127results about "Algae/lichens peptides" patented technology

The invention relates to the technical field of biomedicine, discloses mutant photo-induced ion channel ChR-2 protein, and further discloses a nucleic acid construct, an expression vector, a cell, related application and a computer model. The light-sensitive channel ChR2 protein mutant obtained by the invention has stronger light current, and the light-sensitive capability of the light-sensitive channel ChR2 protein mutant is at least improved by 100 times; besides, the invention also obtains a nucleotide sequence for coding the light-sensitive channel ChR2 protein mutant, constructs a recombinant expression vector, and obtains the light-sensitive channel ChR2 protein with higher expression quantity and stronger light sensitivity, and the light-sensitive channel ChR2 protein is very suitable for expression in cells of mammals (especially human); according to the invention, the rhodopsin in different channels is systematically studied by modifying the position G224 in the helix 6 of seven transmembrane helix motifs, which proves that the mutation of the position G224 in the helix 6 in WT ChR2 accelerates the photosensitivity of the channels, and the considered position is homologous in the helix 6 of the rhodopsin in different channels.

The invention belongs to the technical field of haematococcus separation, and particularly relates to a method for sequentially separating polysaccharide and phycoerythrin from a haematococcus degreased algae residue water extract, and the method comprises the following steps: S1, pretreatment, S2, deep flocculation and primary separation of polysaccharide, and S3, specific precipitation of phycoerythrin. According to the method, a set of sequential separation process is constructed by adopting a self-made hydrophobic flocculant and a composite precipitation inducer, so that a high-purity polysaccharide product and a high-activity phycoerythrin concentrated solution can be synchronously obtained from a haematococcus degreased algae residue water extract. According to the method, deep flocculation of polysaccharide, specific precipitation of phycoerythrin and respective subsequent refining steps are integrated into an ordered whole process, so that the product in the previous step can be directly treated in the next step in an optimal state, and the loss possibly caused by storage and transfer of intermediate materials is reduced; through condition matching and cooperation among the steps, the purity of the final product is improved.

The invention relates to the technical field of plant genetic engineering, in particular to application of an algae NySAT2 gene in regulation and control of alfalfa crude protein and detergent fiber. According to the invention, a sequence after codon optimization of an NySAT2 gene coding region is obtained by using gene synthesis and codon optimization technologies, an algae NySAT2 gene overexpression vector is constructed, a target gene is introduced into an alfalfa genome by using an agrobacterium rhizogenes mediated genetic transformation method, and a transgenic plant is obtained by using a genetic transformation system. Molecular identification and phenotypic analysis show that on the premise of keeping normal growth vigor (plant height and biomass have no significant difference from those of a wild type), the crude protein content of a medicago sativa plant with the overexpressed NySAT2 gene is remarkably increased compared with that of the wild type medicago sativa plant, and meanwhile, the contents of acid detergent fibers and neutral detergent fibers are remarkably reduced, so that the content of the crude protein in the medicago sativa plant with the overexpressed NySAT2 gene is remarkably increased. The forage quality and the feeding value of the medicago sativa are effectively improved.

The invention provides recombinant algal mutants that have a genetic modification to a gene or nucleic acid sequence encoding a WD40 repeat containing protein or domain. The genetic modification of one or more nucleic acid sequences encoding a WD40 repeat containing protein or domain results in a mutant organism with increased lipid productivity and / or higher biomass productivity (as measured by total organic carbon). The genetic modification can be a gene attenuation or functional deletion. The lipid products of these mutants can be utilized as biofuels or for other specialty chemical products. Methods of making and using the recombinant algal mutants and methods of producing lipids are also disclosed.

Methods and kits are provided for producing Griffithsin. The methods include providing a genetically modified microorganism comprising a gene encoding Griffithsin protein operably linked to an inducible promotor and growing the genetically modified microorganism under conditions that induce the promotor and cause expression of griffithisin. The Griffithsin is purified by releasing Griffithsin from the microorganism by cellular disruption, performing a precipitation step to remove contaminating protein and nucleic acids, and performing an anion exchange chromatography step.

Disclosed herein is a method for enhancing immune response of a vaccine. The method includes preparing a Phycocyanin compound. The Phycocyanin compound includes at least one of C-phycocyanin (C-PC), Phycoerythrin (PE), Allophycocyanin (APC), phycocyanobilin (PCB), and combinations thereof. The method further includes administering the prepared Phycocyanin along with a vaccine composition to a living body.

Mutant photosynthetic organisms having reduced chlorophyll and increased photosynthetic efficiency are provided. The mutant strains have mutated or attenuated: chloroplastic SRP54 gene and SGIl gene; chloroplastic SRP54 gene and SGI2 gene; chloroplastic SRP54 gene, SGIl, and SGI2 genes are disclosed. The mutant photosynthetic organisms exhibit increased productivity with respect to wild-type strains. Also provided are mutant photosynthetic organisms having mutated or attenuated cytosolic SRP54 genes. Provided herein are methods of producing biomass and other products such as lipids using strains having mutations in an SRP54 gene, SGIl, SGI2 genes, a combination of SGI1 / SRP54, and a combination of SGI2 and SRP54 genes. Also included are constructs and methods for attenuating or disrupting SRP54, SGIl, and SGI2 genes.

The present disclosure is directed to compositions comprising mitochondrial optogenetics-based gene therapies and their methods of use. In some embodiments, a composition described herein comprises an expression vector comprising a first nucleic acid sequence encoding a channelrhodopsin fusion protein and a second nucleic acid sequence encoding a luciferase protein. In some cases, the first nucleic acid sequence and the second nucleic acid sequence are operably linked to an expression control sequence. In some instances, the channelrhodopsin fusion protein comprises a channelrhodopsin protein linked to an inner mitochondrial membrane-mitochondrial localization signal (IMM-MLS). In some implementations, when the expression vector is expressed, the luciferase protein is localized to the cytosol. In some cases, the IMM-MLS comprises a leading sequence from a mitochondrial inner membrane protein selected from ABCB10, ABCB140, Cytochrome C, and renal outer medullary potassium channel (ROMK).

The invention discloses an application of a transcription factor ChZF7 of a basophilic microalgae Chlorella sp. BLD in regulation and control of salt tolerance of microalgae. According to the invention, the coding gene of the transcription factor ChZF7 is transferred into chlamydomonas reinhardtii by using a transgenic technology after intron insertion and codon optimization, so that transgenic chlamydomonas reinhardtii is obtained. The salt tolerance of the chlamydomonas reinhardtii with the over-expressed ChZF7 gene is remarkably improved, the accumulation of neutral fat and active oxygen under the stress of 225mM salt is less than that of the chlamydomonas reinhardtii in a control group, the content of penetrating substance proline is increased, in addition, the change of cell components in the chlamydomonas reinhardtii under the stress of salt can be weakened by the over-expressed ChZF7 gene, and the salt tolerance of the chlamydomonas reinhardtii is improved. The transcription factor ChZF7 and the gene coded by the transcription factor ChZF7 can regulate and control the salt tolerance of the chlamydomonas reinhardtii, a foundation is laid for genetic improvement and directed molecular breeding of the microalgae, the transcription factor ChZF7 plays an important role in improvement of the salt tolerance of the microalgae, reutilization of salinized water resources is facilitated, and the transcription factor ChZF7 and the gene coded by the transcription factor ChZF7 have wide application prospects.

Provided is a polypeptide combination, which comprises a Rubisco polypeptide from a species of the family Chlamydomonadaceae, and a fusion polypeptide comprising an EPYC1 polypeptide of a species of the family Chlamydomonadaceae and a mineralizing peptide, wherein the mineralizing peptide can convert a metal-containing compound into a compound capable of converting light energy into chemical energy, such as a metal oxide. Further provided are a polynucleotide or a vector encoding the polypeptide combination, a microorganism containing the polypeptide combination, the polynucleotide or the vector, and a method of using the microorganism.

Provided are sequence variants and fusion proteins of the photosensitive ion channel protein ChRmine and their use in treating retinal neurodegenerative diseases. These ChRmine variants and fusion proteins exhibit improved protein properties and enhanced photoresponsive characteristics compared with the wildtype ChRmine, therefore may provide effective optogenetic tools to improve the therapeutic treatment of the retinal neurodegenerative diseases.

Provided herein are stoichiometric Gas Vesicle Expression Systems (GVES), sets of polynucleotide constructs, and related vectors, cells, compositions, and methods configured for robust expression of Gas Vesicle Gene Clusters (GVGCs) in mammalian cells, particularly primary and immune cells. The GVES comprises distinct gene modules for the primary structural protein (gvpA / B) and assembly factors (AF1, AF2), operably configured to achieve a stoichiometric expression ratio, quantified by Dosage Index (DI), wherein the gvpA / B module is expressed at least 2-fold higher than the AF1 and AF2 modules.

The present invention addresses the issue of providing a modified photoreceptive chloride channel that has a narrow wavelength sensitivity range, has both short τon and short τoff, and has excellent photoreactivity. The solution is a polypeptide in which a region from the fourth transmembrane domain to the sixth transmembrane domain, counted from an N terminal-side of a Guillardia theta-derived photoreceptive chloride channel-1 (GtACR1), is replaced by a region corresponding to a Guillardia theta-derived photoreceptive chloride channel-2 (GtACR2).

The present invention relates to a forebrain assembloid that exhibits maturity similar to that of the human brain and expresses ChR2 to enable optogenetic analysis and, more specifically, to: a method for producing a forebrain assembloid that enables optogenetic analysis; a forebrain assembloid produced by the method; and a forebrain assembloid having a single rosette structure, comprising six cortical layers and cavities, wherein the cortical layers include glial cells and uniformly express ChR2.

The invention relates to a process for preparing a cyanobacterium extract, preferably spirulina, comprising phycocyanin enriched in trace elements. The invention also relates to an extract obtained by the process according to the invention, a composition comprising said extract and its use. Figure 1

The present disclosure provides light-responsive polypeptides, and nucleic acids comprising nucleotide sequences encoding the light-responsive polypeptides. The present disclosure provides methods, devices, and systems for controlling the activity of a cell expressing a light-responsive polypeptide of the present disclosure.

The present invention relates to a composition for leaching lithium, nickel, or cobalt using a Chlorella vulgaris strain, and to a method for leaching lithium, nickel, or cobalt.

The present invention provides recombinant AAV particles containing opsin transgenes for therapy of retinal diseases, as well as related compositions and methods.

The present invention addresses the problem of providing a modified channelrhodopsin having high ion permeability (photoreactivity). The problem is solved by substituting, of the three extracellular domains of a channelrhodopsin derived from volvox, the third extracellular domain from the N-terminal side, with a corresponding extracellular domain of channelrhodopsin-2 derived from Chlamydomonas reinhardtii.

Disclosed are immunogens for stimulating an immune response to coronaviruses. The immunogens can be non-receptor binding domain (RED) epitopes. The immunogens can be grafted into a scaffold polypeptide to produce an epitope scaffold. Antibodies induced by the immunogens are disclosed.

The application provides a novel light-activated channel protein VR3.0 and uses thereof. The light-activated channel protein VR3.0 series provided in this application has a wide range of photosensitive wavelengths, higher sensitivity to light response, faster photoresponse kinetics, maintaining stable photocurrent signals under high-frequency stimulation while exhibiting good response amplitude and frequency under light stimulation of various wavelengths (especially white light, natural light). The light-activated channel protein VR3.0 series provided in this application has a clear therapeutic effect on retinal photoreceptor cell degenerative diseases, and can be used to prepare drugs for restoring the photoreceptor function of the retina, restoring the visual or photosensitive ability of subjects, and treating retinal degenerative diseases.

The invention provides an engineering dunaliella salina for producing astaxanthin based on system control logic reprogramming of an LSD transcription factor group, and belongs to the field of synthetic biology, the engineering dunaliella salina for producing astaxanthin is obtained by taking dunaliella salina as a starting algae strain and transferring the starting algae strain into carriers pGreen0029-Cmr-BKT-OE, pGreen0029-Cmr and DsLSD-OE-DsWRKY1-RNAi. The engineering dunaliella salina can efficiently synthesize the astaxanthin, and the biosynthesis efficiency of the astaxanthin is remarkably improved. The invention further provides a construction method and application of the astaxanthin-producing engineering dunaliella salina based on system control logic reprogramming of the LSD transcription factor group.

Provided herein are stoichiometric Gas Vesicle Expression Systems (GVES), sets of polynucleotide constructs, and related vectors, cells, compositions, and methods configured for robust expression of Gas Vesicle Gene Clusters (GVGCs) in mammalian cells, particularly primary and immune cells. The GVES comprises distinct gene modules for the primary structural protein (gvpA / B) and assembly factors (AF1, AF2), operably configured to achieve a stoichiometric expression ratio, quantified by Dosage Index (DI), wherein the gvpA / B module is expressed at least 2-fold higher than the AF1 and AF2 modules.

The present invention relates to a composition for leaching lithium, nickel or cobalt, and a method for leaching lithium, nickel or cobalt, using a Chlorella vulgaris strain.

To provide a method for producing trans-polyisoprenoid (trans-1,4-polyisoprene) having a molecular weight of more than 105 in an enzymatically reactive manner.SOLUTION: The present invention provides a method for producing trans-polyisoprenoid, the method comprising a binding step for binding a trans-prenyltransferase (tPT) family protein capable of producing a product having a molecular weight of 104 or more under the condition where the protein is not bound to a lipid membrane to a lipid membrane in vitro.SELECTED DRAWING: None

Disclosed is a chlorophyll-deficient strain of Chlorella microalgae having a protein content of at least 50% w / w. Also disclosed is a method of producing a chlorophyll-deficient strain of Chlorella microalgae having a protein content of at least 50% w / w. Also disclosed is a composition comprising an algae biomass derived from the aforementioned chlorophyll-deficient strain of Chlorella microalgae or obtained by performing aforementioned method.

This application provides a novel photosensitive channel protein VR2.0 and its use. [Solution] The VR2.0 series of photosensitive channel proteins provided in this application exhibits remarkable therapeutic effects in the treatment of retinal photoreceptor cell degeneration diseases. This novel photosensitive channel protein exhibits higher photosensitivity, faster photoresponse dynamics, and reduced side effects. Under the same photostimulation conditions, it can maintain a faster response frequency and a stable current signal even in high-frequency responses. This application offers a better option for the development of optogenetic therapies for retinal photoreceptor cell degeneration diseases and has significant application and promotional value.

The present invention provides recombinant AAV particles comprising an opsin transgene for therapy of retinal diseases, and related compositions and methods.

The present invention describes a method for processing fresh algae at ambient temperature by osmotic shocking the algae and treating the disrupted algae with an enzyme composition containing cell wall-degrading enzymes. This gentle process at ambient temperature allows for the isolation of algal proteins that have good solubility in the presence of salts and good foaming, emulsifying, and water-binding properties. Another advantage is that this protein isolation method enables cascade biorefineries, since after protein isolation, the remaining biomass can be treated with carbohydrate-degrading enzymes to produce high yields of clean biogas and a mineral-rich water stream for anaerobic digestion.