43 results about "Strigolactone" patented technology

The invention provides a method for regulating and controlling aroma of grape fruits through an exogenous plant growth regulator, which comprises the following steps of: spraying three exogenous plant hormones, namely methyl jasmonate, phenylalanine and strigolactones, on 'Hanxiangmi' grape fruits with strong rose aroma, and analyzing the aroma composition and content of the treated grape fruits. The result shows that the rose fragrance intensity of the'Hanxiangmi 'grapes is enhanced after the jasmonic acid methyl ester treatment; after strigolactones treatment, components such as alpha-terpilenol, beta-ionone, nonanoic acid, geraniol and the like in grape fruits are prominent, so that the treated grapes are converted into mixed fragrance type grapes with flower fragrance, fruit fragrance and grass fragrance, and the fragrance layers are rich; and the treatment effect of phenylalanine is not obvious. The invention provides a reference for the research of strigolactone in fruit quality.

The present invention relates to the use of compounds of formula (I), which are strigolactone derivatives and analogues, as nitrification inhibiting compounds, i.e. for reducing nitrification, in nitrifying microorganisms, as well as agrochemical mixtures and compositions comprising a nitrification inhibiting compound of formula (I).

The invention relates to the technical field of plant cultivation, and provides a sedge stress-resistant cultivation method and application of strigolactone derivatives in improvement of saline-alkaline resistance of sedge. According to the method, a plant growth regulator is used for carrying out exogenous spraying treatment on the sedge, the plant growth regulator comprises a strigolactone derivative, and the strigolactone derivative is selected from the following compounds shown in the specification. The cultivation method provided by the invention is efficient, environment-friendly and easy to operate, and can effectively relieve the damage of compound saline-alkaline stress to the sedge and improve the growth adaptability of the sedge in a saline-alkaline environment.

The invention relates to the technical field of plant stress resistance regulation and control and the field of plant genetic engineering, in particular to application of strigolactones GR24 or induced protein CsJIP-like thereof in improving cold resistance of tea trees, a strigolactones GR24 solution is sprayed to the tea trees, the relative conductivity of the tea trees and the content of malondialdehyde, hydrogen peroxide and superoxide anions can be reduced, and the cold resistance of the tea trees can be improved. The activity of superoxide dismutase, peroxidase, catalase and ascorbic acid peroxidase in the tea trees is improved, the oxidative stress of the tea trees is relieved, the integrity of cell membranes is protected, cell osmotic equilibrium is maintained, and dehydration damage caused by low temperature is avoided; screening a cold-resistant gene CsJIP-like responding to GR24, and verifying that the cold-resistant gene CsJIP-like has a cold-resistant function by utilizing instantaneous overexpression and instantaneous silencing of tea tree leaves; the GR24 is high in chemical stability, small in application amount, environmentally friendly and beneficial to promoting the genetic improvement process of cold resistance of the tea trees.

The invention relates to the technical field of chemical analysis, in particular to a method for detecting endogenous strigolactones in cotton root exudates. The method comprises the following steps: (1) soaking cotton seeds in water, germinating in a dark place, transferring the cotton seeds into a culture container which is filled with a culture medium and is provided with an empty bottom, culturing the cotton seeds by using a half Hoagland's nutrient solution, culturing the cotton seeds by using a half phosphorus-deficient Hoagland's nutrient solution, and finally collecting cotton root exudates; (2) extracting cotton strigolactones from the cotton root exudates collected in the step (1) by adopting an SPE C18 column; and (3) firstly carrying out liquid chromatography separation on the cotton strigolactones obtained in the step (2), and then carrying out mass spectrometry detection. The method can realize high-content collection of endogenous strigolactones of cotton, and has the advantages of high detection accuracy, high sensitivity, simplicity, convenience, rapidness and good selectivity.

The present invention provides a propagation matrix and a propagation method for promoting spore production in arbuscular mycorrhizal fungi, comprising strigolactones and naphthaleneacetic acid. The present invention utilizes the synergistic effect of strigolactones and naphthaleneacetic acid, uses earthworm castings and peat as primary raw materials, and combines vermiculite, indoleacetic acid, humic acid, and calcium magnesium phosphate to prepare the arbuscular mycorrhizal fungi propagation matrix. The matrix effectively increases the root infection rate of arbuscular mycorrhizal fungi and the number of arbuscular mycorrhizal fungi spores produced. The arbuscular mycorrhizal fungi propagation matrix provided by the present invention is low-cost, simple to operate, and capable of large-scale production and application of arbuscular mycorrhizal fungi inoculants.

This invention discloses 5-hydroxy-3-methylfuran-2(5H)-one derivatives, their preparation methods, and applications. This invention provides three strigolactone analogs 1a-1y, 2a-2i, and 3a-3t, which exhibit excellent activity in promoting the seed germination of *Broomrapechameleon* and *Striga asiatica*. Compared with existing synthetic strigolactone analogs, they possess advantages such as simple structure, high activity, and high stability. Furthermore, the synthesis method is simple and economical, making them a class of strigolactone analogs with significant research value. They can be used for the control of root-parasitic weeds *Broomrapechameleon* and *Striga asiatica*.

The invention discloses preparation and application of a protein composite imprinting material for targeted recognition of strigolactones. The method comprises the following steps: 1, fusing halogenated alkane dehalogenase into ShHTL7 for induced expression to obtain recombinant protein; 2, preparing nano magnetic ferroferric oxide hollow microspheres; 3, carrying out surface amination modification on the nano magnetic ferroferric oxide hollow microspheres; 4, functionalizing halogenated alkanoic acid on the surfaces of the nano magnetic ferroferric oxide hollow microspheres subjected to amination modification; and 5, preparing the strigolactones surface protein molecularly imprinted polymer. The protein composite imprinting material which is high in adsorption efficiency, high in adsorption selectivity and capable of being rapidly enriched is prepared, shows superparamagnetism, can be rapidly separated under the action of a magnet, shows ultrahigh targeted recognition capacity on strigolactones, can be used for targeted extraction of strigolactones in parasitic plant root exudates, and has a good application prospect. The materials are convenient and fast to recycle and can be repeatedly used.

The invention discloses a preparation method and application of an inclusion compound for improving stability of strigolactones. The method comprises the following steps: step 1, preparing a GR24 inclusion compound solution: dissolving host molecules in deionized water, preparing a GR24 solution, then slowly dropwise adding the GR24 solution into a host molecule aqueous solution, and stirring to react for a certain time, so as to obtain the GR24 inclusion compound solution; step 2, preparation of the GR24 clathrate compound: filtering the obtained GR24 clathrate compound solution by using a hydrophilic filter membrane, then performing rotary evaporation and concentration on the mixed solution by using a rotary evaporator, and finally freeze-drying the concentrated solution to obtain the GR24 clathrate compound. The solubility of the clathrate compound prepared by the invention in a water phase is remarkably improved, and the clathrate compound has relatively high dissolution rate. In addition, according to the clathrate compound prepared by the invention, the stability of strigolactones in acid, alkali and thermal environments is remarkably improved, and the bioavailability of strigolactones is favorably improved.

The present invention relates to a strain of Trichoderma pseudokoningii Tpk20, deposited under DSM number 34838 at Leibniz-lnstitut DSMZ - German Collection of Microorganisms and Cell Cultures, which has a biostimulant action on plants, due to the specific production of compounds that increase plant nutrients availability and / or activate plant metabolic defense pathways, and which responds to strigolactone mimics by amplifying the production of compounds involved in the plant biostimulant effects.

The present invention relates to the fields of chemistry and agricultural technology, and more particularly to a fluorescent molecule that inhibits strigolactone signals, its synthesis, and application. The fluorescent molecule that inhibits strigolactone signals has a structural formula as shown in Formula I. The fluorescent molecule that inhibits strigolactone signals of the present invention has a simple structure, is easy to synthesize, and has high biological activity. It can inhibit strigolactone signals and, as a new plant growth regulator, can also be used as a fluorescent molecule in basic botanical research to indicate the transport, distribution, and accumulation of strigolactones. #imgabs0# Formula I.

The invention discloses an application of pyrazolate serving as a strigolactone analogue. The pyrazolate provided by the scheme of the invention can be used as the strigolactones analogue for regulating the length of plant hypocotyl, regulating the germination of plant seeds, regulating the length and / or density of plant root hair and regulating the plant height, and has a similar effect with a strigolactones analogue rac-GR24; and pyrazol is low in price, can be applied to various plants, is small in environmental pollution, and can be applied to agricultural production on a large scale.

The invention discloses application of strigolactones analogues in resistance of tea trees to anthracnose pathogen infection, and relates to the technical field of agriculture. According to the application disclosed by the invention, the fac-GR24 is subjected to exogenous spraying in advance, so that not only can endogenous strigolactones signals of the tea trees be activated, but also the expression of a disease-resistant response gene PR1 can be activated, so that the oxidation damage caused by pathogenic bacterium invasion can be relieved, the disease resistance of the tea trees can be improved, and finally, the reproduction and expansion of Colletotrichum camelliae pathogenic bacteria in leaves of the tea trees can be inhibited. Experimental results show that the number of pathogenic bacteria on leaves subjected to exogenous spraying of the rac-GR24 is only 54.8% of that of a control group, the area of disease spots in the leaves is only 45.8% of that of the control group, and the obvious level is achieved. The method has great significance in the field of tea tree planting.

The application discloses a method for improving waterlogging resistance of Clematis, and comprises the following steps: 1, the cultivation substrate of Clematis potted seedlings adopts peat: coconut bran: perlite: light stone in a ratio of 55:15:15:15; 2, a mixed solution of exogenous growth regulators is configured, the mixed solution comprises 50-100 muM / L mmol / L of melatonin, 1-3 mM / L of Na2SiO3.9H2O2 and 10-30 muM / L of strigolactone, or the mixed solution comprises 1-2 muM / L of brassinolide and 1-3 mM / L of Na2SiO3.9H2O2; 3, the prepared mixed solution of exogenous growth regulators is uniformly sprayed on the upper and lower surfaces of leaves. When Clematis is subjected to waterlogging stress, the method can help to enhance the waterlogging resistance of Clematis, improve the stress resistance of Clematis, promote the growth of Clematis, and lay a foundation for promoting Clematis waterlogging resistance germplasm innovation and new variety breeding.

The application discloses application of pyrazole anthrone in strigolactone analogues. The pyrazole anthrone in the scheme can be used as a strigolactone analogue to regulate the length of a hypocotyl of a plant, promote the expression of a key inhibitor of a strigolactone signal transduction pathway SMXL2 , SMXL6 , SMXL7 , SMXL8 , increase the length and quantity of root hairs, and regulate the growth and development of the plant, and has similar effects to the strigolactone analogue pyrazole anthrone; the pyrazole anthrone is low in price, can be applied to various plants, has little environmental pollution, and can be applied on a large scale in agricultural production.

The application discloses application of a strigolactone inhibitor in prevention and treatment of TMV viruses and relates to the technical field of molecular biology. The application inoculates TMV after treating Nicotiana attenuata with a strigolactone (SL) hormone, performs transcriptome sequencing, analyzes gene conditions of difference changes, and performs qPCR verification on differentially expressed genes; research on SL-induced promotion of Nicotiana attenuata to TMV infection is carried out, and it is clear that the SL has the ability to inhibit tobacco resistance to tobacco mosaic virus (TMV); it is determined that the SL is a plant hormone for inhibiting plant defense reaction, and the SL inhibitor Tis108 has the ability to enhance tobacco resistance to TMV, and it is determined that the Tis108 is a plant hormone inhibitor for promoting plant defense reaction. The application lays a foundation for further research and utilization of the SL hormone, development of a new type of plant elicitor, safe and efficient prevention and treatment of plant diseases.

This invention discloses the application of pyrazolite as a strigolactone analog. The pyrazolite of this invention can be used as a strigolactone analog to regulate hypocotyl length, seed germination, root hair length and / or density, and plant height, exhibiting similar effects to the strigolactone analog rac-GR24. Furthermore, pyrazolite is inexpensive, applicable to a variety of plants, and has minimal environmental impact, allowing for large-scale application in agricultural production.

This invention provides strigolactone via VvORR9 The methods and applications of gene regulation of grape berry shape belong to the fields of plant molecular biology and agricultural biotechnology. This study involves regulating grape berry shape through exogenous application of the synthetic strigolactone analog GR24. VvORR9 Gene expression levels alter the longitudinal and transverse diameters of the fruit, as well as the fruit shape index. This invention, combining correlation analysis, stable transformation in grapes, and heterologous transformation in tomatoes, constructs a molecular regulatory network for strigolactone-mediated grape fruit shape regulation. It is the first to demonstrate that the strigolactone signaling pathway participates in grape fruit morphogenesis and identifies regulatory factors that respond to strigolactone. VvORR9 This provides a theoretical basis for the precise regulation of grape fruit shape and molecular breeding.

The application provides a long-acting liquid organic fertilizer capable of enhancing salt and alkali tolerance of crops and a preparation method and application thereof, and belongs to the technical field of crop planting. The long-acting liquid organic fertilizer takes fermented tail liquid of citric acid, inorganic chemical fertilizer, micro-fertilizer and strigolactone as raw materials, and comprises the following effective components: organic matter of 220 g / L or more, N of 100 g / L or more, P2O5 of 60 g / L or more, K2O of 40 g / L or more, boron and zinc of 1 g / L or more, and strigolactone of 25 micromoles / L. The raw material components in the long-acting liquid organic fertilizer cooperate with each other and have synergistic effect, and jointly play the roles of promoting crop growth, increasing biomass, improving chlorophyll content, improving nutrient utilization rate and salt and alkali tolerance. In addition, the application is also of great significance in solving environmental pollution, recycling waste liquid, reducing fertilizer production cost and developing green and healthy agriculture.

The application provides a method for improving drought resistance of endangered plant pen container tree, which mainly studies drought resistance characteristics of pen container trees with different seedling ages, uses plant growth regulators and manages after drought stress to improve drought resistance of the endangered plant pen container tree and promote its field return. The technical scheme of the application mainly applies uniform foliar spraying of exogenous growth regulators, the exogenous growth regulators are a mixed solution of melatonin, Na2SiO3.9H2O2 and strigolactone, the spraying is continuously performed before drought stress, and meanwhile, drought stress and recovery irrigation management are combined, and Na2SiO3.9H2O2 and / or melatonin are continuously sprayed to achieve the purpose of improving drought resistance of the endangered plant pen container tree.

The invention discloses a growth regulation composition for relieving continuous cropping obstacles of cucumbers and application of the growth regulation composition, and belongs to the technical field of plant growth regulation. The growth regulation composition is prepared from a chelating dopamine solution and a strigolactones solution, the chelated dopamine solution is a double-ligand chelated dopamine solution, and the preparation method comprises the following steps: uniformly mixing a zinc ion solution and an ethylenediamine tetraacetic acid salt solution, and then adding a dopamine solution, thereby obtaining the zinc ion / ethylenediamine tetraacetic acid complex. The striga lactone is dissolved in acetone, and deionized water is added for dilution to obtain a striga lactone solution. A 50 [mu] mol.L <-1 > chelated dopamine solution is applied to roots and a 1.0 [mu] mol.L <-1 > strigolactones solution is sprayed to leaf surfaces for cooperative treatment, so that the absorption rate of cucumbers to moisture and nutrient substances in soil can be remarkably improved, dry substances can be accumulated, and the tolerance of cucumber bodies to a continuous cropping mode is enhanced. The composition is small in dosage, low in cost and suitable for green prevention and control of continuous cropping obstacles of greenhouse cucumbers.

The use of a C-terminal motif of a rice D3 protein and a biomaterial thereof in regulating rice tillering. The problem to be solved is to provide a polypeptide for regulating the tiller number of rice in an OsD3 gene. In order to solve the technical problem, the provided use comprises the use of a CTH motif of the rice D3 protein in regulating the tiller number of rice, or preparing a product for regulating the tiller number of rice, or / and in rice breeding. The CTH motif of D3 is very important for the interaction between D3 and D14 in rice. Deletion of the CTH motif by using CRISPR genome editing technology can increase the tiller number of rice. The overexpression of the CTH motif of D3 can fine-tune the perception of strigolactone signaling in rice, moderately increase the tiller number of rice, and thus has a certain potential for increasing yield.

Aspects of the present disclosure relate to methods of cultivating genetically altered plants with increased activity of one or more of a NODULATION SIGNALING PATHWAY 1 (NSP1) protein or a NODULATION SIGNALING PATHWAY 2 (NSP2) protein that have increased mycorrhization and / or promoted symbiotic responses under high phosphate and / or high nitrate conditions. Further aspects of the present disclosure relate to methods of cultivating genetically altered plants with increased activity of a C-TERMINALLY ENCODED PEPTIDE (CEP peptide) that have increased mycorrhization and / or promoted symbiotic responses under high phosphate and / or high nitrate conditions. In addition, aspects of the present disclosure relate to methods of cultivating these plants that include exogenous application of strigolactones, karrikins, and / or CEP peptides to increase mycorrhization and / or promote symbiotic responses under specific nutrient conditions.

The application discloses a C-terminal motif of rice D3 protein and application of a biomaterial thereof in regulating rice tillering, and belongs to the technical field of biotechnology.The application comprises application of a CTH motif of the rice D3 protein in regulating the number of rice tillers or preparing a product for regulating the number of rice tillers and / or rice breeding.The application shows that the CTH motif of D3 is very important for interaction between D3 and D14 in the rice body, and the number of rice tillers can be increased by deleting the CTH motif by using a CRISPR genome editing technology.The application has the advantages that overexpression of the CTH motif of D3 can fine-tune reception of a strigolactone signal in the rice and moderately increase the number of rice tillers, and has certain yield-increasing potential.

The invention relates to the technical field of rape molecular breeding, in particular to a bioinformatics analysis method for rape hybrid combination optimization, which comprises the following steps: taking 20-50 parts of rape parent materials, extracting genomic DNA and total RNA, performing genomic sequencing and transcriptome sequencing, obtaining gene expression data, detecting DNA methylation and histone modification sites, and obtaining the rape hybrid combination. The method comprises the following steps: screening grease synthesis genes containing SNP (Single Nucleotide Polymorphism), measuring the contents of fatty acid, amino acid and the like in seeds, synchronously measuring the contents of strigolactone and jasmonic acid methyl ester, constructing a network by using WGCNA, introducing a regulatory factor relationship, screening core genes with the grease association degree of more than or equal to 0.8, constructing a random forest model based on a gene matching degree, a metabolite complementation index and the like, and outputting first 10-20 combinations. The F1 generation characters are tested through field tests, and the optimal combination is determined through repeated hybridization. According to the method, multi-dimensional data are integrated to construct a network, through intelligent model prediction, dynamic optimization and multi-character verification, the prediction accuracy and breeding efficiency are improved, and different ecotype parents are adapted.

Aspects of the present disclosure relate to methods of cultivating genetically altered plants with increased activity of one or more of a NODULATION SIGNALING PATHWAY 1 (NSP1) protein or a NODULATION SIGNALING PATHWAY 2 (NSP2) protein that have increased mycorrhization and / or promoted symbiotic responses under high phosphate and / or high nitrate conditions. Further aspects of the present disclosure relate to methods of cultivating genetically altered plants with increased activity of a C-TERMINALLY ENCODED PEPTIDE (CEP peptide) that have increased mycorrhization and / or promoted symbiotic responses under high phosphate and / or high nitrate conditions. In addition, aspects of the present disclosure relate to methods of cultivating these plants that include exogenous application of strigolactones, karrikins, and / or CEP peptides to increase mycorrhization and / or promote symbiotic responses under specific nutrient conditions.

The invention belongs to the technical field of artificial cultivation of plants, and particularly relates to a simple, convenient and efficient seedling raising method for boea cavaleriei and application thereof. The seedling culture method comprises the following steps: adding disinfected boea cavaleriei seeds and strigolactones analogue solution into a 1 / 2 MS culture medium for seed culture to obtain boea cavaleriei seedlings; the concentration of the striga lactone analogue solution is 0.1 to 10 [mu] mol / L; the volume ratio of the strigolactone analogue solution to the 1 / 2 MS culture medium is (1-3): 15; placing the black boea cavaleriei seedlings in a new 1 / 2MS culture medium, and performing seedling culture to obtain black boea cavaleriei seedlings; in the 1 / 2MS culture medium, the mass volume concentration of the cane sugar is 0.5%-2%. The seedling raising method is easy to operate and low in cost, the germination rate and the seedling rate of the black boea mellea seeds can be greatly increased, the seedling raising period is shortened, and a solid foundation is laid for breaking through the technical bottleneck of artificial cultivation and production of black boea mellea and achieving industrial development of the black boea mellea.

The application discloses a method for promoting the formation and growth of macadamia roots. The method comprises the following steps: first, cultivating the macadamia seedlings with a vegetative growth solution A, irrigating each seedling with 200-400 mL of the vegetative growth solution A every 2-3 days, and continuing for 9-10 days; second, cultivating the seedlings with a nutrient mixed solution after the cultivation with the vegetative growth solution A is completed, irrigating each seedling with 300-400 mL of the nutrient mixed solution every 2-3 days, and continuing for 25-30 days; the nutrient mixed solution comprises 40-100 micromoles per liter of naphthalene acetic acid (NAA), 10-40 micromoles per liter of strigolactone, and a vegetative growth solution B; and after the cultivation period of the nutrient mixed solution is completed, the macadamia seedlings can form abundant macadamia roots, which is beneficial to the absorption of the nutrients in the soil by the macadamia. The method combines the vegetative growth solution, the strigolactone and the naphthalene acetic acid in the growth process, promotes the root formation ability of the macadamia, and increases the formation of the macadamia roots.

This invention belongs to the field of wastewater biological treatment technology, specifically a GR24-regulated triphasic algae-bacterial symbiotic and its construction method for treating pig farm wastewater. This invention uses *Chlorella vulgaris*-endophytic bacteria S395-2-*Polyspora spiralis* as the dominant bacterial species, inoculating them into a co-culture system at a certain cell number ratio, and adding 10... ‑9 The synthetic strigolactone analog GR24 was cultured under specific temperature, light intensity, and light-dark ratio conditions to construct a triphasic algal-bacterial symbiotic with uniform spherical diameter and strong stress resistance. This triphasic algal-bacterial symbiotic was used for the pretreatment of pig farm wastewater. This invention is simple to operate, low in cost, and can significantly improve the stability of the symbiotic in high-stress wastewater, providing reliable materials for subsequent antibiotic resistance gene (ARG) reduction, making it suitable for industrial-scale promotion.

The invention belongs to the technical field of plant growth regulators, and particularly relates to a five-membered azacycloindole compound and application thereof in preparation of strigolactone inhibitors. The synthesized five-membered azacyclo-indole compound can be applied to preparation of strigolactones inhibitors, can significantly promote tillering of rice, and has an obvious inhibition effect on germination of various parasitic weed seeds induced by strigolactones, including striga formosana, Orobanche cucurbitae, Orobanche helianthi and the like. The characteristics show that the five-membered azacycloindole compound can be used as a potential plant growth regulator and has a wide prospect in agricultural application.