92 results about "Cortisone" patented technology

The present invention discloses a complete medium for culturing DF-1 cells, including DMEM basal medium 13.7g / L, insulin, non-essential amino acids, transferrin, glutathione, ascorbyl phosphate, sodium selenite, ethanolamine, hydrocortisone, and newborn calf serum. Wherein non-essential amino acids include glycine, alanine, proline, serine, and asparagine. A complete medium for culturing DF-1 cells provided by the present invention can well promote the proliferation of DF-1 cells, can achieve the same proliferation effect as high-concentration fetal bovine serum, and is suitable for the adherent culture of DF-1 cells; using low-concentration newborn calf serum, it is possible to greatly avoid the batch differences, unstable culture results, cytotoxicity, and high risk of carrying pathogens brought by the culture medium with high-concentration serum, narrow application range, expensive fetal bovine serum, and high culture cost; the components are simple, the culture medium preparation process is short in time consumption, the culture efficiency is high, the repeatability is good, and it is suitable for batch production.

The invention discloses a progesterone 5beta-reductase mutant and application thereof in synthesis of 5beta-dihydrosteroid, relates to the technical field of biology, and in particular relates to application of gene mining and engineering modification of progesterone 5beta-reductase derived from bacteria in asymmetric synthesis of 5beta-dihydrosteroid. According to the progesterone 5 beta-reductase LpP5beta R disclosed by the invention, the progesterone 5 beta-reductase LpP5beta R from bacteria psoromais is obtained by a gene mining method, and the progesterone 5 beta-reductase LpP5beta R can be subjected to soluble expression in escherichia coli. The mutant obtained by mutating the wild type LpP5beta R has obviously improved catalytic ability on progesterone, and can efficiently catalyze delta 4-3-carbonyl steroids such as hydrocortisone, dinorcitol and the like to generate corresponding 5beta-dihydrosteroids. According to the invention, the technical bottlenecks of poor heterologous expression and low catalytic efficiency of plant-derived progesterone 5beta-reductase are solved, and a potential biocatalyst is provided for green manufacturing of 5beta-dihydrosteroid drugs.

The invention relates to the technical field of medical detection, in particular to a method for detecting aldosterone, cortisol, deoxycorticosterone and cortisone in plasma through LC-MS / MS. The method comprises the following steps: (a) mixing a plasma sample with an internal standard solution to obtain a mixed solution; (b) adding the mixed solution into the magnetic bead suspension, sequentially carrying out adsorption, weak washing, strong washing and elution, and collecting an eluent; (c) carrying out liquid nitrogen blowing and redissolving on the eluent, and then carrying out LC-MS / MS detection; wherein the surfaces of the magnetic beads are modified with steroid adsorption groups, and the target detection objects are aldosterone, cortisol, deoxycorticosterone and cortisone. The detection method is convenient and simple, the detection efficiency is higher than that of a traditional method, and a feasible solution is provided for promoting popularization and application of the LC-MS / MS technology in clinical practice.

This disclosure provides a method for treating hair loss in a subject requiring treatment for hair loss, comprising topically administering an effective amount of cortexolone-17α-propionate and an effective amount of minoxidil to the subject. This disclosure further provides a topical pharmaceutical formulation comprising cortexolone-17α-propionate, minoxidil, and one or more pharmaceutically acceptable carriers.

The invention discloses a preparation method of fluorohydrocortisone acetate, and belongs to the technical field of medicine synthesis. According to the method, the fluorine hydrocortisone acetate is obtained through one-step reaction by taking the Anecortisone acetate as an initial raw material and taking a mixture of an organic solvent and water as a solvent under the condition of a Fe (III) reagent and a fluorine reagent. The preparation method disclosed by the invention is simple and convenient to operate, high in safety, mild in reaction condition, low in cost, high in yield, small in corrosivity and suitable for industrial mass production; meanwhile, the problems that in the prior art, operation is inconvenient, corrosivity is high, toxicity is large, dangerousness is high, the requirement for equipment is high, and the yield is low are solved, and a new technical scheme more suitable for industrial production is provided.

The invention discloses a method for establishing a small animal dilation line model. Comprising the following steps that an incision is formed in the tail portion of the back skin of a rat, a subcutaneous bag is created below the corium layer of the incision through tweezers, a tissue expander is placed in the incision and close to the position close to the neck and above the vertebra, the subcutaneous bag is closed, and the incision is sewed and closed; smearing the hydrocortisone ointment every day to uniformly cover the skin of the modeling part, and continuously intervening for 3 days; the smearing part is away from the incision part; normal saline is injected into the tissue dilator every day; and taking out the tissue expander after 21 days, standing for 7 days, observing the back condition of the rat, and when the characteristics conforming to early red lines of dilatation lines are observed, indicating that modeling succeeds. The purpose of stretching the skin is achieved by implanting the tissue expander in a small animal body and increasing the size of the tissue expander along with time, and the expansion line generation condition is simulated. The method is simple, convenient to operate and high in success rate.

The present disclosure provides methods of treating skin acne-like rash in a subject in need thereof. The method comprises applying an effective amount of picosterone-17 alpha-propionate to the surface of the subject. The acne-like rash may include papules, pustules, nodules, scabs, cysts, or any combination thereof on an affected area of the subject. An effective amount of the picosterone-17 [alpha]-propionate can be administered in a surface pharmaceutical formulation comprising the picosterone-17 [alpha]-propionate and one or more pharmaceutically acceptable carriers.

The invention provides a 3-hydroxypyrone derivative as well as a preparation method and application thereof, the compound can effectively inhibit release of proinflammatory cytokines in lipopolysaccharide-induced microglial cells, inhibit release of NO by the lipopolysaccharide-induced microglial cells, inhibit apoptosis of polysaccharide-induced microglial cells, and inhibit apoptosis of lipopolysaccharide-induced microglial cells. The compound has excellent anti-neuroinflammation activity and neuroprotective effect, the activity of the compound is superior to that of positive controls such as curcumin and hydrocortisone, and the compound can be used for preventing and / or treating neuroinflammation and neurodegenerative diseases.

The invention discloses a construction method of a pulmonary arterial hypertension traditional Chinese medicine syndrome model, and aims to simulate a pulmonary arterial hypertension animal model with syndrome characteristics of heart-kidney yang deficiency. The method comprises the following steps: inducing rat pulmonary arterial hypertension pathological basis by adopting monocrotaline, and inducing a heart-kidney yang deficiency state in combination with hydrocortisone intervention from the 15th day; the characteristics of the model are evaluated through multiple indexes such as echocardiography, blood gas analysis, tri-gland axis hormone, energy metabolism, renal functions, biochemical factors, HE staining and ultrastructure observation. Results show that the model has pathological changes of pulmonary arterial hypertension and syndromes such as yang deficiency, water retention, life gate fire decline and the like in traditional Chinese medicine, the construction method is stable and repeatable, and the model is suitable for an experimental platform for traditional Chinese medicine intervention research and combination of traditional Chinese medicine and western medicine.

The invention provides a special culture medium for prostate cancer organoid and a culture method thereof, and belongs to the technical field of biological medicines. The special culture medium for the prostatic cancer organoid is composed of a basic culture medium and a culture additive, and the basic culture medium does not contain exogenously added Noggin recombinant protein and R-spondin recombinant protein. The culture additive is prepared from a basic fibroblast growth factor, a keratinocyte growth factor, a fibroblast growth factor 10, insulin, a p38MAPK signal channel inhibitor, an insulin-like growth factor 1, B27, nicotinamide, N-acetylcysteine, hydrocortisone, a ROCK kinase inhibitor, retinoic acid, interleukin 6, prostaglandin E2, a eriocin and androgen. According to the special culture medium for the prostatic cancer organoid, the culture success rate is increased while the cost is reduced.

The invention discloses application of phosphatidylserine-hydrocortisone microvesicles in preparation of a medicine for treating microvascular regeneration after cerebral apoplexy, and relates to the technical field of biomedicine. According to the microbubble, the blood brain barrier can be accurately opened through the ultrasonic vacuolation technology, the medicine can be delivered in a targeted mode, nerve protection and function improvement are accurately evaluated through ultrasonic super-resolution micro blood flow imaging, and microvessel regeneration is accurately regulated and controlled based on H3K18 lactic acid modification.

The application relates to the technical field of animal model construction, in particular to a construction method and application of a spleen-kidney yang deficiency syndrome ovarian reserve function decline animal model. The construction method comprises the following steps: a model group animal is subjected to modeling by adopting the method of senna leaf water decoction gavage and hydrocortisone and cyclophosphamide injection, and a spleen-kidney yang deficiency syndrome ovarian reserve function decline animal model is obtained. In the application, the three of senna leaf, hydrocortisone and cyclophosphamide are used in synergy to administer the animal model, and the complex syndrome of the clinical spleen-kidney yang deficiency type DOR is accurately reproduced, and modern scientific basis is provided for the theories of traditional Chinese medicine such as the same treatment for different diseases.

The invention discloses a system and a method for detecting the level of cortisol in urine and application, and relates to the technical field of detection of the level of cortisol in urine. The detection system comprises a 24h urocortisol related data and urocortisol correlation analysis module. Wherein the 24-hour urocortisol related data and urocortisol correlation analysis module is based on a monitored 24-hour urocortisol level value after a patient takes hydrocortisone acetate, a 24-hour urocortisol related data level value before and 1 hour after the patient takes hydrocortisone acetate at 8 o'clock in the morning, and a 24-hour urocortisol related data level value before and 1 hour after the patient takes hydrocortisone acetate at 4 o'clock in the afternoon; and performing multivariate linear correlation analysis to obtain a linear relation. The urine cortisol level is calculated according to the linear relational expression, the treatment effect of hormone drugs, especially hydrocortisone acetate, on hypopituitary function can be accurately monitored, and the phenomenon that in the prior art, all urine within 24 h is collected and then cortisol detection is conducted, so that the result is not accurate is avoided.

The invention relates to a culture method and a culture medium for reducing the number of differential transcripts of epithelial organoid and in-vivo tissues, and solves the technical problems of low growth rate and low formation rate of the organoid in the prior art. The basic components of the culture medium comprise a DMEM / F12 basal culture medium, a penicillin-streptomycin solution, a Glutamax additive, a recombinant spinal protein, a recombinant head protein, Y-27632, an epidermal growth factor, an N-2-hydroxyethyl piperazine-N-2-ethanesulfonic acid buffer solution, nicotinamide, A83-01 and CHIR99021, and the additional components of the culture medium comprise a Wnt-3A additive, an ITS-X additive and hydrocortisone. The invention also provides a culture method of the epithelial organoid. The method can be used for culturing epithelial organs.

An intranasal composition generally includes hydrocortisone, a hydrocortisone derivative, or a salt thereof. A method of administering an intranasal composition that includes hydrocortisone, a hydrocortisone derivative, or a salt thereof to a subject. A method of treating adrenal insufficiency in a subject, the method includes administering to a subject, a dose of a composition comprising hydrocortisone, a hydrocortisone derivative, or a salt thereof in an amount effective to treat adrenal insufficiency.

The invention belongs to the technical field of pharmaceutical analysis, and particularly relates to a biological analysis method for detecting the concentration of hydrocortisone butyrate in blood plasma, which is used for analyzing a compound to be detected in the blood plasma through an LC-MS / MS (Liquid Chromatography-Mass Spectrometry / Mass Spectrometry) method. According to the detection method, a liquid-liquid extraction pretreatment method is preferably adopted, finasteride serves as an internal standard substance, a Shim-pack Velox SP-C18 chromatographic column is adopted, gradient elution and electrospray ionization source tandem mass spectrometry detection are carried out, and the detection method is high in sensitivity, precise, accurate, high in analysis speed, high in sensitivity, high in sensitivity, high in sensitivity, high in sensitivity, high in sensitivity, high in sensitivity, high in sensitivity, high in sensitivity and high in sensitivity. The sample analysis requirement in the clinical research of the hydrocortisone butyrate is met.

The present disclosure provides a method of treating hair loss in a subject in need thereof, comprising topically administering to the subject an effective amount of pyrilutamide in combination with a hair growth-stimulating compound selected from the group consisting of cortexolone-17α-propionate and minoxidil. The present disclosure further provides a topical pharmaceutical formulation comprising pyrilutamide, a hair growth-stimulating compound, and one or more pharmaceutically acceptable carriers.

The present invention relates to an epithelial organoid culture medium and a culture method, which solve the technical problems of low growth rate and low formation rate of organoids in the prior art. The epithelial organoid culture medium contains basic components and additional components. The basic components include DMEM / F12 basal medium, penicillin-streptomycin solution, Glutamax additive, recombinant spondin, recombinant norrin, Y-27632, epidermal growth factor, N-2-hydroxyethylpiperazine-N-2-ethanesulfonic acid buffer solution, nicotinamide, A83-01 and CHIR99021. The additional components include Wnt-3A, ITS-X additive and hydrocortisone. The present invention also provides a culture method for epithelial organoids. The present invention can be used for the culture of epithelial organoids.

The present invention belongs to the technical field of biomedicine, and specifically relates to a nociceptor skin organoid chip and its culture medium, construction method and application. A culture medium for a nociceptor skin organoid chip, the culture medium is composed of fetal bovine serum and the following components added to the fetal bovine serum: epidermal growth factor, bovine pituitary extract, Normocin, B27, nerve growth factor, insulin, hydrocortisone, human brain-derived neurotrophic factor and capsaicin. Using this culture medium to co-culture a skin chip with nociceptors to obtain a nociceptor skin organoid chip, which can be used for the verification of pain perception effects.

This invention relates to novel compounds of the Formula (1m1) wherein R1, R2, R3, A1, Cy1 and E are as defined in the specification, and pharmaceutical compositions thereof, which are useful for the therapeutic treatment of diseases associated with the modulation or inhibition of 11β-HSD1 in mammals. The invention further relates to pharmaceutical compositions of the novel compounds for use in the reduction or control of the production of cortisol in a cell or the inhibition of the conversion of cortisone to cortisol in a cell.

Orodispersible film (ODF) is an innovative drug formulation that allows for adjustable dosing and improved patient compliance. It is administered into the mouth where it dissolves, making it suitable for children. The present invention provides an optimized ODF gel formulation and an optimal ODF containing therapeutic doses of hydrocortisone-type corticosteroids (HTC). This ODF is thin, flexible and transparent, and suitable for production in hospital pharmacy using standard equipment. The ODF of the invention allows accurate hydrocortisone levels to be released systemically in patients in need thereof, and its good physico-chemical stability is herein demonstrated for several months. This opens up new opportunities for use in pediatric patients, notably for children suffering from congenital adrenal hyperplasia.

The application discloses a steroid C14 alpha hydroxylase CLCYP14 mutant, relates to the field of proteins, and applies a site-directed saturation mutation technology and a combination mutation strategy to screen and obtain CLCYP14 mutants I111L-M115K and I111L-V124W with high hydroxylation specificity. Compared with CLCYP14, the hydroxylation selectivity and conversion rate of I111L-M115K and I111L-V124W for steroid compounds progesterone, 21-hydroxyprogesterone, 5beta-hydroxyprogesterone, 1-dehydroprogesterone, 21-hydroxypregna-1,4-diene-3,20-dione and deoxycortisone are obviously improved.

This invention relates to a method for constructing and evaluating an animal model of Alzheimer's disease characterized by deficiency and blood stasis. Based on APP / PS1 mice, the model was induced by sleep deprivation, hydrocortisone, and carrageenan to induce qi deficiency, kidney deficiency, and blood stasis, respectively, for a total modeling period of 28 days, resulting in an animal model of Alzheimer's disease with deficiency and blood stasis. This model was constructed using a multi-factor combined approach, and its symptom manifestations are consistent with clinical observations. It is simple, effective, and easy to operate, providing a reliable experimental vehicle for the development of innovative drugs and the study of mechanisms of action in Alzheimer's disease with deficiency and blood stasis.

The application provides a culture medium and a culture method of a gastric cancer organoid. The culture medium comprises an MST1 / 2 kinase inhibitor, a fibroblast growth factor 7, R-spondin 1, hydrocortisone, a B27 supplement, an N2 supplement, insulin, a dual regulatory protein, at least one Rho protein kinase inhibitor selected from Y27632, fasudil and H-1152, forskolin, gastrin, fetal bovine serum and cholera toxin. Compared with the existing culture method, the in-vitro culture using the culture medium has higher amplification efficiency; the culture of the gastric cancer organoid using the culture medium can maintain the morphological structure and pathological characteristics of the primary tissue, and improve the success rate and survival rate of the gastric cancer organoid culture.

The present application relates to a culture method and culture medium for reducing the number of epithelial organoids and in vivo tissue differential transcripts, which solves the technical problems of low growth rate and low formation rate of organoids in the prior art. The epithelial organoid culture medium contains basic components and additional components. The basic components contain DMEM / F12 basic medium, penicillin-streptomycin solution, Glutamax additive, recombinant vertebral protein, recombinant cephalopod protein, Y-27632, epidermal growth factor, N-2-hydroxyethylpiperazine-N-2-ethanesulfonic acid buffer solution, nicotinamide, A83-01 and CHIR99021. The additional components contain Wnt-3A, ITS-X additive and hydrocortisone. The present application simultaneously provides a culture method for epithelial organoids. The present application can be used for the culture of epithelial organoids.

The invention discloses a method for determining impurity content in a hydrocortisone acetate intermediate 2 by utilizing HPLC (High Performance Liquid Chromatography), and belongs to the technical field of pharmaceutical analysis. According to the method, a chromatographic column taking octadecyl bonded silica gel as a filler is adopted, water is taken as a mobile phase A, acetonitrile is taken as a mobile phase B, gradient elution is carried out, and the content of impurities in hydrocortisone acetate bromide is measured. According to the method disclosed by the invention, the hydrocortisone acetate intermediate 2 can be well separated from impurities, the spectral purity of a destroyed sample is greater than 990 through peak purity detection under various conditions, and the detection specificity of related substances is good; meanwhile, when the chromatographic condition parameters are slightly changed, the separation and inspection results of main components and impurities are not influenced, and the durability is good; according to the method, the related substances in the hydrocortisone acetate intermediate 2 can be rapidly, effectively, accurately and reliably separated and detected, the product quality of the hydrocortisone acetate intermediate 2 is improved, and the medication safety of a patient is improved.

Provided herein are oral liquid formulations of hydrocortisone. Also provided herein are methods of making and using hydrocortisone oral liquid compositions for the treatment of certain diseases including endocrine disorders, rheumatic disorders, collagen diseases, dermatologic diseases, and others.

The present invention discloses a carbonyl reductase mutant and its application in synthesizing 11β-hydroxysteroid compounds. Carbonyl reductase derived from Homo sapiens is modified by semi-rational design methods to provide a carbonyl reductase mutant. The mutant can efficiently catalyze the synthesis of 11β-hydroxysteroid compounds prednisolone, hydrocortisone and methylprednisolone using prednisone, cortisone and methylprednisolone as substrates.

This disclosure provides fast acting itch relief and cooling sprays containing hydrocortisone, a cooling agent, such as sensate molecule (e.g. menthyl lactate), and a soothing agent (such as e.g., Aloe vera). The sprays of the disclosure can be used to relieve itching associated with minor skin irritations, inflammation, and rashes due to eczema, psoriasis, poison ivy, oak, sumac, insect bites, detergents, jewelry, cosmetics, soaps, seborrheic dermatitis. The disclosure also provides for methods of treating skin with cooling sprays as well as containers containing the spray.

The invention relates to the technical field of external ointment, in particular to compound external ointment for treating leukoplakia vulvae and a preparation method of the compound external ointment. 0.03 part of tacrolimus; 2.5 parts of hypocrellin; 5 parts of a cortex phellodendri extract; 10 parts of hyaluronic acid; 3 parts of vitamin E; and 70-75 parts of a matrix. Inflammation is rapidly controlled through low-efficiency glucocorticoid, local immune abnormality is inhibited through the immunomodulator, tissue is repaired through traditional Chinese medicine components, and bacteria are resisted, so that a quick-acting and long-acting combined mode is formed, hormone side effects are neutralized by adding hyaluronic acid and vitamin E, and the feasibility of the treatment course is prolonged; the total effective rate of the combined medicine for avoiding vitamin E oxidation through low-temperature mixing is remarkably higher than that of a traditional scheme, the occurrence rate of skin atrophy is reduced to 4% and far lower than 22% of that of the traditional scheme, and meanwhile, the staged mixing process ensures that the retention rate of active ingredients is larger than 95% and is remarkably superior to that of the traditional process.