Bio-modification preparation method for improving activity of laver phycoerythrin

A phycoerythrin and biological technology, which is applied to the preparation methods of peptides, chemical instruments and methods, algae/moss peptides, etc., can solve the problems of poor stability of phycobiliproteins, and achieves improved deep processing value, increased scavenging ability, and improved activity. Effect

Inactive Publication Date: 2010-01-13
NANJING AGRICULTURAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

After modification, these chromophore short peptides not only retain the advantages of the original brig

Method used

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  • Bio-modification preparation method for improving activity of laver phycoerythrin
  • Bio-modification preparation method for improving activity of laver phycoerythrin
  • Bio-modification preparation method for improving activity of laver phycoerythrin

Examples

Experimental program
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Effect test

Embodiment 1

[0023] (1) Weigh 20 grams of laver powder, soak it in 1 mM PBS buffer solution with pH 6.8 at a volume ratio of 1:50 for 24 hours, and fully swell. Cell disruption was performed in an ice bath with an ultrasonic wave of 800W power for 1200s. Centrifuge at 5000rpm for 20min to obtain a crude phycoerythrin solution;

[0024] (2) Filtrate the crude phycoerythrin solution with four layers of gauze, precipitate with ammonium sulfate with a saturation of 20% to 45%, centrifuge (5000rpm, 20min), dissolve the precipitate in 1mM, pH6.8 PBS buffer solution, and run under running water Dialyze for 48 hours, until there is no white precipitate in the 20% barium chloride test, again precipitate with ammonium sulfate with a saturation of 20% to 45%, centrifuge (5000rpm, 20min), and dissolve the precipitate in 1mM, pH6.8 PBS buffer solution, running water dialysis for 48 hours, until 20% barium chloride test without white precipitate, sample freeze-drying after dialysis promptly obtains pha...

Embodiment 2

[0028](1) Weigh 20 grams of laver powder, soak it in 1 mM PBS buffer solution with pH 6.8 at a volume ratio of 1:50 for 24 hours, and fully swell. Cell disruption was performed in an ice bath with an ultrasonic wave of 800W power for 1200s. Centrifuge at 5000rpm for 20min to obtain a crude phycoerythrin solution;

[0029] (2) Precipitate with ammonium sulfate with a saturation of 20% to 45%, centrifuge (5000rpm, 20min), dissolve the precipitate in 1mM, pH6.8 PBS buffer, and dialyze for 48 hours until 20% barium chloride Check that there is no white precipitate, again precipitate with ammonium sulfate with a saturation of 20% to 45%, centrifuge (5000rpm, 20min), dissolve the precipitate in 1mM, pH6.8 PBS buffer, and dialyze with running water for 48 hours until 20 % barium chloride test has no white precipitate, and after dialysis, the sample is freeze-dried to obtain pharmaceutical grade (A 562 / A 280 >2) phycoerythrin, yield 3.12%;

[0030] (3) Dissolve the freeze-dried p...

Embodiment 3

[0033] (1) Weigh 20 grams of laver powder, soak it in 1 mM PBS buffer solution with pH 6.8 at a volume ratio of 1:40 for 24 hours, and fully swell. Cell disruption was performed in an ice bath with an ultrasonic wave of 800W power for 1200s. Centrifuge at 5000rpm for 20min to obtain a crude phycoerythrin solution;

[0034] (2) Precipitate with ammonium sulfate with a saturation of 20% to 45%, centrifuge (5000rpm, 20min), dissolve the precipitate in 1mM, pH6.8 PBS buffer, and dialyze for 48 hours until 20% barium chloride Check that there is no white precipitate, again precipitate with ammonium sulfate with a saturation of 20% to 45%, centrifuge (5000rpm, 20min), dissolve the precipitate in 1mM, pH6.8 PBS buffer, and dialyze with running water for 48 hours until 20 % barium chloride test has no white precipitate, and after dialysis, the sample is freeze-dried to obtain pharmaceutical grade (A 562 / A 280 >2) phycoerythrin, yield 2.97%;

[0035] (3) Dissolve the freeze-dried ...

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Abstract

The invention relates to a bio-modification preparation method for improving activity of laver phycoerythrin, belonging to the technical field of marine organism functional composition preparation. Laver swells after being treated by PBS buffer solution, and crude extract is obtained by using ultrasonic wave cell-break technique to break cell walls; the crude extract is precipitated by ammonium sulfate with saturation of 20-45 percent and filtered to obtain laver phycoerythrin with purity (A562/A280) reaching to 3.04; and the laver phycoerythrin is biologically modified by papain with concentration of 15000-25000mu/g at pH value of 6.5-7.5 and temperature of 50-60 DEG C for two hours. The activity of modified phycoerythrin is remarkably improved, the reducing power (A<700NM>) is increased to 0.573, and the hydroxyl redica removing capability is increased to 51.03 percent, and the reducing power and the hydroxyl redica removing capability are increased by 0.329 and 35.17 percent respectively. In the invention, the method for improving the antioxidant activity of laver phycoerythrin is established, and the bio-modified phycobiliprotein has more application values in medical and food fields.

Description

1. Technical field [0001] The invention relates to a biological modification preparation method for improving the activity of laver phycoerythrin, belongs to the technical field of preparation of marine biological functional components, and is used for research and development of base materials for medical health care products and functional foods. 2. Background technology [0002] Phycobiliprotein is a light-harvesting pigment protein in algal phycobilisomes, which can efficiently transfer the captured light energy to chlorophyll, so that the photosynthesis of algae can occur. Phycobiliproteins mainly include three types of phycocyanin, phycoerythrim and allophycocyanin, among which phycocyanin (PC) and phycoerythrin (PE) are more abundant in algae. Rich. [0003] Phycobiliprotein is a very important raw material for photodynamic drugs. Its unique photosensitizing effect can assist laser cancer treatment, enrich in the lesion, and efficiently generate free radicals and act...

Claims

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Application Information

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IPC IPC(8): C12P21/06C07K14/405C07K1/36C07K1/34C07K1/30
Inventor 胡秋辉赵殿锋杨方美安辛欣辛志宏赵立艳
Owner NANJING AGRICULTURAL UNIVERSITY
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