53 results about "Strigolactone" patented technology

The invention provides a method for regulating and controlling aroma of grape fruits through an exogenous plant growth regulator, which comprises the following steps of: spraying three exogenous plant hormones, namely methyl jasmonate, phenylalanine and strigolactones, on 'Hanxiangmi' grape fruits with strong rose aroma, and analyzing the aroma composition and content of the treated grape fruits. The result shows that the rose fragrance intensity of the'Hanxiangmi 'grapes is enhanced after the jasmonic acid methyl ester treatment; after strigolactones treatment, components such as alpha-terpilenol, beta-ionone, nonanoic acid, geraniol and the like in grape fruits are prominent, so that the treated grapes are converted into mixed fragrance type grapes with flower fragrance, fruit fragrance and grass fragrance, and the fragrance layers are rich; and the treatment effect of phenylalanine is not obvious. The invention provides a reference for the research of strigolactone in fruit quality.

The invention discloses a method for analyzing strigolactones hormone and receptor protein interaction based on a biomass spectrum and a Tycho instrument, and relates to the technical field of biology, the method comprises the following steps: S1, co-incubating a receptor protein solution and a strigolactones compound solution or a blank solvent to respectively obtain a to-be-detected incubation solution and a blank incubation solution; s2, respectively detecting the incubation liquid to be detected and the blank incubation liquid through a Tycho instrument to obtain a thermal denaturation curve of the incubation liquid to be detected and a thermal denaturation curve of the blank incubation liquid; s3, respectively analyzing the incubation liquid to be detected and the blank incubation liquid by using a biomass spectrum to obtain a mass spectrum of the incubation liquid to be detected and a mass spectrum of the blank incubation liquid. And judging whether the strigolactones hormone interacts with the receptor protein or not and whether the hydrolysis reaction occurs or not by comparing the difference between the peak offset of the thermal denaturation curve and the protein signal delta m / z in the mass spectrum. The method not only can be used for interaction detection of plant hormones and receptors, but also can be used for target protein excavation of natural active small molecules.

The present invention relates to the use of compounds of formula (I), which are strigolactone derivatives and analogues, as nitrification inhibiting compounds, i.e. for reducing nitrification, in nitrifying microorganisms, as well as agrochemical mixtures and compositions comprising a nitrification inhibiting compound of formula (I).

The invention relates to a regulator for inhibiting growth of wheat high-node tiller buds and application thereof in the technical field of agriculture, and the regulator for inhibiting growth of wheat high-node tiller buds comprises strigolactones artificial analogue GR24 and other nutrient elements and auxiliary preparations allowed by wheat tiller buds. The strain is used for inhibiting and regulating wheat tiller buds in a five-leaf stage or a seven-leaf stage according to a specific concentration ratio, the population size can be regulated, and more optimized environment and nutrition conditions are provided for plant growth, so that effective tiller growth and ear formation are ensured, and a new thought is provided for wheat high-yield cultivation.

The invention relates to the technical field of plant cultivation, and provides a sedge stress-resistant cultivation method and application of strigolactone derivatives in improvement of saline-alkaline resistance of sedge. According to the method, a plant growth regulator is used for carrying out exogenous spraying treatment on the sedge, the plant growth regulator comprises a strigolactone derivative, and the strigolactone derivative is selected from the following compounds shown in the specification. The cultivation method provided by the invention is efficient, environment-friendly and easy to operate, and can effectively relieve the damage of compound saline-alkaline stress to the sedge and improve the growth adaptability of the sedge in a saline-alkaline environment.

The invention provides a compound with a plant growth regulation effect as well as a preparation method and application thereof. The radix angelicae alkaloids G and H are separated and identified from the traditional Chinese medicine radix angelicae for the first time; the structure is novel, and the parent nucleus structure contains butenolactone structures which are the same as those in plant signal molecule child strigolactone, trimethyl butenolactone and the like; however, the natural contents of the two angelica dahurica alkaloids are extremely low, so that the angelica dahurica alkaloids G and H are synthesized by utilizing a chemical method, and a derivative is obtained. Researches find that the angelica dahurica alkaloid and the derivative thereof have a remarkable promoting effect on germination of dicotyledonous crop seeds and monocotyledonous crop seeds under low concentration, and have an inhibiting effect under high concentration. The angelica dahurica alkaloid and the derivative thereof can be a class of new plant hormone-like molecules, and can play a role in regulating and controlling the growth of plants.

The invention relates to the technical field of plant stress resistance regulation and control and the field of plant genetic engineering, in particular to application of strigolactones GR24 or induced protein CsJIP-like thereof in improving cold resistance of tea trees, a strigolactones GR24 solution is sprayed to the tea trees, the relative conductivity of the tea trees and the content of malondialdehyde, hydrogen peroxide and superoxide anions can be reduced, and the cold resistance of the tea trees can be improved. The activity of superoxide dismutase, peroxidase, catalase and ascorbic acid peroxidase in the tea trees is improved, the oxidative stress of the tea trees is relieved, the integrity of cell membranes is protected, cell osmotic equilibrium is maintained, and dehydration damage caused by low temperature is avoided; screening a cold-resistant gene CsJIP-like responding to GR24, and verifying that the cold-resistant gene CsJIP-like has a cold-resistant function by utilizing instantaneous overexpression and instantaneous silencing of tea tree leaves; the GR24 is high in chemical stability, small in application amount, environmentally friendly and beneficial to promoting the genetic improvement process of cold resistance of the tea trees.

The invention relates to the technical field of chemical analysis, in particular to a method for detecting endogenous strigolactones in cotton root exudates. The method comprises the following steps: (1) soaking cotton seeds in water, germinating in a dark place, transferring the cotton seeds into a culture container which is filled with a culture medium and is provided with an empty bottom, culturing the cotton seeds by using a half Hoagland's nutrient solution, culturing the cotton seeds by using a half phosphorus-deficient Hoagland's nutrient solution, and finally collecting cotton root exudates; (2) extracting cotton strigolactones from the cotton root exudates collected in the step (1) by adopting an SPE C18 column; and (3) firstly carrying out liquid chromatography separation on the cotton strigolactones obtained in the step (2), and then carrying out mass spectrometry detection. The method can realize high-content collection of endogenous strigolactones of cotton, and has the advantages of high detection accuracy, high sensitivity, simplicity, convenience, rapidness and good selectivity.

The invention belongs to the technical field of plant stress resistance, and particularly relates to a method for relieving aluminum stress of water spinach. The invention discloses a method for relieving aluminum stress of water spinach. A relieving agent is sprayed on leaves of the water spinach; the mitigating agent is composed of a spermidine trihydrochloride solution and a strigolactone solution, the concentration of the spermidine trihydrochloride solution is 0.1-0.2 mmol / L, and the concentration of the strigolactone solution is 0.1-0.5 [mu] mol / L. The spermidine trihydrochloride solution and the strigolactone solution are combined for use, inhibition of aluminum stress on elongation and growth of water spinach root systems is relieved, meanwhile, aluminum absorption of the root systems can be reduced, the SOD activity of the root systems can be enhanced, then the influence of aluminum stress on growth of the water spinach can be effectively relieved, the aluminum resistance of the water spinach is improved, and the yield of the water spinach is increased. A support can be provided for developing a medicament for relieving the aluminum stress of the vegetables or improving the aluminum resistance of the vegetables.

The present invention provides a propagation matrix and a propagation method for promoting spore production in arbuscular mycorrhizal fungi, comprising strigolactones and naphthaleneacetic acid. The present invention utilizes the synergistic effect of strigolactones and naphthaleneacetic acid, uses earthworm castings and peat as primary raw materials, and combines vermiculite, indoleacetic acid, humic acid, and calcium magnesium phosphate to prepare the arbuscular mycorrhizal fungi propagation matrix. The matrix effectively increases the root infection rate of arbuscular mycorrhizal fungi and the number of arbuscular mycorrhizal fungi spores produced. The arbuscular mycorrhizal fungi propagation matrix provided by the present invention is low-cost, simple to operate, and capable of large-scale production and application of arbuscular mycorrhizal fungi inoculants.

The present invention relates to the use of the overexpression of a Populus tomentosa strigolactone receptor PtoD14 gene in increasing a wood yield. By means of cloning a Populus tomentosa strigolactone receptor PtoD14 gene from wild-type Populus tomentosa and introducing the PtoD14 gene into Populus tomentosa plants by means of a genetic engineering method, a Populus tomentosa line with enhanced secondary development is obtained. The number of secondary xylem cell layers in 3-month-old PtoD14 transgenic Populus tomentosa is 8 to 21 layers more than that in 3-month-old non-transgenic common Populus tomentosa, which is of important significance for providing a high wood yield for the large-scale production of Populus tomentosa.

This invention discloses 5-hydroxy-3-methylfuran-2(5H)-one derivatives, their preparation methods, and applications. This invention provides three strigolactone analogs 1a-1y, 2a-2i, and 3a-3t, which exhibit excellent activity in promoting the seed germination of *Broomrapechameleon* and *Striga asiatica*. Compared with existing synthetic strigolactone analogs, they possess advantages such as simple structure, high activity, and high stability. Furthermore, the synthesis method is simple and economical, making them a class of strigolactone analogs with significant research value. They can be used for the control of root-parasitic weeds *Broomrapechameleon* and *Striga asiatica*.

The invention discloses preparation and application of a protein composite imprinting material for targeted recognition of strigolactones. The method comprises the following steps: 1, fusing halogenated alkane dehalogenase into ShHTL7 for induced expression to obtain recombinant protein; 2, preparing nano magnetic ferroferric oxide hollow microspheres; 3, carrying out surface amination modification on the nano magnetic ferroferric oxide hollow microspheres; 4, functionalizing halogenated alkanoic acid on the surfaces of the nano magnetic ferroferric oxide hollow microspheres subjected to amination modification; and 5, preparing the strigolactones surface protein molecularly imprinted polymer. The protein composite imprinting material which is high in adsorption efficiency, high in adsorption selectivity and capable of being rapidly enriched is prepared, shows superparamagnetism, can be rapidly separated under the action of a magnet, shows ultrahigh targeted recognition capacity on strigolactones, can be used for targeted extraction of strigolactones in parasitic plant root exudates, and has a good application prospect. The materials are convenient and fast to recycle and can be repeatedly used.

The invention discloses a preparation method and application of an inclusion compound for improving stability of strigolactones. The method comprises the following steps: step 1, preparing a GR24 inclusion compound solution: dissolving host molecules in deionized water, preparing a GR24 solution, then slowly dropwise adding the GR24 solution into a host molecule aqueous solution, and stirring to react for a certain time, so as to obtain the GR24 inclusion compound solution; step 2, preparation of the GR24 clathrate compound: filtering the obtained GR24 clathrate compound solution by using a hydrophilic filter membrane, then performing rotary evaporation and concentration on the mixed solution by using a rotary evaporator, and finally freeze-drying the concentrated solution to obtain the GR24 clathrate compound. The solubility of the clathrate compound prepared by the invention in a water phase is remarkably improved, and the clathrate compound has relatively high dissolution rate. In addition, according to the clathrate compound prepared by the invention, the stability of strigolactones in acid, alkali and thermal environments is remarkably improved, and the bioavailability of strigolactones is favorably improved.

The present invention relates to a strain of Trichoderma pseudokoningii Tpk20, deposited under DSM number 34838 at Leibniz-lnstitut DSMZ - German Collection of Microorganisms and Cell Cultures, which has a biostimulant action on plants, due to the specific production of compounds that increase plant nutrients availability and / or activate plant metabolic defense pathways, and which responds to strigolactone mimics by amplifying the production of compounds involved in the plant biostimulant effects.

The invention discloses an artificial cultivation method for improving the flavone content of morchella. The method comprises the following steps: producing seeds by using a morchella engineering strain; furrows are built according to cultivation technical specifications; sowing is conducted on the built furrows; performing spawn running management according to technical specifications; soaking buckwheat grains in warm water, uniformly mixing the buckwheat grains with crushed ginkgo leaves, wood chips and rice husks, adding water to a specified water content, bagging, performing high-temperature steam sterilization, and cooling for later use; placing exogenous nutrition bags: placing the prepared exogenous nutrition bags; before and after morchella primordium appears, a strigolactones (GR24) aqueous solution is sprayed on the ground according to the spraying amount; and harvesting the morchella according to technical specifications. According to the method disclosed by the invention, the total flavonoid content of the morchella esculenta product is remarkably improved by using the morchella esculenta engineering strain capable of producing flavonoids at high yield and plant hormones capable of promoting the morchella esculenta to produce flavonoids at high yield, and replacing wheat grains in a nutrition bag with buckwheat grains with high flavonoid content, so that the medicinal value of the morchella esculenta product is enhanced.

The present invention relates to the fields of chemistry and agricultural technology, and more particularly to a fluorescent molecule that inhibits strigolactone signals, its synthesis, and application. The fluorescent molecule that inhibits strigolactone signals has a structural formula as shown in Formula I. The fluorescent molecule that inhibits strigolactone signals of the present invention has a simple structure, is easy to synthesize, and has high biological activity. It can inhibit strigolactone signals and, as a new plant growth regulator, can also be used as a fluorescent molecule in basic botanical research to indicate the transport, distribution, and accumulation of strigolactones. #imgabs0# Formula I.

The invention relates to the technical field of organic synthesis, in particular to a method for asymmetrically synthesizing strigolactone derivatives GR24 through an enzyme-chemical method. The preparation method comprises the following steps: mixing alpha-tetralone with anhydrous tetrahydrofuran, and adding a lithium diisopropylamide solution for mixing reaction; 2-methyl bromoacetate and 1, 3-dimethyl-2-imidazolidinone are added for a reaction, and S3 is obtained; mixing the S3, NAD < + >, isopropanol and a buffer solution, and reacting by using a novel enzyme catalyst to obtain S4; mixing and reacting the HCO2Et solution in S4 with potassium tert-butoxide to obtain a crude product; the DMF solution of the crude product, potassium carbonate and the DMF solution of 5-bromo-3-methylfuran-2 (5H)-ketone are subjected to a reaction, and GR24 is obtained. The creativity of the method is mainly reflected in the application of the novel enzyme catalyst, and by using the novel enzyme catalyst, the reaction efficiency and selectivity are improved, the cost is reduced, the environmental pollution is reduced, and the application prospect is wide.

The invention discloses an application of pyrazolate serving as a strigolactone analogue. The pyrazolate provided by the scheme of the invention can be used as the strigolactones analogue for regulating the length of plant hypocotyl, regulating the germination of plant seeds, regulating the length and / or density of plant root hair and regulating the plant height, and has a similar effect with a strigolactones analogue rac-GR24; and pyrazol is low in price, can be applied to various plants, is small in environmental pollution, and can be applied to agricultural production on a large scale.

The present application discloses a method for analyzing the interaction between strigolactone hormones and receptor proteins based on mass spectrometry and Tycho instrument, which relates to the field of biotechnology and includes the following steps: Step S1: Incubate the receptor protein solution with the strigolactone compound solution or the blank solvent to obtain the test incubation solution and the blank incubation solution respectively; Step S2: Detect the test incubation solution and the blank incubation solution respectively by the Tycho instrument to obtain the thermal denaturation curve of the test incubation solution and the thermal denaturation curve of the blank incubation solution; Step S3: Analyze the test incubation solution and the blank incubation solution respectively using mass spectrometry to obtain the mass spectrometry of the test incubation solution and the mass spectrometry of the blank incubation solution. By comparing the peak shift of the thermal denaturation curve and the difference in protein signals in the mass spectrometry Δ m / z to determine whether the strigolactone hormone interacts with the receptor protein and whether a hydrolysis reaction occurs. This method can not only be used for the detection of the interaction between plant hormones and receptors, but also for the discovery of target proteins of natural active small molecules.

The invention discloses application of strigolactones analogues in resistance of tea trees to anthracnose pathogen infection, and relates to the technical field of agriculture. According to the application disclosed by the invention, the fac-GR24 is subjected to exogenous spraying in advance, so that not only can endogenous strigolactones signals of the tea trees be activated, but also the expression of a disease-resistant response gene PR1 can be activated, so that the oxidation damage caused by pathogenic bacterium invasion can be relieved, the disease resistance of the tea trees can be improved, and finally, the reproduction and expansion of Colletotrichum camelliae pathogenic bacteria in leaves of the tea trees can be inhibited. Experimental results show that the number of pathogenic bacteria on leaves subjected to exogenous spraying of the rac-GR24 is only 54.8% of that of a control group, the area of disease spots in the leaves is only 45.8% of that of the control group, and the obvious level is achieved. The method has great significance in the field of tea tree planting.

The application discloses a method for improving waterlogging resistance of Clematis, and comprises the following steps: 1, the cultivation substrate of Clematis potted seedlings adopts peat: coconut bran: perlite: light stone in a ratio of 55:15:15:15; 2, a mixed solution of exogenous growth regulators is configured, the mixed solution comprises 50-100 muM / L mmol / L of melatonin, 1-3 mM / L of Na2SiO3.9H2O2 and 10-30 muM / L of strigolactone, or the mixed solution comprises 1-2 muM / L of brassinolide and 1-3 mM / L of Na2SiO3.9H2O2; 3, the prepared mixed solution of exogenous growth regulators is uniformly sprayed on the upper and lower surfaces of leaves. When Clematis is subjected to waterlogging stress, the method can help to enhance the waterlogging resistance of Clematis, improve the stress resistance of Clematis, promote the growth of Clematis, and lay a foundation for promoting Clematis waterlogging resistance germplasm innovation and new variety breeding.

The application discloses application of pyrazole anthrone in strigolactone analogues. The pyrazole anthrone in the scheme can be used as a strigolactone analogue to regulate the length of a hypocotyl of a plant, promote the expression of a key inhibitor of a strigolactone signal transduction pathway SMXL2 , SMXL6 , SMXL7 , SMXL8 , increase the length and quantity of root hairs, and regulate the growth and development of the plant, and has similar effects to the strigolactone analogue pyrazole anthrone; the pyrazole anthrone is low in price, can be applied to various plants, has little environmental pollution, and can be applied on a large scale in agricultural production.

The application discloses application of a strigolactone inhibitor in prevention and treatment of TMV viruses and relates to the technical field of molecular biology. The application inoculates TMV after treating Nicotiana attenuata with a strigolactone (SL) hormone, performs transcriptome sequencing, analyzes gene conditions of difference changes, and performs qPCR verification on differentially expressed genes; research on SL-induced promotion of Nicotiana attenuata to TMV infection is carried out, and it is clear that the SL has the ability to inhibit tobacco resistance to tobacco mosaic virus (TMV); it is determined that the SL is a plant hormone for inhibiting plant defense reaction, and the SL inhibitor Tis108 has the ability to enhance tobacco resistance to TMV, and it is determined that the Tis108 is a plant hormone inhibitor for promoting plant defense reaction. The application lays a foundation for further research and utilization of the SL hormone, development of a new type of plant elicitor, safe and efficient prevention and treatment of plant diseases.

This invention discloses the application of pyrazolite as a strigolactone analog. The pyrazolite of this invention can be used as a strigolactone analog to regulate hypocotyl length, seed germination, root hair length and / or density, and plant height, exhibiting similar effects to the strigolactone analog rac-GR24. Furthermore, pyrazolite is inexpensive, applicable to a variety of plants, and has minimal environmental impact, allowing for large-scale application in agricultural production.

This invention provides strigolactone via VvORR9 The methods and applications of gene regulation of grape berry shape belong to the fields of plant molecular biology and agricultural biotechnology. This study involves regulating grape berry shape through exogenous application of the synthetic strigolactone analog GR24. VvORR9 Gene expression levels alter the longitudinal and transverse diameters of the fruit, as well as the fruit shape index. This invention, combining correlation analysis, stable transformation in grapes, and heterologous transformation in tomatoes, constructs a molecular regulatory network for strigolactone-mediated grape fruit shape regulation. It is the first to demonstrate that the strigolactone signaling pathway participates in grape fruit morphogenesis and identifies regulatory factors that respond to strigolactone. VvORR9 This provides a theoretical basis for the precise regulation of grape fruit shape and molecular breeding.

The application provides a long-acting liquid organic fertilizer capable of enhancing salt and alkali tolerance of crops and a preparation method and application thereof, and belongs to the technical field of crop planting. The long-acting liquid organic fertilizer takes fermented tail liquid of citric acid, inorganic chemical fertilizer, micro-fertilizer and strigolactone as raw materials, and comprises the following effective components: organic matter of 220 g / L or more, N of 100 g / L or more, P2O5 of 60 g / L or more, K2O of 40 g / L or more, boron and zinc of 1 g / L or more, and strigolactone of 25 micromoles / L. The raw material components in the long-acting liquid organic fertilizer cooperate with each other and have synergistic effect, and jointly play the roles of promoting crop growth, increasing biomass, improving chlorophyll content, improving nutrient utilization rate and salt and alkali tolerance. In addition, the application is also of great significance in solving environmental pollution, recycling waste liquid, reducing fertilizer production cost and developing green and healthy agriculture.

The application provides a method for improving drought resistance of endangered plant pen container tree, which mainly studies drought resistance characteristics of pen container trees with different seedling ages, uses plant growth regulators and manages after drought stress to improve drought resistance of the endangered plant pen container tree and promote its field return. The technical scheme of the application mainly applies uniform foliar spraying of exogenous growth regulators, the exogenous growth regulators are a mixed solution of melatonin, Na2SiO3.9H2O2 and strigolactone, the spraying is continuously performed before drought stress, and meanwhile, drought stress and recovery irrigation management are combined, and Na2SiO3.9H2O2 and / or melatonin are continuously sprayed to achieve the purpose of improving drought resistance of the endangered plant pen container tree.

The invention discloses a growth regulation composition for relieving continuous cropping obstacles of cucumbers and application of the growth regulation composition, and belongs to the technical field of plant growth regulation. The growth regulation composition is prepared from a chelating dopamine solution and a strigolactones solution, the chelated dopamine solution is a double-ligand chelated dopamine solution, and the preparation method comprises the following steps: uniformly mixing a zinc ion solution and an ethylenediamine tetraacetic acid salt solution, and then adding a dopamine solution, thereby obtaining the zinc ion / ethylenediamine tetraacetic acid complex. The striga lactone is dissolved in acetone, and deionized water is added for dilution to obtain a striga lactone solution. A 50 [mu] mol.L <-1 > chelated dopamine solution is applied to roots and a 1.0 [mu] mol.L <-1 > strigolactones solution is sprayed to leaf surfaces for cooperative treatment, so that the absorption rate of cucumbers to moisture and nutrient substances in soil can be remarkably improved, dry substances can be accumulated, and the tolerance of cucumber bodies to a continuous cropping mode is enhanced. The composition is small in dosage, low in cost and suitable for green prevention and control of continuous cropping obstacles of greenhouse cucumbers.

The use of a C-terminal motif of a rice D3 protein and a biomaterial thereof in regulating rice tillering. The problem to be solved is to provide a polypeptide for regulating the tiller number of rice in an OsD3 gene. In order to solve the technical problem, the provided use comprises the use of a CTH motif of the rice D3 protein in regulating the tiller number of rice, or preparing a product for regulating the tiller number of rice, or / and in rice breeding. The CTH motif of D3 is very important for the interaction between D3 and D14 in rice. Deletion of the CTH motif by using CRISPR genome editing technology can increase the tiller number of rice. The overexpression of the CTH motif of D3 can fine-tune the perception of strigolactone signaling in rice, moderately increase the tiller number of rice, and thus has a certain potential for increasing yield.