119 results about "Induced stem cells" patented technology

The invention provides a stem cell exosome vesicle induced by traditional Chinese medicine functional components as well as a preparation method and application thereof, and belongs to the technical field of biological medicines. According to the preparation method of the stem cell exosome vesicle, traditional Chinese medicine functional components serve as an exosome inducer, stem cells can be induced to proliferate and secrete exosome with the enhanced function, the yield of the exosome can be increased, bioactive molecules can be loaded, and the bioavailability of the exosome in diabetes treatment is remarkably improved. The stem cell exosome vesicle disclosed by the invention can be applied to treatment of diabetes and complications thereof, not only can improve functions of islet cells, but also can treat chronic inflammation, microangiopathy and other metabolic diseases related to diabetes, and has a wide clinical application prospect.

The present invention relates to the field of stem cell technology. The present invention discloses a method for inducing stem cells to secrete specific factors, which includes stimulating stem cells with high-frequency waves, emitting sound waves of a specific frequency to stem cells, regulating stem cells in a hypoxic environment, culturing stem cells in a hypoxic environment, stimulating stem cells with oxidative stress, and subjecting stem cells to oxidative stimulation treatment. The present invention can significantly enhance the proliferation ability of stem cells, promote the secretion of multiple active factors, and improve cell function and adaptability. As a non-invasive physical method, high-frequency wave stimulation can activate internal cell signal pathways without introducing exogenous substances, significantly improving the proliferation ability and tissue repair potential of stem cells. Hypoxic environment regulation simulates the naturally existing microenvironmental conditions in the body, greatly promoting the secretion of active factors such as vascular endothelial growth factor, basic fibroblast growth factor, and brain-derived neurotrophic factor for tissue repair and immune regulation.

The invention provides a composition for beautifying, which comprises a blood platelet concentrated solution prepared by differentiation of human pluripotent induced stem cells (iPSC), and an additive for preparing a blood platelet suspension or gel, and is characterized in that the composition for beautifying comprises the following components: a blood platelet concentrated solution prepared by differentiation of human pluripotent stem cells (iPSC), and an additive for preparing the blood platelet suspension or gel. The invention aims to provide a functional platelet-rich composition which can be prepared in a large scale, is stable in batch and can be produced in a standardized manner in the aspect of promoting wound healing. According to the composition, the platelet concentrate and platelet-rich plasma (PRP) separated from blood in the composition are completely different in component, the component is stable, and the slow-release growth factor is beneficial to tissue repair.

The invention discloses preparation and application of dual-network hydrogel. The dual-network hydrogel comprises the following components: (Z1) a spinal cord acellular matrix network DSM formed by self-assembly of a spinal cord acellular matrix dSECM; and (Z2) a metal-polyphenol network MPN formed by coordination of metal ions and polyphenols; wherein the DSM and the MPN are cross-linked through hydrogen bonds to jointly form a double-network structure. DSM provides a microenvironment suitable for cell survival and growth for the dual-network hydrogel and induces stem cells to differentiate into neurons, and MPN provides the dual-network hydrogel with biological effects of inhibiting inflammation and accumulation of reactive oxygen species (ROS). The dual-network hydrogel has the advantages of the two components, the active component neurotrophic factor and the inhibitor loaded on the dual-network hydrogel can regulate and control immune response after SCI, improve neuroinflammation microenvironment and promote nerve repair, and a new strategy is provided for in-situ tissue repair of SCI.

The invention belongs to the field of medicines, and discloses application of a stress-induced stem cell extract in treatment of cerebral apoplexy. The inventor finds that stem cells are cultured under proper stress conditions through screening, and the separated stem cell extract (also known as Alritoin) can increase the axon length and dendritic length of normal primary neurons and is beneficial to growth and conduction of nerve fibers; in a pdMCAO model, the volume of cerebral infarction in the acute stage of ischemia of a mouse can be remarkably reduced, the neurological function score can be improved, and a relatively good improvement trend is achieved in the aspect of cognition; in the acute stage of cerebral ischemia reperfusion, the pharmaceutical composition has remarkable effects on reducing the cerebral infarction volume of a mouse, reducing relative edema and improving the neurological function, and is relatively long in medication time window; in a cerebral ischemia reperfusion model, the trend of improving local cerebral blood flow is shown. The allitoin is expected to be developed into a promising drug for cerebral apoplexy, especially a drug for treating ischemic cerebral apoplexy.

The invention provides a preparation method of gas-jet mineralized collagen-hydrogel microspheres, and relates to the technical field of biological materials, and the preparation method comprises the following steps: dissolving collagen in a phosphate solution, dropwise adding the phosphate solution and a CaCl2 solution into a continuously stirred alkaline solution together, reacting for 12-48 hours, centrifugally collecting a precipitate, washing with water, centrifuging, and freeze-drying to obtain mineralized collagen; the method comprises the following steps: dissolving mineralized collagen and sodium alginate in deionized water, spraying the solution into a CaCl2 solution through a gas spraying spinning head by adopting a gas spraying technology, collecting microspheres, and repeatedly cleaning by adopting deionized water to obtain the gas spraying mineralized collagen-hydrogel microspheres. Mineralized collagen is encapsulated and loaded through microspheres, so that interferences of tissue fluid are isolated, and non-specific aggregation is reduced; optimizing the ratio of mineralized collagen, and directionally inducing stem cells to differentiate; the diameter of the microsphere is accurately controlled by regulating and controlling gas injection parameters to adapt to different wounds; active ingredients are controlled according to needs through a microsphere cross-linked network, and the multi-stage requirements of subcutaneous repair are met.

The invention discloses a cerium nano-enzyme composite active material and application thereof in hepatic failure treatment, and belongs to the technical field of nano-materials. The invention combines the functions of miR122 and cerium dioxide nano-enzyme, develops a new stem cell differentiation inducer, namely a cerium nano-enzyme composite active material, and deeply studies the potential mechanism of the nano-enzyme material with catalytic activity in regulating stem cell fate, and the cerium dioxide nano-enzyme can be used for delivering miR122 into stem cells after being modified, so that the stem cell differentiation inducer can be used for inducing stem cell differentiation. The miR122 is highly enriched in cells, so that hepatic differentiation is induced. Meanwhile, the catalytic activity of the cerium dioxide nano-enzyme can regulate the ROS steady state in the cells and protect mitochondria from being damaged by ROS induction, so that the mitochondrial function is maintained, the energy requirement in the differentiation process is met, and the method is of great importance to successful differentiation of the stem cells. In order to solve the problem of activity damage in hepatic cell transplantation, a layer of Ce < 4 + >-polyphenol skeleton is coated on the surface of induced hepatic-like cells (iHLCs), so that the function and activity of the hepatic cells after transplantation are further protected, and the in-vivo treatment effect of the hepatic cells is improved.

Provided are a reagent combination or a kit for constructing an embryoid and the use thereof. The reagent combination or the kit can be used for inducing a stem cell to produce a blastocyst-like lineage precursor cell, a blastocyst-like cell and / or an embryoid, which are based on small-molecule induction and independent of transgenes, and have a single cell source. The produced blastocyst-like lineage precursor cell and blastocyst-like cell have a full blastocyst lineage, and are seed cells having balanced developmental competence and totipotency, which solves the problem of unbalanced cell maturation in the prior art. The produced embryoid is highly similar to natural embryos in terms of morphological characteristics and transcriptomic characteristics, has the capability of highly reproducing post-implantation embryonic development and / or gastrulation, which is beneficial for research on embryonic development in vitro. The method for inducing the stem cell to produce the blastocyst-like lineage precursor cell, the blastocyst-like cell and / or the embryoid by using the reagent combination or the kit is simple, and has high applicability and high embryoid construction efficiency.

Described herein are methods of inducing proliferation of PRX1 expressing stem cells stem cells in a subject in need thereof, the method can include administering an effective amount of niclosamide, or a pharmaceutically acceptable salt, prodrug, or derivative thereof.

The present invention addresses the problem of providing novel technology for inducing differentiation of stem cells.　The present invention provides a cell differentiation induction method, said cell differentiation induction method comprising a culturing step for culturing stem cells in the presence of extracellular vesicles derived from a tissue specific cell, wherein the tissue specific cell and the cells obtained by differentiation induction are the same type of cells.

The invention provides a composition for treating alopecia, which comprises a blood platelet concentrated solution prepared by differentiation of human pluripotent induced stem cells (iPSC), and an additive for preparing a blood platelet suspension or gel. The invention aims to provide a functional platelet-rich composition which can be prepared in a large scale, is stable in batch and can be produced in a standardized manner in the aspect of treating alopecia. According to the composition, the platelet concentrate and platelet-rich plasma (PRP) separated from blood in the composition are completely different in component, the component is stable, and the slow-release growth factor is beneficial to tissue repair.

The invention provides a differential medium composition and a method for preparing mesenchymal stem cells by inducing stem cells to differentiate in a body segment direction, and belongs to the field of biology. The differential culture medium combination comprises a culture medium A, a culture medium B, a culture medium C and a culture medium D. The culture medium A comprises a basic culture medium and a first small molecule compound combination, the culture medium B comprises a basic culture medium and a second small molecule compound combination, and the culture medium C comprises a basic culture medium and a third small molecule compound combination. The culture medium D comprises a combination of the basic culture medium and a fourth small molecule compound. According to the differential medium composition and the induction method provided by the invention, the mesenchymal stem cells can be prepared from the pluripotent stem cells or the embryonic stem cells.

The application discloses a metformin-induced stem cell-derived extracellular matrix material and a preparation method and application thereof, and relates to the technical field of biological materials. The preparation method comprises the following steps: inoculating mesenchymal stem cells on a pretreated culture carrier, and using a culture system containing metformin to induce and treat the mesenchymal stem cells; adding ascorbic acid to induce and culture extracellular matrix secretion; carrying out decellularization treatment and nuclease treatment on the metformin-induced extracellular matrix; collecting the metformin-induced stem cell-derived extracellular matrix, mixing the metformin-induced stem cell-derived extracellular matrix with an aqueous medium, and enriching and forming an extracellular matrix precipitate through centrifugal treatment; and sequentially carrying out freezing treatment and thawing treatment on the extracellular matrix precipitate to obtain the metformin-induced stem cell-derived extracellular matrix material. The metformin is used to induce the mesenchymal stem cells to secrete an engineered extracellular matrix, and the engineered extracellular matrix is used to promote the repair of bone defects, skeletal muscle injuries and musculoskeletal joint defects in a diabetic environment.

Provided are a preparation method for and a use of a dorsal root ganglion organoid. In one aspect, a preparation method for a dorsal root ganglion organoid is provided, which comprises: (1) inducing stem cells in a neural stem cell induction medium, to obtain neural stem cells; (2) inducing the neural stem cells in a neural crest cell induction medium, to obtain neural crest cells; (3) inducing the neural crest cells in a sensory neural progenitor cell induction medium, to obtain sensory neural progenitor cells; (4) inducing the sensory neural progenitor cells in an organoid induction medium, to obtain a dorsal root ganglion organoid. In addition, a use of the dorsal root ganglion organoid obtained by said method is also provided, for example, for drug screening or disease research.

The present disclosure relates to apoptosis-induced stem cells, a method for producing the same, and a pharmaceutical composition for the prevention or treatment of an inflammatory disease or a renal disease, including the same. Cells according to an aspect and a composition including the cells as an active ingredient have an increased IL-10 expression level without including a cryoprotectant, and thus can be effectively used for the prevention or treatment of an inflammatory disease or a renal disease.

The invention relates to a preparation method of a compound shown as a formula III, which comprises the following step of: demethylating a compound 6 in the presence of a demethylation reagent to prepare the compound shown as the formula III. The compound shown in the formula III is used for stress induction of stem cells to generate functional protein with a repairing effect.

The invention relates to the technical field of biological medicine, in particular to an exosome medicine preparation for treating knee osteoarthritis and a preparation method thereof, and the exosome medicine preparation is characterized in that an exosome is derived from mesenchymal stem cells subjected to three-dimensional culture in a first hydrogel matrix containing sodium alginate and hyaluronic acid; flurbiprofen or a pharmaceutically acceptable salt or ester thereof; and a pharmaceutically acceptable second gel matrix. Sodium alginate and hyaluronic acid composite hydrogel serves as a three-dimensional culture carrier of mesenchymal stem cells, a joint microenvironment is simulated to induce stem cells to secrete high-activity exosomes rich in key functional nucleic acid and active protein, meanwhile, the exosomes are scientifically compounded with flurbiprofen, and synergistic mechanisms of anti-inflammatory analgesia and tissue repair are achieved; and a local slow-release delivery system is constructed through the second gel matrix, so that long-acting stable release of active ingredients is realized, the systemic side effect of the medicine is reduced, the bioavailability of the exosome is improved, and the problems of obvious side effect, limited repair effect and the like in the prior art are solved.

The invention discloses a method for inducing directional differentiation of stem cells by neural factors as well as a preparation and application thereof, and the preparation is a liquid preparation and comprises the following components: BDNF with the concentration of 25-75 ng / mL, GDNF with the concentration of 20-30 ng / mL, NT-3 with the concentration of 5-10 ng / mL and TSA with the concentration of 100-150 nM. The preparation for inducing the directional differentiation of the stem cells through the neural factors can remarkably improve the directional differentiation efficiency of the neural stem cells to neurons, the purity of the neurons is at a high level, the cell viability is good, target cells with mature functions can be efficiently induced, and a new thought is provided for cell treatment of Parkinson's disease.

The application belongs to the technical field of biology, and discloses a kit for inducing stem cells to differentiate into megakaryocytes and / or platelets and application thereof. The kit can induce stem cells to differentiate into megakaryocytes and / or platelets without introducing exogenous cells and exogenous genes. The kit can highly simulate the generation process of megakaryocytes and platelets in vivo, and can induce stem cells into mesendoderm cells, hematopoietic endothelial cells and megakaryocyte-erythroid progenitor cells in sequence. The megakaryocyte-erythroid progenitor cells further develop into megakaryocytes with a multiploid characteristic, and finally produce stem cell-derived platelets with similar functions to natural platelets. The stem cells have the potential for unlimited proliferation, and can be massively expanded in vitro, which provides an unlimited cell source for the in vitro preparation of platelets.

The invention relates to a preparation method of a compound as shown in a formula IV, which comprises the following step of: performing demethylation on a compound 6 in the presence of a demethylation reagent to prepare the compound as shown in the formula IV. The compound as shown in the formula IV is used for stress induction of stem cells to generate functional protein with a repairing effect.

This invention provides a calcium phosphate-polycaprolactone composite scaffold, its preparation method, and its applications. The raw materials for preparing the calcium phosphate-polycaprolactone composite include polycaprolactone, β-tricalcium phosphate, and hydroxyapatite. The resulting composite scaffold possesses functions of promoting immunity and regulating bone regeneration. The preparation method of this invention involves first forming a hydroxyapatite / polycaprolactone composite film, which is then broken down and recombined with β-tricalcium phosphate to form a composite solution. The resulting 3D-printed composite scaffold not only possesses good mechanical strength and biocompatibility but also induces stem cell proliferation and osteogenic differentiation, and induces macrophage regeneration and polarization. It can be used to regulate immune cell responses, induce a bone immune microenvironment conducive to bone formation, thereby enhancing bone regeneration, and can be used for the repair and regeneration of tissue defects.

The invention belongs to the field of biomedical engineering materials, and provides a degradable piezoelectric / conductive cytoskeleton for nerve injury repair, which is prepared according to the following steps: immersing a crosslinked gelatin fiber membrane into excessive graphene oxide liquid, irradiating with ultraviolet light, stirring, and reducing with Na2S2O4. The gelatin fiber membrane with the surface coated with the graphene, which is obtained by the method, can be used for culturing neural stem cells, and the obtained fiber membrane attached with the neural stem cells can be used as a medical engineering material for neural restoration. The graphene and the gelatin fiber are tightly combined to form a continuous phase, so that the conductive performance is excellent, piezoelectric signals generated by the gelatin can be collected and transmitted by the graphene, and more remarkable electric output is generated. The cell scaffold can induce neural differentiation of stem cells in a lead-free mode that electric signals are generated through ultrasonic driving, the risk of infection of external leads is avoided, and the cell scaffold is safer and has remarkable clinical value; and the used materials are gradually degraded in vivo, so that long-term influence in vivo is avoided.

A cell repair-promoting extracellular vesicle, formed by silicate-induced stem cell secretion, helps express anti-inflammatory, tissue regeneration, and cell repair indicator factors, accelerating cell growth in wound tissue and the wound repair process.

The present disclosure relates to apoptosis-induced stem cells, a method for producing the same, and a pharmaceutical composition for the prevention or treatment of an inflammatory disease or a renal disease, including the same. Cells according to an aspect and a composition including the cells as an active ingredient have an increased IL-10 expression level without including a cryoprotectant, and thus can be effectively used for the prevention or treatment of an inflammatory disease or a renal disease.

This invention discloses a method for co-culturing and inducing stem cell differentiation into hematopoietic progenitor cells. The method includes the following steps: culturing pluripotent stem cells to obtain embryoid bodies; culturing the embryoid bodies for mesodermal differentiation to obtain mesodermal cells; culturing the mesodermal cells for hematopoietic endothelial differentiation to obtain hematopoietic endothelial cells; and co-culturing the hematopoietic endothelial cells with mesenchymal stem cells to obtain hematopoietic progenitor cells. Through optimization of the culture system, the differentiation efficiency and the number of hematopoietic progenitor cells obtained are significantly improved; by co-culturing mesenchymal stem cells with hematopoietic endothelial cells, the number of hematopoietic progenitor cells is further increased while maintaining high differentiation efficiency and uniform and stable expression of various markers.

The invention relates to a method for selectively removing undifferentiated pluripotent stem cells, which comprises the following steps of: firstly inducing stem cell differentiation, and then applying an effective amount of phthalide compound to remove the undifferentiated stem cells.

The invention belongs to the technical field of medical stents, and particularly relates to a drug-loaded polydopamine hydrogel filled polycaprolactone stent as well as a preparation method and application thereof. The polycaprolactone scaffold comprises a polycaprolactone scaffold body, and a drug-loading mesoporous ceramic and a zeolite imidazate framework material which are embedded in the polycaprolactone scaffold body, the drug-loaded mesoporous ceramic is embedded in the zeolite imidazate framework material; a base material of the polycaprolactone stent body is polycaprolactone, micron pore structures are arranged between fibers of the polycaprolactone and on the surfaces of the fibers, and the size of the micron pore structures between the fibers is larger than that of the surfaces of the fibers; and the micron pore structure is filled with hydrogel embedded with drug-loaded polydopamine. The polycaprolactone scaffold has good void ratio, biocompatibility and antibacterial property, can induce osteogenic differentiation of stem cells and can accelerate release of antibacterial drugs under the infection condition, and the internally packaged tissue regeneration and repair promoting drugs can be slowly released, so that the polycaprolactone scaffold is suitable for repair and regeneration of infected bone tissue defects.

The present invention provides a method and culture medium for inducing the generation of insulin-secreting cells from stem cells using a specific culture medium and method. This method precisely regulates each stage of differentiation, improving differentiation efficiency and yielding more functional insulin-secreting cells. These cells can be used in diabetes research and treatment, providing a valuable reference and application for subsequent diabetes treatments.

The utility model discloses an electrotransduction instrument for inducing stem cell editing, which comprises an electrotransduction instrument body, an electric shock cup groove is arranged in the electrotransduction instrument body, a groove cover is arranged above the electric shock cup groove, an adjusting screw rod is arranged in the groove cover in a penetrating manner, a connecting bearing is arranged below the adjusting screw rod, and the connecting bearing is connected with the electrotransduction instrument body. A motor is installed above the adjusting lead screw, limiting auxiliary rods are installed on the two sides of the adjusting lead screw, air conveying branch pipes are installed on the two sides of the electric shock cup groove, and high-pressure nozzles are installed on the sides, close to the electric shock cup groove, of the air conveying branch pipes; and the air pump is installed on the right side of the electrotransformer body, and the output end of the air pump is connected with an air delivery pipe. According to the electric conversion instrument for inducing stem cell editing, the air pump blows air into the electric shock cup groove through the air conveying pipe, the air conveying branch pipe and the high-pressure nozzle, dust in the electric shock cup groove is blown out, and the situation that dust left in the electric shock cup groove influences normal operation of the electric conversion instrument is avoided.

The presently disclosed subject matter provides for in vitro methods of inducing differentiation of stem cells into hindbrain progenitor cells, enteric neural crest lineage cells, as well as differentiated hindbrain lineage or enteric neural crest lineage cells obtained by such methods. The presently disclosed subject matter also provides for uses of such cells for preventing and / or treating enteric nervous system disorders (e.g, Hirschsprung's disease), and for screening compounds suitable for preventing and / or treating enteric nervous system disorders (e.g., Hirschsprung's disease).