5 results about "Nerve stem cell" patented technology

The application discloses a neurodegenerative disease treatment composition and relates to the technical field of neurodegenerative disease treatment.The active ingredients of the composition include: exosomes secreted by neural stem cells, exosomes from ginseng, and exosomes from intestinal probiotics; wherein: the three kinds of exosomes exist in parallel, and are all formed by the common induction of traditional Chinese medicine active substance ginseng exosomes and intestinal probiotic fermentation liquor; after the neural stem cell exosomes are induced by the common induction of ginseng exosomes and intestinal probiotic fermentation liquor, the neurotrophic factors and regulatory RNA carried by the neural stem cell exosomes are both enhanced in content and functional activity, so that the neural repair and regulation capabilities are improved. The neural stem cell exosomes are induced by the common induction of ginseng exosomes and probiotic fermentation liquor, the neural repair, anti-inflammatory and antioxidant, and brain-gut axis regulation capabilities of the neural stem cell exosomes are enhanced. When the combined drug or stem cells are applied, the treatment effect and cell integration efficiency are improved, and a new scheme of synergistic precise treatment is provided for neurodegenerative diseases.

The invention discloses a culture medium and a method for obtaining an induced cortical-like neuron precursor cell cluster from iPSCs (induced pluripotent stem cells), and belongs to the technical field of biology. The culture medium comprises a neural stem cell induction liquid I and a neural stem cell induction liquid II, a single-layer adherent induction method is adopted, and iPSCs are efficiently differentiated into an induced cortical neuron precursor cell cluster through optimized inoculation density, matrix treatment and a staged induction process. The neural stem cells comprise neural stem cells, transition-state nerve cells and differentiated mature nerve cells (the cell type composition is highly similar to that of tissue-derived neural stem cells). The method overcomes the problems of complex induction process and high cell heterogeneity in a traditional embryoid body method, the induction efficiency can reach 100%, single cell sequencing analysis shows that all cells are nerve cells, the purity is 100%, and non-target cells are not detected. A uniform, stable and efficient cell tool is provided for brain development research, nervous system disease modeling and cell therapy.

The utility model discloses a chemical plant applied to neural stem cell transplantation extraction, including equipment frame and centrifugal extractor, is provided with the water bath heating mechanism that is heated for centrifugal extractor on equipment frame, and equipment frame includes support frame and controller, and water bath heating mechanism includes water bath heating basin, heating wire, heat insulation plugboard, second motor and screw rod, and water bath heating basin is installed on support frame and is slidably connected with it, and heating wire is fixedly installed in water bath heating basin, and heat insulation plugboard is detachably installed in water bath heating basin, and screw rod is fixedly installed on the output shaft of second motor, and the left and right ends of water bath heating basin are provided with the ear plate that cooperates with screw rod. The utility model has the advantages of water bath heating and independent temperature control, and the problems of low heating efficiency, inaccurate temperature control and inconvenient operation of the existing centrifugal extraction equipment are solved.

The present application provides a high-efficiency, safe and clinical-grade standard neural stem cell preparation method, which realizes the fate reprogramming and directional differentiation of cells under serum-free culture conditions by screening and optimizing specific small molecule compound combinations, and induces the obtainment of neural stem cells with high proliferation capacity and multi-directional differentiation potential. The method not only significantly improves the induction efficiency and quality of neural stem cells, but also avoids the influence of serum batch difference on experimental results in traditional methods, reduces production cost, and provides reliable technical support for clinical application. By transplanting the neural stem cells into the damaged area of cerebral palsy patients, the reconstruction of neural circuit and functional recovery can be promoted, and the motor ability and life quality of the patients can be improved. The serum-free clinical-grade neural stem cell preparation method and its application in cerebral palsy rehabilitation provided by the present application have the characteristics of high efficiency, stability and clinical applicability, and provide a new solution for central nervous system injury repair.

Embodiments disclose systems and apparatus that provide neurogenesis activation by FUS stimulation including activating quiescent NSCs in the subventricular zone of the lateral ventricles and promoting the proliferation of NSCs and subsequent differentiation into newly regenerated neurons. Also disclosed is a conventional MRI guided tFUS system programmed for use with ultrasonic activation of NSCs. A wireless electronic driving configuration for transcranial use under MRI guidance is provided and a cylindrically distributed ultrasound transducer array design to provide increased steering range in at least two axes. A close integration of tFUS with MRI navigation, achieved by a novel wearable thin-film MRI coil array tightly integrated with the transducer array, enables full navigation performance of MRI in real time during FUS treatment.