143 results about "Vascularizes" patented technology

The invention discloses a bionic vascularized soft tissue with a multilayer vascular structure and a preparation method thereof. The preparation method comprises the steps of: employing a moulding process or 3D printing way to obtain a sacrificial membrane with a branched structure; coating or spraying a polymer solution containing a pore-foaming agent on the surface of the sacrificial membrane toobtain a polymer film; removing the treated sacrificial membrane of the structure and the pore-foaming agent to obtain a vascular channel-like structure with surface pores; conducting perfusion planting of endothelial cells in the channel of the vascular channel-like structure; planting smooth muscle cells or fibroblasts in the surface pores of the vascular channel-like structure; and placing thetreated vascular channel-like structure in a mold, adding an aquogel solution containing parenchymal cells, and conducting moulding so as to obtain the bionic vascularized soft tissue. The method provided by the invention can construct bionic blood vessels with a multilayer structure in artificial tissues.

The invention provides a method for building a hollow vascularized heart based on the 3D biological printing technology and the hollow vascularized heart and relates to the technical field of tissue engineering and biology. The method includes: performing reverse modeling to build a heart three-dimensional grid model, importing the data of the heart three-dimensional grid model into a double-nozzle biological printing machine, and driving the nozzles of the printing machine to move according to a predesigned CAD digital model and selected forming parameters, wherein the nozzle 1 of the printing machine is loaded with sacrificial materials and used for printing a support framework, the nozzle 2 of the printing machine sprays biological ink to obtain building bodies, and the effective components of the biological ink comprise hydrogel, platelet-rich plasma, third-generation human umbilical vein endothelial cells and SD rat primary cardiomyocytes; crosslinking and cleaning the building bodies, and performing three-dimensional culture to form the hollow vascularized heart. The method has the advantages that the problem that a large-size hollow vascularized heart is hard in integrated printing, and the hollow vascularized heart built by the method is high in cell activity and has certain functions.

The present invention involves the use of nitrone free radical trapping agents in the treatment and prevention of gliomas. The agents may be used alone or combined with other traditional chemo- and radiotherapies and surgery, to treat or prevent glioma occurrence, recurrence, spread, growth, metastasis, or vascularization.

The invention relates to the technical field of biological materials and clinical medicine, and provides an injection, a preparation method of the injection and application of the injection in dentalpulp regeneration. The injection comprises a composite preparation as a dispersion phase and a dispersion liquid as a continuous phase. The composite preparation comprises a drug, a microcarrier and stem cells, the drug is wrapped in the microcarrier, and the stem cells are attached to the surface of the microcarrier. The dispersion liquid is selected from at least one of a group consisting of a balanced salt solution, a serum-free culture medium and injectable hydrogel. The injection is mainly applied to regeneration of clinical functional dental pulp tissues, and regeneration of dental pulpin affected root canals is realized. By utilizing the injection, the formation of the nerve and vascularized dental pulp-like tissue in the root canal can be realized, the nutrition, defense and sensory functions of the dental pulp tissue are recovered, and the service life of the affected tooth is prolonged.

The invention relates to a regeneration material of artificial dermis substitution and a preparation method thereof. In the regeneration material of a dermis substitution for tissue engineering skin for loading rhGM-CSF, the regeneration material is formed by a heparinizing collagen-chitosan bracket loading the rhGM-CSF solution. The invention further discloses a preparation method of the regeneration material. The invention provides an artificial dermis substitution with good performance for curing the whole skin coloboma of trauma, burning, chronic skin ulcer, thereby being capable of remarkably quickening the vascularization process of the regeneration material of artificial dermis substitution, decreasing the infection risk, promoting the healing of the surface of a wound and reducing the hyperplasia of scar, and thus easing the pain of patients. The invention can be widely applied for the aspects of trauma, burning, surgery reshaping, and the like. The preparing method is simple, the source of the material is wide, the production efficiency is high, and the invention is suitable for industrial production.

The invention discloses a preparation method and an application of a biological scaffold material and SVF assistant adipose tissue. Under an aseptic condition, the biological scaffold material, a stromal vascular fraction (SVF) prepared by digesting purified adipose granules with a medical-graded collagenase, and the purified adipose particles are evenly mixed according to a certain proportion to prepare an adipose graft for filling. The SVF is a mixture of adipose mesenchymal stem cells and various cells, is transplanted after being mixed with autogenous adipose particles, and can be continued to be developed into a mature adipose tissue in vivo; the biological scaffold material can provide a support for transplanted adipose, allows the transplanted tissue to be prone to revascularization, and improves the survival rate of the transplanted tissue; the transplanted tissue is suitable for being injected with a 16 G or 18 G needle, facilitates clinical application, and can be used for repairing of concave deformity (such as tempora and cheek concave), beautifying packing of faces and chests, soft tissue function reconstruction and the like; and especially in the beautifying packing, the prepared transplanted adipose tissue can promote shape recovery and prevent liquefactive necrosis.

The invention relates to the field of stem cell induction culture, in particular to a culture method for induced differentiation of hair follicle stem cells into vascular endothelial cells. The culture method for induced differentiation of the hair follicle stem cells into the vascular endothelial cells comprises steps as follows: (1) separation of rat hair follicle stem cells; (2) culture of the rat hair follicle stem cells; (3) purification of the rat hair follicle stem cells; (4) culture of in-vitro induced differentiation of the rat hair follicle stem cells into the vascular endothelial cells. The vascular endothelial cells for promoting early vascularization of skin after trauma are obtained with the culture method, so that repair of traumatic skin is promoted; meanwhile, seed cell sources can be provided for solving problems of vascularization of tissue engineering skin, ischemic disease treatment through cell transplantation and the like.

The invention provides a vascularized tumor microfluidic organ chip used for culture in vitro, and a preparation method for the vascularized tumor microfluidic organ chip. The chip comprises a PMMA module and a glass sheet, wherein the PMMA module is provided with three through holes which penetrate through the thickness direction of the PMMA module; the periphery of the upper surface of the glasssheet and the periphery of the lower surface of the PMMA module are bonded into a whole through a bonding layer, and the three through holes independently form a first cavity used for containing a tumor sphere, a second cavity used for storing culture solution, and a third cavity; in addition, the middle area of the lower surface of the PMMA module and the glass sheet form a hollow tissue cavity;and a static pressure difference generated by the culture solution with a liquid level height difference can accelerate a three-dimensional blood capillary network to be generated, and through co-culture of the three-dimensional blood capillary network and the tumor sphere, blood capillaries can be collected and can be constructed into three-dimensional vascularized tumor micro-tissues. The chipovercomes the defects of time consumption and high cost when the microfluidic organ chip is manufactured at present, the chip has a simple technology, and an application value is provided especially for researching in vitro vascularized tumor diseases.

The invention relates to a method for constructing regenerated nerve vascularized bones, cartilages, joints or body surface organs by a prefabricated female die bracket. Firstly, the female die bracket of a defected tissue or organ is printed in a three-dimension manner by using a computer assisted design/manufacturing technology; then, a surface of a periosteum and/or perichondrium is formed in a body through an operation, the female die bracket is placed and fixedly sutured on a germinal layer of the autologous periosteum and/or perichondrium to form a closed space; after the bones, cartilages, joints or body surface organs whose male die shape corresponds to a female die are regenerated in the space constructed by the female die and the germinal layer of the surface of the periosteum and/or perichondrium, a newly regenerated tissue is taken out, the female die bracket is removed, and the tissue is transplanted to the position of the defected tissue or organ to reconstruct. The newly generated tissue or organ prepared through autologous prefabrication has rich new vessels and nerves, does not contain any exogenous materials participating in tissue regeneration, is high in safety, accurate in shape at the same time, and quite similar to the normal tissue or organ.

The invention relates to a vascularized tissue engineering bone and a preparation method thereof. The vascularized tissue engineering bone is composed of a support composed of poly-L-lysine (PLL) modified coralline hydroxyapatite (CHA) as well as a vascular endothelium dermatoblast patch and a bone formation cell patch which are sequentially wrapped at the inner layer of the support. According to the invention, an exogenous support obtained by virtue of the PLL modified CHA is beneficial to crawling ingrowth of blood vessels, and vascularization is realized finally, so that a precondition is provided for long term survival of cells in the tissue engineering bone, and organism immune rejection and inflammatory response are also reduced; adipose mesenchymal stem cells are selected for preparing a double cell patch, endothelial cells different in sources do not need to be added, cells are rich in resources and easy to separate and acquire, a trauma on a donor is minimal, ethical principles are not violated, and culture requirements are low. The vascularized tissue engineering bone provided by the invention is simple in preparation method and mild in preparation conditions, has good bone formation and vascularization properties, is consistent with in vivo application requirements, and has good application prospects as a novel vascularized tissue engineering bone.

The invention relates to the technical field of medical device accessories, and in particular relates to a hemostatic compressor for the cardiology department. The hemostatic compressor for the cardiology department is simple in structure; compression treatment of the affected part of a patient is carried out by using a GelMA hydrogel dressing; the additional burden of a medical staff is reduced;wound surface vascularisation and epithelial regeneration are accelerated; the wound surface healing speed is increased; the infection rate is reduced; and the living quality of the patient is improved. The hemostatic compressor for the cardiology department comprises a sickbed, a reciprocating screw, a limiting rod and a GelMA hydrogel dressing patch; a left platform is arranged at the left end of the sickbed; the top of the left platform is fixedly connected with a first rodless cylinder; the first rodless cylinder is glidingly provided with a sliding block; the top of the sliding block is fixedly connected with a left bracket; the top of the left bracket is fixedly connected with a second rodless cylinder; the second rodless cylinder is glidingly provided with a movable block; a first cylinder is arranged at the bottom of the movable block; the output end of the first cylinder is provided with a box, which is hollow; the inner bottom of the box is fixedly connected with two sets ofsupport rods; and both the two sets of support rods are each provided with a first bearing.

The invention discloses a silicon-doped hydroxyapatite based vascularization-promoting porous scaffold material, and a preparation method and application of the porous scaffold material, and belongs to the technical field of biological materials. The silicon-doped hydroxyapatite based vascularization-promoting porous scaffold material has a molecular formula of Ca<10>(PO4)<6-x>(SiO4)<x>(OH)<2-x>,wherein x is more than 0 and less than 0.75. The stent material provided by the invention has directional gradient pores which are in communication along the radial direction, and meanwhile, the porescommunicate with each other, so that cell adhesion and regeneration are facilitated, and whole-layer skin repair related cells grow into the center from the edge and the bottom of a wound. In addition, the scaffold material prepared by the preparation method also has good biocompatibility, controllable biodegradability and mechanical property, and has a wide application prospect.

The invention relates to a preparation method of a pre-vascularized biological structure body with a multi-scale channel network. The preparation method comprises the following specific steps: (1) preparing hydrogel microspheres; (2) preparing a sacrificial model; (3) preparing a biological structure body mold; and (4) preparing the biological structure. According to the method, the hydrogel microspheres are used as a basic construction unit of the biological structure body, and micro gaps between the spheres generated after the microspheres are stacked are combined with a macro-scale three-dimensional vascular network prepared by additive and subtractive composite forming, so that the controllable preparation of the pre-vascularized biological structure body with the three-dimensional multi-scale and high-density vascular network is realized.

The invention discloses a formula and a preparation method of a photocuring 3D printing haversian system artificial bone scaffold. The scaffold is prepared from the following raw materials: 2-5% of collagen (medical grade), 5-10% of normal saline, 43-61.5% of nano hydroxyapatite (medical grade), 20-40% of photosensitive resin (medical grade), 0.5-1% of photoinitiator and 1-3% of dispersant, wherein the sum of the weight percentages of the components is 100%. The preparation method of the photocuring 3D printing haversian system artificial bone scaffold is inspired by natural bone microstructure, uses a bionic method for designing an artificial bone internal pore structure, and promotes the vascularization formation in an artificial bone implant body so as to promote the bone repair process. The application of the 3D printing method realizes individualized customization of a bone defect part of a patient, can accurately control the appearance, the internal structure and the degradationrate of the artificial bone, really realizes customization, vascularization and intelligence of the artificial bone scaffold, and provides a new idea for design and development of the artificial bonescaffold.

The invention provides an artificial structure capable of being used for rapidly regenerating vascularized tissue and a construction method of the artificial structure. The artificial structure includes cell/hydrogel microspheres, tissue-related cells, vascularized cells, growth factor slow-release microspheres and a carrier material, wherein tissue-related cells are uniformly distributed in the cell/hydrogel microspheres, and the outsides of the microspheres contain a vascularized cell layer; and the cell/hydrogel microspheres, the tissue-related cells, the vascularized cells and the growth factor slow-release microspheres are uniformly distributed in the carrier material. The artificial structure provided by the invention can be cultured in a bioreactor to obtain a vascularized tissue precursor with a vascular-like network structure, or transplanted into the body to regenerate vascularized functional tissue. The obtained tissue precursor or functional tissue has certain physiologicalfunctions and can be used for in-vivo and in-vitro studies such as tissue development, disease occurrence, drug development and detection, tissue regeneration and repair.

Methods, computer programs, and systems for detecting disease in vasculature. The method includes obtaining images of the vasculature. The method includes extracting vessel measurements from the obtained images. The method includes determining features of the vasculature in the obtained images based on the extracted vessel measurements. The method includes applying artificial intelligence algorithms to determine if the disease is present in the vasculature based on these vascular features.