65 results about "Functional reconstruction" patented technology

The invention relates to a biological material for repairing meniscus tear and a preparation method for the biological material. The material consists of an acellular matrix membrane on the inner layer, a porous structure collagen matrix membrane on the middle layer, a cell layer formed by cartilage cells on the outer layer and a cell sheet. The repair material has certain elasticity and toughness and is high in biological activity; the cartilage cells can secrete cell factors per se, and nutritional ingredients can be directly obtained through articular cavity synovia, and thus, the establishment of communication link between tissue cells at both ends of the tear during the meniscus tear process is accelerated; the immigration and the infusion of the cells in the material and autologous tissue cells are promoted; the tissue regeneration and functional reconstruction time is shortened; and the ideal novel material and the preparation method for the material are provided for clinically repairing the damage of the meniscus tear (including blood supply zones), especially the tissue regeneration and the functional reconstruction of no-blood zones and low-blood zones.

The present invention discloses an in-situ osteoplastic active calcium phosphate cement and its preparation process and application. Sodium alginate, collagen, hydrocellulose, polypeptide molecular beam, etc. are used to embed BMP, FGF, TGF-beta and other composite to maintain the activity of bioactive factor. Embedded bioactive factor is made to compound with CPC powder and mix with curing liquid to form paste capable of being moulded at will and self hardened in human body environment and humidity. Inside body, the bioactive factor can induce formation of blood vessel and new bone, formation of active tissue and functional reconstruction. The used CPC cured body may be used as calcium source and phosphorus source for forming new bone and speeding bone tissue formation. The present invention is applied for filling and repairing of bone.

The invention discloses a service robot controller and a control method thereof. The service robot controller adopts an open type secondary layered architecture and comprises a task planning layer and a motion control layer which are connected by virtue of a communication interface, wherein the task planning layer accepts tasks by virtue of a human-machine interface, carries out task planning and action sequence planning and adjusts an action sequence according to visual, hearing and proximity sense information obtained by a vision sensor and a hearing sensor; and the motion control layer carries out corresponding motion control according to a motion trail planned by the task planning layer and adjusts a motion control process in real time according to touch information obtained by a touch sensor. The service robot controller adopts an open architecture and provides a platform which flexibly defines and develops different function of the service robot. A layered open software-hardware platform can provide openness at different levels according to different requirements, and application requirements of the service robot on task reconfiguration and functional reconstruction can be met.

The invention provides a cylinder support for repairing spinal cord injury. The cylinder support comprises a collagen sponge cylinder support body. An epidermal growth factor receptor antibody cetuximab is crosslinked with the collagen sponge cylinder support body. Neural stem cells are loaded through the collagen sponge cylinder support body crosslinked with the epidermal growth factor receptor antibody cetuximab so as to promote transplanted neural stem cells to be differentiated into neurons, and the cylinder support which facilitates injured spinal nerve regeneration and functional reconstruction is constructed. The collagen sponge cylinder support body has ultrahigh porosity and provides sufficient space for cell growth. More importantly, controlled-release of the antibody cetuximab can relieve effect myelin protein in a microenvironment after spinal cord injury, promotes transplanted neural stem cells to be differentiated into neurons, restrains transplanted neural stem cells from being differentiated into astroglia cells, and also promotes growth of nerve axons so as to facilitate injured spinal nerve regeneration and functional reconstruction. In addition, the invention further provides an application method of the cylinder support for repairing spinal cord injury.

The invention discloses a bioactive ceramic fiber composite scaffold for bone repair and a preparation method of the composite scaffold, and relates to the field of bone repair materials. In order toovercome the shortcomings of large brittleness, difficulty in processing, unsatisfactory degradability and biological activity and the like of a traditional ceramic scaffold material, bioactive ceramics such as calcium silicate serve as a scaffold body, and the surfaces of ceramic fibers are uniformly coated with a layer of degradable biomedical high polymer materials by an impregnation-centrifugation-drying process to obtain a high-porosity, good-mechanical-property and biodegradable bioactive ceramic fiber composite scaffold material for bone repair. The bioactive ceramic materials are easily subjected to synostosis with host bones, inorganic ions released by degrading the bioactive ceramic materials can participate in and promote osteogenesis and even angiogenesis metabolism activity ofa body and have irritating or inducing functions for tissue regeneration repair, and defective bone tissue repair and functional reconstruction are promoted. Degradation products of the biomedical high polymer materials on the surface of the scaffold are harmless and can be excreted to the outside of the body by metabolism.

The invention provides an implant and a method for manufacturing a mandibular implant with PEKK (polyetherketoneketone) supporting and fixing units and a tissue engineering growth unit. The method includes steps: 1) image acquisition and three-dimensional model establishment; 2) molding unit and fixing unit design; 3) supporting unit design; 4) growth unit design; 5) individual composite-structure implant assembly; 6) individual composite-structure implant manufacturing; 7) in-vitro culture. A molding unit, fixing units, supporting units and the growth unit jointly form an individual composite-structure implant, the supporting units, the fixing units and the molding unit are made of PEKK materials and capable of providing sufficient mechanical strength to keep structural integrity from occlusion functional reconstruction, and the growth unit which is an internally degradable stent is capable of guiding new bone generation.

The invention discloses a method for establishing a human bladder transitional cell carcinoma cell line and a mouse model of bladder carcinoma tissue recombination functional reconstruction. The method includes the primary cell culture, immortalized cell line identification, tissue reorganization and planting, HE staining, and immunohistochemistry. The invention establishes a novel stable human bladder transitional cell carcinoma cell line, and uses the cell line to construct the mouse model of bladder carcinoma tissue recombination functional reconstruction. The occurrence and development of bladder carcinoma are simulated in mice. The method researches on the biological behavior of bladder carcinoma from in vitro molecular and cell biology and in vivo animal models, and provides an effective tool for the discovery of new biomarkers and therapeutic measures.

The invention relates to a co-culture method of photosensory precursor cells and retinal tissue in vitro and aims to build a co-culture system for the photosensory precursor cells and denaturation retinal tissue of retina photoreceptor cells. The method comprises the following steps: a photosensory precursor cell layer of an embryonic eye source is prepared; a nerve cell layer of a denatured retina is prepared; the nerve cell layer of the denatured retina is placed on the photosensory precursor cell layer; an epithelial layer of retinochrome is prepared in the lower chamber of a plug-in type tissue culture dish; the co-culture system of the retinal tissue and cells is built in vitro, and the retinal tissue and cells are cultured in an incubator with 5% CO2 at the temperature of 37 DEG C. By adopting the method provided by the invention, the biological characteristics and cellular structure of host cells and transplanted cells, the synaptic contact of the transplanted cells and the host cells, and the chromatin conformation reconstruction of the transplanted cells and the host cells can be observed and detected directly in vitro, and the traces of survival, proliferation, differentiation and functional reconstruction of photosensory precursor cells of a transplanted embryonic eye source can be tracked.

The invention discloses a growth-factor-containing nanofibre porous composite material capable of repairing a bone and a preparation method thereof. The preparation method comprises the following steps of: dissolving chitosan in acetic acid; adding bone growth factors into the solution, and uniformly mixing the solution; immersing a porous inorganic material capable of repairing the bone in the solution under a negative pressure for 1-2 hours, and taking out the material; and freezing the porous material capable of repairing the bone at a low temperature, lyophilizing the material, immersing the material in a stabilizer solution to stabilize the material, washing the material with water, lyophilizing the material again, and sterilizing the material to prepare the growth-factor-containing nanofibre porous composite material capable of repairing the bone. In the invention, the growth-factor-containing nanofibre porous composite material capable of repairing the bone simulates the nanofibre structure of an extracellular matrix of the bone; the bioactivity of the bone growth factors can be kept, and the bone growth factors can be released continuously and slowly; the growth-factor-containing nanofibre porous composite material is beneficial for the regeneration and functional reconstruction of bone tissues; and the defect that traditional slow-release systems of the bone growth factors cannot better simulate a physiological microenvironment required for the regeneration of the bone tissues is overcome.

The invention discloses a hernia bioremediation mesh and a preparation method thereof. The hernia bioremediation mesh is made of animal tissues, immune ingredients such as cells and DNA are removed, and the animal tissues are cut into thin strips, twisted into threads, and woven into the bioremediation mesh. Xenogeneic decellularized materials obtain greater intensity through twisting into the threads, so that the xenogeneic decellularized materials are not prone to breaking; since the threads are interwoven with each other after weaving, external force of tensile tearing does not act on one point but on a plurality of interwoven points, so that the mechanical properties are good; and the defects that the xenogeneic decellularized material hernia bioremediation mesh has poor mechanical intensity and needs to be introduced with cross-linking agents or synthetic materials are overcome. The structure of the mesh is prepared by a weaving process, and the size of a mesh aperture can be easily adjusted through weaving parameters, so that requirements of practical application are met. Meanwhile, the hernia bioremediation mesh retains an original three-dimensional structure, non-collagen proteins, growth factors and other components of an extracellular matrix, so that the functional reconstruction and postoperative healing of the tissues are promoted.

The present invention relates to follicular stem cell originated from human hair and its amplifying preparation process, and belongs to the field of medical preparation and human cell culture technology. The present invention adopts improved two-step protease digesting method to amplify follicular stem cell originated from human hair and obtains follicular stem cell with active expression of beta-integrin and keratin 19 in an optimized follicular stem cell preparing environment. The optimized micro environment endows follicular stem cell with fast amplification, antisenile characteristic, the epidermic cell-like, fibroblst-like and hair bulb tissue-like structure or functional expression. The present invention makes it possible to provide sufficient seed cell sources for the fast repair and functional reconstruction of skin defect.

The invention relates to a dynamic overloading method for a DSP (Digital Signal Processor). The method comprises the following steps: 1, generating mapping files; 2, initializing the DSP; 3, guiding and copying overloading kernels in the mapping files into an internal storage of the DSP so as to cover primary application programs through overloading interface functions; 4, guiding and copying application programs in the mapping files which are stored in an external storage space into the internal storage of the DSP so as to cover the overloading kernels through the overloading kernels in the internal storage, and generating application programs to be operated; and 5, executing the application programs to be operated so as to finish dynamic overloading. As the traditional boot loading mode of an embedded type signal processing ADSP-TS20xS serial processor hardware platform cannot satisfy requirements of system functional reconstruction, the dynamic overloading method is additionally provided with the overloading kernels, and can use the overloading kernels to realize the real-time dynamic overloading operation for program mapping, so that the reconstruction ability of an embedded system is improved.

The invention discloses an ultra wide band reconfigurable receiving and transmitting front end and a signal receiving and transmitting method, and belongs to the technical field of communication. Thetransmitting-receiving front end comprises a reconfigurable frequency converter. According to the reconfigurable intermediate frequency link and the reconfigurable zero intermediate frequency transceiver, a superheterodyne frequency conversion scheme is combined with a zero intermediate frequency conversion scheme, a two-way amplifier, a reconfigurable band-pass filter, a reconfigurable low-pass amplifier, a numerical control attenuator and a variable gain amplifier are adopted, and a zero intermediate frequency receiving and transmitting self-closed loop technology is combined, so that 3GHz-can be realized; And transmitting and receiving conversion from the 18GHz radio frequency signal to the baseband signal. Functional reconstruction of receiving and transmitting, frequency conversion architecture and the like and reconstruction of technical indexes of gain, instantaneous bandwidth and the like are achieved, and the method is particularly suitable for being used in software radio andcognitive wireless receiving and transmitting systems in the communication field.

The invention provides a method for reprogramming spinal marrow astrocytes into motor neurons through induction. The method comprises steps as follows: (1) primary rat astrocytes are subjected to pure culture; (2) 7 small molecule drugs including SB431542, LDN-193189, RA, bFGF, Purmorphamine, Forskolin and VPA are selected according to substances required in the stages of neuron induction and motor neuron formation and development, and the astrocytes are induced and reprogrammed in vitro through the small molecule drugs; (3) the reprogramming and differentiation conditions of the astrocytes to the motor neurons are analyzed through observation of morphologic change of cells and methods of chemical technology analysis of immunofluorescent cells, real-time fluorescent quantitative PCR analysis and statistical analysis. The rat astrocytes can be successfully induced and reprogrammed into the motor neutrons in vitro through small molecular drug composition, and the transdifferentiation exceeds 75%; in-situ direct reprogramming of the astrocytes to the neurons is a possibly comparatively feasible thought in SCI (spinal cord injury) repairing and functional reconstruction treatment.

The invention relates to an icariin and hyaluronic acid composition, a method for preparing the same and application of the icariin and hyaluronic acid composition. The icariin and hyaluronic acid composition, the method and the application have the advantages that the icariin and hyaluronic acid composition can be used for preparing medicines for treating cartilage diseases and can be particularly used for preparing injection for joint cavities, accordingly, the shortcoming of limitation of single hyaluronic acid treatment can be overcome, the application range can be expanded, and adverse reaction can be reduced; effects of covering and protecting the surfaces of cartilage can be enhanced by the aid of pharmacological effects of icariin on cartilage cells; the icariin and hyaluronic acid composition can act on synovial cells, synthesis of autologous macromolecular hyaluronic acid can be promoted, and accordingly the purposes of nourishing the cartilage, lubricating the joint cavities, relieving pain, improving the daily activity ranges and the joint activity ranges of patients and the like can be achieved; the icariin and hyaluronic acid composition can act on the cartilage cells, accordingly, the viability of the cartilage cells can be enhanced, secretion of active substances such as type II collagen of the cartilage cells and mucopolysaccharide can be promoted, and nourishing, lubricating, regenerating and functional reconstruction effects and the like can be jointly realized; knowledge on traditional Chinese medicine injection can be changed, the adverse reaction can be reduced to a great extent, curative effects of hyaluronic acid and the traditional Chinese medicine injection can be improved, and the application ranges of the hyaluronic acid and the Chinese medicine injection can be expanded.

The invention discloses a reconstructible wireless information network system structure and a reconstruction realization method thereof; the network system structure comprises a flexible node module,a data center module, a user center module, a web master and reconstruction center and a target management center; the reconstruction realization method comprises the following steps: multi-mode capture tracking based on a task plan, a target mark, and service demands; target classification based on target features; network reconstruction management based on the task plan, the target features andthe service information; flexible node functional reconstruction management based on target types. The structure and method can provide various types of information services for users through networkreconstruction and function reconstruction.

The invention discloses a NGF monoshell-multinuclear microsphere/PCL nanofiber catheter which can promote regeneration of peripheral nerves, govern functional reconstruction of target muscles and better promote recovery of motor function of peripheral nerves more quickly. The invention also discloses a preparation method of the NGF monoshell-multinuclear microsphere/PCL nanofiber catheter. The preparation method comprises the following steps: first, preparing NGF buried PLGA microspheres; then preparing NFG-buried monoshell-multinuclear microsphere and a polycaproloactone PCL nano fiber nervehollow catheter; and finally, dissolving the prepared NFG-buried monoshell-multinuclear microspheres in a PBS solution and injecting the solution into the polycaproloactone PCL nano fiber nerve hollowcatheter to obtain the NGF monoshell-multinuclear microsphere/PCL nanofiber catheter.

The invention discloses an application of a polypyrimidine sequence binding protein silencing agent combined with retinoic acid and purinine amine in preparation of a medicine for repairing spinal cord injury, and belongs to the technical field of biological medicine. The polypyrimidine sequence binding protein (PTB) is silenced in vitro through a virus, and meanwhile, micromolecule retinoic acid (RA) and purinol amine (PMA) which are related to motor neuron differentiation are jointly added, so that mouse spinal cord reactive astrocytes are successfully reprogrammed into motor neuron; and help is provided for further in-vivo research on the effect of a PTB combined micromolecule reprogramming strategy in spinal cord injury post-repair, so that better spinal cord injury repair and function reconstruction effects are realized.

The invention discloses a nutritive bowl for field planting of leymus chinensis in deteriorated grassland. The nutritive bowl comprises nutritive bowl soil, wherein a brassinosteroid aqueous solution,formulation complex fertilizer and pregelatinized starch as a soiling bonding and nutritive bowl setting substance are added in the nutritive bowl soil; the success rate of field planting and expanding propagation of the leymus chinensis can be effectively improved, centralized management at seedling stage is facilitated and the utilization rate of the seeds and the survival rate of seedlings areimproved; rapid planting of the leymus chinensis in the deteriorated grassland and functional reconstruction of an ecological system are promoted; sufficient nutrients of the nutritive bowl can meetthe demands of field planting and expanding propagation of the leymus chinensis; once a cultivated nutritive bowl is transplanted to the deteriorated grassland, a large grassy field can be quickly restored by means of the advantages of colonizing division and integration of the leymus chinensis; by adopting the pregelatinized starch as a bonding and setting substance, the nutritive bowl is not easy to loosen or disintegrate, and the seedling stage management, movement, transportation and transplanting are facilitated.