81 results about "Strigolactone" patented technology

The invention discloses a method for inhibiting growth and development of tomato side branches. The method comprises the following steps of performing exogenous coating to a tomato plant by adopting a Strigolactones solution with the concentration of 7.5-10.0umol*L-1; or performing exogenous injection to the tomato plant by adopting a Strigolactones solution with the concentration of 2.5-5.0umol*L-1; or soaking tomato seedling roots for 20-24 hours by adopting a Strigolactones solution with the concentration of 10-20.0umol*L-1 in 1-2 days before planting the seedlings. Exogenous coating and injection are executed at the leaf axilla of the tomato stem branch part, and the dosage thereof is 10uL / strain. According to the method, growth and development of tomato side branches can be obviously inhibited, labor consumption, and disease and pest spread are reduced, thereby improving plant growth and yield and quality; the method is simple in operation and pollution-free, and is applied to cultivation of an open field tomato and a greenhouse tomato.

The invention discloses a novel total synthesis method of strigolactones GR24 which is a plant hormone. The total synthesis method of the strigolactones GR24 comprises the following steps of: carrying out condensation, reduction, lactonization and condensation salt forming reaction on 1-indanone to synthesize a compound F; carrying out dehydrogenation reaction and bromination on 2-methyl butyrolactone to synthesize a compound H; and finally carrying out a substitution reaction on the compound F and the compound H to obtain the strigolactones GR24. According to the total synthesis method, raw materials are low in cost and easily available, the operation is simple and feasible, no strict water-free and oxygen-free operation is required, all steps are simple and feasible except the last step which needs column chromatography isolation, and the whole synthesis route is very suitable for large-scale production. Furthermore, the total synthesis method can be universally used for synthesis of various strigolactones derivatives.

The invention discloses tobacco strigolactones transport protein NtPDR6 and an interference expression vector and application thereof. The tobacco strigolactones transport protein NtPDR6 has an amino acid sequence shown in SEQ ID NO.4 or obtained through substituting, deleting or adding at least one amino acid on SEQ ID NO.4; and a nucleotide sequence for coding the protein is shown in SEQ ID NO.3 or is obtained through substituting, deleting or adding at least one nucleotide on SEQ ID NO.3. The protein has the effect of inhibiting lateral branch, axillary bud or lateral bud proliferation, the lateral branch growth of tobacco subjected to interference expression is obviously active, axillary bud or lateral bud proliferation is obvious, and the protein has significance for controlling the plant type of tobacco and improving the yield of tobacco.

Disclosed herein plant propagation materials, methods of manufacturing, formulations and uses thereof. The plant propagation materials disclosed herein may comprise a strigolactone obtained by a biosynthetic process. The plant propagation material may comprise a chemical mimic of a strigolactone. The strigolactone may be 5-deoxystrigol. Methods of manufacturing the plant propagation materials may comprise a chemical process. Alternatively, methods of manufacturing the plant propagation material may comprise a biosynthetic process. The methods may comprise use of one or more polynucleotides. The polynucleotides may encode a metabolite. The polynucleotides may comprise one or more genes encoding one or more components of a strigolactone pathway.

Disclosed herein plant propagation materials, methods of manufacturing, formulations and uses thereof. The plant propagation materials disclosed herein may comprise a strigolactone obtained by a biosynthetic process. The plant propagation material may comprise a chemical mimic of a strigolactone. The strigolactone may be 5-deoxystrigol. Methods of manufacturing the plant propagation materials may comprise a chemical process. Alternatively, methods of manufacturing the plant propagation material may comprise a biosynthetic process. The methods may comprise use of one or more polynucleotides. The polynucleotides may encode a metabolite. The polynucleotides may comprise one or more genes encoding one or more components of a strigolactone pathway.

The invention discloses a method for improving tea seedling field fungus rooting cuttage breeding effects. According to the method, AMF (arbuscular mycorrhizal fungi) efficient strain rhizophagus intraradices is applied into a cutting seedbed in advance, and strigolactones is sprayed after cutting is completed, so that the tea seedling field fungus rooting cuttage breeding effects are improved. Test results show that tea seedlings are bred by the method, the tea seedling field fungus rooting cuttage breeding effects can be effectively improved, infection rate of AMF for the tea seedlings is increased, the germination rate, the callus occurrence rate and the survival rate of the sea seedlings are increased, growth indexes such as stem height, root length and dry and fresh weight of the teaseedlings are facilitated, and the method has the advantages that the method is simple to operate, low in labor intensity and suitable for popularization and application in actual production, investment increase is less and the like.

The present invention discloses a rice tillering associated protein, the encoding gene and the use thereof. The protein is selected from the following proteins (a) and (b): (a) a protein which is composed of the amino acid sequence represented by the sequence 4 in the sequence table; (b) a protein which is associated with the plant tillering and is derivate from the protein (a) through substituting and / or deleting and / or adding one or a plurality of amino-acid residues to the amino acid sequence represented by the sequence 4 in the sequence table. The transgene complementation test proves that the protein D27 coded by the gene participates in the synthesizing of the strigolactone and regulates the plant tillering or the branch stem developing. Therefore, the genetic engineering technology can be used for regulating the tillering trait of the rice, regulating the nutrition using efficiency of the plant and effectively controlling the destructive parasitic weed. The D27 gene has important application value in the aspect of researching the beneficial fungus association and the destructive parasitic weed in the agriculture and natural ecosystem.

The invention discloses a drought-enduring seed coating agent of oilseed rape. The drought-enduring seed coating agent of the oilseed rape is prepared from the following raw materials in percentage bymass: 20.0-30.0% of ammonium polyacrylate, 6.0-10.0% of potassium acrylate, 0.05-0.5% of abscisic acid (ABA), 0.05-0.5% of strigolactone, 1.0-5.0% of chitosan, 3.0-5.0% of trehalose, 0.5-1.5% of cross-linking agent (vulcanizing agent S-80), 3.0-5.0% of sodium dodecyl sulfate, 10.0-20.0% of starch and 22.5-56.4% of distilled water. The coating agent is reasonable in formula and convenient to use and can improve the drought resistance of a whole growth period of the oilseed rape furthest; meanwhile, due to the fact that an induction factor special for the oilseed rape adversities resistance isadded into a preparation, the resistance of the oilseed rape to drought stress can be effectively induced, expression of corresponding drought resistance genes is activated, and the drought resistanceof the whole growth period of the oilseed rape is promoted; therefore, compared with ordinary seed drought-enduring coating agents, the preparation has the advantages that action is direct, the yieldimprovement effect is significant, an economic coefficient is high, the effect is durable, and the like.

The invention discloses a method for reducing the content of H2O2 and MDA in non-heading Chinese cabbages after low-temperature stress, and relates to the technical field of genetic breeding. A mixedsolution (containing 0.1% Tween 80) of brassinolide (BRs) of 0.1 [mu]mol.L<-1> and strigolactone (SL) of 0.1 [mu]mol.L<-1> is sprayed. The contrast is clear water spraying and BRs and and SL spraying.The concentrations of BRs and SL are the same. According to all treatment, spraying is respectively carried out at 8: 00 and 20: 00 two days before the low temperature, and the spraying parts are theback surfaces of leaves, and spraying is carried out until the surfaces of the leaves drip water. Spraying is performed for four times in total. The mixed solution provided by the invention can effectively improve the low-temperature stress resistance of non-heading Chinese cabbage seedlings, and provides an effective guidance thought for improving the quality and yield of the non-heading Chinesecabbages after low-temperature stress.

The invention relates to the field of medicine chemistry, and specific discloses application of a strigolactone analog in preparation of an anti inflammatory medicine. The anti-inflammatory activity of the strigolactone analog is found for the first time, and anti-inflammatory activity of two GR24 optical isomers are especially significant.

The invention discloses an application of strigolactone in alleviating cadmium toxicity of cadmium stress to plants and belongs to the field of governance of heavy metal pollution in soil in environmental protection and safety of agricultural products. The invention provides an agent for reducing cadmium accumulation in barley. The active component of the agent is strigolactone GR24, and the effective action concentration is 0.1 [mu]M. The invention also provides a method of reducing cadmium accumulation in barley in a condition of cadmium stress. The method comprises the following steps: transplanting barley seedlings cultured to a two-leaf stage to a plant growth medium with the strigolactone GR24; and culturing the seedlings till being harvested. The application finds that strigolactonecan alleviate cadmium toxicity of plants in the cadmium stress condition for the first time, and is applied to production of crops to control and reduce cadmium pollution of the crops, so that influence of heavy metal pollution of soil in safe production of quality of agricultural products is avoided.

The invention discloses a method for promoting single-needle algae accumulated grease by nitrogen deficiency combined with strigolactones. Single-needle algae is cultivated through two stages. The method for promoting the single-needle algae accumulated grease by the nitrogen deficiency combined with the strigolactones comprises the following steps that firstly glucose is used as an organic carbonsource to heterotrophically cultivate the single-needle algae to a logarithmic phase later period to collect algae cells; then autotrophy anazotic BG-11 is used as an inducing culture medium of a second stage to renewedly suspend the algae cells to be used as inducing algae liquid; 1mmol / L strigolactones (SLs) mother solutions are prepared by acetone, the strigolactones (SLs) mother solutions areadded into the inducing algae liquid to dilute the strigolactones (SLs) mother solutions to the concentration of 1[mu]mol / L, and the algae liquid is placed in a shaking table for lighting cultivating; and the algae cells cultivated to a stable phase are enriched by a centrifugal separation method, grease is extracted, and methyl esterification is carried out to prepare biodiesel. The method for promoting the single-needle algae accumulated grease by the nitrogen deficiency combined with the strigolactones is simple and practicable, can greatly shorten the cultivated time of the oil-producingsingle-needle algae, obviously increases the grease yield, and thus, greatly improves the efficiency of microalgae for producing the biodiesel.

The invention discloses a full-automatic online SPE-LC-MS / MS quantitative analysis method of an endogenous strigolactone in plant sample. The method comprises the following steps: (1) pretreating a plant sample to obtain a treated sample; (2) dissolving the treated sample obtained in the step (1) with a redissolving solvent to obtain a solving liquid; and (3) detecting the solving liquid in an online SPE-LC-MS / MS system, recording the strigolactone and the peak area corresponding to the stable isotope interior label of the strigolactone, and calculating according to an internal standard methodto obtain the content of endogenous strigolactone in a plant sample. The method can perform accurate absolute quantitative analysis on the endogenous strigolactone of plants, is simple and convenient, has good selectivity, high accuracy and high sensitivity, and has an important meaning to the physiological function and molecular action mechanism of strigolactone compounds.

The invention discloses a method for inhibiting growth and development of tomato lateral branches. The method comprises the steps of performing exogenous coating treatment on tomato plants by use of a strigolactone solution having the concentration of 7.5-10.0Mumol.L-1, or performing exogenous injection treatment on the tomato plants by use of a strigolactone solution having the concentration of 2.5-5.0Mumol.L-1, or soaking the root system of the tomato seedlings into a strigolactone solution having the concentration of 10-20Mumol.L-1 for 20-24 hours 1-2 days before seedling field planting. The exogenous coating treatment and the exogenous injection treatment both are performed on the axils of the main steam branch parts of the tomato, and the dosage is 10MuL. The method is capable of remarkably inhibiting the growth and development of the tomato lateral branches, reducing the labor consumption and the propagation of plant diseases and insect pests, promoting the growth of plants and improving the yield and the quality, has the characteristics of simple operations and no pollution, and is suitable for cultivating open field tomatoes and facility tomatoes.

The invention discloses a special synergist for beauveria bassiana biopesticide and a preparation method thereof, the special synergist is prepared from the following components in percentage by mass:0.05-0.5% parts of strigolactones, 1-5% parts of methanol, 5-15% parts of surfactant, 1-5% parts of filmogen, 0.5-5% parts of thickener, 3% parts of glucose, 0.5% parts of monopotassium phosphate, 0.2% parts of magnesium sulfate, 0.2% parts of calcium nitrate, and the balance of water. dissolving the filmogen, the thickener, glucose, monopotassium phosphate, magnesium sulfate and calcium nitratein water to prepare mixed liquor A; dissolving strigolactones in methanol and introducing the mixed liquid A at a stirring state, then adding a surfactant combination, after stirring, carrying out high-speed shearing for 30 min, then removing residue to obtain a finished product, the application method is to prepare the beauveria bassiana biopesticide into liquid pesticide to be applied accordingto the specification, then adding the product provided by the invention, applying the special synergist after mixing uniformly, the special synergist can significantly improve the prevention and control effect of the beauveria bassiana biopesticide, enhance the fast-acting property is enhanced and the period of preserving effect is extended.

The invention relates to the technical field of pesticides, in particular to a multi-effect pesticide composition, which is prepared from the following components in parts by weight: 0.001-1 parts ofbrassinolide, 1-25 parts of mepiquat chloride, 2.5-30 parts of paclobutrazol, 1-3 parts of strigolactone, 1-5 parts of a corn stigma extracting solution and 1-5 parts of liquorice root extract. The invention also relates to application of the multi-effect pesticide composition in a cotton or peanut planting process. The application can be used for solving the problems of cotton flower falling, budfalling and boll falling and promoting bud falling regeneration. The problems of vigorous growth and lodging of the overground parts of the peanuts can be effectively controlled, the maturing rate and the grain plumpness rate of the peanuts are increased, a good yield increasing effect is achieved, and the influence of residues of paclobutrazol in soil on next-stubble crops is reduced.

Compound of formula X wherein P1 is a fused-ring system comprising one 6-membered and two 5-membered rings; v indicates an S or R configuration; or individual isomers or pharmaceutically acceptable salts thereof, or mixtures thereof, in the preparation of an active agent for preventing or inhibiting cell proliferation or for inducing cell death.

The invention relates to the technical field of plant genetic engineering, and in particular relates to an ornithogalum thyrsoides dwarf multi-tiller OtDWARF53 gene and an application thereof. The nucleotide sequence of the gene is shown in SEO ID NO.1, and the amino acid sequence of a protein encoded by the gene is shown in SEO ID NO.2. The key gene DWARF53 (DWF53) for regulating strigolactones is isolated from ornithogalum thyrsoides based on the obtained invention patent of induction and tissue culture of foliar bulbiets of the ornithogalum thyrsoides and based on ornithogalum thyrsoides tertiary and secondary generation transcriptome conjoint analysis results (3+2), and a constitutive plant expression vector pHB+ OtDWARF53 is constructed, the pHB+ OtDWARF53 is transferred into tobacco,results find that transgenic tobacco plants show the following phenotys: plants have dwarf phenomena, multiple shoots are generated at stem tips, axillary bud lateral buds are formed at leaf axils, and the appearance is more plump, and the results indicate that the gene can have the functions of making the plants dwarf and promoting multi-tiller and multi-branch; and the invention specifically relates to the application of the gene in plant type regulation of regulating plant heights and promoting the development of the axillary bud lateral buds.

The seeds of parasitic plants of the genera Striga and Orobanche will only germinate after induction by a chemical signal exuded from the roots of their host. Many of these compounds have been isolated and identified from a number of different plant species and are collectively called the strigolactones. Here we show that the strigolactone germination stimulants are derived from the carotenoid pathway. This finding is used to create crop species that do not induce germination of parasitic plant seeds anymore and therefore are resistant to parasitic plants. Also provided is a method to use chemicals and mycorrhizae to inhibit germination stimulant production to control parasitic plants. Also provided are strigolactone overproducing trap and catch crops.

The invention belongs to the field of genetic engineering and transgenic breeding, and discloses a chrysanthemum strigolactone synthetase gene CmCCD8 and application thereof in changing a chrysanthemum flowering phase. The method for controlling the chrysanthemum flowering phase by virtue of a transgene CmCCD8 comprises the following steps: cloning a CmCCD8 gene in chrysanthemum morifolium 'Jinba';establishing a pBIG-CmCCD8 plant expression vector and transferring into a chrysanthemum leaf disc, and performing the subculture to primarily obtain a transgenic plant. The observation on a phenotype of the flowering phase of the transgenic plant discovers that compared with the non-transgenic plant, the transgenic plant can bloom normally under the sunlight for a long time. By transforming the endogenesis CmCCD8 gene, the flowering time of the chrysanthemum morifolium is controlled, and a novel and practical method can be provided for improving the ornamental quality of the chrysanthemum morifolium by utilizing the genetic engineering technology, so that the breeding process of the chrysanthemum biotechnology can be effectively promoted.

A pesticide composition containing the forchlorfenuron, Strigolactone and gamma-aminobutyric acid comprises the following components in percentage by weight: 0.05 to 10% of the forchlorfenuron, 0.001to 1% of the Strigolactone, 0.05 to 5% of the gamma-aminobutyric acid, 0.5 to 10% of an auxiliary agent, 10 to 40% of a solvent and the balance of water. The pesticide composition can obviously improve the average single fruit weight of kiwifruit, obviously increases the economic value of per mu yield, obviously improves the storage stability at normal temperature, and can be sprayed.

The invention discloses a paddy rice fertilizer capable of inhibiting ineffective tillering. The paddy rice fertilizer capable of inhibiting ineffective tillering is prepared from strigolactone, Na2SiO3*9H2O, diatomite, urea, potassium chloride, high water absorption resin, zinc sulfate, p-chlorophenoxyacetic acid soduim salt, abscisic acid, willow bark extract product, amino-oligosaccharin, and maleic hydrazide. In applications, a tillering inhibition agent is used for inhibiting tillering close at elongation stage; a plant growth inhibition agent and a silicon fertilizer are adopted to shorten the internode length of paddy rice basal parts, shorten plant height, improve lodging resistance and disease resistance; a nitrogenous fertilizer and a potash fertilizer are adopted as a floweringpromoting fertilizer and a flower protective fertilizer, to increase tillering spike rate and setting rate; yield increasing effect is obvious. The paddy rice fertilizer is capable of simplifying elongation-heading stage management, avoiding complex field drying operation, saving manpower, reducing elongation-heading stage management technology requirement, and improving economic benefit.

The invention provides a beauveria bassiana dispersible oil suspending agent and an efficient use method thereof. Strigolactones are added into the beauveria bassiana Bb455 dispersible oil suspending agent, and spore germination of the beauveria bassiana can be effectively promoted. A special auxiliary agent for the beauveria bassiana Bb455 can be completely compatible with beauveria bassiana Bb455 spores, has no influence on spore germination, improves the wetting performance and the adhesion performance of the beauveria bassiana Bb455 dispersible oil suspending agent on a target when the beauveria bassiana Bb455 dispersible oil suspending agent is applied, and improves the adhesion rate and the infection rate of the beauveria bassiana Bb455 on target pests, so that the pest killing speed and the prevention and treatment effect are improved.

The invention discloses isotopically labeled methyl furanone, an intermediate and a preparation method of isotopically labeled methyl furanone. The invention provides isotopically labeled methyl furanone 6. The invention further provides a preparation method of isotopically labeled methyl furanone 6, and the preparation method comprises the following steps: performing removal of a hydroxy protecting group and isomerization reaction on a compound 20 in the presence of an acid. The method provided by the invention comprises short reaction steps, labeling loci are stable, are labeled on a common D ring of a strigolactone type compound family and are successfully butted with ABC rings of strigolactone type compounds to obtain a variety of isotopically labeled strigolactone type compounds with different isotopic abundances which are more than 99% respectively, and the isotopically labeled methyl furanone is applicable to wide substances, is used as an internal source standard matter for GC-MS and LC-MS / MS analysis and has high detection sensitivity and good accuracy, thereby having broad market application prospects.

The invention relates to the technical field of planting, and particularly discloses an alkali stress treatment agent and a preparation method and application thereof. The alkali stress treatment agent is prepared from brassinolide, melatonin and strigolactones, damage of alkali stress to plants can be effectively relieved through cooperative use of the brassinolide, melatonin and strigolactones, the problems that most of existing methods for relieving damage of alkali stress to plants are achieved by improving soil, the environment is easily polluted, and soil hardening is caused are solved. The provided preparation method is simple, the prepared alkali stress treatment agent can adapt to local conditions and efficiently solve the influence of soil alkalization on plant growth, the alkali stress resistance of plants can be improved when the alkali stress treatment agent is applied to leaf surfaces and / or roots of the plants, and the alkali stress treatment agent has wide market prospects.

The invention discloses a melon water culture formula, which comprises the following steps that in the water culture formula, per 1000 kg of water contains 500 to 600 g of calcium nitrate, 400 to 500g of potassium nitrate, 250 to 350 g of magnesium sulfate, 50 to 150 g of ammonium dihydrogen phosphate, 20 to 40 g of chelate iron, 3 to 5 g of boracic acid, 1 to 3 g of manganese sulfate, 0.1 to 0.3g of zinc sulfate, 0.05 to 0.15 g of copper sulfate, 0.01 to 0.05 g of ammonium molybdate, 0.01 to 0.1 g of strigolactone, 0.01 to 0.1 g of brassinolide, 0.1 to 0.3 g of wood vinegar, 0.2 to 0.4 g ofcopper hydroxide, 0.01 to 0.1 g of iturin, 0.1 to 0.3 g of microbial agents and 0.1 to 0.5 g of chitosan. The formula is applied to melon culture, and has the advantages of high fertilizer absorptionspeed, good disease resistant performance, good growth condition, high yield and low cost.

The invention discloses an application method of a novel compound in improvement of soil acid stress resistance of plants, and relates to the technical field of novel compounds of soil acid stress resistance of plants, and the effective component of the compound is a mixed solution of BR and strigolactones. According to the novel compound for improving soil acid stress resistance of plants and thepreparation method thereof, a product with a certain concentration of BR and strigolactones as effective components is sprayed to roots of plant seedlings or to leaves of the seedlings, plant growthcan be promoted, the acid stress resistance of the plant is enhanced, the growth condition of the plant under acid toxicity stress can be obviously improved, and the acid stress resistance of the plant is obviously improved so that the problem that the yield and quality of crops are reduced due to acid stress is effectively solved.

The invention discloses to a method of screening mutants being insensitive to strigolactone. The method includes the steps of: 1) processing of wile-type arabidopsis thaliana seeds: A) weighing seeds and washing the seeds in distilled water; B) adding ethylmethane sulfonate and uniformly mixing the components in a fume cupboard; C) pouring processed ethylmethane sulfonate in NaOH and cleaning the seeds; D) placing the cleaned seeds in an agar solution and moving the seeds into soil by means of a pipette gun for growth; 2) screening of the mutants being insensitive to strigolactone: A) when the seeds are matured, harvesting the seeds and placing the seeds in a centrifugal tube, and disinfecting the seeds with a bleaching agent solution and placing the seeds in a refrigerator; B) sowing the seeds on an AT culture medium containing the strigolactone by means of the pipette gun; C) irradiating the culture dish, and placing the culture dish in a dark place so that the seeds are germinated; and D) selecting a long-root-system arabidopsis thaliana mutant single plant and transplanting the single plant in soil. The method has simple operations and short screening period, and can quickly and high-effectively screen the arabidopsis thaliana mutants being insensitive to the strigolactone.

The invention discloses a method for promoting the germination of cynomorium seeds by using sodium chloride and GR24. The method adopts a reagent composed of sodium chloride and strigolactone artificially synthesized analogue GR24 to promote the germination of Cynomorium cynomorium seeds. The ratio of the sodium chloride and the strigolactone synthetic analog GR24 can be 100-400mmol sodium chloride and 10-8-10-10mol strigolactone synthetic analog GR24. The method of the invention can quickly and efficiently break the dormancy of Cynomorium cynomorium seeds and make the germination rate reach 9-22% within 40 days, and the germination is orderly, which greatly saves the time of Cynomorium cynomorium breeding.