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Method for reducing content of H2O2 and MDA in non-heading Chinese cabbages after low-temperature stress

A headed cabbage, H2O2 technology, which is applied in the directions of botanical equipment and methods, gardening methods, gardening tools/equipment, etc., can solve the problems of weak resistance to low temperature stress of non-heading cabbage, and achieves increased low temperature stress resistance, realization of The effect of protection and environmental pollution-free

Pending Publication Date: 2021-02-26
ANHUI AGRICULTURAL UNIVERSITY +1
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to overcome the deficiencies of the prior art, and provides a method for reducing H in non-heading Chinese cabbage after low temperature stress. 2 o 2 and the method of MDA content, to solve the technical problem that non-heading cabbage resistance to low temperature stress is weak in the prior art

Method used

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  • Method for reducing content of H2O2 and MDA in non-heading Chinese cabbages after low-temperature stress
  • Method for reducing content of H2O2 and MDA in non-heading Chinese cabbages after low-temperature stress
  • Method for reducing content of H2O2 and MDA in non-heading Chinese cabbages after low-temperature stress

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Effect of mixed solution on non-heading Chinese cabbage seedlings under low temperature 2 o 2 and the effect of MDA content

[0027] According to Solebol "Hydrogen peroxide H 2 o 2 ) content detection kit "instructions for the determination of H in the sample 2 o 2 content. The content of MDA in the samples was determined by the thiobarbituric acid method. Depend on figure 1 It can be seen that after low temperature, non-heading Chinese cabbage seedling leaves H 2 o 2 The contents of MDA and MDA increased significantly, and the contents of both sprayed with EBR or SL alone would decrease to varying degrees, while the contents of the two in the leaves of plants sprayed with EBR and SL decreased significantly, and there was little difference between them and normal temperature.

[0028] At the same time, hydrogen peroxide (H 2 o 2 ) is a kind of active oxygen, which can cause oxidative damage to plant cells, and the increase of its content will increase membran...

Embodiment 2

[0032] Effects of Mixture on T-AOC and RWC Contents of Non-heading Chinese Cabbage Seedlings at Low Temperature

[0033] The determination of total antioxidant capacity refers to the total antioxidant level composed of various antioxidant substances and antioxidant enzymes in the sample. After 5 days of low temperature treatment, the total antioxidant capacity (T-AOC) detection kit was used by Suleibao. The collected plant leaves were assayed. The relative water content of plant leaves is an important indicator of the water status of plant tissues, and it is of special significance for the selection of resistance to reverse breeding; it is measured by dry and fresh weight of leaves. Its test results are as figure 2 , the total antioxidant capacity (T-AOC) and relative water content (RWC) of non-heading cabbage seedling leaves were significantly reduced, and the contents of both spraying EBR or SL alone would increase to varying degrees, while the mixed spraying of EBR and T...

Embodiment 3

[0035] Effects of Mixture on Photosynthetic Mechanism of Non-heading Chinese Cabbage Seedlings at Low Temperature

[0036] After 5 days of low temperature treatment, the fluorescence emitted by chlorophyll in the plant leaves after 30min dark adaptation was measured with a continuous excitation fluorometer plant efficiency instrument (PEA, Hansatech, UK). The detection results are as follows: image 3 .

[0037] V j It can reflect the degree of closure of active reaction centers when illuminated for 2ms. P.I. abs It is the photosynthetic performance index, which can reflect the state of the photosynthetic mechanism of the plant. The parameter is sensitive and can well reflect the influence of stress on the photosynthetic mechanism. As can be seen from the figure above, after low temperature, PI abs The sharp decrease indicates that the low temperature has an impact on the photosynthetic apparatus. After spraying EBR or SL alone, there is a slight increase, but it is much d...

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Abstract

The invention discloses a method for reducing the content of H2O2 and MDA in non-heading Chinese cabbages after low-temperature stress, and relates to the technical field of genetic breeding. A mixedsolution (containing 0.1% Tween 80) of brassinolide (BRs) of 0.1 [mu]mol.L<-1> and strigolactone (SL) of 0.1 [mu]mol.L<-1> is sprayed. The contrast is clear water spraying and BRs and and SL spraying.The concentrations of BRs and SL are the same. According to all treatment, spraying is respectively carried out at 8: 00 and 20: 00 two days before the low temperature, and the spraying parts are theback surfaces of leaves, and spraying is carried out until the surfaces of the leaves drip water. Spraying is performed for four times in total. The mixed solution provided by the invention can effectively improve the low-temperature stress resistance of non-heading Chinese cabbage seedlings, and provides an effective guidance thought for improving the quality and yield of the non-heading Chinesecabbages after low-temperature stress.

Description

technical field [0001] The invention relates to the technical field of vegetable seedling cultivation, in particular to a method for alleviating low temperature stress damage to non-heading Chinese cabbage seedlings. Background technique [0002] Non-heading Chinese cabbage is a variety of Brassicaceae Brassica subspecies. It is an important autumn and winter vegetable and is cultivated in most parts of my country, especially in the Yangtze and Huaihe River basins. Non-heading cabbage is beautiful in appearance, rich in multivitamins, and has high nutritional value. Therefore, it is deeply loved by everyone and has become a popular vegetable. [0003] At present, low temperature stress is the main environmental factor that seriously affects plant growth and crop yield. After non-heading Chinese cabbage plants are subjected to low temperature stress, their growth will be significantly inhibited, and the fresh weight and dry weight of the aboveground parts of the plant will de...

Claims

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Application Information

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IPC IPC(8): A01G22/15A01G7/06A01N43/22A01N43/08A01P21/00
CPCA01G22/15A01G7/06A01N43/22A01N43/08
Inventor 汪承刚赵梦茹袁凌云朱世东王洁侯金锋黄兴学唐小燕张泽根陈国户
Owner ANHUI AGRICULTURAL UNIVERSITY
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