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Preparation method of biological scaffold material and SVF assistant adipose tissue
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A bio-scaffold, adipose tissue technology, applied in medical science, prosthesis, etc., can solve problems such as difficult survival, and achieve the effect of easy survival, promoting shape recovery, and less trauma.
Inactive Publication Date: 2015-10-21
广东佰鸿干细胞再生医学有限公司
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Problems solved by technology
However, breast augmentation needs to fill a large amount of graft material, and if the new cells do not have a good extracellular scaffold, it is difficult to survive
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example 1
[0034] Step 1. Purification of adipose tissue
[0035] 40ml of adipose tissue obtained by liposuction was centrifuged at 1200r / min at 21°C for 3min, and the liquid part was removed to obtain 20ml of solid fat particles. Then remove the connective tissue from the obtained fat particles, cut the large pieces of tissue with tissue scissors, and use a 1ml syringe to remove the needle and extract it normally;
[0036] Step 2, preparation of fat SVF
[0037] Take out 10ml of the above-mentioned purified fat particles, add 10ml of physiological saline to wash, centrifuge at 1200r / min, 21°C for 3min, remove the liquid part, and obtain solid fat particles. Then add collagenase solution prepared with 10ml normal saline (Shanghai Qiaoyuan Biopharmaceutical Co., Ltd.), 200U / ml, 37°C, digest at constant temperature and shake for 30min, collect the digestive juice, centrifuge at 800r / min, 21°C for 5min, remove the supernatant, take Add 10ml of sterile normal saline to the cell pellet, centr...
example 2
[0044] Step 1. Purification of adipose tissue
[0045] Centrifuge 50ml of adipose tissue obtained after abdominal injection of swelling solution from a person with facial nasolabial folds at 1200r / min at 21°C for 3min, remove the liquid part, and obtain 25ml of solid fat particles. Then remove the connective tissue from the obtained fat particles, cut the large pieces of tissue with tissue scissors, and use a 1ml syringe to remove the needle and extract it normally;
[0046] Step 2, preparation of fat SVF
[0047] Take out 12ml of the above-mentioned purified fat particles, add 12ml of physiological saline to wash, centrifuge at 1200r / min, 21°C for 3min, remove the liquid part, and obtain solid fat particles. Then add collagenase solution prepared with 12ml normal saline (Shanghai Qiaoyuan Biopharmaceutical Co., Ltd.), 200U / ml, 37°C, digest at constant temperature and shake for 30min, collect the digestive juice, centrifuge at 800r / min, 21°C for 5min, remove the supernatant, ...
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Abstract
The invention discloses a preparation method and an application of a biological scaffold material and SVF assistant adipose tissue. Under an aseptic condition, the biological scaffold material, a stromal vascular fraction (SVF) prepared by digesting purified adipose granules with a medical-graded collagenase, and the purified adipose particles are evenly mixed according to a certain proportion to prepare an adipose graft for filling. The SVF is a mixture of adipose mesenchymal stem cells and various cells, is transplanted after being mixed with autogenous adipose particles, and can be continued to be developed into a mature adipose tissuein vivo; the biological scaffold material can provide a support for transplanted adipose, allows the transplanted tissue to be prone to revascularization, and improves the survival rate of the transplanted tissue; the transplanted tissue is suitable for being injected with a 16 G or 18 G needle, facilitates clinical application, and can be used for repairing of concave deformity (such as tempora and cheek concave), beautifying packing of faces and chests, soft tissue function reconstruction and the like; and especially in the beautifying packing, the prepared transplanted adipose tissue can promote shape recovery and prevent liquefactive necrosis.
Description
technical field [0001] The invention belongs to the technical field of medical fat grafting, and in particular relates to a preparation method and application of a biological scaffold material and an SVF-assisted fat grafting material. Background technique [0002] The data shows that the ages of women undergoing breast augmentation in plastic surgery hospitals are concentrated between 25 and 45 years old, among which more than 67.58% choose autologous fat for breast augmentation; Between the ages, more than 45% of people choose autologous fat to remove wrinkles and anti-aging cosmetics. [0003] Injection-type autologous fat particle transplantation is a fat transplantation scheme frequently used in clinical practice, and most of the grafts come from the extraction of autologous fat. The advantages of this method are good biocompatibility, abundant tissue sources, easy shaping, and no adverse reactions such as immune rejection; the disadvantage is that the fat particles in...
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