60 results about "Biological repair" patented technology

The invention discloses a method for managing town black and olid river pollution in situ by utilizing biological repair technology, which takes the steps and measures of aerating and oxygenizing in riverway, adding a complex microbial agent, biologically repairing bottom sediment, arranging a bio-nest system and the like, so as to control and eliminate the exogenous and endogenous pollution of the river, purify the water quality of the river, improve the self-purification ability of the water body and achieve the purpose of eliminating black and olid. The method does not need to build structures in the riverway or lead the river water out of the riverway to be treated, or dredge the bottom sediment in the river, therefore the method is generally applicable to incomplete sewage interception and dredging or the rivers which can not be intercepted sewage or dredged temporarily, and the in situ treatment to the riverway which is affected by tide and has complicated fluid variation. Therefore, the method which can lead the water body to recover normal ecological function provides the practical, economic and convenient treatment method for various town black and olid rivers.

Methods and compositions for the biological repair of cartilage using a hybrid construct combining both an inert structure and living core are described. The inert structure is intended to act not only as a delivery system to feed and grow a living, core component, but also as an inducer of cell differentiation. The inert structure comprises concentric internal and external and inflatable / expandable balloon-like bio-polymers. The living core comprises the cell-matrix construct comprised of HDFs, for example, seeded in a scaffold. The method comprises surgically removing a damaged cartilage from a patient and inserting the hybrid construct into the cavity generated after the foregoing surgical intervention. The balloons of the inert structure are successively inflated within the target area, such as a joint, for example. Also disclosed herein are methods for growing and differentiating human fibroblasts into chondrocyte-like cells via mechanical strain.

The invention discloses a method for preparing and using a microbial immobilized material for repairing contaminated soil. The method comprises the following steps: (1) after being dried by air, the waste biomass raw material is pulverized or ground into 1-20mm of particles; (2) the biomass particles are carbonized and sterilized at the temperature of 150-450 DEG C and under limited oxygen condition for 0.5-6 hours to prepare a carrier material; (3) the carrier material is put into bacterium suspension for enrichment culture according to the solid-to-liquid ratio of 0.5-2mL / g, then the microbial immobilized material is produced; (4) the produced microbial immobilized material is added to organic contaminated soil according to the proportion of 1-500 tons per hectare soil, 0-30cm of topsoil is ploughed to cause the microbial immobilized material to be mixed evenly in the soil, simultaneously, the contaminated soil is biologically repaired and safe agricultural products are produced. The method has the advantages that the carrier material contains abundant lignin structures, carbon and nutrient elements, which provides more suitable matrix for microbial growth; and the immobilized material has strong affinity, high immobilization efficiency, strong adsorption capacity and good resistance against the pollutants in the environment; and the immobilized material is a soil fertilizer, and is suitable for large-scale in-situ biological repair of the contaminated soil.

A method for treating a facet pain in a subject by providing an effective amount of therapeutic agent in manner that by a biological repair effect on the facet is produced as compared to a temporary anti-inflammatory effect.

The invention provides a biological repair tablet for herniae and a preparation method thereof. The repair tablet uses small intestine submucosa tissues of inbred line animals without cell and DNA (deoxyribonucleic acid) components as the raw material, completely reserves the extracellular matrix component and structure, and has a micropore structure. The preparation method of the repair tablet comprises the following operation steps: determination of animal source, pretreatment and rough cleaning of small intestine tissues, virus inactivation, cell removal, DNA removal treatment, formation, packaging and sterilization. The biological repair tablet for herniae prepared by the method uses an inbred line animal as an animal source, and thus, the hereditary features are pure, stable and uniform, thereby radically ensuring the stability and uniformity of different batches of products; and the biological repair tablet for herniae has fewer animal source DNA residues, completely reserves the three-dimensional structure of natural ECM, and has the advantages of low immune source property and high infection resistance.

The invention provides a biological repair membrane and a preparation method thereof. In the prior art, the existing biological repair membrane has the following disadvantages that the mechanical strength is poor, the suture point has the cerebrospinal fluid leakage risk, the tissue regeneration and repair promoting performance is weakened, the residual toxicity exists after the degradation, the biocompatibility is poor, and the adhesion between the repair material and the brain tissue is easily caused, and other complications are easily induced. According to the present invention, the animal-derived membrane tissue is subjected to cell removing and antigen removing, and a natural polymer material coating capable of being adsorbed and providing bleeding stopping effect is coated on the treated animal-derived membrane tissue so as to solve the problems in the prior art; the method specifically comprises: pretreatment, degreasing with an organic reagent, cell removing and antigen removing with an alkali liquid, coating preparation, vacuum compression and freeze-drying, packaging, and sterilization so as to complete the preparation of the biological repair membrane; and the biological repair membrane has advantages of bleeding stopping, antibacterial property, adhesion, natural three-dimensional space structure retaining, good mechanical property, good biocompatibility, good tissue growth induction, degradation non-toxicity, wide raw material source, low price, and easy mass-production.

The invention provides a material for promoting the healing of operative wounds. The material is a natural biological repair material obtained by subjecting a flake-shaped or membrane-shaped soft tissue or a derivative thereof coming from humans and / or animals to a series of physical, chemical or biological treatment. The biological repair material can be presented as solid or liquid, is fixed onthe operation wounds through a suturing, attaching, smearing or injecting and other surgical method, plays a role in promoting the wounds to heal in a wound healing process, and is convenient, safe and rapid to use. Compared with the conventional operative wound healing material, the material has the advantages of promoting the speed and effect of repairing wounds, preventing wound infection and reducing operation scars after the wounds healing.

The present invention discloses a soil biological repair method, which comprises (1) investigation analysis, (2) flora culture, (3) nutrient solution preparation, (4) biological surfactant preparation, (5) flora pouring, (6) full load repair, and (7) purified soil repair evaluation. According to the present invention, the capsule type bacterial powder is used, can complete the same biological repair work within 2-3 months compared with the 1 year period of the traditional process, and can be directly used for repairing high concentration heavy oil contaminated soil, the oil film and the oil odor can be rapidly inhibited, the automatic repair degree is high, and frequent soil plowing is not required.

The present invention belongs to the field of environment protecting technology, and is especially soil repairing electric and biological electrode matrix method. The present invention features that one dimensional or two dimensional electrode matrix is adopted to disperse foreign matter into soil in monoway running mode and to strengthen the mass transfer between the pollutant and the degrading bacteria and complete in-situ reinforcement in bi-directional mode. The present invention can enrich the pollutant in soil to specific treating area, disperse the nutritive matter and microbe injected in fixed point to soil fast, reinforce the mass transfer between the pollutant and the degrading bacteria and raise the biological repair efficiency.

The invention discloses a method for screening dissimilatory Fe(III)-reducing bacteria. The method comprises the following steps: (1) carrying out enrichment culture on sediment sludge by way of gradually improving the Fe(III) concentration in a liquid culture medium; and (2) separating and purifying the dissimilatory Fe(III)-reducing bacteria from enriched flora and carrying out gradient-acclimation on heavy metal endurance capacity and salt tolerance. The method for screening dissimilatory Fe(III)-reducing bacteria is simple and feasible and short in screening period. In the screening process, expensive separating equipment such as an anaerobic operation system and the like is required, so that the screening cost is effectively lowered. The obtained pure strain has a relatively strong Fe(III) reducing capacity. In addition, through gradient-acclimation on heavy metal endurance capacity and salt tolerance of the pure strain, a dissimilatory Fe(III)-reducing bacterial strain which has the endurance capacity to Cr (VI) heavy metals and different salt concentration environments can be quickly and simply obtained, thereby providing a germplasm resource for biological repair in the environments such as a heavy metal region, a salt region and the like.

Rice is the main crop in china, and the rice growing is the first farming. However, with the water consumption accounting for a half of national freshwater resource, the rice is the first water consumer, and the water utilization ratio is low. The phenomenon is described as one kilo of grain requiring one ton of water. Since the 1970s, the rice growing has been a typical example of agricultural non-point source pollution because of high production pursuance, and luxurious and random application of fertilizers and pesticides. For the food and etiological security, the rice growing must be reformed. With the reform of rice growing technology as a target, an ''irrigation paddy 'wheat planting type' high rice production wet planting method'' is invented. The invention comprises: (1) a soil fertility biological repair technology which integrates the soil utilization with the maintenance of soil fertility, with the paddy fertilization and crop rotation as the basis, (2) a direct sowing technology with seeding keeping, weeding and falling prevention technology as the basis, and (3) a novel water-saving rice growing irrigating system, that is, seeding in the wet soil, cultivating the root in a dry tube, developing ear in the ditch water and preventing the weakness in the dry and the wet environment, with physiological water requirement of the rice as the basis. The invention has the specific effects of saving the cost by 10 percent, saving the water by 40 percent, and achieving the stable production of 500Kg / mu, and has other ecological benefits.

The invention provides a biological repair tablet for endocranium and a preparation method thereof. The repair tablet uses small intestine submucosa tissues of inbred line animals without cell and DNA (deoxyribonucleic acid) components as the raw material, completely reserves the extracellular matrix component and structure, and has a micropore structure. The preparation method of the repair tablet comprises the following operation steps: determination of animal source, pretreatment and rough cleaning of small intestine tissues, virus inactivation, cell removal, DNA removal treatment, formation, packaging and sterilization. The biological repair tablet for endocranium prepared by the method uses an inbred line animal as an animal source, and thus, the hereditary features are pure, stable and uniform, thereby radically ensuring the stability and uniformity of different batches of products; and the biological repair tablet for endocranium has fewer animal source DNA residues, completely reserves the three-dimensional structure of natural ECM, and has the advantages of low immune source property and high infection resistance.

The invention relates to a method for preparing a soil composite biological repairing agent by using maize straws and the application of the repairing agent in soil property improvement. According to the method, maize straws which are low in cost and renewable are adopted as raw materials, treated trichodermahamatums T22 and T8-2 are taken as strains, and then liquid-liquid two-phase mixed strainfermentation technology is carried out to prepare the soil composite biological repairing agent. The repairing agent has a good repairing effect on soil, a good degradation effect on pesticide residues in soil, a strong restraining or killing effect on soil spreading pathogenic bacteria and a promoting effect on crop growth and is harmless to ecological environment.

The present invention relates to the field of medical technology. By using carboxymerhyl chitosan as slow-releasing assistant and antiseptic as carrier and through emulsification and crosslinking process, a kind of degradable slow-releasing microball is prepared capable of being implanted in operation incision to prevnet post-operational local infection. The slow-releasing preparation of the present invention has the features of degradability and small volume, and avoids the shield to biological repair in oganism.

The invention relates to a preparation method of an organic functional fertilizer containing a PAL biological repairing preparation, and belongs to the technical field of fertilizers. The preparation method comprises the following steps of step I, taking lignite, drying the taken lignite, crushing the dried lignite, screening the crushed lignite, taking plant ash powder and fish meal, uniformly mixing the taken plant ash powder and the taken fish meal with the lignite so as to obtain powder, adding water to the powder in the proportion of the power to water being 1 to 10, putting the water and the powder in a pot for boiling over for 15-20 minutes, and then performing cooling so as to obtain a stock solution A and residues A; and step II, drying pig manure, taking a PAL porous composite, crushing the taken PAL porous composite, screening the crushed PAL porous composite, uniformly mixing the screened PAL porous composite with lumbricus, crop straw, vinasse, vinegar dregs, eggshells, the residues A and the pig manure, adding probiotics and the stock solution A during stirring, so that all of the materials are uniformly mixed to form a mixture, fermenting the prepared mixture under a certain condition, extruding the mixture fermented in the step II, performing granulation and performing packaging, so as to obtain the organic functional fertilizer. Adsorptivity combination can be formed, relative-polymeric structure beneficial ingredients are easy to absorb by crops, and have favorable release control effects, and the crops can be adjusted to uniformly grow, so that the quality of the crops is improved.

The invention belongs to the technical field of genetic engineering and specifically relates to an OsGCW14 gene promoter which is used for regulating and controlling specific expression of exogenous genes of rice in roots and an application thereof. The invention discloses a rice gene promoter, and the nucleotide sequence of the rice gene promoter is as shown in SEQ (sequence) ID (identity) No.1 (number 1). The invention further introduces the application of the OsGCW14 gene promoter for constructing a transgenic plant carrier with root specific expression and transforming a plant host. The OsGCW14 gene promoter has the advantages that the OsGCW14 gene promoter can perform biological repair on soil pollution, or provide drought resistance and salt and alkali tolerance of plants, or improve high-efficiency absorption of nutrients in the soil of the plants, or enhance the resistance to pathogenic microorganisms in the soil and the like, and simultaneously transform the architecture of root systems of the plants for performing genetic improvement on crops.

The invention discloses a metallic cadmium resistance associated protein KdpD and a coding gene and application thereof. The amino acid sequence of the metallic cadmium resistance associated protein KdpD is shown as SEQ ID NO: 1; and the coding gene of the metallic cadmium resistance associated protein KdpD is shown as SEQ ID NO: 2. Escherichia coli transformation experiments prove that the expression of the gene in escherichia coli can improve the resistance of the gene to heavy metallic cadmium. The KdpD coding gene can be used for improving the heavy metallic cadmium resistance of microbes and plants, is further used for clearing heavy metal pollution in the environment, and provides a biological resource with excellent performance for biological repair.

The invention discloses a medical biological yarn, a medical biological repair mesh and a preparation method thereof. The medical biological yarn can be woven into the repair mesh by taking tissues ofan animal as a raw material, removing immune components such as cells and DNA and slicing the raw material into strips and twisting the strips to the yarn. A special cell-removal material is twistedto yarns to obtain greater strength, so that the yarns are hard to break. The yarns are interwoven after being woven, a stretching and tearing external force is not applied to one point but to multiple interwoven points, so that the yarn has good mechanical property. The defect that the mechanical strength of the biological repair mesh is poor and a crosslinking agent or a synthetic material is needed is overcome. Moreover, the structure of the mesh is prepared from a weaving process. The size of the pore diameter of the mesh can be adjusted easily by means of weaving parameters to meet the demand of actual applications. In addition, the medical biological yarn keeps original components such as three-dimensional structures, collagenous fibers, non-collagenous fibers and growth factors in an extracellular matrix and promotes functional reconstruction and postoperation reunion of tissues.

The invention discloses bacillus siamensis capable of being used for repairing concrete cracks, a microbial inoculum and application. The bacillus siamensis is named as bacillus siamensis, the strainnumber of the bacillus siamensis is ZJB20040, and the preservation number of the bacillus siamensis is CCTCC NO: M 2020276. The bacillus siamensis ZJB20040 provided by the invention has a preservationnumber of CCTCC NO: M2020276, has very good thermal stability and acid-base stability, can adapt to environments with different pH values when being used for repairing concrete cracks, and has good potential and application prospect when being used for preparing a biological repairing preparation for self-repairing the concrete cracks.

The invention relates to biological repair of a phosphorus polluted water body, in particular to a method for repairing a phosphorus polluted water body by using polygonum hydropiper. The method for repairing the phosphorus polluted water body by using the polygonum hydropiper comprises the following steps of: planting the polygonum hydropiper into the phosphorus polluted water body polluted by livestock and poultry excrements, wherein a direct transplanting or direct seed sowing method is adopted when the polygonum hydropiper is planted; and periodically harvesting the planted polygonum hydropiper, wherein the harvested polygonum hydropiper is used as a green manure and returned to the soil. The method has the advantages of low cost, good treatment effect, strong operability and the like.

The invention relates to the technical field of biological medical engineering, and discloses a biological repairing material for an abdominal wall defect and a preparation method of the biological repairing material. The method comprises the following steps: (1) performing pre-treatment on the muscular tissue of rectus abdominis of a dead animal; (2) performing cell extraction treatment to prepare a skeletal muscle acellular matrix biological thin sheet; (3) dissolving the skeletal muscle acellular matrix biological thin sheet into pepsase for digestion; (4) performing electrostatic spinning on skeletal muscle acellular matrix microparticles obtained by digestion and polycaprolactone to obtain a polycaprolactone / skeletal muscle acellular matrix blended electrostatic spinning fiber film which is the biological repairing material disclosed by the invention. According to the method, a skeletal muscle acellular matrix from abdominal wall tissue and polycaprolactone are combined by an electrostatic spinning technology; the preparation materials can be degraded, and products from degradation are harmless to a human body; the prepared biological repairing material can well simulate constituents and the structure of an extracellular matrix and is good in biocompatibility and biomechanical property.

An object of the present invention is to provide a means for improving the affinity of an in vivo embedded material such as an artificial blood vessel or an artificial dura mater with a tissue adhesive, so as to quickly prevent the leakage of the blood or spinal fluid. The present invention provides a polymeric material used in combination with a tissue adhesive, which comprises carbon or silicon as a constitutional element, and at least a portion of the surface of which is modified by ion bombardment.

The invention provides a multi-effect repairing material for repairing a black and odorous water body as well as a preparation method and application thereof. The multi-effect repairing material is prepared from the following components in parts by mass: 15 to 30 parts of oxygen releasing material, 15 to 35 parts of natural porous alpha-MnO2 type material particles, 7 to 12 parts of additive, 12 to 23 parts of biological charcoal catalyst, 5 to 18 parts of modified ultrafine titanium dioxide, 8 to 25 parts of waste metal filing and 5 to 12 parts of pH (Potential of Hydrogen) buffering agent. The multi-effect repairing material provided by the invention has stable properties, nano-pores and a complicated network structure, and has relatively good adsorption and catalytic oxidation activity;the multi-effect repairing material has long oxygen releasing lasting time and high releasing efficiency and can ensure the sustainability of an underwater biological repairing effect; a ternary internal electrolysis system can be automatically formed in a water body and multi-effect cooperation of adsorption, chemical oxidization, catalytic oxidation, electric oxidation and biological repairingis realized.

The invention discloses a biological repair sheet. The biological repair sheet comprises a middle layer providing a mechanical support for the biological repair sheet, an anti-adhesion layer which is connected to the middle layer, is located at one side of the middle layer and is used for preventing tissue and organ adhesion, and a biological regeneration material layer which is connected to the middle layer, is located at the other side of the middle layer and is used for promoting soft tissue regeneration and inhibiting scars. The invention also discloses a preparation method of the biological repair sheet. The biological repair sheet has physical inlaying effects so that all the layers of the biological repair sheet are bonded tightly and are difficultly separated, the last step of hot pressing for strengthening is avoided and heating-caused biodegradable polyester material degradation is avoided. A sponge structure is formed through a freeze-drying technology so that the biological repair sheet is soft. The biological repair sheet has good softness.

The invention relates to a biological periosteum repair material and a preparation method thereof, and particularly relates to an acellular dermis matrix material and a preparation method and application thereof to periosteum repair. The preparation method comprises: (1) taking a jejunum segment of a pig small intestine, cleaning up, radially cutting open, removing a mucous layer, a myolemma layerand a serosal layer and obtaining a pig small-intestine mucous lower-layer tissue membrane; (2) sequentially carrying out the following processing on the obtained pig small-intestine mucous lower-layer tissue membrane: firstly, placing in peracetic acid solution to soak and cleaning up; then placing in sodium hydroxide solution to soak and cleaning up; finally, placing in acetone solution to soak, cleaning and obtaining a pig small-intestine mucous lower-layer tissue acellular matrix. The biological periosteum repair material is high in biological safety, low in immunogenicity and cytotoxicity, good in biocompatibility, can promote multiplication of cells adhered to the biological periosteum repair material, is applicable to use as a biological repair material and is particularly suitablefor periosteum repair.

An object of the present invention is to provide a means for improving the affinity of an in vivo embedded material such as an artificial blood vessel or an artificial dura mater with a tissue adhesive, so as to quickly prevent the leakage of the blood or spinal fluid. The present invention provides a polymeric material used in combination with a tissue adhesive, which comprises carbon or silicon as a constitutional element, and at least a portion of the surface of which is modified by ion bombardment.

The invention relates to a specific gene segment suitable for real-time fluorescent quantitative PCR detection of pseudomonas PPZ-1 as well as primers of the specific gene segment and applications of the primers. For the specific gene segment suitable for real-time fluorescent quantitative PCR detection of pseudomonas PPZ-1, the nucleotide sequence is as shown in SEQ ID NO.1; the invention further relates to detection primers designed by utilizing the specific gene segment and applications of the detection primers. According to the technical scheme provided by the invention, the distribution of pseudomonas PPZ-1 can be tracked in real time, and thus the demand of biological repair monitoring is satisfied; according to actual detection, background noises can not be detected in a polluted sample in which pseudomonas PPZ-1 is not fed.

The invention relates to a technology for applying transgenic plants to repair polluted water bodies. A target plasmid p3301-121-ZnT1 is obtained by chemical synthesis of the published ZnT1 gene sequence related to heavy metal tolerance, the agrobacterium containing the target plasmid is transferred to water peanuts by using a leaf disc method to increase the tolerance and accumulation of plants on Zn, Cd and Mn, and transgenic plants with high accumulation of heavy metal elements are screened and cultivated. The transgenic water peanuts can repair the wastewater polluted by metal elements such as Zn, Cd and Mn. The technology can be widely applied to the field of biological repair of heavy metal polluted soil and water bodies, and has good repair effects and broad application prospects.

The invention provides Staphylococcus caprae and application thereof. The bacterial strain is named CL, and the collection number of the bacterial strain is CCTCC NO:M2016305. By adopting the staphylococcus caprae, phenol in 200mg / L liquid culture medium of phenol (10g of MgSO4.7H2O, 0.2g of CaCl2.2H2O, 2 to 5g of KCl, 2.5g of tryptone, 10g of yeast extract, 5 percent of NaCl and distilled water are added to 1,000ml, pH is adjusted to be about 7.2, and sterilization is performed at the temperature of 121 DEGC for 15 minutes) can be degraded by 90 percent or more within 36 hours. The Staphylococcus caprae CL disclosed by the invention has a higher phenol degrading capability, can be applied to biological repair of phenol-polluted environments, and has higher economical value and a better application prospect.