200 results about "Plant hormone" patented technology

The invention discloses a fresh commodity after-ripening regulation and control planning method oriented to maturity grading, and relates to the technical field of food science and biologication.The method comprises the steps that physiological parameters of fresh commodities are collected in real time based on a multi-modal sensor, and the physiological parameters comprise the epidermis pigment content, the ethylene release rate, the fruit hardness and the respiration intensity; the collected physiological parameters are input into a multi-objective optimization model based on deep learning, the model fuses a metabolic kinetic equation and an LSTM time sequence prediction algorithm, and a regulation and control parameter combination adaptive to the current mature stage is output; according to the regulation and control parameter combination, the temperature, the relative humidity and the O2 / CO2 concentration gradient of the controlled atmosphere storage equipment are synchronously regulated through a distributed control system, and a plant hormone antagonist is injected through an ultrasonic atomization device; and constructing a supply chain collaborative network based on a block chain technology, binding a grading result with logistics path planning, and adjusting a temperature control strategy and a shelf life countdown parameter of the cold chain transport vehicle in real time through an edge computing node.

The invention discloses a tomato fruit cracking key regulation gene identification method, through the method, 41 key genes for regulating tomato cracking are determined and obtained for the first time, and the genes mainly participate in plant hormone synthesis, cell wall metabolism, wax and cuticle metabolism, absorption and transportation of mineral elements and water, and some transcription factors. Two regulation networks are established and comprise an ethylene-cell wall metabolic pathway and a transcription factor-water channel-mineral element. The research combines high-throughput sequencing and molecular biological techniques, enriches the cracking mechanism of tomato fruits on transcription and translation levels, and thus, has important scientific significance and application prospects.

The present disclosure provides methods of gene transformation of sunflower based on Agrobacterium-mediated gene delivery to meristem cells of fresh imbibed / germinated sunflower seeds. One embodiment of the present disclosure is a method of directly transforming a sunflower from a mature seed with a heterologous polynucleotide, comprising: generating an explant from a germinated sunflower seed by removing cotyledons and primary leaves having intact meristem and tender shoot radicles; contacting the sunflower explant comprising the cotyledon with Agrobacterium comprising a heterologous polynucleotide in an infection medium supplemented with a plant hormone using vacuum and ultrasound treatment; co-culturing the explant from a germinating sunflower plant in a culture medium lacking the plant hormone in the presence of the Agrobacterium genus; culturing the mature seed explant in a regeneration medium comprising a selective agent; and culturing the seed with the regenerated tender shoots and with the secondary roots in a culture medium lacking the selective agent.

The invention provides a haematococcus pluvialis culture medium and a culture method thereof. The culture medium comprises a nutrition proliferation culture medium, a stress induction culture medium and an accumulation strengthening culture medium which are used for segmented culture, the nutrition proliferation culture medium comprises sodium nitrate, monopotassium phosphate, potassium chloride, ferric ammonium citrate, sodium citrate and a trace element mixed solution; adding an osmotic stress agent, an oxidation inducer and plant hormones into a stress induction culture medium; and accumulating and strengthening the culture medium to remove sodium nitrate, and adding and supplementing a carbon source, a polymer penetrant and a zwitterionic stabilizer. The culture method comprises the following steps: a nutrition proliferation stage, a stress induction stage and an accumulation strengthening stage. According to the method, through a three-section culture medium and a fine regulation culture process, and through the synergistic effect of a composite stress factor and a dynamic environment signal, on the premise of ensuring relatively high activity of algae cells, bidirectional synergistic efficient improvement of biomass growth and astaxanthin accumulation is realized.

The invention relates to the technical field of conditioners, in particular to a conditioner for improving crop stress resistance and a preparation method thereof, and the conditioner comprises the following raw materials: plant hormones, trehalose, humic acid, a complex microbial inoculant, modified nano sodium alginate, modified nano zinc oxide, lactic acid, a green tea extract and a licorice extract. The modified nano-sodium alginate, the modified nano-zinc oxide and the lactic acid play a role through a synergistic effect, the modified nano-sodium alginate and nutrient substances form a modified nano-sodium alginate gel compound through physical adsorption and chemical effects, stress-resistant peptides on the surface of the compound directly activate immune systems of crops, synthesis of stress-resistant proteins is promoted, and the stress resistance of the crops is improved. The modified nano-zinc oxide enhances the absorption of nutrient substances by crop root cells, assists the activation of crop immune systems, and synergistically enhances the disease resistance and stress resistance of crops, and the lactic acid effectively stabilizes the pH value of the conditioner, relieves the oxidation pressure of crops under adverse conditions, and enhances the physiological stability of crops.

The invention provides a deuterium-labeled 3-indoleacrylic acid isotope compound and a preparation method thereof, and relates to the technical field of plant hormone detection, deuterated toluene is creatively nitrated and subjected to a ring closing reaction, then an aldehyde group is introduced, and finally hydrolysis is performed to obtain the target product deuterium-labeled 3-indoleacrylic acid isotope compound. The preparation method provided by the invention is strong in operability, relatively good in atom economy and excellent in reproducibility and stability, the isotope abundance of the prepared deuterium-labeled 3-indoleacrylic acid isotope compound can reach 98% or above, the chemical purity is 98% or above, and the deuterium-labeled 3-indoleacrylic acid isotope compound can be used as an internal standard substance for detecting plant hormones and has good application prospects. The method has important practical application value.

The invention discloses a cultivation method for improving quality and aroma of flue-cured tobaccos. The cultivation method comprises the steps that 1, the flue-cured tobaccos are planted in rainy tobacco areas in the south; 2, preparing a foliar fertilizer; the leaf fertilizer is prepared from the following components in parts by mass: 1000 parts of tobacco stem leaching liquor, 100 to 500 parts of amino acid raw powder, 50 to 100 parts of potassium nitrate, 50 to 100 parts of monopotassium phosphate, 0.005 to 0.05 part of gibberellin and 100 to 250 parts of glucose; the foliar fertilizer is prepared by adding the components in proportion and fully and uniformly mixing the components. 3, before and after topping of flue-cured tobacco plants, the prepared foliar fertilizer is used for at least one time of foliar spraying operation on fresh tobacco leaves of flue-cured tobacco. According to the quality-improving and aroma-enhancing cultivation method for the flue-cured tobacco, nitrogen, phosphorus and potassium nutrient elements and plant hormones, namely gibberellin and glucose are reasonably added into the leaf fertilizer, so that sufficient growth, natural maturity and chemical component coordination regulation of the tobacco leaves are realized, and the aroma quality of the flue-cured tobacco is improved.

The invention provides a method for regulating and controlling aroma of grape fruits through an exogenous plant growth regulator, which comprises the following steps of: spraying three exogenous plant hormones, namely methyl jasmonate, phenylalanine and strigolactones, on 'Hanxiangmi' grape fruits with strong rose aroma, and analyzing the aroma composition and content of the treated grape fruits. The result shows that the rose fragrance intensity of the'Hanxiangmi 'grapes is enhanced after the jasmonic acid methyl ester treatment; after strigolactones treatment, components such as alpha-terpilenol, beta-ionone, nonanoic acid, geraniol and the like in grape fruits are prominent, so that the treated grapes are converted into mixed fragrance type grapes with flower fragrance, fruit fragrance and grass fragrance, and the fragrance layers are rich; and the treatment effect of phenylalanine is not obvious. The invention provides a reference for the research of strigolactone in fruit quality.

The invention relates to the technical field of daily chemicals, in particular to an anti-hair-loss composition containing hexapeptide-11 and application of the anti-hair-loss composition. The composition comprises a foaming agent, a suspending agent, a hair conditioner, a foaming agent, a thickening agent, an emollient, a preservative, a PH regulator, a chelating agent, hexapeptide-11 and a solvent. The hexapeptide-11 can promote metabolism and proliferation of hair papilla cells; the ginseng and black bean hydrolysate provides plant hormone-like components; the radix scutellariae extract effectively inhibits scalp inflammation, and the three components have a remarkable synergistic effect in the aspects of regulating and controlling the hair follicle cycle and prolonging the growth period. Through synergistic fermentation conversion of probiotics, the molecular structure and skin absorption capacity of original components are improved, meanwhile, various active substances such as small molecular peptides, organic acids and B vitamins are released, and the scalp microenvironment can be improved. The hexapeptide-11 is not obviously degraded in the fermentation process, and forms a composite stable system together with the plant extract, so that the hexapeptide-11 has good pH adaptability and storage stability, and is suitable for daily scalp care.

The invention discloses a high-concentration multi-element slow-release blended fertilizer which is prepared from nutrient elements, plant hormones, ammonium bicarbonate, ammonium phosphate, potassium chloride, magnesium oxide, sub-volcanic rock, kaolin, urea, bentonite, calcium oxide, compound microorganism bacteria, diammonium hydrogen phosphate, potassium sulfate, crop waste residues, a fermentation accelerant and animal waste. The fertilizer comprises the following components in parts by weight: 2-5 parts of nutrient elements; 1-3 parts of plant hormones; and 10-15 parts of ammonium bicarbonate. The organic fertilizer and the inorganic fertilizer are combined, the product is green and environment-friendly, the utilization rate of the fertilizer is increased, the fertilizer efficiency is more remarkable, the effects of further activating soil nutrients and fertilizing soil can be achieved, root growth and cell division can be promoted, crop disease resistance can be improved, conversion and absorption of nutrient elements such as nitrogen, phosphorus and potassium in the fertilizer can be accelerated, and the fertilizer efficiency is improved. And moreover, the negative influence of the fertilizer on the environment can be effectively reduced after long-term use of the fertilizer, so that the fertilizer has good social and economic benefits.

The invention discloses a method for detecting natural plant hormones and exogenous growth regulators of vegetables, efficient separation and accurate detection of the natural plant hormones and the exogenous growth regulators are realized through combination of hydrophilic interaction chromatography and mass spectrometry, a hydrophilic interaction chromatographic column is adopted, and conditions such as mobile phase composition and flow velocity are optimized; the selectivity of polar substances is improved by utilizing hydrophilic interaction, so that compounds with similar structures achieve baseline separation, and chromatographic peak overlapping interference is avoided; the mass spectrometric detection only responds to a specific ion signal by screening characteristic parent ion / daughter ion pairs of a target object, so that matrix impurities and non-target ion interference are effectively eliminated, the false positive risk is remarkably reduced, and the detection sensitivity and specificity are improved; the combined technology is combined with the efficient separation capacity of the hydrophilic interaction chromatographic column and a multi-reaction monitoring mode, synchronous separation of multiple compounds with similar polarities in single analysis is achieved, parallel detection of dozens of target objects is supported, and the analysis time is greatly shortened.

The invention discloses a thick-stem high-yield caulis spatholobi germplasm screening and breeding method suitable for an under-forest habitat. According to the method, a standardized under-forest cultivation system is constructed, spiral winding induction synergistically mediated by plant morphology and plant hormones is implemented, lignification development of stems is enhanced by applying mild mechanical stress with specific parameters, rhizosphere microorganism regulation and systematic nutrition management are synchronously combined, and the yield of the stems is increased. And finally, a multi-dimensional comprehensive evaluation system covering the stem shape, the wood density, the active ingredient content and the under-forest weak light adaptability is established, so that an integrated technical scheme for efficiently and accurately breeding the excellent caulis spatholobi germplasm with thick stem phenotype, high flavone accumulation and strong under-forest weak light tolerance from a large number of candidate germplasm is realized.

The invention discloses a method for preparing a soil conditioner for gardenia cultivation by using immobilized compound bacteria, which comprises the following steps: extracting gardenia lignin by using gardenia waste as a raw material, and preparing an inner-layer wall material to embed a mixed bacteria solution to obtain lignin inner-layer microcapsules; preparing an outer-layer wall material through modified chitosan, and compounding and curing the inner-layer microcapsule, the hydrogenated streptomyces liquid and the outer-layer wall material to obtain the immobilized composite microbial agent. And finally, mixing the immobilized complex microbial inoculant with matrixes such as leaf mold and peat soil to obtain the modifier. Ordered release of compound bacteria is realized through dual response, functional synergy of Rafissonia, Ochrobactrum and Streptomyces hydrogenii can be enhanced through population signal molecule modification, soil nutrients can be activated, effective iron can be chelated to solve the problem of iron deficiency yellowing of cape jasmine, soil pollutants and pesticide residues are degraded, and the soil quality is improved. Pathogenic bacteria reproduction is inhibited, and rhizosphere micro-ecological balance is maintained; plant hormones can be secreted to promote the growth of the cape jasmine, and the cape jasmine is converted into more components with high medicinal value.

The invention relates to the technical field of agricultural cultivation, in particular to a breeding method of water horn seeds. The method comprises the following steps: storing dried water chestnut seeds at 8-16 DEG C for 8-24 days, and soaking the water chestnut seeds in a potassium permanganate aqueous solution for 15-25 minutes; and sowing the soaked water horns seeds in a matrix, and culturing for 18-22 days to obtain seed seedlings. According to the method provided by the invention, the germination rate of the water pod seeds is extremely high and reaches 99%; and the method is short in operation period and simple in operation steps, does not need any plant hormones and chemical substances, effectively solves the breeding problem of the water hormones, and has important significance for reducing the endangered degree of the water hormones.

The invention relates to the technical field of sugarcane smut resistance, and provides a molecular marking method for sugarcane smut resistance, which comprises a sample preparation unit, a double-sugarcane area inoculation identification unit, a whole genome association analysis unit, an SNP (Single Nucleotide Polymorphism) marker development unit, a marker verification unit and a result application unit, the output end of the sample preparation unit is connected with the input end of the double-sugarcane-area inoculation identification unit through a sample transmission channel, the double-sugarcane-area inoculation identification unit outputs phenotypic data to the whole-genome association analysis unit, and the whole-genome association analysis unit outputs remarkably associated SNP sites to the SNP marker development unit; and the SNP marker development unit outputs a specific primer to the marker verification unit. The molecular marker provided by the invention is based on the correlation between the candidate gene of the site and the known disease-resistant pathway of the plant, and the MYB transcription factor can improve or reduce the resistance level of the plant to pathogenic bacteria by regulating the synthesis of secondary metabolites, transduction of plant hormone signals, expression of defensive genes and the like.

The present application belongs to the technical field of microorganisms, and relates to a Pseudomonas jessenii JY2025 and application thereof. Pseudomonas piscicola The biological preservation number of the Pseudomonas jessenii (P. jessenii) JY2025 is CGMCC No. 37283. The strain provided by the present application has broad-spectrum antifungal activity, can significantly inhibit the growth of various plant pathogenic fungi, and has the abilities of salt and alkali tolerance, inorganic phosphorus degradation, protease secretion, siderophore secretion and indole acetic acid production, which are helpful to promote crop growth and improve soil fertility.

A lysogenic phage community, its extraction method and application. The tangential flow technology is used to proportionally isolate bacteria and phage communities from in-situ soil, and single-bacteria communities and single-phage communities are obtained through enrichment and concentration. At the same time, mitomycin C is added to the single-bacteria community for lysogenic induction, and then the tangential flow system is used again for filtration and enrichment to obtain a high-throughput lysogenic phage community. Finally, we obtain single-bacteria communities, bacteria-phage mixed communities, and bacteria-lysogenic phage mixed communities. The phage transplantation technology can promote the participation of indigenous microorganisms in the process of promoting plant growth and development. The transplanted lysogenic phages can participate in the synthesis and metabolism of host plant hormones by optimizing the microbial network structure and injecting auxiliary metabolic genes. This method provides a long-term and environmentally friendly microbial synergistic growth promotion technology for the development of green agriculture in China.

The invention relates to the field of agricultural biochemical engineering and plant protection, and particularly discloses a liquid film-forming agent for preventing crops from freezing injury due to late spring coldness and a preparation method of the liquid film-forming agent. The film-forming agent is prepared from carboxymethyl chitosan, sodium carboxymethyl cellulose, kasugamycin, brassinolide, glycol and glycerol mixed dihydric alcohol, Tween 80 and deionized water according to a specific proportion. The preparation method comprises the key processes of step-by-step dissolution, mixing, functional component addition and the like. Organic fusion of physical barrier, ice nucleus inactivation and physiological regulation is realized through multiple action mechanisms of natural polymer composite film forming, biopesticide targeted sterilization, plant hormone induced cold resistance and dihydric alcohol synergistic antifreezing. The product can provide efficient protection at a low temperature of-4 DEG C, significantly reduces the frost damage rate, has the characteristic of 28-day natural rapid degradation, and solves the technical bottlenecks of single function, difficult degradation and poor environmental compatibility of a traditional film agent.

The invention belongs to the technical field of plant breeding, and discloses a breeding method for improving the mutagenesis efficiency of stem tips of woody plants. The CRISPR-Cas9 ribonucleoprotein complex and plant hormones 6-benzyladenine, gibberellin and jasmonic acid are co-encapsulated and then accurately delivered to stem tip meristem of woody ornamental plants such as acer truncatum, pteroceltis tatarinowii, crape myrtle and tassel, and the gene mutation obtaining rate of the stem tip meristem is effectively increased; the intelligent environment control system is used for dynamically optimizing illumination, temperature, humidity and oxidative stress, activating a plant endogenous DNA repair pathway and further enhancing homologous recombination efficiency, meanwhile, through collaborative optimization of induction hormone signals and environmental stress, the stress resistance is improved, the leaf color, the plant type and the growth vigor can be accurately regulated and controlled, and the yield of the plant is increased. According to the method, efficient, accurate and stress-resistant linked woody plant stem tip mutation breeding is realized.

To provide a reagent and a methyl salicylate sensor for detecting methyl salicylate which is a plant hormone released when a plant is infected with a pathogenic fungus in the cultivation of the plant including a field crop, to provide a method for sensing methyl salicylate using the same, and to provide a method for detecting the pathogenic fungus infection of the plant.SOLUTION: A reagent for detecting methyl salicylate, comprising a terbium compound and an amine. A methyl salicylate sensor includes a methyl salicylate capture part having the reagent, and a detection part for detecting that methyl salicylate is captured by the capture part.SELECTED DRAWING: None

The invention provides a method for converting compact type callus of super bamboo reed into loose type callus, and belongs to the technical field of plant tissue culture.The method comprises the following steps that the compact type callus of the super bamboo reed is taken and transferred into a first culture medium to be subjected to subculture multiplication culture, and the loose type callus is obtained; the first culture medium comprises an MS culture medium, plant hormones, a carbon source and a coagulator; the plant hormones comprise 2, 4-D, GA and KT; or transferring the compact callus of the super bamboo reed obtained by the solid culture medium into a liquid culture medium for subculture multiplication culture, then removing the liquid culture medium, and then transferring the callus into a fresh solid culture medium for continuous culture to obtain loose callus; the liquid culture medium and the fresh solid culture medium have the same effective components. By optimizing the types of hormones in the culture medium and changing the subculture mode, the texture of the super bamboo reed callus is converted from a compact type to a loose type.

To provide a reagent for detecting methyl salicylate that is a plant hormone released when pathogen infection has arisen in plant cultivation including farm crop, and further, a methyl salicylate sensor, and a sensing method of methyl salicylate using the reagent and the sensor, and to provide a method for detecting pathogen infection in plants using the sensing method.SOLUTION: A reagent for detecting methyl salicylate contains a terbium compound doped with a rare earth element other than terbium. A methyl salicylate sensor comprises: a capturing part of methyl salicylate including the reagent; and a detection part for detecting that methyl salicylate has been captured by the capturing part.SELECTED DRAWING: None

The present invention belongs to the field of microalgae cultivation technology, and discloses a method for promoting microalgae carbon fixation and oil production by using plant hormones and saline wastewater. Microalgae are cultivated in a wastewater culture medium to which sodium chloride and plant hormones are added. The plant hormones include at least abscisic acid, which can effectively alleviate salt stress and promote microalgae oil accumulation and growth. Plant hormones also include one of auxin and gibberellin. When abscisic acid acts synergistically with auxin or gibberellin, it is more helpful to improve the oil yield of microalgae. In addition, sodium chloride, abscisic acid, 4-amino-2-methylthiopyrimidine-5-carboxamide and 4-methyl-5-thiazoleethanol can also be added to the wastewater to synergistically enhance microalgae biomass and oil accumulation. The present invention provides an effective way to reduce the cost of microalgae cultivation and realize the industrial production of microalgae biodiesel, with both environmental and energy benefits.

The invention relates to a plant cultivation method, in particular to a sterile miniature cuttage rapid propagation method for brassica napobrassica. According to the method, stem segments with buds or terminal buds of the brassica napobrassica are used as explants for asexual propagation, budlet micro cutting is performed in a sterile environment, plantlets with roots are directly induced without adding any plant hormones and inducing cluster buds and calluses, and rapid propagation of the brassica napobrassica is realized through continuous micro cutting. The method is not limited by seasons and propagation materials, seedlings can be produced in a manual control environment all the year round, the propagation speed is high, and the coefficient is large.

This invention discloses a culture medium for Antrodia camphorata mycelium, which consists of two parts: a solid medium and a liquid medium. The liquid medium contains glucose, peptone, anhydrous magnesium sulfate, and plant hormones; the plant hormones include one or more of indolebutyric acid, lignin, gibberellin, 6-benzylaminopurine, and 24-epibrassinolide. The solid medium contains carrot powder, potato powder, glucose, and agar powder; the solid medium also contains plant hormones, including one or more of indolebutyric acid, lignin, gibberellin, 6-benzylaminopurine, and 24-epibrassinolide. During cultivation, activated mycelium is first inoculated onto the solid medium to form high-quality colonies, and then inoculated onto the liquid medium for further expansion. This method significantly increases mycelium yield and the production of crude polysaccharides and crude triterpenes, providing strong technical support for the development and utilization of Antrodia camphorata.

The invention discloses application of a compound 5-HIAA in improving the transcription or expression level of a plant root auxin related gene, and belongs to the technical field of plant hormone auxin. The application of 5-hydroxyindole-3-acetic acid (5-HIAA) in improving the transcription or expression level of plant root auxin related genes is found for the first time, growth of lateral roots, root hair and adventitious roots of plants can be effectively promoted in a non-toxic range, the 5-hydroxyindole-3-acetic acid has broad-spectrum auxin activity, and the application of 5-HIAA in improving the transcription or expression level of the plant root auxin related genes is promoted. The auxin analogue disclosed by the invention can be used as an animal-derived auxin analogue to regulate and control the growth and development of a plant root system, and can be perceived by a plant auxin co-receptor TIR1 / AFBs-Aux / IAAs, so that plant rooting is promoted, and animal-plant information exchange is mediated.

The invention provides a tissue culture and rapid propagation method for dendrobium nobile, and belongs to the technical field of plant tissue culture. Comprising the following steps: taking a middle stem section of a pretreated new lateral bud as an explant, and sequentially inoculating into a bud induction culture medium for induction culture, a bud proliferation culture medium for proliferation culture and a rooting and seedling strengthening culture medium for rooting culture, the bud induction culture medium comprises the following components: plant hormones, potato powder, zeatin and activated carbon; the bud proliferation culture medium is prepared from the following components: plant hormones, potato powder, mashed apples and trehalose. The rooting and seedling strengthening culture medium comprises the following components: plant hormones, potato powder, mashed bananas and a yeast extract. According to the method disclosed by the invention, the 'Hooke summer end' is taken as a tissue culture object, the rooting rate can be obviously improved by adjusting the components of the culture medium in different stages, improving the budding rate of the explant and increasing the bud proliferation multiple, and the elongation of roots and the growth of plants are facilitated.

The invention belongs to the technical field of environmental restoration, and particularly relates to cyclodextrin cross-linked supramolecular hydrogel as well as a preparation method and application thereof. A construction unit of the supramolecular hydrogel comprises double-bond modified beta-cyclodextrin and acrylic acid interacting with the double-bond modified beta-cyclodextrin through a covalent bond; plant hormones are included in a cavity of the double-bond modified beta-cyclodextrin. The supramolecular hydrogel adhesive tape is provided with charges and cavities, can include plant hormones, and can be used together with soil improvement plants such as green manure to enhance the saline-alkali soil improvement effect by regulating and controlling the salinity of plant rhizosphere soil, improving the emergence rate, promoting plant growth and improving the environmental stress resistance of the plants. Cavities of the double-bond modified beta-cyclodextrin can effectively include plant hormones, and the supramolecular hydrogel is formed through covalent interaction between the acrylic acid and the double-bond modified beta-cyclodextrin. Based on multi-covalent and non-covalent interaction, the supramolecular hydrogel has the advantage of controllably releasing plant hormones, and realizes sustainable environmental remediation by cooperating with plant remediation.

The invention relates to the technical field of soil heavy metal remediation, in particular to a humic acid slow-release fertilizer for improving the remediation efficiency of barbadosnut on cadmium-contaminated soil and a preparation method of the humic acid slow-release fertilizer. The fertilizer specifically comprises the following components: polyvinyl alcohol, span 80, urea, calcium hydrophosphate, potassium sulfate, magnesium oxide, humic acid, DA-6, IAA, IBA, EGTA, corn straw compost and the like. The humic acid slow-release fertilizer and the application method thereof, provided by the invention, have remarkable beneficial effects in the aspect of improving the remediation efficiency of the barbadosnut on the cadmium-polluted soil. According to the slow-release fertilizer, humic acid, plant hormones, a metal chelating agent and nutrient elements are scientifically proportioned, and corn straw compost is combined as an organic carrier, so that slow release and efficient utilization of nutrients are realized. The high-activity extraction process of humic acid ensures the strong complexing ability of rich functional groups and heavy metals, and significantly enhances the cadmium absorption and transport ability of jatropha curcas.

This invention belongs to the field of ecological environment monitoring technology and relates to a multi-level bioindicator method for the effective phytotoxicity of heavy metal-like substances / heavy metals. The method includes the following steps: S1. Obtaining plant samples affected by the soil or water body to be tested; S2. Measuring the activity of superoxide dismutase (SOD), gibberellin (GA), and chlorophyll content in the plant samples; S3. Based on the measured results of SOD activity, plant hormone content, and chlorophyll content, and their changes over time, classifying and assessing the effective phytotoxicity effects of heavy metal-like substances or heavy metals. This invention is the first to propose a unified three-level bioindicator method (SOD activity, GA hormone, and chlorophyll) that can effectively reflect soil pollution levels for typical heavy metal-like substances / heavy metals present in the actual environment, enabling a simpler assessment of the degree of heavy metal pollution in actual soil.