146 results about "Plant hormone" patented technology

The invention discloses a fresh commodity after-ripening regulation and control planning method oriented to maturity grading, and relates to the technical field of food science and biologication.The method comprises the steps that physiological parameters of fresh commodities are collected in real time based on a multi-modal sensor, and the physiological parameters comprise the epidermis pigment content, the ethylene release rate, the fruit hardness and the respiration intensity; the collected physiological parameters are input into a multi-objective optimization model based on deep learning, the model fuses a metabolic kinetic equation and an LSTM time sequence prediction algorithm, and a regulation and control parameter combination adaptive to the current mature stage is output; according to the regulation and control parameter combination, the temperature, the relative humidity and the O2 / CO2 concentration gradient of the controlled atmosphere storage equipment are synchronously regulated through a distributed control system, and a plant hormone antagonist is injected through an ultrasonic atomization device; and constructing a supply chain collaborative network based on a block chain technology, binding a grading result with logistics path planning, and adjusting a temperature control strategy and a shelf life countdown parameter of the cold chain transport vehicle in real time through an edge computing node.

The present disclosure provides methods of gene transformation of sunflower based on Agrobacterium-mediated gene delivery to meristem cells of fresh imbibed / germinated sunflower seeds. One embodiment of the present disclosure is a method of directly transforming a sunflower from a mature seed with a heterologous polynucleotide, comprising: generating an explant from a germinated sunflower seed by removing cotyledons and primary leaves having intact meristem and tender shoot radicles; contacting the sunflower explant comprising the cotyledon with Agrobacterium comprising a heterologous polynucleotide in an infection medium supplemented with a plant hormone using vacuum and ultrasound treatment; co-culturing the explant from a germinating sunflower plant in a culture medium lacking the plant hormone in the presence of the Agrobacterium genus; culturing the mature seed explant in a regeneration medium comprising a selective agent; and culturing the seed with the regenerated tender shoots and with the secondary roots in a culture medium lacking the selective agent.

The invention provides a method for regulating and controlling aroma of grape fruits through an exogenous plant growth regulator, which comprises the following steps of: spraying three exogenous plant hormones, namely methyl jasmonate, phenylalanine and strigolactones, on 'Hanxiangmi' grape fruits with strong rose aroma, and analyzing the aroma composition and content of the treated grape fruits. The result shows that the rose fragrance intensity of the'Hanxiangmi 'grapes is enhanced after the jasmonic acid methyl ester treatment; after strigolactones treatment, components such as alpha-terpilenol, beta-ionone, nonanoic acid, geraniol and the like in grape fruits are prominent, so that the treated grapes are converted into mixed fragrance type grapes with flower fragrance, fruit fragrance and grass fragrance, and the fragrance layers are rich; and the treatment effect of phenylalanine is not obvious. The invention provides a reference for the research of strigolactone in fruit quality.

The invention relates to the technical field of daily chemicals, in particular to an anti-hair-loss composition containing hexapeptide-11 and application of the anti-hair-loss composition. The composition comprises a foaming agent, a suspending agent, a hair conditioner, a foaming agent, a thickening agent, an emollient, a preservative, a PH regulator, a chelating agent, hexapeptide-11 and a solvent. The hexapeptide-11 can promote metabolism and proliferation of hair papilla cells; the ginseng and black bean hydrolysate provides plant hormone-like components; the radix scutellariae extract effectively inhibits scalp inflammation, and the three components have a remarkable synergistic effect in the aspects of regulating and controlling the hair follicle cycle and prolonging the growth period. Through synergistic fermentation conversion of probiotics, the molecular structure and skin absorption capacity of original components are improved, meanwhile, various active substances such as small molecular peptides, organic acids and B vitamins are released, and the scalp microenvironment can be improved. The hexapeptide-11 is not obviously degraded in the fermentation process, and forms a composite stable system together with the plant extract, so that the hexapeptide-11 has good pH adaptability and storage stability, and is suitable for daily scalp care.

The invention discloses a method for detecting natural plant hormones and exogenous growth regulators of vegetables, efficient separation and accurate detection of the natural plant hormones and the exogenous growth regulators are realized through combination of hydrophilic interaction chromatography and mass spectrometry, a hydrophilic interaction chromatographic column is adopted, and conditions such as mobile phase composition and flow velocity are optimized; the selectivity of polar substances is improved by utilizing hydrophilic interaction, so that compounds with similar structures achieve baseline separation, and chromatographic peak overlapping interference is avoided; the mass spectrometric detection only responds to a specific ion signal by screening characteristic parent ion / daughter ion pairs of a target object, so that matrix impurities and non-target ion interference are effectively eliminated, the false positive risk is remarkably reduced, and the detection sensitivity and specificity are improved; the combined technology is combined with the efficient separation capacity of the hydrophilic interaction chromatographic column and a multi-reaction monitoring mode, synchronous separation of multiple compounds with similar polarities in single analysis is achieved, parallel detection of dozens of target objects is supported, and the analysis time is greatly shortened.

The invention discloses a thick-stem high-yield caulis spatholobi germplasm screening and breeding method suitable for an under-forest habitat. According to the method, a standardized under-forest cultivation system is constructed, spiral winding induction synergistically mediated by plant morphology and plant hormones is implemented, lignification development of stems is enhanced by applying mild mechanical stress with specific parameters, rhizosphere microorganism regulation and systematic nutrition management are synchronously combined, and the yield of the stems is increased. And finally, a multi-dimensional comprehensive evaluation system covering the stem shape, the wood density, the active ingredient content and the under-forest weak light adaptability is established, so that an integrated technical scheme for efficiently and accurately breeding the excellent caulis spatholobi germplasm with thick stem phenotype, high flavone accumulation and strong under-forest weak light tolerance from a large number of candidate germplasm is realized.

The invention discloses a method for preparing a soil conditioner for gardenia cultivation by using immobilized compound bacteria, which comprises the following steps: extracting gardenia lignin by using gardenia waste as a raw material, and preparing an inner-layer wall material to embed a mixed bacteria solution to obtain lignin inner-layer microcapsules; preparing an outer-layer wall material through modified chitosan, and compounding and curing the inner-layer microcapsule, the hydrogenated streptomyces liquid and the outer-layer wall material to obtain the immobilized composite microbial agent. And finally, mixing the immobilized complex microbial inoculant with matrixes such as leaf mold and peat soil to obtain the modifier. Ordered release of compound bacteria is realized through dual response, functional synergy of Rafissonia, Ochrobactrum and Streptomyces hydrogenii can be enhanced through population signal molecule modification, soil nutrients can be activated, effective iron can be chelated to solve the problem of iron deficiency yellowing of cape jasmine, soil pollutants and pesticide residues are degraded, and the soil quality is improved. Pathogenic bacteria reproduction is inhibited, and rhizosphere micro-ecological balance is maintained; plant hormones can be secreted to promote the growth of the cape jasmine, and the cape jasmine is converted into more components with high medicinal value.

The invention relates to the technical field of agricultural cultivation, in particular to a breeding method of water horn seeds. The method comprises the following steps: storing dried water chestnut seeds at 8-16 DEG C for 8-24 days, and soaking the water chestnut seeds in a potassium permanganate aqueous solution for 15-25 minutes; and sowing the soaked water horns seeds in a matrix, and culturing for 18-22 days to obtain seed seedlings. According to the method provided by the invention, the germination rate of the water pod seeds is extremely high and reaches 99%; and the method is short in operation period and simple in operation steps, does not need any plant hormones and chemical substances, effectively solves the breeding problem of the water hormones, and has important significance for reducing the endangered degree of the water hormones.

The invention relates to the technical field of sugarcane smut resistance, and provides a molecular marking method for sugarcane smut resistance, which comprises a sample preparation unit, a double-sugarcane area inoculation identification unit, a whole genome association analysis unit, an SNP (Single Nucleotide Polymorphism) marker development unit, a marker verification unit and a result application unit, the output end of the sample preparation unit is connected with the input end of the double-sugarcane-area inoculation identification unit through a sample transmission channel, the double-sugarcane-area inoculation identification unit outputs phenotypic data to the whole-genome association analysis unit, and the whole-genome association analysis unit outputs remarkably associated SNP sites to the SNP marker development unit; and the SNP marker development unit outputs a specific primer to the marker verification unit. The molecular marker provided by the invention is based on the correlation between the candidate gene of the site and the known disease-resistant pathway of the plant, and the MYB transcription factor can improve or reduce the resistance level of the plant to pathogenic bacteria by regulating the synthesis of secondary metabolites, transduction of plant hormone signals, expression of defensive genes and the like.

The present application belongs to the technical field of microorganisms, and relates to a Pseudomonas jessenii JY2025 and application thereof. Pseudomonas piscicola The biological preservation number of the Pseudomonas jessenii (P. jessenii) JY2025 is CGMCC No. 37283. The strain provided by the present application has broad-spectrum antifungal activity, can significantly inhibit the growth of various plant pathogenic fungi, and has the abilities of salt and alkali tolerance, inorganic phosphorus degradation, protease secretion, siderophore secretion and indole acetic acid production, which are helpful to promote crop growth and improve soil fertility.

The invention relates to the field of agricultural biochemical engineering and plant protection, and particularly discloses a liquid film-forming agent for preventing crops from freezing injury due to late spring coldness and a preparation method of the liquid film-forming agent. The film-forming agent is prepared from carboxymethyl chitosan, sodium carboxymethyl cellulose, kasugamycin, brassinolide, glycol and glycerol mixed dihydric alcohol, Tween 80 and deionized water according to a specific proportion. The preparation method comprises the key processes of step-by-step dissolution, mixing, functional component addition and the like. Organic fusion of physical barrier, ice nucleus inactivation and physiological regulation is realized through multiple action mechanisms of natural polymer composite film forming, biopesticide targeted sterilization, plant hormone induced cold resistance and dihydric alcohol synergistic antifreezing. The product can provide efficient protection at a low temperature of-4 DEG C, significantly reduces the frost damage rate, has the characteristic of 28-day natural rapid degradation, and solves the technical bottlenecks of single function, difficult degradation and poor environmental compatibility of a traditional film agent.

The invention belongs to the technical field of plant breeding, and discloses a breeding method for improving the mutagenesis efficiency of stem tips of woody plants. The CRISPR-Cas9 ribonucleoprotein complex and plant hormones 6-benzyladenine, gibberellin and jasmonic acid are co-encapsulated and then accurately delivered to stem tip meristem of woody ornamental plants such as acer truncatum, pteroceltis tatarinowii, crape myrtle and tassel, and the gene mutation obtaining rate of the stem tip meristem is effectively increased; the intelligent environment control system is used for dynamically optimizing illumination, temperature, humidity and oxidative stress, activating a plant endogenous DNA repair pathway and further enhancing homologous recombination efficiency, meanwhile, through collaborative optimization of induction hormone signals and environmental stress, the stress resistance is improved, the leaf color, the plant type and the growth vigor can be accurately regulated and controlled, and the yield of the plant is increased. According to the method, efficient, accurate and stress-resistant linked woody plant stem tip mutation breeding is realized.

To provide a reagent and a methyl salicylate sensor for detecting methyl salicylate which is a plant hormone released when a plant is infected with a pathogenic fungus in the cultivation of the plant including a field crop, to provide a method for sensing methyl salicylate using the same, and to provide a method for detecting the pathogenic fungus infection of the plant.SOLUTION: A reagent for detecting methyl salicylate, comprising a terbium compound and an amine. A methyl salicylate sensor includes a methyl salicylate capture part having the reagent, and a detection part for detecting that methyl salicylate is captured by the capture part.SELECTED DRAWING: None

The invention provides a method for converting compact type callus of super bamboo reed into loose type callus, and belongs to the technical field of plant tissue culture.The method comprises the following steps that the compact type callus of the super bamboo reed is taken and transferred into a first culture medium to be subjected to subculture multiplication culture, and the loose type callus is obtained; the first culture medium comprises an MS culture medium, plant hormones, a carbon source and a coagulator; the plant hormones comprise 2, 4-D, GA and KT; or transferring the compact callus of the super bamboo reed obtained by the solid culture medium into a liquid culture medium for subculture multiplication culture, then removing the liquid culture medium, and then transferring the callus into a fresh solid culture medium for continuous culture to obtain loose callus; the liquid culture medium and the fresh solid culture medium have the same effective components. By optimizing the types of hormones in the culture medium and changing the subculture mode, the texture of the super bamboo reed callus is converted from a compact type to a loose type.

The present invention belongs to the field of microalgae cultivation technology, and discloses a method for promoting microalgae carbon fixation and oil production by using plant hormones and saline wastewater. Microalgae are cultivated in a wastewater culture medium to which sodium chloride and plant hormones are added. The plant hormones include at least abscisic acid, which can effectively alleviate salt stress and promote microalgae oil accumulation and growth. Plant hormones also include one of auxin and gibberellin. When abscisic acid acts synergistically with auxin or gibberellin, it is more helpful to improve the oil yield of microalgae. In addition, sodium chloride, abscisic acid, 4-amino-2-methylthiopyrimidine-5-carboxamide and 4-methyl-5-thiazoleethanol can also be added to the wastewater to synergistically enhance microalgae biomass and oil accumulation. The present invention provides an effective way to reduce the cost of microalgae cultivation and realize the industrial production of microalgae biodiesel, with both environmental and energy benefits.

The invention relates to a plant cultivation method, in particular to a sterile miniature cuttage rapid propagation method for brassica napobrassica. According to the method, stem segments with buds or terminal buds of the brassica napobrassica are used as explants for asexual propagation, budlet micro cutting is performed in a sterile environment, plantlets with roots are directly induced without adding any plant hormones and inducing cluster buds and calluses, and rapid propagation of the brassica napobrassica is realized through continuous micro cutting. The method is not limited by seasons and propagation materials, seedlings can be produced in a manual control environment all the year round, the propagation speed is high, and the coefficient is large.

This invention discloses a culture medium for Antrodia camphorata mycelium, which consists of two parts: a solid medium and a liquid medium. The liquid medium contains glucose, peptone, anhydrous magnesium sulfate, and plant hormones; the plant hormones include one or more of indolebutyric acid, lignin, gibberellin, 6-benzylaminopurine, and 24-epibrassinolide. The solid medium contains carrot powder, potato powder, glucose, and agar powder; the solid medium also contains plant hormones, including one or more of indolebutyric acid, lignin, gibberellin, 6-benzylaminopurine, and 24-epibrassinolide. During cultivation, activated mycelium is first inoculated onto the solid medium to form high-quality colonies, and then inoculated onto the liquid medium for further expansion. This method significantly increases mycelium yield and the production of crude polysaccharides and crude triterpenes, providing strong technical support for the development and utilization of Antrodia camphorata.

The invention belongs to the technical field of environmental restoration, and particularly relates to cyclodextrin cross-linked supramolecular hydrogel as well as a preparation method and application thereof. A construction unit of the supramolecular hydrogel comprises double-bond modified beta-cyclodextrin and acrylic acid interacting with the double-bond modified beta-cyclodextrin through a covalent bond; plant hormones are included in a cavity of the double-bond modified beta-cyclodextrin. The supramolecular hydrogel adhesive tape is provided with charges and cavities, can include plant hormones, and can be used together with soil improvement plants such as green manure to enhance the saline-alkali soil improvement effect by regulating and controlling the salinity of plant rhizosphere soil, improving the emergence rate, promoting plant growth and improving the environmental stress resistance of the plants. Cavities of the double-bond modified beta-cyclodextrin can effectively include plant hormones, and the supramolecular hydrogel is formed through covalent interaction between the acrylic acid and the double-bond modified beta-cyclodextrin. Based on multi-covalent and non-covalent interaction, the supramolecular hydrogel has the advantage of controllably releasing plant hormones, and realizes sustainable environmental remediation by cooperating with plant remediation.

The invention relates to the technical field of soil heavy metal remediation, in particular to a humic acid slow-release fertilizer for improving the remediation efficiency of barbadosnut on cadmium-contaminated soil and a preparation method of the humic acid slow-release fertilizer. The fertilizer specifically comprises the following components: polyvinyl alcohol, span 80, urea, calcium hydrophosphate, potassium sulfate, magnesium oxide, humic acid, DA-6, IAA, IBA, EGTA, corn straw compost and the like. The humic acid slow-release fertilizer and the application method thereof, provided by the invention, have remarkable beneficial effects in the aspect of improving the remediation efficiency of the barbadosnut on the cadmium-polluted soil. According to the slow-release fertilizer, humic acid, plant hormones, a metal chelating agent and nutrient elements are scientifically proportioned, and corn straw compost is combined as an organic carrier, so that slow release and efficient utilization of nutrients are realized. The high-activity extraction process of humic acid ensures the strong complexing ability of rich functional groups and heavy metals, and significantly enhances the cadmium absorption and transport ability of jatropha curcas.

This invention belongs to the field of ecological environment monitoring technology and relates to a multi-level bioindicator method for the effective phytotoxicity of heavy metal-like substances / heavy metals. The method includes the following steps: S1. Obtaining plant samples affected by the soil or water body to be tested; S2. Measuring the activity of superoxide dismutase (SOD), gibberellin (GA), and chlorophyll content in the plant samples; S3. Based on the measured results of SOD activity, plant hormone content, and chlorophyll content, and their changes over time, classifying and assessing the effective phytotoxicity effects of heavy metal-like substances or heavy metals. This invention is the first to propose a unified three-level bioindicator method (SOD activity, GA hormone, and chlorophyll) that can effectively reflect soil pollution levels for typical heavy metal-like substances / heavy metals present in the actual environment, enabling a simpler assessment of the degree of heavy metal pollution in actual soil.

The invention discloses a preparation method of brassinolide derived carbon quantum dots and application of the brassinolide derived carbon quantum dots in plant hormone receptor detection. According to the method, BRs is used as a precursor, CQDs with a BRs semi-reserved structure are prepared through co-carbonization with citric acid, and the nano material with the particle size of 3.1 + / -0.5 nm and the surface rich in hydroxyl / keto is obtained after dialysis and purification. When the CQDs are combined with the BRs receptors, the fluorescence intensity of the CQDs is linearly enhanced along with the concentration of the receptors, the nano sensor constructed based on the CQDs can realize rapid quantitative detection of the BRs receptors in rice leaves, the linear range is 0.01-4.0 mg / mL, the detection limit is as low as 0.003 mg / mL, and the sensitivity is improved by 10 times compared with that of ELISA (Enzyme-Linked Immunosorbent Assay). By detecting the receptor expression quantity of a wild type and BRs receptor overexpression strain in a germination period, a heading period and a mature period, it is verified that the result of the strain is highly consistent with that of Western Blot, and the detection time is shortened to 30 minutes. The method does not need targeted modification, has the advantages of high specificity, interference resistance and low cost, and provides an efficient detection tool for rice molecular breeding and stress resistance regulation.

This invention relates to the field of bio-infectious agent metabolites, and discloses a bio-infectious agent metabolite for the safe improvement of agricultural products and its preparation method. The bio-infectious agent metabolite is obtained by fermenting a fermentation raw material composed of a carbon source and a nitrogen source with an immobilized composite microbial agent on an organic-inorganic composite carrier. The organic-inorganic composite carrier immobilized composite microbial agent is prepared by immobilizing the composite microbial agent onto a chitosan-sodium alginate organic embedding carrier and an inorganic adsorbent carrier using immobilization loading technology. The composite microbial agent consists of *Bacillus circinus*, *Bacillus licheniformis*, *Bacillus amyloliquefaciens*, and *Bacillus subtilis*. This invention provides a method for preparing bio-infectious agent metabolites, and yields a bio-infectious agent metabolite containing carbohydrate compounds, amino acid compounds, organic acid compounds, and plant hormones, which has a quality-improving effect on agricultural products.

This invention discloses a fiber sensor, its preparation method, and its application, belonging to the field of electrochemical sensor technology. The invention provides a flexible electrode for continuous monitoring of plant hormones, comprising a flexible conductive substrate and a fiber functional layer disposed on the end face of the flexible conductive substrate; the flexible conductive substrate includes a conductive inner core and an insulating outer layer surrounding the conductive inner core, the conductive inner core being a liquid metal gel, and the insulating outer layer comprising a polymer; the tensile stress of the flexible conductive substrate is 1~10 kPa, the plastic deformation range is 1~10 times, and the resistance change during plastic deformation is less than or equal to 4%. The fiber sensor provided by this invention, composed of the flexible electrode, can match the plastic deformation of plant growth, has low tensile stress, and maintains stable resistance during deformation, making it suitable for long-term stable monitoring of plant hormones, offering advantages of non-destructive, long-term, and stable monitoring.

The invention provides a plant growth regulator for delaying the harvesting time of cut-flower roses as well as a preparation method and application of the plant growth regulator, and belongs to the technical field of fresh cut flower cultivation. The plant growth regulator for delaying the harvesting time of the cut-flower roses comprises the following raw materials: 1-naphthylacetic acid, abscisic acid, cytokinin, a water-soluble fertilizer containing amino acid and monopotassium phosphate. According to the plant growth regulator for delaying the harvest time of the cut-flower roses, provided by the invention, the 1-naphthylacetic acid, the abscisic acid and the cytokinin are compounded together, so that the flower blooming process can be effectively delayed, and the harvest time is delayed. The plant growth regulator takes plant hormones as main raw materials, has the advantages of environmental friendliness, safety, harmlessness, low cost and the like, and further accurately regulates and controls the flower development process of the cut-flower roses by ensuring normal hormone signal transduction and development regulation and control. The growth regulator can effectively delay the harvesting time, powerfully ensures that the cut-flower roses are accurately marketed as required and the quality of the cut-flower roses is improved, and remarkably improves the production benefits and the industrial value.

The invention discloses a novel vegetable health magnesium resistance inducer as well as a preparation method and application thereof, the main components of the vegetable health magnesium resistance inducer are trimethylglycine (GB) and magnesium oxide nanoparticles (MgONPs), and the components have the characteristics of no toxicity, harmlessness, no pollution and the like. The invention finds that the'vegetable health magnesium 'resistance inducer can activate plant hormone signals, enhance plant immunity and resist virus diseases. The composition can be sprayed to vegetable crops before the high-incidence period of vegetable virus diseases to prevent and treat virus diseases, is simple and convenient, has strong operability, and can effectively improve the broad-spectrum resistance of plants to various vegetable virus diseases. Compared with traditional pesticides, the composition is more green and safer, meets the requirements of sustainable and healthy development of agriculture, and has huge application potential.

The application provides a method for constructing a regulation network of a potato JA pathway in response to Cd stress. The method relates to the technical field of data processing, and comprises the following steps: obtaining multi-omics time series data of potatoes under the conditions of cadmium stress and methyl jasmonate treatment; performing signal decomposition and feature purification on the multi-omics time series data by using a weak stationary signal decomposition method based on sequence patterns to obtain a plurality of intrinsic mode components; constructing a dynamic regulation network model to capture multi-scale change patterns of response characteristics in the time dimension and the concentration dimension, thereby extracting deep features; inputting the intrinsic mode components into the trained dynamic regulation network model to output an attention weight matrix, thereby forming a regulation network representing the dynamic regulation intensity of the jasmonic acid pathway; and solving the optimal exogenous methyl jasmonate concentration for promoting biomass and inhibiting cadmium accumulation by optimizing an objective function. The application provides a high-precision model tool for analyzing the regulation mechanism of plant hormone signals under heavy metal stress.

The invention discloses application of Cladosporium halotolerans in plant growth promotion, and belongs to the technical field of microorganisms, the Cladosporium halotolerans is Cladosporium halotolerans 550W1, and is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.41943. The invention further discloses a preparation method of the Cladosporium halotolerans. The application of the Cladosporium halotolerans in plant growth promotion belongs to the technical field of microorganisms. According to the salt-tolerant cladosporium sp. 550W1 provided by the invention, plant growth can be promoted by synthesizing auxin, gibberellin, cytokinin and other plant hormones; meanwhile, the salt-tolerant cladosporium sp. 550W1 has the outstanding advantages of being high in environmental adaptability, environmentally friendly, high in safety and the like, and an excellent core strain resource is provided for developing novel microbial fertilizers and promoting green sustainable agriculture.

This invention belongs to the field of ornamental horticulture cultivation technology, and relates to a cultivation method for promoting wound healing in bonsai using a plant hormone repair agent. This method addresses the problems of slow wound healing and susceptibility to infection during grafting and pruning of bonsai. It constructs a plant hormone repair agent composed of hormone components, antibacterial components, repair aids, penetrants, and a carrier, and integrates it with a complete "pretreatment—grafting—application—bandaging—maintenance" process. The repair agent creates a synergistic microenvironment of physiological regulation and antibacterial activity at the graft union and various cuts. Combined with sterile gauze and mulch wrapping, it reduces water loss and pathogen infection. Through temperature and humidity control during the healing period and light, water, and nutrient management during the growing season, the wound can complete continuous healing and gradual lignification in a short time. This invention significantly improves healing speed, infection control, grafting survival rate, and lignification stability, and is suitable for the standardized and large-scale cultivation of various bonsai materials.

The invention discloses an immune microelectrode, an immune electrochemical sensor and a preparation method and application of the immune microelectrode and the immune electrochemical sensor. An immune microelectrode prepared by modifying a micron-level metal electrode wire with a carbon-based nano material and a plant hormone antibody is used as a working electrode, and the electrochemical sensor taking the working electrode as a substrate is used for detecting plant hormones in a plant tissue micro-area. A sensor based on the electrode shows good electrode stability in the process of detecting plant hormones in a plant tissue microcell, and the detection range is large. For other interfering substances such as auxin, salicylic acid and the like in a plant body, generated response signals are greatly different from the auxin and the salicylic acid or basically have no response signals, the electrode shows good specificity, and the method for rapidly detecting the plant hormones in the plant tissue microcell through the electrode is convenient and simple. On the basis of plant tissue micro-area detection, in-situ detection can be carried out on plants.

The invention relates to the technical field of ecological management, and discloses a method for planting suaeda salsa in saline-alkali soil, which comprises the following steps: seed pretreatment: freezing suaeda salsa seeds in a low-temperature environment of-5 DEG C to 0 DEG C for 12-24 hours, then quickly transferring the seeds to an environment of 25 DEG C to 30 DEG C, unfreezing and activating, and taking out the seeds; then putting into a mixed solution containing 0.5%-1% of biological enzyme and 0.3%-0.5% of plant hormone, and soaking for 8-12 hours; soil improvement: paving a layer of mixture of organic fertilizer and saline-alkali tolerant microbial agent with the thickness of 5-10cm on the surface of the saline-alkali soil. Through an innovative seed pretreatment method, low-temperature freezing and unfreezing activation are combined with biological enzyme and plant hormone solution soaking, seed dormancy is broken, internal physiological metabolism of seeds is activated, and the seeds germinate more quickly and neatly. The organic fertilizer and the saline-alkaline tolerant microbial agent in scientific proportion are matched for soil improvement, so that the saline-alkaline degree of the soil is effectively reduced, and the microbial community structure of the soil is improved.