21 results about "Culture bacteria" patented technology

The application concerns a microbiological system supporting the consolidation of sand and sandy soils through biocementation, utilizing a mixture of bacteria from the Sporosarcina genus, including Sporosarcina sp. ANT_H38, deposited in the Polish Collection of Microorganisms (PCM) at the Institute of Immunology and Experimental Therapy of the Polish Academy of Sciences in Wrocław under number B / 00547, and Sporosarcina pasteurii DSM 33. The goal is to stabilize and strengthen soil structures through natural mineralization, which increases the substrate's load-bearing capacity. The process involves culturing the bacteria on LB medium, diluting them and combining them with urea, and then applying them to sand or soil with the addition of a cementing medium. The system also operates effectively at low temperatures, enabling broad applications in construction and environmental protection. The application also concerns a method for producing the microbiological system and a method for supporting the consolidation process of sand and / or sandy soils using this system.

The invention discloses a method for preparing bacterial cellulose through fermentation by taking degumming waste liquid as a carbon source, which comprises the following steps: degumming waste liquid is detoxified to obtain degumming waste liquid detoxified liquid; and inoculating a bacterial cellulose producing strain into a fermentation culture medium containing the degumming waste liquid detoxification liquid, and carrying out fermentation culture by taking the degumming waste liquid detoxification liquid as a carbon source to obtain the bacterial cellulose. According to the invention, the degumming waste liquid is used as a carbon source for fermentation culture of bacterial cellulose, and the cost is reduced by 35-50% compared with a traditional fermentation preparation method. The stress of the prepared bacterial cellulose gel film is 8-10 M'pa, the strain is 30%-45%, and the bacterial cellulose gel film is particularly suitable for enhancement and modification of polymer-based composite materials, is also suitable for biomedical and intelligent responsive materials and the like, and has a wide application prospect.

Provided herein are multi-strain population control systems, methods, kits, and compositions. Also provided are methods, systems, kits, and compositions for culturing bacterial cells in multi-strain ecosystems, and temporally arranged multi-strain ecosystems or cultures using a synchronized lysis circuit in combination with multiple toxin / antitoxin systems to cycle continuously over a long period of time.

The invention discloses a high-salt pharmaceutical wastewater-based low-cost halotolerant bacterium culture medium and an expanding culture method thereof, and belongs to the field of environmental protection and microbial fermentation. According to the culture medium, 90%-95% of pretreated high-salt pharmaceutical wastewater is used as a substrate and matched with industrial auxiliary materials such as industrial glucose and urea, the salinity of the pretreated wastewater is 20000-30000 mg / L, and the COD is 10000-20000 mg / L. The expanding culture method comprises the steps of strain activation, seed solution preparation, 2 m < 3 > batch scale expanding culture, harvesting and storage, microporous aeration and mechanical stirring are adopted to guarantee uniform dissolved oxygen, and a trehalose-glycerol composite protective agent is added. According to the method, the cost of a ton of fungicide is greatly reduced by using waste culture bacteria, the cell density of the fungicide is greater than or equal to 30,000,000 / mL, the 36h COD removal rate is greater than or equal to 70%, the activity attenuation is less than or equal to 5% after 30 days of refrigeration at 4 DEG C, the problems of high cost, difficulty in large-scale production and poor stability in the prior art are solved, and the method is suitable for biological enhancement treatment of various types of high-salt pharmaceutical wastewater.

This invention enables accurate measurement of greenhouse gas emissions from animals. It also allows for easy evaluation of the reduction effect of greenhouse gas emissions from animals. Furthermore, it provides a methane emission inhibitor that suppresses methane production from Methanobrevibacter bacteria. [Solution] A bacterial quantification method for quantifying Methanobrevibacter species contained in the rectal feces of animals includes the steps of: collecting rectal feces from an animal; holding the rectal fecal sample in an anaerobic porter under anaerobic conditions; replacing the gas in the anaerobic porter with nitrogen gas, diluting the sample with a culture medium, and culturing the bacteria in a test tube; recovering the cultured bacteria; extracting DNA from the recovered bacteria; and quantifying the Methanobrevibacter species from the extracted DNA by quantitative PCR or a gene analysis method using next-generation sequencing analysis.

The invention relates to biotechnology, namely to a process for cultivating theBacillus velezensisbacterial strain, which can be used in the field of medicine for producing protein preparations, a source of bioactive peptides.The process for cultivating theBacillus velezensisCNMN-BB-12 bacterial strain, according to the invention, includes inoculating the bacterial suspension in a concentration of (2.0±0.2)·102CFU / mL in a medium containing, g / L: peptone 19.5, soluble starch 1.5 and distilled water the rest, or casein peptone 10.0, yeast extract 5.0, NaCl 10.0 and distilled water the rest, and cultivating on a rotary shaker at a speed of 200 rpm, for 48 hours, at a temperature of 30±1°C, with subsequent separation of the biomass from the culture liquid by centrifugation at 6000 rpm, for 20 min.The result of the invention consists in increasing the content of synthesized proteins in the culture liquid by 3.69-6.90 times.

The application relates to a preparation method of a bacterial cellulose-based multilayer structure functional bionic scaffold, which comprises the following steps: firstly, injecting a biomaterial solution into a silica gel model with a microchannel system to perform freeze drying to obtain a porous biological scaffold; secondly, performing crosslinking treatment on the porous biological scaffold; thirdly, inoculating a culture bacteria solution with a Xylobacter concentration of 10 9 ~ 10 15 individuals / mL on the crosslinking-treated porous biological scaffold, and performing static culture and freeze drying to obtain a bacterial cellulose-based composite scaffold; and finally, adopting a coating solution to coat the bacterial cellulose-based composite scaffold through a microchannel system, and performing demolding treatment to obtain the bacterial cellulose-based multilayer structure functional bionic scaffold. The application provides a functional coating process for the multilayer bionic scaffold by using the shrinkage space of the treated material, the micro-pore channel and the injection method of the patterned drainage network, effectively prevents the penetration of the coating solution, and realizes the perfect solidification of the coating solution on the scaffold.

The feed production device includes a first water tank in which bacteria and plankton are raised, and a second water tank in which the bacteria and plankton that are raised in the first water tank are annihilated to create a feed for leptocephalus.

This invention relates to the field of gene technology, specifically disclosing a method for enhancing the secretion of secondary metabolites based on the regulation of quorum sensing signal molecules. This method activates the expression of bacterial secondary metabolite synthesis gene clusters through bacterial-fungal interaction, comprising the following steps: (a) co-culturing bacteria and fungi in contact or non-contact mode; (b) during co-culturing, the fungi secrete the quorum sensing signal molecule farnesol; (c) the bacteria sense farnesol through a two-component system, activating the expression of secondary metabolite synthesis genes. This invention effectively overcomes the gene silencing problem caused by metabolic load or lack of regulation in natural strains by activating the originally trace expression of secondary metabolite synthesis gene clusters in bacteria using the fungal-derived quorum sensing signal molecule farnesol.

To provide a method for treating implantable device infections. [Solution] The present invention relates to the field of phage therapy, and more particularly to providing phage-based compositions and methods for treating or preventing infections associated with implantable devices. The compositions may be administered directly to the infection site of the device, as a single dose and / or by other means as needed, potentially avoiding the need to replace the implantable device. The method may include (a) obtaining a biological sample from the subject; (b) culturing bacteria present in the biological sample; and (c) inoculating the cultured bacteria with a phage to lyse them.

The invention provides a rapid degradation process for a degradable express green plastic package. The rapid degradation process comprises the following steps: 1, classifying raw materials, and then crushing and weighing the raw materials; 2, preparing culture soil: mixing vermiculite, base soil, water and nutrient substances to prepare the culture soil; 3, preparation of culture bacteria: proportioning aspergillus niger, Penicillium Polish and Bacillus thuringiensis according to a certain ratio, and adjusting the concentration; 4, uniformly mixing: feeding the crushed plastic, the culture soil and the culture bacteria according to the addition amount of the volume of the reaction container, and uniformly mixing; 5, the mixture is put into a reaction container, air is supplemented while stirring, the air is CO2-removed gas, the temperature in the reaction container is controlled to be 15-55 DEG C, the water content of a reaction mixture is controlled to be 40-60%, and the pH value is controlled to be 7.0-9.0. The method has the beneficial effects that the degradable plastic package is degraded by adopting the microbial flora and the culture soil, and the method has the advantages of low cost, simplicity in operation, low energy consumption, high degradation efficiency and the like. And the reaction conditions are mild, so that the process is more environment-friendly and has industrial feasibility.

1. The name of the design product: fish tank bacteria breeding tank. 2. The use of the design product: the design product is used for fish tank filtration and purification. 3. The design points of the design product: in shape. 4. The picture or photo that best indicates the design points: perspective drawing.

The invention discloses a tailings soil-forming biological enhancement complex microbial inoculant and a preparation method thereof, and belongs to the technical field of microbial inoculants. The invention relates to a tailing soil forming biological enhancement complex microbial inoculant. The active ingredients of the tailing soil forming biological enhancement complex microbial inoculant comprise bacterial suspensions of the following microorganisms: Arthrobacter sp., Bacteria strain, Paenarthrobacter nicotinovorans strain, Pedobacter roseus strain, non-cultured bacterial clones, and Rhizobacter arrhus, and the active ingredients of the tailing soil forming biological enhancement complex microbial inoculant comprise bacterial suspensions of the following microorganisms: Arthrobacter sp., Bacteria strain, Paenarthrobacter nicotinovorans strain, Pedobacter roseus strain, and the active ingredients of the tailing soil forming biological enhancement complex microbial inoculant, and the active ingredients of the tailing soil forming biological enhancement complex microbial inoculant. According to the volume of the bacterial suspension of each component, the volume fraction of each component is 1-2, the concentration of a single strain is not lower than 3 * 10 < 9 > CFU / ml, and agriculturally acceptable auxiliary materials can also be added. The preparation method comprises the following steps: selecting a non-antagonistic target strain for expanding culture, after expanding culture is finished, taking suspension of each strain, and uniformly mixing according to a ratio to obtain the complex microbial inoculant. Embedding treatment can be further carried out on the complex microbial inoculants. The complex microbial inoculant disclosed by the invention can effectively activate available nutrients of soil, promote crop growth and reduce the content of available heavy metals in the soil, and has a wide application prospect; the preparation method is simple in process, mild in condition and high in controllability, and efficient preparation and stable storage of the complex microbial inoculant can be achieved.

The present application relates to the technical field of mineral processing, and particularly relates to a desulfurization smelting process for high-sulfur tin concentrate, comprising the following steps: ore pretreatment, tin ore is crushed, ground and screened to obtain ore powder; bacteria cultivation and inoculation, bacteria suitable for high-sulfur environment are selected and inoculated into a small-scale cultivation tank and cultivated in a sterile environment; leaching reaction, the pretreated ore powder is mixed with water, bacterial culture solution is added to prepare ore slurry, and the ore slurry is leached; solution treatment and tin recovery, tin metal is recovered after solid-liquid separation of the ore slurry, and waste liquid is treated.The present application provides a desulfurization smelting process for high-sulfur tin concentrate, effectively solves the problems of environmental pollution and high cost existing in the traditional smelting process through the bacterial leaching method, bacteria can grow and reproduce quickly in a high-sulfur environment, so that the desulfurization efficiency is improved, the process operation is simple, low in cost and friendly to the environment, and the tin recovery rate can be significantly improved.

The application discloses a kind of extracellular vesicles of Roseomonos mucosa origin and its hydrogel composition, the extracellular vesicles are obtained by ultrafiltration concentration and gradient ultracentrifugation to culture bacteria liquid.For compared with original culture liquid or its live bacteria preparation, the treatment efficiency, safety of extracellular vesicle of the application are significantly improved.Based on the vesicle, the application also provides a kind of hydrogel composition loaded with the extracellular vesicle, which is constructed by extracellular vesicle, type I collagen, carboxymethyl chitosan and four-arm polyethylene glycol benzaldehyde by chemical crosslinking method, with good adhesion, self-repairing ability and injectability.The extracellular vesicle, hydrogel composition and hydrogel preparation thereof of the application can be applied to atopic dermatitis as drug or drug carrier, can specifically target type 2 inflammatory factors, alleviate the pathological changes such as skin lesion phenotype, epidermal thickening, mast cell infiltration and inflammatory factor expression increase of MC903 induced atopic dermatitis mouse model.

The invention relates to a ThrA mutant for improving serine tolerance and application of the ThrA mutant in preparation of L-serine. In one aspect, the present invention provides a homoserine dehydrogenase ThrA mutant having any one of amino acid substitutions of Y259T, S298R, A373C, or any combination thereof, relative to a wild-type homoserine dehydrogenase ThrA. In another aspect, the present invention provides a method of producing L-serine or L-serine derivatives comprising culturing a bacterium in a culture medium wherein the bacterium contains a coding sequence of the homoserine dehydrogenase ThrA mutant. According to the ThrA mutant provided by the invention, the tolerance of cells to the L-serine and the yield of the L-serine are improved.

This invention belongs to the field of microbial culture technology. It provides a method for culturing cariogenic acid-producing anaerobic bacteria and its application. The culture medium comprises the following components in the following proportions: peptone 0.5-5%, sucrose 1-10%, sodium chloride 0.1-0.6%, bromocresol green 0.1-0.3%, bromocresol purple 0.01-0.15%, vitamin C 0.01-0.05%, Tween-80 0.01-0.06%, phenylethanol 0.01-0.25%, and deionized water, forming a liquid culture medium. Based on the specific requirements of bacterial culture, this invention is used for culturing bacteria from teeth and the oral cavity. Peptone is used as the nitrogen source, and sucrose as the carbon source. When bacteria multiply rapidly, they convert sucrose into an acidic substance, changing the pH of the culture medium. In the presence of bromocresol green / bromocresol purple, the color of the culture medium changes, allowing for the determination of bacterial content in the sample and the prediction of the risk of dental caries.

The utility model relates to photosynthetic bacteria group culture equipment technical field discloses photosynthetic bacteria group expansion culture device, including culture jar, the outer wall bottom of culture jar is fixedly connected with expansion mechanism, the expansion mechanism is used for expanding culture bacteria group, the inner wall middle part of expansion mechanism is rotatably connected with feeding mechanism, the feeding mechanism is used for stirring bacteria group, the outer wall top of culture jar is fixedly connected with control mechanism, the expansion mechanism includes expansion jar, the expansion jar fixedly connected in the outer wall bottom of culture jar, the inner wall top of expansion jar is fixedly connected with the connecting cover, the outer wall top of connecting cover is equipped with the feeding hole, in the utility model, the bacteria group growth in culture jar, need to expand the culture when entering expansion jar through the feeding hole of connecting cover, and connecting frame fixed support supplies light, and a large number of supply mode real -time response bacteria group metabolic change, improve photosynthetic bacteria group culture's stability and product yield.

A system for evaluating the plant growth-promoting rhizobacterial properties (PGPR) of endophytic bacteria isolated from Acanthus ilicifolius L., and for assessing plant growth parameters, consisting of: a) an isolation unit for isolating and purifying endophytic bacteria from leaves of Acanthus ilicifolius L., the isolation unit comprising: • Surface sterilization device configured to treat leaf explants successively with Tween20 solution, 70% ethanol and 1% mercuric chloride, • Grinding device for homogenizing leaf tissue with phosphate buffer solution, • Incubation chamber configured for the cultivation of bacterial colonies on nutrient agar at 37°C; b) a characterization unit for the morphological, biochemical and molecular characterization of isolated bacteria, wherein the characterization unit comprises the following: • Equipment for Gram staining, • Equipment for biochemical tests for performing the indole test, the methyl red test, the citrate utilization test, the H2S production test, the gelatin hydrolysis test, the starch hydrolysis test and the catalase test, and • Molecular analysis equipment configured for 16S rRNA sequencing, including DNA isolation apparatus, PCR thermocycler and DNA sequencer; c) an optimization unit configured to analyze the culture conditions for maximum bacterial growth at different temperatures and pH values, the optimization unit comprising a colorimeter configured to measure absorbance at 610 nm to generate bacterial growth curves; d) a PGPR property evaluation unit for assessing the plant growth-promoting potential of isolated bacteria, wherein the PGPR property evaluation unit comprises the following: • Testing equipment for indole-3-acetic acid (IAA) production using the Salkowski color test, • Test apparatus for phosphate solubilization using the Pikovskaya medium • Devices for testing siderophore production using chromazurol S-agar (CAS agar) and • Test apparatus for the activity of 1-amino-1-cyclopropane-1-carboxylate (ACC) deaminase using Dworkin and Foster minimal salt medium; and e) a plant growth evaluation unit configured for in vitro analysis of the effects of bacterial inoculum on plant growth parameters such as stem length, root length, leaf length and chlorophyll content.

The invention relates to an AlaDH mutant and an application of the AlaDH mutant in production of L-serine. Specifically, the invention provides an alanine dehydrogenase AlaDH mutant, and the alanine dehydrogenase AlaDH mutant has amino acid substitutions of L107A, K161V and E205D or a combination of any two of the amino acid substitutions of L107A, K161V and E205D relative to wild alanine dehydrogenase AlaDH. The present invention also provides a method of producing L-serine or an L-serine derivative, the method comprising culturing a bacterium in a culture medium wherein the bacterium comprises a nucleotide sequence encoding the AlaDH mutant. According to the AlaDH mutant disclosed by the invention, 3-PHP can be specifically catalyzed to generate 3Per, so that the generation of byproducts is reduced, and the production cost is reduced.

This invention enables accurate measurement of greenhouse gas emissions from animals. It also allows for easy evaluation of the reduction effect of greenhouse gas emissions from animals. Furthermore, it provides a methane emission inhibitor that suppresses methane production from Methanobrevibacter bacteria. [Solution] A bacterial quantification method for quantifying Methanobrevibacter species contained in the rectal feces of animals includes the steps of: collecting rectal feces from an animal; holding the rectal fecal sample in an anaerobic porter under anaerobic conditions; replacing the gas in the anaerobic porter with nitrogen gas, diluting the sample with a culture medium, and culturing the bacteria in a test tube; recovering the cultured bacteria; extracting DNA from the recovered bacteria; and quantifying the Methanobrevibacter species from the extracted DNA by quantitative PCR or a gene analysis method using next-generation sequencing analysis.