401results about How to "Reduce MDA content" patented technology

The present invention relates to a production method of feed additive containing selenium-enriched probiotics and its product. Said production method includes the following steps: using torula yeast and acidophilous lactobacillus as strain, mixing them according to the ratio of 1:4 and inoculating them into culture containing sodium selenite, adding organic selenium transformation accelerator and making culture for 36 hr so as to obtain the invented product. In said product the CFU numbers of torula yeast and acidophilous lactobacillus respectively are 10 to the power 9 / g and 10 to the power 11 / g, selenium content is 50 mg / kg, in which organic selenium content is above 90%, and selenomethionine content is above 75%. It can obviously raise animal immunological function and resistance to disease.

The invention discloses a cultivation method for improving corn yield in saline-alkali land. The method comprises the following steps: irrigating the saline-alkali land for flooding for once in the middle of March, wherein the irrigation volume for per mu is 60-100 m<3>, airing for 1-1.5 months until the surface of earth is dry enough to allow people to step on, applying 1000kg-1500kg of miscellaneous manure or 50kg-55kg of compound fertilizer per mu, then performing rotary tillage for 1-2 times to mix soil and the fertilizer, pressing by using a harrow and preserving soil moisture, and leveling the surface of earth; treating corn by using a seed coating agent before the corn is seeded, wherein the seed coating agent comprises actinomycin, bacillus mucilaginosus, azospirillum, diatomite, spicy powder, heteroauxin, potassium sulphate, magnesium sulfate, zinc sulfate, an alpha-pimacol growth regulator, an EDTA-2Na chelating agent, humic acid and turf; mixing bactericide and insecticide with seeds for treating the seeds, namely, firstly adding the insecticide, and then adding the bactericide after piling for 3-6 hours; and immediately seeding after the seed mixing treatment is finished.

The present invention relates to biological processing of garlic, and is especially biological processing method of odorless garlic. The biological processing method of odorless garlic includes the steps of washing, soaking, fermenting and drying, and features that under certain temperature and humidity conditions, the enzyme inside garlic is activated through natural fermentation, so as to eliminate bad smell, improve taste and raise the antioxidant property of garlic. Animal test proves the maintained or even strengthened physiological functions of the deodorized garlic.

The invention discloses an anti-oxidant composition for improving animal immune function and a preparation method thereof, belonging to the field of feed additive. The composition comprises the following ingredients: 2.0-18.0 wt% of vitamin E, 5.0-30.0 wt% of vitamin C, 2.0-40.0 wt% of thioctic acid, 1.0-25.0 wt% of antioxidants, and the balance consisting of corncob powder, wherein, the antioxidants are mixtures of one or more of daidzein, astragalus polysaccharides and tea polyphenol. According to basic theory of free radical biology, various antioxidants are used to form an antioxidant network, because of the synergism among the antioxidants, the oxidation resistance of the animal that takes the composition is enhanced greatly, so that the immune tissue and organs of the organism are protected, and the animal immune function is improved.

Provided is a pig forage improving meat quality. The pig forage is characterized in that the pig forage is composed of the following ingredients, by weight, 30 parts of corn flour, 20 parts of puffed corn, 15 parts of sorghum, 15 parts of soybean meal, 15 parts of wheat flour, 10 parts of wheat bran, 0.1 part of choline chloride, 0.1 part of methionine, 0.1 part of threonine, 0.5 part of lysine, 0.2 part of salt, 1 part of Chinese herbal medicine materials, 0.15 part of microecologics, 0.15 part of vitamin premix, 0.3 part of trace element premix and 0.02 part of compound enzyme preparation. Based on the safe and relieved principles, trace elements, vitamins and functional substances are blended with energy raw materials, protein raw materials, amino acids and the like according to certain ratios, and a pig mixed forage promoting growth, lowering material consumption, raising the lean meat percentage and improving meat quality is developed.

The invention discloses a seedling formation method in alkaline lands by pre-coating film on cotton, which aims at increasing the seedling formation rate of the cotton in alkaline lands and realizing seedlings full and strong. The method of the invention is characterized in that: cotton fields having the salt content of below 0.4% and has certain accumulative rain and snow in winter are thawed in early spring, then ditches are opened in the rows of the pre-sowed cotton, and 2 tons of miscellaneous manure and 40 to 60kg of compound fertilizer which contains about 15 percent of N, about 15 percent of P2O5 and about 15 percent of K2O are fertilized, and then mulching film is used for covering the ditches immediately after the ditches are covered with soil and raked; as for an alkaline land with salt content of more than 0.4 percent, 80m<3> of fresh water is irrigated for each mu (mu: a Chinese unit of area, 1 / 15 of a hectare) at the end of winter to restrain salt content, ditches are opened and then miscellaneous manure and N-P-K compound fertilizer are fertilized in the early of middle of March, and film is covered after the land is prepared. The cotton fields which are leveled, fertilized and pre-coated with film are steadily passes earth temperature of 15 DEG C at the position of 5cm below the film, then bunch planting the ditinted and coated cotton seeds according to preset row spacing and plant spacing. The cottons are thinned out after the young seedling grow tidily, and final singling is carried out after two true leaves come out. The method of the invention achieves the functions of increasing temperature, water preserving and controlling salt by pre-coating the mulching film, promotes the seedling emergency and formation of cottons in alkaline land sand realizes synergetic salt control effect by concentrated fertilization under the film, thereby having significant yield increasing effect.

The invention discloses a plant stress resistance related protein TaWrky48 and a coding gene and application thereof. The plant stress resistance related protein provided by the invention is a protein of a), b) or c): a) protein with an amino acid sequence which is as shown by sequence 2 in a sequence table; b) fused protein which is obtained by connecting a label to the N end and / or C end of the protein with the amino acid sequence which is as shown by sequence 2 in the sequence table; c) protein which has the same functions and is obtained by substituting and / or deleting and / or adding one or a plurality of amino acid residues to the amino acid sequence which is as shown by sequence 2 in the sequence table. As proved by tests, the drought resistance of trans-TaWrky48 rice obtained by introducing the coding gene of the plant stress resistance related protein TaWrky48 disclosed by the invention to wild rice is obviously higher than that of the wild rice, and it has important theoretical and practical significance to the cultivation of new stress-resistant rice varieties, especially the cultivation of drought-resistant rice.

The invention discloses an anti-fatigue energy composition which is prepared by the following raw materials in parts by weight: expanded black rice flour, oatmeal, coconut oil, corn syrup, soybean protein isolate powder, maltodextrin, carnitine, vine tea flavone extract, grape seed extract, quercetin extract, octacosanol, caffeine, branched chain amino acid, taurine, low fat cocoa and concentrated vanilla; the anti-fatigue energy composition is capable of rapidly supplementing sugar, fat, protein, electrolyte and other nutrients which are needed by the organism and promoting the nutrients to be absorbed, can be used for rapidly supplementing the consumed carbohydrate and the lost nutrients of human body caused by sweating excessively under the condition of exercises, especially medium and high intensity exercises and exercise training, and is beneficial for relieving central and peripheral fatigue; after the anti-fatigue energy composition is adopted, the antioxidant capacity and the aerobic metabolism level of the organism are improved, the oxygen utilization rate of cells can be improved, the moment and fatigue resistance of the organism can be better rapidly improved, and the sport injury is avoided.

The invention relates to medicine and food homologous plant concentrated juice which is prepared from the following raw materials in parts by weight: 2-45 parts of hovenia dulcis thunb, 1-25 parts of kudzuvine root, 1-20 parts of dandelion, 1-16 parts of liquorice, 1-16 parts of hawthorns and 1-10 parts of pericarpium citri reticulatae. The invention also relates to a preparation method for the medicine and food homologous plant concentrated juice. The preparation method comprises the following steps of (1) concentrated juice extraction, (2) alcohol precipitation and (3) water precipitation. The medicine and food homologous plant concentrated juice disclosed by the invention has the beneficial effect of effectively alleviating alcoholic liver injury.

The invention relates to a storing and fresh preserving method spinach, pertaining to the technical field of vegetable storage and fresh preservation. The method comprises the steps of: (1) harvesting the spinach that accords with the harvesting standard in time; (2) rapidly pre-cooling the spinach at the temperature of 4 to 7 DEG C; (3) dividing and packing the spinach into special freshness preservation packages and then tying package mouths tightly and putting into a refrigeratory at the temperature of minus 1 to 2 DEG C and with the humidity of 90 to 98 percent for 2 to 3 hours, then untying the package mouths; inserting empty tubes with the inside diameter of 30mm into the packages; tying the package mouths again for maintaining ventilation degree of the package mouths and automatically regulating the gas environment in the packages with 5 to 8 percent of CO2 and 8 to 12 percent of O2; (4) taking 5 to 7 days as a circle to proceed with the management of opening the package mouths for ventilation for 2 to 3 hours and inserting the empty tubes and tying the package mouths again until the storing is finished. The method of the invention has low cost, good storing effect and is simple and convenient for operation, and the spinach can still maintain good marketability after 25 days. The method can be widely introduced to vegetable growers, dealers or processing enterprises.

The invention provides a lactobacillus-ginseng polysaccharide composition and its preparation method and use, and belongs to the technical field of lactobacillus compositions. The lactobacillus-ginseng polysaccharide composition comprises 4-6 parts of a lactobacillus plantarum C88 starter, 9-11 parts of ginseng extract, 28-32 parts of dried skim milk, 18-22 parts of cane sugar, 30-32 parts of dextrin, 1.6-2.4 parts of mannitol, 0.8-1.2 parts of magnesium stearate and 0.8-1.2 parts of citric acid. The lactobacillus-ginseng polysaccharide composition has good antioxidation performances which comprise removing DPPH free radical and ABTS free radical in vitro, improving vitality of antioxidant enzyme in naturally aging mouse serum and liver in an in-vivo test and reducing MDA content of naturally aging mouse serum and liver.

The invention relates to effervescent tablets with health care function which comprises the following constituents (by weight portions): rhodiola root extract 2-20%, edible acid 20-30%, edible alkali 25-35%, bulking agent 20-40%, binding agent 0-5%, flavoring agent 0-3%, lubricating agent 0-5%. The invention features in rapid absorption, high biological availability and sufficient curative effect.

The invention relates to a method for improving antioxidant enzyme activity of ryegrass by adopting fine domestic garbage compost under drought conditions. The method comprises the following steps of: uniformly scattering garbage trace fertilizers with grain diameters of 1,200 nanometers, 600 nanometers and 300 nanometers respectively on the surface of soil, wherein the weight part ratio of the grain diameter to the soil is 1: 60-80; then, sowing 0.5 gram of tall fescue, controlling the temperature to be between 20 and 26 DEG C and relative humidity to be between 40 and 60 percent, and illuminating the tall fescue with indoor natural light; and fully watering the tall fescue at the initial sowing stage, weighing the water in an amount which is 55 to 65 percent of the field holding amount every day or every two days after two weeks to maintain the water in a stress range, and measuring each index on the 44th day. The method provides technical support for applying the fine-grain urban domestic garbage compost to a lawn planting system.

The invention relates to a gingko diterpene lactone composition. The extract can lower the neurological deficit score of a cerebral ischemia-reperfusion rat, reduce the cerebral ischemia area and thewater content after brain tissue edema and improve the SOD activity while reducing the MDA content; moreover, the composition can inhibit rabbit platelet aggregation induced by PAF and ADP inducers atdifferent time points so as to reduce the maximum aggregation rate; furthermore, experiments indicate that the effect of each monomer compound in the gingko diterpene lactone composition is remarkably enhanced at certain proportion.

The invention relates to a method for planting lawn plants by combining rare earth cerium with mixed culture matrices. The method comprises the step of sowing seeds of lawn plants on mixed culture matrices for lawn planting, wherein 1.5-2kg of mixed culture matrix soil contains 200-800mg / Kg of rare earth cerium, and the mixed culture matrices refer to soil and municipal solid waste compost in a ratio of parts by weight of 4: (1-2). By applying a certain amount of rare earth cerium, the activities of protective enzymes of the lawn plants can be obviously improved, and the defence capability of the protective enzyme system can be enhanced, thus improving the drought resistance of the lawn plants. Efficient and low-cost operation modes in large-area municipal lawn planting are expected to be achieved through the application of the technology.

The invention discloses an effective part extract of valeriana amurensis P.Smirn. and a quality control method and medical use thereof. The content of total lignanoid substances in the extract accounts for over 50 percent of the total weight of the extract, wherein (+) pinoresinol-4,4'-O-beta-D-diglucopyranoside and (+) pinoresinol-8-O-beta-D-glucopyranoside are the main active components in the effective part extract, and the sum of the contents of the two active components accounts for more than 13 percent of the total weight of the extract. The invention also discloses a preparation method and the quality control method of the effective part extract. The effective part extract contains one effective part group of total lignanoid components and can effectively treat central nervous system degenerative diseases including senile dementia, tristimania and the like. The process for preparing the effective part extracts of the valeriana amurensis P.Smirn. for resisting the senile dementia can adapt to industrial production, and provides a new method for treating the central nervous system degenerative diseases by using the valeriana amurensis P.Smirn..

The invention discloses an ultraviolet-visible illumination fruit and vegetable fresh-keeping device and application of the ultraviolet-visible illumination fruit. The ultraviolet-visible illumination fruit and vegetable fresh-keeping device comprises an illumination control system and an illumination internal environment, wherein the illumination control system is arranged on a control platform outside a box body, the illumination control system comprises a control keyboard, a high-precision dimming measuring and control instrument, an illuminance measuring and control instrument, a relative humidity measuring and control instrument and a temperature measuring and control instrument; and the illumination internal environment comprises an ultraviolet light source, an LED (Light Emitting Diode)(red, yellow, blue and green) and other monochromatic sources, and a matched sensor. According to the ultraviolet-visible illumination fruit and vegetable fresh-keeping device, by using an ultraviolet sterilization function and a specific visible light physiological accommodation function, rotting and ageing processes of fruits and vegetables are controlled from two aspects including pathology and physiology, microorganisms carried in the surfaces of the fruits and the vegetables are killed, and the quality and functional ingredients of the fruits and the vegetables are kept, and thus an ideal refreshing effect is achieved.

The invention discloses a composition containing bifidobacterium and application thereof. The composition is characterized by being prepared by mixing the bifidobacterium subjected to high-density culture and dual-layer embedding, oligosaccharide, vitamins, metal elements and auxiliary materials, wherein the composition containing the bifidobacterium comprises the components of 0.01-10 percent of bifidobacterium, 10-50 percent of oligosaccharide, 0.01-10 percent of vitamins, 0.01-10 percent of metal elements and the balance of auxiliary materials. The composition containing bifidobacterium has the functions of assisting in reducing blood fat and resisting oxidation, can be prepared into capsules, electuary, granules, tablets and the other forms and can be applied to the fields of foods, health-care products and medicines.

The invention relates to a method for preserving spinach and belongs to the technical field of vegetable preservation. The method comprises the following steps of: (1), harvesting the spinach which meets a harvesting specification in time; (2), quickly precooling the spinach to be between 4 and 7 DEG C; (3), subpackaging the spinach into a special spinach freshness protection package, fastening a pocket opening, and placing the package into a refrigeration storage with the temperature of between -1 and 2 DEG C and a humidity of 90 to 98 percent; then unfastening the pocket opening after 2 to 3 hours, inserting a blank pipe with an inner diameter of 30mm, then fastening the pocket opening for keeping venting quality of the pocket opening, and automatically adjusting a gaseous environment in the package, wherein the content of CO2 is 2.5 to 8 percent and the content of O2 is 8 to 12 percent; and (4) unfastening the pocket opening every 5 to 7 days serving as a period, ventilating for 2 to 3 hours, and then inserting the blank pipe again for the management of fastening the opening until preservation is completed. The method has low cost, simple and easy operation, good preservation effect, and the spinach keeps excellent commercial quality after 25 days; and the method can be applied to broad vegetable growers, major dealers or processing enterprises.

The invention relates to a rhizoma alismatis lipid-reducing concentrated pill. The pill is mainly prepared from the following raw medicinal materials in parts by weight: 20-30 parts of rhizoma alismatis, 6-12 parts of emblic leafflower fruit, 6-12 parts of fried rhizoma atractylodis macrocephalae, 6-12 parts of red yeast rice, 3-9 parts of lotus leaf and 3-9 parts of hawthorn. The invention aims to provide the rhizoma alismatis lipid-reducing concentrated pill for treating hyperlipidaemia, which is efficient, safe, low in price and convenient to take. The rhizoma alismatis lipid-reducing concentrated pill has the advantages that 1, the rhizoma alismatis lipid-reducing concentrated pill has the effects of promoting diuresis and strengthening spleen, activating blood circulation and removing food retention, and can be used for treating patients with syndromes of phlegm and stasis stagnation and dysfunction of spleen in transport caused by hyperlipidaemia; 2, the rhizoma alismatis lipid-reducing concentrated pill has the effects of improving dyslipidemia, cleaning free radicals in bodies, reinforcing body oxidation resistance and reducing liver fatty degeneration, and can be used for preventing and treating hyperlipidaemia, atherosclerosis, fatty liver and hyperlipidaemia related diseases.

The invention provides application of guluronic acid oligomers in preparing medicines, health products or dietary supplements for preventing and treating alcoholic liver injury. Guluronic acid oligomers with different molecular weights, which are obtained by degrading polyguluronic acid graded and purified from algin, can lower the hepatosomatic index increase caused by alcoholic injury, resist triglyceride and cholesterol increase caused by alcohol, effectively lower glutamic-pyruvic transaminase and glutamic oxaloacetic transaminase increase in serum caused by alcohol, enhance the total oxidation resistance, glutathione level and superoxide dismutase activity in the serum, inhibit the generation of malonaldehyde in the liver caused by alcohol, lower the mouse drunkenness rate, prolong the mouse drunkenness time, and shorten the sober-up time, thereby implementing the protective action and hangover alleviating function on alcoholic hepatic injury. The products provided by the invention are derived from marine natural products, have the advantages of abundant resources, high industrialization tendency, high safety, high effectiveness and the like, and have wide development and application prospects in the aspects of prevention and treatment of alcoholic hepatic injury, hangover alleviation and liver protection.

The invention discloses lucid ganoderma-corydalis saxicola compound chewable tablets. The formula of the lucid ganoderma-corydalis saxicola compound chewable tablets comprises 10-25 parts of lucid ganoderma, 10-15 parts of fructus lycii, 2-6 parts of corydalis saxicola, 5-10 parts of fructus momordicae, 20-30 parts of hydroxypropyl methyl cellulose, 5-15 parts of maltodextrin and 1-3 parts of lubricant. A production method of the lucid ganoderma-corydalis saxicola compound chewable tablets comprises the following steps: grinding the raw materials separately and sieving with a 500-mesh sieve to obtain ultrafine powder; combining according to the formula and stirring uniformly; granulating with alcohol by a wet process; drying at 60-80 DEG C until the water content reaches 4-6% by weight; cooling to room temperature, and sieving with a 60-mesh sieve; adding the lubricant and tabletting to obtain the lucid ganoderma-corydalis saxicola compound chewable tablets. The chewable tablets disclosed by the invention have the healthcare effects of clearing heat and removing toxin, protecting and nourishing liver, adjusting immunity, reducing blood sugar and blood fat, resisting oxidation and aging, replenishing vital essence to improve eyesight, promoting longevity and the like.

The invention belongs to the technical field of pesticides and in particular to relates to a multifunctional medicinal plant seed coating agent and preparation and coating methods thereof. The multifunctional medicinal plant seed coating agent comprises active ingredients, auxiliary agents and water. The multifunctional medicinal plant seed coating agent comprises the following raw materials in percentage by mass: the active ingredients: 6-18% of a bactericide, 5-15% of an insecticide, 3-6% of a balancing fertilizer and 0.03-0.04% of a growth regulator; 11-19% of the auxiliary agents; and the balance of water, totaling 100%. According to the technical scheme, the invention also provides the preparation method of the coating agent. The method is simple and feasible and easy to be industrially popularized.

The invention discloses an anti-radiation traditional Chinese medicine composition which comprises the following components in parts by weight: 160-200 parts of membranous milkvetch root, 110-130 parts of scurfy pea, 80-100 parts of Chinese angelica, 160-200 parts of epimedium, 135-165 parts of madder root, 110-130 parts of glossy privet fruit, 110-130 parts of white paeony root, 110-130 parts ofradix rehmanniae preparata, 80-100 parts of Szechuan lovage rhizome, 215-265 parts of Chinese yam and 35-45 parts of prepared liquoric root. The traditional Chinese medicine composition can increase thymus index and spleen index of a 60Co gamma-radiated mouse and protect immune organs; improve the activity of superoxide dismutase in blood serum and the liver of the 60Co gamma-radiated mouse, reduce the content of malonaldehyde and improve the anti-oxidation capability of an organism; increase peripheral white blood cells of the 60Co gamma-radiated mouse and improve the hematogenesis function;and reduce the micronuclear rate of marrow cells of the 60Co gamma-radiated mouse and inhibit the action of causing chromosomal aberration of 60Co gamma rays. In addition, the traditional Chinese medicine composition is low in toxicity and side effects, and medicinal materials in the formula are low in price and easy to obtain, thereby having great development and application prospects in the aspects of preventing and treating radiation injuries.

The invention discloses a process for extracting sphallerocarpus gracilis polysaccharide through a complex enzyme method. The process comprises the following steps: drying and grinding sphallerocarpus gracilis, adding water, implementing microwave extraction, hydrolyzing through cellulase and hydrolyzing through papain, conducting enzyme deactivation, cooling, centrifuging and reserving a supernatant liquid, extracting through two layers of filter paper, decompressing and concentrating, precipitating through ethanol, and freeze-drying to obtain the sphallerocarpus gracilis polysaccharide, wherein polysaccharide yield is 18.92-21.35%. Through a phenol-sulfuric acid method, polysaccharide content in an extract is detected to be 88.33%+ / -0.93%, and through coomassie brilliant blue, protein content is tested to be 2.17%+ / -1.03%. Experiments show that the sphallerocarpus gracilis polysaccharide has a remarkable inhibiting effect on blood sugar and blood fat of experimental hyperglycemic rats, and is safe and free from side effect. After being ground, the freeze-dried extract is prepared into tablets, hard capsules, granules and the like; and the sphallerocarpus gracilis polysaccharide is a natural healthcare product having a function of regulating blood sugar and blood fat.

The invention relates to a traditional Chinese medicine for reducing the blood fat, and a preparation method thereof. The traditional Chinese medicine comprises: a dry extract paste of extracts obtained through carrying out water decoction extraction or ethanol reflux extraction on mixed Rhizoma Alismatis, haw, Gynostemma pentaphylla, Radix Puerariae and Lycium Chinense; and Panax Notoginseng powder. The preparation method comprises the following steps: taking and mixing the Rhizoma Alismatis, the haw, the Gynostemma pentaphylla, the Radix Puerariae and the Lycium Chinense, extracting through decocting with water or refluxing with ethanol to obtain an extract solution, and concentrating the extract solution to obtain the dry extract paste; selecting Panax Notoginseng, drying, carrying out radiant quantity disinfection, and crushing to 80 mesh fine powder; and adding the water or ethanol extraction dry extract paste and the Panax Notoginseng powder to a mixer together, and mixing to obtain the traditional Chinese medicinal powder for reducing the blood fat of the invention. The obtained medicinal powder can be filled to capsules or added with other routine medicinal carriers or excipients to prepare usual oral medicinal forms. Hyperlipidemia belongs to the 'turbid phlegm' category of the traditional Chinese medicine, so the traditional Chinese medicine screens the composition according to dampness removing, turbidity transforming, stasis scattering and vein relaxing principles, can nourish blood in the liver and the kidney, and also can prevent extremeness disadvantages of the haw and the Gynostemma pentaphylla, thereby the new traditional Chinese medicine with a substantial curative effect is provided for treating the hyperlipidemia.

The invention relates to a Chinese herbal medicinal compound preparation used for treating macular degeneration of old people and a preparation method thereof. The Chinese herbal medicinal compound preparation disclosed by the invention is in various medically acceptable dosage forms such as oral liquid and solid preparations comprising tablets, granules and capsules which are composed of extracts of ten traditional Chinese medicinal materials, namely rehmannia glutinosa, root bark of tree peony, poria, Chinese yam, rhizoma alismatis, cornus, cinnamon, monkshood, bat dung and rhizoma atractylodis, and pharmaceutic adjuvants. The invention also discloses a preparation method of the Chinese herbal medicinal compound preparation. The Chinese herbal medicinal compound preparation disclosed by the invention has the effects of tonifying kidnety and warming yang, replenishing qi to invigorate the spleen, invigorating blood circulation to promote the secretion of saliva or body fluid and dredging collaterals to improve eyesight and has obvious therapeutical effect on the macular degeneration of the old people.

The invention discloses a bio-composite anti-mold preservative for citrus. The bio-composite anti-mold preservative for the citrus disclosed by the invention consists of the following raw materials in part by weight and volume: 1 part of glacial acetic acid, 2 to 5 parts of chitosan, 1.5 to 2 parts of bacillus amyloliquefaciens HRH.317 fermentation liquid, 1.5 to 2 parts of Tween-80, 0.3 to 0.5 part of NaCl, and 93 parts of distilled water. The preparation method of the preservative comprises the following steps: firstly, adding glacial acetic acid into the distilled water to prepare acetic acid solution; then, respectively adding the chitosan, the bacillus amyloliquefaciens HRH.317 fermentation liquid, the Tween-80 and the NaCl into the acetic acid solution; and finally, stirring the mixture uniformly. The using method of the preservative comprises the following steps: washing the citrus without damage or disease with tap water, and immersing the citrus into 0.1 percent sodium hypochlorite solution and sterilizing for 1 to 2 minutes, taking the citrus out, cleaning the sodium hypochlorite adhered to the citrus by using sterile water, drying the citrus, completely immersing the citrus into the preservative for 30 to 40 minutes, fishing out the citrus, and packaging the citrus by using PE (polyethylene) freshness protection bags; packaging 25 to 40 citruses in each bag; arranging the packaged citruses in plastic baskets for storage under the conditions that the room temperature is 18 to 23 DEG C and the indoor relative humidity is 70 to 80 percent, or under the conditions that the storage temperature is 4 to 6 DEG C and the relative humidity is 70 to 80 percent.

A regulator for growing se-enriched tea leaves is prepared through fermenting pig dung and silkworm excrement, filtering, adding sodium selenate (or selenite) and dissolving. Its advantage is high Se content in tea leaf. It is applied through diluting and spraying it on buds of tea tree.