16results about How to "Reduce MDA content" patented technology

This invention relates to the field of food preservation technology and discloses a liquid preservative for preserving fresh walnut kernels. The preservative comprises a natural preservative, a solubilizing and stabilizing agent, and a solvent. This liquid preservative uses natural phloretin as the preservative component, combined with Tween 80 as a solubilizing and stabilizing agent and ethanol as a solvent. This effectively solves the problems of poor water and oil solubility and inadequate stability of phloretin, allowing it to be stably dispersed in the preservative system and fully exert its antioxidant and antimicrobial effects. Furthermore, the preparation method is simple and easy to implement, and the production process is safe and controllable. It is convenient to use; the preservation of fresh walnut kernels can be achieved through dilution and soaking. The diluted solution can be reused, reducing usage costs. Simultaneously, it can inhibit bacterial growth during the storage of fresh walnut kernels, delay oxidative deterioration, maintain their good appearance and quality, extend shelf life, and meet the market demand for high-quality fresh walnut kernels.

This invention belongs to the field of biotechnology, specifically relating to the cassava transcription factor MeWRKY30 and its applications. This invention cloned the transcription factor gene of MeWRKY30 from cassava. Overexpression of this gene in cassava can improve the resistance of transgenic cassava plants to low-temperature and drought stress. The effects of qRT-PCR on cassava... MeWRKY30 Gene expression was significantly upregulated after drought and treatment with the plant hormone abscisic acid (ABA). After recovery from low temperature treatment, the malondialdehyde content of the overexpressing cassava lines decreased, wilting was reduced, root growth inhibition was lessened, and the number of cortical cells in the root tip meristem was significantly increased.

The application discloses a salt and alkali tolerant bacterial strain and application thereof. The salt and alkali tolerant bacterial strain is selected from any one or more of the following: Pseudoclavibacter sp. with a preservation number of CGMCC No. 32646, Providencia sp. with a preservation number of CGMCC No. 32647, and Planococcus sp. with a preservation number of CGMCC No. 32648, which are all preserved in the China General Microbiological Culture Collection Center (CGMCC) on November 15, 2024, and the preservation address is No. 1, Xibanchenli, Chaoyang District, Beijing, China. All of them have the abilities of nitrogen fixation and indole acetic acid production; can significantly promote the growth of plants in salt and alkali, improve the content of chlorophyll and proline in rice, improve the antioxidant enzyme activity, and reduce the content of malondialdehyde; in addition, the Providencia sp. has the ability of nitrogen fixation, the Pseudoclavibacter sp. has the ability of iron production, and the Planococcus sp. has the abilities of iron production and phosphorus dissolution.

The invention relates to the field of food processing, in particular to a preparation method of whole-plant fried ginseng and application of the whole-plant fried ginseng in preparation of drugs or food for resisting oxidation and relieving fatigue. The method comprises the following steps: fresh ginseng is taken and cleaned, rhizome, main roots and fibrous roots are separated, the main roots are cut into slices or segments, the rhizome, the slice-shaped main roots or the segment-shaped main roots and the fibrous roots are respectively placed in oil to be fried, and when the rhizome, the slice-shaped main roots or the segment-shaped main roots and the fibrous roots are golden yellow, the rhizome, the main roots and the fibrous roots are fished out, drained off oil and cooled. The antioxidant activity of the fried ginseng slices and segments is improved. Compared with fresh ginseng, the deep-fried ginseng slices and segments can significantly prolong exhaustive swimming time of load-bearing mice, significantly improve liver / muscle glycogen, lactic dehydrogenase content and superoxide dismutase activity, reduce lactic acid, urea nitrogen and malondialdehyde content, and have a significant anti-fatigue effect. According to the method, dryness of fresh ginseng can be removed, the eating fragrance can be improved, the fried ginseng product is instant and convenient to eat, and the fried ginseng product is matched with barbecue kebabs and stewed together with livestock and poultry meat, so that the fried ginseng product is delicious and health-care.

This invention belongs to the field of traditional Chinese medicine preparations, and relates to the preparation method and application of a compound preparation of Yao Yu effervescent tablets with anti-rheumatoid arthritis effect. The compound preparation comprises the following traditional Chinese medicine components: 4 parts by weight of Dioscorea cirrhosa, 3 parts by weight of Tinleaf Vine, 2 parts by weight of Camphor Tree, 2 parts by weight of Rhus chinensis, 3 parts by weight of Rhododendron molle, 1 part by weight of Ephedra sinica, 2 parts by weight of Semi-Acer palmatum, 2 parts by weight of Gynostemma pentaphyllum, and 1 part by weight of Cinnamomum cassia. The preparation steps include: placing Dioscorea cirrhosa, Tinleaf Vine, Camphor Tree, Rhus chinensis, Rhododendron molle, Gynostemma pentaphyllum, Semi-Acer palmatum, Gynostemma pentaphyllum, and Cinnamomum cassia in 6000 parts by weight of 70% ethanol. Extract for 2 hours, repeat three times, filter, and combine the filtrates; concentrate, freeze-dry, pulverize, and pass through an 80-mesh sieve for later use; mix 27.5% freeze-dried powder with 26.45% citric acid, 34.11% sodium bicarbonate, 5.61% PEG6000, and 6.33% lactose evenly, pass through an 80-mesh sieve, and prepare a soft mass using anhydrous ethanol as a wetting agent; pass the soft mass through a 20-mesh sieve and dry at 37℃ for 30 minutes to obtain compound preparation granules; granulate through a 20-mesh sieve for granulation, add lubricant PEG6000 and mix evenly; compress into tablets to obtain compound preparation effervescent tablets. This invention, through pharmacological experiments, confirms that the Yaoyu effervescent tablet compound preparation has a good anti-rheumatoid arthritis effect: it can reduce local inflammatory mediators and serum IL-1β, IL-6, TNF-α, and PEG2 levels, downregulate MDA and increase SOD to improve oxidative stress, inhibit the expression of MMP3, COX-2, and P38 proteins in the synovial membrane, and reduce pathological tissue damage. The medium and high dose groups showed the best effect, and it has a good therapeutic and protective effect on the ankle joint of AA rats, with good clinical application value.

This invention belongs to the field of genetic engineering technology, specifically involving TaSCAMP17 The application of its recombinant overexpression vector in improving plant salt tolerance, the TaSCAMP17 The nucleotide sequence is shown in SEQ ID NO.1, and the amino acid sequence is shown in SEQ ID NO.2. This invention will... TaSCAMP17 Functional validation was performed in Arabidopsis thaliana after overexpression, and the results showed that overexpression... TaSCAMP17 The salt tolerance of wild-type Arabidopsis thaliana is significantly improved, therefore TaSCAMP17 It plays a positive regulatory role in wheat salt tolerance, providing a theoretical basis and gene resources for wheat molecular breeding, and is of great significance for obtaining salt-tolerant and high-yielding lines and improving wheat salt tolerance in the future.

The present application relates to a kind of soybean polypeptide preparation method with antioxidant and antagonistic lead damage effect.The soybean polypeptide prepared in the present application has good DPPH free radical clearance rate, ABTS free radical clearance rate and Fe3+ reduction capacity in vitro;It can also alleviate the case that the body weight of mouse is slowly increased due to lead exposure, improve the learning and memory ability of lead exposure mouse, alleviate the organ damage of mouse caused by lead exposure, enhance the level of reduced glutathione, superoxide dismutase and catalase of organism, and alleviate the abnormal increase of malondialdehyde caused by lead exposure.The soybean polypeptide prepared in the present application has good in vitro antioxidant capacity, and has antagonistic effect on oxidative stress caused by lead exposure mouse, and can be used as non-toxic and harmless natural antioxidant.For the development of natural antioxidant food, it has far-reaching application prospect, and also provides a new feasible idea for the combined application of bioinformatics technology and traditional enzymatic technology.

The invention provides Pseudomonas kirscheri WF3, a microbial fertilizer for saline-alkali soil and a preparation method and application of the microbial fertilizer, and belongs to the technical field of biological composting. The invention provides a preparation method of a microbial fertilizer for saline-alkali soil. The preparation method comprises the following steps: (1) preparing a fertilizer carrier comprising a decomposed organic fertilizer, humic acid, attapulgite powder and polyglutamic acid; and (2) inoculating pseudomonas kirschner WF3 into the fertilizer carrier, and carrying out sealed fermentation to obtain the microbial fertilizer for saline-alkali soil. Pseudomonas kirschner WF3 which has exopolysaccharide and organic acid production capacity and can survive in a strong saline-alkaline environment is adopted for fermentation of a fertilizer carrier, the prepared microbial organic fertilizer can effectively improve the content of organic carbon, total nitrogen and water-stable large aggregates (larger than 2.5 mm) in soil, increase the yield of corn, reduce the pH and total salt content of the soil, and improve the yield of corn. The improvement effect on the saline-alkali soil is obvious.

This invention discloses a novel use of Artemisia annua seeds, a medicinal and edible compound composition for relieving hangovers and protecting the liver, chewable tablets, and their preparation process. This invention is the first to discover that the mucilage in Artemisia annua seeds slows down ethanol diffusion through a dual mechanism of physical adsorption and three-dimensional network blockage, thus slowing down the rate of ethanol absorption in the digestive tract, reducing the peak blood plasma concentration, and delaying or prolonging the time of intoxication. Therefore, it can be used in the preparation of health products / functional foods that protect the stomach, prevent intoxication, and delay alcohol absorption. Based on this discovery, this invention provides a medicinal and edible compound composition for relieving hangovers and protecting the liver, composed of 10-15 parts by weight of Pueraria lobata, 10-15 parts by weight of Hovenia dulcis, 8-10 parts by weight of Lilium brownii, 4-8 parts by weight of Poria cocos, 4-8 parts by weight of Dioscorea opposita, and 4-8 parts by weight of Artemisia annua seeds. Furthermore, to balance rapid effectiveness and compliance, it is formulated as a chewable tablet. This chewable tablet can significantly prolong the time of intoxication and shorten the sobering-up time, and is significantly superior to the group lacking Artemisia annua seeds in terms of antioxidant indicators (SOD, GSH, MDA) and hangover relief effect.

The application discloses a kind of biological active peptide capable of improving sea cucumber summer passing capacity and its preparation method and application.The biological active peptide is obtained by extracting, enzyme hydrolysis, separation and purification using tuna meat as raw material, and the amino acid sequence of the active peptide is MADFFTRL.Test shows that the biological active peptide can improve the survival rate of sea cucumber under high temperature stress, enhance the activities of SOD, CAT and other antioxidant enzymes, reduce the content of MDA, reduce the oxidative damage caused by high temperature, thereby improving the heat resistance and safe summer passing capacity of sea cucumber.The biological active peptide can be used for preparing sea cucumber feed additive or functional feed, and has good application prospect.

The invention discloses preparation of an immobilized material based on phosphate solubilizing bacteria and application of the immobilized material in soil, and belongs to the technical field of soil remediation microbial agents. The material takes bacillus megatherium and lignin as core functional components, and takes a mixed system of sodium alginate and sodium carboxymethyl cellulose as a carrier. The immobilized material is high in mechanical strength, good in stability and excellent in mass transfer efficiency, meanwhile, insoluble phosphorus is activated through metabolites secreted by bacillus megatherium, and the content of available phosphorus in soil is increased. The material can also improve the soil structure, improve the cation exchange capacity and the organic matter content, enhance the activities of peroxidase and superoxide dismutase of the rice, reduce the content of malonaldehyde, relieve oxidative damage caused by Cd stress, reduce Cd accumulation of the rice from the source and guarantee normal growth of the rice.

The application discloses a preparation method of whole wheat flour with controlled lipase and lipoxygenase activity, and relates to the technical field of whole wheat flour processing and storage. The method comprises the following steps: adopting DBD plasma to treat the bran of wheat grains, and then directly grinding the wheat grains to obtain the whole wheat flour. The method controls the lipase and lipoxygenase in the bran of the wheat grains, thereby stabilizing the whole wheat flour, prolonging the storage period of the whole wheat flour, and improving the nutritional quality and processing quality of the whole wheat flour. The DBD plasma treatment is performed at a power of 300-500 W for 60-180 s.

This invention relates to a salt tolerance-related protein, LcCIPK25, and its encoding gene in hybrid tulip tree (Liriodendron tulipifera), and its applications, belonging to the field of plant genetic engineering technology. The amino acid sequence of this protein is shown in SEQ ID NO.2, and the nucleotide sequence of the gene encoding this protein is shown in SEQ ID NO.1. This invention also provides a recombinant expression vector containing the aforementioned nucleic acid molecule, a recombinant host cell, and its application in improving plant salt tolerance. Overexpression of the aforementioned nucleic acid molecule in hybrid tulip tree can maintain CAT activity, alleviate leaf yellowing and oxidative damage under high salt stress, significantly reduce MDA content, and effectively enhance the plant's salt tolerance.

The application discloses a planting method for relieving drought of wheat based on combined application of SiQDs seed dressing and halophilic bacillus, and belongs to the technical field of agricultural planting. The application discloses a planting method for relieving drought of wheat based on combined application of SiQDs seed dressing and halophilic bacillus, which comprises the following steps: using silicon quantum dots (SiQDs) for seed dressing treatment of wheat seeds, then planting the seed-dressed wheat seeds in soil, and applying halophilic bacillus agent during the growth of the wheat. The combined application of SiQDs and halophilic bacillus GS127 can better promote the root development of wheat under drought stress, increase the content of proline and soluble sugar in the wheat, reduce the content of malondialdehyde, enhance the drought resistance of the wheat, effectively promote the growth of the wheat, and improve the yield and water use efficiency, and therefore has excellent application potential in the production in agricultural wheat drought areas.

This invention discloses the application of the rice low-temperature tolerance-related bZIP transcription factor OsbZIP65 in cultivating stress-tolerant plants, belonging to the field of bio-agricultural technology. This invention relates to a newly identified bZIP transcription factor in rice. OsbZIP65 Low-temperature resistance. Transgenic experiments have shown that... OsbZIP65 Overexpression lines significantly improved the low-temperature tolerance of rice, while knockout lines showed increased sensitivity to low temperatures. These studies indicate that... OsbZIP65 It plays a crucial role in regulating low-temperature tolerance in rice. This invention reveals through functional analysis... OsbZIP65 The potential of gene application in improving rice's low-temperature tolerance provides technical support for genes and their applications, aiming to provide new theoretical basis for breeding rice varieties with strong stress resistance and wide adaptability, and to contribute to sustainable agricultural development and global food security.