63 results about "Adenylyl Cyclases" patented technology

The present invention is directed to novel methods of treating a subject with a bone deficit disorder. The methods generally include administering to a subject in need thereof a pharmaceutically acceptable formulation comprising a parathyroid hormone (PTH) peptide analogue in a daily dose of 2 μg to 60 μg, wherein said PTH peptide analogue has a reduced phospholipase-C activity and maintains adenylate cyclase activity.

This invention relates to novel analogs of pituitary adenylate cyclase-activating polypeptide (PACAP), which are agonists for the PACAP/vasoactive intestinal peptide (VIP) receptors: PAC1, VPAC1 and VPAC2 receptors. These PACAP analogs can be used as prophylactic/therapeutic agents for a wide range of medical disorders, including (but not limited to) cancer and autoimmune disease. These PACAP analogs can be coupled to suitable radionuclides and used in the localization, diagnosis and treatment of disseminated cancers and metastatic tumors, or coupled to small molecule therapeutics and used as vectors for targeted drug delivery. This invention also provides pharmaceutical compositions of one or more PACAP-like compounds of the invention either alone or in combination with one or more other prophylactic/therapeutic agents.

The invention provides adenylate cyclase, and a coding gene, a vector, a bacterial strain and application thereof. A DNA (Deoxyribose Nucleic Acid) sequence for coding the adenylate cyclase provided by the invention has a base sequence represented by SEQ ID NO.:1. Furthermore, the invention further provides the adenylate cyclase coded by the DAN sequence, a recombinant vector including the DAN sequence, a host cell including the recombinant vector, and applications of all in production of the adenylate cyclase. The genetic engineering strain of colibacillus, disclosed by the invention, can beused for expressing the adenylate cyclase with high efficiency. The inducible enzyme activation thereof can achieve 7 U/mg or 10 U/mg. The bacterial strain is used for producing cyclic adenosine monophosphate and has the advantages of simple process, moderate condition, short cycle, few by-products and the like.

The present invention relates to a method of preventing or treating a disease caused by bacterial infection by administering an effective amount of a modulator of bacterial adenylyl cyclase. The invention also provides pharmaceutical compositions useful for preventing or treating a disease, with the compositions containing a therapeutically effective amount of a modulator of bacterial adenylyl cyclase. The invention also provides screening methods for identifying selective modulators of bacterial adenylyl cyclase that do not substantially modulate adenylyl cyclase of the subject. The invention also provides methods for culturing bacterial pathogens and methods for inducing the pathogenic state in vitro.

The invention discloses a method for preparing cyclic adenosine monophosphate by adenylate cyclase. The method comprises the following steps that the adenylate cyclase serves as a catalyst, adenosinetriphosphate serves as a substrate, and in the presence of Mg <2+>, a reaction solution containing the cyclic adenosine monophosphate is obtained. According to the adenylate cyclase, an amino acid sequence as shown in SEQ ID NO.14 is provided, the stability is good, the half-life period is long at medium and low temperatures, ATP can be efficiently catalyzed to generate cAMP in one step at mediumtemperature, a substrate conversion rate reaches 90 % or above, the advantages of simple industry, mild conditions, short period, few by-products and the like are achieved, the clean and pollution-free effects are achieved, and the energy conservation, consumption reduction and emission reduction in the cAMP production process can be realized.

The invention provides cells and methods for identifying modulators of signal transduction, based on transducisome proteins that coordinate and assemble many types of signal transduction proteins. A transducisome is a PDZ domain containing protein that binds at least one signal transduction protein or a PDZ domain containing protein with at least one signal transduction protein bound. Examples of transducisome proteins include INAD, GRIP and other recently identified multi-PDZ domain proteins. Examples of signal transduction proteins include GPCRs, tyrosine kinase receptors, tyrosine phosphatase receptors, ion channels, phospholipases, adenylate cyclases, kinases and G-proteins. Also provided are methods for identifying modulators of signal transduction, proteins (and polynucleotides encoding the same) corresponding to transducisomes, modified transducisomes or defective transducisomes to use in assays of signal transduction, and a screening assay system for detecting protein-protein interactions.

The invention provides methods for treating, ameliorating or protecting (preventing) an individual or a patient having or at risk of having heart disease or heart failure, or decreased cardiac function, comprising: providing a cyclic adenosine monophosphate-incompetent (cAMP-incompetent) adenylyl cyclase type 6 (AC6) protein or polypeptide (also called "an AC6mut"), or an AC6mut -encoding nucleic acid or a gene operatively linked to a transcriptional regulatory sequence.

An immunogenic composition comprising a recombinant protein comprising a Bordetella CyaA, or a fragment thereof, and a peptide that corresponds to a tumor antigen is provided as a cancer treatment. Methods of treatment with this immunogenic composition are also provided. In an embodiment, the therapeutic composition is a treatment for melanoma, and comprises epitopes from the HLA*0201 epitope. These epitopes include Tyr or GnT-V, and are present in the recombinant proteins CyaA-E5-Tyr and CyaA-E5-GnT-V.

The invention discloses a qualitative and quantitative determination method for active proteins in a pertussis toxin product and a diphtheria-pertussis-tetanus vaccine. A high-performance liquid chromatography-tandem mass spectrometry method is adopted for establishing the high-flux high-selectivity high-sensitivity quantitative method for active proteins including pertussis toxin, filamentous hemagglutinin, adhesin, mycoprotein and adenylate cyclase toxin in the pertussis toxin product and the diphtheria-pertussis-tetanus vaccine. According to the method, characteristic peptide fragments which can be used for qualitative and quantitative analysis of various active vaccine proteins are screened out from a complex vaccine matrix for the first time, and the peptide fragments are different from other reported protein peptide fragments, cannot be obtained through a protein search library and cannot also be simply obtained through amino acid sequences of the vaccine proteins. Simultaneous quantification of five pertussis toxin subunits, filamentous hemagglutinin, adhesin, mycoprotein and adenylate cyclase toxin in pertussis toxin products and diphtheria-pertussis-tetanus vaccines from different manufacturers and of different batches can be realized.

The invention discloses a natural medicine composition for treating involutional depression and non-classical depression and use of the natural medicine composition, and belongs to the technical field of research of natural medicine formulas in compatibility dosage effect and use of the natural medicine. The composition consists of the components in percentage by mass: 70%-97.5% of hippophae rhamnoides fruit oil and 2.5%-30% of pseudo-ginseng stem and leaf total saponins. The natural medicine composition disclosed by the invention is reasonable in compatibility and safe in dosage, is free from any side effects on human bodies, can solve the problems that a conventional medicine has side effects and poor treatment effects when being used for treating climacterium and involutional depression, and has a significant regulation effect on serum marker indexes, namely adenylate cyclase-cyclic adenosine monophosphate and cyclic guanosine monophosphate, and reproductive hormonesm and target acceptors of various impact factors, such as follicular generation promoting hormones, pitocin, dihydrotestosterone hormones, female hormones, adrenocorticotropin hormones, cytokine interleukin-6, interleukin-8 cytokine and interleukin-2, so as to achieve a treatment effect on treatment of the involutional depression and the non-classical depression diseases.

The invention discloses a preparation method of purified coupling immobilized adenylate cyclase. The preparation method comprises the following steps: (1) preparing an affinity adsorption medium: adding 5-100g of a metal chelating resin carrier with surface modified by iminodiacetic acid to 10-200mL of 0.01-1mol/L of an NiCl2.6H2O solution, conducting oscillating at 25-30 DEG C and under 150-200rpm for 2-4h, and conducting filtration, so that the affinity adsorption medium is obtained; (2) immobilizing enzyme: adding 1.5-15ml of an adenylate cyclase solution which is 0.1-2mg/mL in concentration to 0.1-1g of the affinity adsorption medium obtained in the step (1), conducting oscillating at 25-30 DEG C and under 150-200rpm for 2-4h, and conducting centrifuging, so that the immobilized adenylate cyclase is obtained, wherein the adenylate cyclase is provided with a histidine tag. The immobilized enzyme prepared by the invention has the advantages of being high in enzymatic activity, low in enzymatic activity loss and the like, and the immobilized enzyme can be repeatedly used.

The invention discloses a method for improving adenylate cyclase expression activity and increasing cAMP content in plants. The method comprises the following steps: selecting a template DNA sequencecapable of encoding a catalytic activity center of adenylate cyclase by using biological information resources, adopting PCR amplification or chemical synthesis or other selectable cloning methods toobtain a DNA product with the sequence fragment, and after transcriptional expression of the DNA product, generating a peptide chain or protein with adenylate cyclase catalytic activity by translation; introducing the obtained DNA product into a selected plant gene expression vector system by using a DNA cloning technology, and inserting the plant gene expression vector system into multiple cloning sites between promoter and terminator element sequences for controlling exogenous gene expression; and carrying out genetic transformation on the constructed plant gene expression vector to obtain atransgenic plant with significantly improved adenylate cyclase expression activity and significantly increased cAMP content. The method provided by the invention provides a new idea for people to develop and utilize cAMP in plants, and fills the blank of the research.

The invention discloses adenylate cyclase containing an FYVE structural domain from phytophthora sojae as well as an encoding gene and application of the adenylate cyclase. The adenylate cyclase is prepared from phytophthora sojae. The adenylate cyclase PsZFAC containing the FYVE structural domain has an amino acid sequence as shown in SEQ ID No. 4, and a coding gene of the adenylate cyclase PsZFAC has a nucleotide sequence as shown in SEQ ID No. 3. The main function of the PsZFAC protein is to control the formation of phytophthora sojae sporangium. The gene provided by the invention has a very high application value in controlling phytophthora sojae root rot, and a bactericide developed by taking the protein as an action target has important practical significance in controlling the occurrence and prevalence of phytophthora sojae root rot.

The invention relates to a construction method of broad-spectrum antiviral recombinant salmonella. The construction method comprises the following steps: constructing a recombinant plasmid pS-DacA carrying adenylate cyclase, and introducing the plasmid into a salmonella choleraesuis cracking vector rSC0120 to form a recombinant salmonella choleraesuis cracking vector rSC0120 (pS-DacA). When the DacA is expressed in the salmonella choleraesuis cracking vector, the ATP is catalyzed to form c-di-AMP. After oral administration, the recombinant salmonella choleraesuis cracking vector rSC0120 (pS-DacA) enters host immune cells, c-di-AMP is released, and a host STING pathway is activated to achieve a broad-spectrum antiviral effect. The recombinant strain rSC0120 (pS-DacA) is proved to be capable of activating the STING pathway in macrophages and mice, causing the antiviral state of the macrophages and mice, and resisting the attack of PRRSV and PRV. The recombinant strain has broad-spectrum antiviral efficacy and is suitable for preventing and treating various animal viruses clinically.

The present invention is directed to novel methods of treating a subject with a bone deficit disorder. The methods generally include administering to a subject in need thereof a pharmaceutically acceptable formulation comprising a parathyroid hormone (PTH) peptide analogue in a daily dose sufficient to result in an effective pharmacokinetic profile and maintained adenylate cyclase activity, while simultaneously reducing undesirable side effects.

The invention discloses a gene coding sequence of adenylate cyclase (AC) in jujube fruits. A ZjAC4 gene for coding adenylate cyclase (AC) has a DNA (Deoxyribose Nucleic Acid) sequence as shown in SEQ ID No. 1. The gene coding sequence of the adenylate cyclase in the jujube fruits is adopted to efficiently synthesize cAMP, and the gene coding sequence has important application value.

A method of screening for a therapeutic and/or preventive drug for cartilaginous hyperplasia and a therapeutic and/or preventive drug for cartilaginous hyperplasia are provided.

The following are provided: a method of screening for a therapeutic and/or preventive drug for cartilaginous hyperplasia, comprising a step of culturing chondroprogenitor cells under conditions in which the cells are brought into contact with a test substance and conditions in which the cells are not brought into contact with the test substance and a step of determining the SOX9 promoter activity, cAMP level, or degree of phosphorylation of CREB in the cells or the extracellular matrix volume in a culture; and a therapeutic and/or preventive drug for cartilaginous hyperplasia, comprising as an active ingredient an adenylate cyclase inhibitor.