65 results about "Culture bacteria" patented technology

The utility model provides a culture bottle with physical isolation, which comprises a culture bottle body and an embedded structure, the culture bottle body is used for containing nutrient solution, the culture bottle body is provided with a bottle opening, and the bottle opening is provided with a bottle opening plug for sealing the culture bottle body; the embedded structure is positioned in the culture bottle body and is provided with a containing part for containing adsorbates and an anti-leakage part for preventing the adsorbates from leaking. Compared with the prior art, the internal structure of the culture bottle is optimized, so that the nutrient solution and the resin in the culture bottle are physically isolated, and the problem that the bacterium culture capacity of the culture bottle is reduced due to contact of the nutrient solution and the resin is solved.

Provided a method for promoting growth of bacteria of the genus Bifidobacterium. The method for promoting growth of bacteria of the genus Bifidobacterium includes subjecting a milk culture medium containing one or more selected from the group consisting of cysteine, cystine, and a salt thereof to heat treatment and then culturing bacteria of the genus Bifidobacterium in the milk culture medium.

The invention discloses an optimized recombinant chicken double-cell factor as well as a preparation method and application thereof. The chicken recombinant double-cell factor coding gene provided by the invention contains complete ChIL-2 and ChIFN-alpha structures. The preparation process comprises the following steps: selecting a pichia pastoris expression system; optimizing the recombinant chicken double-cell factor according to the codon preference of pichia pastoris; inserting the optimized gene sequence into an expression vector, and introducing host bacteria to construct a recombinant yeast engineering strain; carrying out inoculated culture by using the obtained recombinant yeast, and screening strains containing target protein based on expression level to obtain required culture bacteria; and breeding and culturing the obtained high-expression-level culture bacteria. According to the invention, ChIL-2 and ChIFN-alpha are connected through a modified flexible linker, and a nucleotide sequence is replaced by preferred codons of pichia pastoris, so that a chicken recombinant cell factor can be secreted to a supernatant, the defect of ChIL-2 expression quantity is overcome, and the problem that proteins of ChIL-2 and ChIFN-alpha mainly exist in bacteria and inclusion bodies in prokaryotic expression is solved.

The utility model relates to the technical field of culture bottles, and discloses a breathable high-productivity culture bottle which comprises a culture bottle body, and a breathable film is sleeved outside a bottle opening of the culture bottle body; the supporting rods are connected to the four corners of the top of the culture bottle body, and assembling grooves are formed in the positions, corresponding to the culture bottle body, of the four corners of the bottom of the culture bottle body; and a bulge is arranged at the bottom of the culture bottle body. The mouth of the culture bottle is covered with the breathing film, oxygen can enter the culture bottle, microorganism pollution and liquid leakage are prevented, breathing of internal culture bacteria is facilitated, multiple groups of culture bottle bodies can be stacked and placed through the supporting rods and the assembling grooves, hot air circulation between the upper portion and the lower portion is guaranteed, heating is uniform, meanwhile, a large amount of oxygen can enter the culture bottle conveniently, and the culture bottle bodies can be placed conveniently. The culture bottle is simple in structure and convenient to use, rapid growth of bacteria is facilitated, classified management is facilitated, multi-group culture efficiency is improved, the protrusions are arranged at the bottom of the culture bottle body, the contact area and the heating area of the bacteria and oxygen are increased, and culture yield is increased.

1. The name of the design product: filter core (different single hole ladder B). 2. The use of the design product: the design product is used in a device for filtering water quality; or used for placing in a fish tank to filter water quality, cultivate bacteria; or used for decoration inside the fish tank. 3. The design points of the design product: in shape. 4. The picture or photo that best shows the design points: perspective view.

The utility model relates to the technical field of liquid storage barrel mounting racks, in particular to a culture bacteria liquid storage barrel mounting rack which comprises a base, supporting legs are arranged at the top of the base, mounting plates are arranged on the outer surfaces of the bottoms of the supporting legs, a transverse rod is arranged on one side of each supporting leg, and mounting blocks are arranged on the two sides of each transverse rod. A first screw is arranged on one side of the mounting block, an adjusting mechanism is arranged on one side of the supporting leg, a connecting plate is arranged on the top of the adjusting mechanism, a sliding groove is formed in the top of the connecting plate, and a clamping mechanism is arranged on the top of the connecting plate. According to the culture bacterium liquid storage barrel mounting frame, the supporting legs and the cross rods are arranged, the mounting frame can be conveniently mounted and dismounted through cooperation of the supporting legs and the cross rods, then the working efficiency can be improved, the clamping mechanisms are arranged, irregular storage barrels can be clamped and fixed through the clamping mechanisms, and then the application range of equipment can be expanded.

1. The name of the design product: aquarium filter material (gear bacteria breeding column). 2. The use of the design product: purification, filtration, adsorption and cultivation of nitrifying bacteria for aquariums. 3. The design points of the design product: in shape. 4. The picture or photo that best indicates the design points: perspective view.

The invention discloses a method for rapidly detecting the composite toxicity of micro-nano plastic and coexisting pollutants, which comprises the following steps of: preparing a working bacterial suspension, namely culturing bacteria and separating and resuspending thalli; poison exposure: sequentially adding the pollutant solution, the micro-nano plastic dispersion liquid and the working bacterium suspension into each hole of a light-proof 96-hole plate to form an exposure solution; detecting the luminous intensity: detecting the luminous intensity of each hole by using a microplate reader at high flux, and calculating the luminous inhibition ratio; and compound toxicity judgment: judging the action type and the action intensity of the compound toxicity by using an independent action model. By combining a Q67 toxicity test and a microwell plate high-throughput detection technology, the action type and the action strength of the composite toxicity are judged by utilizing an independent action model, and the toxicity effects of dozens of composite exposure groups can be synchronously evaluated in a single exposure experiment of 60 minutes, so that the composite pollution risk evaluation efficiency is remarkably improved, and the evaluation cost is reduced.

The present invention provides a method for preparing an antibiotic carrier based on bacterial outer membrane vesicles, characterized in that the preparation method comprises the following steps: (1) culturing bacteria in a culture medium containing antibiotics; (2) removing live bacteria from the culture medium and extracting the bacterial outer membrane vesicles, thereby obtaining an antibiotic carrier based on bacterial outer membrane vesicles; wherein the bacteria is a Salmonella mutant strain QS0074, whose deposit number is CCTCC M 2023192; and the antibiotic is acamycin. The antibiotic carrier based on bacterial outer membrane vesicles provided by the present invention has an excellent drug loading capacity for acamycin and good drug activity stability; the preparation method of the present invention is simple and easy to promote.

1. The name of this design product: filter element (special-shaped single-hole block). 2. Purpose of this design product: The design product is used in a device for filtering water; or is placed in a fish tank to filter water and cultivate bacteria; or is used for decoration inside a fish tank. 3. The key point of the design of this product lies in its shape. 4. The picture or photo that best illustrates the design points: three-dimensional picture.

The application concerns a microbiological system supporting the consolidation of sand and sandy soils through biocementation, utilizing a mixture of bacteria from the Sporosarcina genus, including Sporosarcina sp. ANT_H38, deposited in the Polish Collection of Microorganisms (PCM) at the Institute of Immunology and Experimental Therapy of the Polish Academy of Sciences in Wrocław under number B / 00547, and Sporosarcina pasteurii DSM 33. The goal is to stabilize and strengthen soil structures through natural mineralization, which increases the substrate's load-bearing capacity. The process involves culturing the bacteria on LB medium, diluting them and combining them with urea, and then applying them to sand or soil with the addition of a cementing medium. The system also operates effectively at low temperatures, enabling broad applications in construction and environmental protection. The application also concerns a method for producing the microbiological system and a method for supporting the consolidation process of sand and / or sandy soils using this system.

The invention discloses a method for preparing bacterial cellulose through fermentation by taking degumming waste liquid as a carbon source, which comprises the following steps: degumming waste liquid is detoxified to obtain degumming waste liquid detoxified liquid; and inoculating a bacterial cellulose producing strain into a fermentation culture medium containing the degumming waste liquid detoxification liquid, and carrying out fermentation culture by taking the degumming waste liquid detoxification liquid as a carbon source to obtain the bacterial cellulose. According to the invention, the degumming waste liquid is used as a carbon source for fermentation culture of bacterial cellulose, and the cost is reduced by 35-50% compared with a traditional fermentation preparation method. The stress of the prepared bacterial cellulose gel film is 8-10 M'pa, the strain is 30%-45%, and the bacterial cellulose gel film is particularly suitable for enhancement and modification of polymer-based composite materials, is also suitable for biomedical and intelligent responsive materials and the like, and has a wide application prospect.

Provided herein are multi-strain population control systems, methods, kits, and compositions. Also provided are methods, systems, kits, and compositions for culturing bacterial cells in multi-strain ecosystems, and temporally arranged multi-strain ecosystems or cultures using a synchronized lysis circuit in combination with multiple toxin / antitoxin systems to cycle continuously over a long period of time.

The invention discloses a high-salt pharmaceutical wastewater-based low-cost halotolerant bacterium culture medium and an expanding culture method thereof, and belongs to the field of environmental protection and microbial fermentation. According to the culture medium, 90%-95% of pretreated high-salt pharmaceutical wastewater is used as a substrate and matched with industrial auxiliary materials such as industrial glucose and urea, the salinity of the pretreated wastewater is 20000-30000 mg / L, and the COD is 10000-20000 mg / L. The expanding culture method comprises the steps of strain activation, seed solution preparation, 2 m < 3 > batch scale expanding culture, harvesting and storage, microporous aeration and mechanical stirring are adopted to guarantee uniform dissolved oxygen, and a trehalose-glycerol composite protective agent is added. According to the method, the cost of a ton of fungicide is greatly reduced by using waste culture bacteria, the cell density of the fungicide is greater than or equal to 30,000,000 / mL, the 36h COD removal rate is greater than or equal to 70%, the activity attenuation is less than or equal to 5% after 30 days of refrigeration at 4 DEG C, the problems of high cost, difficulty in large-scale production and poor stability in the prior art are solved, and the method is suitable for biological enhancement treatment of various types of high-salt pharmaceutical wastewater.

A mass production method using Bacillus velezensis for decomposition of fish scales and product of the same is provided. The Bacillus velezensis is deposited in Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures GmbH with an accession number of DSM 34894. The mass production method includes the steps of culturing a bacterial solution of Bacillus velezensis, mixing the bacterial solution with a fish scale material to get a mixed solution, and allowing the mixed solution to react at 37° C. for 12-24 hours for decomposition of the fish scale material. The present mass production method not only can be used to decompose a larger quantity of fish scales but also applied to mass production of a fish scale decomposition product. Thereby the present method has industrial application value.

The invention discloses a method for detecting pseudomonas aeruginosa in a beverage, which comprises the following steps: step 1, mixing and incubating a pseudomonas aeruginosa aptamer solution and a colloidal gold solution, then adding a NaCl solution, uniformly mixing and reacting to obtain a mixed solution; step 2, mixing and incubating the mixed solution and a to-be-detected sample solution, then adding a Tb-MOFs solution, and uniformly mixing and reacting to obtain a to-be-detected precursor solution; and 3, measuring a fluorescence signal of the precursor solution to be measured. According to the method, detection can be realized by using a fluorescence spectrometer, the detection range is 1-106 CFU / mL, and the detection limit is 0.63 CFU / mL. The terbium-based metal organic framework material is used as a fluorescence signal element for the first time, and the aptamer is used as a target recognition element, so that the fluorescent probe has good anti-interference capability and is suitable for solution systems such as water and beverages. In addition, the method does not need strain culture and has the advantages of simplicity and convenience in operation, high sensitivity and the like.

The invention discloses a method for evaluating the antibacterial property of an in-situ prepared material, and belongs to the technical field of antibacterial property testing. The antibacterial performance evaluation method comprises the following steps: preparing a sample: uniformly inoculating a bacterial liquid on the surface of a solid culture medium, enabling the surface of the culture medium inoculated with the bacterial liquid to have a first detection position and a second detection position, and forming a to-be-detected sample in situ at the second detection position; evaluating the antibacterial performance: culturing bacteria by using a culture medium, and observing the growth conditions of the bacteria at the first detection position and the second detection position; compared with the first detection position, if the second detection position forms an antibacterial area, the to-be-detected sample has antibacterial property; if the antibacterial area is not formed at the second detection position, the to-be-detected sample does not have the antibacterial property. The to-be-detected sample with a specific structure is directly formed in situ at the second detection position, so that the to-be-detected sample can be prevented from being damaged when the formed to-be-detected sample is transferred to the culture medium, and the antibacterial property of the to-be-detected sample can be intuitively and accurately evaluated.

1. The name of the design product: filter core (different single hole ladder C). 2. The use of the design product: the design product is used in a device for filtering water quality; or used for placing in a fish tank to filter water quality, cultivate bacteria; or used for decoration inside the fish tank. 3. The design points of the design product: in shape. 4. The picture or photo that best shows the design points: perspective view.

The present invention is directed, inter alia, to compositions comprising a population of at least one strain of bacteria at least partially attached to particles, the particles comprise (i) a polysaccharide-based material; and (ii) a metal carbonate. Uses of the compositions, e.g., for prevention or treatment of dysbiosis, and methods for the preparation thereof are further provided herein.

1. The name of the design product: filter core (different single hole petal ladder A). 2. The use of the design product: the design product is used in a device for filtering water quality; or used for placing in a fish tank to filter water quality, cultivate bacteria; or used for decoration inside the fish tank. 3. The design points of the design product: in shape. 4. The picture or photo that best shows the design points: perspective view.

This invention enables accurate measurement of greenhouse gas emissions from animals. It also allows for easy evaluation of the reduction effect of greenhouse gas emissions from animals. Furthermore, it provides a methane emission inhibitor that suppresses methane production from Methanobrevibacter bacteria. [Solution] A bacterial quantification method for quantifying Methanobrevibacter species contained in the rectal feces of animals includes the steps of: collecting rectal feces from an animal; holding the rectal fecal sample in an anaerobic porter under anaerobic conditions; replacing the gas in the anaerobic porter with nitrogen gas, diluting the sample with a culture medium, and culturing the bacteria in a test tube; recovering the cultured bacteria; extracting DNA from the recovered bacteria; and quantifying the Methanobrevibacter species from the extracted DNA by quantitative PCR or a gene analysis method using next-generation sequencing analysis.

The present disclosure provides a method for quantitatively measuring the adhesion force between bacteria and cells based on fluid force microscopy, comprising: culturing bacteria and cells separately, adding bacteria to the cells to allow the bacteria and cells to interact; selecting a fluid force microscopy probe and pre-treating the probe; searching for a single live bacterium to be captured in a liquid environment where the bacteria and cells interact, applying negative pressure using a fluid force microscopy pressure controller to cause the pinhole at the probe tip to absorb the bacteria, thereby obtaining a fluid force microscopy probe that captures a single bacterium; bringing the fluid force microscopy probe that captures a single bacterium close to the cell surface at a preset speed, staying there for a preset time when a preset force is reached, and then returning to the probe to obtain adhesion force curve data between the bacteria and cells. Utilizing the present disclosure, the degree of interaction between a single bacterium and a cell can be accurately characterized, and the adhesion force between a single bacterium and a cell can be efficiently and quantitatively measured.

The utility model discloses a solid-liquid separation device for culturing strains, which relates to the technical field of solid-liquid separation for culturing strains and comprises a liquid collecting barrel fixed on the ground, a liquid outlet is arranged at the lower end of the liquid collecting barrel, a communicating pipe is rotatably arranged on the liquid collecting barrel in a penetrating manner, and one end of the communicating pipe is connected with a filter component. A driving assembly for driving the communicating pipe to rotate is arranged on the liquid collecting barrel, an input assembly used for being connected with a solid-liquid mixture input pipe is arranged on the liquid collecting barrel, a fixing frame is connected to the liquid collecting barrel, and a solid matter removing structure is arranged on the fixing frame. According to the solid-liquid separation device, the first motor is arranged to drive the separation roller to rotate to perform solid-liquid separation on a solid-liquid mixture, the solid-liquid separation efficiency is improved, solid substances filtered out of the separation roller are continuously cleaned and collected by arranging the solid substance cleaning structure, and the solid-liquid separation efficiency of the separation roller is maintained.

1. The name of the design product: biochemical incubator. 2. The use of the design product: the design product is used for cultivating bacteria, mold, microorganism and breeding test. 3. The design points of the design product: in shape. 4. The picture or photo that can best show the design points: perspective view.

The invention relates to biotechnology, namely to a process for cultivating theBacillus velezensisbacterial strain, which can be used in the field of medicine for producing protein preparations, a source of bioactive peptides.The process for cultivating theBacillus velezensisCNMN-BB-12 bacterial strain, according to the invention, includes inoculating the bacterial suspension in a concentration of (2.0±0.2)·102CFU / mL in a medium containing, g / L: peptone 19.5, soluble starch 1.5 and distilled water the rest, or casein peptone 10.0, yeast extract 5.0, NaCl 10.0 and distilled water the rest, and cultivating on a rotary shaker at a speed of 200 rpm, for 48 hours, at a temperature of 30±1°C, with subsequent separation of the biomass from the culture liquid by centrifugation at 6000 rpm, for 20 min.The result of the invention consists in increasing the content of synthesized proteins in the culture liquid by 3.69-6.90 times.

The application relates to a preparation method of a bacterial cellulose-based multilayer structure functional bionic scaffold, which comprises the following steps: firstly, injecting a biomaterial solution into a silica gel model with a microchannel system to perform freeze drying to obtain a porous biological scaffold; secondly, performing crosslinking treatment on the porous biological scaffold; thirdly, inoculating a culture bacteria solution with a Xylobacter concentration of 10 9 ~ 10 15 individuals / mL on the crosslinking-treated porous biological scaffold, and performing static culture and freeze drying to obtain a bacterial cellulose-based composite scaffold; and finally, adopting a coating solution to coat the bacterial cellulose-based composite scaffold through a microchannel system, and performing demolding treatment to obtain the bacterial cellulose-based multilayer structure functional bionic scaffold. The application provides a functional coating process for the multilayer bionic scaffold by using the shrinkage space of the treated material, the micro-pore channel and the injection method of the patterned drainage network, effectively prevents the penetration of the coating solution, and realizes the perfect solidification of the coating solution on the scaffold.

The utility model relates to the field of strain culture, in particular to an anti-pollution culture medium split charging device which comprises a base plate and a test tube arranged above the base plate, and a driving part is arranged on one side of the base plate; the sterilization assembly is arranged at the driving end of the driving part; a motor of the sterilization assembly is matched with an air pipe, hot air sterilization can be conducted on the inner wall of the test tube while hot air sterilization is conducted on the outer surface of the test tube through the air pipe, bacteria or viruses generated during operation of personnel can be effectively removed, and the culture environment of culture bacteria is guaranteed; by arranging the air bag, when the air pipe blows hot air to the inner wall of the test tube for sterilization and returns, the inner wall of the test tube can be wiped, microbial contamination on the hand of an operator or a tool can be effectively reduced, and therefore the risk of cross contamination is reduced, and it is ensured that the test tube is kept sterile.

1. Name of the Design Product: Filter Core (Irregular Single-hole Octahedron). 2. Use of the Design Product: The design product is used in a water filtration equipment; or it can be placed in an aquarium for water filtration and bacteria cultivation; or it can be used for interior decoration of the aquarium. 3. Design Key Points of the Design Product: Lies in the shape. 4. Picture or Photograph that Best Illustrates the Design Key Points: Perspective view.

The feed production device includes a first water tank in which bacteria and plankton are raised, and a second water tank in which the bacteria and plankton that are raised in the first water tank are annihilated to create a feed for leptocephalus.

This invention relates to the field of gene technology, specifically disclosing a method for enhancing the secretion of secondary metabolites based on the regulation of quorum sensing signal molecules. This method activates the expression of bacterial secondary metabolite synthesis gene clusters through bacterial-fungal interaction, comprising the following steps: (a) co-culturing bacteria and fungi in contact or non-contact mode; (b) during co-culturing, the fungi secrete the quorum sensing signal molecule farnesol; (c) the bacteria sense farnesol through a two-component system, activating the expression of secondary metabolite synthesis genes. This invention effectively overcomes the gene silencing problem caused by metabolic load or lack of regulation in natural strains by activating the originally trace expression of secondary metabolite synthesis gene clusters in bacteria using the fungal-derived quorum sensing signal molecule farnesol.