31 results about "Forskolin" patented technology

The present application belongs to the technical field of oral care, and particularly relates to an oral care composition and a preparation method thereof. The oral care composition comprises, by mass percentage, chitosan oligosaccharide 0.1-0.3%, sea hao polysaccharide 0.08-0.14%, forskolin 0.07-0.12%, corn sprout extract 0.05-0.09%, thickening agent 1-3%, humectant 40-50%, and the balance being water. The oral care composition of the present application can effectively inhibit the activity of glucosyltransferase, inhibit the production of dextran, interfere with the formation process of dental plaque, and reduce the adhesion sites of pathogenic bacteria in the oral cavity. In addition, the composition of the present application can promote the proliferation and repair of oral mucosa epithelial cells, and help repair oral mucosa damage. Furthermore, the use of each component in the composition of the present application can balance the oral microbial flora, while achieving the effect of inhibiting pathogenic bacteria and improving the oral environment.

The invention provides a special culture medium for prostate cancer organoid and a culture method thereof, and belongs to the technical field of biological medicines. The special culture medium for the prostatic cancer organoid is composed of a basic culture medium and a culture additive, and the basic culture medium does not contain exogenously added Noggin recombinant protein and R-spondin recombinant protein. The culture additive is prepared from a basic fibroblast growth factor, a keratinocyte growth factor, a fibroblast growth factor 10, insulin, a p38MAPK signal channel inhibitor, an insulin-like growth factor 1, B27, nicotinamide, N-acetylcysteine, hydrocortisone, a ROCK kinase inhibitor, retinoic acid, interleukin 6, prostaglandin E2, a eriocin and androgen. According to the special culture medium for the prostatic cancer organoid, the culture success rate is increased while the cost is reduced.

The invention provides an organoid culture medium, an organoid culture method and application of the organoid culture medium, the organoid culture medium comprises a first-stage culture medium, the first-stage culture medium comprises a first-stage basic culture medium and first-stage addition factors, and the first-stage addition factors comprise N-acetylcysteine, nicotinamide, a ROCK inhibitor, Noggin, R-spondin, EGF, HGF, Gastrin, FGF10, Blebbistatin, Forskolin, A83-01, LDN193189, DAPT, TGF-alpha and UDAC. By using the organoid culture medium, the liver organoid and the fatty liver organoid can be effectively obtained, and the obtained fatty liver organoid has obvious fat deposition, is sensitive to fatty liver treatment drugs, highly restores the physiological status when human body fatty liver diseases occur, and has high application value.

The invention discloses an establishment method and application of an iPSCs (induced pluripotent stem cells)-derived valvular interstitial cell calcification model, and belongs to the technical field of cell model establishment. The construction method of the iPSCs-derived valvular interstitial cell calcification model comprises the following steps: (1) carrying out induced differentiation for 7 days by using the combination of four regulatory factors, namely BMP4, CHIR99021, VEGF and Forskolin, so as to differentiate mature valvular interstitial cells; and (2) culturing the iPSCs-derived valvular interstitial cells in the step (1) in an osteogenic induction culture medium at 37 DEG C under the condition of 5% CO2 for at least 3 days. The invention provides a rapid and efficient differentiation scheme, the mature valvular interstitial cells induced by the scheme are very close to human primary valvular interstitial cells, and a high-quality experimental platform is provided for screening and mechanism research of anti-calcification drugs.

The invention discloses a preparation method and application of autonomic ganglion organoid, and relates to the technical field of biology. The method comprises the following steps: 1) providing neural crest stem cells; 2) differentiating the neural crest stem cells into autonomic ganglion-like organs through the combined action of added external factors and internal factors; wherein the external factors comprise a bFGF (basic fibroblast growth factor), an IGF-1 (insulin-like growth factor), a BDNF (brain-derived neurotrophic factor), a GDNF (brain-derived neurotrophic factor), an NGF (nerve growth factor), an NT-3, an EGF (epidermal growth factor), Forskolin, a DAPT (diaminopropyltriethoxysilane), an insulin, a Transferrin, an Activin A and a BMP4 (bone morphogenetic protein 4); the addition of the intrinsic factors is that the following four transcription factors, namely Ascl1, Hand2, Phox2a and Phox2b, are overexpressed in the neural crest stem cells at the same time. The organoid prepared by the scheme contains various neurons similar to the autonomic ganglion in vivo, Schwann glial cells and satellite glial cells. The prepared autonomic ganglion organoid can be applied to research on development regulation of autonomic ganglions and occurrence and development mechanisms of various human diseases, development of therapeutic drugs and the like.

The invention relates to a preparation method of a natural active molecule 6-acetyl forskolin. The preparation method comprises the following steps: by taking forskolin as a starting raw material, carrying out ester exchange on 7-acetyl and 6-OH to obtain isoforskolin, further selectively protecting 1-OH of isoforskolin, acetylating 7-OH, finally removing a protecting group of 1-OH, and carrying out reaction post-treatment and purification to obtain high-purity 6-acetyl forskolin. The synthesis method of the 6-acetyl forskolin comprises four steps of chemical reactions, the total yield is larger than 50%, the synthesis process is simple and stable, the steps are few, purification is easy and convenient, amplification production can be achieved, and an industrialized preparation method is provided for further development and utilization of the 6-acetyl forskolin.

The application discloses application of forskolin and / or isoforskolin in preparation of a medicine for preventing and / or treating colitis, and belongs to the technical field of medicines.The medicine can significantly improve the disease phenotype of colitis, including relieving weight loss, reducing disease activity index score, improving colon length shortening and histopathological damage.The mechanism of action involves multiple targets: repairing intestinal mucosal barrier function, reducing local inflammation of the colon, and regulating intestinal flora balance, restoring the abundance of beneficial bacteria and inhibiting the proliferation of pathogenic bacteria.The application provides a candidate drug with anti-inflammatory, barrier repair and flora regulation functions for the treatment of colitis, and expands the medical use of forskolin or isoforskolin.

Food supplement characterized by containing an active ingredient consisting of - 18 to 19 wt% green tea extract, of which 45 to 55 wt% of this extract consists of polyphenols and 6 to 7 wt% of this extract consists of theine, - 18 to 19% w / w extract from Cambodian Garcinia 10:1, - 14 to 15 wt% extract from red Moro orange 20:1, - 14 to 15 wt% extract from Gymnema sylvestre, of which 25 wt% of this extract consists of gymnemic acids, - 7 to 8 wt% extract of Coleus forskohlii, of which 5 wt% of this extract consists of forskolin, - 7 to 8% by weight berberine from Indian barberry, - 7 to 8% w / w extract from Paraguayan holly 10:1, - 7 to 8% w / w extract of fenugreek 10:1, - 3 to 4 wt% saffron extract, of which 0.1 to 0.2 wt% this extract consists of safranal, and max. 0.005 wt% chromium.

The invention belongs to the technical field of oral care, and particularly relates to an oral care composition and a preparation method thereof. The composition for oral care is prepared from the following components in percentage by mass: 0.1 to 0.3 percent of chitosan oligosaccharide, 0.08 to 0.14 percent of hail vegetable polysaccharide, 0.07 to 0.12 percent of forskolin, 0.05 to 0.09 percent of corn bud extract, 1 to 3 percent of thickening agent, 40 to 50 percent of humectant and the balance of water. The oral care composition can effectively inhibit the activity of glucosyltransferase, inhibit the generation of glucan, interfere the formation process of dental plaque, and reduce the attachment sites of pathogenic bacteria in the oral cavity. The oral mucosa epithelial cells can be promoted to proliferate and repair, and oral mucosa injury can be repaired. In addition, all the components in the composition are matched for use, so that oral microbial flora can be balanced, meanwhile, the effect of inhibiting pathogenic bacteria is achieved, and the oral environment is improved.

The present application relates to Foskolin induced differentiation treatment of hepatocellular carcinoma. Specifically, the present application relates to a method and use of Forskolin for inducing differentiation of human malignant hepatocellular carcinoma, thereby being applied to the treatment of malignant solid tumors. The research of the present application shows that after treating hepatocellular carcinoma cells with exogenous addition of Forskolin for a period of time, the hepatocellular carcinoma cells can be effectively induced to differentiate, thereby providing a new drug for tumor induced differentiation treatment. The purpose of the present application is to provide the use of Forskolin and its derivatives for anti-hepatoma drugs.

The application discloses a culture medium for constructing a liver cancer lung metastasis microtumor model and application thereof. The application relates to the technical field of biology, and provides a culture medium for constructing a liver cancer lung metastasis microtumor model, which is composed of three antibacterial and antifungal agents, HEPES, GlutaMax, human recombinant proteins EGF, HGF, IL-2, IL-15, R-spondin1, CXCL12, recombinant human lung surfactant proteins SP-A, SP-D, forskolin, A83-01, N-acetyl-L-cysteine, nicotinamide, N2 additives, B27 and a basic culture medium. The culture success rate of the liver cancer lung metastasis microtumor model is effectively improved.

The invention provides a method of promoting weight management in a mammal comprising administering to the mammal a composition comprising at least one weight loss agent and at least one mitochondrial enhancer. The weight loss agent can be selected from the group consisting of psyllium, guar gum, capsaicin, chitosan, caffeine, gamboge, mume, capsaicin, yohimbine, butterfly pea, glucomannan, African mango, guarana, pyruvate, carnitine, beta-glucan, fucoxanthin, raspberry ketone, white kidney bean, kola nut, chromium, ginseng, psyllium, St. John's wort, dandelion, hydroxycitric acid, conjugated linoleic acid, green tea, black tea, coffee bean extract, forskolin, and bitter orange. The mitochondrial enhancer can be selected from the group consisting of beetroot extract, nitrate, idebenone, nicotinamide riboside, elamipretide, vitamin C, vitamin D, vitamin E, thiamine, riboflavin, magnesium, calcium, phosphate, phospholipid, creatine, pyruvate, coenzyme Q10, NADH, niacin, L-carnitine, dichloroacetate, curcumin, schisandrin, and resveratrol. Compositions for weight management are also provided.

Herein is reported a method for the production of one or more hamster-derived induced pluripotent stem cells comprising the steps of transducing a hamster cell with a Sendai virus comprising coding sequences for reprogramming factors, cultivating the transduced cell in the presence of at least a WNT pathway activator for about 12 days, replating the cells, adding a medium comprising one or more proteinogenic growth factors and cultivating the replated cells in the presence of the at least one WNT pathway activator, and selecting one or more cells that are positive for alkaline phosphatase and SSEA1 and that has stem cell morphology, wherein the at least one WNT pathway activator is in a small molecule cocktail comprising CHIR99021 (CHIR), 3-deazaneplanocin A (DZNep), forskolin, RepSox, tranylcypromine, aprotinoid acid (TTNPB), and valproic acid (VPA).

The invention provides a construction method and an application of recombinant yarrowia lipolytica for synthesizing furithrolin. The recombinant yarrowia lipolytica is obtained by introducing a combination of a coding gene of tenosyl ether synthase, a coding gene of cytochrome P450 oxidase, a coding gene of cytochrome P450 reductase, a coding gene of acetyltransferase, a coding gene of cytochrome b5 and a coding gene of scaffold protein, and knocking out a coding gene of phosphatidic acid phosphatase at the same time. The efficient synthesis of the fuchsin is realized. The construction method of the yarrowia lipolytica for biosynthesis of the eriolarynx element, provided by the invention, is simple to operate, and the constructed recombinant yarrowia lipolytica can efficiently produce the eriolarynx element and has relatively high production and application values.

The invention belongs to the field of biotechnology and biomedicine, and particularly relates to a mesenchymal stem cell exosome as well as an induction medium, a preparation method, a product and application thereof. The induction culture medium is prepared from didemethoxycurcumin, resveratrol and forskolin. The preparation method comprises the following steps: carrying out induced culture on adipose-derived mesenchymal stem cells by using the culture medium, collecting supernatant twice, and carrying out centrifugation, filtration and sucrose density gradient centrifugation purification to obtain the exosome. The mesenchymal stem cell exosome prepared by the method can be used as a core component to prepare medicines, can play an anti-fibrosis role in ways of inhibiting collagen synthesis, promoting repair of damaged alveolar epithelium, improving lung functions and the like, and has important application value in preparation of medicines for treating pulmonary fibrosis. A safe and effective novel strategy is provided for pulmonary fibrosis treatment, and clinical transformation of exosome therapy is promoted.

The invention provides a composition for reversing organ fibrosis and application of the composition, and belongs to the technical field of biological medicines.The cocktail composition is prepared from valproic acid, CHIR99021, Repsox, trans-benzene ring propylamine and forskolin. The HAT inhibition composition is prepared from A485 and WM-8014; the cocktail composition and the HAT inhibition composition are used independently or jointly, and hepatic fibrosis or pulmonary fibrosis can be reversed; when the composition is used in combination, a synergistic enhancement effect is shown in pulmonary fibrosis and hepatic fibrosis models, and the effects of improving fibrosis indexes and reversing epigenetic age are more remarkable; according to the composition provided by the invention, the epigenetic procedure of cells is accurately controlled, so that the cells are safely reversed from a pathological state to a healthy or resting state, the pathological phenotype of fibrosis is improved, the biological age of the cells is fundamentally reversed, and a major breakthrough in the field of fibrosis treatment is represented.

The present application relates to the technical field of stem cells, and particularly relates to a serum-free induction medium with clear components for promoting differentiation of induced mesenchymal stem cells into dermal papilla cells and an induction method. A small molecule compound composition comprises all-trans retinoic acid, forskolin, CHIR-99021 and minoxidil in a molar ratio of 0.2-5:2-50:0.2-10:0.2-10. The present application adopts multiple small molecule compounds to promote differentiation and function improvement of dermal papilla cells and transformation of mesenchymal stem cells into dermal papilla cells. The first three promote differentiation of dermal papilla cells, and the last one promotes proliferation and inhibits apoptosis of dermal papilla cells. The present application can solve the technical problems of high process cost and unsatisfactory clinical safety caused by the need to add cytokines in the process of inducing differentiation of stem cells into dermal papilla cells, and speeds up the induction process, and has an ideal application prospect.

The invention discloses a composition for constructing an IOPN organ model, a model construction method and application. The composition for constructing the IOPN organoid model comprises an organoid model culture medium, and the organoid model culture medium comprises a penicillin-streptomycin double antibody solution, GlutaMAX, a serum-free supplement, Rspo-1, a Wnt activator, a growth factor, an HEPES buffer solution, N-acetyl-L-cysteine, a gastrin hormone, nicotinamide, forskolin, an ALK inhibitor, a ROCK inhibitor and the balance of a basic culture medium. The IOPN type organ model cultured by the composition for constructing the IOPN type organ model can maintain the original characteristics of tumors, provides a reliable biological model for pathological research and drug research and development of pancreatic tumors, and is convenient to operate and low in cost.

The application discloses a pharmaceutical-grade PDRN and a preparation method thereof, and belongs to the technical field of biotechnology. The method comprises the following steps: (1) grinding salmon testis tissue in a pre-cooled grinding buffer to obtain a homogenate; (2) using Forskolin to induce the grinding homogenate; the induction conditions are as follows: first, adding Forskolin to 8-12 muM, and standing for 1-3 hours at 4 DEG C; then, increasing the temperature to 10-20 DEG C, adding Forskolin to 18-22 muM and 2 mM glutathione, and treating for 2 hours; and (3) extracting the PDRN through SDS-sodium chloride.

This application provides isolated antibodies, and antigen-binding fragments thereof that specifically bind chemokine receptor 4 (CXCR4). These CXCR4 antibodies, or antigen-binding fragments thereof, have a high affinity for CXCR4, function to effectively block SDF-1 binding to CXCR4, thereby inhibiting forskolin stimulated cAMP with low nM IC50, are less immunogenic compared to their unmodified parent antibodies in a given species (e.g., in a human), and can be used to treat CXCR4-associated disorders while avoiding the adverse side effects associated with the current CXCR4 antagonist therapies.

The invention provides a culture medium for inducing secretion of stem cell factors and application of the culture medium, and belongs to the technical field of cell biology. The culture medium adopts a three-stage dynamic induction strategy and comprises a starting culture medium (containing CHIR99021 and valproic acid), an induction culture medium (containing Forskolin, SR3677 and melatonin) and a maintenance amplification culture medium (containing ISX-9 and resveratrol). Through staged and accurate small molecule stimulation, the paracrine function of the cord blood mesenchymal stem cells can be efficiently and synergistically enhanced, and the secretion level of multiple therapeutic factors such as SCF, VEGF, HGF and IGF-1 is remarkably improved. Therefore, the invention provides a safe and stable culture system with definite components, the culture system is used for obtaining a high-activity stem cell secretion product, and the product can be applied to preparation of an external gel preparation for promoting skin wound healing and has important clinical transformation value.

The application belongs to the technical field of pharmaceutical chemistry and pharmacology, and relates to application of a forskolin tetracyclic analogue in preparation of an anti-inflammatory drug, the forskolin tetracyclic analogue has a chemical structural formula shown in formula (I), 7-deacetyl forskolin 1 is obtained by reaction of forskolin and sodium methoxide in methanol, then the forskolin rearrangement product 2 is obtained by reaction of the 7-deacetyl forskolin 1 and sodium periodate in a DCM-H2O mixed solution, the forskolin rearrangement product 2 and DMP, pyridine are reacted in acetonitrile to obtain the forskolin rearrangement product 3, and finally the forskolin rearrangement product 3 is reacted with aryl iodide in anhydrous dichloroethane under catalysis of anhydrous silver acetate, anhydrous copper acetate and palladium acetate to obtain the forskolin tetracyclic analogue. The forskolin tetracyclic analogue obtained in the application has strong anti-inflammatory activity and can be used for preparation of an anti-inflammatory drug.

The invention provides a CDM culture medium for inducing differentiation of amplified bile duct tree stem cells to form functional bile duct organoid, and the CDM culture medium is composed of a KM basic culture medium and the following components: growth factors: FGF2, VEGF165 and HGF; the small molecule compounds comprise Retinoid Acid and Forskolin, and the small molecule compounds comprise Retinoid Acid and Forskolin; inorganic ions: Ca < 2 + > and Cu < 2 + >; the protein of the protein is TGF (Transforming Growth Factor)-beta 1 Protein and Jagged-1 Protein. The CDM culture medium provided by the invention can induce and differentiate the amplified bile duct tree stem cell organoid into a functional bile duct organoid, provides a core technical support for the fields of cell therapy and tissue engineering, and promotes the development of bile duct disease model construction, drug screening and the like. Besides, the functional bile duct organ prepared by the CDM culture medium and the matched culture method can be directly used for bile duct injury repair, and a new treatment means is provided for end-stage bile duct diseases; and the model can also be used as an in-vitro drug screening model for simulating the physiological and pathological state of the in-vivo bile duct, the research and development process of bile duct disease treatment drugs is assisted, and the preclinical research cost is reduced.

The application provides a prostate cancer organoid special culture medium and a culture method thereof, and belongs to the technical field of biological medicine. The prostate cancer organoid special culture medium is composed of a basic culture medium and a culture additive. The basic culture medium does not contain exogenous added Noggin recombinant protein and R-spondin recombinant protein. The culture additive comprises basic fibroblast growth factor, keratinocyte growth factor, fibroblast growth factor 10, insulin, p38MAPK signal pathway inhibitor, insulin-like growth factor 1, B27, nicotinamide, N-acetylcysteine, hydrocortisone, ROCK kinase inhibitor, retinoic acid, interleukin 6, prostaglandin E2, forskolin and androgen. The prostate cancer organoid special culture medium can reduce the cost and improve the culture success rate.

The invention relates to the field of biology, and provides a culture medium for cervical stomach type adenocarcinoma organs and application of the culture medium, the culture medium comprises a basic culture medium and an additive, and the additive comprises Neuregulin 1 and at least one of the following components: B27, Y-27632, estradiol, A83-01, EGF, Noggin, R-Spondin1, hydrocortisone, Forskolin and wnt3a. According to the present invention, with the organoid culture medium, the cervical stomach type adenocarcinoma organoid can be effectively obtained, and the cervical stomach type adenocarcinoma organoid can maintain the histomorphological characteristics of the original tumor tissue, can rapidly amplify and grow, and can be stably subjected to passage cryopreservation and resuscitation, such that the important research platform is provided for the personalized drug screening and treatment, and the application prospect is broad. The method has profound significance for scientific research and clinical application.

The invention discloses a chemical small molecule composition for inducing directional differentiation of umbilical cord mesenchymal stem cells into dopaminergic neurons, a culture medium, an induction method and application. The chemical small molecule composition is prepared from SB431542, LDN193189, CHIR99021, Forskolin, Y27632, FGF8 (Fibroblast Growth Factor 8), SHH (Secret Hormone Hormone) and DAPT (Diaminopropyltriethoxysilane). A chemical reprogramming method without gene integration is established through combination of small molecule compounds and morphogenetic factors, human umbilical cord mesenchymal stem cells are efficiently induced and differentiated into dopaminergic neurons, and a safe, stable and clinically convertible functional cell source is provided for cell replacement treatment of Parkinson's disease. According to the scheme, the problems of tumorigenicity, ethical limitation, immunological rejection and the like in a traditional induction method are solved, and a new technical path and a new cell preparation strategy are provided for stem cell treatment of neurodegenerative diseases.

The application belongs to the technical field of pharmaceutical chemistry and pharmacology, and relates to application of a forskolin oxygen bridge ring derivative in preparation of anti-inflammatory drugs. The forskolin oxygen bridge ring derivative has a chemical structural formula shown in formula (I), is obtained by reacting forskolin and potassium hydroxide in methanol to obtain forskolin rearrangement product 1, and then reacting the forskolin rearrangement product 1 with aryl iodide in anhydrous dichloroethane under the catalysis of anhydrous silver acetate, anhydrous copper acetate and palladium acetate to obtain the forskolin oxygen bridge ring derivative (I). The forskolin oxygen bridge ring derivative obtained by the application has relatively strong anti-inflammatory activity, and the activity is equivalent to that of dexamethasone, and can be used for preparing anti-inflammatory drugs.

The invention belongs to the field of agricultural biology, and particularly relates to an aquaporin phosphokinase activator and a preparation method and application thereof.According to the activator, adenosine polyethylene glycol forskolin and a lipoic acid hexamethylenediamine protein kinase C activator serve as core components, and plant hormones such as gibberellin, abscisic acid and jasmonic acid methyl ester are cooperated to prepare the aquaporin phosphokinase activator. Auxiliary materials such as chitosan, glycine betaine, trehalose, glycerol, polysorbate, urea and calcium chloride are compounded, and specific low-temperature dissolution, step-by-step mixing and ultrasonic emulsification processes are carried out, so that the water-retaining agent is prepared. The preparation can specifically activate crop fruit aquaporin phosphorylation kinase, and promote crop aquaporin to keep open conformation by regulating and controlling key phosphorylation sites, so that the fruit moisture transmembrane transport efficiency is improved. After being diluted, the fruit preservative is sprayed on leaf surfaces in the middle and later periods of fruit ripening, so that the water conductivity of fruits can be effectively improved, the absorption of water and nutrient substances of the fruits is promoted, the water replenishing and weight increasing of the fruits are realized, the integrity of cell structures is enhanced, the fruit cracking rate is obviously reduced, and the flavor and quality of the fruits are improved or maintained.

Methods for producing nanoparticles of hydrophobic compounds, such as EGCG-palmitate, Cannabidiol (CBD), Delta-9-tetrahydrocannabinol (THC-9), quercetin, ivermectin, retinoic acid, curcumin, resveratrol, lycopene, lutein, CoQ10, procyanidin B2, oxyresveratrol, tetrahydropiperine, rapamycin, forskolin, and tetrahydrocurcumin, are described. In some forms, one or more water soluble compounds are used with at least one hydrophobic compound to form the nanoparticles. Nanoparticles produced using the disclosed methods have tightly packed structures assembled by the hydrophobic compounds themselves, without the need for other components, such as surfactant, polymer, encapsulation, or metals. These nanoparticles can significantly increase the water solubility of hydrophobic compounds, allowing increased solubility and bioavailability in their own nanoparticle form.

The invention discloses a forskolin derivative as well as a preparation method and application thereof, and relates to the technical field of biological medicine, the molecular formula of the forskolin derivative is C32H39N3O9, the molecular weight is 609.67600, and the specific structural formula is shown in the specification. The forskolin derivative prepared by the invention has excellent druggability and pharmacological activity, has a better neuroprotective effect, shows a stronger neuroprotective effect than donepezil and forskolin in an A beta25-35 induced PC12 cell injury model, and has lower toxicity to PC12 cells, so that the forskolin derivative can be used for preparing a neuroprotective drug. The compound can be applied to preparation of Alzheimer's disease medicines in the later period, and is of great significance to prevention and treatment of Alzheimer's disease.