78 results about "Plant hormone" patented technology

The invention discloses a method for detecting natural plant hormones and exogenous growth regulators of vegetables, efficient separation and accurate detection of the natural plant hormones and the exogenous growth regulators are realized through combination of hydrophilic interaction chromatography and mass spectrometry, a hydrophilic interaction chromatographic column is adopted, and conditions such as mobile phase composition and flow velocity are optimized; the selectivity of polar substances is improved by utilizing hydrophilic interaction, so that compounds with similar structures achieve baseline separation, and chromatographic peak overlapping interference is avoided; the mass spectrometric detection only responds to a specific ion signal by screening characteristic parent ion / daughter ion pairs of a target object, so that matrix impurities and non-target ion interference are effectively eliminated, the false positive risk is remarkably reduced, and the detection sensitivity and specificity are improved; the combined technology is combined with the efficient separation capacity of the hydrophilic interaction chromatographic column and a multi-reaction monitoring mode, synchronous separation of multiple compounds with similar polarities in single analysis is achieved, parallel detection of dozens of target objects is supported, and the analysis time is greatly shortened.

The invention discloses a thick-stem high-yield caulis spatholobi germplasm screening and breeding method suitable for an under-forest habitat. According to the method, a standardized under-forest cultivation system is constructed, spiral winding induction synergistically mediated by plant morphology and plant hormones is implemented, lignification development of stems is enhanced by applying mild mechanical stress with specific parameters, rhizosphere microorganism regulation and systematic nutrition management are synchronously combined, and the yield of the stems is increased. And finally, a multi-dimensional comprehensive evaluation system covering the stem shape, the wood density, the active ingredient content and the under-forest weak light adaptability is established, so that an integrated technical scheme for efficiently and accurately breeding the excellent caulis spatholobi germplasm with thick stem phenotype, high flavone accumulation and strong under-forest weak light tolerance from a large number of candidate germplasm is realized.

The invention discloses a method for preparing a soil conditioner for gardenia cultivation by using immobilized compound bacteria, which comprises the following steps: extracting gardenia lignin by using gardenia waste as a raw material, and preparing an inner-layer wall material to embed a mixed bacteria solution to obtain lignin inner-layer microcapsules; preparing an outer-layer wall material through modified chitosan, and compounding and curing the inner-layer microcapsule, the hydrogenated streptomyces liquid and the outer-layer wall material to obtain the immobilized composite microbial agent. And finally, mixing the immobilized complex microbial inoculant with matrixes such as leaf mold and peat soil to obtain the modifier. Ordered release of compound bacteria is realized through dual response, functional synergy of Rafissonia, Ochrobactrum and Streptomyces hydrogenii can be enhanced through population signal molecule modification, soil nutrients can be activated, effective iron can be chelated to solve the problem of iron deficiency yellowing of cape jasmine, soil pollutants and pesticide residues are degraded, and the soil quality is improved. Pathogenic bacteria reproduction is inhibited, and rhizosphere micro-ecological balance is maintained; plant hormones can be secreted to promote the growth of the cape jasmine, and the cape jasmine is converted into more components with high medicinal value.

The invention relates to the technical field of agricultural cultivation, in particular to a breeding method of water horn seeds. The method comprises the following steps: storing dried water chestnut seeds at 8-16 DEG C for 8-24 days, and soaking the water chestnut seeds in a potassium permanganate aqueous solution for 15-25 minutes; and sowing the soaked water horns seeds in a matrix, and culturing for 18-22 days to obtain seed seedlings. According to the method provided by the invention, the germination rate of the water pod seeds is extremely high and reaches 99%; and the method is short in operation period and simple in operation steps, does not need any plant hormones and chemical substances, effectively solves the breeding problem of the water hormones, and has important significance for reducing the endangered degree of the water hormones.

The invention relates to the technical field of sugarcane smut resistance, and provides a molecular marking method for sugarcane smut resistance, which comprises a sample preparation unit, a double-sugarcane area inoculation identification unit, a whole genome association analysis unit, an SNP (Single Nucleotide Polymorphism) marker development unit, a marker verification unit and a result application unit, the output end of the sample preparation unit is connected with the input end of the double-sugarcane-area inoculation identification unit through a sample transmission channel, the double-sugarcane-area inoculation identification unit outputs phenotypic data to the whole-genome association analysis unit, and the whole-genome association analysis unit outputs remarkably associated SNP sites to the SNP marker development unit; and the SNP marker development unit outputs a specific primer to the marker verification unit. The molecular marker provided by the invention is based on the correlation between the candidate gene of the site and the known disease-resistant pathway of the plant, and the MYB transcription factor can improve or reduce the resistance level of the plant to pathogenic bacteria by regulating the synthesis of secondary metabolites, transduction of plant hormone signals, expression of defensive genes and the like.

The present application belongs to the technical field of microorganisms, and relates to a Pseudomonas jessenii JY2025 and application thereof. Pseudomonas piscicola The biological preservation number of the Pseudomonas jessenii (P. jessenii) JY2025 is CGMCC No. 37283. The strain provided by the present application has broad-spectrum antifungal activity, can significantly inhibit the growth of various plant pathogenic fungi, and has the abilities of salt and alkali tolerance, inorganic phosphorus degradation, protease secretion, siderophore secretion and indole acetic acid production, which are helpful to promote crop growth and improve soil fertility.

The invention relates to the field of agricultural biochemical engineering and plant protection, and particularly discloses a liquid film-forming agent for preventing crops from freezing injury due to late spring coldness and a preparation method of the liquid film-forming agent. The film-forming agent is prepared from carboxymethyl chitosan, sodium carboxymethyl cellulose, kasugamycin, brassinolide, glycol and glycerol mixed dihydric alcohol, Tween 80 and deionized water according to a specific proportion. The preparation method comprises the key processes of step-by-step dissolution, mixing, functional component addition and the like. Organic fusion of physical barrier, ice nucleus inactivation and physiological regulation is realized through multiple action mechanisms of natural polymer composite film forming, biopesticide targeted sterilization, plant hormone induced cold resistance and dihydric alcohol synergistic antifreezing. The product can provide efficient protection at a low temperature of-4 DEG C, significantly reduces the frost damage rate, has the characteristic of 28-day natural rapid degradation, and solves the technical bottlenecks of single function, difficult degradation and poor environmental compatibility of a traditional film agent.

To provide a reagent and a methyl salicylate sensor for detecting methyl salicylate which is a plant hormone released when a plant is infected with a pathogenic fungus in the cultivation of the plant including a field crop, to provide a method for sensing methyl salicylate using the same, and to provide a method for detecting the pathogenic fungus infection of the plant.SOLUTION: A reagent for detecting methyl salicylate, comprising a terbium compound and an amine. A methyl salicylate sensor includes a methyl salicylate capture part having the reagent, and a detection part for detecting that methyl salicylate is captured by the capture part.SELECTED DRAWING: None

The invention relates to a plant cultivation method, in particular to a sterile miniature cuttage rapid propagation method for brassica napobrassica. According to the method, stem segments with buds or terminal buds of the brassica napobrassica are used as explants for asexual propagation, budlet micro cutting is performed in a sterile environment, plantlets with roots are directly induced without adding any plant hormones and inducing cluster buds and calluses, and rapid propagation of the brassica napobrassica is realized through continuous micro cutting. The method is not limited by seasons and propagation materials, seedlings can be produced in a manual control environment all the year round, the propagation speed is high, and the coefficient is large.

This invention discloses a culture medium for Antrodia camphorata mycelium, which consists of two parts: a solid medium and a liquid medium. The liquid medium contains glucose, peptone, anhydrous magnesium sulfate, and plant hormones; the plant hormones include one or more of indolebutyric acid, lignin, gibberellin, 6-benzylaminopurine, and 24-epibrassinolide. The solid medium contains carrot powder, potato powder, glucose, and agar powder; the solid medium also contains plant hormones, including one or more of indolebutyric acid, lignin, gibberellin, 6-benzylaminopurine, and 24-epibrassinolide. During cultivation, activated mycelium is first inoculated onto the solid medium to form high-quality colonies, and then inoculated onto the liquid medium for further expansion. This method significantly increases mycelium yield and the production of crude polysaccharides and crude triterpenes, providing strong technical support for the development and utilization of Antrodia camphorata.

The invention belongs to the technical field of environmental restoration, and particularly relates to cyclodextrin cross-linked supramolecular hydrogel as well as a preparation method and application thereof. A construction unit of the supramolecular hydrogel comprises double-bond modified beta-cyclodextrin and acrylic acid interacting with the double-bond modified beta-cyclodextrin through a covalent bond; plant hormones are included in a cavity of the double-bond modified beta-cyclodextrin. The supramolecular hydrogel adhesive tape is provided with charges and cavities, can include plant hormones, and can be used together with soil improvement plants such as green manure to enhance the saline-alkali soil improvement effect by regulating and controlling the salinity of plant rhizosphere soil, improving the emergence rate, promoting plant growth and improving the environmental stress resistance of the plants. Cavities of the double-bond modified beta-cyclodextrin can effectively include plant hormones, and the supramolecular hydrogel is formed through covalent interaction between the acrylic acid and the double-bond modified beta-cyclodextrin. Based on multi-covalent and non-covalent interaction, the supramolecular hydrogel has the advantage of controllably releasing plant hormones, and realizes sustainable environmental remediation by cooperating with plant remediation.

This invention belongs to the field of ecological environment monitoring technology and relates to a multi-level bioindicator method for the effective phytotoxicity of heavy metal-like substances / heavy metals. The method includes the following steps: S1. Obtaining plant samples affected by the soil or water body to be tested; S2. Measuring the activity of superoxide dismutase (SOD), gibberellin (GA), and chlorophyll content in the plant samples; S3. Based on the measured results of SOD activity, plant hormone content, and chlorophyll content, and their changes over time, classifying and assessing the effective phytotoxicity effects of heavy metal-like substances or heavy metals. This invention is the first to propose a unified three-level bioindicator method (SOD activity, GA hormone, and chlorophyll) that can effectively reflect soil pollution levels for typical heavy metal-like substances / heavy metals present in the actual environment, enabling a simpler assessment of the degree of heavy metal pollution in actual soil.

This invention relates to the field of bio-infectious agent metabolites, and discloses a bio-infectious agent metabolite for the safe improvement of agricultural products and its preparation method. The bio-infectious agent metabolite is obtained by fermenting a fermentation raw material composed of a carbon source and a nitrogen source with an immobilized composite microbial agent on an organic-inorganic composite carrier. The organic-inorganic composite carrier immobilized composite microbial agent is prepared by immobilizing the composite microbial agent onto a chitosan-sodium alginate organic embedding carrier and an inorganic adsorbent carrier using immobilization loading technology. The composite microbial agent consists of *Bacillus circinus*, *Bacillus licheniformis*, *Bacillus amyloliquefaciens*, and *Bacillus subtilis*. This invention provides a method for preparing bio-infectious agent metabolites, and yields a bio-infectious agent metabolite containing carbohydrate compounds, amino acid compounds, organic acid compounds, and plant hormones, which has a quality-improving effect on agricultural products.

This invention discloses a fiber sensor, its preparation method, and its application, belonging to the field of electrochemical sensor technology. The invention provides a flexible electrode for continuous monitoring of plant hormones, comprising a flexible conductive substrate and a fiber functional layer disposed on the end face of the flexible conductive substrate; the flexible conductive substrate includes a conductive inner core and an insulating outer layer surrounding the conductive inner core, the conductive inner core being a liquid metal gel, and the insulating outer layer comprising a polymer; the tensile stress of the flexible conductive substrate is 1~10 kPa, the plastic deformation range is 1~10 times, and the resistance change during plastic deformation is less than or equal to 4%. The fiber sensor provided by this invention, composed of the flexible electrode, can match the plastic deformation of plant growth, has low tensile stress, and maintains stable resistance during deformation, making it suitable for long-term stable monitoring of plant hormones, offering advantages of non-destructive, long-term, and stable monitoring.

The invention provides a plant growth regulator for delaying the harvesting time of cut-flower roses as well as a preparation method and application of the plant growth regulator, and belongs to the technical field of fresh cut flower cultivation. The plant growth regulator for delaying the harvesting time of the cut-flower roses comprises the following raw materials: 1-naphthylacetic acid, abscisic acid, cytokinin, a water-soluble fertilizer containing amino acid and monopotassium phosphate. According to the plant growth regulator for delaying the harvest time of the cut-flower roses, provided by the invention, the 1-naphthylacetic acid, the abscisic acid and the cytokinin are compounded together, so that the flower blooming process can be effectively delayed, and the harvest time is delayed. The plant growth regulator takes plant hormones as main raw materials, has the advantages of environmental friendliness, safety, harmlessness, low cost and the like, and further accurately regulates and controls the flower development process of the cut-flower roses by ensuring normal hormone signal transduction and development regulation and control. The growth regulator can effectively delay the harvesting time, powerfully ensures that the cut-flower roses are accurately marketed as required and the quality of the cut-flower roses is improved, and remarkably improves the production benefits and the industrial value.

The application provides a method for constructing a regulation network of a potato JA pathway in response to Cd stress. The method relates to the technical field of data processing, and comprises the following steps: obtaining multi-omics time series data of potatoes under the conditions of cadmium stress and methyl jasmonate treatment; performing signal decomposition and feature purification on the multi-omics time series data by using a weak stationary signal decomposition method based on sequence patterns to obtain a plurality of intrinsic mode components; constructing a dynamic regulation network model to capture multi-scale change patterns of response characteristics in the time dimension and the concentration dimension, thereby extracting deep features; inputting the intrinsic mode components into the trained dynamic regulation network model to output an attention weight matrix, thereby forming a regulation network representing the dynamic regulation intensity of the jasmonic acid pathway; and solving the optimal exogenous methyl jasmonate concentration for promoting biomass and inhibiting cadmium accumulation by optimizing an objective function. The application provides a high-precision model tool for analyzing the regulation mechanism of plant hormone signals under heavy metal stress.

This invention belongs to the field of ornamental horticulture cultivation technology, and relates to a cultivation method for promoting wound healing in bonsai using a plant hormone repair agent. This method addresses the problems of slow wound healing and susceptibility to infection during grafting and pruning of bonsai. It constructs a plant hormone repair agent composed of hormone components, antibacterial components, repair aids, penetrants, and a carrier, and integrates it with a complete "pretreatment—grafting—application—bandaging—maintenance" process. The repair agent creates a synergistic microenvironment of physiological regulation and antibacterial activity at the graft union and various cuts. Combined with sterile gauze and mulch wrapping, it reduces water loss and pathogen infection. Through temperature and humidity control during the healing period and light, water, and nutrient management during the growing season, the wound can complete continuous healing and gradual lignification in a short time. This invention significantly improves healing speed, infection control, grafting survival rate, and lignification stability, and is suitable for the standardized and large-scale cultivation of various bonsai materials.

The invention discloses an immune microelectrode, an immune electrochemical sensor and a preparation method and application of the immune microelectrode and the immune electrochemical sensor. An immune microelectrode prepared by modifying a micron-level metal electrode wire with a carbon-based nano material and a plant hormone antibody is used as a working electrode, and the electrochemical sensor taking the working electrode as a substrate is used for detecting plant hormones in a plant tissue micro-area. A sensor based on the electrode shows good electrode stability in the process of detecting plant hormones in a plant tissue microcell, and the detection range is large. For other interfering substances such as auxin, salicylic acid and the like in a plant body, generated response signals are greatly different from the auxin and the salicylic acid or basically have no response signals, the electrode shows good specificity, and the method for rapidly detecting the plant hormones in the plant tissue microcell through the electrode is convenient and simple. On the basis of plant tissue micro-area detection, in-situ detection can be carried out on plants.

The invention belongs to the technical field of agricultural compositions, and discloses a composition for improving low temperature resistance of rice. The composition is prepared from the following effective components in percentage by mass: 0.001 to 0.1 percent of 24-epibrassinolide, 0.1 to 10 percent of trehalose and 0.01 to 5 percent of selenomethionine. According to the invention, 24-epibrassinolide is utilized to activate a BR signal channel, and trehalose is utilized to maintain cell osmotic equilibrium and selenomethionine has an antioxidant protection effect, so that the low-temperature protection effect is enhanced together. Besides, a nano delivery technology is combined with plant hormone regulation and control, so that the low-temperature adaptive capacity of the rice in the seedling stage is remarkably improved, and an innovative scheme is provided for solving the problem of frequent occurrence of extreme low temperature under the global warming background.

The invention belongs to the technical field of microbial fermentation and food engineering, and discloses a method for promoting oleaginous microorganisms to quickly accumulate functional grease by using abscisic acid and application of the method. According to the method, the oleaginous microorganisms are subjected to fermentation culture, an exogenous compound abscisic acid is added in the fermentation culture process, and the functional grease is obtained. The final concentration of the exogenous compound added in the fermentation culture process of the abscisic acid is 0.5-3 mM. The abscisic acid can be applied to promoting the fermentation of the oleaginous microorganisms to quickly accumulate the functional grease. As an important plant hormone, abscisic acid is added as a regulator to promote the production of grease from microorganisms, which is a green production mode. And the abscisic acid has poor thermal stability, can be degraded in the oil extraction process, and does not affect the quality of the grease.

The present invention relates to a process for producing biofertilizers / biostimulants from microalgae with intensive CO2 capture, which comprises the following steps:a) preparing an inoculum;b) cultivating for the production of microalgae biomass in a window photobioreactor;c) collecting the biomass by flocculation;d) drying the biomass, and grinding and extracting a polar fraction by solvent in a two-phase system; ande) analyzing the polar fraction by liquid chromatography.The invention also relates to the extracts of wet biomass of the microalgae Scenedesmus sp., obtaining the polar fraction. This polar fraction contains amino acids, sugars, betaine derivatives, vitamin B3 (nicotinamide or nicotinic acid) and pantothenic acid (vitamin B5), the monoterpene loliolide and the tryptophan derivative indole-lactic acid (analogous to indole-acetic acid, a phytohormone of the auxin class).The polar fraction can also be used as an input in the cultivation of soybeans, corn, sugar cane, cotton and coffee, among other cultivated species.

The invention discloses a windmill-shaped alpha-Fe2O3 / Ag nano hybrid material and a preparation method and application thereof in relieving plant salt stress, and the method comprises the following steps: mixing fusiform alpha-Fe2O3 prepared by a forced hydrolysis method with silver acetate, then carrying out ball milling treatment, and then carrying out a high-temperature calcination process to obtain the windmill-shaped alpha-Fe2O3 / Ag nano hybrid material. And the windmill-shaped alpha-Fe2O3 / Ag hybrid structure formed by assembling the fusiform alpha-Fe2O3 and the Ag nanoparticles is obtained. The nano hybrid material has high specific surface area, excellent dispersity and remarkable leaf surface adhesion performance, when the nano hybrid material is applied to plant cultivation, the material can effectively relieve growth inhibition caused by salt stress, can regulate and control plant hormone signals, terpene and carbohydrate metabolism, sulfur metabolism, protein homeostasis and the like, realizes multi-channel comprehensive regulation, and has a wide application prospect. Therefore, the plant stress resistance and quality are improved.

The invention discloses a method for promoting coconut callus induction by using puerarin, and relates to the technical field of plant tissue culture. According to the method, tender inflorescences of coconut trees serve as tissue culture explants, a Y3 culture medium serves as a basic culture medium, 3 mg / L puerarin is added in the callus induction stage, it is found that the puerarin can partially replace 2, 4-D or dicamba, the use amount of the 2, 4-D or dicamba is reduced, the risk of subsequent regeneration malformation is reduced, the browning rate of the explants is reduced by 43%, the callus induction rate is increased by 16%, and the survival rate of the explants is increased. And the induction time is shortened by 30 days. Aiming at the problems of explant browning and low callus induction rate caused by high-concentration herbicide plant hormones (2, 4-D or dicamba), puerarin is added in the coconut tissue culture regeneration process, so that the dosage of 2, 4-D or dicamba is reduced, the explant browning rate is reduced, the callus induction rate is improved, the induction time is shortened, and the tissue culture regeneration method has the advantages that the tissue culture regeneration efficiency is improved, and the tissue culture regeneration cost is reduced. The method has good popularization and application prospects in the field of coconut tissue culture and rapid propagation.

The application provides an algal combined treatment method, and relates to the technical field of bioengineering, and comprises the following steps: selecting microalgae seeds; inoculating the microalgae seeds into a conical flask of a Basal culture medium; culturing under dark conditions; after the microalgae seeds reach the plateau phase, supplementing plant hormones and a nitrogen source, and culturing to obtain microalgae. The algal combined treatment method can effectively stimulate the microalgae in the plateau phase, and then can effectively improve the total carotenoid content and the lutein content.

This invention belongs to the field of biotechnology, specifically relating to the cassava transcription factor MeWRKY30 and its applications. This invention cloned the transcription factor gene of MeWRKY30 from cassava. Overexpression of this gene in cassava can improve the resistance of transgenic cassava plants to low-temperature and drought stress. The effects of qRT-PCR on cassava... MeWRKY30 Gene expression was significantly upregulated after drought and treatment with the plant hormone abscisic acid (ABA). After recovery from low temperature treatment, the malondialdehyde content of the overexpressing cassava lines decreased, wilting was reduced, root growth inhibition was lessened, and the number of cortical cells in the root tip meristem was significantly increased.

The present application relates to the field of agricultural biotechnology, and particularly relates to the role of tomato leaf roller oral secretion effector gene REPAT38 in inhibiting host plant defense. The gene REPAT38 encodes a protein with an amino acid sequence as shown in SEQ ID NO: 2. The effector induces stomatal closure in 0.5h, and inhibits the synthesis of JA, ET and ABA in 1h. In addition, the synthesis of AOC, MYC2, ACS1A, PAL, PR1, EIL2 and SRK2E, 7 plant hormone response genes, are all inhibited at different time points. Therefore, the oral secretion effector REPAT38 can weaken the defense ability of tomato host, thereby improving the feeding performance of insects. When the gene is interfered, the ability of insects to inhibit plant defense is weakened.

The invention discloses a compound fermentation inoculant for strengthening traditional Chinese medicine residue composting efficiency and a traditional Chinese medicine residue fermentation method.The compound fermentation inoculant comprises a composting function group, a nutrient production function group and a nutrient solution, in the traditional Chinese medicine residue fermentation process, the composting function group and the nutrient production function group act synergistically, the respective characteristics are exerted in different fermentation periods, and the effect of improving the traditional Chinese medicine residue composting efficiency is achieved. The degradation of lignocellulose, the fixation of nitrogen, the synthesis of flavonol and the generation of plant hormones are respectively accelerated, so that the efficiency of the composting process and the composting quality are comprehensively improved, and the planting quality of crops and the like is favorably improved.

The invention discloses a salt stress-resistant growth-promoting rhizosphere pseudomonas arcuata GLSm-Q7 and application thereof, and relates to the technical field of agricultural microorganisms, the strain number of the strain is GLSm-Q7, the strain is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.35753. The invention further discloses a preparation method of the salt stress-resistant growth-promoting rhizosphere pseudomonas arcuata GLSm-Q7. The strain provided by the invention has the capabilities of fixing nitrogen, dissolving phosphorus, dissolving potassium, producing siderophores and the like, and can improve the soil environment and promote plant growth. The strain GLSm-Q7 is inoculated under salt stress, so that the salt tolerance of the plant can be improved, the root development of the plant is promoted, and the biomass of the plant is increased; and the damage of salt stress to plants can be resisted by generating plant hormones, inducing permeation substance accumulation and improving the activity of an antioxidant enzyme system. The strain resource developed by the invention and the application system thereof provide a new technical means for plant growth promotion and stress regulation and control, and provide theoretical support for saline-alkali soil improvement and sustainable agricultural development.

Compositions and methods are provided for delivering hydrophilic functional agents to plants using lipid nanoparticles (NLPs). The NLPs comprise a hydrophilic core surrounded by a lipid shell including at least one sterol, one phospholipid, and one cationic or ionizable lipid. The hydrophilic core enables encapsulation and stabilization of water-soluble agents such as nucleic acids, peptides, small molecules, or plant hormones. The NLPs enhance uptake, protect cargo from degradation, and facilitate delivery to internal plant tissues, including meristems and vascular structures. Methods of treating plants and plant parts with the compositions are also provided.

The invention belongs to the technical field of agricultural biology, and discloses a non-tissue culture dependent transformation method for promoting effective transformation of potatoes by using growth factors. The invention discloses a reagent composition. The reagent composition comprises an infection solution and a bud inducing screening solution, wherein the infection liquid is prepared from acetosyringone, dithiothreitol and plant hormones; the bud inducing screening liquid comprises plant hormones, glufosinate-methyl and regeneration factors. The invention provides a reagent composition. The reagent composition can be used for non-tissue culture dependent genetic transformation of potatoes. By using the reagent composition, the non-tissue culture dependent genetic transformation step of the potatoes can be simplified, the conditions of chimera and false positive of transformants are reduced, and the transformation efficiency is remarkably improved.