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Method for preparing soil composite biological repairing agent by using maize straws and application of repairing agent in soil property improvement

A technology of bioremediation agent and corn stalks, which is applied in the restoration of polluted soil, etc., can solve the problems of destroying soil ecological balance and affecting the effect of preventing and controlling soil-borne diseases, so as to improve the micro-ecological environment, increase the quantity, and well inhibit The effect of action

Inactive Publication Date: 2012-07-18
YANGZHOU POLYTECHNIC INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It can be seen that a large number of pesticide residues and pathogenic bacteria exist, destroying the ecological balance of the soil
[0003] In addition, the presence of a large number of chemical fungicides in the soil can also kill or inhibit the growth and reproduction of the biocontrol Trichoderma T22, affecting its effect on preventing and controlling soil-borne diseases

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] The present invention is carried out according to the following process steps:

[0025] 1) Raw material processing: Choose the mold-free corn stalk and bran mixture and crush it to 30 meshes for use;

[0026] 2) Liquid culture: its medium components include 8ml of potato extract, (NH 4 ) 2 SO 4 0.4g, K 2 HPO 4 0.3g, C a CO 3 1g, add water to 100ml, adjust the pH value to 6, autoclave for 30min, after the material cools, connect to the activated Trichoderma strains T8-2 and T22, and culture at 25℃, 180 revolutions / min. Until OD is measured with a spectrophotometer 600 When the value reaches 0.35, liquid fermentation will be stopped, and the two fermentation liquids will be used as seed liquids for standby;

[0027] 3) Solid culture: Take 8g of crushed corn stalk powder, 2g of bran powder, 0.9g of glucose, (NH 4 ) 2 SO 4 0.4g, KH 2 PO 4 0.2g, MgSO 4 ·7H 2 O0.1g, water 100ml; the fermentation method is: first dissolve the above glucose and inorganic salt with water, then mix with...

Embodiment 2

[0043] The present invention is carried out according to the following process steps:

[0044] 1) Raw material processing: select the mold-free corn stalk and bran mixture and crush it to 40 meshes for use;

[0045] 2) Liquid culture: its medium components include potato extract 10ml, (NH 4 ) 2 SO 4 0.2g, K 2 HPO 4 0.2g, C a CO 3 1g, add water to 100ml, adjust the PH value to 6.5, autoclave for 20min, after the material cools, connect to the activated Trichoderma strains T8-2 and T22, and culture at 25℃, 200 revolutions / min. Until the OD is measured with a spectrophotometer 600 When the value reaches 0.30, liquid fermentation will be stopped, and the two fermentation broths will be used as seed liquids for standby;

[0046] 3) Solid culture: Take 10g of crushed corn stalk powder, 3g of bran powder, 0.9g of glucose, (NH 4 ) 2 SO 4 0.2g, KH 2 PO 4 0.1g, MgSO 4 ·7H 2 O0.08g, water 100ml; the fermentation method is: first dissolve the above glucose and inorganic salt with water, then mix...

Embodiment 3

[0062] The present invention is carried out according to the following process steps:

[0063] 1) Raw material processing: select the mold-free corn stalk and bran mixture and crush it to 35 meshes for use;

[0064] 2) Liquid culture: The medium components include 9ml of potato extract, (NH 4 ) 2 SO 4 0.3g, K 2 HPO 4 0.25g, C a CO 3 1g, add water to 100ml, adjust the PH value to 6.2, autoclave for 25min, after the material cools, connect to the activated Trichoderma strains T8-2 and T22, and shake at 25-28℃, 190 revolutions / min Incubate until OD is measured with a spectrophotometer 600 When the value reaches 0.32, liquid fermentation will be stopped, and the two fermentation broths will be used as seed liquids for standby;

[0065] 3) Solid culture: Take 9g of crushed corn stalk powder, 2.5g of bran powder, 0.85g of glucose, (NH 4 ) 2 SO 4 0.3g, KH 2 PO 4 0.15g, MgSO 4 ·7H 2 O0.09g, water 100ml; the fermentation method is: first dissolve the above glucose and inorganic salt with wate...

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Abstract

The invention relates to a method for preparing a soil composite biological repairing agent by using maize straws and the application of the repairing agent in soil property improvement. According to the method, maize straws which are low in cost and renewable are adopted as raw materials, treated trichodermahamatums T22 and T8-2 are taken as strains, and then liquid-liquid two-phase mixed strainfermentation technology is carried out to prepare the soil composite biological repairing agent. The repairing agent has a good repairing effect on soil, a good degradation effect on pesticide residues in soil, a strong restraining or killing effect on soil spreading pathogenic bacteria and a promoting effect on crop growth and is harmless to ecological environment.

Description

Technical field [0001] The invention relates to a preparation method for preparing a soil composite bioremediation agent from corn stalks and its application in improving soil quality, belonging to the technical field of biological preparation methods and degradation. Background technique [0002] Due to the non-easy migration of facilities in greenhouse cultivation, crops are often replanted in the greenhouse, and soil-borne pathogens in the soil multiply and multiply, causing plant diseases. The frequent occurrence of diseases has prompted farmers to use pesticides more frequently, leading to increased pesticide residues in the soil, forming a vicious circle. Taking greenhouse vegetables as an example, we found through the biological detection of the soil inside and outside the shed: the residue of carbendazim in the soil in the shed was as high as 9.6 mg / kg dry soil, which was 6.7 times that of the outside of the shed, and the number of pathogenic colonies reached 3.9 ×10 5 P...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B09C1/10
Inventor 田连生曹雨平张新科秦建华王武林陈菲
Owner YANGZHOU POLYTECHNIC INST
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