26 results about "Spirulina sp." patented technology

The application relates to a method for extracting ergothioneine and selenoprotein from spirulina, wherein after culturing FACHB-Se1500 strains under the above-mentioned nitrogen deficiency induction condition, a product is obtained, and after purification, selenoprotein containing ergothioneine is obtained; the FACHB-Se1500 strains are cultured in a Zarrouk+Se culture medium for 10 days, and the strain culture conditions are as follows: 25-30 DEG C, pH=9.5, and light intensity is 40 muE.m-2.s-1 ‑2 ·S ‑1 After reaching the plateau, the strains are respectively transferred to a Zarrouk-N, a Zarrouk-Fe and a Zarrouk-Mg culture medium for induction culture for 2 days, and after collecting the algal powder, selenoprotein containing ergothioneine is obtained after purification. The application further screens a spirulina mutant strain with high selenium tolerance, wherein the algal strain tolerating 800 mg / L Na2SeO3 has the best salt tolerance and can tolerate 1000 mM NaCl; and it is found again through the nitrogen deficiency induction that the ergothioneine content can reach 103.65 mu g / g.

The application discloses a method for preparing goldfish feed from fermented abalone viscera powder, and comprises the following steps: preparing fermented abalone viscera powder, including raw material pretreatment, strain activation, anaerobic fermentation and final treatment; preparing a formula of the goldfish feed, including fermented abalone viscera powder, spirulina powder, fish meal, soybean meal, rice bran, wheat flour, fish oil, alpha-starch, soybean lecithin, calcium dihydrogen phosphate, vitamin premix, mineral premix, betaine, garlicin, VC-phosphate, lysine and choline chloride; and preparing the goldfish feed, including accurate batching, uniform mixing, moisture adjustment and molding, grading granulation, drying and cooling and grading screening. The fermentation and feed preparation process is simplified, the complicated enzymolysis-fermentation combined process is abandoned, no professional technical personnel are needed, and the household breeders can prepare the feed through simple equipment, and the enterprises can mass-produce the feed; the formula is optimized for goldfishes in different growth stages, is suitable for multiple goldfishes such as golden goldfish and grass goldfish, and has high application flexibility.

This invention relates to a method for the targeted cultivation of live feed to enhance the stress resistance of grouper fry, specifically a method for the targeted cultivation of grouper fry and its live feed. The live feed includes artichoke culture and rotifer culture. The targeted cultivation of the artichoke culture includes the following steps: placing artichokes in seawater, adding *Cryptospira spp.* powder, *Spirulina* powder, arginine, and *Cinnamomum camphora* oil for cultivation to obtain the artichoke culture; the targeted cultivation of the rotifer culture includes the following steps: placing rotifers in seawater, adding *Nyctaginus* pink bacteria. Natrialba pink Rotifer cultures were obtained by cultivating bacterial powder, spirulina powder, methionine, and sunflower oil. This method, through targeted cultivation of live feed, enriches the live feed with corresponding functional components. After feeding it to newly hatched juvenile scorpion, it can significantly improve the stress resistance of the juveniles and increase their survival rate, thus solving the industry pain points of slow development, low survival rate, and poor stress resistance of newly hatched scorpion scorpion.

This invention discloses a method for cultivating Spirulina, relating to the field of microalgae biotechnology, aiming to overcome the shortcomings of existing technologies in terms of low Spirulina biomass yield and protein content. The method includes: in a first cultivation stage, inoculating Spirulina into a basal culture medium and conducting photoautotrophic cultivation at a light intensity of 5000-10000 lux until the optical density (OD560) reaches 0.6-0.8; in a second cultivation stage, reducing the light intensity to 1000-3000 lux and adding calcium alginate-encapsulated sodium bicarbonate microspheres, cultivating until the algal cell dry weight reaches 1.5-2.0 g / L; in a third cultivation stage, increasing the light intensity to 8000-12000 lux and adding sodium nitrate slow-release granules supported on anion exchange resin. This invention can achieve a synergistic increase in Spirulina biomass dry weight and protein content.

This invention belongs to the field of algae preservation technology, specifically relating to a chitosan-based nano-preservative for spirulina preservation and its preparation method. The preparation method of the chitosan-based nano-preservative includes the following steps: (1) adding eugenol and zinc acetate sequentially to a tannic acid solution, mixing well, and then ultrasonically treating at 35-45℃ for 2-6 hours to obtain a nanosphere system; (2) adding the nanosphere system obtained in step (1) to a chitosan solution, then adding zinc acetate to the chitosan solution, and stirring and reacting at 25-60℃ for 8-12 hours to obtain the chitosan-based nano-preservative. This invention achieves a multi-effect combination of "antibacterial-antioxidant-slow release" through the synergistic effect of the antibacterial action of eugenol, the antioxidant / anti-inflammatory action of tannic acid, and the auxiliary antibacterial and cross-linking action of zinc acetate, thus solving the problem of the functional limitations of single preservation technology.

This invention discloses a method for improving the emulsifying ability of Spirulina macrophylla protein. The method involves mixing Spirulina macrophylla protein with deionized water to form a dispersion, fully hydrating it at 4°C for 12 hours, centrifuging for 30 minutes to remove insoluble matter, and then sonicating to obtain a modified Spirulina macrophylla protein solution. The surface tension of this modified Spirulina macrophylla protein solution decreases to 9.33-10.06 mN / m, and after 6 hours of static storage, the average particle size of the emulsion decreases to 4.21-6.00 μm, demonstrating significantly better emulsifying performance than the unmodified protein. This method for improving the emulsifying ability of Spirulina macrophylla protein effectively enhances its interfacial activity and emulsifying properties, expanding its applicability and making it widely applicable in the food industry and pharmaceutical functionalization fields.

The present invention relates to a functional salt containing beta-carotene, phycocyanin and carrageenan, and a method for preparing the functional salt. More specifically, the present invention relates to: a functional salt containing, as a functional ingredient, a Dunaliella culture cultured in high-salinity salty water or beta-carotene contained therein, phycocyanin isolated from a Spirulina culture, and carrageenan isolated from an extract of red algae; and a method for preparing the functional salt. The functional salt according to the present invention is an aqueous or powder-type functional salt, and contains beta-carotene, which is a functional ingredient derived from Dunaliella, phycocyanin, which is a functional ingredient derived from Spirulina, and carrageenan, which is a functional ingredient derived from red algae, and thus is beneficial to health, and, has low sodium content, and thus is a low sodium salt, thereby enabling side effects caused by excessive intake of salt to be prevented.

This invention specifically relates to spirulina immunomodulatory peptides, their preparation methods, and applications, belonging to the field of protein bioactive peptide technology. This invention is the first to screen immunomodulatory peptides Ser-Pro-Ser-Trp-Tyr (SPSWY), Met-Phe-Asp-Ala-Phe (MFDAF), and Phe-Gly-Arg-Phe-Arg (FGRFR) from spirulina phycocyanin. The structure and immunomodulatory activity of peptides SPSWY, MFDAF, and FGRFR were clarified. Peptides SPSWY, MFDAF, and FGRFR can enhance macrophage cell viability and phagocytic capacity, and also increase the secretion of NO and cytokines by macrophages. Their immunomodulatory activity also exhibits good stability. Furthermore, peptides SPSWY, MFDAF, and FGRFR have the advantages of being safe, non-toxic, and having high biological activity.

The present application belongs to the technical field of aquatic feed, and discloses a special feed for Eriocheir sinensis breeding and a preparation method thereof. The raw materials of the feed include fish meal, soybean meal, corn meal, wheat meal, shrimp meal, spirulina powder, black soldier fly larvae powder, yeast powder, bile acid, chitosan, calcium dihydrogen phosphate, choline chloride, vitamin C ester, ferrous sulfate and zinc sulfate. The preparation method includes key steps such as raw material ultrafine grinding, grading mixing, liquid conditioning, ring die granulation, post-curing drying and surface spraying of flavor attractant. The present application realizes the synergistic effect among components through the precise compounding of various conventional and functional raw materials and the targeted processing technology. When the feed is applied to the breeding of Eriocheir sinensis, the growth rate, feed conversion rate, immunity and food quality of the finished crab can be significantly improved, and the water pollution in the breeding process can be effectively reduced, so that the breeding goal of health, high efficiency and ecology is achieved.

A method of processing microalgae comprising: adding an inert abrasive agent to a microalgae biomass; and agitating the mixture to lyse the microalgal cells. The abrasive agent may be diatomaceous earth, diatomite, celite, kieselguhr. The microalgae may have not been previously frozen. The microalgae may be of the Spirulina genus (Arthrospira platensis). The method may further comprise separating water-soluble compounds from the mixture. The mixture may be dried to form a cake and contacted with a liquid such as water to leach water-soluble components into the liquid. The liquid may comprise phycocyanin. The liquid may be water. The liquid may be filtered. The method may comprise further steps of removing bacteria, concentrating or purifying the phycocyanin, and spray-dying the phycocyanin solution. The method may comprise a further step of separating the microalgae from the abrasive agent. A preservative may be added to the microalgal contents. Also claimed are compositions comprising microalgal cellular contents. The composition may comprise phycocyanin. Also claimed are uses of the composition as a pigment, colourant, biostimulant or fertiliser. [Fig. 4]

The utility model relates to the technical field of spirulina cultivation, specifically a flow mixing device for spirulina cultivation, including two vertical boards one side all are set up with the sliding slot, the pivot is located between two vertical boards, two adjusting mechanisms are respectively slidably connected in two sliding slot interiors, the adjusting mechanism includes the sliding block, and the sliding block one side is set up with the sleeve hole, the pivot outside wall rotation sleeve is connected in the sleeve hole interior. In the utility model, through starting drive motor can drive the pivot rotation, make the pivot drive multiple agitator plate rotation agitates the water flow, and start electric push rod drives the pivot reciprocating movement along the sliding slot, make each time agitator plate agitates the water flow depth is not one, thereby produces more complex water flow mode, improves the mixing effect of culture solution, and the agitator plate can produce the collision when passing through the knocking rod and relies on the spring to produce the shake, and the adhered algal body is shaken together with the water flow and separates from the agitator plate, thereby reduces the algal body that agitator plate sticks.

This invention relates to a composition for preventing colitis-associated colorectal cancer and controlling weight, comprising seahorse, laver, kelp, spirulina, Ulva procumbens, and Gracilaria in a specific ratio. This formula is based on traditional Chinese medicine theories such as "five colors entering the five internal organs," "five flavors entering the meridians," and "salt can soften hardness," while also considering the combined use of warming and cooling properties, and the integration of clearing and tonifying. Through the combination of various marine algae and seahorse, it achieves synergistic effects on multiple targets, including immune regulation, anti-inflammation, intestinal barrier repair, and weight control. The preparation method is simple and low-cost, suitable for large-scale production in food and dietary supplements. Animal experiments show that this composition can significantly slow weight gain in normal mice. In an AOM / DSS-induced colitis-associated colorectal cancer model, it can reduce the disease activity index, decrease the number of colon tumors, alleviate abnormal organ enlargement and intestinal pathological damage, downregulate inflammatory factors such as iNOS and Cox-2, and upregulate intestinal barrier-related factors such as Cdh1, demonstrating a good preventive intervention effect.

This invention discloses a method for breeding and screening Spirulina strains with high antioxidant activity, relating to the field of microalgae biotechnology. The method involves culturing different candidate Spirulina strains in multiple culture units, installing multispectral sensor arrays in each culture unit to collect real-time spectral data and environmental parameter data during the Spirulina growth process, and obtaining spectral feature vectors related to antioxidant activity. This method enables independent identification and comparative analysis of the optimal activity state of each strain, effectively avoiding missing the activity peak window, significantly improving the screening efficiency and stability of superior high antioxidant activity strains, and providing reliable technical support for the targeted breeding and large-scale application of Spirulina strains.

The present application belongs to the technical field of red claw crayfish breeding, and particularly relates to a fermented feed for improving the quality and immunity of red claw crayfish and a preparation method thereof. The fermented feed for improving the quality and immunity of red claw crayfish is prepared by fermenting the following raw materials in parts by weight: 15-25 parts of imported fish meal, 12-20 parts of fermented soybean meal, 5-8 parts of shrimp shell powder, 3-6 parts of spirulina powder, 5-10 parts of corn gluten meal, 10-15 parts of high-gluten flour, 3-5 parts of yeast extract, 2-4 parts of soybean phospholipid, 3-5 parts of fish oil, 1-2 parts of calcium dihydrogen phosphate, 1-2 parts of multi-vitamin and multi-mineral, 0.1-0.3 parts of beta-glucan, 2-5 parts of compound probiotic fermentation agent, and 32-40 parts of water. The compound probiotic fermentation agent is prepared by mixing Haematococcus pluvialis, Bacillus circulans and marine Rhodotorula in a mass ratio of 3-5:3-4:2-3. The fermented feed of the present application can significantly improve the body color, muscle quality and immunity of the crayfish.

The application discloses an intelligent active composite film with fresh-keeping and monitoring functions and a preparation method and application thereof. The preparation method comprises the following steps: limiting and intercepting thymol by spirulina to obtain spirulina loaded with thymol; coating the spirulina loaded with thymol by carboxymethyl chitosan to prepare an intelligent fragrance micro-reactor with pH / humidity dual response characteristics; and performing film forming treatment on a film forming liquid containing konjac glucomannan, blueberry anthocyanin, the intelligent fragrance micro-reactor and water to prepare the intelligent active composite film with the fresh-keeping and monitoring functions. The intelligent active composite film can realize fresh-keeping through active anti-corrosion intervention, has the in-situ freshness real-time diagnosis function and has a good application prospect in novel green food packaging materials.

The present invention relates to a composition for the nutrition or drink of a non-human animal comprising the combination of spirulina and Ascophyllum nodosum. It further relates to the use of this composition for improving the zootechnical performance of non-human animals.

The present disclosure addresses the problem of providing technology for preventing or improving aging-related hearing loss. This problem is solved by a composition for preventing or improving aging-related hearing loss, the composition comprising, as an active ingredient, at least one selected from the group consisting of spirulina, phycocyanin, and phycocyanobilin.

The application discloses a kind of green extraction spirulina chlorophyll and improve the method of chlorophyll stability.The application is mixed uniformly with HBD and HBA, deionized water is added, and is stirred by magnetic force under 70~80 DEG C to form colorless transparent clear liquid, obtain NADES extraction fluid;NADES extraction fluid and spirulina powder are mixed, and homogenizer is broken wall, and is extracted by magnetic stirring under 30~70 DEG C, after extraction, freeze centrifugation, and take supernatant, spirulina chlorophyll NADES extraction fluid is obtained.The test proves that spirulina chlorophyll NADES extraction fluid prepared by the above method, the light stability and thermal stability of chlorophyll are obviously improved.The application is first applied to spirulina chlorophyll extraction with cheap and easy to obtain, green and environmental protection NADES, solves the problem that traditional extraction method pollutes environment and harms human health, and also can improve the stability of chlorophyll.

Disclosed herein are edible compositions containing a blue colorant having a phycocyanin content of at least 35 wt.% or at least 40 wt.% and uses thereof. The blue colorant can be derived from a natural source, such as Spirulina ( Spirulina ) or Galdieria sulphuraria ( Galdieria sulphuraria ), and can provide color characteristics similar to those provided by the synthetic blue colorant FD&C Blue No. 1.

This invention discloses an animal feed containing β-glucan and its preparation method, belonging to the field of animal feed technology. This invention utilizes β-glucan to activate innate immunity, post-biotics to train the immune system, and spirulina to provide immunopolysaccharides and phycocyanin. These three components together form a three-dimensional immune-enhancing network, synergistically strengthening immune function. Furthermore, β-glucan can also function as a prebiotic, inhibiting pathogens and strengthening the immune barrier through the addition of post-biotics, while barley grass powder provides dietary fiber, collectively maintaining intestinal flora balance, optimizing intestinal health, and maintaining the integrity of the physical barrier. Based on improved immunity and intestinal health, combined with balanced nutrition, this improves animal growth performance, especially during stressful periods such as weaning, transportation, and feed changes, promoting growth and stress resistance.

The present application relates to a kind of fried mulberry leaf pieces and its preparation method.The preparation method of fried mulberry leaf pieces provided by the present application is that fresh mulberry leaf is first treated with acid aqueous solution containing citric acid and magnesium carbonate by pulse ultrasonic treatment, then soaked with aqueous solution containing spirulina extract and zinc gluconate, then the mulberry leaf treated by the specific pretreatment is subjected to cold fermentation by alternating temperature using lactobacillus rhamnosus and lactobacillus plantarum, and then combined with low-temperature plasma-assisted vacuum frying, which has significant effect of deodorization and green protection, and can significantly reduce the generation of harmful substances such as acrylamide during frying, effectively preserve bioactive substances such as polyphenols and flavonoids.The preparation method of fried mulberry leaf pieces provided by the present application can effectively remove grassy odor while the obtained fried mulberry leaf pieces have the advantages of green color, good flavor, less loss of active substances, less accumulation of harmful substances, low oil content, crisp and not greasy taste, etc.

This invention discloses a spirulina compound compressed candy. Through a basic formula—spirulina, astragalus, poria cocos, and jujube—it forms a synergistic mechanism of action that integrates supplementation, transport, transformation, and harmonization. Then, depending on the target population, rose or polygonatum can be added to achieve precise synergistic effects, addressing the problems of poor absorption, weak efficacy, and strong fishy smell associated with using spirulina alone. The invention also discloses a method for preparing the spirulina compound compressed candy. Low-temperature microwave sterilization ensures a 96% retention rate of phycocyanin, preventing nutrient loss caused by high temperatures. Moisture-controlled and nitrogen-filled packaging results in low product moisture content and resistance to moisture absorption and oxidation, extending the shelf life to 24 months. Controlling the uniform particle size of the raw materials achieves a 99.4% tableting rate, resulting in smooth tablets with stable quality, enhancing product competitiveness and premium pricing.

This application discloses a method for preparing spirulina bioactive peptides based on yeast co-fermentation. It belongs to the field of biotechnology. This invention is the first to disclose the use of *Pichia pastoris* to co-ferment spirulina powder with glucose, successfully preparing spirulina bioactive peptides. Compared with traditional enzymatic hydrolysis, this co-fermentation method significantly increases the yield of bioactive peptides to 5.048% under mild conditions (28℃, 24h), representing a maximum increase of 131.4% compared to traditional enzymatic hydrolysis. The obtained bioactive peptides exhibit excellent efficacy in immunological experiments, significantly reversing immunosuppression-induced inhibition of mouse weight gain, increasing spleen index, and completely restoring serum hemolysin levels, with a dose-dependent effect. This method effectively solves the problems of low yield, demanding conditions, high cost, and easily compromised product activity associated with traditional enzymatic hydrolysis, providing an innovative, economical, and green technical solution for the efficient preparation of highly active spirulina peptides.