249 results about "Plant hormone" patented technology

The invention discloses a fresh commodity after-ripening regulation and control planning method oriented to maturity grading, and relates to the technical field of food science and biologication.The method comprises the steps that physiological parameters of fresh commodities are collected in real time based on a multi-modal sensor, and the physiological parameters comprise the epidermis pigment content, the ethylene release rate, the fruit hardness and the respiration intensity; the collected physiological parameters are input into a multi-objective optimization model based on deep learning, the model fuses a metabolic kinetic equation and an LSTM time sequence prediction algorithm, and a regulation and control parameter combination adaptive to the current mature stage is output; according to the regulation and control parameter combination, the temperature, the relative humidity and the O2 / CO2 concentration gradient of the controlled atmosphere storage equipment are synchronously regulated through a distributed control system, and a plant hormone antagonist is injected through an ultrasonic atomization device; and constructing a supply chain collaborative network based on a block chain technology, binding a grading result with logistics path planning, and adjusting a temperature control strategy and a shelf life countdown parameter of the cold chain transport vehicle in real time through an edge computing node.

The invention discloses a tomato fruit cracking key regulation gene identification method, through the method, 41 key genes for regulating tomato cracking are determined and obtained for the first time, and the genes mainly participate in plant hormone synthesis, cell wall metabolism, wax and cuticle metabolism, absorption and transportation of mineral elements and water, and some transcription factors. Two regulation networks are established and comprise an ethylene-cell wall metabolic pathway and a transcription factor-water channel-mineral element. The research combines high-throughput sequencing and molecular biological techniques, enriches the cracking mechanism of tomato fruits on transcription and translation levels, and thus, has important scientific significance and application prospects.

The invention belongs to the technical field of biology, and discloses a method and application for regulating and controlling the fermentation process of schizochytrium limacinum through plant hormones to improve the oil titer, and the method is characterized in that in the fermentation process of schizochytrium limacinum, one or more plant hormones are added into a culture medium to promote accumulation of oil. Plant hormones are added into a fermentation culture medium of schizochytrium limacinum, the plant hormones comprise gibberellin (GB), 6-benayl aminopurine (6-BAP), forchlorfenuron (CPPU) and brassinolide (BR), and the plant hormones promote grease accumulation by adjusting activity of enzymes related to fatty acid synthesis, so that the plant hormones can adjust metabolic pathways of the schizochytrium limacinum and promote grease accumulation. The synthesis efficiency and the yield of the grease are obviously improved.

The invention discloses a ganoderma lucidum edgeworthia chrysantha promoting agent for promoting efficient edgeworthia chrysantha of aquilaria plants, and belongs to the technical field of edgeworthia chrysantha of aquilaria plants. The component A is prepared from the following components in parts by weight: 20 to 40 parts of aspergillus niger microbial fermentation liquor, 15 to 30 parts of verticillium fulvum microbial fermentation liquor, 10 to 15 parts of fusarium oxysporum microbial fermentation liquor, 5 to 10 parts of ganoderma lucidum extract, 6 to 8 parts of seaweed extract, 2 to 8 parts of malic acid, 3 to 6 parts of fructose, 1 to 3 parts of pectinase and 0.5 to 2 parts of cellulase. Through the synergistic effect of the component A and the component B, the agilawood formation rate and quality of agilawood are effectively improved, the synergistic effect of microorganisms stimulates the agilawood formation potential of plants, accurate regulation and control of the plant hormone xylan emulsion provide conditions for synthesis and accumulation of agilawood substances, and compared with a traditional agilawood formation method, the agilawood formation method has the advantages that the agilawood formation rate is increased, and the agilawood formation quality is improved. According to the method, the agilawood with higher quality can be obtained within shorter time, and the yield is increased.

The present disclosure provides methods of gene transformation of sunflower based on Agrobacterium-mediated gene delivery to meristem cells of fresh imbibed / germinated sunflower seeds. One embodiment of the present disclosure is a method of directly transforming a sunflower from a mature seed with a heterologous polynucleotide, comprising: generating an explant from a germinated sunflower seed by removing cotyledons and primary leaves having intact meristem and tender shoot radicles; contacting the sunflower explant comprising the cotyledon with Agrobacterium comprising a heterologous polynucleotide in an infection medium supplemented with a plant hormone using vacuum and ultrasound treatment; co-culturing the explant from a germinating sunflower plant in a culture medium lacking the plant hormone in the presence of the Agrobacterium genus; culturing the mature seed explant in a regeneration medium comprising a selective agent; and culturing the seed with the regenerated tender shoots and with the secondary roots in a culture medium lacking the selective agent.

The invention belongs to the technical field of bioengineering, and particularly relates to application of plant hormones in inhibition of fungal growth and inhibition of mycotoxin synthesis. The invention provides an application of a plant hormone in inhibition of fungal growth and inhibition of synthesis of mycotoxin, the plant hormone is methyl jasmonate, the mycotoxin is deoxynivalenol, and the final concentration of the methyl jasmonate is 30-4000 [mu] M. The invention provides an inhibitor for fusarium graminearum growth and DON synthesis, and provides a botanical inhibitor for formulating a safe, pollution-free and green wheat scab prevention and control strategy.

The invention provides a haematococcus pluvialis culture medium and a culture method thereof. The culture medium comprises a nutrition proliferation culture medium, a stress induction culture medium and an accumulation strengthening culture medium which are used for segmented culture, the nutrition proliferation culture medium comprises sodium nitrate, monopotassium phosphate, potassium chloride, ferric ammonium citrate, sodium citrate and a trace element mixed solution; adding an osmotic stress agent, an oxidation inducer and plant hormones into a stress induction culture medium; and accumulating and strengthening the culture medium to remove sodium nitrate, and adding and supplementing a carbon source, a polymer penetrant and a zwitterionic stabilizer. The culture method comprises the following steps: a nutrition proliferation stage, a stress induction stage and an accumulation strengthening stage. According to the method, through a three-section culture medium and a fine regulation culture process, and through the synergistic effect of a composite stress factor and a dynamic environment signal, on the premise of ensuring relatively high activity of algae cells, bidirectional synergistic efficient improvement of biomass growth and astaxanthin accumulation is realized.

The invention provides application of mitochondrial acid 5 in preparation of a medicine for treating reproductive organ injury caused by diabetes mellitus, and relates to the technical field of biological medicine. Experiments prove that as an artificially synthesized indole-3-acetic acid derivative, MA-5 provides a brand new treatment thought for testis injury caused by diabetes mellitus, not only is beneficial to improvement of reproductive function, but also possibly provides a new strategy for overall treatment of diabetes mellitus. Besides, as a natural plant hormone, MA-5 has relatively high safety and acceptability, and possibly becomes a new alternative therapy with a clinical application prospect. Although the long-term administration of the MA-5 does not significantly influence the blood glucose level, the alleviation effect of the MA-5 on testis injury shows that the MA-5 as a treatment means for long-term use can continuously improve reproductive dysfunction caused by diabetes, and has potential clinical advantages.

The invention relates to the technical field of conditioners, in particular to a conditioner for improving crop stress resistance and a preparation method thereof, and the conditioner comprises the following raw materials: plant hormones, trehalose, humic acid, a complex microbial inoculant, modified nano sodium alginate, modified nano zinc oxide, lactic acid, a green tea extract and a licorice extract. The modified nano-sodium alginate, the modified nano-zinc oxide and the lactic acid play a role through a synergistic effect, the modified nano-sodium alginate and nutrient substances form a modified nano-sodium alginate gel compound through physical adsorption and chemical effects, stress-resistant peptides on the surface of the compound directly activate immune systems of crops, synthesis of stress-resistant proteins is promoted, and the stress resistance of the crops is improved. The modified nano-zinc oxide enhances the absorption of nutrient substances by crop root cells, assists the activation of crop immune systems, and synergistically enhances the disease resistance and stress resistance of crops, and the lactic acid effectively stabilizes the pH value of the conditioner, relieves the oxidation pressure of crops under adverse conditions, and enhances the physiological stability of crops.

The invention provides a deuterium-labeled 3-indoleacrylic acid isotope compound and a preparation method thereof, and relates to the technical field of plant hormone detection, deuterated toluene is creatively nitrated and subjected to a ring closing reaction, then an aldehyde group is introduced, and finally hydrolysis is performed to obtain the target product deuterium-labeled 3-indoleacrylic acid isotope compound. The preparation method provided by the invention is strong in operability, relatively good in atom economy and excellent in reproducibility and stability, the isotope abundance of the prepared deuterium-labeled 3-indoleacrylic acid isotope compound can reach 98% or above, the chemical purity is 98% or above, and the deuterium-labeled 3-indoleacrylic acid isotope compound can be used as an internal standard substance for detecting plant hormones and has good application prospects. The method has important practical application value.

The invention discloses a compound fermentation inoculant for strengthening traditional Chinese medicine residue composting efficiency and a traditional Chinese medicine residue fermentation method.The compound fermentation inoculant comprises a composting function group, a nutrient production function group and a nutrient solution, in the traditional Chinese medicine residue fermentation process, the composting function group and the nutrient production function group act synergistically, the respective characteristics are exerted in different fermentation periods, and the effect of improving the traditional Chinese medicine residue composting efficiency is achieved. The degradation of lignocellulose, the fixation of nitrogen, the synthesis of flavonol and the generation of plant hormones are respectively accelerated, so that the efficiency of the composting process and the composting quality are comprehensively improved, and the planting quality of crops and the like is favorably improved.

The invention discloses a non-tissue-culture-dependent tomato genetic transformation method, and belongs to the technical field of genetic engineering. According to the method, seedlings germinated for about one week of tomatoes are utilized, after true leaves are removed, agrobacterium carrying target gene plasmids are used for infecting wounds, then plant hormones are sprayed for induction to form calluses, then the calluses are differentiated to form a large number of seedlings, and the seedlings are subjected to seedling culture. The method comprises the following steps: carrying out preliminary screening on seedlings by using an antibiotic solution corresponding to a resistance gene on a plasmid carried by agrobacterium, and finally carrying out PCR amplification target gene identification on tomato plants subjected to preliminary screening so as to determine finally transformed positive plants. According to the method, the callus tissue is directly induced to differentiate and regenerate on the living plant, so that the step of tissue in-vitro culture required by the traditional tomato genetic transformation is simplified, the transgenic plant can be simply, conveniently and efficiently obtained, and powerful technical support is provided for tomato molecular breeding, target gene function analysis and excellent germplasm innovation.

The invention discloses a cultivation method for improving quality and aroma of flue-cured tobaccos. The cultivation method comprises the steps that 1, the flue-cured tobaccos are planted in rainy tobacco areas in the south; 2, preparing a foliar fertilizer; the leaf fertilizer is prepared from the following components in parts by mass: 1000 parts of tobacco stem leaching liquor, 100 to 500 parts of amino acid raw powder, 50 to 100 parts of potassium nitrate, 50 to 100 parts of monopotassium phosphate, 0.005 to 0.05 part of gibberellin and 100 to 250 parts of glucose; the foliar fertilizer is prepared by adding the components in proportion and fully and uniformly mixing the components. 3, before and after topping of flue-cured tobacco plants, the prepared foliar fertilizer is used for at least one time of foliar spraying operation on fresh tobacco leaves of flue-cured tobacco. According to the quality-improving and aroma-enhancing cultivation method for the flue-cured tobacco, nitrogen, phosphorus and potassium nutrient elements and plant hormones, namely gibberellin and glucose are reasonably added into the leaf fertilizer, so that sufficient growth, natural maturity and chemical component coordination regulation of the tobacco leaves are realized, and the aroma quality of the flue-cured tobacco is improved.

The invention provides an algae fermentation culture method, which relates to the technical field of bioengineering, and comprises the following steps: selecting microalgae seeds; constructing an index fed-batch model corresponding to the selected microalgae seeds; inoculating the microalgae seeds into a fermentation tank of a Basal culture medium; culturing according to the index fed-batch model under a dark condition; after culturing under a dark condition, adding plant hormones and a nitrogen source to continuously culture to obtain the microalgae. According to the algae fermentation culture method provided by the invention, the growth of microalgae and the accumulation of xanthophyll are facilitated through index fed-batch fermentation, and after the index fed-batch fermentation culture, IAA high-nitrogen combined treatment is matched, so that the xanthophyll yield and yield of the microalgae in a fermentation tank can be effectively improved.

The invention provides a method for regulating and controlling aroma of grape fruits through an exogenous plant growth regulator, which comprises the following steps of: spraying three exogenous plant hormones, namely methyl jasmonate, phenylalanine and strigolactones, on 'Hanxiangmi' grape fruits with strong rose aroma, and analyzing the aroma composition and content of the treated grape fruits. The result shows that the rose fragrance intensity of the'Hanxiangmi 'grapes is enhanced after the jasmonic acid methyl ester treatment; after strigolactones treatment, components such as alpha-terpilenol, beta-ionone, nonanoic acid, geraniol and the like in grape fruits are prominent, so that the treated grapes are converted into mixed fragrance type grapes with flower fragrance, fruit fragrance and grass fragrance, and the fragrance layers are rich; and the treatment effect of phenylalanine is not obvious. The invention provides a reference for the research of strigolactone in fruit quality.

The invention relates to the technical field of daily chemicals, in particular to an anti-hair-loss composition containing hexapeptide-11 and application of the anti-hair-loss composition. The composition comprises a foaming agent, a suspending agent, a hair conditioner, a foaming agent, a thickening agent, an emollient, a preservative, a PH regulator, a chelating agent, hexapeptide-11 and a solvent. The hexapeptide-11 can promote metabolism and proliferation of hair papilla cells; the ginseng and black bean hydrolysate provides plant hormone-like components; the radix scutellariae extract effectively inhibits scalp inflammation, and the three components have a remarkable synergistic effect in the aspects of regulating and controlling the hair follicle cycle and prolonging the growth period. Through synergistic fermentation conversion of probiotics, the molecular structure and skin absorption capacity of original components are improved, meanwhile, various active substances such as small molecular peptides, organic acids and B vitamins are released, and the scalp microenvironment can be improved. The hexapeptide-11 is not obviously degraded in the fermentation process, and forms a composite stable system together with the plant extract, so that the hexapeptide-11 has good pH adaptability and storage stability, and is suitable for daily scalp care.

The invention relates to the technical field of biology, and discloses application of a cucumber CsTOR gene in cucumber grafted seedlings, and the cucumber CsTOR gene has high expression level in tender tissues of root tips, stem tips and male flower buds of cucumbers; subcells of the cucumber CsTOR gene are localized in a cell nucleus and a cell membrane; the cucumber CsTOR gene is used for regulating and controlling the survival rate of cucumber grafted seedlings. The cucumber-pumpkin grafted seedlings treated by a control group and the TOR inhibitor are further subjected to transcriptome and metabolome analysis, and it is found that after the TOR kinase activity is inhibited, the content of plant hormone metabolism related genes and hormones is remarkably changed, especially auxin. The CsTOR-mediated auxin-regulated grafting interface healing mechanism research is preliminarily disclosed, and while the research on the heterologous grafting interface healing mechanism is enriched, theoretical support is provided for creating a rapid healing material and optimizing a grafted cucumber production technology in the future.

The present invention discloses a method for promoting the expression of selenoprotein genes in Chlamydomonas reinhardtii and increasing the yield of selenoproteins. S1. Inoculate Chlamydomonas reinhardtii algae with liquid culture medium at 20-30°C and 0-200 μmol / (m 2 S2, taking the Chlamydomonas reinhardtii culture solution, adding sodium selenite and abscisic acid, the abscisic acid concentration in the culture solution is 10-1000 μmol / L, at 20-30°C, 0-200 μmol / (m 2 ·s) Oscillating culture under light conditions. The present invention adds the plant hormone ABA during the selenium-enriched culture of Chlamydomonas reinhardtii, which not only does not affect the growth and biomass accumulation of microalgae, but also promotes the expression of selenoproteins. It is applied in the production of Chlamydomonas reinhardtii algae powder rich in selenoproteins, and can greatly increase the yield of selenoproteins.

The invention discloses a high-concentration multi-element slow-release blended fertilizer which is prepared from nutrient elements, plant hormones, ammonium bicarbonate, ammonium phosphate, potassium chloride, magnesium oxide, sub-volcanic rock, kaolin, urea, bentonite, calcium oxide, compound microorganism bacteria, diammonium hydrogen phosphate, potassium sulfate, crop waste residues, a fermentation accelerant and animal waste. The fertilizer comprises the following components in parts by weight: 2-5 parts of nutrient elements; 1-3 parts of plant hormones; and 10-15 parts of ammonium bicarbonate. The organic fertilizer and the inorganic fertilizer are combined, the product is green and environment-friendly, the utilization rate of the fertilizer is increased, the fertilizer efficiency is more remarkable, the effects of further activating soil nutrients and fertilizing soil can be achieved, root growth and cell division can be promoted, crop disease resistance can be improved, conversion and absorption of nutrient elements such as nitrogen, phosphorus and potassium in the fertilizer can be accelerated, and the fertilizer efficiency is improved. And moreover, the negative influence of the fertilizer on the environment can be effectively reduced after long-term use of the fertilizer, so that the fertilizer has good social and economic benefits.

The invention discloses a method for detecting natural plant hormones and exogenous growth regulators of vegetables, efficient separation and accurate detection of the natural plant hormones and the exogenous growth regulators are realized through combination of hydrophilic interaction chromatography and mass spectrometry, a hydrophilic interaction chromatographic column is adopted, and conditions such as mobile phase composition and flow velocity are optimized; the selectivity of polar substances is improved by utilizing hydrophilic interaction, so that compounds with similar structures achieve baseline separation, and chromatographic peak overlapping interference is avoided; the mass spectrometric detection only responds to a specific ion signal by screening characteristic parent ion / daughter ion pairs of a target object, so that matrix impurities and non-target ion interference are effectively eliminated, the false positive risk is remarkably reduced, and the detection sensitivity and specificity are improved; the combined technology is combined with the efficient separation capacity of the hydrophilic interaction chromatographic column and a multi-reaction monitoring mode, synchronous separation of multiple compounds with similar polarities in single analysis is achieved, parallel detection of dozens of target objects is supported, and the analysis time is greatly shortened.

The invention discloses saccharomycetes G-1 and application thereof in degradation of gibberellin GA3, the Latin name of the saccharomycetes G-1 is Yarrowia lipolytica (Yarrowia lipolytica), the strain of the saccharomycetes G-1 is preserved in the China General Microbiological Culture Collection Center on December 1, 2024, and the preservation number is CGMCC No.32800. The invention further discloses a preparation method of the saccharomycetes G-1. The yeast G-1 is screened from a gibberellic acid fermentation filter residue organic fertilizer sample, the strain can grow in an inorganic salt culture medium with gibberellin GA3 as a unique carbon source, and the degradation efficiency of GA3 reaches 24% after the strain is cultured for 48 h; the microbial agent prepared from the microbial agent is applied to the composting process of fermentation filter residues, and the result shows that degradation of GA3 is obviously accelerated by adding 3% of the microbial agent to a treatment group. The strain has great application potential in the aspect of promoting harmless treatment of fermentation filter residues. The invention provides a new way for removing residual plant hormone gibberellin GA3 in soil and organic fertilizers, enriches a germplasm resource library of plant hormone degrading bacteria, and has a better application prospect.

The invention discloses a thick-stem high-yield caulis spatholobi germplasm screening and breeding method suitable for an under-forest habitat. According to the method, a standardized under-forest cultivation system is constructed, spiral winding induction synergistically mediated by plant morphology and plant hormones is implemented, lignification development of stems is enhanced by applying mild mechanical stress with specific parameters, rhizosphere microorganism regulation and systematic nutrition management are synchronously combined, and the yield of the stems is increased. And finally, a multi-dimensional comprehensive evaluation system covering the stem shape, the wood density, the active ingredient content and the under-forest weak light adaptability is established, so that an integrated technical scheme for efficiently and accurately breeding the excellent caulis spatholobi germplasm with thick stem phenotype, high flavone accumulation and strong under-forest weak light tolerance from a large number of candidate germplasm is realized.

The invention discloses a method for preparing a soil conditioner for gardenia cultivation by using immobilized compound bacteria, which comprises the following steps: extracting gardenia lignin by using gardenia waste as a raw material, and preparing an inner-layer wall material to embed a mixed bacteria solution to obtain lignin inner-layer microcapsules; preparing an outer-layer wall material through modified chitosan, and compounding and curing the inner-layer microcapsule, the hydrogenated streptomyces liquid and the outer-layer wall material to obtain the immobilized composite microbial agent. And finally, mixing the immobilized complex microbial inoculant with matrixes such as leaf mold and peat soil to obtain the modifier. Ordered release of compound bacteria is realized through dual response, functional synergy of Rafissonia, Ochrobactrum and Streptomyces hydrogenii can be enhanced through population signal molecule modification, soil nutrients can be activated, effective iron can be chelated to solve the problem of iron deficiency yellowing of cape jasmine, soil pollutants and pesticide residues are degraded, and the soil quality is improved. Pathogenic bacteria reproduction is inhibited, and rhizosphere micro-ecological balance is maintained; plant hormones can be secreted to promote the growth of the cape jasmine, and the cape jasmine is converted into more components with high medicinal value.

The invention relates to the technical field of agricultural cultivation, in particular to a breeding method of water horn seeds. The method comprises the following steps: storing dried water chestnut seeds at 8-16 DEG C for 8-24 days, and soaking the water chestnut seeds in a potassium permanganate aqueous solution for 15-25 minutes; and sowing the soaked water horns seeds in a matrix, and culturing for 18-22 days to obtain seed seedlings. According to the method provided by the invention, the germination rate of the water pod seeds is extremely high and reaches 99%; and the method is short in operation period and simple in operation steps, does not need any plant hormones and chemical substances, effectively solves the breeding problem of the water hormones, and has important significance for reducing the endangered degree of the water hormones.

The invention relates to the technical field of sugarcane smut resistance, and provides a molecular marking method for sugarcane smut resistance, which comprises a sample preparation unit, a double-sugarcane area inoculation identification unit, a whole genome association analysis unit, an SNP (Single Nucleotide Polymorphism) marker development unit, a marker verification unit and a result application unit, the output end of the sample preparation unit is connected with the input end of the double-sugarcane-area inoculation identification unit through a sample transmission channel, the double-sugarcane-area inoculation identification unit outputs phenotypic data to the whole-genome association analysis unit, and the whole-genome association analysis unit outputs remarkably associated SNP sites to the SNP marker development unit; and the SNP marker development unit outputs a specific primer to the marker verification unit. The molecular marker provided by the invention is based on the correlation between the candidate gene of the site and the known disease-resistant pathway of the plant, and the MYB transcription factor can improve or reduce the resistance level of the plant to pathogenic bacteria by regulating the synthesis of secondary metabolites, transduction of plant hormone signals, expression of defensive genes and the like.

The present application belongs to the technical field of microorganisms, and relates to a Pseudomonas jessenii JY2025 and application thereof. Pseudomonas piscicola The biological preservation number of the Pseudomonas jessenii (P. jessenii) JY2025 is CGMCC No. 37283. The strain provided by the present application has broad-spectrum antifungal activity, can significantly inhibit the growth of various plant pathogenic fungi, and has the abilities of salt and alkali tolerance, inorganic phosphorus degradation, protease secretion, siderophore secretion and indole acetic acid production, which are helpful to promote crop growth and improve soil fertility.

The invention discloses a method for analyzing strigolactones hormone and receptor protein interaction based on a biomass spectrum and a Tycho instrument, and relates to the technical field of biology, the method comprises the following steps: S1, co-incubating a receptor protein solution and a strigolactones compound solution or a blank solvent to respectively obtain a to-be-detected incubation solution and a blank incubation solution; s2, respectively detecting the incubation liquid to be detected and the blank incubation liquid through a Tycho instrument to obtain a thermal denaturation curve of the incubation liquid to be detected and a thermal denaturation curve of the blank incubation liquid; s3, respectively analyzing the incubation liquid to be detected and the blank incubation liquid by using a biomass spectrum to obtain a mass spectrum of the incubation liquid to be detected and a mass spectrum of the blank incubation liquid. And judging whether the strigolactones hormone interacts with the receptor protein or not and whether the hydrolysis reaction occurs or not by comparing the difference between the peak offset of the thermal denaturation curve and the protein signal delta m / z in the mass spectrum. The method not only can be used for interaction detection of plant hormones and receptors, but also can be used for target protein excavation of natural active small molecules.

A lysogenic phage community, its extraction method and application. The tangential flow technology is used to proportionally isolate bacteria and phage communities from in-situ soil, and single-bacteria communities and single-phage communities are obtained through enrichment and concentration. At the same time, mitomycin C is added to the single-bacteria community for lysogenic induction, and then the tangential flow system is used again for filtration and enrichment to obtain a high-throughput lysogenic phage community. Finally, we obtain single-bacteria communities, bacteria-phage mixed communities, and bacteria-lysogenic phage mixed communities. The phage transplantation technology can promote the participation of indigenous microorganisms in the process of promoting plant growth and development. The transplanted lysogenic phages can participate in the synthesis and metabolism of host plant hormones by optimizing the microbial network structure and injecting auxiliary metabolic genes. This method provides a long-term and environmentally friendly microbial synergistic growth promotion technology for the development of green agriculture in China.

The invention relates to the field of agricultural biochemical engineering and plant protection, and particularly discloses a liquid film-forming agent for preventing crops from freezing injury due to late spring coldness and a preparation method of the liquid film-forming agent. The film-forming agent is prepared from carboxymethyl chitosan, sodium carboxymethyl cellulose, kasugamycin, brassinolide, glycol and glycerol mixed dihydric alcohol, Tween 80 and deionized water according to a specific proportion. The preparation method comprises the key processes of step-by-step dissolution, mixing, functional component addition and the like. Organic fusion of physical barrier, ice nucleus inactivation and physiological regulation is realized through multiple action mechanisms of natural polymer composite film forming, biopesticide targeted sterilization, plant hormone induced cold resistance and dihydric alcohol synergistic antifreezing. The product can provide efficient protection at a low temperature of-4 DEG C, significantly reduces the frost damage rate, has the characteristic of 28-day natural rapid degradation, and solves the technical bottlenecks of single function, difficult degradation and poor environmental compatibility of a traditional film agent.

The invention belongs to the technical field of plant breeding, and discloses a breeding method for improving the mutagenesis efficiency of stem tips of woody plants. The CRISPR-Cas9 ribonucleoprotein complex and plant hormones 6-benzyladenine, gibberellin and jasmonic acid are co-encapsulated and then accurately delivered to stem tip meristem of woody ornamental plants such as acer truncatum, pteroceltis tatarinowii, crape myrtle and tassel, and the gene mutation obtaining rate of the stem tip meristem is effectively increased; the intelligent environment control system is used for dynamically optimizing illumination, temperature, humidity and oxidative stress, activating a plant endogenous DNA repair pathway and further enhancing homologous recombination efficiency, meanwhile, through collaborative optimization of induction hormone signals and environmental stress, the stress resistance is improved, the leaf color, the plant type and the growth vigor can be accurately regulated and controlled, and the yield of the plant is increased. According to the method, efficient, accurate and stress-resistant linked woody plant stem tip mutation breeding is realized.