81 results about "Vascularizes" patented technology

The invention provides a pourable tumor organoid vascularization model and a construction method thereof, and relates to the technical field of organoid culture. A temperature-sensitive degradable material and a photo-crosslinking modified temperature-sensitive material are used, the composite stent is printed through two channels, a multi-component gel mask partition curing process is combined, the stability of a perfusion pipeline is improved, and a partitioned highly-bionic complex microenvironment is accurately constructed in a macroscopic three-dimensional space; and constructing to obtain the perfusion tumor organ vascularization model. The model is high in bionic degree and stable in structure, can be applied to deepening cell and microenvironment interaction research and tumor-related drug screening, and has a good application prospect.

Disclosed in the present invention are a biomimetic vascularized iPSC-hep spheroid for liver regeneration, and a preparation method therefor. The biomimetic vascularized iPSC-hep spheroid is a microcapsule with a porous hyaluronic acid methacryloyl as a shell and a human-induced pluripotent stem-cell-derived hepatocyte spheroid as an inner phase, and human umbilical vein endothelial cells adhere to the surface of the shell. The biomimetic vascularized iPSC-hep spheroid of the present invention promotes effective communication between hiPSC-heps and HUVECs, and provides barrier protection for an encapsulated hiPSC-hep spheroid so as to avoid attack by the immune system. Compared with dispersed cells, the cell-to-cell interaction in the cell spheroid improves cell-to-cell communication, helps to simulate the physiological heterogeneous structure and cell microenvironment of the liver, and can more effectively repair an acutely failing liver.

The invention belongs to the field of biomedical materials and manufacturing thereof, and discloses a composite 3D printing stent with a time sequence regulation blood vessel and bone tissue regeneration function as well as a preparation method and application of the composite 3D printing stent. The method comprises the following steps: respectively loading an osteogenesis inducer and a vascular induced differentiation agent into two carrier materials with different degradation rates, and carrying out 3D printing and gel coating modification according to a reverse sequence of a physiological repair process of carrying out vascularization first and then carrying out osteogenesis to form a composite 3D printing stent of which the inner layer is loaded with the osteogenesis inducer and the outer layer is loaded with the vascular induced differentiation agent. After the composite 3D printing stent is implanted into a body, according to different degradation rates of the composite material, the repair and regeneration process of vascularization and osteogenesis is realized in a manner of degrading the gel coating and degrading the 3D printing stent, and the composite 3D printing stent has good living compatibility, biological activity and mechanical properties, and has good application prospects. The material can be used as a biomedical material for repairing or treating bone defects or self-repairing, and has a good application prospect in treatment and self-repairing of bone defects.

The invention provides in-situ forming fluid bionic hydrogel as well as a preparation method and application thereof, and belongs to the technical field of biological medicines. The invention provides an injectable GelSSO / PDA (at) SDF fluid bionic niche, after in-situ photo-crosslinking, preferential and sustained release of PDA (at) SDF NPs initiates early signal amplification, which recruits EPCs and MSCs through an SDF-1 alpha / CXCR4 axis, promotes activation of the angiogenic vascular niche through an AKT signal, and repolarizes macrophages to a regenerated M2 phenotype. Subsequently, the gradual degradation of the bionic niche triggers the stable release of SSO, and the later signal is further amplified to form an osteogenic niche with transcriptional activity, thereby maintaining the MAPK / ERK-mediated MSCs osteogenic differentiation. The fluid bionic niche can form a niche conforming to defects through photopolymerization, the local immune imbalance and endogenous progenitor cell recruitment are corrected in the early stage, then formation of new vessels and lamellar bones is promoted, and finally vascularization regeneration of diabetic skull defects is achieved.

The invention discloses a titanium-magnesium hollow screw system capable of promoting fracture healing and a manufacturing method. The titanium-magnesium hollow screw system comprises a titanium-based supporting structure and a magnesium filling layer. The titanium-based supporting structure comprises a main body part, the surface of the main body part is provided with an imitated bone trabecula part, and a plurality of bone gaps are formed in the imitated bone trabecula part; wherein bone gaps are filled with the magnesium filling layer, and the magnesium filling layer is combined with the titanium-based supporting structure in a physical embedding mode; the titanium-based supporting structure bears and maintains initial fixation and long-term bearing, so that the strength and the stability of the screw are ensured; the surface imitated bone trabecula and the communicated bone gaps increase the surface area and provide a physical channel for vascularization and bone tissue ingrowth, so that synostosis is promoted; the magnesium filling layer releases magnesium ions after being locally controllably degraded, so that osteogenesis and angiogenesis are favorably stimulated, the local biological environment is improved, instant mechanical support after an operation is ensured, bone healing is promoted through local biological activity release and structural orientation, the healing time is favorably shortened, and the healing quality is favorably improved.

The disclosure relates to compositions useful for ligament and tendon repair and methods of preparing the same. The disclosure is particularly suitable for repair of ligament ruptures, for example, anterior cruciate ligament ruptures. In an aspect of the invention, the compositions comprise a biocompatible clay, a polymer, a polypeptide, a calcium-based mineral that form a scaffold, as well as a bioabsorbable reinforcement structure incorporated thereon and / or therein. Methods of use and manufacture of the compositions, as well as kits including the compositions, are also provided.

The application provides a alginate-based aerogel, a preparation method and application thereof. The alginate-based aerogel has parallelly arranged large pores and oriented channels at an angle with the large pores, and the angle ranges from 0 to 180 degrees. The large pores are distributed in the same direction inside the alginate-based aerogel, and the oriented channels improve the interconnectivity between the large pores. The existence of the large pores and the oriented channels makes the alginate-based aerogel have the characteristics of anisotropy and a multi-scale pore structure, and the size of the large pores and the oriented channels is suitable for inducing vascularization and osteogenesis. Therefore, the aerogel can be used as a bone scaffold material. Tests show that the aerogel has the characteristics of light weight and high strength. In addition, other components beneficial to osteogenesis can be added in the alginate system, and the size of the pores can be adjusted without affecting the formation of the pore structure.

The present application relates to a kind of pluripotent stem cell co-differentiation method for generating vascularized heart organoids, the method comprises: (1) in iPSCs, obtain iEC-iPSCs by transferring exogenous gene Tet 3G and ETV2;(2) the iEC-iPSCs in step (1) are mixed with iPSCs, add culture medium I, prepare blastula after culture;(3) the blastula in step (2) is induced by using the heart organoid differentiation method combined with doxycycline, and vascularized heart organoids are prepared after differentiation culture.The present application uses Tet-on 3G inducible expression system, promotes the differentiation of part of cells in heart organoids to endothelial cell lineage by regulating the expression of ETV2 gene in part of iPSCs in blastula, and then forms vascularized heart organoids.

The present invention relates to systems, compositions and methods for multicellular bioprinting vascularized tissue constructs comprising a hierarchical vascular network. The methods employ a dual bio-ink strategy involving fibrous microgel matrix bio-inks with adjustable hardness, self-healing rheology, and microporosity to support capillary self-assembly, and sacrificial bio-inks that define a large pourable channel after removal. The system integrates top-down printing and bottom-up cell self-organization within a programmable bio-printing platform. The key aspect of the invention is to engineer the physical topography of the matrix to actively guide vascular morphogenesis beyond conventional permissive stents. The integrated platform enables the manufacture of complex bionic vascular networks with enhanced mass delivery, cellular viability, and in vivo integration potential. The invention represents an important progress in the aspect of extendable production of functional human tissues, and has application in regenerative medicine, disease modeling and drug discovery.

The application discloses a kind of biomimetic silk fibroin cartilage scaffolds for cranium defect repair, belong to biomedical engineering technical field.To solve the problem of single structure, low bone formation efficiency and mismatching of mechanical properties and degradation rate of existing cranium repair scaffold, the application provides a kind of scaffold, which includes in order from outside to inside: biomimetic periosteum layer, bone conduction and vascularization layer and cartilage induction layer.Biomimetic periosteum layer is dense nanofiber membrane structure, and plays the role of physical barrier and osteoinduction;Bone conduction and vascularization layer is gradient porous structure with gradually decreasing aperture from outside to inside, aiming to guide the ordered growth of blood vessels and cells, and promote rapid vascularization;Cartilage induction layer is sponge-like microporous structure, used for forming stable cartilage template and starting endochondral ossification.The application simulates the natural bone healing process through structural biomimicry and functional zoning, synergistically promotes vascularization and osteogenesis, and realizes efficient biological repair of cranium defect.

Described herein are three-dimensional scaffold compositions and methods for bone repair or regeneration. In some embodiments, the disclosed scaffolds comprise a core portion mimicking a native trabecular bone structure that is surrounded by an exterior portion mimicking a native cortical bone structure. In some embodiments, the scaffolds may be functionalized by mineralization and / or pre-vascularization to promote bone and blood vessel formation.

This invention relates to the field of organoid culture technology, specifically to a vascularized tumor organoid chip. It redesigns the traditional micropillar-separated culture medium and cell culture channels into a dynamically separated structure using acupuncture needles. This avoids the physical limitations of traditional micropillars on blood vessel growth, significantly increases the contact area between vascular cells, and promotes the growth of the vascular network. Simultaneously, the needle insertion channel and reservoir allow for free switching between micro-infusion pump and gravitational potential energy delivery modes. Furthermore, it enables dynamic detection during the co-culture of blood vessels and tumor organoids.

Biologic matrices designed to facilitate wound healing and related methods are disclosed. A matrix comprises a three-dimensional, resorbable collagen scaffold derived from a vascularized portion of a perfusion-decellularized mammalian organ or tissue. The scaffold features a porous structure with a plurality of interconnected pores originating from one or more outer surfaces, which are defined by intact vascular pathways of the perfusion decellularized mammalian organ or tissue. The porous configuration substantially enhances the scaffold's effective surface area, making it at least 5 times greater than that of a non-porous scaffold having equivalent outer dimensions. In certain embodiments, the surface area considering the interconnected pores can be 10 times larger, 15 times larger, or more than the non-porous scaffold of equivalent outer dimensions. The density of the scaffold can range from 2 milligrams per cubic centimeter to 20 milligrams per cubic centimeter, inclusive, further optimizing the matrix's performance for wound healing applications.

The invention provides a surface-modified biological material and a preparation method and application thereof, the surface-modified biological material has a structure of M-(P) n-L-X (formula I), M is a biological material matrix, P is a pre-modified layer, L is a linker, X is a chemical part for promoting repair, n is 0 or 1, n = 0 represents that the material does not have the pre-modified layer P, and n = 1 represents that the material has the pre-modified layer P. The surface modified biological material disclosed by the invention can efficiently and continuously initiate remarkable intracellular calcium ion internal flow, so that osteogenic differentiation, angiogenesis and the like are systematically promoted, and a new material solution is provided for the fields of bone defect repair, vascularization and the like which need precise mechanical signal regulation and control.

This disclosure provides compositions comprising engineered vascularized kidney tissues and methods of making vascularized glomerular kidney tissues. Also provided herein are in vitro kidneys comprising vascularized kidney tissues. Methods of making the compositions and in vitro kidneys and uses thereof are disclosed herein.

The invention relates to a construction method of a perfusion vascularized cervical cancer organoid chip, and relates to the technical field of biology. Comprising the following steps: S1, constructing a cervical cancer organ; s2, mixing the cervical cancer organ small cell cluster, the human umbilical vein endothelial cells and the human embryonic lung fibroblasts in proportion, and centrifugally gathering the mixed cells in a low-adsorption U-shaped plate to form a pre-vascularized cervical cancer organ; s3, re-suspending the pre-vascularized cervical cancer organoid and the human umbilical vein endothelial cells in the fibrous protein hydrogel, transferring the pre-vascularized cervical cancer organoid and the human umbilical vein endothelial cells into the organoid chip, and adding a vascularized cervical cancer organoid culture medium for culture, so that the endothelial cells form perfused blood vessels, and finally obtaining the perfused vascularized cervical cancer organoid chip. The prepared perfusion vascularized cervical cancer organoid can well solve the in-vitro vascularization problem of the organoid, simulates the in-vivo tumor angiogenesis process, is close to the in-vivo real tumor microenvironment, and provides technical support for exploration of cervical cancer disease mechanisms and related treatment.

This invention belongs to the field of biomaterials technology, specifically relating to a porous metal-ceramic composite scaffold for bone repair in diabetes and its preparation method. This invention uses tetrapodized zinc oxide as a three-dimensional stable pore-forming template, constructing an overall preparation route of "high-energy ball milling – t-ZnO template – SPS – etching," achieving uniform composite of high-melting-point metal and bioactive ceramic. This results in a porous metal-ceramic composite scaffold with uniform tissue structure, strong interfacial bonding, controllable pore structure, adjustable mechanical properties, and multiple bioactivities including osteogenic, immunogenic, and vascularization activities, significantly improving post-implantation bone integration efficiency and biofunctionality.

This invention discloses a method for constructing lung organoids with spontaneous vascularization and branching morphology. By optimizing the culture protocol and progressively inducing directional mesoderm, vascularized foregut endoderm, vascularized lung bud organoids, and vascularized lung organoids, lung organoids with both vascularization and branching morphology are successfully cultured, thus more realistically simulating the structural characteristics of the human lung. This invention has significant advantages: First, it can effectively induce the formation of vascularization and branching structures in lung organoids, overcoming the shortcomings of traditional lung organoids that lack vascularization and branching structures; second, by adjusting the culture method in the early stages, the uniformity of the lung organoids is significantly improved, enhancing the reliability and reproducibility of experimental results. This invention provides a more precise and efficient experimental platform for preclinical studies such as lung disease research, drug screening, and toxicology testing, and has significant application value.

Systems and methods for post-PCI coronary analysis. An example method includes accessing a plurality of angiographic images depicting at least one vessel of a patient's heart, the angiographic images including two or more post-percutaneous coronary intervention (post-PCI) images and one or more diagnostic images, wherein the post-PCI images depict a PCI location in which a PCI is included to revascularize a lesion, and wherein the diagnostic images depict the lesion; receiving, via a user interface presenting the post-PCI images, user input specifying positions of the PCI location in the post-PCI images; determining an index indicative of vascular function based on the angiographic images, wherein the index is derived based on a three-dimensional model of the portion of the patient's heart, and wherein individual portions of the diagnostic images depicting the lesion are masked during generation of three-dimensional model; and presenting, via the user interface, summary information associated with PCI, the summary information including at least the determined index.

The invention belongs to the technical field of biomedical engineering and microfluidics, and particularly relates to a vascularized organ chip capable of collecting multi-cell co-culture or organoid metabolites in situ for multiple times. The invention provides a vascularized organ chip. The vascularized organ chip sequentially comprises a runner layer, a cell culture layer, a porous membrane layer and a metabolite collection layer, the runner layer is provided with a culture solution runner, and the culture solution runner is communicated with the outside through a culture solution hole; the cell culture layer is provided with a plurality of stem cell culture chambers; each cell culture chamber is communicated with the outside through the hydrogel channel and the cell ball injection hole; the area of the porous membrane layer at least covers the bottoms of all the cell culture chambers; the metabolite collecting layer is provided with a plurality of collecting chambers, and at least one collecting chamber is arranged below each cell culture chamber; the vascularization organ chip is further provided with a plurality of liquid taking channels, and each collecting cavity is communicated with the outside through the corresponding liquid taking channel. The vascularized organ chip provided by the invention can realize in-situ collection and dynamic analysis of metabolites.

The invention discloses a novel oligopeptide PSP for angiogenesis and vascularized bone regeneration. The tripeptide innovatively screened by the invention is short in amino acid sequence, small in molecular weight, high in bioavailability, low in synthesis cost and easy to transport, modify and recombine. The tripeptide provided by the invention can significantly promote vascularized bone regeneration and improve skull defect repair. Good application prospects are realized.

Disclosed herein are spinal cord models including a cell scaffold including methacrylated hyaluronic acid (HAMA) and dopamine-modified hyaluronic acid (HA-Cat), and a spinal cord spheroid or organoid or a fragment thereof cultured on the cell scaffold. Said spinal cord spheroids or organoids or fragments thereof can be co-cultured with blood vessel spheroid organoids to form blood-spinal cord barrier models. Also disclosed herein are methods of making and using said models.

This invention belongs to the field of microfluidic chip technology and discloses a device and method for improving liver function in a microphysiological system. The device for improving liver function in a microphysiological system comprises a microfluidic chip, channels, a peristaltic pump, and a reservoir; the microfluidic chip includes a top plate layer, a fluid channel layer, a porous membrane, and a tissue culture chamber layer. The method for improving liver function in a microphysiological system includes adjusting the spatial arrangement of multiple tissues around the liver, the fluid flow rate of the microfluidic system, and liver vascularization. The method of this invention plays a crucial role in studying the interactions and crosstalk between different tissues, and enhances liver function by regulating the interaction between the liver and other tissues.

The invention discloses a lung tissue organoid culture method. The method comprises the following steps: firstly, carrying out three-dimensional differentiation culture on matrigel by utilizing lung basal cells in vitro to construct an organoid with alveolar epithelium characteristics; then, the organ is transplanted into the spleen of a recipient animal subjected to ectopic surgery (shifted to subcutaneous tissues). The rich blood vessel network and the unique microenvironment of the spleen greatly promote rapid vascularization, structural remodeling and functional maturation of organoids, and lung tissue highly simulating natural alveolus is formed. The invention overcomes the defects of insufficient vascularization and immature function of the existing organ-like model, provides an excellent in-vivo platform for researching lung development and disease mechanisms and carrying out drug screening and toxicological evaluation, and has important application value in the field of regenerative medicine.

The present invention relates to a method and device for long-term cryopreservation of a biological sample, including a vascularized tissue, innervated tissue, or both, such as but not limited to a limb.

The invention provides a multilayer artificial skin flap containing a built-in nutritional blood vessel and a preparation method of the multilayer artificial skin flap, and belongs to the technical field of tissue engineering and regenerative medicine. The artificial skin flap is sequentially provided with a first decellularized meat pulp matrix layer, a nutritional blood vessel, a fabric layer and a second decellularized meat pulp matrix layer from top to bottom. The nutrition blood vessel can construct a nutrition delivery channel for surrounding tissues, and promote rapid proliferation of cells and formation of a new blood vessel network; the decellularized meat pulp matrix layer provides a bionic microenvironment and supports cell adhesion, migration and growth; the fabric layer plays a role in enhancing mechanical support and simultaneously endows the flap with good stitchability and tear resistance; in addition, the nutritional blood vessel and the fabric layer are both made of degradable filaments, the degradation period can be regulated and controlled according to needs, and space and mechanical support smooth transition is vacated for tissue growth. The artificial skin flap has excellent biocompatibility, controllable degradability, active vascularization ability and proper mechanical properties, and is suitable for full-thickness skin defect repair and soft tissue regeneration engineering.

This invention discloses a multi-effect synergistic repair scaffold for large bone defects and its preparation method. This invention relates to the field of biomedical materials technology. The scaffold comprises a three-dimensional biomimetic structure with a central tubular channel. The structure consists of a core layer, a middle layer, and an outer layer from the inside out. The core layer is a hollow structure made of shape memory polymer material, and the middle layer is a biodegradable biomimetic porous ceramic matrix loaded with a first bioactive factor. The advantages of this invention are: by constructing a hierarchical structure of core layer-middle layer-outer layer, it integrates mechanical support, osteogenic activity, vascularization, and immune regulation functions, achieving synergistic intervention throughout the entire process of repairing large bone defects. The core layer uses a shape memory polymer, which not only provides axial compressive strength but also allows the scaffold to be implanted via minimally invasive surgery in a folded state. After implantation, it automatically recovers its shape at body temperature, closely adhering to the user's bone interface.

The utility model provides a full-porous spine fusion cage with an extremely-small curved surface structure, and belongs to the technical field of medical instruments. The utility model comprises an extremely small curved surface porous structure which is a three-period spiral curved surface; the bone grafting windows are arranged on the pair of cambered surfaces of the extremely-small-curved-surface full-porous structure and are used in cooperation with bone grafting; and the instrument clamping area is arranged in the middle of the bottom surface of the extremely-small-curved-surface porous structure, is communicated with the bone grafting window and is used for clamping the spinal fusion cage by an instrument. According to the utility model, an extremely small curved surface porous structure formed by a three-period spiral curved surface is adopted, the comprehensive mechanical property is excellent, and the static compression strength and the fatigue strength are improved by more than 50%; the matching degree of the elasticity modulus and the human skeleton is high, the stress load is uniformly distributed, the mechanical reliability is high, and the anti-fatigue capability is strong; no compact solid part exists, so that the contact area with bones and the bone tissue ingrowth space are increased; blood is rapidly sucked into the pore channel through the capillary effect, bone fusion and vascularization are facilitated, and bone conduction, bone fusion and bone ingrowth performance is outstanding.

The invention relates to the technical field of smoke toxicity testing, and discloses a cigarette mainstream smoke total particulate matter toxicity testing method based on a vascularized micro-fluidic chip. The vascularized micro-fluidic chip is constructed through the human lung adenocarcinoma cell layer, the human umbilical vein endothelial cell layer and the human liver cancer cell layer which are sequentially arranged, the chip is communicated with air through the lung adenocarcinoma cell layer, and a culture medium dynamically flows in the exposure process of total particulate matters in cigarette mainstream smoke; and substance exchange among human lung adenocarcinoma cells, human umbilical vein endothelial cells and human liver cancer cells is simulated based on a PC membrane and a dynamic circulating culture medium, so that the whole process of smoke in-vivo exposure can be simulated, and smoke toxicity can be accurately represented by testing key indexes of cytotoxicity.

The invention discloses a large-size organ vascularization culture device and a large-size organ vascularization culture method, belongs to the field of tissue engineering and micro-fluidic organ chips, and aims to overcome the defects of the large-size organ vascularization culture device in the aspects of structural closure, operation flexibility and the like. The culture device provided by the invention comprises an openable micro-fluidic chip module used for bearing a mixture of endothelial cells and matrigel and adding large-size organ-like tissues, and the openable micro-fluidic chip module is arranged in a cell culture box used for adjusting a culture environment and monitoring cultured organs. The liquid inlet end and the liquid outlet end of the openable micro-fluidic chip module extend out of the cell culture box through pipelines and communicate with the liquid path perfusion system, shearing force can be continuously optimized according to different growth stages of organ tissues, and synchronous maturation of blood vessels and organoids is guaranteed. The culture device and the culture method are mainly used for large-size organ vascularization tissue culture.