133 results about "Functional validation" patented technology

The invention discloses cytosine deaminase as well as a related biological material and application thereof, and belongs to the technical field of protein. The technical problem to be solved by the invention is to excavate natural cytidine deaminase without sequence preference and improve the base editing efficiency and range. The invention provides a 15th-143rd site protein of which the amino acid sequence is SEQ ID NO.2. The invention also provides a preparation method of the protein. The invention also provides application of the protein and related biological materials thereof in single base editing. According to the application, metagenome big data is utilized, comprehensive and systematic information mining analysis is carried out on novel cytidine deaminase, and a protein L85 which is highly similar to a known DddA protein structure but low in sequence similarity is selected from the novel cytidine deaminase for functional verification. The protein L85 has high catalytic activity as cytidine deaminase, is not limited by target sequence preference, can be widely applied to gene editing in vitro and in cells / tissues, and has good application potential and development value.

The invention discloses application of a corn ZmTST1 gene in improvement of plant yield and stress resistance. The nucleotide sequence of the corn ZmTST1 gene is as shown in SEQ ID NO. 1; the stress resistance is tolerance to salt stress and drought stress. Through sequence analysis and functional verification, after the ZmTST1 gene is over-expressed, the growth of plants can be promoted, the yield of the plants can be increased, meanwhile, the drought tolerance and the salt tolerance of ZmTST1 expressed plants are obviously higher than those of wild plants, and it is proved that the ZmTST1 gene has a remarkable effect in the aspects of high yield and stress resistance. By identifying the ZmTST1 gene, plants with high stress resistance can be screened, the cultivation period of the plants can be shortened, and a new method and thought are provided for plant breeding in the future.

The invention belongs to the technical field of plant genetic engineering, and discloses application of an OsSRO1c gene in controlling high-temperature resistance of rice. The OsSRO1c gene capable of improving the drought tolerance of rice is obtained through separation, cloning and functional verification, the nucleotide sequence of the OsSRO1c gene is as shown in SEQ ID NO.1, and the sequence of protein coded by the gene is as shown in SEQ ID NO.2. The gene OsSRO1c for controlling rice high-temperature response is cloned, CRISPR mutant phenotype identification is carried out on candidate genes, high-temperature stress phenotype identification in a seedling stage and an adult-plant stage shows that when a gene segment is deleted, the high-temperature resistance of rice is reduced, and when the expression quantity of the gene is increased, the high-temperature resistance of rice is improved, and the function and the application way of the gene are proved.

The invention belongs to the technical field of biology, and particularly relates to casein phosphopeptides for promoting absorption of calcium, iron and zinc minerals and improving bone health. Casein phosphopeptides containing high-activity peptide fragments are obtained through an optimization process, six mineral elements of calcium, magnesium, copper, iron, zinc and manganese serve as research objects, firstly, mineral utilization of CPP is evaluated through a mineral deficiency rat model, the content of Ca, Mg, Cu, Fe, Zn and Mn in thighbones is increased by 7.12%, 3.56%, 20.76%, 15.84%, 9.94% and 67.12% respectively when the CPP is eaten by 7mg / kg.d; secondly, screening a peptide fragment with calcium absorption promoting activity from the CPP by utilizing a molecular docking technology, and performing functional verification; the invention aims to reveal the action effect of CPP in mineral absorption and utilization from cell and animal levels, and provides a theoretical basis for functional development of CPP.

The invention discloses an EsSTB1 gene capable of increasing the number of branches of a plant, reducing the plant height and delaying flowering. The EsSTB1 gene has the functions of increasing the number of branches, reducing the plant height and delaying flowering, and a scientific basis and a new material are provided for cooperative regulation and control of tillering formation and reproductive growth of siberian wildrye; besides, the functional verification method of the EsSTB1 gene provided by the invention performs functional verification by applying transgenic arabidopsis thaliana starting from an internal regulation mechanism of tillering regulation of siberian wildrye, has obvious research depth and breadth and technical effects, and can obtain a plant strain with an intentional phenotype. Through verification, the siberian wildrye EsSTB1 gene disclosed by the invention can be used for remarkably increasing the number of branches of a plant, reducing the plant height and delaying flowering. The method is suitable for popularization and application in the biotechnology field.

The invention discloses a heptapeptide with a muscle building function as well as preparation and application thereof, and belongs to the technical field of biological medicines. The amino acid sequence of the heptapeptide SR7 is Ser-Ser-Gly-Lys-Phe-Leu-Arg, and the amino acid sequence of the heptapeptide SR7 is as shown in the specification. The heptapeptide can be obtained through artificial synthesis or directional enzymolysis of wheat protein. Functional verification shows that the heptapeptide SR7 and the wheat peptide obtained through directional enzymolysis have the muscle building function which is mainly reflected in enhancing the exercise ability, including increasing the total exercise distance, the moving speed and the activeness frequency; and increase muscle strength and muscle mass. And the food-borne bioactive peptide is high in biological safety. Therefore, the compound can be applied to preparation of medicines for improving sarcopenia or health-care foods for increasing muscle. The invention provides a novel active substance with a muscle building function, and the novel active substance has a good market prospect and application potential.

The invention relates to the technical field of medicines, and particularly discloses a screening target of an antibacterial drug, application and a screening method, and the screening method comprises the following steps: testing the interaction between a candidate drug and the target; functional verification of the target protein; testing the antibacterial activity of the candidate drugs; and carrying out an in-vitro antibacterial curve test, evaluating the sterilization or antibacterial effect of the candidate drug on different bacteria, and comparing the change of bacterial gene expression before and after the treatment of the candidate drug through omics analysis (transcriptome and proteome analysis). A new direction is provided for research and development of antibacterial drugs by discovering a new antibacterial target, such as Spx and SodA, of the ISP I except for acting on bacterial ribosomes, a new drug without cross resistance with traditional antibiotics can be developed according to the drug design of the new target, the antibacterial effect is improved, the new antibacterial target of the ISP I in the colimycin and the action mechanism of the new antibacterial target are disclosed, and the application of the ISP I in the colimycin in preparation of the antibacterial drugs is promoted. And a new thought and a new method are provided for research and development of antibacterial drugs.

The invention belongs to the technical field of biology, and particularly relates to casein source heptapeptide for promoting calcium absorption and utilization and application of the casein source heptapeptide. The CPP containing the high-activity peptide fragment is obtained through an optimization process, and the influence of the CPP on mineral absorption and conversion is evaluated by utilizing a mineral deficiency rat model; then, heptapeptide with calcium absorption promoting activity is screened out from the CPP in combination with peptiomics and molecular docking technologies, functional verification is carried out, it is found that when the VAPFPEV is 2 mu M, the Ca < 2 + > transport amount is remarkably increased by 25.25%, meanwhile, zebra fish experiments show that intervention of the VAPFPEV effectively reverses reduction of an accumulated optical density value caused by dexamethasone, and compared with a module making set, the VAPFPEV has the advantages that the VAPFPEV has the advantages that the VAPFPEV has the advantages that the VAPFPEV has the advantages that the VAPFPEV has the advantages that the VAPFPEV has the The skull accumulative optical density value of a VAPFPEV (1-20 [mu] M) processing group is obviously increased. It is shown that VAPFPEV can effectively promote Ca < 2 + > transfer and absorption, improve the osteoporosis symptom of zebra fish and increase deposition of calcium in bones.

The invention relates to the technical field of microbiology, in particular to a biological reagent research and development method based on microbial functional gene screening. The method comprises the following steps: acquiring a microorganism sample, and performing genome sequencing extraction treatment and gene transcription analysis to generate transcriptome data of the microorganism sample; obtaining a biological reagent research and development target function, and performing target function gene screening treatment and gene targeted knockout treatment to generate a target function gene targeted knockout experimental group; carrying out gene cloning on the target functional gene targeted knockout experimental group, transferring into a preset host strain, carrying out function expression evaluation analysis to obtain a target functional expression level corresponding to each target functional targeted knockout gene, and carrying out excellent function screening and verification to generate a biological reagent excellent function verification gene, and the method is applied to a research and development process corresponding to a biological reagent so as to generate a target functional biological reagent product. The functional screening efficiency and the specificity of biological reagent products can be improved.

The invention belongs to the technical field of gene engineering, and particularly relates to application of a wheat gene to regulation and control of plant drought tolerance. The wheat gene provided by the invention is a TaANK-TPR44 gene, and the nucleotide sequence of the TaANK-TPR44 gene is as shown in SEQ ID NO. 1. In order to develop the specific functional characteristics of ankyrin genes from different plant sources, the TaANK-TPR44 gene is over-expressed and transformed into arabidopsis thaliana for functional verification, and the result shows that the drought tolerance of the arabidopsis thaliana with the over-expressed TaANK-TPR44 gene is remarkably improved compared with that of wild arabidopsis thaliana, and the drought tolerance of the arabidopsis thaliana with the over-expressed TaANK-TPR44 gene is remarkably improved compared with that of wild arabidopsis thaliana. Therefore, the TaANK-TPR44 gene plays a positive regulation role in the wheat drought tolerance process, a theoretical basis and gene resources are provided for wheat molecular breeding, and the TaANK-TPR44 gene has very important significance for obtaining drought-tolerant high-yield strains and improving the wheat drought tolerance in the future.

The invention relates to the technical field of biological function prediction, in particular to a biological reagent function prediction method and system. The method comprises the following steps: carrying out biomolecule information acquisition on a biological reagent public database to obtain a biological reagent biomolecule information set; performing biological characteristic analysis on the biological reagent biomolecule information set to obtain biological reagent protein sequence comparison characteristics, biological reagent molecular structure domain characteristics and biological reagent specific molecular function domain characteristics; constructing a biological reagent function prediction network framework and performing function prediction model training construction to generate a biological reagent function prediction model; obtaining molecular characteristics of the biological reagent to be predicted and performing target function prediction to obtain a target biological reagent function prediction result; and performing function verification optimization according to the target biological reagent function prediction result to generate a target biological reagent function optimization result. According to the invention, effective pre-judgment and screening of biological reagent functions can be realized.

The present application provides an isolation, cloning, functional verification of a restorer gene OsRf19 for cytoplasmic male sterility in rice and its use in improving rice. The present application specifically provides a nucleotide sequence of the gene OsRf19 and an encoded amino acid sequence thereof, an expression vector comprising the nucleotide sequence of the gene OsRf19, a method of creating a restorer line of rice using the gene OsRf19, and a method of restoring the fertility of a cytoplasmic male sterility line of rice and producing rice hybrid seeds, and related uses.

The application provides a rice heart-white gene WCG1 and application thereof, and particularly relates to positioning, cloning, function verification and application of a WCG1 gene for controlling a rice kernel heart-white phenotype. A C / A natural variation exists at a ninth exon 986 site of the WCG1 gene, the variation site makes the rice kernel present a heart-white phenotype, that is, a kernel middle heart-white and edge transparent phenotype, and the phenotype is an excellent phenotype of "sake brewing good suitable rice" specially selected in sake brewing.

The application discloses cytosine deaminase and related biological materials and applications thereof, and belongs to the technical field of proteins. The technical problem to be solved by the application is to mine natural sequence-unpreferred cytosine deaminase, and to improve base editing efficiency and range. The application provides a protein with amino acid sequence of SEQ ID NO. 2 at positions 15-143. The application also provides application of the above protein and related biological materials thereof in single base editing. The application uses macro-genome big data, performs comprehensive and systematic bioinformatics mining analysis on novel cytosine deaminase, and selects a protein L85 highly similar to known DddA protein structure but with low sequence similarity for function verification. The protein L85 has high catalytic activity as cytosine deaminase, and has no target sequence preference restriction, and can be widely applied to in-vitro and in-cell / tissue gene editing, and has good application potential and development value.

The invention discloses a Chinese cabbage clubroot-resistant gene BrRLP31 and application of the Chinese cabbage clubroot-resistant gene BrRLP31. The nucleotide sequence of the Chinese cabbage clubroot-resistant gene BrRLP31 is as shown in SEQ ID No. 1. Functional verification results show that after the expression of the endogenous BrRLP31 of the Chinese cabbage is inhibited by utilizing a VIGS technology, the resistance of the plant to clubroot is obviously reduced, and the disease index and the root swelling severity of the plant are obviously higher than those of a control material. On the other hand, the transgenic flowering cabbage plant over-expressed by the BrRLP31 is constructed by an agrobacterium-mediated method, and disease resistance identification proves that the heterologous over-expression of the BrRLP31 obviously improves the resistance of the plant to plasmodiophora brassicae. The results show that the BrRLP31 gene plays a key positive regulation role in the clubroot resistance process of the Chinese cabbage, the disease resistance of cruciferae crops and other horticultural plants is improved by utilizing the gene and the homologous sequence thereof, and the gene and the homologous sequence thereof have important application value.

The invention discloses a non-specific peroxygenase mutant and application of the non-specific peroxygenase mutant. According to the method, Collariella virescens UPO of which the protein structure is analyzed is rationally designed by utilizing a multiple computer-aided strategy, and through functional verification, a UPO mutant library which has rich enantioselectivity to various substrates and is improved in activity is successfully obtained, so that a solid foundation is laid for researching and developing a green and efficient chiral compound production method. Compared with the prior art, the non-specific peroxygenase mutant provided by the invention shows high catalytic activity in selective oxidation of carbon-hydrogen bonds of various substrates, the selectivity is remarkably improved or overturned, the obtained reaction product is high in optical purity, the reaction efficiency is improved, the separation process is simplified, the cost is reduced, and the non-specific peroxygenase mutant is suitable for industrial production. Good industrial application prospects are realized.

The application discloses a kind of BRCA1 / 2 The application discloses a function verification method of genetic unknown meaning variation. By introducing only one target variation in each experiment, combined with optimized gRNA and template design, the method ensures that the target variation has high initial abundance in the edited cell population, thereby significantly reducing the background noise of second-generation sequencing detection and phenotype analysis, improving the sensitivity and reliability of variation function signal detection. The method provides a more flexible, accurate and easy-to-operate function verification scheme for BRCA1 / 2 The method provides a more flexible, accurate and easy-to-operate function verification scheme for interpretation of unknown meaning variation, and is expected to solve the bottleneck of difficulty in obtaining and interpreting function data of unknown meaning variation.

The invention belongs to the technical field of gene engineering, and particularly relates to application of TaSCAMP17 and a recombinant overexpression vector thereof to improvement of plant salt tolerance, the nucleotide sequence of the TaSCAMP17 is shown as SEQ ID NO.1, and the amino acid sequence of the TaSCAMP17 is shown as SEQ ID NO.2. The invention further discloses a preparation method of the TaSCAMP17. TaSCAMP17 overexpression is transformed into arabidopsis thaliana for functional verification, and results show that the salt tolerance of the arabidopsis thaliana overexpressed with TaSCAMP17 is remarkably improved compared with that of wild arabidopsis thaliana, so that TaSCAMP17 plays a positive regulation role in the salt tolerance process of wheat, a theoretical basis and a gene resource are provided for wheat molecular breeding, and a foundation is provided for obtaining salt-tolerant high-yield strains in the future. The method has very important significance in improving the salt tolerance of the wheat.

The application belongs to the technical field of biology and specifically relates to a casein phosphopeptide for promoting calcium, iron and zinc mineral absorption and improving bone health. The application obtains the casein phosphopeptide containing high-activity peptide segments by optimizing the process, and takes six mineral elements of calcium, magnesium, copper, iron, zinc and manganese as research objects. Firstly, the mineral deficiency rat model is used to evaluate the mineral utilization of the CPP. The consumption of 7mg / kg.d increases the contents of Ca, Mg, Cu, Fe, Zn and Mn in femur by 7.12%, 3.56%, 20.76%, 15.84%, 9.94% and 67.12% respectively. Secondly, the molecular docking technology is used to screen the peptide segments with calcium absorption activity from the CPP and perform function verification. The application aims to reveal the effect of the CPP in the mineral absorption and utilization from the cell and animal levels, and provide a theoretical basis for the functional development.

The invention discloses a method for efficiently and directionally screening functional lactic acid bacteria, and the core principle of the method is that a DNA-electrochemical electrode carrying a specific DNA probe is adopted to quickly determine whether a detection sample contains a target strain or not, so that lactic acid bacteria strains with specific functions are efficiently and directionally screened out. The method comprises the following main steps: determining a target strain and a functional gene; designing a primer and preparing a DNA-electrochemical electrode; extracting DNA of a sample, and carrying out ferrocene labeled PCR (Polymerase Chain Reaction) amplification; carrying out electrochemical detection on a PCR product; gradually screening according to an electrochemical detection result; and carrying out identification and functional verification on the screened strains. The method provided by the invention has the advantages of strong pertinence (directional screening), high efficiency, low cost, one-step implementation and the like, and has relatively high development value and wide application prospect.

The invention discloses application of a Homer 1 gene in regulating and controlling the water-holding capacity character of a pig, and belongs to the technical field of biology. Candidate genes related to the water-holding capacity are analyzed and screened through transcriptomics; and identifying proteins and metabolic markers related to the water-holding capacity in the longissimus dorsi aging process by utilizing metabonomics and proteomics technologies. And carrying out cellular level function verification on the screened key gene Homer 1 through multi-omics integration analysis. The result shows that the Homer 1 gene is negatively correlated with the water-holding capacity, so that a theoretical basis is provided for understanding a regulation mechanism of the water-holding capacity of the pig skeletal muscle; meanwhile, candidate gene resources and theoretical support are provided for breeding of high-quality and high-efficiency new pig varieties.

This disclosure provides a method and related products for genome sequence generation and model training. During the training phase, pre-trained multimodal data, including sequence data, functional annotation data, omics data, and species evolution information, is acquired. A fused representation is obtained through heterogeneous coding and multimodal fusion, and input into the sequence generation model for feature calculation, outputting the predicted base probability distribution for each base position to be generated. The model is optimized by combining a multi-task training mode of autoregressive generation and intermediate padding, and a weighted loss function based on biological annotation. During the inference phase, sequences are generated progressively based on multimodal generation context information. Candidate bases are obtained through sampling and evaluated using biological constraints, omics validation, structural validation, and functional validation. Resampling is performed when conditions are not met until preset conditions are satisfied. This method can improve the biological rationality and application value of the generated sequences.

The invention discloses a rape cold-resistant gene and application thereof, and belongs to the technical field of gene engineering. Four rape specific lone genes (BnaOGs) and 2859 brassica specific genes (BSGs) are identified through comparative genomics and multi-database artificial verification by combining UniProt-KB, Nrdb and the like, and the characteristics of short sequence, high GC content, no transcription factor and the like are found. Aiming at a BSG gene BnaA03G0334200ZS, a CDS sequence of the BSG gene BnaA03G0334200ZS is cloned, a pEGOEP35S-H overexpression vector containing a 35S strong promoter and a hygromycin selection marker is constructed, and a positive plant is obtained through agrobacterium-mediated transformation of brassica napus Westar. Functional verification shows that overexpression of the gene remarkably enhances the cold resistance of the rape, plant damage is slight at low temperature, the relative conductivity and MDA content are reduced, and the activities of SOD, POD and CAT are improved. The invention provides a new target and technical support for cultivation of cold-resistant rape varieties, and has a wide application prospect.

This invention discloses a method for wheat grain quality detection and functional gene analysis based on hyperspectral imaging technology, belonging to the field of agricultural testing technology. The method includes: extracting hyperspectral indices; measuring wheat grain quality indicators as manual measurements; assigning weight coefficients to each quality indicator to digitize the wheat quality phenotype; extracting characteristic wavelengths to establish predictive models for each indicator and wheat quality values; performing GWAS on hyperspectral indices and chemical indicators as phenotypic traits with genotypes to screen SNP loci, conduct co-location analysis, obtain candidate genes, and perform functional verification. This invention, through hyperspectral technology combined with chemometric modeling, is suitable for real-time monitoring during wheat quality breeding and processing; by combining spectral phenotype with GWAS gene mining, it overcomes the limitations of traditional quality detection that only serves phenotypic screening, forming a closed loop of "non-destructive detection - gene localization," providing targeted genes for wheat quality improvement.

The invention belongs to the technical field of genetic engineering breeding, and discloses a wheat disease-resistant factor gene TaPsaF and application thereof. A TaPsaF gene sequence is analyzed, and a TaPsaF-GFP fusion protein is constructed, so that the localization of the TaPsaF-GFP fusion protein in cell chloroplast is determined. An overexpression and silence vector of the TaPsaF gene is successfully constructed by using a gene engineering technology, and wheat is transformed by using an agrobacterium-mediated method to obtain a transgenic wheat material. Functional verification shows that over-expression of the TaPsaF gene can significantly enhance the resistance of wheat to stripe rust, and silencing of the gene leads to reduction of the resistance. Gene resources and a technical path are provided for wheat stripe rust resistance molecular breeding, the blank of the TaPsaF protein in biological stress response mechanism research is filled up, and the TaPsaF protein has important significance for guaranteeing food safety.

The invention discloses a compound for treating leukemia. According to the application, the compound 526 with high activity is identified through high-throughput screening and functional verification. The compound 526 can obviously kill AML cells and prolong the lifetime of a patient, is expected to provide a new strategy for overcoming the existing treatment bottleneck, and has a wide application prospect.

The invention belongs to the technical field of gene engineering, and particularly relates to application of CmIAA27 in regulation and control of lateral branch development of chrysanthemum. According to the invention, phenotypic analysis is carried out on a CmCMT2-RNAi transgenic 'Shenma' plant obtained at an earlier stage, and lateral branches of the CmCMT2-RNAi transgenic 'Shenma' are increased in a vegetative growth period. RNA-seq is carried out on the gene CmIAA27, it is found that the expression quantity of the auxin inhibitory signal key gene CmIAA27 is remarkably increased, McrBC and BSP experiments show that the CHH type methylation level on a CmIAA27 promoter in a transgenic line is remarkably lower than that of WT, and the expression of the plant lateral branch development key inhibitory gene CmBRC1 in the transgenic line is remarkably reduced. Y1H, LUC and EMSA experiments show that the CmCMT2 can be directly combined with a CmIAA27 promoter and regulate and control the CHH type methylation level in the region so as to influence the expression of the CmIAA27. Through transcription analysis of CmCMT2-RNAi transgenic Shenma and WT, a key factor CmIAA27 for downstream regulation and control of chrysanthemum lateral branch development is obtained, functional verification is carried out, and excellent gene reserve and a new strategy are provided for regulation and control of chrysanthemum lateral branches.

The application discloses a pichia pastoris bioengineering bacterium for relieving degradation of recombinant collagen, a preparation method and application thereof. Through bioinformatics analysis, gene knockout and function verification screening, three protease genes capable of relieving degradation of collagen in a pichia pastoris host are obtained, namely, chr1-4_0611, chr1-4_0362 and chr2-2_0380. Knocking out the protease chr1-4_0611 can make the total protein yield accumulation increase to 3.5 times of that of a control bacterium, and the proportion of a target band is greater than 50%, which can provide good theoretical and technical guidance for effective expression of other recombinant proteins in the pichia pastoris host.

The invention relates to an application of a corn ZmXTH27 gene in improving the cold resistance of plants. The ZmXTH27 gene encodes a protein of a xyloglucan endotransglycosidase / hydrolase (XTH) family, the gene expression level of the ZmXTH27 gene is remarkably increased under low-temperature stress, and a mutant zmxth27 of the gene shows a low-temperature sensitive phenotype. The ZmXTH27 gene is transformed into arabidopsis thaliana and is subjected to functional verification, and compared with wild type arabidopsis thaliana, the tolerance of an arabidopsis thaliana plant over-expressed with the ZmXTH27 gene to cold stress is remarkably enhanced. According to the invention, more theoretical bases are provided for corn cold resistance research, and gene resources and biotechnological means are provided for subsequent cultivation and germplasm resource innovation of new cold-resistant corn strains.

The invention discloses a non-specific peroxygenase mutant and an application thereof. According to the method, Collariella virescens UPO of which the protein structure is analyzed is rationally designed by utilizing a multiple computer-aided strategy, and through functional verification, a UPO mutant library which has rich enantioselectivity to various substrates and is improved in activity is successfully obtained, so that a solid foundation is laid for researching and developing a green and efficient chiral compound production method. Compared with the prior art, the non-specific peroxygenase mutant provided by the invention shows high catalytic activity in selective oxidation of carbon-hydrogen bonds of various substrates, the selectivity is remarkably improved or overturned, the obtained reaction product is high in optical purity, the reaction efficiency is improved, the separation process is simplified, the cost is reduced, and the non-specific peroxygenase mutant is suitable for industrial production. Good industrial application prospects are realized.