245results about "Algae/lichens peptides" patented technology

The invention discloses a genetically engineered algae capable of resisting aquatic pathogenic bacteria as well as a construction method and application thereof, and is characterized in that the genetically engineered algae is phaeodactylum tricornutum into which cell wall energizing plasmids pPha-T1-J48054-eGFP-Linker-Q4 or pPha-T1-J48054-eGFP-Linker-Q6 are introduced, and the preparation method of the genetically engineered algae comprises the following steps: taking genome DNA of the phaeodactylum tricornutum as a template; the method comprises the following steps: constructing a directional expression plasmid pPha-T1-J48054-eGFP by amplifying a Phatr3J48054 coding sequence, inserting Q4 and Q6 coding sequences into the downstream of the eGFP of a directional expression vector, constructing a cell wall energizing plasmid, electrically transforming the cell wall energizing plasmid into phaeodactylum tricornutum, coating an f / 2 plate culture medium with transformed phaeodactylum tricornutum cells for culturing, screening positive algae strains, and screening the positive algae strains. The feed additive has the advantage of inhibiting or killing aquatic pathogenic bacteria.

Described in several exemplary embodiments herein are serum replacement compositions, cell culture media containing said serum replacement compositions, and uses thereof.

The invention discloses a heat shock transcription factor 49557 gene for increasing fucoxanthin content in phaeodactylum tricornutum and application thereof, and is characterized in that the nucleotide sequence of the 49557 gene is shown as SEQ ID NO: 3, the amino acid sequence of protein encoded by the 49557 gene is shown as SEQ ID NO: 4, the content of fucoxanthin synthesized by the 49557 gene is increased by knocking out the 49557 gene in the phaeodactylum tricornutum, and the content of fucoxanthin in the phaeodactylum tricornutum is increased. The method has the advantage that the fucoxanthin content in the phaeodactylum tricornutum can be remarkably increased.

The invention provides a chlamydomonas reinhardtii ABC transporter protein gene CrABCG as well as an encoding protein and application thereof. The chlamydomonas reinhardtii ABC transporter protein gene comprises CrABCG3 or CrABCG5, and the encoding protein of the chlamydomonas reinhardtii ABC transporter protein gene CrABCG is an ABC transporter protein The gene sequence is shown as SEQ ID NO: 1 or SEQ ID NO: 2. The invention finds that the CrABCG protein is related to carbon and nitrogen metabolism of chlamydomonas reinhardtii, preliminary research shows that the expression of the CrABCG3 gene of the chlamydomonas reinhardtii is improved after nitrogen limitation, the expression of the CrABCG5 gene is reduced and then improved, and the result shows that the CrABCG protein affects the transport, synthesis and accumulation of lipid substances. Therefore, an engineering algal strain with high lipid content can be obtained by screening and transforming the algal strain with high expression of the CrABCG gene.

The invention discloses a ferredoxin mutant and application of the ferredoxin mutant in synthesis of murine deoxycholic acid, and belongs to the technical field of biological catalytic enzymes. According to the invention, Escherichia coli is used as a chassis strain to express P450 enzyme OleP, a three-component redox partner library is established, and a redox partner PetH / PetF which is most adaptive to OleP is screened. A high-throughput method based on a characteristic light absorption value is developed, and the lithocholic acid and the murine deoxycholic acid can be rapidly and efficiently detected. And the ferredoxin mutant PetFF64D is screened through semi-rational design of the ferredoxin, so that the conversion rate of catalytically preparing the murine deoxycholic acid can be greatly improved. The method provides a new strategy for improving the biosynthesis of hydroxylated steroids.

The invention relates to the technical field of biomedicine, discloses mutant photo-induced ion channel ChR-2 protein, and further discloses a nucleic acid construct, an expression vector, a cell, related application and a computer model. The light-sensitive channel ChR2 protein mutant obtained by the invention has stronger light current, and the light-sensitive capability of the light-sensitive channel ChR2 protein mutant is at least improved by 100 times; besides, the invention also obtains a nucleotide sequence for coding the light-sensitive channel ChR2 protein mutant, constructs a recombinant expression vector, and obtains the light-sensitive channel ChR2 protein with higher expression quantity and stronger light sensitivity, and the light-sensitive channel ChR2 protein is very suitable for expression in cells of mammals (especially human); according to the invention, the rhodopsin in different channels is systematically studied by modifying the position G224 in the helix 6 of seven transmembrane helix motifs, which proves that the mutation of the position G224 in the helix 6 in WT ChR2 accelerates the photosensitivity of the channels, and the considered position is homologous in the helix 6 of the rhodopsin in different channels.

Aspects of the present disclosure relate to genetically modified plants and / or algae with increased carbon use efficiency as a result of an increased ability for bicarbonate to cross membranes within plant cells. Other aspects of the present disclosure relate to methods of making such plants and / or algae as well as cultivating these genetically modified plants to increase carbon use efficiency and / or growing these genetically modified algae to increase carbon use efficiency.

The invention discloses a heat shock transcription factor bd1650 gene for increasing the content of fucoxanthin in phaeodactylum tricornutum and application thereof, and is characterized in that the nucleotide sequence of the bd1650 gene is as shown in SEQ ID NO: 1, the amino acid sequence of protein encoded by the bd1650 gene is as shown in SEQ ID NO: 2, the content of the fucoxanthin synthesized by the bd1650 gene is increased by knocking out the bd1650 gene in the phaeodactylum tricornutum, and the content of the fucoxanthin in the phaeodactylum tricornutum is increased. The method has the advantage that the fucoxanthin content in the phaeodactylum tricornutum can be remarkably increased.

The invention discloses application of a CMIET protein and a coding gene thereof in improving manganese resistance of plants. The invention belongs to the technical field of biology, and particularly relates to application of CMIET protein and a coding gene thereof to improvement of manganese resistance of plants. The CMIET protein can regulate and control biological manganese resistance, and excessive manganese in Chlamydomonas chloroplast can be discharged to cytoplasm by regulating and controlling expression of the protein CMIET or regulating and controlling gene or regulating and controlling activity or content of the protein CMIET, so that balance of manganese ion concentration in the chloroplast is maintained, and the chloroplast is prevented from being affected by manganese toxicity; the normal photosynthesis capability of the cells is maintained, and the normal photosynthesis is ensured.

The invention discloses a flocculation-controllable genetic engineering alga and a construction method and application thereof, and is characterized in that the genetic engineering alga is phaeodactylum tricornutum into which an enabling plasmid pPha-J47869-EG01992-eGFP-FSP is introduced, the FSP is directionally expressed on the outer side of a phaeodactylum tricornutum cell, and the construction method comprises the following steps: constructing an induced expression plasmid pPha-J47869-eGFP and a directional expression plasmid pPha-J47869-EG01992-eGFP; artificially synthesizing a codon-optimized FSP coding sequence, inserting the codon-optimized FSP coding sequence into the downstream of the eGFP of the directional expression vector, and constructing an enabling plasmid; finally, the enabling plasmids are electrically transformed into phaeodactylum tricornutum, an f / 2 plate culture medium is coated with the phaeodactylum tricornutum for culture, positive algae strains are screened and identified, and the gene engineering algae with controllable flocculation are constructed and have the advantages that the gene engineering algae can be efficiently combined with white carbon black, and the enabling process of microalgae is efficiently coupled with the culture and collection process of the microalgae.

The invention provides a novel light-sensitive channel protein VR2.0 and application thereof. The photosensitive channel protein VR2.0 series provided by the invention has an obvious treatment effect in treatment of retina photoreceptor cell degenerative diseases. The novel light-sensitive channel protein has higher light sensitivity, fast dynamics and low side effect; under the same light stimulation condition, the response frequency is fast, and stable current signals can be kept under high frequency response. The application provides a better choice for developing photogenetic therapy for retina photoreceptor cell degenerative diseases, and has important application and popularization values.

The invention provides application of GRFT and fusion protein thereof in paramyxovirus or new coronavirus infection. The GRFT or the fusion protein GREK1 thereof can be used for preparing medicines for (a) preventing or treating paramyxovirus or new coronavirus infection, and / or (b) diseases caused by paramyxovirus or new coronavirus infection. The GRFT can comprise an amino acid sequence as shown in SEQ ID NO: 2, and the GREK1 can comprise an amino acid sequence as shown in SEQ ID NO: 1.

The invention discloses a flocculation-controllable genetic engineering alga and a construction method and application thereof, and is characterized in that the genetic engineering alga is phaeodactylum tricornutum into which an enabling plasmid pPha-J47869-EG01992-eGFP-FSP is introduced, the FSP is directionally expressed on the outer side of a phaeodactylum tricornutum cell, and the construction method comprises the following steps: constructing an induced expression plasmid pPha-J47869-eGFP and a directional expression plasmid pPha-J47869-EG01992-eGFP; artificially synthesizing a codon-optimized FSP coding sequence, inserting the codon-optimized FSP coding sequence into the downstream of the eGFP of the directional expression vector, and constructing an enabling plasmid; finally, the enabling plasmids are electrically transformed into phaeodactylum tricornutum, an f / 2 plate culture medium is coated with the phaeodactylum tricornutum for culture, positive algae strains are screened and identified, and the gene engineering algae with controllable flocculation are constructed and have the advantages that the gene engineering algae can be efficiently combined with white carbon black, and the enabling process of microalgae is efficiently coupled with the culture and collection process of the microalgae.

Provided herein are novel thermostable phycobiliproteins. These proteins may be stabilized by the introduction of disulfide bonds which stabilize the protein. Modified cells expressing these thermostable phycobiliproteins and methods of making them are also provided.

The present invention relates to: a Bacillus amyloliquefaciens Mu2 strain; and a chlorella protein extraction method using same. A chlorella protein extracted using the strain can be effectively used as an alternative protein material containing an abundance of constituent amino acids and free amino acids.

The present invention discloses a method for promoting the expression of selenoprotein genes in Chlamydomonas reinhardtii and increasing the yield of selenoproteins. S1. Inoculate Chlamydomonas reinhardtii algae with liquid culture medium at 20-30°C and 0-200 μmol / (m 2 S2, taking the Chlamydomonas reinhardtii culture solution, adding sodium selenite and abscisic acid, the abscisic acid concentration in the culture solution is 10-1000 μmol / L, at 20-30°C, 0-200 μmol / (m 2 ·s) Oscillating culture under light conditions. The present invention adds the plant hormone ABA during the selenium-enriched culture of Chlamydomonas reinhardtii, which not only does not affect the growth and biomass accumulation of microalgae, but also promotes the expression of selenoproteins. It is applied in the production of Chlamydomonas reinhardtii algae powder rich in selenoproteins, and can greatly increase the yield of selenoproteins.

The invention belongs to the technical field of haematococcus separation, and particularly relates to a method for sequentially separating polysaccharide and phycoerythrin from a haematococcus degreased algae residue water extract, and the method comprises the following steps: S1, pretreatment, S2, deep flocculation and primary separation of polysaccharide, and S3, specific precipitation of phycoerythrin. According to the method, a set of sequential separation process is constructed by adopting a self-made hydrophobic flocculant and a composite precipitation inducer, so that a high-purity polysaccharide product and a high-activity phycoerythrin concentrated solution can be synchronously obtained from a haematococcus degreased algae residue water extract. According to the method, deep flocculation of polysaccharide, specific precipitation of phycoerythrin and respective subsequent refining steps are integrated into an ordered whole process, so that the product in the previous step can be directly treated in the next step in an optimal state, and the loss possibly caused by storage and transfer of intermediate materials is reduced; through condition matching and cooperation among the steps, the purity of the final product is improved.

The invention relates to the technical field of plant genetic engineering, in particular to application of an algae NySAT2 gene in regulation and control of alfalfa crude protein and detergent fiber. According to the invention, a sequence after codon optimization of an NySAT2 gene coding region is obtained by using gene synthesis and codon optimization technologies, an algae NySAT2 gene overexpression vector is constructed, a target gene is introduced into an alfalfa genome by using an agrobacterium rhizogenes mediated genetic transformation method, and a transgenic plant is obtained by using a genetic transformation system. Molecular identification and phenotypic analysis show that on the premise of keeping normal growth vigor (plant height and biomass have no significant difference from those of a wild type), the crude protein content of a medicago sativa plant with the overexpressed NySAT2 gene is remarkably increased compared with that of the wild type medicago sativa plant, and meanwhile, the contents of acid detergent fibers and neutral detergent fibers are remarkably reduced, so that the content of the crude protein in the medicago sativa plant with the overexpressed NySAT2 gene is remarkably increased. The forage quality and the feeding value of the medicago sativa are effectively improved.

The invention discloses construction and activity detection methods of semisynthetic chassis cells for genetic material expansion based on vibrio natrieti. The VnDX / pET23b-PtNTT2 (66-575) cell has a relatively wide nucleoside triphosphate transport active substrate spectrum, and has recognition and transport activities on natural nucleoside triphosphate (dNTPs and NTPs), pentose modified nucleoside triphosphate (2 '-OMe-NTPs and 2'-F-NTPs), and nucleoside triphosphate (dTPT3TP, dNaMTP, dCNMOTP, TPT3TP and NaMTP) containing non-natural basic groups, and the VnDX / pET23b-PtNTT2 (66-575) cell has the recognition and transport activities on the natural nucleoside triphosphate (dNTPs and NTPs) and the pentose modified nucleoside triphosphate (2 '-OMe-NTPs and 2'-F-NTPs). By means of the transport characteristic of the nucleoside triphosphate transporter PtNTT2 (66-575) and by detecting the content of ATP transported out of cells within the same time, the relative strength of the transport activity of various nucleoside triphosphate of the PtNTT2 (66-575) is simply, conveniently and rapidly determined.

The present invention provides a fusion protein BVP10‑GS‑R5 and a nano-silicon dioxide acaricide and a preparation method and application thereof, belonging to the technical field of biological pesticides. The amino acid sequence of the fusion protein BVP10‑GS‑R5 of the present invention is shown in SEQ ID NO.2. The present invention utilizes R5 peptide and acaricide protein BVP10 to construct a fusion protein, and then uses the fusion protein to prepare a nano-silicon dioxide acaricide, which can react at room temperature and pressure, with mild and controllable conditions, good biocompatibility and environmental benefits, and solves the problem of harsh preparation conditions of nano-silicon dioxide preparations in the prior art. And compared with the fusion protein BVP10‑GS‑R5, the nano-silicon dioxide acaricide prepared by the present invention has better sustained release, adhesion and acaricide activity, and is suitable for large-scale promotion and application.

Certain aspects of the present disclosure relate to industrial scale production and recovery of lectin proteins. Certain aspects relate to recombinant gram-positive bacterial cells that produce heterologous lectin proteins. Certain other aspects relate to compositions and methods for recovering and / or purifying one or more lectins and lectin formulations with improved purity thereof.

This invention provides the nucleic acid molecules encoding novel small monomeric near-infrared fluorescent protein miRFP670nano, variants and derivatives thereof as well as proteins and peptides encoded by these nucleic acids. The invention also relates to derivatives, homologues, or mutants of the specific proteins referenced above as well as fragments of the nucleic acids and the peptides encoded thereby. The invention further relates to host-cells, stable cell lines and transgenic organisms comprising above-referenced nucleic acid molecules. The present invention also refers to methods of making and using small monomeric near-infrared fluorescent proteins derived from cyanobacteriochromes. The presented protein and its derivatives find use in a variety of applications and approaches, including labeling of biomolecules, cells or cell organelles, detecting protein-protein interactions, and generation of genetically encoded fluorescent biosensor.

The present invention provides novel DNA molecules and constructs, including nucleotide sequences thereof, for modulating gene expression in plants and plant cells. The invention also provides transgenic plants, plant cells, plant parts, seeds, and commodity products comprising DNA molecules operably linked to heterologous transcribable polynucleotides, as well as methods of their use.

The present invention discloses an antimicrobial peptide PmAMP12 from pearl columnar algae, and its amino acid sequence is shown in SEQ ID NO: 2. The present invention also discloses its application in antibacterial. Expressing PmAMP12 on the cell surface of Agrobacterium tumefaciens can destroy the integrity of Agrobacterium tumefaciens cells; PmAMP12 exhibits inhibitory activity against a variety of Gram-negative bacteria, Gram-positive bacteria and fungi, and studies have also found that PmAMP12 can directly destroy the integrity of cells and cause changes inside cells. The antimicrobial peptide PmAMP12 of the present invention provides a new and excellent biological resource for the development of green biological pesticides, and has great application potential in agricultural production.

The invention provides recombinant algal mutants that have a genetic modification to a gene or nucleic acid sequence encoding a WD40 repeat containing protein or domain. The genetic modification of one or more nucleic acid sequences encoding a WD40 repeat containing protein or domain results in a mutant organism with increased lipid productivity and / or higher biomass productivity (as measured by total organic carbon). The genetic modification can be a gene attenuation or functional deletion. The lipid products of these mutants can be utilized as biofuels or for other specialty chemical products. Methods of making and using the recombinant algal mutants and methods of producing lipids are also disclosed.

The application of Aida gene in the tolerance of Chlamydomonas reinhardtii to copper ion stress, the nucleotide sequence of Aida gene is shown as SEQ ID No 1, the nucleotide sequence of its coding region is shown as SEQ ID No 2; the amino acid sequence of Aida protein encoded by Aida gene is shown as SEQ ID No 3. The Chlamydomonas reinhardtii cell strain obtained after the mutation of Aida gene in the present invention, through the determination of copper ion growth curve and adsorption efficiency, found that the mutant algae strain has faster growth rate, higher adsorption efficiency, and higher copper ion tolerance. The present invention provides convenience for the subsequent in-depth study of how Aida gene affects various oxidative stress reactions and metal transport, the expression and activity of metabolism-related proteins and related stress organelle functions in Chlamydomonas reinhardtii, and will later lay a solid foundation for the theoretical study of the heavy metal copper metabolism mechanism of Chlamydomonas reinhardtii and the application research of heavy metal bioremediation.

Methods and kits are provided for producing Griffithsin. The methods include providing a genetically modified microorganism comprising a gene encoding Griffithsin protein operably linked to an inducible promotor and growing the genetically modified microorganism under conditions that induce the promotor and cause expression of griffithisin. The Griffithsin is purified by releasing Griffithsin from the microorganism by cellular disruption, performing a precipitation step to remove contaminating protein and nucleic acids, and performing an anion exchange chromatography step.

The invention discloses a culture, photostimulation and signal processing method of a three-dimensional on-chip brain. The three-dimensional on-chip brain is transfected by using adeno-associated viruses which can specifically express light-sensitive channel proteins, so that cells of the three-dimensional on-chip brain can express the light-sensitive channel proteins on cell membranes of the cells, and 470nm blue light emitted by a light source irradiates the three-dimensional on-chip brain expressing the light-sensitive channel proteins; according to the method, light-sensitive channel protein on a cell membrane is opened, ions in a culture medium enter cells, the ion concentration in the environment is transiently changed, the change of the ions can be detected through a multi-electrode array, and the change range is analyzed; the physiological characteristics of the three-dimensional on-chip brain can be obtained, the function network structure of the three-dimensional on-chip brain is analyzed, and the biological intelligent function of the three-dimensional on-chip brain is disclosed.