15 results about "Phycobiliprotein" patented technology

Disclosed herein is a method for enhancing immune response of a vaccine. The method includes preparing a Phycocyanin compound. The Phycocyanin compound includes at least one of C-phycocyanin (C-PC), Phycoerythrin (PE), Allophycocyanin (APC), phycocyanobilin (PCB), and combinations thereof. The method further includes administering the prepared Phycocyanin along with a vaccine composition to a living body.

The utility model relates to the technical field of phycocyanin powder production equipment, in particular to a phycocyanin powder separation and purification device which comprises a bearing frame, a purification mechanism and a collection mechanism, a fixed shell is fixedly connected to the bearing frame, a liquid outlet hole is formed in the fixed shell, a feeding pipe is fixedly connected to the fixed shell, the purification mechanism is arranged in the fixed shell, and the collection mechanism is arranged in the fixed shell. The collecting mechanism is arranged on the fixing shell. According to the phycocyanin purification device disclosed by the utility model, the purification mechanism is arranged in the fixed shell, an extracting solution is conveniently conveyed into the purification mechanism by virtue of the feeding pipe, the extracting solution is conveniently filtered by virtue of the filter membrane, and a phycocyanin solution is conveniently conveyed into the collection box by virtue of the conveying plate and the guide pipe; according to the phycocyanin filtering and concentrating device, the phycocyanin solution is filtered and concentrated, then a collecting box is used for storing the filtered and concentrated phycocyanin solution, a stepping motor I is started, so that a used filter membrane is convenient to replace, and when a communicating hole in which impurities are stored vertically faces downwards, the impurities conveniently fall into a conveying shell.

The invention discloses a fucoidin-marine mineral composition for promoting skin cell repair and application thereof, and belongs to the technical field of skin care products. The composition provided by the invention is derived from food or small-pollution deep sea, and is high in safety; various mineral substances and active ingredients are contained, so that the nutrition is rich; the components have a synergistic effect, can effectively reduce damage of light damage to cells and promote fibroblasts to secrete collagen, and have good effects on repairing cell damage, delaying skin aging and reducing damage caused by external and internal factors.

The invention discloses phycobiliprotein and / or red algae oligosaccharide extracted based on a biological soluble wall and a preparation method of the phycobiliprotein and / or the red algae oligosaccharide, and belongs to the technical field of biological functional component extraction. The invention provides a preparation method for simultaneously extracting phycobiliprotein and phycobiliprotein of red algae, which is high in efficiency, low in loss rate and suitable for process amplification, specifically, cell wall polysaccharide in the red algae is matched with a compound enzyme in a matching manner, and multiple enzymes are cooperated, so that the contradiction problem between high-temperature dissolution of the cell wall polysaccharide of the red algae and heat sensitivity of phycobiliprotein is effectively solved, and the yield of the phycobiliprotein and the oligosaccharide of the red algae is improved. The wall breaking efficiency is improved, and cell wall breaking is promoted to form porous and macroporous cell wall structures; the outward migration of intracellular substances such as phycobiliprotein is promoted, so that higher extraction efficiency is obtained. According to the preparation method provided by the invention, the extraction amount of phycobiliprotein reaches 224 g / kg after production conversion, and the dissociation loss of phycobiliprotein polymers is effectively reduced. In addition, the phycobiliprotein is efficiently extracted, meanwhile, the red algae oligosaccharide product is obtained, and the process economic benefit and the raw material utilization rate are improved. The preparation method provided by the invention does not need special equipment, is simple and convenient in process, green, environment-friendly, low in cost and beneficial to industrial production.

The object of the invention is a method for extracting, with high yield, phycobiliproteins from cyanobacterial and / or algal biomass, obtaining extracts in water or aqueous solutions characterised by high pigment concentration and a purity degree equal to or greater than the food / cosmetic grade (P≥2). The method is based on a process characterised by a step of breaking down the cyanobacterial / algal cells by ultrasonication in an aqueous solution of ammonium sulphate, and simultaneous extraction of water-soluble compounds other than phycobiliproteins, followed by an extraction step of the phycobiliproteins using water or aqueous solutions.

The invention relates to a porphyra yezoensis extract as well as a preparation method and application thereof, and belongs to the technical field of cosmetics. The preparation method of the porphyra yezoensis extract specifically comprises the steps of homogenate pretreatment, high-temperature swelling, enzyme-assisted extraction, color base removal, solid-liquid separation, concentration, drying and the like, the seaweed structure can be as loose as possible through homogenate pretreatment and high-temperature swelling, and enzymolysis reaction is facilitated when enzyme-assisted extraction and decoloration are performed. Physical and biological methods are comprehensively utilized to remove phycobiliprotein pigment groups, the pigment groups are converted from a chromogenic combined state to a colorless free state, the activity of the phycobiliprotein pigment groups is reserved, interference of the color of the groups is eliminated, blending and application of the phycobiliprotein pigment groups in different cosmetics are facilitated, the subsequent separation process is simple, cost is low, and the phycobiliprotein pigment groups are suitable for industrial production. The industrial production is facilitated.

The application discloses a phycobiliprotein PE-1 with fluorescence characteristics, a fusion protein thereof and application, and belongs to the technical field of genetic engineering. The application provides a novel phycobiliprotein, and the amino acid sequence of the phycobiliprotein is shown as SEQ ID NO. 1. The phycobiliprotein has high fluorescence quantum yield and fluorescence emission capacity. The application further provides a fusion protein constructed by fusing the phycobiliprotein and streptavidin through a connecting peptide, a recombinant expression vector containing the coding sequence of the fusion protein and a recombinant host cell capable of expressing the fusion protein. The fusion protein provided by the application can be used in solid-phase immunodetection systems such as ELISA and immunochromatography, and sensitive detection of target objects can be realized through a fluorescence detection mode of 540 / 580 nm. The fusion protein provided by the application has significant advantages in signal-to-noise ratio and detection stability, and can be widely applied to the fields of biomedical detection and diagnosis.

The invention discloses a DNA (deoxyribonucleic acid) / phycocyanin-polystyrene microsphere fluorescent probe as well as preparation and application thereof. According to the fluorescent probe, polystyrene microspheres serve as a carrier and are covalently coupled with DNA recognition molecules, and phycocyanin is further coupled to the surface of the polystyrene microspheres through covalent bonds to serve as a fluorescent signal unit. The novel fluorescent nanoprobe is successfully constructed by combining the high-brightness fluorescence characteristic of phycocyanin, the carrier effect of polystyrene microspheres and the high-specificity recognition capability of DNA molecules. The probe can be widely applied to high-sensitivity fluorescence detection of targets such as nucleic acid, protein, small molecules and cells, and has a wide application prospect in the fields of biosensing and medical diagnosis.

Methods and systems for extracting phycobiliproteins from seaweed, such as phycoerythrin, are generally described. In some embodiments, the phycobiliprotein extracted from seaweed is phycoerythrin, and the methods and systems described by this disclosure may extract phycoerythrin from seaweed while maintaining other compounds within the seaweed, such as bromoform.

The invention relates to a laver lysing extract as well as a preparation method and application thereof, and belongs to the technical field of biology. According to the method, a strain is obtained from a separating medium in the porphyra yezoensis drying processing process, it is tested that the strain can secrete lysozyme capable of rapidly dissolving porphyra cell walls, the lysozyme comprises amylase and protease, and intracellular phycobiliprotein, low-molecular sulfuric acid galactan and the like can be released after cell dissolution; the purpose that laver cells are lysed and intracellular components are efficiently released is achieved, and the strain is identified as bacillus tequilensis T7. The laver lysate extract prepared by the invention has the effects of resisting oxidation, preventing ultraviolet radiation and the like, and can be applied to the fields of cosmetics, functional foods, beverages and the like.

The invention relates to a phycobiliprotein source xanthine oxidase inhibitory peptide as well as a preparation method and application thereof, and belongs to the technical field of bioactive peptides and functional foods. The phycobiliprotein source xanthine oxidase inhibitory peptide has an amino acid sequence of Ile-Gly-Tyr-Tyr-Leu-Arg or Lu-Asp-Tyr-Tyr-Leu-Arg, and the amino acid sequence table of the phycobiliprotein source xanthine oxidase inhibitory peptide is as shown in SEQ ID NO. 1 or SEQ ID NO. 2. The phycobiliprotein source xanthine oxidase inhibitory peptide provided by the invention is an important protein resource in spirulina and a high-quality source of bioactive peptide, and can be widely applied; the phycobiliprotein source xanthine oxidase inhibitory peptide is low in molecular weight, and compared with a chemically synthesized preparation, food-borne peptides are safer, more efficient, free of toxic and side effects and easy to digest and absorb; the phycobiliprotein source xanthine oxidase inhibitory peptide has high binding affinity with xanthine oxidase, can form a very stable ligand receptor complex, and has excellent inhibition performance on xanthine oxidase.

This invention discloses a large-scale preparation method for high-purity phycobiliproteins, belonging to the field of purification technology for active ingredients in natural products. This invention utilizes the addition of electronegative polysaccharides or oligosaccharides to form a co-soluble solution, which induces phycoerythrin precipitation under low-temperature conditions, achieving effective separation of phycoerythrin and phycocyanin and obtaining high-purity phycoerythrin. This invention, through variable-parameter aqueous two-phase continuous purification, obtains high-purity phycocyanin while effectively removing both existing and added soluble polysaccharides from the phycobiliprotein-containing solution or biomass extract, avoiding interference from the large molecular size and negative charge of polysaccharides on ultrafiltration purification performance. The prepared phycoerythrin has a PE / PC molar ratio >14, and the phycocyanin has a PC / PE molar ratio >4. Compared with traditional purification methods, this invention effectively solves the high cost and complexity of chromatographic preparation of phycobiliproteins. The method for preparing and purifying phycobiliproteins provided by this invention has the advantages of fewer purification steps, high yield, simple process, and easy scalability. Furthermore, the preparation process is green, environmentally friendly, and low-cost, facilitating industrial production and meeting market demand for high-purity phycobiliproteins, thus possessing significant promotional value.

The invention discloses a transgenic synechocystis strain with enhanced tolerance to various heavy metal ions and application of the transgenic synechocystis strain in heavy metal sewage treatment, and belongs to the technical field of sewage treatment. The transgenic synechocystis algal strain is constructed by integrating a superoxide dismutase A (SodA) gene of escherichia coli with a good oxygen stress resistance function into a synechocystis PCC6803 genome. Under the stress condition of heavy metal ions such as Cr < 6 + >, Pb < 2 + > and Cd < 2 + >, the transgenic synechocystis strain shows remarkable heavy metal ion tolerance. Compared with a wild type algal strain, the transgenic synechocystis algal strain has higher activity of superoxide dismutase (SOD) and catalase (CAT), and the content of phycobiliprotein, chlorophyll a and carotenoid is also maintained at a higher level. The invention provides a technical means for application of microalgae in heavy metal sewage treatment and physiological metabolism research.

The present application relates to a kind of extraction process of sea wood ear algae and the application of tough hair quality in hair product, belong to the technical field of extraction of algae.The present application is extracted by water phase preliminary extraction sea wood ear algae, then the mixed solution is centrifuged, filtration, and the supernatant and filter residue are obtained.The supernatant is subjected to ammonium sulfate fractionation precipitation, dialysis desalination, freeze-drying to obtain phycobiliprotein;The filter residue is extracted by supercritical CO2 fluid using lactic acid solution entraining agent, and then concentrated by membrane, dried to obtain sea wood ear polysaccharide.The phycobiliprotein and sea wood ear polysaccharide are fermented by lactobacillus, and the product is rich in lactic acid, amino acid and other small molecule active ingredients, which can penetrate the inside of hair more easily, and has the effect of deep nourishment.

The invention discloses a transgenic synechocystis strain with enhanced stress resistance to various heavy metal ions as well as a construction method and application of the transgenic synechocystis strain, and belongs to the technical field of microorganisms. The transgenic synechocystis algal strain is constructed by integrating a cyanobacteria high expression promoter and a gene HMP3 of a human metallothionein artificial mutant into a synechocystis PCC6803 genome. Detection shows that the transgenic synechocystis strain shows remarkably enhanced stress resistance under the stress condition of heavy metal ions such as Cr < 6 + >, Pb < 2 + > and Cd < 2 + >. Compared with a wild type algal strain, the transgenic synechocystis algal strain has higher activity of superoxide dismutase (SOD) and catalase (CAT), and the content of phycobiliprotein, chlorophyll a and carotenoid is also maintained at a higher level. The invention provides an effective method for constructing a transgenic synechocystis strain resistant to stress of various heavy metal ions, and provides a technical means for application of microalgae in heavy metal sewage treatment.