9results about How to "Improve micro-ecological environment" patented technology

The invention relates to a trichoderma asperellum and pseudomonas aeruginosa compound microbial agent as well as a preparation method and application thereof. The compound microbial agent is prepared from a pseudomonas aeruginosa solid fermentation product, a trichoderma asperellum solid fermentation product, an auxiliary agent and a synergist, the number of effective spores in the compound microbial agent is greater than 10 < 10 > CFU / g; the pseudomonas aeruginosa is preserved in the China General Microbiological Culture Collection Center (CGMCC) on September 13, 2022, and the preservation number of the pseudomonas aeruginosa is CGMCC No.25697; the trichoderma asperellum is preserved in the China General Microbiological Culture Collection Center (CGMCC) on August 9, 2023, and the preservation number of the trichoderma asperellum is CGMCC No. 40752. The compound microbial agent can effectively solve the problems of soil-borne diseases such as pepper phytophthora blight and continuous cropping obstacles in the prior art, and has an obvious growth promoting effect on melon and vegetable plants such as pepper.

This invention belongs to the field of *Coptis chinensis* cultivation technology, specifically relating to a cultivation method for increasing the berberine content in *Coptis chinensis*, comprising the following steps: seedling cultivation, transplanting, microbial inoculation, light management, fertilization management, staged drought stress induction, water management, and harvesting; the microbial inoculation involves simultaneously applying a compound microbial agent containing *Bacillus amyloliquefaciens*, arbuscular mycorrhizal fungi, and plant rhizosphere growth-promoting bacteria at the time of transplanting; the light management involves shading combined with LED supplemental lighting; the staged drought stress induction involves controlling the relative soil moisture content to 45%-55% for 7-10 days after transplanting, 60-75 days after transplanting. This invention, through the synergistic effect of microbial inoculation, light quality regulation, and drought stress induction, can significantly increase the berberine content in the roots of *Coptis chinensis* by more than 120% compared to conventional cultivation methods, and is standardized, green, and sustainable, suitable for large-scale cultivation and promotion.

The invention discloses compound lactic acid bacteria, a metagen and a preparation method and application of the compound lactic acid bacteria and the metagen. Through composite compatibility of the probiotics separated from the yak yoghurt in the Qinghai-Tibet Plateau, a synergistic effect of various strains is exerted, negative effects caused by treatment by using a medicine means are avoided, side effects are avoided, and the problems of single effective microbial community, low regulation capability and the like in the prior art are solved. By adding the selective auxiliary materials, the survival rate of the compound probiotics after freeze-drying treatment is improved, the probiotics can more effectively resist the adverse environment of the gastrointestinal tract, the problems that in the prior art, the probiotics are prone to being damaged by gastric acid, the survival rate is reduced in the storage process, and a large amount of probiotics are inactivated before entering the intestinal tract are solved, the planting rate of the probiotics is increased, and the survival rate of the probiotics is increased. The effect is more durable and more stable. Through synergistic compatibility of the composite probiotics and functional prebiotics from characteristic plant resources from the Tibet Plateau, the intestinal flora balance adjusting capacity is improved, and the beneficial effects of effectively improving symptoms such as constipation and obesity are further achieved.

The preparation method of the modified water chestnut shell biochar-based organic slow-release fertilizer comprises the following steps: S1, preparing modified biochar, taking a proper amount of water chestnut shells, washing with distilled water, drying, screening with a mesh screen, adding a potassium ferrate-potassium oxalate composite activator, fully stirring in pure water for 30-60 minutes, and drying in a drying box to obtain the modified biochar; grinding and sieving with a 100-mesh sieve to obtain modified charcoal; s2, preparing a fermented organic fertilizer, namely selecting a manure fermentation inoculant, adding dry cow dung and a microbial inoculant in proportion, then adding a proper amount of water to obtain a mixture, and continuously fermenting the mixture at a certain temperature for a period of time to obtain the fermented organic fertilizer; s3, preparing the biochar-based slow-release fertilizer, namely mixing the modified biochar, the fermented organic fertilizer and montmorillonite, adding the mixture into a disc granulator, granulating, slowly adding tap water into the disc granulator, stopping adding the tap water after obvious particles appear, and running the granulator for a period of time to obtain the granular biochar-based slow-release fertilizer.

The invention discloses a three-dimensional composite interplanting method for interplanting poria cocos and peanuts. The three-dimensional composite interplanting method comprises the following steps: S1, soil preparation; s2, applying a composite soil conditioner and building furrows; s3, putting the poria cocos into a cellar; s4, peanut sowing; s5, field management in the symbiotic period; s6, harvesting in a staggered manner. According to the method, the poria cocos is planted underground, the peanuts are planted on the ground, after the peanuts are harvested, the ground surface is covered with peanut straw, the poria cocos is shaded, the physical properties of soil are improved by returning the peanut straw to the field, two kinds of medicines and grains are planted in one field through poria cocos and peanut interplanting, and the land utilization rate is increased; meanwhile, the composite soil conditioner prepared by a specific method is applied, long-acting and synergistic biological stimulation is provided for the two crops, the soil permeability is improved, and special pretreatment is performed on pine sections, so that the poria cocos inoculation rate, stress resistance and yield of poria cocos strains are remarkably improved, the soil micro-ecological environment is improved, the soil permeability and enzyme activity are improved, and the yield of the poria cocos strains is increased. And double improvement of economic benefits and ecological benefits is realized.

This invention provides a cultivation substrate, its preparation method, and its application, belonging to the field of tailings utilization technology. The cultivation substrate, by weight, comprises: 1-7 parts of an algae-bacterial symbiosis, 1-12 parts of modified illite tailings, and 1-22 parts of sheep manure; the algae-bacterial symbiosis includes *Chlorella vulgaris* and *Streptomyces flavus*. The cultivation substrate provided by this invention meets the requirements of standard NY / 2118-2012 for vegetable seedling substrates, improving soil physicochemical properties while also promoting plant growth and enhancing plant stress resistance. The cultivation substrate provided by this invention has advantages such as environmental friendliness and sustainable utilization, aligning with the development trend of modern agriculture, and providing a new direction for the resource utilization of illite tailings; it also achieves efficient recycling of mine tailings and agricultural waste, providing an innovative path for green agricultural development and resource utilization.

The application belongs to the technical field of microorganisms, and discloses a salt and alkali tolerant squirrel mammalian coccus and application thereof. Mammaliicoccus sciuri ​ STM10, which is a gram-positive coccus, has been preserved in the China General Microbiological Culture Collection Center with a preservation number of CGMCC No.38450. The strain has salt and alkali tolerance, can play the promoting functions of nitrogen fixation, phosphorus dissolution and resolution, iron carrier production and protease production, and can be prepared into microbial fertilizer. Application of the microbial fertilizer can effectively relieve salt and alkali stress, improve the seed germination and growth ability of crops, improve the physical and chemical properties of saline-alkali soil, optimize the ion environment, promote the nutrient absorption of crops, and provide support for agricultural utilization of saline-alkali land.

The invention discloses a processing method of a plant-based beverage capable of promoting digestion and absorption. The processing method comprises the following specific steps: step 1, selecting raw materials; 2, pretreating the raw materials; step 3, grinding and extracting; step 4, microbial fermentation; after fermentation is completed, filtering and clarifying are carried out, and impurities and microbial thalli in fermentation liquor are removed; step five, blending and homogenizing; and step 6, sterilizing, and then naturally cooling to obtain a beverage finished product. And step 7, packaging. According to the processing method of the plant-based beverage capable of promoting digestion and absorption, fresh vegetables, fruits, beans and other food are used as raw materials, and a scientific formula and a processing method are adopted to prepare the plant-based beverage. The prepared plant-based beverage contains rich plant nutrients, contains rich vitamins, proteins, amino acids, minerals and other nutritional ingredients, is beneficial to body health, and can regulate the flora balance in intestinal tracts, so that the intestinal micro-ecological environment is improved, and digestion and absorption are facilitated.

This invention belongs to the field of microbial technology, and relates to a probiotic agent containing *Lactobacillus rhamnosus* CL-03 for relieving eczema, its preparation method, and its application. The strain in the agent is *Bifidobacterium longum* subsp. *infantile* with accession number GDMCC No:64541. Bifidobacterium longum subsp.infantis Strain C-3 and Lactobacillus rhamnosus with accession number GDMCC No. 65725 Lactobacillus rhamnosus Composition of CL-03 strain. The probiotic agent prepared by this invention can relieve eczema, repair the skin barrier, and inhibit the proliferation of harmful bacteria on the skin. Moreover, the Bifidobacterium longum subsp. infantis C-3 strain and Lactobacillus rhamnosus CL-03 strain have a certain synergistic effect on the above-mentioned effects.