681 results about "Plant hormone" patented technology

The subject invention concerns materials and methods for controlling agricultural traits in plants that are mediated by the plant hormone ethylene. One aspect of the invention concerns a polynucleotide that comprises a sequence encoding a mutant ethylene receptor that is operably linked to a regulatory sequence that drives expression of the mutant receptor in a tissue-specific manner. In an exemplified embodiment, the mutant receptor sequence is an etr1-1 sequence, or a functional fragment or variant thereof, and the regulatory sequence is a promoter sequence from a cotton chitinase gene that can promote expression of the mutant ethylene receptor in abscission zone tissue of a plant. The subject invention also concerns plants and plant tissue transformed with the polynucleotide of the subject invention. Plants expressing the polynucleotide of the subject invention do not drop their flowers in response to exposure to ethylene.

A liquid fertilizer containing wood vinegar liquid or oxalic vinegar liquid and its production are disclosed. The liquid fertilizer consists of urea 1í½20.0%, potassium phosphate 1í½20.0%, potassium nitrate 1í½20.0%, lime nitrate 0.1í½10.0%, magnesium sulfate 0.1í½5.0%, ferrous sulfate 0.1í½5.0%, manganous sulfate 0.1í½5.0%, zinc sulfate 0.1í½5.0%, cupric sulfate 0.1í½5.0%, boron sand or boric acid 0.1í½2.0%, sodium molybdate or ammonium molybdate 0.1í½1.0%, plant hormone or plant regulator, rare earth element or humus acid or pesticide auxiliaries etc. The process is carried out by proportioning and mixing. It can improve bacterium inhibiting and plant growth regulating functions.

The invention discloses a preparing method of alkaline soil modifier, which comprises the following steps: dissolving polyacrylic acid in the water; adding inorganic acid, pyroligneous and plant hormone sequently; stirring to blend evenly. The modifier contains 5-25% polyacrylic acid, 10-50% inorganic acid, 1-5% pyroligneous, 0.01-2.0% plant hormone and water. The invention reduces soil pH value effectively, which is beneficial for plant growth.

The invention belongs to the technical field of agriculture and environmental pollution improvement and relates to a heavy metal resistant plant endogenous nodule bacterium and application thereof. The heavy metal resistant nodule bacterium is preserved at the China center for type culture collection (CCTCC) with the preservation date to be 2012/9/18 and culture preservation number to be CCTCC NO:M2012357. The bacterial strain liquid preparation contains more than a billion of effective living bacteria per milliliter, and the bacterial strain solid preparation contains 0.2 billion of effective living bacteria per gram. The heavy metal resistant nodule bacterium strain can secrete plant hormones indole acetic acid (IAA) and siderophores, and resists heavy metal copper and cadmium. When plants are planted in moist soil or tailings containing heavy metal and bioremediation preparation is inoculated, the heavy metal resistant nodule bacterium can promote plant growth and absorbs heavy metal copper, can improve plant restoration efficiency, can fix sandy soil and reduces water and soil loss.

The invention relates to the field of biotechnology and discloses an agilawood production method. The method comprises the steps of first drilling a hole in a trunk of aquilaria malaccensis or aquilaria sinensis, enabling an agilawood inducer formed by plant hormones, inorganic salt and phenethyl alcohol to be added dropwise into the tree in a transfusion mode, then sealing the hole, adding the agilawood inducer dropwise at an interval of 1-2 months, performing treatment for 3-24 months in total, then cutting brown oily matter and yellowish-brown discolored xylem in the trunk, and obtaining agilawood after drying in the sun. The plant hormones are one or more than two of methyl jasmonate, ethephon and 6-BA; and the inorganic salt is one or both of sodium chloride and magnesium chloride. Components of the agilawood inducer are adjusted on the basis of an existing agilawood inducer, a trunk defense system is excited, related enzymes for synthesizing agilawood chemical components are generated and act on phenethyl alcohol, the trunk generates related chemical components in the agilawood, and finally the agilawood is formed by accumulation. According to the method, not only are contents of agilawood oil and sesquiterpenes in the agilawood oil improved, but also contents of alcohol-soluble extracts are improved.

A composition for enhancing resistance to plant diseases comprising (1) chitosan, (2) lactic acid or succinic acid or both of the two, or the two and glutamic acid, and as the essential or optional ingredient (3) a plant activating agent such as a plant hormone, etc. It can protect plants without badly affecting the environment.

The invention discloses an emmenopterys henryi pollution-free planting method which comprises the steps of selection of seeds, disinfection of the seeds, centrifugal overweight treatment, plant hormones and low temperature sand stratification treatment, seed germination and soil scientific management, seedling transplanting and the like. By the centrifugal overweight treatment and the plant hormones and low temperature sand stratification treatment, sleep time of the seeds of emmenopterys henryi is shortened; moreover, by effective temperature and light treatment, the emmenopterys henryi has high seed germination rate, germinability and germination index.

The invention provides a method for inducing fresh water chlorella to fast accumulate grease through plant hormone jasmonic acid. The method is characterized by comprising the following steps of: (1) preparation of chlorella solution preparation: under the conditions that the temperature is 22-24 DEG C, the light intensity is 1800-2000lx, and the light-to-dark ratio is 12h/12h, culturing fresh water chlorella to logarithmic phase to obtain chlorella solution for conducting hormone induction, wherein the fresh water chlorella adopts NO.9 fresh water chlorella or NO.31 fresh water chlorella which both come from Freshwater Algae Culture Collection at the Institute of Hydrobiology, FACHB-collection; (2) acquisition of high-grease-content chlorella: adding jasmonic acid into 200ml of chlorella solution obtained in step (1) until the final concentration is 0.49-0.51mg/l; and (3) acquisition of high-grease-content chlorella: culturing the chlorella solution subjected to hormone induction in step (2) for 15 days under the conditions that the temperature is 22-24 DEG C, the light intensity is 1800-2000lx, and the light-to-dark ratio of 12h/12h, and centrifugally collecting the frond with high grease content. The method is simple and easy, low in cost, and the grease content of the target product is high, and can be used as a raw material of industrialized biological produced oil.

The invention discloses a tomato S1lPIF4 gene, protein and application thereof to improving the low temperature resistance of plant. The nucleotide sequence of the S1lPIF4 gene is SEQ ID NO.1, and thecorresponding amino acid sequence is as shown in SEQ ID NO. 2. A tomato SlIPIF4 overexpression plant or a gene knockout plant through the gene means to regulate the expression level of the S1lPIF4 gene in order to do research on the regulatory mechanism of the S1lPIF4 gene on the low temperature resistance of tomato. According to the result, overexpression of S1lPIF4 at the lower temperature canpromote accumulation of plant hormone abscisic acid (ABA) and jasmonic acid (JA), inhibits accumulation of gibberellin (GA), further induces expression of the low temperature resistant gene of the tomato, and finally improves the low temperature resistance of the tomato. Therefore, the S1lPIF4 gene of the tomato enhances the low temperature resistance by inducing formation of hormones ABA and JA in the body. The tomato S1lPIF4 gene provides the gene resource for breeding new varieties of low temperature resistant tomato, has the good potential application value, and lays theoretical basis fordoing research on tomato plant distress response signal mechanism and molecular mechanism of being tolerant to adverse environment.

The present invention relates to a novel culture system for generating transformed corn plants from mature seeds. In particular, the invention relates to the use of plant hormones during germination to affect the culture response. Transgenic corn plants can then be easily produced.

The present invention has incorporated a non-lethal negative selectable marker gene into the vector backbone DNA of a DNA plasmid used to transform plant cells. These transgenes are designed to express a non-lethal gene product in plant cells that contain the vector backbone DNA of the DNA plasmid. The gene products of the non-lethal negative selectable marker gene are involved in plant hormone biosynthesis pathways, plant hormone substrate diversion, plant hormone degradation, plant hormone signaling or metabolic interference. The use of these DNA plasmids to transform plant cells provides for the enhanced production of commercially viable plants.

The invention relates to hormone-free tissue culture and a rapid propagation method of anoectochilus formosanus seedlings. The method comprises the following four tissue culture stages: 1) seeds germin ate without bacteria to form protocorms; 2) the protocorms is propagated and differentiation culture is performed on plantlets; 3) strong seedlings of aseptic seedlings are cultured; 4) rooting culture is performed, and different hormone-free culture media are adopted at different tissue culture stages. The method provided by the invention has the advantages that the operation is simple and convenient, the process is less, the cost is low, frequent subculture is not required to be performed on tissue culture seedlings, exogenous plant hormones are not used during the whole culture process, the problems that a large number of exogenous plant hormones are used, the production cost is high, the seedlings are irregular, and the seedlings are unsafe to eat exist in the conventional process of culturing anoectochilus formosanus tissues are solved better, the quality and the transplanting survival rate of the seedlings are improved, and security of using the seedlings is improved.

The invention belongs to the field of microalgae biotechnologies, and particularly relates to a method for synthesizing astaxanthin by inducing chlorella vulgaris by using plant hormones and iron ions. The method specifically comprises the steps of: activating and culturing the chlorella vulgaris in a liquid culture medium to obtain a synchronously growing chlorella vulgaris solution for later use; and taking the chlorella vulgaris solution growing to an exponential growth phase, adding the plant hormones and the iron ions in the chlorella vulgaris solution, replenishing a carbon source and a nitrogen source for culturing, ending the culturing until the accumulation of the astaxanthin in chlorella vulgaris cells is maximum (the accumulation of the astaxanthin is determined by adopting a liquid chromatography during sampling), and harvesting cell disruption and extracting the astaxanthin. The method is simple and easy to operate and low in cost, and is capable of remarkably increasing the yield of the astaxanthin, thereby greatly increasing the efficiency of producing the astaxanthin by the chlorella vulgaris.

The invention provides a novel abscisic acid type synthetic plant hormone benzoiso abscisic acid (II) which has good abscisic acid type plant growth adjustment activity in a raceme form or in a single optical living form, wherein the activity of the R-body (compound II-a) is superior to the natural abscisic acid. The novel abscisic acid type synthetic plant hormone benzoiso abscisic acid can be used in the agriculture production as the abscisic acid type synthetic plant hormone.

Methods for treating a plant comprise contacting a plant or a part of a plant with one or more of 9-oxononanoic acid, arachidonic acid, or a salt or ester thereof; wherein the amount is effective to increase tolerance to abiotic stress in the plant or to reduce a consequence of abiotic stress in the plant. Additional ingredients can be included such as dicarboxylic acids, pipecolic acid, or salicylic acid, or salts/esters thereof; sunblocks such as kaolin or calcium carbonate; carriers such as inert powders or liquids; and co-treatment materials such as fertilizers, plant nutrients, biostimulants, micronutrients, amino acids, plant hormones, pesticides, fungicides, insecticides, nematicide, stearic acid, vegetable oil, or phospholipid.

The invention discloses a method for preparing organic seaweed liquid fertilizer by fermentation and enzymolysis of microorganisms and belongs to the field of fertilizer production. Seaweeds are degraded through a compound enzymolysis technology and a microorganism fermentation technology; a complicated enzyme system produced through microorganism fermentation and a compound enzyme including proteinase, cellulase, pectinase, polysaccharide degradation enzyme and the like are used for degrading the seaweeds step by step; seaweed cell walls are removed so that contents including alginic acid, polysaccharide, plant hormones and the like are sufficiently released; the decomposition of active substances including the alginic acid, plant growth promotion factors and the like is avoided under moderate technological condition; effective active components in the seaweeds are kept to the greatest extent. According to the method disclosed by the invention, energy saving, water saving, low carbonand environment protection are realized; the seaweed biological organic liquid fertilizer contains rich mineral substances and effective components including macro-elements, microelements and the likeneeded by the growth of plants; a soil structure can be improved to keep moisture and soil and the growth of root microorganisms of the plants is promoted; the yield of applied crops is remarkably improved and the fertilizer has a great market potential.

The invention relates to a method for culturing submerged plant seeds. The method comprises the following steps: A, soaking the submerged plant seeds produced by apomixia in 0.01-1wt% of dimethoate or sodium hypochlorite solution for 1-6 hours, washing the soaked submerged plant seeds with clear water, naturally drying the soaked submerged plant seeds in the air; B, standing the submerged plant seeds at 20-25 DEG C for 1-3 days, storing at 1-5 DEG C for 1-2 days, and preserving at 30-40 DEG C for 12-36 hours; C, soaking the submerged plant seeds in solution containing plant hormone, trace elements and inorganic salt for 12-24 hours; and D, burying the submerged plant seeds in a matrix by a depth of 3-5cm, and then putting the matrix in which the submerged plant seeds are buried at the bottom of river or lake water. The matrix comprises humus, sediment at the bottom of river and lake, bank soil, clay and sand. The submerged plant seeds bred by the method have high germination rate and environmental adaptability.

The invention discloses a cherry grafting method, and belongs to the technical field of agricultural planting. The method comprises the steps of stock preprocessing, scion preprocessing, grafting, later grated seedling managing and the like. Before cherry grafting, the stock is fertilized so that the nutrient supplying of the stock can be ensured; the scion is placed in the plant hormone to be immersed so that the wound healing after grafting can be promoted; during grafting, the incision attached position of the scion and the stock is coated with organic fertilizer and the like, and then enough nutrient can be supplied to the grafted scion, the incision of the scion and the stock can be fast healed as soon as possible, and therefore the grafting survival rate is improved and reaches to more than 97 percent.

The invention relates to the technical field of agricultural planting, and discloses a grafting method for Prunus salicina. A peach tree is selected as a stock, fertilization treatment is carried out on the stock before grafting is carried out, the top tip of the stock is cut off, and the cut part is wrapped by plastic; a scion which is cut off is first soaked in plant hormones; after grafting is carried out, the position of attachment between the scion and a stock cut is coated with organic fertilizer, and then a thin-film strip is used for binding to carry out fixation with a bud is exposed; after grafting is carried out for 10 days to 15 days, the thin-film strip is released. According to the grafting method for Prunus salicina, through the treatment method that first, the stock is fertilized, then, the scion is soaked in the plant hormones, and the position of attachment between the scion and the stock cut is coated with organic fertilizer, it is ensured that the scion can have enough nutrition supply after grafting is carried out, and the survival rate of grafting is improved to be more than 97%.

An in vitro culture method for long-grained non-glutinous rice includes such steps as screening a secondary culture medium and a differential culture medium of colli, and in vitro culturing. Said culture media contain macro elements, trace elements, additives, carbon source and plant hormones.

The invention provides a pretreatment method and a simultaneous quantitative detecting method for multiple endogenous plant hormones in a plant sample. The method comprises 1) quantitatively adding isotope internal labels for the multiple endogenous plant hormones inside the plant sample, and then obtaining a sample solution through solvent extraction; 2) decontaminating the sample solution obtained from the step 1) with graphitized carbon black materials as purification agent to obtain to-be-detected solutions of the multiple endogenous plant hormones in the plant sample; 3) collecting response data of the obtained to-be-detected solutions of the multiple endogenous plant hormones in the plant sample through analytical instruments, and then simultaneously and quantitatively detecting the multiple endogenous plant hormones in the plant sample through the internal labeling method. The pretreatment method and the simultaneous quantitative detecting method for the multiple endogenous plant hormones in the plant sample are simple, rapid, solvent-economic, easy to operate, high in accuracy and capable of achieving enrichment and purification of low-content endogenous plant hormones as well as simultaneous quantitative detection.

The invention relates to a composite growth regulator for beet stress-resistant yield and sugar-increased seedling stage and a using method thereof. The composite growth regulator is prepared from plant hormone, Ca2 and various signal substances, is particularly suitable for saline and arid environment conditions, and can induce the expression of beet stress-resistant genes and accelerate coordination and unification between the beet and the environment, the individual and population, the root and the overground part, the external form of the plant and internal physiological function. After the beet is transplanted, the salinization resistance, drought resistance and disease resistance can be effectively enhanced; and the problem that the beet yield and the sugar content resulted from the influence of salinization, drought and disease spreading are reduced year by year is solved. The regulator also has the characteristics of a common strong-seedling agent and also can solve the problems of vain growth, disease susceptibility and weak seedlings of the young seedlings in the beet seeding culture process; the beet root yield under the saline and arid environment conditions reaches 47,267.47kg/hm<2>, the yield is increased by 16 to 18 percent; the sugar content is 16.1 percent, and is increased by 0.9 to 1.2 percent; and the effects of increasing the yield and sugar are obvious.

The invention relates to a high-efficiency fruit-nutritive physiological hyperchromic fertilizer and a preparation method thereof. Currently, the hyperchromic products in the market such as phytohormone, including products of ethane, abscisic acid, daminozide, most of such products adopt or contain hormone, have instable effect and easily cause adverse side effects, thus leading fruits to be soft and lowered in bin stability. The invention is a high-efficiency fruit-nutritive physiological hyperchromic fertilizer, the active ingredients of which comprise aminofusin, potassium sulfate, potassium chloride, borax, zinc vitriol, iron vitriol (FeSO4 question mark 7H2O), compound sodium nitrophenolate, chlormequat chloride with 50 percent of aqua and white sugar; the ingredients do not contain hormone, are non-poisonous and pollution-free, and have no adverse side effects. The high-efficiency fruit-nutritive physiological hyperchromic fertilizer contains various aminophenols, active substances for plant growth, a plurality of major elements and chelate trace elements, which are necessary for the growth and development of the fruit trees and vegetables, and is a novel organic and inorganic hyperchromic product integrating the advantages of various foliar fertilizers of inorganic nutrition type, organic type and growth conditioner type, etc. into a whole.

The invention discloses a method for measuring the content of endogenous hormones of burgeons of a tea tree on the basis of high performance liquid chromatography (HPLC). Conditions such as composition, concentration and flow speed of a flowing phase and the adding proportion and the detection wavelength of organic modifying agents are optimized, an optimal chromatographic condition of the content, which is measured by the HPLC, of the endogenous hormones of the burgeons of the tea tree is determined, and repeatability and precision of the method are inspected. The method has characteristics of high speed, high efficiency, high sensitivity and high automation; a tea tree terminal bud sample preparation method and selection and chromatographic conditions of the flowing phase are explored when the hormones of terminal buds of the tea tree are measured by the high performance liquid chromatography, and the method for measuring plant hormones by using the HPLC successfully is reliable and speedy.

The present invention is related to a formulation based on micronized natural calcite mineral consisting of: A. mineral part which is characterized: (i) by the particle size with diameter smaller than 5 μm, where the distribution of particle size is such that minimum of 10% of particles are with diameter under 900 nm, with total surface of particles greater than 3.2 m²/g, and with total porous volume greater than 0.012 cm³/g, and by composition: (ii) CaCO₃, MgCO₃, FeCO₃, MnCO₃, ZnCO₃ and SiO₂ in mixtures where CaCO₃ is presented in amount from 10% to 99% of mineral part; and B. adjuvant selected from the group consisting of: wetting agent, drift-control agent at application by spraying, humic acid salt, amino-acids salts, complex of plant micro-nutrients, vitamin, plant hormone, nitrogen fertilizer, potassium salt, borate salt, molybdate salt, plant extract, chlorophyll, yeast extract, or mixture of these substances; where A. and B. form an aqueous suspension wherein percentage of A. and B. is lower than 5% by weight. The formulation from this invention is used as a plant booster and mineral fertilizer for stimulation of photosynthetic process in plants, stimulation of plants growth, reinforcement of plant resistance to physiological and anhydrous stress, reinforcement of resistance to different plant diseases, for improvement of nutrient absorption, for increasing crop yields, and for plant nutrition.