12 results about "Channelrhodopsin" patented technology

The invention relates to the technical field of biomedicine, discloses mutant photo-induced ion channel ChR-2 protein, and further discloses a nucleic acid construct, an expression vector, a cell, related application and a computer model. The light-sensitive channel ChR2 protein mutant obtained by the invention has stronger light current, and the light-sensitive capability of the light-sensitive channel ChR2 protein mutant is at least improved by 100 times; besides, the invention also obtains a nucleotide sequence for coding the light-sensitive channel ChR2 protein mutant, constructs a recombinant expression vector, and obtains the light-sensitive channel ChR2 protein with higher expression quantity and stronger light sensitivity, and the light-sensitive channel ChR2 protein is very suitable for expression in cells of mammals (especially human); according to the invention, the rhodopsin in different channels is systematically studied by modifying the position G224 in the helix 6 of seven transmembrane helix motifs, which proves that the mutation of the position G224 in the helix 6 in WT ChR2 accelerates the photosensitivity of the channels, and the considered position is homologous in the helix 6 of the rhodopsin in different channels.

The present disclosure is directed to compositions comprising mitochondrial optogenetics-based gene therapies and their methods of use. In some embodiments, a composition described herein comprises an expression vector comprising a first nucleic acid sequence encoding a channelrhodopsin fusion protein and a second nucleic acid sequence encoding a luciferase protein. In some cases, the first nucleic acid sequence and the second nucleic acid sequence are operably linked to an expression control sequence. In some instances, the channelrhodopsin fusion protein comprises a channelrhodopsin protein linked to an inner mitochondrial membrane-mitochondrial localization signal (IMM-MLS). In some implementations, when the expression vector is expressed, the luciferase protein is localized to the cytosol. In some cases, the IMM-MLS comprises a leading sequence from a mitochondrial inner membrane protein selected from ABCB10, ABCB140, Cytochrome C, and renal outer medullary potassium channel (ROMK).

The application provides a novel light-activated channel protein VR3.0 and uses thereof. The light-activated channel protein VR3.0 series provided in this application has a wide range of photosensitive wavelengths, higher sensitivity to light response, faster photoresponse kinetics, maintaining stable photocurrent signals under high-frequency stimulation while exhibiting good response amplitude and frequency under light stimulation of various wavelengths (especially white light, natural light). The light-activated channel protein VR3.0 series provided in this application has a clear therapeutic effect on retinal photoreceptor cell degenerative diseases, and can be used to prepare drugs for restoring the photoreceptor function of the retina, restoring the visual or photosensitive ability of subjects, and treating retinal degenerative diseases.

Provided herein are, inter alia, methods and compositions for transfecting a brain cell (e.g., neuron). The methods for transfecting a brain cell provided herein including embodiments thereof include, for example, transfecting the brain cell with a viral vector encoding a potassium-selective light-sensitive protein (e.g., kalium channelrhodopsin). Also provided herein are, inter alia, methods of modulating a neural network of brain cells (e.g., neurons) by activating a potassium-selective channelrhodopsin with light. The methods and compositions provided herein are, inter alia, useful for treating neurological or psychiatric diseases or disorders (e.g., epilepsies, Parkinson's disease, Alzheimer's, etc.).

The present disclosure provides channelrhodopsin variants that are ultrapotent optogenetic actuators of cellular electrical activity. These variants show significantly increased photocurrent magnitude and light sensitivity compared to channelrhodopsin ChRmine (SEQ ID NO:1) from Rhodomonas lens.

The invention discloses a wireless visual cortex reconstruction method and device based on optogenetics. The method comprises the steps that selective expression of red-shift light-sensitive channel protein is limited to neurons of a primary visual cortex V1 area; establishing an optical channel in a V1 area of the primary visual cortex corresponding to the skull; environment visual information is wirelessly collected, and nerve mapping conversion is carried out; red light stimulation is projected to the V1 area of the primary visual cortex through an optical channel by adopting a time sequence framing regulation and control strategy; a dynamic safety regulation and control mechanism is implemented, and photostimulation parameters are adjusted in real time according to physiological response and physical parameters. Through cooperation of V1 area red-shift light-sensitive channel protein expression, optical channel establishment, accurate nerve mapping conversion, time sequence framing red light stimulation and dynamic regulation and control, wireless visual reconstruction which does not need electronic device implantation, has single-neuron-level resolution and is safe and stable for a long time is provided for a completely blind patient.

A quantum dot (QD)-rhodopsin bioconjugate system uses Förster resonance energy transfer (FRET)-mediated induction of cellular membrane depolarization via optical activation of ion channel proteins channelrhodopsin (ChR).

To provide artificial expression constructs that selectively drive protein expression.SOLUTION: An artificial expression construct comprising a specific enhancer, a promoter, and a heterologous coding sequence, wherein in one aspect the heterologous coding sequence encodes an effector element or an expressible element, wherein the effector element comprises a reporter protein or a functional molecule, wherein the reporter protein comprises a fluorescent protein, An artificial expression construct is provided wherein the functional molecule comprises a functional ion transporter, an enzyme, a transcription factor, a receptor, a membrane protein, a cellular transport protein, a signaling molecule, a neurotransmitter, a calcium reporter, a channelrhodopsin, a CRISPR / CAS molecule, an editase, a guide RNA molecule, a microRNA, a homologous recombination donor cassette, or a designer receptor exclusively activated with a designer drug.SELECTED DRAWING: Figure 1

The present invention relates to a mutant ion channel capable of being activated by light (‘light-activated’ ion channel) and having improved properties, nucleic acids and expression vectors encoding the mutant ion channel, cells comprising such nucleic acid or expression vector, devices containing the mutant ion channel, nucleic acid or expression vector as well as respective uses and methods.

The application discloses a culture, light stimulation and signal processing method of a three-dimensional on-chip brain. The three-dimensional on-chip brain is transfected by an adeno-associated virus carrying a light-sensitive channel protein which can be specifically expressed, so that the cells of the three-dimensional on-chip brain can express the light-sensitive channel protein on the cell membrane. Blue light with a wavelength of 470 nm is emitted by a light source and irradiated on the three-dimensional on-chip brain expressing the light-sensitive channel protein, so that the light-sensitive channel protein on the cell membrane is opened, ions in the culture medium enter the cells, the ion concentration in the environment is transiently changed, the change of the ions can be detected by a multi-electrode array, the range of the change is analyzed, and the physiological characteristics of the three-dimensional on-chip brain can be obtained, the functional network structure of the three-dimensional on-chip brain can be analyzed, and the biological intelligent function of the three-dimensional on-chip brain can be revealed.

Provided is a modified channelrhodopsin having high ion permeability (photoreactivity). The solution is to replace the third extracellular domain from the N-terminal side of the three extracellular domains possessed by channelrhodopsin derived from Volvox with the corresponding extracellular domain of channelrhodopsin-2 derived from Chlamydomonas reinhardtii.