278 results about "Vascularizes" patented technology

The invention discloses a vascularized immune co-culture organoid chip for simulating a tumor microenvironment. The vascularized immune co-culture organoid chip comprises a chip main body and a bottom plate, a flow channel and a liquid storage tank are arranged in the chip main body; the runners comprise a first runner, a second runner, a third runner and a fourth runner; a compartment is arranged in the middle of the third flow channel; the first flow channel is connected with a first liquid storage tank which is used for injecting a first hydrogel suspension obtained by mixing fibroblasts and hydrogel into the first flow channel; the second flow channel is connected with a second liquid storage tank for injecting a culture solution into the second flow channel; a compartment is arranged in the middle of the third flow channel; the third flow channel is connected with a third liquid storage tank communicated with the compartment, and the compartment is connected with a compartment liquid storage tank; the compartment liquid storage tank is used for injecting a second hydrogel suspension obtained by mixing tumor organs and hydrogel into the compartment, and the third liquid storage tank is used for injecting hydrogel into the third flow channel; and the fourth flow channel is connected with a fourth liquid storage tank for injecting vascular endothelial cell and immune cell suspension into the fourth flow channel. The invention further discloses a vascularized immune co-culture organoid culture method for simulating the tumor microenvironment.

The invention provides a vascularized organ-like chip based on seal microstructure patterning and a preparation method thereof, the chip comprises a micro-channel layer, the upper surface of the micro-channel layer is provided with a gel perfusion channel, a central tissue chamber and culture medium flow channels at two sides which are connected, and tumor spheres or colon cells are inoculated in the central tissue chamber; the liquid storage pool layer is arranged on the upper surface of the micro-channel layer and corresponds to the inlet and outlet of the gel filling channel, the inlet and outlet of the central tissue chamber and the inlet and outlet of the culture medium runner, the liquid storage pool layer is provided with a plurality of liquid storage pools and a plurality of through holes, and the liquid storage pools contain culture solutions to provide nutrients for cultured tissues; and the seal module is arranged above the central tissue chamber, the seal module is provided with a tumor seal and a colon seal, and the tumor seal and the colon seal are respectively provided with a microstructure three-dimensional module and are respectively used for simulating the culture environment of tumor spheres or colon cells. According to the invention, in-vitro construction of the vascularized tumor model and the colon model is realized, and a guide is provided for construction of the vascularized organ-like model with specific organ three-dimensional microstructure characteristics.

The invention provides human placenta-derived vasogenic stem cells (hPASCs) and application thereof. The human placenta-derived angiogenic stem cells (hPASCs) disclosed by the invention are preserved in China Center for Type Culture Collection, Wuhan University, Wuhan, China; the preservation date is April 2, 2025; the preservation number of the strain is CCTCC (China Center For Type Culture Collection) NO: The hPASCs disclosed by the invention contain angiogenic cell subgroups, have stem cell stemness, have an angiogenesis effect in vivo and in vitro, and can be used for treatment of diseases such as cardiovascular diseases and vascularization construction of artificial organs and tissues.

The invention belongs to the technical field of organoid culture, and particularly relates to a co-differentiated vascularized liver organoid and a construction method thereof. Aiming at the problems that the existing liver organ vascularization mostly adopts a co-culture mode, the operation process is complicated, the cell source heterogeneity is relatively high, the randomness is strong, the uniformity is poor and the like, the invention provides the construction method of the co-differentiated vascularized liver organ. The preparation method comprises the following steps: sequentially inducing and culturing directional endoderm cells, posterior preintestinal cells and hepatic endoderm cells, adding an S1 culture medium containing VEGF to induce and culture for 1-3 days, then adding an S2 culture medium containing VEGF, BMP4 and FGF2 to induce and culture for 3-5 days, and then adding an S3 culture medium containing VEGF to induce and culture for 14-20 days to obtain the co-differentiated vascularized liver organ. According to the method disclosed by the invention, the operation process is simplified, the experiment efficiency is improved, the liver organ which is rich in blood vessel network, high in maturity and of a blood vessel-bile duct structure is obtained, and a foundation is laid for large-scale production and clinical application.

The invention provides a pourable tumor organoid vascularization model and a construction method thereof, and relates to the technical field of organoid culture. A temperature-sensitive degradable material and a photo-crosslinking modified temperature-sensitive material are used, the composite stent is printed through two channels, a multi-component gel mask partition curing process is combined, the stability of a perfusion pipeline is improved, and a partitioned highly-bionic complex microenvironment is accurately constructed in a macroscopic three-dimensional space; and constructing to obtain the perfusion tumor organ vascularization model. The model is high in bionic degree and stable in structure, can be applied to deepening cell and microenvironment interaction research and tumor-related drug screening, and has a good application prospect.

The invention discloses a vascularized tissue culture chip as well as a construction method and application thereof, and belongs to the technical field of biological tissue engineering and biological medicines. The vascularization tissue culture chip is formed by stacking five layers of sub-chips with different function requirements, a fluid channel and a culture chamber are formed, a highly integrated system is formed, and the design of a micro-column array further optimizes the fluid dynamic characteristics and reduces the dead angle effect of fluid. In-vivo microcirculation can be simulated, and a stable and controllable nutrition and metabolite exchange environment is provided for skin and liver tissue. The vascularized tissue culture chip provided by the invention provides a powerful research platform for a plurality of biomedical research fields such as personalized medical treatment, disease modeling, drug screening, toxicity testing, disease model research and the like.

Aiming at the challenge of osteochondral defect repair, the invention provides a preparation method of a fiber-reinforced self-repairing hydrogel scaffold (SBPS). According to the method, phenylboronic acid modified sodium alginate, polyvinyl alcohol and fibroin nanofibers rich in beta-Sheet are utilized to form the hydrogel through dynamic boric acid ester bonds and hydrogen bonds. The SBPS hydrogel loaded with PDGF-BB is combined with the nanosheet containing LDH / ChS / TGF-beta3, so that multi-dimensional synergy of mechanical strengthening, sequential drug release (PDGF-BB is released in an early stage and TGF-beta3 / Mg < 2 + > is released in a later stage), ROS removal and endogenous repair is realized. The Mg < 2 + > concentration gradient effect further assists staged regulation (vascularized bone regeneration-cartilage matrix synthesis), and breaks through the limitation of a single factor. Experiments prove that the scaffold can effectively drive angiogenesis, stem cell recruitment and cartilage / bone differentiation in vivo and in vitro, and finally osteochondral regeneration with structure-function matching is achieved.

The invention discloses a bone regeneration promoting drug-loaded skull implant with a bionic structure and a preparation method of the bone regeneration promoting drug-loaded skull implant, and belongs to the technical field of medical materials. The skull implant is prepared through a flat plate mold pressing technology and is of a bionic three-layer structure, the upper layer and the lower layer of the haft tube imitating structures and the middle layer of the bone trabecula imitating structure are prepared through the water-soluble core, a vascularization channel can be provided, and bone tissue climbing is promoted; a polydopamine-carboxymethyl chitosan coating is used for carrying out surface modification on a porous structure of the implant, hydroxyapatite in-situ forming and bone regeneration promoting medicine loading are achieved through an impregnation process, and the polydopamine coating can provide a hydration environment; hydroxyapatite in-situ forming increases biocompatibility on the premise of not influencing the mechanical properties of implants, in addition, hydroxyapatite degradation and drug release can jointly induce bone tissue regeneration, and skull repair is promoted.

The invention relates to the technical field of tumor biomedical engineering and organ chips, in particular to a vascularized gastric cancer organ chip and a preparation method thereof.The chip is composed of an integrated micro-fluidic main body, an annular micro-column array and an optical sealing film, and a central culture cavity is divided into a tumor area and a blood vessel area by micro-columns; the preparation method comprises the following steps: injecting a fibrous protein solution containing human umbilical vein endothelial cells and cancer-related fibroblasts into a vascular region, and adding thrombin for in-situ gelation to form a pre-vascularized network; the method comprises the following steps: mixing a patient-derived gastric cancer organ with a methacrylic acid esterified gelatin pre-polymerized solution, injecting the mixture into a tumor area, and carrying out photo-crosslinking immobilization; culturing for 7-14 days under the dynamic perfusion condition of a mixed culture medium to obtain a three-dimensional gastric cancer organ model containing a capillary network; the method is expected to be used for rapid, low-cost and high-throughput screening of chemotherapy or anti-angiogenesis drugs and accurate prediction of individual curative effects of patients.

The invention provides a covered stent coated with an electrostatic spinning membrane and a preparation method thereof.The covered stent comprises a metal stent and a covering membrane, the metal stent is composed of a spiral metal wire, the metal wire is provided with a plurality of zigzag parts which are evenly arranged and connected end to end, every two adjacent zigzag parts are in a wave crest shape and a wave trough shape respectively, and the multiple zigzag parts enable the metal wire to be in a wave shape; the covering film covers the surface of the metal support to form a tubular structure, and the tubular structure can be kept shaped after bending deformation at the lesion position so that the tubular structure can be matched with the lesion position in shape. The covered stent has a high specific surface area and an adjustable porous structure, the micron-sized aperture of the covered stent can effectively prevent blood permeation, the high porosity allows a proper amount of tissue or cells to penetrate, vascularization and tissue integration of the implanted stent are promoted, and therefore the risk of stent displacement or instability is reduced.

The invention belongs to the technical field of organoid culture, and particularly relates to a co-differentiated vascularized islet organoid and a construction method thereof. Aiming at the problems that the existing pancreatic islet organ vascularization mostly adopts a co-culture mode, the operation process is long, the difficulty is high, the randomness is realized, the uniformity is poor and the like, the invention provides the construction method of the co-differentiated vascularized pancreatic islet organ. The method comprises the following steps of: sequentially inducing and culturing directional endoderm cells, original intestinal canal cells and posterior preintestinal cells, and adding a culture medium containing VEGF (vascular endothelial growth factor), BMP4 (bone morphogenetic protein 4) and FGF2 (fibroblast growth factor 2) for culturing in subsequent induced differentiation, so as to obtain the co-differentiated vascularized islet organ. Under the condition that exogenous endothelial cells are not added, the iPS cells are directionally differentiated to generate the vascularized islet organ containing beta, alpha and delta cells and vascular endothelial cells for the first time, the vascularized islet organ is more mature than non-vascularized islet organs and has a stronger insulin secretion function, and a new thought and an experimental basis are provided for realizing functional healing of diabetes mellitus.

An inflated and suspension dried decellularized extracellular matrix of a mammalian organ or tissue, or a vascularized portion thereof, and methods of making and using the inflated and suspension dried material, are provided.

The invention belongs to the technical field of biomedical engineering and microfluidics, and particularly discloses a vascularized organ chip and method for large-scale culture, the vascularized organ chip comprises an upper runner layer and a lower culture layer, and the runner layer and the culture layer are fixedly sealed; a liquid storage pipe matched with the flow channel layer is adhered to the upper surface of the flow channel layer; according to the vascularized organ chip, the flow channel layer is used for pouring a culture solution to provide fluid stimulation and nutrients for cells; the three parallel-connected large-scale culture chambers of the culture layer are used for culturing cells, so that blood circulation of a microvascular system in a human body can be simulated, and a microenvironment in tissues can be reproduced more truly; meanwhile, the requirement for culturing a large number of tissue cells through the chip can be met, and the cell yield and the experiment scale can be effectively increased.

The invention relates to a two-phase hydrogel system based on space-time gradient regulation and control as well as a preparation method and application of the two-phase hydrogel system. The biphase hydrogel system can also be expressed as a GelMA composite hydrogel scaffold of a porous structure, the whole biphase hydrogel system is of a core-shell structure, the inner layer core mineralizes SilMA hydrogel microspheres, the porous GelMA hydrogel scaffold is used as an outer layer, and the angiogenesis promoting factor dimethyl oxalaminoacetic acid, the human umbilical vein endothelial cells and the bone marrow mesenchymal stem cells are loaded on the outer layer. Wherein the inner-layer core mineralized SilMA hydrogel microspheres realize continuous release of osteogenesis signals by slowly releasing nHAp, and the outer-layer porous GelMA hydrogel stent releases DMOG by rapid degradation to realize early angiogenesis, so that space-time coupling construction of blood vessels and bone tissues is realized. Through differential degradation characteristics of inner and outer layers of hydrogel, precise time sequence cooperative regulation and control of angiogenesis and bone tissue remodeling can be realized, and the bionic property and function integration capability of a vascularized bone organ structure can be improved, so that the clinical transformation potential of the vascularized bone organ structure is improved.

The invention discloses an artificial auricle material compounded with a vascularization and cartilage double framework and a preparation method of the artificial auricle material. Comprising the following steps: 1) performing 3D modeling as required to obtain an artificial auricle macroporous stent model, and presetting an angiogenic differentiation framework on the artificial auricle macroporous stent model; 2) based on a multi-material multi-nozzle photocuring 3D printing technology, using a GelMA solution to print an artificial auricle macroporous stent, using a mesenchymal stem cell (MSCs)-loaded GelMA solution to print a vascularized framework in a framework, and after angiogenesis differentiation culture, obtaining an intermediate form artificial auricle compounded with the vascularized framework; and (3) introducing a ColMA solution loaded with MSCs into pores of the artificial auricle in the middle form by combining a multi-time dipping-photocuring method, and carrying out chondrogenic differentiation culture to obtain the artificial auricle material compounded with the vascularization and chondrogenesis dual frameworks at the same time. A vascularization framework and a cartilage framework are arranged in the stent at the same time, and a design normal form is provided for an artificial auricle with a complete physiological function.

The invention discloses a bionic heterogeneous composite structure bone implant and a preparation method thereof, and relates to the technical field of medical implants.The bionic heterogeneous composite structure bone implant comprises an inner-layer cross-shaped structure, a middle-layer negative Poisson's ratio structure and an outer-layer honeycomb structure, and the cross-shaped structure, the negative Poisson's ratio structure and the honeycomb structure jointly form a bionic composite structure; and immersing the bionic composite structure subjected to double-network crosslinking into a drug-containing solution for post-treatment and drug loading to obtain the bionic heterogeneous composite structure. According to the invention, bionic adaptation and intelligent optimization of mechanical properties are realized, and the stress shielding problem is solved; according to the invention, osseointegration and vascularization processes are obviously accelerated and enhanced, and leap-type improvement of biological activity is realized; a novel intelligent treatment mode based on in-vivo microenvironment response is developed, and on-demand and accurate drug delivery is achieved.

Devices, systems, and methods for causing retrograde oxygenated blood flow in at least a portion of the venous system of the myocardium are disclosed. Devices and methods include a method for causing retrograde oxygenated blood flow in at least a portion of the venous system of the myocardium, the method comprising: implanting a variable occluder in a coronary sinus region proximate a left atrium, wherein the variable occluder is configured to gradually increase a level of blood flow restriction over a period of days to enable a venous system of the myocardium to compensate for increased pressure caused by the gradual increase in the level of blood flow restriction; and following venous system compensation, shunting oxygenated blood flow into the coronary sinus to cause retrograde flow of oxygenated blood in at least a portion of the venous system of the myocardium to thereby enable revascularization of the myocardium.

The invention belongs to the field of biomedicine, and particularly relates to a 3D bio-printed vascularization-promoting bladder patch and a preparation method thereof. The preparation method comprises the following steps: polymerizing prepared GelMA and Laponite to prepare GelMA-Laponite hydrogel, then mixing and printing the GelMA-Laponite hydrogel and seed cells on a bladder acellular matrix by using a 3D biological printing machine to form a GelMA-Laponite stent with a three-dimensional space structure, and crosslinking the stent by using ultraviolet light to obtain the double-layer vascularized bladder patch. The construction level of the tissue-engineered bladder patch is expected to be improved, the vascularization problem of the tissue-engineered bladder graft is solved, and a good theoretical basis and technical support are laid for promoting the clinical application process of bladder tissue engineering.

The invention provides a vascularized bone organ and a construction method thereof, and a vascularized bone organ model containing a hollow vascular network is constructed by combining a suspension 3D biological printing method, a cell-carrying printing strategy and a sacrificial biological ink material. The vascularized bone organ model has excellent structural stability, mechanical performance, growth and development capability and osteogenesis capability, selected materials and a manufacturing method are simple in process, engineering stable production can be achieved, cells can be blended for a long time for three-dimensional culture, and the vascularized bone organ model has high applicability and research potential.

The invention relates to the field of medical dressings, in particular to a biodegradable tissue repair functional dressing and a preparation method thereof. The invention relates to a biodegradable tissue repair dressing, in particular to a chronic wound dressing (also known as a biodegradable tissue repair dressing) composed of a degradable electrostatic spinning functional film layer and an absorption layer (with or without a breathable film), and a preparation method and application of the biodegradable tissue repair dressing. The hydrogel can promote cell migration and proliferation, tissue growth and vascularization, has high wound seepage absorbability and air permeability, and provides a new method for treating acute and chronic wounds.

Disclosed in the present invention are a biomimetic vascularized iPSC-hep spheroid for liver regeneration, and a preparation method therefor. The biomimetic vascularized iPSC-hep spheroid is a microcapsule with a porous hyaluronic acid methacryloyl as a shell and a human-induced pluripotent stem-cell-derived hepatocyte spheroid as an inner phase, and human umbilical vein endothelial cells adhere to the surface of the shell. The biomimetic vascularized iPSC-hep spheroid of the present invention promotes effective communication between hiPSC-heps and HUVECs, and provides barrier protection for an encapsulated hiPSC-hep spheroid so as to avoid attack by the immune system. Compared with dispersed cells, the cell-to-cell interaction in the cell spheroid improves cell-to-cell communication, helps to simulate the physiological heterogeneous structure and cell microenvironment of the liver, and can more effectively repair an acutely failing liver.

The invention discloses a vascularized skin chip, a vascularized skin model and application of the vascularized skin chip and the vascularized skin model. The material of the vascularized skin chip does not contain polydimethylsiloxane; the vascularized skin chip comprises a dermal culture chip layer and an epidermal culture chip layer from bottom to top, wherein a vertically through culture cavity is formed between the dermal culture chip layer and the epidermal culture chip layer; the dermal culture chip layer is provided with a capillary channel, and the capillary channel is used for constructing a capillary in the dermal layer. According to the vascularized skin chip, dermis and epidermis can make direct contact, interaction between cells is truly simulated, the activity and functions of the cells are kept, good mechanical support is provided for the epidermis and the dermis layer, and cell growth and tissue form maturation are promoted. The microvessel channel can simulate microvessels of a corium layer and is used for simulating supply of nutrient substances and excretion of metabolic waste, a dynamic fluid environment is provided, and the skin structure and the microenvironment are restored more truly.

The invention belongs to the technical field of oral implantation and bone defect repair, and provides a biodegradable zinc-based GBR membrane as well as a preparation method and application thereof. The method comprises the following steps: mixing a zinc alloy, a biodegradable polymer material and a solvent to obtain a mixed solution; drying the mixed solution to obtain a composite membrane; performing first melt extrusion on the composite film to obtain first composite filaments; performing second melt extrusion on the first composite filaments to obtain second composite filaments; the second composite filaments are subjected to 3D printing, and the biodegradable zinc-based GBR film is obtained. According to the biodegradable zinc-based GBR membrane, the zinc alloy and the biodegradable high polymer material are compounded to form a membrane with a double-layer structure, and the biodegradable zinc-based GBR membrane not only has good biocompatibility and biodegradability, but also has the potential of promoting bone tissue regeneration and vascularization, so that the limitation of a traditional GBR membrane is effectively solved; and a new solution is provided for oral implantation and bone defect repair.

The invention belongs to the field of biomedical materials and manufacturing thereof, and discloses a composite 3D printing stent with a time sequence regulation blood vessel and bone tissue regeneration function as well as a preparation method and application of the composite 3D printing stent. The method comprises the following steps: respectively loading an osteogenesis inducer and a vascular induced differentiation agent into two carrier materials with different degradation rates, and carrying out 3D printing and gel coating modification according to a reverse sequence of a physiological repair process of carrying out vascularization first and then carrying out osteogenesis to form a composite 3D printing stent of which the inner layer is loaded with the osteogenesis inducer and the outer layer is loaded with the vascular induced differentiation agent. After the composite 3D printing stent is implanted into a body, according to different degradation rates of the composite material, the repair and regeneration process of vascularization and osteogenesis is realized in a manner of degrading the gel coating and degrading the 3D printing stent, and the composite 3D printing stent has good living compatibility, biological activity and mechanical properties, and has good application prospects. The material can be used as a biomedical material for repairing or treating bone defects or self-repairing, and has a good application prospect in treatment and self-repairing of bone defects.

The described system designed to promote the biological attachment of fibrous connective tissue to a bone implant features a bone implant with a scaffold defining a porous attachment surface, a suction port, and a manifold that distributes a negative pressure gradient across the attachment surface. The system also includes an extracorporeal vacuum pump and a percutaneous suction hose connecting the suction port of the implant to the vacuum pump. The implant is placed in the patient's body with connective tissue applied over the attachment surface, and the suction hose is attached and connected to the vacuum pump. The vacuum pump, operating outside the body, creates a negative pressure gradient over an extended period, promoting the growth of slow growing Sharpey's fibres for superior biological integration. A highly vascularised tissue graft, such as a pedicled muscle flap, may be applied adjacent to the avascular tendon to enhance endogenous growth factor migration.

Disclosed herein are organoid models of the blood-brain barrier, optionally having a disease associated feature, such as a cerebral cavernous malformation (CCM)-like feature, compositions comprising the same, methods for making the same from pluripotent stem cells, and methods of using the same. These organoids exhibit the complex organization of cells including neurons, endothelial cells, glial cells, and pericytes resembling the natural blood-brain barrier structure. The organoids may also exhibit disease like characteristics, such as having a cerebral cavernous malformation (CCM)-like features. These organoids may be used for studying the functions of the blood-brain barrier and cerebrovascular disorders.

The invention discloses an extracellular matrix biological dressing and a preparation method thereof, and the preparation method of the extracellular matrix biological dressing comprises the following steps: removing cells and immunogenicity of pretreated organs and vascularized tissues by using a detergent until the DNA content is less than 100 ng / mg dry weight and the alpha-Gal clearance rate is greater than 90%; putting into a solution containing growth factors for treatment; the treated organ and the vascularized tissue are cross-linked by adopting a chemical reagent, after cleaning, the organ and the vascularized tissue are treated by adopting glycerol and an end-capping reagent, moisture is removed, after sterilization treatment, the acellular matrix biological dressing is obtained, the volume ratio of the organ after moisture removal to the organ before moisture removal is 0.5: 1-1: 1, and the pore size of the organ and the vascularized tissue is 15-500 microns.

A challenge to the clinical viability of large-scale tissue engineering is being able to perfuse entire constructs with blood supply that persists long-term. To accomplish this, tissue engineering needs to replicate the hierarchically branching networks of microvasculature that distribute homogeneous blood supply from arteries to capillary beds in natural tissues. Chaotic Printing can be adapted to extruding wide sheets of hydrogel containing hierarchically branching layers of multiple cell types that are native to vascular tissue. An in vitro "prevascularization" period can allow these cells to form into hollow tubular structures that mimic native microvasculature and can be perfused with blood supply. These prevascularized sheets can then be implanted in a subject to integrate with native arteries and veins and perfuse injured tissue with new blood supply.

The invention discloses a bionic vascularized iPSC (induced pluripotent stem cell)-hepatocyte sphere for liver regeneration and a preparation method of the bionic vascularized iPSC-hepatocyte sphere. The bionic vascularized iPSC-hepatocyte sphere is a microcapsule which takes porous methacrylated hyaluronic acid as a shell and takes a human induced pluripotent stem cell derived hepatocyte sphere as an inner phase, human umbilical vein endothelial cells are adhered to the surface of the shell. According to the bionic vascularized iPSC-hepatocyte sphere, effective communication between hiPSC-heps and HUVECs is promoted, barrier protection is provided for the packaged hiPSC-hep sphere, attack of an immune system is avoided, and compared with dispersed cells, communication between cells is improved through interaction between the cells in the cell sphere, so that the service life of the hiPSC-heps and the HUVECs is prolonged, and the service life of the hiPSC-heps and the HUVECs is prolonged. The simulation of a physiological heterogeneity structure and a cell microenvironment of the liver is facilitated, and the acute failure liver can be more effectively repaired.

The invention provides in-situ forming fluid bionic hydrogel as well as a preparation method and application thereof, and belongs to the technical field of biological medicines. The invention provides an injectable GelSSO / PDA (at) SDF fluid bionic niche, after in-situ photo-crosslinking, preferential and sustained release of PDA (at) SDF NPs initiates early signal amplification, which recruits EPCs and MSCs through an SDF-1 alpha / CXCR4 axis, promotes activation of the angiogenic vascular niche through an AKT signal, and repolarizes macrophages to a regenerated M2 phenotype. Subsequently, the gradual degradation of the bionic niche triggers the stable release of SSO, and the later signal is further amplified to form an osteogenic niche with transcriptional activity, thereby maintaining the MAPK / ERK-mediated MSCs osteogenic differentiation. The fluid bionic niche can form a niche conforming to defects through photopolymerization, the local immune imbalance and endogenous progenitor cell recruitment are corrected in the early stage, then formation of new vessels and lamellar bones is promoted, and finally vascularization regeneration of diabetic skull defects is achieved.