain, according to the invention, includes inoculating the bacterial suspension in a concentration of (2.0±0.2)·102CFU / mL in a medium containing, g / L: peptone 19.5, soluble starch 1.5 and distilled water the rest, or casein peptone 10.0, yeast extract 5.0, NaCl 10.0 and distilled water the rest, and cultivating on a rotary shaker at a speed of 200 rpm, for 48 hours, at a temperature of 30±1°C, with subsequent separation of the biomass from the culture liquid by centrifugation at 6000 rpm, for 20 min.The result of the invention consists in increasing the content of synthesized proteins in the culture liquid by 3.69-6.90 times.

This invention relates to the field of gene technology, specifically disclosing a method for enhancing the secretion of secondary metabolites based on the regulation of quorum sensing signal molecules. This method activates the expression of bacterial secondary metabolite synthesis gene clusters through bacterial-fungal interaction, comprising the following steps: (a) co-culturing bacteria and fungi in contact or non-contact mode; (b) during co-culturing, the fungi secrete the quorum sensing signal molecule farnesol; (c) the bacteria sense farnesol through a two-component system, activating the expression of secondary metabolite synthesis genes. This invention effectively overcomes the gene silencing problem caused by metabolic load or lack of regulation in natural strains by activating the originally trace expression of secondary metabolite synthesis gene clusters in bacteria using the fungal-derived quorum sensing signal molecule farnesol.

The present application relates to the technical field of mineral processing, and particularly relates to a desulfurization smelting process for high-sulfur tin concentrate, comprising the following steps: ore pretreatment, tin ore is crushed, ground and screened to obtain ore powder; bacteria cultivation and inoculation, bacteria suitable for high-sulfur environment are selected and inoculated into a small-scale cultivation tank and cultivated in a sterile environment; leaching reaction, the pretreated ore powder is mixed with water, bacterial culture solution is added to prepare ore slurry, and the ore slurry is leached; solution treatment and tin recovery, tin metal is recovered after solid-liquid separation of the ore slurry, and waste liquid is treated.The present application provides a desulfurization smelting process for high-sulfur tin concentrate, effectively solves the problems of environmental pollution and high cost existing in the traditional smelting process through the bacterial leaching method, bacteria can grow and reproduce quickly in a high-sulfur environment, so that the desulfurization efficiency is improved, the process operation is simple, low in cost and friendly to the environment, and the tin recovery rate can be significantly improved.

This invention belongs to the field of microbial culture technology. It provides a method for culturing cariogenic acid-producing anaerobic bacteria and its application. The culture medium comprises the following components in the following proportions: peptone 0.5-5%, sucrose 1-10%, sodium chloride 0.1-0.6%, bromocresol green 0.1-0.3%, bromocresol purple 0.01-0.15%, vitamin C 0.01-0.05%, Tween-80 0.01-0.06%, phenylethanol 0.01-0.25%, and deionized water, forming a liquid culture medium. Based on the specific requirements of bacterial culture, this invention is used for culturing bacteria from teeth and the oral cavity. Peptone is used as the nitrogen source, and sucrose as the carbon source. When bacteria multiply rapidly, they convert sucrose into an acidic substance, changing the pH of the culture medium. In the presence of bromocresol green / bromocresol purple, the color of the culture medium changes, allowing for the determination of bacterial content in the sample and the prediction of the risk of dental caries.

The utility model relates to photosynthetic bacteria group culture equipment technical field discloses photosynthetic bacteria group expansion culture device, including culture jar, the outer wall bottom of culture jar is fixedly connected with expansion mechanism, the expansion mechanism is used for expanding culture bacteria group, the inner wall middle part of expansion mechanism is rotatably connected with feeding mechanism, the feeding mechanism is used for stirring bacteria group, the outer wall top of culture jar is fixedly connected with control mechanism, the expansion mechanism includes expansion jar, the expansion jar fixedly connected in the outer wall bottom of culture jar, the inner wall top of expansion jar is fixedly connected with the connecting cover, the outer wall top of connecting cover is equipped with the feeding hole, in the utility model, the bacteria group growth in culture jar, need to expand the culture when entering expansion jar through the feeding hole of connecting cover, and connecting frame fixed support supplies light, and a large number of supply mode real -time response bacteria group metabolic change, improve photosynthetic bacteria group culture's stability and product yield.

This invention enables accurate measurement of greenhouse gas emissions from animals. It also allows for easy evaluation of the reduction effect of greenhouse gas emissions from animals. Furthermore, it provides a methane emission inhibitor that suppresses methane production from Methanobrevibacter bacteria. [Solution] A bacterial quantification method for quantifying Methanobrevibacter species contained in the rectal feces of animals includes the steps of: collecting rectal feces from an animal; holding the rectal fecal sample in an anaerobic porter under anaerobic conditions; replacing the gas in the anaerobic porter with nitrogen gas, diluting the sample with a culture medium, and culturing the bacteria in a test tube; recovering the cultured bacteria; extracting DNA from the recovered bacteria; and quantifying the Methanobrevibacter species from the extracted DNA by quantitative PCR or a gene analysis method using next-generation sequencing analysis.