27 results about "Strigolactone" patented technology

The invention relates to the technical field of plant cultivation, and provides a sedge stress-resistant cultivation method and application of strigolactone derivatives in improvement of saline-alkaline resistance of sedge. According to the method, a plant growth regulator is used for carrying out exogenous spraying treatment on the sedge, the plant growth regulator comprises a strigolactone derivative, and the strigolactone derivative is selected from the following compounds shown in the specification. The cultivation method provided by the invention is efficient, environment-friendly and easy to operate, and can effectively relieve the damage of compound saline-alkaline stress to the sedge and improve the growth adaptability of the sedge in a saline-alkaline environment.

The invention discloses preparation and application of a protein composite imprinting material for targeted recognition of strigolactones. The method comprises the following steps: 1, fusing halogenated alkane dehalogenase into ShHTL7 for induced expression to obtain recombinant protein; 2, preparing nano magnetic ferroferric oxide hollow microspheres; 3, carrying out surface amination modification on the nano magnetic ferroferric oxide hollow microspheres; 4, functionalizing halogenated alkanoic acid on the surfaces of the nano magnetic ferroferric oxide hollow microspheres subjected to amination modification; and 5, preparing the strigolactones surface protein molecularly imprinted polymer. The protein composite imprinting material which is high in adsorption efficiency, high in adsorption selectivity and capable of being rapidly enriched is prepared, shows superparamagnetism, can be rapidly separated under the action of a magnet, shows ultrahigh targeted recognition capacity on strigolactones, can be used for targeted extraction of strigolactones in parasitic plant root exudates, and has a good application prospect. The materials are convenient and fast to recycle and can be repeatedly used.

The invention discloses a preparation method and application of an inclusion compound for improving stability of strigolactones. The method comprises the following steps: step 1, preparing a GR24 inclusion compound solution: dissolving host molecules in deionized water, preparing a GR24 solution, then slowly dropwise adding the GR24 solution into a host molecule aqueous solution, and stirring to react for a certain time, so as to obtain the GR24 inclusion compound solution; step 2, preparation of the GR24 clathrate compound: filtering the obtained GR24 clathrate compound solution by using a hydrophilic filter membrane, then performing rotary evaporation and concentration on the mixed solution by using a rotary evaporator, and finally freeze-drying the concentrated solution to obtain the GR24 clathrate compound. The solubility of the clathrate compound prepared by the invention in a water phase is remarkably improved, and the clathrate compound has relatively high dissolution rate. In addition, according to the clathrate compound prepared by the invention, the stability of strigolactones in acid, alkali and thermal environments is remarkably improved, and the bioavailability of strigolactones is favorably improved.

The invention discloses application of strigolactones analogues in resistance of tea trees to anthracnose pathogen infection, and relates to the technical field of agriculture. According to the application disclosed by the invention, the fac-GR24 is subjected to exogenous spraying in advance, so that not only can endogenous strigolactones signals of the tea trees be activated, but also the expression of a disease-resistant response gene PR1 can be activated, so that the oxidation damage caused by pathogenic bacterium invasion can be relieved, the disease resistance of the tea trees can be improved, and finally, the reproduction and expansion of Colletotrichum camelliae pathogenic bacteria in leaves of the tea trees can be inhibited. Experimental results show that the number of pathogenic bacteria on leaves subjected to exogenous spraying of the rac-GR24 is only 54.8% of that of a control group, the area of disease spots in the leaves is only 45.8% of that of the control group, and the obvious level is achieved. The method has great significance in the field of tea tree planting.

The application discloses a method for improving waterlogging resistance of Clematis, and comprises the following steps: 1, the cultivation substrate of Clematis potted seedlings adopts peat: coconut bran: perlite: light stone in a ratio of 55:15:15:15; 2, a mixed solution of exogenous growth regulators is configured, the mixed solution comprises 50-100 muM / L mmol / L of melatonin, 1-3 mM / L of Na2SiO3.9H2O2 and 10-30 muM / L of strigolactone, or the mixed solution comprises 1-2 muM / L of brassinolide and 1-3 mM / L of Na2SiO3.9H2O2; 3, the prepared mixed solution of exogenous growth regulators is uniformly sprayed on the upper and lower surfaces of leaves. When Clematis is subjected to waterlogging stress, the method can help to enhance the waterlogging resistance of Clematis, improve the stress resistance of Clematis, promote the growth of Clematis, and lay a foundation for promoting Clematis waterlogging resistance germplasm innovation and new variety breeding.

The application discloses application of a strigolactone inhibitor in prevention and treatment of TMV viruses and relates to the technical field of molecular biology. The application inoculates TMV after treating Nicotiana attenuata with a strigolactone (SL) hormone, performs transcriptome sequencing, analyzes gene conditions of difference changes, and performs qPCR verification on differentially expressed genes; research on SL-induced promotion of Nicotiana attenuata to TMV infection is carried out, and it is clear that the SL has the ability to inhibit tobacco resistance to tobacco mosaic virus (TMV); it is determined that the SL is a plant hormone for inhibiting plant defense reaction, and the SL inhibitor Tis108 has the ability to enhance tobacco resistance to TMV, and it is determined that the Tis108 is a plant hormone inhibitor for promoting plant defense reaction. The application lays a foundation for further research and utilization of the SL hormone, development of a new type of plant elicitor, safe and efficient prevention and treatment of plant diseases.

This invention provides strigolactone via VvORR9 The methods and applications of gene regulation of grape berry shape belong to the fields of plant molecular biology and agricultural biotechnology. This study involves regulating grape berry shape through exogenous application of the synthetic strigolactone analog GR24. VvORR9 Gene expression levels alter the longitudinal and transverse diameters of the fruit, as well as the fruit shape index. This invention, combining correlation analysis, stable transformation in grapes, and heterologous transformation in tomatoes, constructs a molecular regulatory network for strigolactone-mediated grape fruit shape regulation. It is the first to demonstrate that the strigolactone signaling pathway participates in grape fruit morphogenesis and identifies regulatory factors that respond to strigolactone. VvORR9 This provides a theoretical basis for the precise regulation of grape fruit shape and molecular breeding.

The application provides a long-acting liquid organic fertilizer capable of enhancing salt and alkali tolerance of crops and a preparation method and application thereof, and belongs to the technical field of crop planting. The long-acting liquid organic fertilizer takes fermented tail liquid of citric acid, inorganic chemical fertilizer, micro-fertilizer and strigolactone as raw materials, and comprises the following effective components: organic matter of 220 g / L or more, N of 100 g / L or more, P2O5 of 60 g / L or more, K2O of 40 g / L or more, boron and zinc of 1 g / L or more, and strigolactone of 25 micromoles / L. The raw material components in the long-acting liquid organic fertilizer cooperate with each other and have synergistic effect, and jointly play the roles of promoting crop growth, increasing biomass, improving chlorophyll content, improving nutrient utilization rate and salt and alkali tolerance. In addition, the application is also of great significance in solving environmental pollution, recycling waste liquid, reducing fertilizer production cost and developing green and healthy agriculture.

The application provides a method for improving drought resistance of endangered plant pen container tree, which mainly studies drought resistance characteristics of pen container trees with different seedling ages, uses plant growth regulators and manages after drought stress to improve drought resistance of the endangered plant pen container tree and promote its field return. The technical scheme of the application mainly applies uniform foliar spraying of exogenous growth regulators, the exogenous growth regulators are a mixed solution of melatonin, Na2SiO3.9H2O2 and strigolactone, the spraying is continuously performed before drought stress, and meanwhile, drought stress and recovery irrigation management are combined, and Na2SiO3.9H2O2 and / or melatonin are continuously sprayed to achieve the purpose of improving drought resistance of the endangered plant pen container tree.

The invention discloses a growth regulation composition for relieving continuous cropping obstacles of cucumbers and application of the growth regulation composition, and belongs to the technical field of plant growth regulation. The growth regulation composition is prepared from a chelating dopamine solution and a strigolactones solution, the chelated dopamine solution is a double-ligand chelated dopamine solution, and the preparation method comprises the following steps: uniformly mixing a zinc ion solution and an ethylenediamine tetraacetic acid salt solution, and then adding a dopamine solution, thereby obtaining the zinc ion / ethylenediamine tetraacetic acid complex. The striga lactone is dissolved in acetone, and deionized water is added for dilution to obtain a striga lactone solution. A 50 [mu] mol.L <-1 > chelated dopamine solution is applied to roots and a 1.0 [mu] mol.L <-1 > strigolactones solution is sprayed to leaf surfaces for cooperative treatment, so that the absorption rate of cucumbers to moisture and nutrient substances in soil can be remarkably improved, dry substances can be accumulated, and the tolerance of cucumber bodies to a continuous cropping mode is enhanced. The composition is small in dosage, low in cost and suitable for green prevention and control of continuous cropping obstacles of greenhouse cucumbers.

The use of a C-terminal motif of a rice D3 protein and a biomaterial thereof in regulating rice tillering. The problem to be solved is to provide a polypeptide for regulating the tiller number of rice in an OsD3 gene. In order to solve the technical problem, the provided use comprises the use of a CTH motif of the rice D3 protein in regulating the tiller number of rice, or preparing a product for regulating the tiller number of rice, or / and in rice breeding. The CTH motif of D3 is very important for the interaction between D3 and D14 in rice. Deletion of the CTH motif by using CRISPR genome editing technology can increase the tiller number of rice. The overexpression of the CTH motif of D3 can fine-tune the perception of strigolactone signaling in rice, moderately increase the tiller number of rice, and thus has a certain potential for increasing yield.

Aspects of the present disclosure relate to methods of cultivating genetically altered plants with increased activity of one or more of a NODULATION SIGNALING PATHWAY 1 (NSP1) protein or a NODULATION SIGNALING PATHWAY 2 (NSP2) protein that have increased mycorrhization and / or promoted symbiotic responses under high phosphate and / or high nitrate conditions. Further aspects of the present disclosure relate to methods of cultivating genetically altered plants with increased activity of a C-TERMINALLY ENCODED PEPTIDE (CEP peptide) that have increased mycorrhization and / or promoted symbiotic responses under high phosphate and / or high nitrate conditions. In addition, aspects of the present disclosure relate to methods of cultivating these plants that include exogenous application of strigolactones, karrikins, and / or CEP peptides to increase mycorrhization and / or promote symbiotic responses under specific nutrient conditions.

The application discloses a C-terminal motif of rice D3 protein and application of a biomaterial thereof in regulating rice tillering, and belongs to the technical field of biotechnology.The application comprises application of a CTH motif of the rice D3 protein in regulating the number of rice tillers or preparing a product for regulating the number of rice tillers and / or rice breeding.The application shows that the CTH motif of D3 is very important for interaction between D3 and D14 in the rice body, and the number of rice tillers can be increased by deleting the CTH motif by using a CRISPR genome editing technology.The application has the advantages that overexpression of the CTH motif of D3 can fine-tune reception of a strigolactone signal in the rice and moderately increase the number of rice tillers, and has certain yield-increasing potential.

The invention relates to the technical field of rape molecular breeding, in particular to a bioinformatics analysis method for rape hybrid combination optimization, which comprises the following steps: taking 20-50 parts of rape parent materials, extracting genomic DNA and total RNA, performing genomic sequencing and transcriptome sequencing, obtaining gene expression data, detecting DNA methylation and histone modification sites, and obtaining the rape hybrid combination. The method comprises the following steps: screening grease synthesis genes containing SNP (Single Nucleotide Polymorphism), measuring the contents of fatty acid, amino acid and the like in seeds, synchronously measuring the contents of strigolactone and jasmonic acid methyl ester, constructing a network by using WGCNA, introducing a regulatory factor relationship, screening core genes with the grease association degree of more than or equal to 0.8, constructing a random forest model based on a gene matching degree, a metabolite complementation index and the like, and outputting first 10-20 combinations. The F1 generation characters are tested through field tests, and the optimal combination is determined through repeated hybridization. According to the method, multi-dimensional data are integrated to construct a network, through intelligent model prediction, dynamic optimization and multi-character verification, the prediction accuracy and breeding efficiency are improved, and different ecotype parents are adapted.

Aspects of the present disclosure relate to methods of cultivating genetically altered plants with increased activity of one or more of a NODULATION SIGNALING PATHWAY 1 (NSP1) protein or a NODULATION SIGNALING PATHWAY 2 (NSP2) protein that have increased mycorrhization and / or promoted symbiotic responses under high phosphate and / or high nitrate conditions. Further aspects of the present disclosure relate to methods of cultivating genetically altered plants with increased activity of a C-TERMINALLY ENCODED PEPTIDE (CEP peptide) that have increased mycorrhization and / or promoted symbiotic responses under high phosphate and / or high nitrate conditions. In addition, aspects of the present disclosure relate to methods of cultivating these plants that include exogenous application of strigolactones, karrikins, and / or CEP peptides to increase mycorrhization and / or promote symbiotic responses under specific nutrient conditions.

The invention belongs to the technical field of artificial cultivation of plants, and particularly relates to a simple, convenient and efficient seedling raising method for boea cavaleriei and application thereof. The seedling culture method comprises the following steps: adding disinfected boea cavaleriei seeds and strigolactones analogue solution into a 1 / 2 MS culture medium for seed culture to obtain boea cavaleriei seedlings; the concentration of the striga lactone analogue solution is 0.1 to 10 [mu] mol / L; the volume ratio of the strigolactone analogue solution to the 1 / 2 MS culture medium is (1-3): 15; placing the black boea cavaleriei seedlings in a new 1 / 2MS culture medium, and performing seedling culture to obtain black boea cavaleriei seedlings; in the 1 / 2MS culture medium, the mass volume concentration of the cane sugar is 0.5%-2%. The seedling raising method is easy to operate and low in cost, the germination rate and the seedling rate of the black boea mellea seeds can be greatly increased, the seedling raising period is shortened, and a solid foundation is laid for breaking through the technical bottleneck of artificial cultivation and production of black boea mellea and achieving industrial development of the black boea mellea.

The application discloses a method for promoting the formation and growth of macadamia roots. The method comprises the following steps: first, cultivating the macadamia seedlings with a vegetative growth solution A, irrigating each seedling with 200-400 mL of the vegetative growth solution A every 2-3 days, and continuing for 9-10 days; second, cultivating the seedlings with a nutrient mixed solution after the cultivation with the vegetative growth solution A is completed, irrigating each seedling with 300-400 mL of the nutrient mixed solution every 2-3 days, and continuing for 25-30 days; the nutrient mixed solution comprises 40-100 micromoles per liter of naphthalene acetic acid (NAA), 10-40 micromoles per liter of strigolactone, and a vegetative growth solution B; and after the cultivation period of the nutrient mixed solution is completed, the macadamia seedlings can form abundant macadamia roots, which is beneficial to the absorption of the nutrients in the soil by the macadamia. The method combines the vegetative growth solution, the strigolactone and the naphthalene acetic acid in the growth process, promotes the root formation ability of the macadamia, and increases the formation of the macadamia roots.

This invention belongs to the field of wastewater biological treatment technology, specifically a GR24-regulated triphasic algae-bacterial symbiotic and its construction method for treating pig farm wastewater. This invention uses *Chlorella vulgaris*-endophytic bacteria S395-2-*Polyspora spiralis* as the dominant bacterial species, inoculating them into a co-culture system at a certain cell number ratio, and adding 10... ‑9 The synthetic strigolactone analog GR24 was cultured under specific temperature, light intensity, and light-dark ratio conditions to construct a triphasic algal-bacterial symbiotic with uniform spherical diameter and strong stress resistance. This triphasic algal-bacterial symbiotic was used for the pretreatment of pig farm wastewater. This invention is simple to operate, low in cost, and can significantly improve the stability of the symbiotic in high-stress wastewater, providing reliable materials for subsequent antibiotic resistance gene (ARG) reduction, making it suitable for industrial-scale promotion.

The invention belongs to the technical field of plant growth regulators, and particularly relates to a five-membered azacycloindole compound and application thereof in preparation of strigolactone inhibitors. The synthesized five-membered azacyclo-indole compound can be applied to preparation of strigolactones inhibitors, can significantly promote tillering of rice, and has an obvious inhibition effect on germination of various parasitic weed seeds induced by strigolactones, including striga formosana, Orobanche cucurbitae, Orobanche helianthi and the like. The characteristics show that the five-membered azacycloindole compound can be used as a potential plant growth regulator and has a wide prospect in agricultural application.

The application belongs to the technical field of plant genetic engineering, and particularly relates to application of GhDWARF27 gene in regulation of plant verticillium wilt resistance and breeding of verticillium wilt resistant plants. The application discloses that application of GR24, an artificial synthetic analogue of strigolactone (SLs), or overexpression of a SLs biosynthesis gene GhDWARF27 can improve the resistance of plants to verticillium wilt, and silencing of the GhDWARF27 gene can result in reduction of SLs content in roots of the plants and reduction of the resistance of the plants to verticillium wilt. SLs can activate expression of JA response related genes through JA and ABA biosynthesis and signal pathways, and can enhance the resistance of the plants to verticillium wilt in an ABA dependent manner by enhancing the activity of antioxidant enzymes. The technical scheme and molecular mechanism of SLs for enhancing the resistance of cotton to verticillium wilt will provide a new approach for cotton breeding and prevention of cotton verticillium wilt.

Disclosed herein are compounds and methods for regulating plant growth and / or combating root parasitic plants. Formulations containing one or more disclosed compounds or salts thereof, and one or more excipients are disclosed. Methods for regulating plant growth typically includes applying one or more formulations to a plant, a plant part, or a growing site of plant, where the one or more compounds or salts thereof can be in an effective amount to inhibit tillering, trigger senescence, and / or cause stunted growth of the plant. The plant can be a cereal, grain, or vegetable plant. Methods for combating root parasitic plants typically includes applying one or more formulations to a root parasitic plant, a plant part of root parasitic plant, or a growing site of root parasitic plant, where the one or more compounds or salts thereof can be in an effective amount to induce parasitic seed germination.

The present application relates to the field of plant stress resistance regulation and the field of plant genetic engineering, and particularly relates to application of a strigolactone GR24 or an induced protein CsJIP-like in improving cold resistance of a tea plant, spraying a strigolactone GR24 solution on the tea plant can reduce relative conductivity, contents of malondialdehyde, hydrogen peroxide and superoxide anion of the tea plant, improve activities of superoxide dismutase, peroxidase, catalase and ascorbate peroxidase of the tea plant, reduce oxidative stress of the tea plant, protect cell membrane integrity, maintain cell osmotic balance, and avoid dehydration damage caused by low temperature; a cold-resistant gene responding to the GR24 is screened CsJIP-like , and the tea plant leaf is used for verifying the cold-resistant function by transient overexpression and transient silencing; the GR24 has high chemical stability, needs small application amount, and is environment-friendly, and is helpful to promote the genetic improvement process of the tea plant cold resistance.

The application provides a method for regulating the aroma of grape fruits by an exogenous plant growth regulator, and utilizes methyl jasmonate, phenylalanine and strigolactone to spray 'Hanxiangmi' grape fruits with rich rose fragrance, and analyzes the aroma composition and content of the treated grape fruits. The results show that the rose fragrance intensity of 'Hanxiangmi' grape is enhanced after methyl jasmonate treatment; after strigolactone treatment, the components such as alpha-terpineol, beta-ionone, nonanoic acid and geraniol in the grape fruits are prominent, so that the treated grape is changed into mixed type grape with flower fragrance, fruit fragrance and grass fragrance, and the aroma is rich in layers; and the treatment effect of phenylalanine is not obvious. The application provides a reference for the research of strigolactone on fruit quality.

The invention discloses a strigolactones-based formula for promoting symbiosis of arbuscular mycorrhizal fungi, and relates to the technical field of agricultural biology.The strigolactones-based formula comprises an active composition composed of the following components and an agriculturally acceptable carrier, the strigolactones signal substance comprises ent-5-deoxystrigool stereoisomers with biological activity, and the content of the ent-5-deoxystrigool stereoisomers accounts for more than 80% of the total mass of the signal substance; the biological surface active agent is one or more of rhamnolipid, sophorolipid and tea saponin; according to the strigolactones-based formula for promoting symbiosis of arbuscular mycorrhizal fungi, the lowest content (gt; gt) of high-activity ent-5-deoxystrigolactones stereoisomers in strigolactones signal substances is limited; 80%) of the core signal molecules, and a specific type of biosurfactant (rhamnolipid, sophorolipid or tea saponin) is cooperatively used, so that the transmission efficiency and the interface contact effect of the core signal molecules are improved.

The application discloses a reagent, a method and application for reducing the astringency of grape fruits, wherein the reagent comprises strigolactone, brassinolide and melatonin. It is found that strigolactone can significantly reduce the astringency of grape fruits at a concentration of 1-3 μmol / L, with a maximum reduction of 50.59%, and does not affect the coloring of the fruits; low-concentration brassinolide (0.1-0.6 mg / L) can reduce the astringency while increasing the contents of total phenols and anthocyanins, and synergistically promote the coloring; and melatonin has astringency-reducing effect at a concentration of 5-50 μmol / L. Transcriptome analysis shows that strigolactone blocks tannin synthesis by inhibiting the expression of DFR and CCR genes, and brassinolide remodels the metabolic flow to the anthocyanin branch through BZR1. The application provides an efficient and safe technical means for the precise improvement of the flavor quality of table grapes.

The invention discloses an application of strigolactone in promoting symbiosis of arbuscular mycorrhizal fungi, and relates to the field of agricultural microorganisms, and an effective amount of strigolactone or a derivative thereof is combined with at least one arbuscular mycorrhizal fungi propagule for use; wherein the use concentration of the striga asiatica lactone is 0.01 nM to 10 M; according to the application of strigolactone in promoting symbiosis of arbuscular mycorrhizal fungi, through a Tween-20-containing solution carrier and a combined application mode, the activity of strigolactone is effectively protected, the action time of strigolactone is prolonged, the defect that strigolactone is easy to degrade is overcome, and the stability and repeatability of a field application effect are guaranteed.

The invention provides a preservation method of dwarf bananas. According to the method, strigolactones analogue GR24 is combined with spontaneous controlled atmosphere treatment, so that the preservation time of dwarf bananas is prolonged. The specific optimal operation comprises the following steps: selecting dwarf bananas which are uniform in size, proper in fruit plumpness, consistent in fruit size, free of mechanical damage and free of plant diseases and insect pests, performing sterilization treatment, soaking the dwarf bananas in GR24 with a proper concentration for a proper time, performing air drying, and performing complete sealing by using a polyethylene anti-fog bag so as to form a storage space subjected to modified atmosphere treatment; and storing and transporting under a normal temperature or refrigeration condition. According to the invention, yellowing and softening of dwarf bananas can be obviously delayed, the refreshing time and shelf life of dwarf bananas are obviously prolonged, the oxidation resistance of fruits is improved, and the quality of the fruits is maintained. The preservation method disclosed by the invention has the characteristics of good preservation effect and simplicity and convenience in operation, is safe, low-carbon and environment-friendly, and can be widely applied to storage and transportation of dwarf bananas under normal temperature or refrigeration conditions.