45 results about "Forskolin" patented technology

The invention discloses a method for inducing dorsal root node satellite glial cells to be reprogrammed into neurons by using a small molecule compound and application of the method. The small molecule compound composition is prepared from ISX-9, RO4929097, CHIR99021, VPA, RepSox, Forskolin, SC79 and VC, and growth factors BDNF, GDNF, NT-3 and NGF beta, neuron culture additives B27 and N2 additives are added at the same time, so that survival and maturation of neurons are promoted and induced. Wherein each component is a safe and low-cost small molecule compound and a neurotrophic factor, and the pharmaceutical composition and the growth factor can be used for reprogramming rat dorsal root ganglion satellite glial cells into neurons with specific phenotypes and functional characteristics in vitro. And a new seed cell is provided for basic research of clinical nerve injury diseases and clinical cell treatment development.

The application provides a culture medium and a culture method of ovarian cancer primary cells. The culture medium comprises an MST1 / 2 kinase inhibitor, sodium pyruvate, forskolin, epidermal growth factor, gastrin, fibroblast growth factor 7, nicotinamide, SB431542, and fetal bovine serum. Compared with the existing culture method, the in-vitro culture using the culture medium of the application has higher amplification efficiency; the culture of the ovarian cancer primary cells using the culture medium can maintain the morphological structure and pathological characteristics of the primary tissue, and improve the success rate and survival rate of the ovarian cancer primary cell culture.

The present application belongs to the technical field of oral care, and particularly relates to an oral care composition and a preparation method thereof. The oral care composition comprises, by mass percentage, chitosan oligosaccharide 0.1-0.3%, sea hao polysaccharide 0.08-0.14%, forskolin 0.07-0.12%, corn sprout extract 0.05-0.09%, thickening agent 1-3%, humectant 40-50%, and the balance being water. The oral care composition of the present application can effectively inhibit the activity of glucosyltransferase, inhibit the production of dextran, interfere with the formation process of dental plaque, and reduce the adhesion sites of pathogenic bacteria in the oral cavity. In addition, the composition of the present application can promote the proliferation and repair of oral mucosa epithelial cells, and help repair oral mucosa damage. Furthermore, the use of each component in the composition of the present application can balance the oral microbial flora, while achieving the effect of inhibiting pathogenic bacteria and improving the oral environment.

The invention provides a special culture medium for prostate cancer organoid and a culture method thereof, and belongs to the technical field of biological medicines. The special culture medium for the prostatic cancer organoid is composed of a basic culture medium and a culture additive, and the basic culture medium does not contain exogenously added Noggin recombinant protein and R-spondin recombinant protein. The culture additive is prepared from a basic fibroblast growth factor, a keratinocyte growth factor, a fibroblast growth factor 10, insulin, a p38MAPK signal channel inhibitor, an insulin-like growth factor 1, B27, nicotinamide, N-acetylcysteine, hydrocortisone, a ROCK kinase inhibitor, retinoic acid, interleukin 6, prostaglandin E2, a eriocin and androgen. According to the special culture medium for the prostatic cancer organoid, the culture success rate is increased while the cost is reduced.

The invention provides an organoid culture medium, an organoid culture method and application of the organoid culture medium, the organoid culture medium comprises a first-stage culture medium, the first-stage culture medium comprises a first-stage basic culture medium and first-stage addition factors, and the first-stage addition factors comprise N-acetylcysteine, nicotinamide, a ROCK inhibitor, Noggin, R-spondin, EGF, HGF, Gastrin, FGF10, Blebbistatin, Forskolin, A83-01, LDN193189, DAPT, TGF-alpha and UDAC. By using the organoid culture medium, the liver organoid and the fatty liver organoid can be effectively obtained, and the obtained fatty liver organoid has obvious fat deposition, is sensitive to fatty liver treatment drugs, highly restores the physiological status when human body fatty liver diseases occur, and has high application value.

The invention provides a method for detecting forskolin, Y-27632 and AM2394 in cell culture biomass and a culture solution, and relates to the technical field of food detection. The detection method comprises the following steps: detecting target objects in cell culture biomass and a culture solution by using an ultra-high performance liquid chromatography-tandem mass spectrometry, and controlling a mobile phase A to be ultrapure water, formic acid and ammonium acetate in a detection process; the mobile phase B is methanol or acetonitrile; the detection method can accurately detect forskolin, Y-27632 and AM2394 in the cell culture biomass and the culture solution and perform quantitative analysis, and meanwhile, the detection method is simple and rapid to operate, high in sensitivity and good in repeatability.

The invention discloses a method for inducing iPSCs (induced pluripotent stem cells) to directionally differentiate into vascular endothelial cells by using a small molecule compound and cell factor composition, which is characterized in that iPSCs cells are used as starting cells, and a vascular endothelial cell differentiation scheme completely dependent on chemical small molecule and cell factor stimulation is established. An E6 culture medium containing CHIR-99021, BMP4 and Activin A is utilized to induce mesoderm progenitor cells, an E6 culture medium containing VEGFA (vascular endothelial growth factor A), FGF-2, 8-Bromoadenosine 3 ', 5'-cyclic monophosphate monophosphate, SB431542, Forskolin and Melatonin is utilized to induce the endothelial cells, and an E6 culture medium containing VEGFA, FGF-2 and Hydrocortisone is utilized to further induce and differentiate the endothelial cells into the vascular endothelial cells. By using the scheme, the differentiation efficiency can exceed 99%, and differentiated cells have phenotypes and functions of vascular endothelial cells and do not express pluripotent related markers SSEA-4, TRA-1-60 and TRA-1-81.

The invention discloses an establishment method and application of an iPSCs (induced pluripotent stem cells)-derived valvular interstitial cell calcification model, and belongs to the technical field of cell model establishment. The construction method of the iPSCs-derived valvular interstitial cell calcification model comprises the following steps: (1) carrying out induced differentiation for 7 days by using the combination of four regulatory factors, namely BMP4, CHIR99021, VEGF and Forskolin, so as to differentiate mature valvular interstitial cells; and (2) culturing the iPSCs-derived valvular interstitial cells in the step (1) in an osteogenic induction culture medium at 37 DEG C under the condition of 5% CO2 for at least 3 days. The invention provides a rapid and efficient differentiation scheme, the mature valvular interstitial cells induced by the scheme are very close to human primary valvular interstitial cells, and a high-quality experimental platform is provided for screening and mechanism research of anti-calcification drugs.

The invention discloses a preparation method and application of autonomic ganglion organoid, and relates to the technical field of biology. The method comprises the following steps: 1) providing neural crest stem cells; 2) differentiating the neural crest stem cells into autonomic ganglion-like organs through the combined action of added external factors and internal factors; wherein the external factors comprise a bFGF (basic fibroblast growth factor), an IGF-1 (insulin-like growth factor), a BDNF (brain-derived neurotrophic factor), a GDNF (brain-derived neurotrophic factor), an NGF (nerve growth factor), an NT-3, an EGF (epidermal growth factor), Forskolin, a DAPT (diaminopropyltriethoxysilane), an insulin, a Transferrin, an Activin A and a BMP4 (bone morphogenetic protein 4); the addition of the intrinsic factors is that the following four transcription factors, namely Ascl1, Hand2, Phox2a and Phox2b, are overexpressed in the neural crest stem cells at the same time. The organoid prepared by the scheme contains various neurons similar to the autonomic ganglion in vivo, Schwann glial cells and satellite glial cells. The prepared autonomic ganglion organoid can be applied to research on development regulation of autonomic ganglions and occurrence and development mechanisms of various human diseases, development of therapeutic drugs and the like.

The application provides a culture medium and a culture method of a gastric cancer organoid. The culture medium comprises an MST1 / 2 kinase inhibitor, a fibroblast growth factor 7, R-spondin 1, hydrocortisone, a B27 supplement, an N2 supplement, insulin, a dual regulatory protein, at least one Rho protein kinase inhibitor selected from Y27632, fasudil and H-1152, forskolin, gastrin, fetal bovine serum and cholera toxin. Compared with the existing culture method, the in-vitro culture using the culture medium has higher amplification efficiency; the culture of the gastric cancer organoid using the culture medium can maintain the morphological structure and pathological characteristics of the primary tissue, and improve the success rate and survival rate of the gastric cancer organoid culture.

The invention relates to a preparation method of a natural active molecule 6-acetyl forskolin. The preparation method comprises the following steps: by taking forskolin as a starting raw material, carrying out ester exchange on 7-acetyl and 6-OH to obtain isoforskolin, further selectively protecting 1-OH of isoforskolin, acetylating 7-OH, finally removing a protecting group of 1-OH, and carrying out reaction post-treatment and purification to obtain high-purity 6-acetyl forskolin. The synthesis method of the 6-acetyl forskolin comprises four steps of chemical reactions, the total yield is larger than 50%, the synthesis process is simple and stable, the steps are few, purification is easy and convenient, amplification production can be achieved, and an industrialized preparation method is provided for further development and utilization of the 6-acetyl forskolin.

The application discloses application of forskolin and / or isoforskolin in preparation of a medicine for preventing and / or treating colitis, and belongs to the technical field of medicines.The medicine can significantly improve the disease phenotype of colitis, including relieving weight loss, reducing disease activity index score, improving colon length shortening and histopathological damage.The mechanism of action involves multiple targets: repairing intestinal mucosal barrier function, reducing local inflammation of the colon, and regulating intestinal flora balance, restoring the abundance of beneficial bacteria and inhibiting the proliferation of pathogenic bacteria.The application provides a candidate drug with anti-inflammatory, barrier repair and flora regulation functions for the treatment of colitis, and expands the medical use of forskolin or isoforskolin.

The invention discloses an application of a small molecular medicine composition in transdifferentiation of glioma cells. The small molecular medicine composition comprises LiCl, Pirfenidone, Forskolin and RO4929097, and the small molecular medicine composition comprises LiCl, Pirfenidone, Forskolin and RO4929097. The invention further discloses application of the small molecule medicine composition in treating glioma, preparing a medicine for treating glioma and preparing a medicine for inducing glioma cells to be transdifferentiated into neuron-like cells. According to the present invention, with the application of the small molecule drug combination, the glioblastoma line U251 can be induced into the neuron-like cells, the tumor proliferation and the subcutaneous tumor formation ability can be inhibited, and the median lifetime of the xenotransplantation brain glioma model mouse can be prolonged.

Food supplement characterized by containing an active ingredient consisting of - 18 to 19 wt% green tea extract, of which 45 to 55 wt% of this extract consists of polyphenols and 6 to 7 wt% of this extract consists of theine, - 18 to 19% w / w extract from Cambodian Garcinia 10:1, - 14 to 15 wt% extract from red Moro orange 20:1, - 14 to 15 wt% extract from Gymnema sylvestre, of which 25 wt% of this extract consists of gymnemic acids, - 7 to 8 wt% extract of Coleus forskohlii, of which 5 wt% of this extract consists of forskolin, - 7 to 8% by weight berberine from Indian barberry, - 7 to 8% w / w extract from Paraguayan holly 10:1, - 7 to 8% w / w extract of fenugreek 10:1, - 3 to 4 wt% saffron extract, of which 0.1 to 0.2 wt% this extract consists of safranal, and max. 0.005 wt% chromium.

The invention belongs to the technical field of oral care, and particularly relates to an oral care composition and a preparation method thereof. The composition for oral care is prepared from the following components in percentage by mass: 0.1 to 0.3 percent of chitosan oligosaccharide, 0.08 to 0.14 percent of hail vegetable polysaccharide, 0.07 to 0.12 percent of forskolin, 0.05 to 0.09 percent of corn bud extract, 1 to 3 percent of thickening agent, 40 to 50 percent of humectant and the balance of water. The oral care composition can effectively inhibit the activity of glucosyltransferase, inhibit the generation of glucan, interfere the formation process of dental plaque, and reduce the attachment sites of pathogenic bacteria in the oral cavity. The oral mucosa epithelial cells can be promoted to proliferate and repair, and oral mucosa injury can be repaired. In addition, all the components in the composition are matched for use, so that oral microbial flora can be balanced, meanwhile, the effect of inhibiting pathogenic bacteria is achieved, and the oral environment is improved.

The present application relates to Foskolin induced differentiation treatment of hepatocellular carcinoma. Specifically, the present application relates to a method and use of Forskolin for inducing differentiation of human malignant hepatocellular carcinoma, thereby being applied to the treatment of malignant solid tumors. The research of the present application shows that after treating hepatocellular carcinoma cells with exogenous addition of Forskolin for a period of time, the hepatocellular carcinoma cells can be effectively induced to differentiate, thereby providing a new drug for tumor induced differentiation treatment. The purpose of the present application is to provide the use of Forskolin and its derivatives for anti-hepatoma drugs.

The application discloses a culture medium for constructing a liver cancer lung metastasis microtumor model and application thereof. The application relates to the technical field of biology, and provides a culture medium for constructing a liver cancer lung metastasis microtumor model, which is composed of three antibacterial and antifungal agents, HEPES, GlutaMax, human recombinant proteins EGF, HGF, IL-2, IL-15, R-spondin1, CXCL12, recombinant human lung surfactant proteins SP-A, SP-D, forskolin, A83-01, N-acetyl-L-cysteine, nicotinamide, N2 additives, B27 and a basic culture medium. The culture success rate of the liver cancer lung metastasis microtumor model is effectively improved.

The invention provides a method of promoting weight management in a mammal comprising administering to the mammal a composition comprising at least one weight loss agent and at least one mitochondrial enhancer. The weight loss agent can be selected from the group consisting of psyllium, guar gum, capsaicin, chitosan, caffeine, gamboge, mume, capsaicin, yohimbine, butterfly pea, glucomannan, African mango, guarana, pyruvate, carnitine, beta-glucan, fucoxanthin, raspberry ketone, white kidney bean, kola nut, chromium, ginseng, psyllium, St. John's wort, dandelion, hydroxycitric acid, conjugated linoleic acid, green tea, black tea, coffee bean extract, forskolin, and bitter orange. The mitochondrial enhancer can be selected from the group consisting of beetroot extract, nitrate, idebenone, nicotinamide riboside, elamipretide, vitamin C, vitamin D, vitamin E, thiamine, riboflavin, magnesium, calcium, phosphate, phospholipid, creatine, pyruvate, coenzyme Q10, NADH, niacin, L-carnitine, dichloroacetate, curcumin, schisandrin, and resveratrol. Compositions for weight management are also provided.

The present invention relates to a small molecule combination that can efficiently and rapidly induce the differentiation of malignant glioma cells into neurons and its application, and belongs to the field of biomedicine technology. The present invention is aimed at the current situation that differentiation therapy drugs have low therapeutic effects on glioblastoma. Through a large number of studies, it is found that a small molecule composition YFSS can effectively and rapidly induce GBM cells to differentiate into mature neuron-like cells. The composition YFSS includes Y27632, Forskolin, SB431542 and SP600125. At the same time, the present invention further develops a method for treating glioblastoma based on the small molecule composition, which continuously releases cerebrospinal fluid containing the composition YFSS to the tumor site, which can effectively treat GBM and improve its prognosis. The present invention provides a new way for the treatment of GBM.

Herein is reported a method for the production of one or more hamster-derived induced pluripotent stem cells comprising the steps of transducing a hamster cell with a Sendai virus comprising coding sequences for reprogramming factors, cultivating the transduced cell in the presence of at least a WNT pathway activator for about 12 days, replating the cells, adding a medium comprising one or more proteinogenic growth factors and cultivating the replated cells in the presence of the at least one WNT pathway activator, and selecting one or more cells that are positive for alkaline phosphatase and SSEA1 and that has stem cell morphology, wherein the at least one WNT pathway activator is in a small molecule cocktail comprising CHIR99021 (CHIR), 3-deazaneplanocin A (DZNep), forskolin, RepSox, tranylcypromine, aprotinoid acid (TTNPB), and valproic acid (VPA).

The invention provides a construction method and an application of recombinant yarrowia lipolytica for synthesizing furithrolin. The recombinant yarrowia lipolytica is obtained by introducing a combination of a coding gene of tenosyl ether synthase, a coding gene of cytochrome P450 oxidase, a coding gene of cytochrome P450 reductase, a coding gene of acetyltransferase, a coding gene of cytochrome b5 and a coding gene of scaffold protein, and knocking out a coding gene of phosphatidic acid phosphatase at the same time. The efficient synthesis of the fuchsin is realized. The construction method of the yarrowia lipolytica for biosynthesis of the eriolarynx element, provided by the invention, is simple to operate, and the constructed recombinant yarrowia lipolytica can efficiently produce the eriolarynx element and has relatively high production and application values.

The invention discloses a method for constructing a vascularized retina-like organ based on a vascular network. The method for constructing the vascularized retina-like organ comprises the following steps: carrying out multiplication culture on pluripotent stem cells until the cell confluence degree is 80-90%, and digesting the pluripotent stem cells; carrying out differentiation culture on the digested pluripotent stem cells in a vascular organ differentiation culture medium containing CHIR99021, bone morphogenetic protein 4, VEGF (vascular endothelial growth factor), Forskolin and bFGF (basic fibroblast growth factor) factors, so as to obtain vascular organs; carrying out differentiation culture on the digested pluripotent stem cells in a retina organ differentiation culture medium containing N2 and B27 retina induction factors to obtain retina organs; and co-culturing the retinal organ differentiated for 40-120 days and the vascular organ differentiated for 13-17 days, so as to obtain the vascularized retinal organ. According to the method, the in-vitro model of the retina is perfected, the blank that the retina organ lacks blood vessels is filled, and the method can be used for simulating the development process of the human retina and has wide application prospects.

The invention belongs to the field of biotechnology and biomedicine, and particularly relates to a mesenchymal stem cell exosome as well as an induction medium, a preparation method, a product and application thereof. The induction culture medium is prepared from didemethoxycurcumin, resveratrol and forskolin. The preparation method comprises the following steps: carrying out induced culture on adipose-derived mesenchymal stem cells by using the culture medium, collecting supernatant twice, and carrying out centrifugation, filtration and sucrose density gradient centrifugation purification to obtain the exosome. The mesenchymal stem cell exosome prepared by the method can be used as a core component to prepare medicines, can play an anti-fibrosis role in ways of inhibiting collagen synthesis, promoting repair of damaged alveolar epithelium, improving lung functions and the like, and has important application value in preparation of medicines for treating pulmonary fibrosis. A safe and effective novel strategy is provided for pulmonary fibrosis treatment, and clinical transformation of exosome therapy is promoted.

The invention relates to the field of cells, in particular to a composition for inducing autologous fibroblast reprogramming and application of the composition. According to the induction sequence, the composition comprises a first-stage small molecule compound, a second-stage small molecule compound and a third-stage protein molecule, and the first-stage small molecule compound is composed of Y27632, SAG, forskolin and Rapamycin; the second-stage small molecule compound is prepared from Y27632, SAG, forskolin, Rapamycin, AXL1717, RA and TNF-alpha (Tumor Necrosis Factor-alpha); the third-stage protein molecule is bFGF (basic fibroblast growth factor). The composition disclosed by the invention can be used for reprogramming fibroblasts so as to change the omics characteristics of the fibroblasts, so that the fibroblasts have enhanced immunoregulation, tissue repair and other biological functions related to treatment, and further safe, effective and unlimited autologous cell disease treatment is realized.

The invention provides a composition for reversing organ fibrosis and application of the composition, and belongs to the technical field of biological medicines.The cocktail composition is prepared from valproic acid, CHIR99021, Repsox, trans-benzene ring propylamine and forskolin. The HAT inhibition composition is prepared from A485 and WM-8014; the cocktail composition and the HAT inhibition composition are used independently or jointly, and hepatic fibrosis or pulmonary fibrosis can be reversed; when the composition is used in combination, a synergistic enhancement effect is shown in pulmonary fibrosis and hepatic fibrosis models, and the effects of improving fibrosis indexes and reversing epigenetic age are more remarkable; according to the composition provided by the invention, the epigenetic procedure of cells is accurately controlled, so that the cells are safely reversed from a pathological state to a healthy or resting state, the pathological phenotype of fibrosis is improved, the biological age of the cells is fundamentally reversed, and a major breakthrough in the field of fibrosis treatment is represented.

The present application relates to the technical field of stem cells, and particularly relates to a serum-free induction medium with clear components for promoting differentiation of induced mesenchymal stem cells into dermal papilla cells and an induction method. A small molecule compound composition comprises all-trans retinoic acid, forskolin, CHIR-99021 and minoxidil in a molar ratio of 0.2-5:2-50:0.2-10:0.2-10. The present application adopts multiple small molecule compounds to promote differentiation and function improvement of dermal papilla cells and transformation of mesenchymal stem cells into dermal papilla cells. The first three promote differentiation of dermal papilla cells, and the last one promotes proliferation and inhibits apoptosis of dermal papilla cells. The present application can solve the technical problems of high process cost and unsatisfactory clinical safety caused by the need to add cytokines in the process of inducing differentiation of stem cells into dermal papilla cells, and speeds up the induction process, and has an ideal application prospect.

The invention belongs to the technical field of organoid culture, discloses a culture medium for culturing a 3D breast cancer tumor organoid model and a model construction method, and particularly discloses a culture medium. Comprising an L-glutamine additive, an antibiotic, a Wnt signaling pathway activator, a bone morphogenetic protein inhibitor, a growth factor, a regulatory factor, a serum substitute, nicotinamide, acetylcysteine, hydrocortisone, estradiol, a eriochin, a TGF-beta signaling pathway inhibitor, a Rock inhibitor, a buffer solution and a basal culture medium. The invention provides the culture medium for culturing the breast cancer tumor organoid, and the culture medium can effectively simulate a tumor microenvironment, promote formation and growth of the breast cancer tumor organoid and ensure heterogeneity and genome stability of patient-derived tumors by adding specific growth factors, thereby laying a solid foundation for subsequent research and application.

The invention belongs to the technical field of polycystic kidney tissue organ culture, and particularly relates to a polycystic kidney disease model construction method and application of the polycystic kidney disease model in drug screening. Kidney pathological capsule wall tissue of an ADPKD patient is used as a cell source, the 3D organ of the polycystic kidney is constructed through the RGF culture medium, and the 3D organ has better gene stability and can better simulate in-vivo pathological characteristics of ADPKD. In order to enhance the vesicular state of the 3D organ and better simulate the physiological state of disease tissues, Forskolin is introduced, and the organ is applied to drug screening model construction. Through screening, the GSK2193874 has potential application in preparation of the medicine for treating the polycystic kidney disease. The culture mode is applied to in-vitro culture of ADPKD polycystic kidney organs, and is expected to be applied to disease mechanism research, drug screening, clinical cell therapy after correction and the like.

The invention discloses a composition for constructing an IOPN organ model, a model construction method and application. The composition for constructing the IOPN organoid model comprises an organoid model culture medium, and the organoid model culture medium comprises a penicillin-streptomycin double antibody solution, GlutaMAX, a serum-free supplement, Rspo-1, a Wnt activator, a growth factor, an HEPES buffer solution, N-acetyl-L-cysteine, a gastrin hormone, nicotinamide, forskolin, an ALK inhibitor, a ROCK inhibitor and the balance of a basic culture medium. The IOPN type organ model cultured by the composition for constructing the IOPN type organ model can maintain the original characteristics of tumors, provides a reliable biological model for pathological research and drug research and development of pancreatic tumors, and is convenient to operate and low in cost.

The application discloses a pharmaceutical-grade PDRN and a preparation method thereof, and belongs to the technical field of biotechnology. The method comprises the following steps: (1) grinding salmon testis tissue in a pre-cooled grinding buffer to obtain a homogenate; (2) using Forskolin to induce the grinding homogenate; the induction conditions are as follows: first, adding Forskolin to 8-12 muM, and standing for 1-3 hours at 4 DEG C; then, increasing the temperature to 10-20 DEG C, adding Forskolin to 18-22 muM and 2 mM glutathione, and treating for 2 hours; and (3) extracting the PDRN through SDS-sodium chloride.