151 results about "Functional validation" patented technology

A method and system for obfuscating sensitive data while preserving data usability. The in-scope data files of an application are identified. The in-scope data files include sensitive data that must be masked to preserve its confidentiality. Data definitions are collected. Primary sensitive data fields are identified. Data names for the primary sensitive data fields are normalized. The primary sensitive data fields are classified according to sensitivity. Appropriate masking methods are selected from a pre-defined set to be applied to each data element based on rules exercised on the data. The data being masked is profiled to detect invalid data. Masking software is developed and input considerations are applied. The selected masking method is executed and operational and functional validation is performed.

Expressed Sequence Tags (ESTs) isolated from maize are disclosed. The ESTs provide a unique molecular tool for the targeting and isolation of novel genes for plant protection and improvement. The disclosed ESTs have utility in the development of new strategies for understanding critical plant developmental and metabolic pathways. The disclosed ESTs have particular utility in isolating genes and promoters, identifying and mapping the genes involved in developmental and metabolic pathways, and determining gene function. Sequence homology analyses using the ESTs provided in the present invention, will result in more efficient gene screening for desirable agronomic traits. An expanding database of these select pieces of the plant genomics puzzle will quickly expand the knowledge necessary for subsequent functional validation, a key limitation in current plant biotechnology efforts.

Expressed Sequence Tags (ESTs) isolated from rice are disclosed. The ESTs provide a unique molecular tool for the targeting and isolation of novel genes for plant protection and improvement. The disclosed ESTs have utility in the development of new strategies for understanding critical plant developmental and metabolic pathways. The disclosed ESTs have particular utility in isolating genes and promoters, identifying and mapping the genes involved in developmental and metabolic pathways, and determining gene function. Sequence homology analyses using the ESTs provided in the present invention, will result in more efficient gene screening for desirable agronomic traits. An expanding database of these select pieces of the plant genomics puzzle will quickly expand the knowledge necessary for subsequent functional validation, a key limitation in current plant biotechnology efforts.

Expressed Sequence Tags (ESTs) isolated from soybean are disclosed. The ESTs provide a unique molecular tool for the targeting and isolation of novel genes for plant protection and improvement. The disclosed ESTs have utility in the development of new strategies for understanding critical plant developmental and metabolic pathways. The disclosed ESTs have particular utility in isolating genes and promoters, identifying and mapping the genes involved in developmental and metabolic pathways, and determining gene function. Sequence homology analyses using the ESTs provided in the present invention, will result in more efficient gene screening for desirable agronomic traits. An expanding database of these select pieces of the plant genomics puzzle will quickly expand the knowledge necessary for subsequent functional validation, a key limitation in current plant biotechnology efforts.

The invention relates to the technical field of plant genetic engineering, and more particularly relates to separated clone and functional verification of DNA segments of paddy disease-resistant related gene OsEDR1. By utilizing transgenic technology based on RNA interference (RNAi) principle, part of the DNA segments of the OsEDR1 gene are connected with a vector expressing double-strand RNA to be converted into rice varieties, and the expression of the OsEDR1 gene in the rice varieties can be inhibited. Genetic transformation rice with obviously reduced OsEDR1 gene expression quantity has the remarkably improved resistibility for bacterial blight and bacterial streak disease, so as to prove that the OsEDR1 gene is negative regulatory factor in the reaction of resisting bacterial blight and bacterial streak disease of rice, thus the disease resistance of the rice can be improved by inhibiting the expression of the OsEDR1 gene.

The invention relates to the technical field of rice gene engineering. Application of miR164 small RNA of rice for controlling development and fertility of a root system of a plant to genetic improvement of the rice is obtained through separation, cloning and functional verification, and the gene is one of the following nucleotide sequences: a DNA sequence shown in a sequence table SEQ NO:1 and an RNA sequence shown in SEQ NO:2. After connecting with an exogenous promoter, a nucleotide sequence containing miR164 precursor is transferred into the rice, the root system of the transgenic rice is developed and sterile, and the fertility can be recovered by externally applying hormone.

Expressed Sequence Tags (ESTs) isolated from maize are disclosed. The ESTs provide a unique molecular tool for the targeting and isolation of novel genes for plant protection and improvement. The disclosed ESTs have utility in the development of new strategies for understanding critical plant developmental and metabolic pathways. The disclosed ESTs have particular utility in isolating genes and promoters, identifying and mapping the genes involved in developmental and metabolic pathways, and determining gene function. Sequence homology analyses using the ESTs provided in the present invention, will result in more efficient gene screening for desirable agronomic traits. An expanding database of these select pieces of the plant genomics puzzle will quickly expand the knowledge necessary for subsequent functional validation, a key limitation in current plant biotechnology efforts.

Expressed Sequence Tags (ESTs) isolated from cotton are disclosed. The ESTs provide a unique molecular tool for the targeting and isolation of novel genes for plant protection and improvement. The disclosed ESTs have utility in the development of new strategies for understanding critical plant developmental and metabolic pathways. The disclosed ESTs have particular utility in isolating genes and promoters, identifying and mapping the genes involved in developmental and metabolic pathways, and determining gene function. Sequence homology analyses using the ESTs provided in the present invention, will result in more efficient gene screening for desirable agronomic traits. An expanding database of these select pieces of the plant genomics puzzle will quickly expand the knowledge necessary for subsequent functional validation, a key limitation in current plant biotechnology efforts.

The invention relates to the technical field of plant gene engineering, in particular to the isolation cloning and function verification of a DNA fragment containing a rice disease resistance related gene OsWRKY45-1. A nucleotide sequence of the OsWRKY45-1 gene and an amino acid sequence encoded by the gene are shown in SEQ ID NO: 1. Genetically transformed rice of which the expression level of the OsWRKY45-1 gene is remarkably reduced apparently strengthens the resistance on xanthomonas oryzae pv. oryzae and xanthomonas oryzae pv.oryzicola, which proves that the OsWRKY45-1 gene is a negative regulatory factor in reactions of resisting bacterial blight and bacterial stripe of rice, and the disease resistance of the rice can be improved by inhibiting the expression of the OsWRKY45-1 gene.

Expressed Sequence Tags (ESTs) isolated from cotton are disclosed. The ESTs provide a unique molecular tool for the targeting and isolation of novel genes for plant protection and improvement. The disclosed ESTs have utility in the development of new strategies for understanding critical plant developmental and metabolic pathways. The disclosed ESTs have particular utility in isolating genes and promoters, identifying and mapping the genes involved in developmental and metabolic pathways, and determining gene function. Sequence homology analyses using the ESTs provided in the present invention, will result in more efficient gene screening for desirable agronomic traits. An expanding database of these select pieces of the plant genomics puzzle will quickly expand the knowledge necessary for subsequent functional validation, a key limitation in current plant biotechnology efforts.

Expressed Sequence Tags (ESTs) isolated from soybean are disclosed. The ESTs provide a unique molecular tool for the targeting and isolation of novel genes for plant protection and improvement. The disclosed ESTs have utility in the development of new strategies for understanding critical plant developmental and metabolic pathways. The disclosed ESTs have particular utility in isolating genes and promoters, identifying and mapping the genes involved in developmental and metabolic pathways, and determining gene function. Sequence homology analyses using the ESTs provided in the present invention, will result in more efficient gene screening for desirable agronomic traits. An expanding database of these select pieces of the plant genomics puzzle will quickly expand the knowledge necessary for subsequent functional validation, a key limitation in current plant biotechnology efforts.

Methods for correlating genes and gene function are provided. Such methods generally involve selecting a candidate gene that appears to be correlated with a particular cellular state or activity and then validating the role of the candidate gene in establishment of such a cellular state or activity. Certain methods utilize RNA interference techniques in the validation process.

Expressed Sequence Tags (ESTs) isolated from soybean are disclosed. The ESTs provide a unique molecular tool for the targeting and isolation of novel genes for plant protection and improvement. The disclosed ESTs have utility in the development of new strategies for understanding critical plant developmental and metabolic pathways. The disclosed ESTs have particular utility in isolating genes and promoters, identifying and mapping the genes involved in developmental and metabolic pathways, and determining gene function. Sequence homology analyses using the ESTs provided in the present invention, will result in more efficient gene screening for desirable agronomic traits. An expanding database of these select pieces of the plant genomics puzzle will quickly expand the knowledge necessary for subsequent functional validation, a key limitation in current plant biotechnology efforts.

The invention provides a cloning and transient expression method of persimmon deastringency related genes. The method comprises: first acquiring gene 3'-terminal sequences, then employing 3' RACE (rapid amplication of cDNA ends) technology to acquire 3'-terminal sequences of ADH and PDC gene family members in persimmon fruits; adopting Q-PCR (quantitative polymerase chain reaction) technology to analyze the genetic expression of ADH and PDC gene family members in a persimmon fruit deastringency treatment; and conducting separation to obtain full-length sequences of ADH and PDC gene family members, then establishing a transient expression system of persimmon leaves. The method of the invention clones the persimmon deastringency related gene family members, analyze the expression patterns of the family members in the persimmon deastringency treatment, and finally verifies functions of the target genes through transient expression of the persimmon leaves. The invention clarifies the regulation mechanism for postharvest persimmon deastringency from the molecular mechanism aspective, and can be used for further functional verification (transgenosis, gene interaction and the like) study of the postharvest persimmon deastringency mechanism.

The invention discloses a script-based and module drive-based method for organizing code-level network protocol simulation verification, which pertains to the research field of network protocol simulation verification; the method is characterized by comprising the following steps that: the function of a tested protocol or codes of the core algorithm are registered at a verification client end and then compiled and debugged; next, a simulation verification request is sent to a verification server end according to commands of users; the verification server inquires a simulation item list according to the request and sends a corresponding script to the verification client end; after the receipt of the script, the verification client end and the verification server end respectively conduct simulation interaction step by step according to the simulation script; and finally, the function verification of the tested code is finished. The method realizes the fast and effective verification to the function of the new developed protocol and the code function of the core algorithm .

The invention discloses a pear hexokinase gene PbHXK1 and an application thereof. A nucleotide sequence of the gene is shown as a sequence table SEQ ID No.1, wherein an amino acid sequence corresponding to the nucleotide sequence of the gene is shown as a sequence table SEQ ID No.2. The gene PbHXK1 is inoculated into tomato to carry out functional verification; the expression quantity of the gene PbHXK1 and the activity of the hexokinase of a transgenosis tomato plant which is obtained by taking a wild tomato plant as a reference are obviously improved, the growth of the plant is obviously inhibited and the content of soluble sugar is obviously reduced so as to show that the cloned gene PbHXK1 is a functional structure gene for coding the hexokinase, has the function of phosphorylating hexose, plays a regulation role in the fruit sugar accumulation process and also takes part in regulation of the growth and the development of the plant.

The invention discloses a DNA encoded compound screening method for an identification tag-free protein or cell lysate with an antibody. The method includes the steps of: (1) coating dynabead protein Gwith the antibody; (2) adding a solution containing the target protein into the antibody combined magnetic beads, and washing off the substances that do not combine with the antibody; (3) adding a DNA encoded compound library for screening; (4) cleaning and removing the uncombined compound; (5) extracting the uncombined compound by heating; and (6) decoding the compound structure by next-generation sequencing, and conducting synthesis and subsequent functional verification. The method provided by the invention avoids the tedious process of protein purification, at the same time solves the problem that the purified protein does not contain post-translational modification, and can effectively improve the screening efficiency and reduce the screening cost.

The disclosed method discloses embedded type software application architecture (ETSAA) oriented to instrument equipment in multimedia category. The method is established based on a set of embedded type software application component library oriented to digitalized instrument equipment in multimedia category, especially a multimedia dedicated component library. The invention realizes service function flow of multimedia application system, and provides audio and video codec with powerful functions. In the method, a platform installation of embedded system of instrument equipment in multimedia category provides tools and environment support for fast developing. Since the said ETSAA oriented to instrument equipment in multimedia category is passed the functional validation, the invention shortens development cycle greatly, and reaches standardization, modularization, and reusability of system software.

The invention provides a root-specific and harm inducible promoter GmChi1p separated from glycine max. A nucleotide sequence is shown as SEQ ID NO.1. A glycine max chitinase 1 gene promoter (GmChi1p) is cloned, and a plant expression vector pCAM1391Z-GmChi1p is constructed by a primer designed uniquely and taking deoxyribonucleic acid (DNA) of a glycine max 'Jungery' genome as a template; and functional verification is performed by bacillus-mediated tobacco genetic transformation and a mode of expressing a glucuronidase (GUS) gene, namely the promoter can drive the GUS gene to be expressed specifically in roots of transgenic tobaccos and to be subjected to inducible expression efficiently by harm in the transgenic tobaccos. The promoter can replace a 35S promoter to be applied to the study of transgenic plants, and particularly the study of the pest and disease resistance, adverse resistance, high yield or quality improvement of transgenic crops.

Expressed Sequence Tags (ESTs) isolated from cotton are disclosed. The ESTs provide a unique molecular tool for the targeting and isolation of novel genes for plant protection and improvement. The disclosed ESTs have utility in the development of new strategies for understanding critical plant developmental and metabolic pathways. The disclosed ESTs have particular utility in isolating genes and promoters, identifying and mapping the genes involved in developmental and metabolic pathways, and determining gene function. Sequence homology analyses using the ESTs provided in the present invention, will result in more efficient gene screening for desirable agronomic traits. An expanding database of these select pieces of the plant genomics puzzle will quickly expand the knowledge necessary for subsequent functional validation, a key limitation in current plant biotechnology efforts.

The invention discloses a transcription factor relevant to blossoming of gossypium aridum, namely gossypium wild species gossypium aridum WRKY gene GarWRKY9, which is characterized in that the transcription factor has a sequence as SEQ ID NO. 2 (Sequence Identifier Number 2) shown in a sequence table. The protein GarWRKY9 relevant to the blossoming of gossypium aridum is separated by using electronic cloning and RT-PCR (Reverse Transcription-Polymerase Chain Reaction) technologies, functional verification is conducted by transgenic arabidopsis, and the transcription factor is proven to be able to advance the blossoming of a plant.

The invention provides SSR molecular markers for identifying resistance of apple on the glomerella leaf spot. The molecular markers are S0607039, S0607001, S0506206, S0506078, S0506001, S0405195, S0405127, S0304673 and S0304011 respectively. The invention further provides primers used for multiplying the SSR molecular markers for identifying the resistance of apple on the glomerella leaf spot. The genes, resistant to glomerella leaf spot, of the apple are subjected to molecular marking through the self-designed SSR markers, so that the position of chromosomes and the genetic distance can be determined, and a tightly linked genetic map is established. The molecular marker closest to the resistance gene can be used for accurately identifying the resistance of apple on the glomerella leaf spot, so that an effective tool is provided for apple production practice and resistance breeding, and a good basis is laid for next cloning and functional verification of the resistance gene.

Expressed Sequence Tags (ESTs) isolated from maize are disclosed. The ESTs provide a unique molecular tool for the targeting and isolation of novel genes for plant protection and improvement. The disclosed ESTs have utility in the development of new strategies for understanding critical plant developmental and metabolic pathways. The disclosed ESTs have particular utility in isolating genes and promoters, identifying and mapping the genes involved in developmental and metabolic pathways, and determining gene function. Sequence homology analyses using the ESTs provided in the present invention, will result in more efficient gene screening for desirable agronomic traits. An expanding database of these select pieces of the plant genomics puzzle will quickly expand the knowledge necessary for subsequent functional validation, a key limitation in current plant biotechnology efforts.

The invention provides a method for rapidly screening a bacterial quorum sensing inhibitor (QSI) by utilizing a suicide gene. The method is characterized in that under natural light, bacteria not infected with a phage do not turn blue and bacteria infected with the phage turn blue; induced by an added lethal condition inducer, a specific polypeptide with the function of inhibiting QS does not start expression of the suicide gene because of inhibiting the activities of QS molecules, so the bacteria survive; a non-specific polypeptide can not inhibit the QS molecules, so the suicide gene starts lethal; then survival candidate clones are picked out, functional verification is further carried out and finally a DNA (deoxyribonucleic acid) sequence corresponding to a specific polypeptide sequence is obtained by a method of DNA sequencing, thus obtaining an efficient and specific QSI polypeptide. The method is simple in steps, and multiple QSI candidate polypeptides can be obtained only within three days to about one weak, so that the method has very obvious advantages.

The invention belongs to the technical field of rice genetic engineering, and in particular relates to gene OsHSF08 capable of remarkably increasing drought resistance during seeding stage and booting stage, which is obtained through screening, separating, cloning and functional verification. The gene OsHSF08 capable of controlling rice drought resistance character is obtained through cloning by screening insertion of T-DNA into rice mutant library, and has nucleotide sequence shown in SEQ ID No: 1; and the coded protein has nucleotide sequence shown in SEQ ID No: 2. Coseparation detection shows that the mutant is tightly linked with drought sensitivity phenotype, and the over-expression of OsHSF08 gene in rice can remarkably increase the drought resistance of the genetically modified rice, so that new function and new application approach of the gene are proven.

The invention belongs to the field of plant biotechnology. The invention specifically involves the separation and cloning of a DNA fragment of rice anti-diseases related gene OsDR6 and functional verification. Combining part of the DNA fragment of OsDR6 gene with vectors able to express double-stranded RNA and transforming into diseased rice varieties using transgenic technology based on RNA interference (RNAi) principle to inhibit the expression of OsDR6 in diseased rice varieties. Resistance of the genetic transformed rice with OsDR6 gene expression significantly reducing against prostheca blight bacteria increases apparently, proving that gene OsDR6 is negative regulation factor in bacterial leaf blight resistant reaction of the rice. The disease-resistant capacity of the rice can be enhanced by inhibiting its function.

The invention relates to the technical field of rice gene engineering. An application of OsSRO1c rice for raising drought durability to genetic improvement of rice drought resistance is obtained through separation, cloning and functional verification. OsSRO1c gene is cloned by using a method of sieving a T-DNA inserted rice mutant library, an expression level detection and an drought stress phenotype identification indicate that the mutant is coseparated with a drought sensitive phenotype; overexpression of OsSRO1c gene can enhance the drought durability of transgenic rice, thereby the functions and application approaches of the gene can be verified.

A system, apparatus, computer program product and method of performing functional validation testing in a system are provided. Generally, functional validation testing includes data acquisition and data validation testing. During the functional validation testing two devices may be exchanging data. The exchange of data by the two devices may be referred to as data acquisition. The data from one device and the data from the other device may be compared to each other. This may be referred to as data validation. When data is exchanged during data acquisition, it is also stored in appropriate locations in a pool of buffers in memory. During the data acquisition, checks are made to determine if the system is entering an idle cycle. If so, the data validation test is performed by using the data in the pool of buffers in memory.

The invention belongs to the technical field of genes and especially relates to application of a gene Loc_Os01g12810. The gene Loc_Os01g12810 can be applied to rice chloroplast development regulationmechanism researches; a gene method is utilized to regulate an expression level of the gene Loc_Os01g12810 to research rice chloroplast development regulation mechanisms; a nucleotide sequence of thegene Loc_Os01g12810 is shown in SEQ ID NO:1 in a sequence table; an amino acid sequence of encoding protein of the gene Loc_Os01g12810 is shown in SEQ ID NO:2 in the sequence table. According to the application of the gene Loc_Os01g12810 disclosed by the invention, that the rice gene Loc_Os01g12810 is a gene related with rice chloroplast development is proved for the first time. The Loc_Os01g12810is a PPR gene. Cloning and biological function verification of the gene have important reference significance in rice chloroplast development molecule mechanism researches and researches on effects of PPR family gene in rice development.

The present invention provides Homo sapiens Recon 1, a manually assembled, functionally validated, bottom-up reconstruction of human metabolism. Recon 1's 1496 genes, 2004 proteins, 2766 metabolites, and 3311 biochemical and transport reactions were extracted from more than 50 years of legacy biochemical knowledge and Build 35 of the human genome sequence.