184results about "Algae/lichens peptides" patented technology

The invention relates to the technical field of biomedicine, discloses mutant photo-induced ion channel ChR-2 protein, and further discloses a nucleic acid construct, an expression vector, a cell, related application and a computer model. The light-sensitive channel ChR2 protein mutant obtained by the invention has stronger light current, and the light-sensitive capability of the light-sensitive channel ChR2 protein mutant is at least improved by 100 times; besides, the invention also obtains a nucleotide sequence for coding the light-sensitive channel ChR2 protein mutant, constructs a recombinant expression vector, and obtains the light-sensitive channel ChR2 protein with higher expression quantity and stronger light sensitivity, and the light-sensitive channel ChR2 protein is very suitable for expression in cells of mammals (especially human); according to the invention, the rhodopsin in different channels is systematically studied by modifying the position G224 in the helix 6 of seven transmembrane helix motifs, which proves that the mutation of the position G224 in the helix 6 in WT ChR2 accelerates the photosensitivity of the channels, and the considered position is homologous in the helix 6 of the rhodopsin in different channels.

Aspects of the present disclosure relate to genetically modified plants and / or algae with increased carbon use efficiency as a result of an increased ability for bicarbonate to cross membranes within plant cells. Other aspects of the present disclosure relate to methods of making such plants and / or algae as well as cultivating these genetically modified plants to increase carbon use efficiency and / or growing these genetically modified algae to increase carbon use efficiency.

The invention discloses application of a CMIET protein and a coding gene thereof in improving manganese resistance of plants. The invention belongs to the technical field of biology, and particularly relates to application of CMIET protein and a coding gene thereof to improvement of manganese resistance of plants. The CMIET protein can regulate and control biological manganese resistance, and excessive manganese in Chlamydomonas chloroplast can be discharged to cytoplasm by regulating and controlling expression of the protein CMIET or regulating and controlling gene or regulating and controlling activity or content of the protein CMIET, so that balance of manganese ion concentration in the chloroplast is maintained, and the chloroplast is prevented from being affected by manganese toxicity; the normal photosynthesis capability of the cells is maintained, and the normal photosynthesis is ensured.

The invention discloses a flocculation-controllable genetic engineering alga and a construction method and application thereof, and is characterized in that the genetic engineering alga is phaeodactylum tricornutum into which an enabling plasmid pPha-J47869-EG01992-eGFP-FSP is introduced, the FSP is directionally expressed on the outer side of a phaeodactylum tricornutum cell, and the construction method comprises the following steps: constructing an induced expression plasmid pPha-J47869-eGFP and a directional expression plasmid pPha-J47869-EG01992-eGFP; artificially synthesizing a codon-optimized FSP coding sequence, inserting the codon-optimized FSP coding sequence into the downstream of the eGFP of the directional expression vector, and constructing an enabling plasmid; finally, the enabling plasmids are electrically transformed into phaeodactylum tricornutum, an f / 2 plate culture medium is coated with the phaeodactylum tricornutum for culture, positive algae strains are screened and identified, and the gene engineering algae with controllable flocculation are constructed and have the advantages that the gene engineering algae can be efficiently combined with white carbon black, and the enabling process of microalgae is efficiently coupled with the culture and collection process of the microalgae.

The invention provides a novel light-sensitive channel protein VR2.0 and application thereof. The photosensitive channel protein VR2.0 series provided by the invention has an obvious treatment effect in treatment of retina photoreceptor cell degenerative diseases. The novel light-sensitive channel protein has higher light sensitivity, fast dynamics and low side effect; under the same light stimulation condition, the response frequency is fast, and stable current signals can be kept under high frequency response. The application provides a better choice for developing photogenetic therapy for retina photoreceptor cell degenerative diseases, and has important application and popularization values.

The invention provides application of GRFT and fusion protein thereof in paramyxovirus or new coronavirus infection. The GRFT or the fusion protein GREK1 thereof can be used for preparing medicines for (a) preventing or treating paramyxovirus or new coronavirus infection, and / or (b) diseases caused by paramyxovirus or new coronavirus infection. The GRFT can comprise an amino acid sequence as shown in SEQ ID NO: 2, and the GREK1 can comprise an amino acid sequence as shown in SEQ ID NO: 1.

The present invention relates to: a Bacillus amyloliquefaciens Mu2 strain; and a chlorella protein extraction method using same. A chlorella protein extracted using the strain can be effectively used as an alternative protein material containing an abundance of constituent amino acids and free amino acids.

The invention belongs to the technical field of haematococcus separation, and particularly relates to a method for sequentially separating polysaccharide and phycoerythrin from a haematococcus degreased algae residue water extract, and the method comprises the following steps: S1, pretreatment, S2, deep flocculation and primary separation of polysaccharide, and S3, specific precipitation of phycoerythrin. According to the method, a set of sequential separation process is constructed by adopting a self-made hydrophobic flocculant and a composite precipitation inducer, so that a high-purity polysaccharide product and a high-activity phycoerythrin concentrated solution can be synchronously obtained from a haematococcus degreased algae residue water extract. According to the method, deep flocculation of polysaccharide, specific precipitation of phycoerythrin and respective subsequent refining steps are integrated into an ordered whole process, so that the product in the previous step can be directly treated in the next step in an optimal state, and the loss possibly caused by storage and transfer of intermediate materials is reduced; through condition matching and cooperation among the steps, the purity of the final product is improved.

The invention relates to the technical field of plant genetic engineering, in particular to application of an algae NySAT2 gene in regulation and control of alfalfa crude protein and detergent fiber. According to the invention, a sequence after codon optimization of an NySAT2 gene coding region is obtained by using gene synthesis and codon optimization technologies, an algae NySAT2 gene overexpression vector is constructed, a target gene is introduced into an alfalfa genome by using an agrobacterium rhizogenes mediated genetic transformation method, and a transgenic plant is obtained by using a genetic transformation system. Molecular identification and phenotypic analysis show that on the premise of keeping normal growth vigor (plant height and biomass have no significant difference from those of a wild type), the crude protein content of a medicago sativa plant with the overexpressed NySAT2 gene is remarkably increased compared with that of the wild type medicago sativa plant, and meanwhile, the contents of acid detergent fibers and neutral detergent fibers are remarkably reduced, so that the content of the crude protein in the medicago sativa plant with the overexpressed NySAT2 gene is remarkably increased. The forage quality and the feeding value of the medicago sativa are effectively improved.

The present invention provides a fusion protein BVP10‑GS‑R5 and a nano-silicon dioxide acaricide and a preparation method and application thereof, belonging to the technical field of biological pesticides. The amino acid sequence of the fusion protein BVP10‑GS‑R5 of the present invention is shown in SEQ ID NO.2. The present invention utilizes R5 peptide and acaricide protein BVP10 to construct a fusion protein, and then uses the fusion protein to prepare a nano-silicon dioxide acaricide, which can react at room temperature and pressure, with mild and controllable conditions, good biocompatibility and environmental benefits, and solves the problem of harsh preparation conditions of nano-silicon dioxide preparations in the prior art. And compared with the fusion protein BVP10‑GS‑R5, the nano-silicon dioxide acaricide prepared by the present invention has better sustained release, adhesion and acaricide activity, and is suitable for large-scale promotion and application.

The invention provides recombinant algal mutants that have a genetic modification to a gene or nucleic acid sequence encoding a WD40 repeat containing protein or domain. The genetic modification of one or more nucleic acid sequences encoding a WD40 repeat containing protein or domain results in a mutant organism with increased lipid productivity and / or higher biomass productivity (as measured by total organic carbon). The genetic modification can be a gene attenuation or functional deletion. The lipid products of these mutants can be utilized as biofuels or for other specialty chemical products. Methods of making and using the recombinant algal mutants and methods of producing lipids are also disclosed.

Methods and kits are provided for producing Griffithsin. The methods include providing a genetically modified microorganism comprising a gene encoding Griffithsin protein operably linked to an inducible promotor and growing the genetically modified microorganism under conditions that induce the promotor and cause expression of griffithisin. The Griffithsin is purified by releasing Griffithsin from the microorganism by cellular disruption, performing a precipitation step to remove contaminating protein and nucleic acids, and performing an anion exchange chromatography step.

Disclosed herein is a method for enhancing immune response of a vaccine. The method includes preparing a Phycocyanin compound. The Phycocyanin compound includes at least one of C-phycocyanin (C-PC), Phycoerythrin (PE), Allophycocyanin (APC), phycocyanobilin (PCB), and combinations thereof. The method further includes administering the prepared Phycocyanin along with a vaccine composition to a living body.

Provided are novel methods for purifying phycobiliproteins, particularly acid pH-resistant phycobiliproteins, the resulting phycobiliproteins, and uses thereof. [Solution] A method for purifying acid pH-resistant phycobiliproteins from a crude extract of acid pH-resistant phycobiliproteins from cells of phycobiliprotein-producing microorganisms, characterized in that the method comprises the steps of: a) adjusting the pH of the crude extract of acid pH-resistant phycobiliproteins to a pH of less than 6 to precipitate organic matter other than the acid pH-resistant phycobiliproteins; b) recovering a supernatant containing the acid pH-resistant phycobiliproteins; and c) separating the acid pH-resistant phycobiliproteins from the supernatant.

Mutant photosynthetic organisms having reduced chlorophyll and increased photosynthetic efficiency are provided. The mutant strains have mutated or attenuated: chloroplastic SRP54 gene and SGIl gene; chloroplastic SRP54 gene and SGI2 gene; chloroplastic SRP54 gene, SGIl, and SGI2 genes are disclosed. The mutant photosynthetic organisms exhibit increased productivity with respect to wild-type strains. Also provided are mutant photosynthetic organisms having mutated or attenuated cytosolic SRP54 genes. Provided herein are methods of producing biomass and other products such as lipids using strains having mutations in an SRP54 gene, SGIl, SGI2 genes, a combination of SGI1 / SRP54, and a combination of SGI2 and SRP54 genes. Also included are constructs and methods for attenuating or disrupting SRP54, SGIl, and SGI2 genes.

The present disclosure is directed to compositions comprising mitochondrial optogenetics-based gene therapies and their methods of use. In some embodiments, a composition described herein comprises an expression vector comprising a first nucleic acid sequence encoding a channelrhodopsin fusion protein and a second nucleic acid sequence encoding a luciferase protein. In some cases, the first nucleic acid sequence and the second nucleic acid sequence are operably linked to an expression control sequence. In some instances, the channelrhodopsin fusion protein comprises a channelrhodopsin protein linked to an inner mitochondrial membrane-mitochondrial localization signal (IMM-MLS). In some implementations, when the expression vector is expressed, the luciferase protein is localized to the cytosol. In some cases, the IMM-MLS comprises a leading sequence from a mitochondrial inner membrane protein selected from ABCB10, ABCB140, Cytochrome C, and renal outer medullary potassium channel (ROMK).

The invention discloses an application of a transcription factor ChZF7 of a basophilic microalgae Chlorella sp. BLD in regulation and control of salt tolerance of microalgae. According to the invention, the coding gene of the transcription factor ChZF7 is transferred into chlamydomonas reinhardtii by using a transgenic technology after intron insertion and codon optimization, so that transgenic chlamydomonas reinhardtii is obtained. The salt tolerance of the chlamydomonas reinhardtii with the over-expressed ChZF7 gene is remarkably improved, the accumulation of neutral fat and active oxygen under the stress of 225mM salt is less than that of the chlamydomonas reinhardtii in a control group, the content of penetrating substance proline is increased, in addition, the change of cell components in the chlamydomonas reinhardtii under the stress of salt can be weakened by the over-expressed ChZF7 gene, and the salt tolerance of the chlamydomonas reinhardtii is improved. The transcription factor ChZF7 and the gene coded by the transcription factor ChZF7 can regulate and control the salt tolerance of the chlamydomonas reinhardtii, a foundation is laid for genetic improvement and directed molecular breeding of the microalgae, the transcription factor ChZF7 plays an important role in improvement of the salt tolerance of the microalgae, reutilization of salinized water resources is facilitated, and the transcription factor ChZF7 and the gene coded by the transcription factor ChZF7 have wide application prospects.

Provided is a polypeptide combination, which comprises a Rubisco polypeptide from a species of the family Chlamydomonadaceae, and a fusion polypeptide comprising an EPYC1 polypeptide of a species of the family Chlamydomonadaceae and a mineralizing peptide, wherein the mineralizing peptide can convert a metal-containing compound into a compound capable of converting light energy into chemical energy, such as a metal oxide. Further provided are a polynucleotide or a vector encoding the polypeptide combination, a microorganism containing the polypeptide combination, the polynucleotide or the vector, and a method of using the microorganism.

Provided herein are triglyceride oil compositions enriched in 1,3-dioleoyl-2-palmitoylglycerol (OPO) for use in food applications and infant nutrition formulations. Further provided herein are methods of producing non-naturally occurring triglyceride oil compositions enriched in OPO from non-naturally occurring microorganisms and applications thereof in human and animal food applications, infant formulations, and nutritional supplements.

Provided are sequence variants and fusion proteins of the photosensitive ion channel protein ChRmine and their use in treating retinal neurodegenerative diseases. These ChRmine variants and fusion proteins exhibit improved protein properties and enhanced photoresponsive characteristics compared with the wildtype ChRmine, therefore may provide effective optogenetic tools to improve the therapeutic treatment of the retinal neurodegenerative diseases.

Provided herein are stoichiometric Gas Vesicle Expression Systems (GVES), sets of polynucleotide constructs, and related vectors, cells, compositions, and methods configured for robust expression of Gas Vesicle Gene Clusters (GVGCs) in mammalian cells, particularly primary and immune cells. The GVES comprises distinct gene modules for the primary structural protein (gvpA / B) and assembly factors (AF1, AF2), operably configured to achieve a stoichiometric expression ratio, quantified by Dosage Index (DI), wherein the gvpA / B module is expressed at least 2-fold higher than the AF1 and AF2 modules.

The present invention addresses the issue of providing a modified photoreceptive chloride channel that has a narrow wavelength sensitivity range, has both short τon and short τoff, and has excellent photoreactivity. The solution is a polypeptide in which a region from the fourth transmembrane domain to the sixth transmembrane domain, counted from an N terminal-side of a Guillardia theta-derived photoreceptive chloride channel-1 (GtACR1), is replaced by a region corresponding to a Guillardia theta-derived photoreceptive chloride channel-2 (GtACR2).

The present invention discloses a heat shock transcription factor for increasing the content of fucoxanthin in Phaeodactylum tricornutum bd1650 gene and its application, characterized in that bd1650 the nucleotide sequence of the gene is as shown in SEQ ID NO: 1, and the amino acid sequence of the protein encoded by bd1650 is as shown in SEQ ID NO: 2. By knocking out the bd1650 gene in Phaeodactylum tricornutum, the content of fucoxanthin synthesized by it is increased. The advantage is that the content of fucoxanthin in Phaeodactylum tricornutum can be significantly increased.

The invention discloses a method for promoting plant growth and increasing yield through optogenetics, and belongs to the technical field of plant growth and development research. By activating light-operated hydrogen ion pump PPR protein, plant growth can be promoted, and the yield can be increased. By transferring the light-operated hydrogen ion pump PPR protein, the plant can grow quickly under the same growth condition. The growth promotion mechanism does not depend on frequent stimulation of external conditions, but accelerates a series of key physiological activities, such as a photosynthetic phosphorylation process, substance transmembrane transport and the like, regulated by hydrogen ions in plants by means of illumination, so that the plant growth efficiency is remarkably improved. And the growth target of the plant can be realized in a shorter time.

The present invention relates to a forebrain assembloid that exhibits maturity similar to that of the human brain and expresses ChR2 to enable optogenetic analysis and, more specifically, to: a method for producing a forebrain assembloid that enables optogenetic analysis; a forebrain assembloid produced by the method; and a forebrain assembloid having a single rosette structure, comprising six cortical layers and cavities, wherein the cortical layers include glial cells and uniformly express ChR2.

Methods and systems for extracting phycobiliproteins from seaweed, such as phycoerythrin, are generally described. In some embodiments, the phycobiliprotein extracted from seaweed is phycoerythrin, and the methods and systems described by this disclosure may extract phycoerythrin from seaweed while maintaining other compounds within the seaweed, such as bromoform.

The invention relates to a process for preparing a cyanobacterium extract, preferably spirulina, comprising phycocyanin enriched in trace elements. The invention also relates to an extract obtained by the process according to the invention, a composition comprising said extract and its use. Figure 1

The present invention relates to acidic compositions, in particular acidic food compositions, comprising at least one phycocyanin that is resistant to pH acid.