13 results about "Nerve stem cell" patented technology

The application discloses a neurodegenerative disease treatment composition and relates to the technical field of neurodegenerative disease treatment.The active ingredients of the composition include: exosomes secreted by neural stem cells, exosomes from ginseng, and exosomes from intestinal probiotics; wherein: the three kinds of exosomes exist in parallel, and are all formed by the common induction of traditional Chinese medicine active substance ginseng exosomes and intestinal probiotic fermentation liquor; after the neural stem cell exosomes are induced by the common induction of ginseng exosomes and intestinal probiotic fermentation liquor, the neurotrophic factors and regulatory RNA carried by the neural stem cell exosomes are both enhanced in content and functional activity, so that the neural repair and regulation capabilities are improved. The neural stem cell exosomes are induced by the common induction of ginseng exosomes and probiotic fermentation liquor, the neural repair, anti-inflammatory and antioxidant, and brain-gut axis regulation capabilities of the neural stem cell exosomes are enhanced. When the combined drug or stem cells are applied, the treatment effect and cell integration efficiency are improved, and a new scheme of synergistic precise treatment is provided for neurodegenerative diseases.

The invention belongs to the technical field of biomedical engineering materials, and provides a fibroin microsphere for nerve repair as well as a preparation method and application of the fibroin microsphere. Degummed silk is dissolved and dialyzed, and a silk protein solution is obtained; preparing a film from the silk protein solution and a film-forming agent, and dissolving in an ethanol solution to obtain silk protein microspheres; preparing a film from the silk protein microspheres and a dispersing agent; clamping between two electrode plates, and electrifying for polarization to obtain a polarized film; and dissolving the polarized film in water to obtain the fibroin piezoelectric microspheres. The fibroin piezoelectric microsphere can be used for three-dimensional culture of cells, can also be used as a tissue engineering scaffold, and is applied to induction of neural stem cell differentiation, restoration of nerve injury and treatment of neurodegenerative diseases.

The utility model discloses a culture kit for neural stem cell amplification, which comprises a kit body, a plurality of culture chambers with top openings are arranged in the kit body, a culture tube is inserted into each culture chamber, and a heating mechanism for conveying hot air to each culture chamber is arranged at one end of the kit body; the upper end of the culture tube is provided with an extension part extending outwards, the extension part is arranged on the box body, the diameter of an opening in the top of the culture chamber is larger than that of the culture tube, two symmetrical storage grooves are formed in the opening of the culture chamber, elastic clamping pieces are arranged in the two storage grooves and used for clamping the upper portion of the culture tube, and a swing mechanism is arranged at the bottom of the culture chamber. The utility model relates to a culture tube swinging device. Hot air is conveyed to each culture room through the heating mechanism, and independent temperature control can be performed on each culture room, so that a plurality of groups of culture tubes can be conveniently compared.

The invention relates to the compound (2S,3S,4R,5R,6S,8R,9S,11R,15R)-3,5,15- triacetoxy-6(17)-epoxy-8-hydroxylathyrane-12E-en-14-one, also called (8R)-8-hydroxyepoxyboetirane A, as well as to a method for obtaining same and the use thereof to promote neuronal differentiation from neural precursors or neural stem cells in culture and in brain lesions. The invention also relates to the use of this compound to prepare a pharmaceutical composition that can be used to treat central nervous system lesions associated with neuron loss.

The invention belongs to the technical field of cells, and particularly relates to a neural stem cell culture medium and a culture method. The neural stem cell culture medium comprises a basic culture medium and the following components added into the basic culture medium in terms of final concentration: 0.5-10 mg / L of human insulin, 20-30 [mu] g / L of epidermal growth factors, 2-3 [mu] g / L of folic acid, 2-5 [mu] g / L of N-glutamine, 10-40 [mu] g / L of N-acetylcysteine, 0.1-5 mg / L of ethanolamine, 0.5-2 mg / L of carnitine, 0.5-5 mg / L of vitexin glucoside and 15-25 [mu] g / L of plecanatide. The vitexin glucoside and the plecanatide play a synergistic effect, so that the rapid division and proliferation of the neural stem cells are remarkably promoted, the cell differentiation potential of the neural stem cells is maintained, and the clinical application of the neural stem cells can be fully guaranteed.

The invention discloses a culture medium and a method for obtaining an induced cortical-like neuron precursor cell cluster from iPSCs (induced pluripotent stem cells), and belongs to the technical field of biology. The culture medium comprises a neural stem cell induction liquid I and a neural stem cell induction liquid II, a single-layer adherent induction method is adopted, and iPSCs are efficiently differentiated into an induced cortical neuron precursor cell cluster through optimized inoculation density, matrix treatment and a staged induction process. The neural stem cells comprise neural stem cells, transition-state nerve cells and differentiated mature nerve cells (the cell type composition is highly similar to that of tissue-derived neural stem cells). The method overcomes the problems of complex induction process and high cell heterogeneity in a traditional embryoid body method, the induction efficiency can reach 100%, single cell sequencing analysis shows that all cells are nerve cells, the purity is 100%, and non-target cells are not detected. A uniform, stable and efficient cell tool is provided for brain development research, nervous system disease modeling and cell therapy.

The present invention relates to a method for converting human fibroblasts into neural stem cells, and more particularly, to a method for converting human fibroblasts into neural stem cells by direct transdifferentiation using sendai virus, mRNA of stem cell-related factors, or a combination of miRNA and a small molecule compound, and to a use thereof. According to the present invention, high-quality neural stem cells can be induced in a short period of time from human fibroblasts by direct transdifferentiation, so that a sufficient amount of cells can be obtained for cell therapy without side effects of tumor formation, and thus the neural stem cells can be used as a cell therapeutic agent for brain diseases.

The utility model discloses a chemical plant applied to neural stem cell transplantation extraction, including equipment frame and centrifugal extractor, is provided with the water bath heating mechanism that is heated for centrifugal extractor on equipment frame, and equipment frame includes support frame and controller, and water bath heating mechanism includes water bath heating basin, heating wire, heat insulation plugboard, second motor and screw rod, and water bath heating basin is installed on support frame and is slidably connected with it, and heating wire is fixedly installed in water bath heating basin, and heat insulation plugboard is detachably installed in water bath heating basin, and screw rod is fixedly installed on the output shaft of second motor, and the left and right ends of water bath heating basin are provided with the ear plate that cooperates with screw rod. The utility model has the advantages of water bath heating and independent temperature control, and the problems of low heating efficiency, inaccurate temperature control and inconvenient operation of the existing centrifugal extraction equipment are solved.

The invention discloses a preparation method and application of neural stem cells based on chemical reprogramming. The preparation method comprises the following steps: performing three-stage reprogramming on human umbilical cord mesenchymal stem cells by using a specific chemical small molecule composition to obtain chemically induced pluripotent stem cells, and further performing induced differentiation to obtain neural stem cells. The preparation method is high in efficiency, good in stability, wide in raw material source and easy to produce and popularize, the prepared neural stem cells are free of tumorigenicity and can be differentiated into mature neurons with electrophysiological activity in vitro, a potential cell treatment source is provided for nervous system diseases, medicine delivery can be carried out in an intranasal administration mode and the like, and the application prospect is wide. And a new technical path is provided for clinical treatment of nervous system diseases.

The present application provides a high-efficiency, safe and clinical-grade standard neural stem cell preparation method, which realizes the fate reprogramming and directional differentiation of cells under serum-free culture conditions by screening and optimizing specific small molecule compound combinations, and induces the obtainment of neural stem cells with high proliferation capacity and multi-directional differentiation potential. The method not only significantly improves the induction efficiency and quality of neural stem cells, but also avoids the influence of serum batch difference on experimental results in traditional methods, reduces production cost, and provides reliable technical support for clinical application. By transplanting the neural stem cells into the damaged area of cerebral palsy patients, the reconstruction of neural circuit and functional recovery can be promoted, and the motor ability and life quality of the patients can be improved. The serum-free clinical-grade neural stem cell preparation method and its application in cerebral palsy rehabilitation provided by the present application have the characteristics of high efficiency, stability and clinical applicability, and provide a new solution for central nervous system injury repair.

The present invention discloses a zinc-based nano-organic framework coating material loaded with microRNA and its application, belonging to the technical field of biomedicine. The zinc-based nano-organic framework coating material loaded with microRNA includes a zinc-based nano-organic framework material; microRNA-34 is loaded on the zinc-based nano-organic framework material; the surface of the zinc-based nano-organic framework material is coated with the cell membrane of neural stem cells. In the present invention, neural stem cells endocytose miR-34@ZIF-8-SCM to induce the differentiation of neural stem cells into neurons; the cell membrane of neural stem cells not only has homology with neural stem cells, but also increases the release rate of microRNA-34 by miR-34@ZIF-8-SCM, further increasing the differentiation rate of neural stem cells into neurons.

The present invention discloses a protein marker for detecting or evaluating paracrine secretion of human neural stem cells, a screening method and application thereof, wherein the protein marker is selected from NCAN, LAMB2, MMP2, SFRP1, RTN4, B4GAT1, NPY, VCAN, GPC2, CHL1, SERPINE2, PTPRF, PTN, LDLR, NRP2, NLGN3, CNTN2, C1QL1, NLGN4X, VLDLR, etc. The screening method comprises: extracting total protein from human neural stem cell samples for proteomic analysis and transcriptomic analysis with reference genomes, obtaining proteins with a common protein abundance expression at the front and a positive regulatory function through data processing and comparative screening, and detecting by ELISA enzyme-linked immunosorbent assay. The expression level of the protein marker is detected for detecting or evaluating paracrine secretion of human neural stem cells, and the application value is great.

Embodiments disclose systems and apparatus that provide neurogenesis activation by FUS stimulation including activating quiescent NSCs in the subventricular zone of the lateral ventricles and promoting the proliferation of NSCs and subsequent differentiation into newly regenerated neurons. Also disclosed is a conventional MRI guided tFUS system programmed for use with ultrasonic activation of NSCs. A wireless electronic driving configuration for transcranial use under MRI guidance is provided and a cylindrically distributed ultrasound transducer array design to provide increased steering range in at least two axes. A close integration of tFUS with MRI navigation, achieved by a novel wearable thin-film MRI coil array tightly integrated with the transducer array, enables full navigation performance of MRI in real time during FUS treatment.