72 results about "Induced stem cells" patented technology

The invention discloses preparation and application of dual-network hydrogel. The dual-network hydrogel comprises the following components: (Z1) a spinal cord acellular matrix network DSM formed by self-assembly of a spinal cord acellular matrix dSECM; and (Z2) a metal-polyphenol network MPN formed by coordination of metal ions and polyphenols; wherein the DSM and the MPN are cross-linked through hydrogen bonds to jointly form a double-network structure. DSM provides a microenvironment suitable for cell survival and growth for the dual-network hydrogel and induces stem cells to differentiate into neurons, and MPN provides the dual-network hydrogel with biological effects of inhibiting inflammation and accumulation of reactive oxygen species (ROS). The dual-network hydrogel has the advantages of the two components, the active component neurotrophic factor and the inhibitor loaded on the dual-network hydrogel can regulate and control immune response after SCI, improve neuroinflammation microenvironment and promote nerve repair, and a new strategy is provided for in-situ tissue repair of SCI.

The invention provides a preparation method of gas-jet mineralized collagen-hydrogel microspheres, and relates to the technical field of biological materials, and the preparation method comprises the following steps: dissolving collagen in a phosphate solution, dropwise adding the phosphate solution and a CaCl2 solution into a continuously stirred alkaline solution together, reacting for 12-48 hours, centrifugally collecting a precipitate, washing with water, centrifuging, and freeze-drying to obtain mineralized collagen; the method comprises the following steps: dissolving mineralized collagen and sodium alginate in deionized water, spraying the solution into a CaCl2 solution through a gas spraying spinning head by adopting a gas spraying technology, collecting microspheres, and repeatedly cleaning by adopting deionized water to obtain the gas spraying mineralized collagen-hydrogel microspheres. Mineralized collagen is encapsulated and loaded through microspheres, so that interferences of tissue fluid are isolated, and non-specific aggregation is reduced; optimizing the ratio of mineralized collagen, and directionally inducing stem cells to differentiate; the diameter of the microsphere is accurately controlled by regulating and controlling gas injection parameters to adapt to different wounds; active ingredients are controlled according to needs through a microsphere cross-linked network, and the multi-stage requirements of subcutaneous repair are met.

The invention discloses a cerium nano-enzyme composite active material and application thereof in hepatic failure treatment, and belongs to the technical field of nano-materials. The invention combines the functions of miR122 and cerium dioxide nano-enzyme, develops a new stem cell differentiation inducer, namely a cerium nano-enzyme composite active material, and deeply studies the potential mechanism of the nano-enzyme material with catalytic activity in regulating stem cell fate, and the cerium dioxide nano-enzyme can be used for delivering miR122 into stem cells after being modified, so that the stem cell differentiation inducer can be used for inducing stem cell differentiation. The miR122 is highly enriched in cells, so that hepatic differentiation is induced. Meanwhile, the catalytic activity of the cerium dioxide nano-enzyme can regulate the ROS steady state in the cells and protect mitochondria from being damaged by ROS induction, so that the mitochondrial function is maintained, the energy requirement in the differentiation process is met, and the method is of great importance to successful differentiation of the stem cells. In order to solve the problem of activity damage in hepatic cell transplantation, a layer of Ce < 4 + >-polyphenol skeleton is coated on the surface of induced hepatic-like cells (iHLCs), so that the function and activity of the hepatic cells after transplantation are further protected, and the in-vivo treatment effect of the hepatic cells is improved.

Provided are a reagent combination or a kit for constructing an embryoid and the use thereof. The reagent combination or the kit can be used for inducing a stem cell to produce a blastocyst-like lineage precursor cell, a blastocyst-like cell and / or an embryoid, which are based on small-molecule induction and independent of transgenes, and have a single cell source. The produced blastocyst-like lineage precursor cell and blastocyst-like cell have a full blastocyst lineage, and are seed cells having balanced developmental competence and totipotency, which solves the problem of unbalanced cell maturation in the prior art. The produced embryoid is highly similar to natural embryos in terms of morphological characteristics and transcriptomic characteristics, has the capability of highly reproducing post-implantation embryonic development and / or gastrulation, which is beneficial for research on embryonic development in vitro. The method for inducing the stem cell to produce the blastocyst-like lineage precursor cell, the blastocyst-like cell and / or the embryoid by using the reagent combination or the kit is simple, and has high applicability and high embryoid construction efficiency.

Described herein are methods of inducing proliferation of PRX1 expressing stem cells stem cells in a subject in need thereof, the method can include administering an effective amount of niclosamide, or a pharmaceutically acceptable salt, prodrug, or derivative thereof.

The invention provides a differential medium composition and a method for preparing mesenchymal stem cells by inducing stem cells to differentiate in a body segment direction, and belongs to the field of biology. The differential culture medium combination comprises a culture medium A, a culture medium B, a culture medium C and a culture medium D. The culture medium A comprises a basic culture medium and a first small molecule compound combination, the culture medium B comprises a basic culture medium and a second small molecule compound combination, and the culture medium C comprises a basic culture medium and a third small molecule compound combination. The culture medium D comprises a combination of the basic culture medium and a fourth small molecule compound. According to the differential medium composition and the induction method provided by the invention, the mesenchymal stem cells can be prepared from the pluripotent stem cells or the embryonic stem cells.

The application discloses a metformin-induced stem cell-derived extracellular matrix material and a preparation method and application thereof, and relates to the technical field of biological materials. The preparation method comprises the following steps: inoculating mesenchymal stem cells on a pretreated culture carrier, and using a culture system containing metformin to induce and treat the mesenchymal stem cells; adding ascorbic acid to induce and culture extracellular matrix secretion; carrying out decellularization treatment and nuclease treatment on the metformin-induced extracellular matrix; collecting the metformin-induced stem cell-derived extracellular matrix, mixing the metformin-induced stem cell-derived extracellular matrix with an aqueous medium, and enriching and forming an extracellular matrix precipitate through centrifugal treatment; and sequentially carrying out freezing treatment and thawing treatment on the extracellular matrix precipitate to obtain the metformin-induced stem cell-derived extracellular matrix material. The metformin is used to induce the mesenchymal stem cells to secrete an engineered extracellular matrix, and the engineered extracellular matrix is used to promote the repair of bone defects, skeletal muscle injuries and musculoskeletal joint defects in a diabetic environment.

Provided are a preparation method for and a use of a dorsal root ganglion organoid. In one aspect, a preparation method for a dorsal root ganglion organoid is provided, which comprises: (1) inducing stem cells in a neural stem cell induction medium, to obtain neural stem cells; (2) inducing the neural stem cells in a neural crest cell induction medium, to obtain neural crest cells; (3) inducing the neural crest cells in a sensory neural progenitor cell induction medium, to obtain sensory neural progenitor cells; (4) inducing the sensory neural progenitor cells in an organoid induction medium, to obtain a dorsal root ganglion organoid. In addition, a use of the dorsal root ganglion organoid obtained by said method is also provided, for example, for drug screening or disease research.

The present disclosure relates to apoptosis-induced stem cells, a method for producing the same, and a pharmaceutical composition for the prevention or treatment of an inflammatory disease or a renal disease, including the same. Cells according to an aspect and a composition including the cells as an active ingredient have an increased IL-10 expression level without including a cryoprotectant, and thus can be effectively used for the prevention or treatment of an inflammatory disease or a renal disease.

The invention relates to the technical field of biological medicine, in particular to an exosome medicine preparation for treating knee osteoarthritis and a preparation method thereof, and the exosome medicine preparation is characterized in that an exosome is derived from mesenchymal stem cells subjected to three-dimensional culture in a first hydrogel matrix containing sodium alginate and hyaluronic acid; flurbiprofen or a pharmaceutically acceptable salt or ester thereof; and a pharmaceutically acceptable second gel matrix. Sodium alginate and hyaluronic acid composite hydrogel serves as a three-dimensional culture carrier of mesenchymal stem cells, a joint microenvironment is simulated to induce stem cells to secrete high-activity exosomes rich in key functional nucleic acid and active protein, meanwhile, the exosomes are scientifically compounded with flurbiprofen, and synergistic mechanisms of anti-inflammatory analgesia and tissue repair are achieved; and a local slow-release delivery system is constructed through the second gel matrix, so that long-acting stable release of active ingredients is realized, the systemic side effect of the medicine is reduced, the bioavailability of the exosome is improved, and the problems of obvious side effect, limited repair effect and the like in the prior art are solved.

The invention discloses a method for inducing directional differentiation of stem cells by neural factors as well as a preparation and application thereof, and the preparation is a liquid preparation and comprises the following components: BDNF with the concentration of 25-75 ng / mL, GDNF with the concentration of 20-30 ng / mL, NT-3 with the concentration of 5-10 ng / mL and TSA with the concentration of 100-150 nM. The preparation for inducing the directional differentiation of the stem cells through the neural factors can remarkably improve the directional differentiation efficiency of the neural stem cells to neurons, the purity of the neurons is at a high level, the cell viability is good, target cells with mature functions can be efficiently induced, and a new thought is provided for cell treatment of Parkinson's disease.

The application belongs to the technical field of biology, and discloses a kit for inducing stem cells to differentiate into megakaryocytes and / or platelets and application thereof. The kit can induce stem cells to differentiate into megakaryocytes and / or platelets without introducing exogenous cells and exogenous genes. The kit can highly simulate the generation process of megakaryocytes and platelets in vivo, and can induce stem cells into mesendoderm cells, hematopoietic endothelial cells and megakaryocyte-erythroid progenitor cells in sequence. The megakaryocyte-erythroid progenitor cells further develop into megakaryocytes with a multiploid characteristic, and finally produce stem cell-derived platelets with similar functions to natural platelets. The stem cells have the potential for unlimited proliferation, and can be massively expanded in vitro, which provides an unlimited cell source for the in vitro preparation of platelets.

This invention provides a calcium phosphate-polycaprolactone composite scaffold, its preparation method, and its applications. The raw materials for preparing the calcium phosphate-polycaprolactone composite include polycaprolactone, β-tricalcium phosphate, and hydroxyapatite. The resulting composite scaffold possesses functions of promoting immunity and regulating bone regeneration. The preparation method of this invention involves first forming a hydroxyapatite / polycaprolactone composite film, which is then broken down and recombined with β-tricalcium phosphate to form a composite solution. The resulting 3D-printed composite scaffold not only possesses good mechanical strength and biocompatibility but also induces stem cell proliferation and osteogenic differentiation, and induces macrophage regeneration and polarization. It can be used to regulate immune cell responses, induce a bone immune microenvironment conducive to bone formation, thereby enhancing bone regeneration, and can be used for the repair and regeneration of tissue defects.

The present disclosure relates to apoptosis-induced stem cells, a method for producing the same, and a pharmaceutical composition for the prevention or treatment of an inflammatory disease or a renal disease, including the same. Cells according to an aspect and a composition including the cells as an active ingredient have an increased IL-10 expression level without including a cryoprotectant, and thus can be effectively used for the prevention or treatment of an inflammatory disease or a renal disease.

This invention discloses a method for co-culturing and inducing stem cell differentiation into hematopoietic progenitor cells. The method includes the following steps: culturing pluripotent stem cells to obtain embryoid bodies; culturing the embryoid bodies for mesodermal differentiation to obtain mesodermal cells; culturing the mesodermal cells for hematopoietic endothelial differentiation to obtain hematopoietic endothelial cells; and co-culturing the hematopoietic endothelial cells with mesenchymal stem cells to obtain hematopoietic progenitor cells. Through optimization of the culture system, the differentiation efficiency and the number of hematopoietic progenitor cells obtained are significantly improved; by co-culturing mesenchymal stem cells with hematopoietic endothelial cells, the number of hematopoietic progenitor cells is further increased while maintaining high differentiation efficiency and uniform and stable expression of various markers.

The utility model discloses an electrotransduction instrument for inducing stem cell editing, which comprises an electrotransduction instrument body, an electric shock cup groove is arranged in the electrotransduction instrument body, a groove cover is arranged above the electric shock cup groove, an adjusting screw rod is arranged in the groove cover in a penetrating manner, a connecting bearing is arranged below the adjusting screw rod, and the connecting bearing is connected with the electrotransduction instrument body. A motor is installed above the adjusting lead screw, limiting auxiliary rods are installed on the two sides of the adjusting lead screw, air conveying branch pipes are installed on the two sides of the electric shock cup groove, and high-pressure nozzles are installed on the sides, close to the electric shock cup groove, of the air conveying branch pipes; and the air pump is installed on the right side of the electrotransformer body, and the output end of the air pump is connected with an air delivery pipe. According to the electric conversion instrument for inducing stem cell editing, the air pump blows air into the electric shock cup groove through the air conveying pipe, the air conveying branch pipe and the high-pressure nozzle, dust in the electric shock cup groove is blown out, and the situation that dust left in the electric shock cup groove influences normal operation of the electric conversion instrument is avoided.

The presently disclosed subject matter provides for in vitro methods of inducing differentiation of stem cells into hindbrain progenitor cells, enteric neural crest lineage cells, as well as differentiated hindbrain lineage or enteric neural crest lineage cells obtained by such methods. The presently disclosed subject matter also provides for uses of such cells for preventing and / or treating enteric nervous system disorders (e.g, Hirschsprung's disease), and for screening compounds suitable for preventing and / or treating enteric nervous system disorders (e.g., Hirschsprung's disease).

The invention belongs to the technical field of medical dressings, and relates to a DNA hydrogel dressing with a skin-like structure and function as well as a preparation method and application of the DNA hydrogel dressing. The dressing is structurally characterized in that a skin-like three-layer structure, namely an epidermal layer, a corium layer and a subcutaneous tissue layer, is constructed by regulating and controlling the cross-linking degree of chain segments and combining a directional freezing technology, and an extracellular matrix microenvironment is provided for physiological activities of cells. In function, an aptamer sequence with a specific function is introduced, and stem cells are induced to directionally differentiate into cells required by tissue repair; the subcutaneous tissue-like layer is loaded with resveratrol, and fascia is activated to induce regeneration of skin appendages; in view of the fact that chronic ulcerative wounds are easily attacked by bacteria, the antibacterial peptide and a functional DNA sequence of targeted bacteria form an antibacterial peptide-DNA composite chain segment through a click reaction, and the dressing is endowed with targeted antibacterial performance. The prepared dressing has a skin-imitating three-dimensional structure and performance, can guide skin appendages such as regeneration of hair follicles and sweat glands, and can be widely applied to repair treatment of chronic wounds.

The present application provides the use of leukotrienes in inducing stem cells to differentiate into hematopoietic stem / progenitor cells and the application thereof. According to specific embodiments of the present application, the addition of appropriate amounts of leukotrienes during the process of inducing stem cells to differentiate into hematopoietic stem / progenitor cells can significantly improve the differentiation efficiency of the stem cells into hematopoietic stem / progenitor cells, and significantly increase the number of erythroid cells, megakaryocytic cells, granulocytic cells and mononuclear cells obtained after the differentiation of the stem cells through hematopoietic stem / progenitor cells.

The invention belongs to the technical field of biology, and discloses a reagent combination or kit for constructing an embryoid and application of the reagent combination or kit. The reagent combination or the kit can be used for inducing stem cells to generate blastocyst-like pedigree precursor cells, blastocyst-like cells and / or embryoid-like cells, is based on small molecule induction, does not depend on transgenosis, and is single in cell source, and the generated blastocyst-like pedigree precursor cells and blastocyst-like cells have a whole blastocyst pedigree, and can be used for inducing stem cells to generate blastocyst-like pedigree precursor cells and blastocyst-like cells. The seed cells are balanced in developmental ability and have totipotency, and the problem of unbalanced cell maturation generated in the prior art is solved; the generated embryoid is highly similar to a natural embryo in morphological characteristics and transcriptome characteristics, has the capability of highly reproducing embryonic development and / or protointestinal movement after implantation, and is beneficial to in-vitro research of embryonic development; the reagent combination or the kit is used for inducing stem cells to generate blastocyst-like pedigree precursor cells, blastocyst-like cells and / or embryoid-like cells, and the method is simple, high in applicability and high in embryoid-like construction efficiency.

The invention provides a differential medium composition and a method for preparing mesenchymal stem cells by inducing stem cells to differentiate in a bone joint direction. The differential culture medium combination comprises a culture medium A, a culture medium B, a culture medium C, a culture medium D and a differential culture medium E. The culture medium A comprises a basic culture medium and a first small molecule compound combination, and the culture medium B comprises a basic culture medium and a second small molecule compound combination. The culture medium C comprises the basic culture medium and a third small molecule compound combination, the culture medium D comprises the basic culture medium and a fourth small molecule compound combination, and the differential culture medium E comprises the basic culture medium and a fifth small molecule compound combination. According to the differential medium composition and the induction method, the mesenchymal stem cells can be prepared by inducing the pluripotent stem cells to differentiate in the direction of the bone segments.

The present application relates to methods for inducing the differentiation of stem cells into chondrocyte progenitors and chondrocytes in vitro. The methods also relate to the production of in vitro-engineered cartilage and bone and related biomaterials as well as methods of drug-screening and modeling the bone- and cartilage-related diseases and disorders.

The invention discloses application of H3K9me2 as a target spot in preparation of a preparation for inducing stem cells to differentiate to gametes in vitro, and relates to the technical field of stem cell breeding in animal husbandry. An H3K9me2 inhibitor BIX01294 is adopted as a treating agent, and in-vivo treatment is performed on a pregnant mouse and in-vitro treatment is performed on gonad tissue of a female fetal mouse. The result shows that the BIX01294 treatment can cause the reduction of the expression level of H3K9me2, so that the expression levels of pluripotent genes Oct4, Sox2 and Dppa3 are increased, the accessibility of whole genome chromatin is obviously changed, and the meiosis start and meiosis process are blocked. The result shows that the H3K9me2 can influence the differentiation of the stem cells to the gametes and is beneficial to the generation of artificial gametes in the stem cell breeding process.

The present disclosure relates to a method for induced differentiation of stem cells into hematopoietic progenitor cells. The method comprises the following steps: culturing pluripotent stem cells to obtain an embryoid body; performing mesoderm differentiation culture on the embryoid body to obtain mesoderm cells; performing hemogenic endothelium differentiation culture on the mesoderm cells to obtain hemogenic endothelial cells; and performing hematopoietic progenitor cell differentiation culture on the hemogenic endothelial cells to obtain hematopoietic progenitor cells. According to the present disclosure, the hematopoietic progenitor cells can be rapidly and efficiently prepared, and the prepared hematopoietic progenitor cells have an ability to stably differentiate into a plurality of different blood cells (including simultaneously having erythroid, myeloid, and lymphoid cells); by optimizing a culture system, the differentiation efficiency is significantly improved and the number of hematopoietic progenitor cells obtained is significantly increased.

This invention provides a stem cell preparation for skin repair and its application, belonging to the field of biomedical technology. The stem cell preparation is obtained by inducing stem cells in a composite culture medium containing sodium butyrate, L-carnosine, and quercetin, followed by short-term pulsed hypoxia treatment, ultrasonic disruption, deep freeze-thaw rehydration, and vacuum freeze-drying to obtain a lyophilized stem cell powder. Experimental results show that the stem cell preparation of this invention significantly promotes the proliferation of human skin fibroblasts and significantly promotes the migration of human keratinocytes. In a mouse full-thickness skin defect model, local application after reconstitution in PBS buffer containing 0.1% sodium hyaluronate (concentration ≥100 μg / mL) significantly accelerates wound closure. In summary, the preparation of this invention exhibits high activity, strong stability, and broad prospects for clinical translation.

Provided are a culture medium composition for inducing differentiation from stem cells to platelet precursor cells and a method of inducing differentiation from stem cells to platelet precursor cells in the presence of said culture medium composition. According to one aspect, the culture medium composition for inducing differentiation from stem cells to platelet precursor cells can enhance the efficiency of differentiation into platelet precursor cells and has the effect of differentiating into platelet precursor cells without genetic manipulation.

The present invention relates to a composition for inducing the differentiation of stem cells into astrocytes, which contains CPI-455. It is expected that using the composition of the present invention will not only induce differentiation into astrocytes without using existing stem cell differentiation promoters, but will also enable the effective treatment of neurodegenerative diseases using the differentiated astrocytes.

The invention provides a method for amplifying norovirus by using an intestinal organ gas-liquid interaction model, and relates to the technical field of biological medicines. Comprising the following steps: preparing a culture medium, and inducing stem cell differentiation according to the prepared culture medium to form intestinal organs; placing the differentiated intestinal organs in Transwell for continuous culture, promoting maturation of the intestinal organs under the condition of a culture medium, and forming an intestinal organ gas-liquid interaction model; extracting the norovirus, infecting the intestinal organ gas-liquid interaction model by using the norovirus, and then detecting the amplification multiple of the norovirus. The intestinal organ gas-liquid interaction model prepared by the invention is similar to human intestinal cells in composition, can realize infection and amplification of the norovirus in vitro, can be repeatedly obtained in large quantities, avoids clinical risks and ethical constraints, and is suitable for research of norovirus in-vitro infection mechanism and drug screening.

This application relates to a method for inducing stem cells to differentiate into retinal pigment epithelial cells (RPE), comprising the following steps: culturing the pluripotent stem cells in a first culture medium before the pluripotent stem cells exhibit cell state decline after cell culture, wherein the first culture medium comprises DMEM medium, serum, β-mercaptoethanol, non-essential amino acids (NEAA), and L-glutamine. The method described in this application can significantly improve the efficiency of differentiation into RPE.

The presently disclosed subject matter provides for in vitro methods of inducing differentiation of stem cells (e.g., human stem cells) into somatotrophs, and differentiated cells generated by such methods. The presently disclosed subject matter also provides for uses of such cells for treating growth hormone deficiency.