247 results about "Induced stem cells" patented technology

A method of augmenting and / or repairing an intervertebral disc by administering stem cell material into the disc. The stem cells may be undifferentiated cells, or they may be cells that have differentiated and have subsequently been dedifferentiated. The stem cells may be induced to express at least one characteristic of human intervertebral disc cells, such as fibroblast cells, chondrocyte cells, or notochordal cells, by exposing them to agents and / or environments calculated to induce the desired differentiation. In some embodiments, the stem cell material may be provided in conjunction with a collagen-based material, which may be a collagen-rich lattice or particles of collagen material. The stem cell material may be provided as a stem cell isolate, which may be substantially free of non-stem cell material. Other therapeutic agents may be administered with the stem cell material.

For a method of inducing differentiation of cardiomyocytes from stem cells, a method is provided to induce efficiently and selectively differentiation of cardiomyocytes by such a method in which the stem cells are cultured to induce differentiation into cardiomyocytes in the presence of a substance that inhibits BMP signaling.

The invention discloses a double-layer membranous tissue repair material and a preparation method thereof, wherein, a cell-free membranous biological derivative material is used as a surface layer, and a fibroblast is compounded in the interior of a biological support material to form a substrate, and then the surface layer and the substrate are combined in a chimeric way to form the double-layer membranous tissue repair material; a compact surface layer structure can effectively reduce the loss of water, electrolytes and protein from surface of wound, avoid the invading and the reproduction of bacteria to the impaired surface of wound as well as prevent the infection of the surface of wound, thus being beneficial to epitheliosis and epithelial growth; the substrate can directly repair the surface of wound, promote the ingrowth of cells around the surface of wound and the angiogenesis, induce the differentiation from stem cells to skin cells and quicken wound healing; compared with the existing products, the tissue repair material has the advantages of being capable of promoting the regeneration of skin, improving the elasticity, the flexibility and the mechanical abrasion resistance of skin after the surface of wound is healed, reducing hyperplasia of scar tissues, controlling the contracture, having excellent biocompatibility, increasing the success rate of transplant and improving the quality of healing; the invention has wide material resources and simple production method; the double-layer membranous tissue repair material prepared is applicable to the clinical treatment of skin defect caused by inflammation, ulcer, thermal burns, iatrogenicity and the like.

The present invention relates to methods of inducing differentiation of stem cells. In particular, the invention relates to methods of inducing differentiation of embryonic stem cells into muscle cells or vascular endothelial cells. The invention also includes cells, cell lines, testing models and culture systems used in the methods of the present invention and differentiated cells produced therefrom. The present invention also provides methods of using the differentiated cells of the present invention for therapeutic purposes.

The invention relates to a serum-free culture medium capable of fast and effectively inducing somatic cells to be reprogrammed to produce pluripotent stem cells and a method for inducing stem cells to be reprogrammed by using the serum-free culture medium without feeder, wherein the speed and efficiency of induced reprogramming are greatly increased. Moreover, the invention further relates to the use of the culture medium for inducing pluripotent stem cells and a method for screening of compounds, in particular for the high-throughput screening of compounds.

The invention provides icariin application in inducing stem cell in vitro orientation differentiates to several single type cells. The stem cell said includes embryonic stem cell, nerve stem cell and marrow mesenchyme stem cell. The single type cell includes nerve cell, bone cell, islet cells and endothelial cell. This invention also relates to application of the single type cell in preparing medicine of stem cell transplant curing nerve degenerative diseases, and its application in preparing cell differentiation agent that used to repair recovery injured nerve tissue, and its application in high efficiency drug effect screening model rebuilding and initial screening and estimating by using the model. The new applications of the icariin is extended in this invention, the fact of icariin contains pharmacy activity clarified, so it provides material basis for Chinese traditional medicine prevention and cure effect. The clarifying of drug effect mechanism provides reference for new medicine Chinese metical modern development with self-owned intellectual property right.

The invention discloses a double-layer artificial skin and a preparation method thereof, wherein, cell-free membranous biological derivative material is used as a surface layer, and a fibroblast, extracellular matrix synthesized and secreted by the fibroblast and a cell growth factor are compounded in the interior of biological support material to form a dermis, and then the surface layer and the dermis are combined to form the double-layer artificial skin; a compact surface layer structure can effectively reduce the loss of water, electrolytes and protein from surface of wound, avoid the invading and the reproduction of bacteria to the impaired surface of wound as well as the infection of the surface of wound, and be beneficial to epitheliosis and epithelial growth; the dermis can directly repair the surface of wound, promote the ingrowth of cells around the surface of wound and the angiogenesis, induce the differentiation from stem cells to skin cells and quicken wound healing; the artificial skin has the advantages of being capable of promoting the regeneration of skin, improving the elasticity, the flexibility and the mechanical abrasion resistance of skin after the surface of wound is healed, reducing excess scar tissues, controlling the contracture, having excellent biocompatibility, increasing the success rate of transplant and improving the quality of healing; the double-layer artificial skin has wide material resources and simple production method, and is applicable to the clinical treatment of skin defect caused by inflammation, ulcer, thermal burns, iatrogenicity and the like.

The invention relates to a humanized heterogenous extracellular matrix material, which is a dried cellular foam material made by compounding an extracellular matrix and a cell growth factor which are synthesized and excreted by human body cells on a heterogenous extracellular matrix. Cells and natural antigen components are removed from the prepared humanized heterogenous extracellular matrix material, and when the humanized heterogenous extracellular matrix material is applied to the surface of wound, the humanized heterogenous extracellular matrix material can directly take part in the repair of the surface of wound, and the cell growth factor contained in the humanized heterogenous extracellular matrix material can guide the ingrowth of the cells around the wound and creation of blood vessel, and induce the differentiation of stem cells to skin cells, thereby obviously promoting the healing of the surface of wound; besides, the prepared product is a dried product, which greatly prolongs storage life, and not only has part of the characteristics of human body tissues and good mechanical property of the heterogenous extracellular matrix, but also has higher biocompatibility to human body and obviously reduces the immune exclusive reaction; at the same time, the prepared product can cover the surface of wound, fill the defection of soft tissues, promote the growth and proliferation of the cells around the wound, repair the defection of the soft tissue organs, and promote the wound healing.

The potential of a stem cell to differentiate into specialized cell types for restoring normal tissue / organ function has stimulated interest in stem cell research. The methods used to coax stem cells differentiate into specialized cells still remain in their infancy stages. The disclosed invention is the generation of mammalian or avian cell hybrids formed from fusing lineage committed somatic cells with nucleated stem cells or nucleated transit amplifying cells. The fusion of lineage committed somatic cells with nucleated stem cells, or nucleated transit amplifying cells as described herein facilitates stem cell differentiation and lineage commitment of hybrid cells and can be aided by inclusion of an encapsulation step. By the fusion of cells in this invention, this invention also provides for methods to restore damaged tissue or the expression of defective, dysfunctional, decreased, lost or not previously expressed bio-pharmaceutical products.

The invention provides a cell preparation for treating osteoarthritis and a preparation method thereof. The cell preparation mainly comprises mesenchymal stem cells, differentiation-induced stem cells, platelet-rich plasma and dispersion media thereof. Compared with the prior art, the cell preparation for treating osteoarthritis has the advantages that the mesenchymal stem cells are mixed with the differentiation-induced stem cells and compounded with the platelet-rich plasma and can be dispersed in the joint cavity, so that the immune regulation function of the mesenchymal stem cells can be exerted, the microenvironment of the whole joint cavity can be improved, and inflammation remission and injury repair are facilitated; tissues can be repaired after the differentiated mesenchymal stem cells are implanted into the body, so that the repair process is shortened.

Methods of generating, and isolating adult stem cells and utilizing such cells and / or embryonic stem cells in generating tissue of a specific function and micro-architecture are provided.

Dynamic vibrational methods and devices for inducing differentiation of stem cells into vocal fold fibroblast-like cells or for generating vocal fold-like tissue from cultured cells. Also provided are matrices providing sustained release of growth factors, and bioreactors generating and delivering a high frequency vibration with in-plane shear stress to cultured cells.

The present invention relates to screens for compounds that can induce stem cell differentiation. In addition, isoxazoles and sulfonyl hydrazones are identified as general classes of compounds that can induce differentiation of stem cells into cells of neuronal and cardiac fate, respectively.

The invention discloses a composite material of organic / inorganic multi-phase induction nano-hydroxyapatite. The composite material is characterized in that on the basis of the effect that graphene oxide can enhance adhesion of stem cells, and stem cells are induced to be differentiated into bone cells and adsorb organic and inorganic nanoparticles, chitosan and bovine collagen are adopted as organic matrixes, a graphene oxide water solution is adopted as an inorganic matrix, a soluble calcium salt and a soluble phosphate are adopted as a precursor of inorganic phase nano-hydroxyapatite, a biological mechanism and an in-situ composite preparation technology are adopted, and the composite material of organic / inorganic multi-phase induction nano-hydroxyapatite is prepared bionically. The preparation conditions are mild, the composite material is uniform in pore diameter, the hole forming performance is good, the biocompatibility and biodegradability are good, and the composite material is expected to be the novel composite material for treating osteoporosis.

The invention discloses a method for preparing retinal pigment epithelia. According to the method, stem cells are induced by utilizing a liver X receptor (LXR) activating agent to be differentiated into the retinal pigment epithelia. Compared with the prior art, the method for inducing the stem cells to be differentiated into the retinal pigment epithelia by utilizing the LXR receptor activating agent has the advantages that: a feed layer is not used, so that potential risks caused by an animal source feed layer are avoided; and the differentiation efficiency can be improved, and the differentiation time can be shortened.

The invention provides a method of efficiently producing corneal endothelial cells, particularly from corneal stroma or iPS cell-derived neural crest stem cells, a method of producing corneal endothelial cells stably in a large amount by inducing more efficient differentiation of stem cells into corneal endothelial cells, and a medicament containing corneal endothelial cells. The method of inducing differentiation of stem cells into corneal endothelial cells includes a step of culturing the stem cells in a differentiation induction medium containing a GSK3 inhibitor (preferably a GSK3β inhibitor) and retinoic acid, with the differentiation induction medium preferably further containing one or more of TGFb2, insulin, a ROCK inhibitor, and the like.

The present invention relates to methods, kits, combinations, and compositions for treating, preventing or reducing the risk of developing a disorder or disease related to, or the symptoms associated with a neurodegenerative disorder such as neurotoxicity or a neuropathology in a subject, particularly to beta-amyloid-induced neurotoxicity and Alzheimer's disease. The invention further provides a method for inducing stem cell differentiation into neuronal cells, by administering to the patient a therapeutically effective amount of a compound of the invention.

The present invention relates to the induction of differentiation in stem cells to cardiomyocytes and factors such as prostaglandin alone or in combination with other factors including essential minerals selected from the group including transferrin and selenium, small molecules selected from the group including a p38 MAPK inhibitor such as SB203580 and protein growth factors of the FGF, IGF and BMP families such as but not limited to IGF1, FGF2, BMP2, BMP4 and BMP6. and insulin that influence the process of differentiation to cardiomyocytes. Media that is appropriate for the induction of differentiation of cardiomyocytes from stem cells is also provided wherein the media contains these factors. The use of cardiomyocytes and cardiac progenitors produced by the directed differentiation in transplantation and screening for cardiac compounds is also provided.

A method for preparing chondroblast of bone marrow mesogalia stem cell with three dimensional cultivation and induction includes steps of placing bone marrow mesogalia stem cell into suspending culture device preset with culture medium and microcarrier for suspending cultivation; cloning abovesaid stem cells with agitation, adding induction culture medium of chondroblast in culture meidum containing harvested cytodex3 microcrrier and abovesaid stem cells gromn on it; and carrying out induction for chondroblast assembly from abovesaid stem cells.

Provided is a differentiation-inducing culture medium additive for inducing bone differentiation of at least one type of cell selected from the group consisting of a stem cell, a dental pulp cell, a periodontal ligament cell, a placenta, an amnion, and a fibroblast under a serum-free condition, and a use of the differentiation-inducing culture medium additive. The differentiation-inducing culture medium additive of the present invention for inducing differentiation of a stem cell under a serum-free condition at least contains at least one growth factor selected from the group consisting of EGF, FGF, and PDGF; dexamethasone; and β-glycerophosphate. The differentiation-inducing culture medium additive of the present invention does not require ascorbic acid 2-phosphate and ITS, which are normally essential for bone differentiation. Further, bone differentiation can be promoted by adding phospholipid.

A composite comprising a stem cell; a biodegradable layer, which can provide an environment for the stem cell to grow and to differentiate, and; a N-isopropylacrylamide (NIPAAm), which can polymerize with the biodegradable layer and possess the temperature-responsive character for easy stripping. This invention also provides a method of treating a subject with a skin defect by covering the composite of the present invention on the skin defect of the subject in need of such treatment. Furthermore, using this composite with different growth factors, stem cells can be induced to differentiate into skin-related, neuronal cells, neuron, and insulin-positive cells in biodegradable scaffolds as well as transplanted graft. Finally, this invention also provides a quick and convenient method of monitoring cell growth or tissue engineering in an animal.

For a method of inducing differentiation of cardiomyocytes from stem cells, a method is provided to induce efficiently and selectively differentiation of cardiomyocytes by such a method in which the stem cells are cultured to induce differentiation into cardiomyocytes in the presence of a substance that inhibits BMP signaling.

A method for inducing the differentiation of stem cells into tissue cells; a bioartificial organ, which contains the tissue cells; and a material for medical purposes, which contains the bioartificial organ are disclosed. Stem cells capable of proliferation, self-replication and differentiation are used and cultured by a hanging-drop method to three-dimensionally construct a cell mass of embryoid bodies, and the cell mass is cultured in the presence of HGF, GDNF, b-FGF, BMP7 and EGF. In this manner, the stem cells can be differentiated into tissue cells. A bioartificial organ can be produced using cells that have been differentiated into the tissue cells. Further, a material for medical purposes can be provided.

A method for differentiating or promoting or inducing differentiation of stem cells into pluripotent cardiovascular progenitors (MCPs) by transiently inducing the expression of a single gene, namely Mesp1, is disclosed. Cells obtained by the method and their uses in research and clinical settings are also disclosed. Using genome wide transcriptional analysis, the inventors found that Mesp1 rapidly activates and represses a discrete set of genes, which form potential new targets for both therapy and for the identification of MCPs. Insights into the molecular mechanisms underlying the earliest step of cardiovascular specification and potential methods for dramatically increasing the number of cardiovascular cells for cellular therapy in humans are provided.

The present invention relates to screens for compounds that can induce stem cell differentiation. In addition, isoxazoles and sulfonyl hydrazones are identified as general classes of compounds that can induce differentiation of stem cells into cells of neuronal and cardiac fate, respectively.

The invention relates to the field of stem cell and tissue engineering, and discloses a method for inducing in vitro directed differentiation of stem cells through non-contact coculture. The method comprises the following steps of: embedding inducing cells with function of inducing differentiation in biological microcapsules; and coculturing the microcapsules and the stem cells, so that the stem cells are subjected to directed induction and differentiation through the directed induction function of the inducing cells in the microcapsules. The method is easy to operate; and one or more kinds of dioecious and heterogeneous inducing cells are allowed to be used for promoting the in vitro directed differentiation of the stem cells, namely microcapsule films can make inducing factors secreted by the inducing cells permeate the microcapsules to act on the external stem cells, and the inducing cells and the stem cells can be subjected to imunoisolation and do not contact each other directly to facilitate the separation and harvest of different cells. By the method, in vitro directed induction and differentiation of the stem cells can be realized under the condition of conventional static culture, an effective induction method can be provided for dynamically inducing directed differentiation of the stem cells to form three-dimensional tissues in a biological reactor, and the method has important significance for clinical therapeutic research of damaged tissues.

The invention relates to a tissue repairing material with bioactivity and a preparation method thereof. The material is made by compounding human body living cells, and extracellular matrixes and cell growth factors synthesized and excreted by the human body living cells on the acellular small intestine submucosa. Natural antigen components are removed from the prepared tissue repairing material with bioactivity, and when the tissue repairing material is applied to a wound, the tissue repairing material can survive and directly take part in the repair of the wound to continuously synthesize and excrete growth factors, guide the cells around the wound to grow in and the creation of blood vessel, and induce the differentiation of stem cells to skin cells, thereby obviously promoting the wound healing; besides, the tissue repairing material not only has part of the characteristics of human body tissues and the good mechanical property of the acellular small intestine submucosa, but also has high biocompatibility to human body, obviously reduces the immune rejection; at the same time, the prepared tissue repairing material can cover the surface of wound, fill the defection of soft tissues, promote the growth and proliferation of the cells around the wound, repair the defection of the soft tissue organs, and promote the wound healing.

The invention provides a method of efficiently producing corneal endothelial cells, particularly from corneal stroma or iPS cell-derived neural crest stem cells, a method of producing corneal endothelial cells stably in a large amount by inducing more efficient differentiation of stem cells into corneal endothelial cells, and a medicament containing corneal endothelial cells. The method of inducing differentiation of stem cells into corneal endothelial cells includes a step of culturing the stem cells in a differentiation induction medium containing a GSK3 inhibitor (preferably a GSK3β inhibitor) and retinoic acid, with the differentiation induction medium preferably further containing one or more of TGFb2, insulin, a ROCK inhibitor, and the like.

Disclosed is a composition for inducing the proliferation of retinal cells or the differentiation of retinal progenitor cells into retinal cells. The composition, similar to in vivo conditions for development during embryogenesis, induces stem cells to differentiate into a multitude of photoreceptor cells at high yield within a short period of time, without an additional gene transfer. In addition, the differentiated photoreceptor cells are useful in cellular therapy because they, when transplanted into degenerated or injured retinas, can be engrafted and fused within the retinas to prevent or cure retinal degeneration.

The invention provides a pancreatic-like structure and a construction method thereof. The construction method comprises the following steps: A, inducing stem cells and / or progenitor cells in vitro todifferentiate into pancreatic-like cell clusters; B, mixing the vascularized cells, the pancreatic-like cell clusters and a hydrogel material, and performing biological printing to obtain a pre-gel three-dimensional structure body; and C, culturing the pre-gel three-dimensional structure body by adopting a multi-cell culture solution and / or a bioreactor to obtain the pancreas-like structure body.The pancreatic-like structure provided by the invention is composed of vascularized cells and pancreatic-like tissue cells, and has the form, phenotypic characteristics and physiological functions ofnatural tissues. The method can be applied to the aspects of organoid construction, tissue / organ / human body chips, tissue engineering, regenerative medicine, in-vitro physiological model / pathologicalmodel / pharmacological model construction, cytobiology or drug research and the like.