A production method for comprehensively utilizing oil-containing microalgae, comprising: using a high-pressure homogenizer to homogenize and break the wall of the microalgae at room temperature and high pressure. Transfer to the extraction tank, extract the microalgae oil under agitation, separate the liquid phase and the solid phase after centrifugation, wash the solid phase with water three times, add it to a spray dryer for drying to obtain microalgae powder. The liquid phase and the three times of washing liquid are added to the oil-water separator to separate into an oil phase and a water phase, and the oil phase is microalgae oil. The separated water phase is separated and purified to extract bioactive substances such as microalgae protein, microalgae polysaccharide, and microalgae liquid fertilizer. The invention has the advantages of low investment, simple operation, large-scale utilization of microalgae production method, and can produce microalgae oil, EPA, DHA, microalgae powder, microalgae protein, microalgae polysaccharide, microalgae liquid fertilizer and other high added value Semi-finished products.