67results about "Algae/lichens peptides" patented technology

The invention belongs to the technical field of haematococcus separation, and particularly relates to a method for sequentially separating polysaccharide and phycoerythrin from a haematococcus degreased algae residue water extract, and the method comprises the following steps: S1, pretreatment, S2, deep flocculation and primary separation of polysaccharide, and S3, specific precipitation of phycoerythrin. According to the method, a set of sequential separation process is constructed by adopting a self-made hydrophobic flocculant and a composite precipitation inducer, so that a high-purity polysaccharide product and a high-activity phycoerythrin concentrated solution can be synchronously obtained from a haematococcus degreased algae residue water extract. According to the method, deep flocculation of polysaccharide, specific precipitation of phycoerythrin and respective subsequent refining steps are integrated into an ordered whole process, so that the product in the previous step can be directly treated in the next step in an optimal state, and the loss possibly caused by storage and transfer of intermediate materials is reduced; through condition matching and cooperation among the steps, the purity of the final product is improved.

The invention relates to the technical field of plant genetic engineering, in particular to application of an algae NySAT2 gene in regulation and control of alfalfa crude protein and detergent fiber. According to the invention, a sequence after codon optimization of an NySAT2 gene coding region is obtained by using gene synthesis and codon optimization technologies, an algae NySAT2 gene overexpression vector is constructed, a target gene is introduced into an alfalfa genome by using an agrobacterium rhizogenes mediated genetic transformation method, and a transgenic plant is obtained by using a genetic transformation system. Molecular identification and phenotypic analysis show that on the premise of keeping normal growth vigor (plant height and biomass have no significant difference from those of a wild type), the crude protein content of a medicago sativa plant with the overexpressed NySAT2 gene is remarkably increased compared with that of the wild type medicago sativa plant, and meanwhile, the contents of acid detergent fibers and neutral detergent fibers are remarkably reduced, so that the content of the crude protein in the medicago sativa plant with the overexpressed NySAT2 gene is remarkably increased. The forage quality and the feeding value of the medicago sativa are effectively improved.

Methods and kits are provided for producing Griffithsin. The methods include providing a genetically modified microorganism comprising a gene encoding Griffithsin protein operably linked to an inducible promotor and growing the genetically modified microorganism under conditions that induce the promotor and cause expression of griffithisin. The Griffithsin is purified by releasing Griffithsin from the microorganism by cellular disruption, performing a precipitation step to remove contaminating protein and nucleic acids, and performing an anion exchange chromatography step.

Disclosed herein is a method for enhancing immune response of a vaccine. The method includes preparing a Phycocyanin compound. The Phycocyanin compound includes at least one of C-phycocyanin (C-PC), Phycoerythrin (PE), Allophycocyanin (APC), phycocyanobilin (PCB), and combinations thereof. The method further includes administering the prepared Phycocyanin along with a vaccine composition to a living body.

Mutant photosynthetic organisms having reduced chlorophyll and increased photosynthetic efficiency are provided. The mutant strains have mutated or attenuated: chloroplastic SRP54 gene and SGIl gene; chloroplastic SRP54 gene and SGI2 gene; chloroplastic SRP54 gene, SGIl, and SGI2 genes are disclosed. The mutant photosynthetic organisms exhibit increased productivity with respect to wild-type strains. Also provided are mutant photosynthetic organisms having mutated or attenuated cytosolic SRP54 genes. Provided herein are methods of producing biomass and other products such as lipids using strains having mutations in an SRP54 gene, SGIl, SGI2 genes, a combination of SGI1 / SRP54, and a combination of SGI2 and SRP54 genes. Also included are constructs and methods for attenuating or disrupting SRP54, SGIl, and SGI2 genes.

The present disclosure is directed to compositions comprising mitochondrial optogenetics-based gene therapies and their methods of use. In some embodiments, a composition described herein comprises an expression vector comprising a first nucleic acid sequence encoding a channelrhodopsin fusion protein and a second nucleic acid sequence encoding a luciferase protein. In some cases, the first nucleic acid sequence and the second nucleic acid sequence are operably linked to an expression control sequence. In some instances, the channelrhodopsin fusion protein comprises a channelrhodopsin protein linked to an inner mitochondrial membrane-mitochondrial localization signal (IMM-MLS). In some implementations, when the expression vector is expressed, the luciferase protein is localized to the cytosol. In some cases, the IMM-MLS comprises a leading sequence from a mitochondrial inner membrane protein selected from ABCB10, ABCB140, Cytochrome C, and renal outer medullary potassium channel (ROMK).

Provided are sequence variants and fusion proteins of the photosensitive ion channel protein ChRmine and their use in treating retinal neurodegenerative diseases. These ChRmine variants and fusion proteins exhibit improved protein properties and enhanced photoresponsive characteristics compared with the wildtype ChRmine, therefore may provide effective optogenetic tools to improve the therapeutic treatment of the retinal neurodegenerative diseases.

Provided herein are stoichiometric Gas Vesicle Expression Systems (GVES), sets of polynucleotide constructs, and related vectors, cells, compositions, and methods configured for robust expression of Gas Vesicle Gene Clusters (GVGCs) in mammalian cells, particularly primary and immune cells. The GVES comprises distinct gene modules for the primary structural protein (gvpA / B) and assembly factors (AF1, AF2), operably configured to achieve a stoichiometric expression ratio, quantified by Dosage Index (DI), wherein the gvpA / B module is expressed at least 2-fold higher than the AF1 and AF2 modules.

The present invention provides recombinant AAV particles containing opsin transgenes for therapy of retinal diseases, as well as related compositions and methods.

The present invention addresses the problem of providing a modified channelrhodopsin having high ion permeability (photoreactivity). The problem is solved by substituting, of the three extracellular domains of a channelrhodopsin derived from volvox, the third extracellular domain from the N-terminal side, with a corresponding extracellular domain of channelrhodopsin-2 derived from Chlamydomonas reinhardtii.

The application provides a novel light-activated channel protein VR3.0 and uses thereof. The light-activated channel protein VR3.0 series provided in this application has a wide range of photosensitive wavelengths, higher sensitivity to light response, faster photoresponse kinetics, maintaining stable photocurrent signals under high-frequency stimulation while exhibiting good response amplitude and frequency under light stimulation of various wavelengths (especially white light, natural light). The light-activated channel protein VR3.0 series provided in this application has a clear therapeutic effect on retinal photoreceptor cell degenerative diseases, and can be used to prepare drugs for restoring the photoreceptor function of the retina, restoring the visual or photosensitive ability of subjects, and treating retinal degenerative diseases.

The invention provides an engineering dunaliella salina for producing astaxanthin based on system control logic reprogramming of an LSD transcription factor group, and belongs to the field of synthetic biology, the engineering dunaliella salina for producing astaxanthin is obtained by taking dunaliella salina as a starting algae strain and transferring the starting algae strain into carriers pGreen0029-Cmr-BKT-OE, pGreen0029-Cmr and DsLSD-OE-DsWRKY1-RNAi. The engineering dunaliella salina can efficiently synthesize the astaxanthin, and the biosynthesis efficiency of the astaxanthin is remarkably improved. The invention further provides a construction method and application of the astaxanthin-producing engineering dunaliella salina based on system control logic reprogramming of the LSD transcription factor group.

Provided herein are stoichiometric Gas Vesicle Expression Systems (GVES), sets of polynucleotide constructs, and related vectors, cells, compositions, and methods configured for robust expression of Gas Vesicle Gene Clusters (GVGCs) in mammalian cells, particularly primary and immune cells. The GVES comprises distinct gene modules for the primary structural protein (gvpA / B) and assembly factors (AF1, AF2), operably configured to achieve a stoichiometric expression ratio, quantified by Dosage Index (DI), wherein the gvpA / B module is expressed at least 2-fold higher than the AF1 and AF2 modules.

To provide a method for producing trans-polyisoprenoid (trans-1,4-polyisoprene) having a molecular weight of more than 105 in an enzymatically reactive manner.SOLUTION: The present invention provides a method for producing trans-polyisoprenoid, the method comprising a binding step for binding a trans-prenyltransferase (tPT) family protein capable of producing a product having a molecular weight of 104 or more under the condition where the protein is not bound to a lipid membrane to a lipid membrane in vitro.SELECTED DRAWING: None

This application provides a novel photosensitive channel protein VR2.0 and its use. [Solution] The VR2.0 series of photosensitive channel proteins provided in this application exhibits remarkable therapeutic effects in the treatment of retinal photoreceptor cell degeneration diseases. This novel photosensitive channel protein exhibits higher photosensitivity, faster photoresponse dynamics, and reduced side effects. Under the same photostimulation conditions, it can maintain a faster response frequency and a stable current signal even in high-frequency responses. This application offers a better option for the development of optogenetic therapies for retinal photoreceptor cell degeneration diseases and has significant application and promotional value.

The present invention provides recombinant AAV particles comprising an opsin transgene for therapy of retinal diseases, and related compositions and methods.

The present invention describes a method for processing fresh algae at ambient temperature by osmotic shocking the algae and treating the disrupted algae with an enzyme composition containing cell wall-degrading enzymes. This gentle process at ambient temperature allows for the isolation of algal proteins that have good solubility in the presence of salts and good foaming, emulsifying, and water-binding properties. Another advantage is that this protein isolation method enables cascade biorefineries, since after protein isolation, the remaining biomass can be treated with carbohydrate-degrading enzymes to produce high yields of clean biogas and a mineral-rich water stream for anaerobic digestion.

The invention discloses phycobiliprotein and / or red algae oligosaccharide extracted based on a biological soluble wall and a preparation method of the phycobiliprotein and / or the red algae oligosaccharide, and belongs to the technical field of biological functional component extraction. The invention provides a preparation method for simultaneously extracting phycobiliprotein and phycobiliprotein of red algae, which is high in efficiency, low in loss rate and suitable for process amplification, specifically, cell wall polysaccharide in the red algae is matched with a compound enzyme in a matching manner, and multiple enzymes are cooperated, so that the contradiction problem between high-temperature dissolution of the cell wall polysaccharide of the red algae and heat sensitivity of phycobiliprotein is effectively solved, and the yield of the phycobiliprotein and the oligosaccharide of the red algae is improved. The wall breaking efficiency is improved, and cell wall breaking is promoted to form porous and macroporous cell wall structures; the outward migration of intracellular substances such as phycobiliprotein is promoted, so that higher extraction efficiency is obtained. According to the preparation method provided by the invention, the extraction amount of phycobiliprotein reaches 224 g / kg after production conversion, and the dissociation loss of phycobiliprotein polymers is effectively reduced. In addition, the phycobiliprotein is efficiently extracted, meanwhile, the red algae oligosaccharide product is obtained, and the process economic benefit and the raw material utilization rate are improved. The preparation method provided by the invention does not need special equipment, is simple and convenient in process, green, environment-friendly, low in cost and beneficial to industrial production.

The invention relates to mutant light-inducible ion channels having improved properties as compared to the parent channel, nucleic acid constructs encoding same, expression vectors carrying the nucleic acid construct, cells comprising said nucleic acid construct or expression vector, and their respective uses, as well as non- human animals comprising the mutant light-inducible ion channel, the nucleic acid construct or the expression vector as disclosed herein.

The present disclosure describes systems and methods for extracting one or more compounds from aquatic plants, such as, seaweed, although other plants or plant types are possible. As described in more detail below, seaweed and certain other plants may contain useful compounds and materials, such as bromoform (or some other halogenated compound) and / or phycoerythrin (PE). The useful compounds may be extracted into a particular phase (e.g., an aqueous phase, an organic phase) and, in some cases, further purified. In some cases, a majority or an entirety of the seaweed can be used. That is, in some embodiments, useful compounds are extracted from the seaweed and the remaining seaweed itself (i.e., the seaweed remaining after the useful compounds have been extracted) may also be useful in that it may contain useful nutrients (e.g., protein) that can be used as a feedstock (e.g., an animal feedstock) and / or may be used as an additive to existing feedstock.

The present invention relates to a mutant ion channel capable of being activated by light (light-activated' ion channel) and having improved properties, nucleic acids and expression vectors encoding the mutant ion channel, cells comprising such nucleic acid or expression vector, devices containing the mutant ion channel, nucleic acid or expression vector as well as respective uses and methods.

This invention discloses an extraction device for phycoerythrin 545 extract based on ultrasonic disruption, relating to the field of phycoerythrin extraction technology. The device includes a soundproof box with an ultrasonic generator module at its top and an amplitude transformer at its bottom output end. A limiting groove is formed inside the soundproof box, and a limiting plate is slidably connected within the groove. A support plate is located at the end of the limiting plate, and a cooling cylinder is located above the support plate. A sample container is detachably connected to the middle of the cooling cylinder. A lifting assembly for driving the support plate to rise and fall is located on the bottom inside the soundproof box, and a rotating assembly for driving the cooling cylinder to rotate is located on the top of the support plate. This invention achieves uniform sample distribution in the sample container, improves the disruption effect, and effectively ensures the cooling effect of the sample solution during use. Furthermore, it improves the cleaning efficiency of the amplitude transformer after use.

The invention discloses a Griffithsin-IL18 fusion protein and a preparation method and application thereof.The Griffithsin-IL18 fusion protein comprises a Griffithsin protein fragment, an IL18 protein fragment and a flexible connecting peptide connecting the Griffithsin protein fragment and the IL18 protein fragment, the Griffithsin protein fragment is located at the N end of the fusion protein, and the IL18 protein fragment is located at the C end of the fusion protein. According to the present invention, the dual functions of virus infection blocking and immune activation are achieved, the Griffithsin fragment is specifically combined with host cell surface integrin to induce internalization and block virus endocytosis, and the IL-18 fragment activates NK cells so as to improve the overall antiviral efficiency and achieve the precise targeting intervention on the infected cells.

The application provides a novel light-sensitive channel protein VR2.0 and uses thereof. The light-sensitive channel protein VR2.0 series provided in this application demonstrates profound therapeutic effects in the treatment of retinal photoreceptor cell degenerative diseases. This novel light-sensitive channel protein exhibits enhanced photosensitivity, fast kinetics, and reduced side effects. Under identical photostimulation conditions, VR2.0 demonstrates faster response frequencies while maintaining stable photocurrent signals even during high-frequency stimulation. This application provides a better optogenetic therapeutic option for retinal photoreceptor cell degenerative diseases, and has significant application and promotion value.

The cascaded extraction of fractional components of an algal biomass includes an initial disruption of an algal cell mass and the centrifugation of the disrupted algal cell mass into each of an oil fraction, an aqueous fraction and a solid fraction. Thereafter, the oil fraction may be purified into an algal oil, while the aqueous fraction may be micro-filtrated and dried into a protein, and the solid fraction may be enzymatically digested, macerated, precipitated and dried into a chitosan.

The application provides a novel light-activated channel protein VR3.0 and uses thereof. The light-activated channel protein VR3.0 series provided in this application has a wide range of photosensitive wavelengths, higher sensitivity to light response, faster photoresponse kinetics, maintaining stable photocurrent signals under high-frequency stimulation while exhibiting good response amplitude and frequency under light stimulation of various wavelengths (especially white light, natural light). The light-activated channel protein VR3.0 series provided in this application has a clear therapeutic effect on retinal photoreceptor cell degenerative diseases, and can be used to prepare drugs for restoring the photoreceptor function of the retina, restoring the visual or photosensitive ability of subjects, and treating retinal degenerative diseases.

The object of the invention is a method for extracting, with high yield, phycobiliproteins from cyanobacterial and / or algal biomass, obtaining extracts in water or aqueous solutions characterised by high pigment concentration and a purity degree equal to or greater than the food / cosmetic grade (P≥2). The method is based on a process characterised by a step of breaking down the cyanobacterial / algal cells by ultrasonication in an aqueous solution of ammonium sulphate, and simultaneous extraction of water-soluble compounds other than phycobiliproteins, followed by an extraction step of the phycobiliproteins using water or aqueous solutions.

The invention discloses a phaeodactylum tricornutum high-temperature-resistant engineering algal strain and a construction method and application thereof, and is characterized in that the high-temperature-resistant engineering algal strain is phaeodactylum tricornutum of an overexpressed bd1311 gene, and the nucleotide sequence of the bd1311 gene is as shown in SEQ ID NO.1. The construction method of the algal strain comprises the following steps: by using pPha-T1 as a carrier, carrying out high-temperature-resistant engineering on the phaeodactylum tricornutum, and carrying out high-temperature-resistant engineering on the carrying out connection fusion on seamless cloning based on homologous recombination and the full length of the sequence of the bd1311 gene to obtain a bd1311 gene overexpression vector; designing a bd1311 gene overexpression amplification primer, carrying out amplification by taking cDNA of wild phaeodactylum tricornutum as a template to obtain a target fragment, and carrying out recombinant cloning on the target fragment and the enzyme-digested linearized vector; performing escherichia coli transformation on the recombinant vector, and then electrically transforming the recombinant vector into phaeodactylum tricornutum to obtain a phaeodactylum tricornutum high-temperature-resistant engineering algal strain; the fucoxanthin gene has the advantages that the fucoxanthin gene is resistant to high temperature in summer and is positively regulated and controlled.

Provided herein, inter alia, are variant lectin anti-viral polypeptides as well as compositions and methods for expressing and using the same for the treatment and prevention of viral disease in animals.