85 results about "Transplanted tissue" patented technology

Cell-based compositions and methods of their use to inhibit an adverse immune response such as graft versus host disease or rejection of transplanted tissue in a transplant recipient that is histocompatibility mismatched to the transplant donor are disclosed. The compositions and methods utilize postpartum-derived cells, such as cells derived from the placenta or umbilicus.

A process of mechanical separation of embryos from seeds for genetic transplantation employs counter-rotating cylinders together with one or more culling, hydration, separation, and viability testing steps to provide high-throughput of viable, transplantable tissue.

The present invention provides a therapeutic method for treating biological diseases that includes the administration of an effective amount of a suitable antibiotic agent, antifungal agent or antiviral agent in conjunction with an A2A adenosine receptor agonist. If no anti-pathogenic agent is known the A2A agonist can be used alone to reduce inflammation, as may occur during infection with antibiotic resistant bacteria, or certain viruses such as those that cause SARS or Ebola. Optionally, the method includes administration of a type IV PDE inhibitor.

A method for stimulating perfusion and minimizing postischemic reperfusion injury. A patient is selected having compromised perfusion in a tissue or in the microcirculation of an organ. An ultrasound transducer is applied to a location near the tissue. The transducer is activated to initiate exposure of the tissue to ultrasound at a frequency of 100 KHz to 2.5 MHz for 0.5 to 15 minutes duration, wherein local vasodilatation is stimulated. In certain cases, the methods further include a step of confirming that local vasodilatation is stimulated by measuring enhancement in perfusion. Ultrasound devices are also described for carrying out the methods. The devices and methods can be used to enhance myocardial perfusion, cerebral perfusion, and perfusion of transplanted tissues.

A method for inhibiting rejection by a recipient animal of a transplanted tissue, said method comprising modifying, eliminating, or masking an antigen which, when present on the surface of a cell of said tissue, is capable of causing a T-lymphocyte-mediated response in said animal, to inhibit antigen-mediated interaction between said cell and a T-lymphocyte of said animal without causing lysis of said cell.

The present invention provides a therapeutic method for treating biological diseases that includes the administration of an effective amount of a suitable antibiotic agent, antifungal agent or antiviral agent in conjunction with an A2A adenosine receptor agonist. If no anti-pathogenic agent is known the A2A agonist can be used alone to reduce inflammation, as may occur during infection with antibiotic resistant bacteria, or certain viruses such as those that cause SARS or Ebola. Optionally, the method includes administration of a type IV PDE inhibitor.

Immunomodulating agents comprising at least one Fc receptor ligand and at least one immunosuppressive factor are provided as are methods for their manufacture and use. The immunomodulating agents may be in the form of polypeptides or chimeric antibodies and preferably incorporate an immunosuppressive factor comprising a T cell receptor antagonist. The compounds and compositions of the invention may be used to selectively suppress the immune system to treat symptoms associated with immune disorders such as allergies, transplanted tissue rejection and autoimmune disorders including lupus, rheumatoid arthritis and multiple sclerosis.

The present invention generally relates to methods, compositions and uses thereof for enhancing vascularization of a tissue or cell transplant for transplantation into a subject. In particular, one aspect of the present invention provides methods and compositions comprising the use of a population of stromal vascular fraction (SVF) cells to encapsulate or surround a tissue or cell transplant to enhance vascularization of the tissue or cell transplant. Another aspect of the present invention provides methods and compositions for enhancing vascularization of a tissue or cell transplant by combining a population of SVF cells with a tissue or cell transplant to form a transplant mixed with SVF cells. Another aspect provides a composition comprising an engineered corpus cavernosum tissue comprising SVF cells and corpus cavernosum cells, wherein the SVF cells can be mixed with or encasing the corpus cavernosum cells, and methods of uses thereof, for example in method for the treatment of impotence and erectile dysfunction and / or enhance or construct a penis. In some embodiments, the SVF cells can be generically engineered to secrete therapeutic proteins or pro-angiogenic factors.

Immunomodulating agents comprising at least one Fc receptor ligand and at least one immunosuppressive factor are provided as are methods for their manufacture and use. The immunomodulating agents may be in the form of polypeptides or chimeric antibodies and preferably incorporate an immunosuppressive factor comprising a T cell receptor antagonist. The compounds and compositions of the invention may be used to selectively suppress the immune system to treat symptoms associated with immune disorders such as allergies, transplanted tissue rejection and autoimmune disorders including lupus, rheumatoid arthritis and multiple sclerosis.

Immunomodulating agents comprising at least one Fc receptor ligand and at least one immunosuppressive factor are provided as are methods for their manufacture and use. The immunomodulating agents may be in the form of polypeptides or chimeric antibodies and preferably incorporate an immunosuppressive factor comprising a T cell receptor antagonist. The compounds and compositions of the, invention may be used to selectively suppress the immune system to treat symptoms associated with immune disorders such as allergies, transplanted tissue rejection and autoimmune disorders including lupus, rheumatoid arthritis and multiple sclerosis.

The present invention relates to compositions that reduce the extent of cellular and tissue damage in response to injury or disease. Methods of using the compositions to repair reperfusion injury, and structural tissue damage due to trauma, disease or aging are provided. Also provided are methods of using the compositions to reduce damage to transplanted tissue and organs. The compositions may also be used in oncology applications.

A percutaneously implantable device for the treatment of a cardiac condition or other disease is disclosed herein, the device capable of delivery and maintenance of a therapeutic scaffold. A therapeutic scaffold may comprise viable tissue to impart or restore normal cardiac function, or other therapeutic agent for the treatment of disease or injury. Viable tissue may comprise a pacemaker gene or other genes intended to impart a pacemaker function to either host tissue or transplanted tissue, or both. Further, a device according to the invention may be used for the implantation and maintenance of viable tissue to induce or enhance muscle contraction of a subject for the treatment of a disease or disorder.

The invention relates to the technical field of agriculture plantation, in particular to a planting method for Dendrobium officinale Kimura et Migo. The method includes the steps of seedbed preparation, culture matrix preparation, flowerpot preparation, field planting, watering, fertilizer application, pest control, temperature and humidity control and the like. The method has the advantages that the survival rate of transplanted tissue culture seedlings is high and can reach 99% or above; biogas slurry is applied and sprayed, especially biogas slurry generated by a soft biogas digester is used for specially processing a culture matrix, produced Dendrobium officinale Kimura et Migo has plump leaves and stems, the content of various nutrition ingredients is high, growth is rapid, yield is high, the effective medicinal component content is higher than that of wild Dendrobium officinale Kimura et Migo, the pest and disease damage occurrence rate is obviously decreased, cost is reduced, and the food safety of Dendrobium officinale Kimura et Migo is improved. Dendrobium officinale Kimura et Migo can be harvested in 14-16 months after being planted through the method.

The present invention generally relates to methods, compositions and uses thereof for enhancing vascularization of a tissue or cell transplant for transplantation into a subject. In particular, one aspect of the present invention provides methods and compositions comprising the use of a population of stromal vascular fraction (SVF) cells to encapsulate or surround a tissue or cell transplant to enhance vascularization of the tissue or cell transplant. Another aspect of the present invention provides methods and compositions for enhancing vascularization of a tissue or cell transplant by combining a population of SVF cells with a tissue or cell transplant to form a transplant mixed with SVF cells. In some embodiments, the SVF cells can be on the surface or embedded within a three-dimensional matrix. In some embodiments, the SVF cells can be generically engineered to secrete therapeutic proteins or pro-angiogenic factors.

A tissue equivalent for transplantation having a three-dimensional structure which is cultured in vitro, contains cells to be transplanted and which can be transplanted into a living body after the culture, characterized by including a scaffold layer mainly culturing a scaffold constituting the three-dimensional structure and a cell layer which is localized at least in a part of the surface of the tissue equivalent for transplantation continuously with the scaffold layer and which contains the cells to be transplanted or extra cellular matrix in a larger amount than the scaffold layer. This tissue equivalent is appropriately employed as a tissue equivalent for transplantation in a relatively large size. This tissue equivalent enables realization of prompt fixation to the neighborhood of the transplanted tissue and prevention of falling off.

The present invention, at least in part, relates to the discovery of efficacious delivery of an RNAi agent (in preferred aspects of the invention, an siRNA) to a transplantable tissue. Organ rejection, transplantation-mediated transmission of viral infection, and triggering of apoptosis in transplanted tissues can each be minimized by the methods and compositions of the instant invention. The RNAi agent(s) of the instant invention can be delivered as “naked” molecules, or using liposomal and other modes of delivery, to transplantable tissues. Such delivery can occur via perfusion of the RNAi agent in solution through the vasculature of a whole or partial organ; or tissues including transplantable cells and cell lines may be bathed, injected or otherwise treated with RNAi agents. Preferred transplantable tissues include, for example, pancreas, liver, kidney, heart, lung, and all cells and cell lines derived from such tissues (e.g., pancreatic islet cells that may, e.g., be transplanted as a treated population).

The invention relates to a clonal tissue culture breeding method of Liquidambar formosana Hance, belonging to the technical field of tree tissue culture breeding. The method comprises the following steps of: sterilization of explant materials, induction of sprouts, multiplication of sprouts, rooting induction, hardening of rooted seedlings, and transplanting and management of tissue culture seedlings. Specifically, the method comprises the steps of: removing leaves of a one-year-old shoot, cutting the shoot into sections with 1-2 axillary buds respectively, sterilizing the sections, conducting induced culture to the sections by using induced culture media, conducting multiplication culture and rooting culture to new sprouts, hardening and domesticating the cultured rooted seedlings, finally transplanting the domesticated seedlings on sterilized media, and managing the transplanted seedlings. By adopting the method, the breeding is not limited by seasons, the production cost is low and the land resources are saved; and the situation of browning can be effectively avoided, the effective multiplication rate is high, the rooted seedlings grow tidily, the culture period is short, the survival rate of the transplanted tissue culture seedlings is high, the seedling cultivation period is short, the clonal breeding of the Liquidambar formosana Hance can be pushed forward and the method plays an important role in large-scale propagation and popularization of improved varieties.

The invention relates to the technical field of agricultural planting, particularly a planting method for dendrobium officinale. The method comprises the steps of: preparing a seedbed; preparing a cultivation matrix; preparing flower pots; performing fixed planting; performing watering; performing fertilization; performing pest control; controlling temperature and humidity and the like. The planting method provided by the invention has the beneficial effects that the survival rate of transplanted tissue culture seedlings reaches over 99%; by virtue of spraying biogas slurry, particular biogas slurry produced in a soft biogas pool, performing special treatment on a cultivation matrix, fermenting silkworm feces as top dressing and self-making a natural plant bactericide to prevent and treat plant diseases and insect pests, the produced dendrobium officinale is fleshy in leaf and stem, high in various nutritional components, quick to grow, high in yield and higher than wild dendrobium officinale in effective medicinal component content, and the occurrence rate of plant diseases and insect pests is obviously reduced, so that the cost is lowered, and the food safety of dendrobium officinale is improved. By adopting the method to plant dendrobium officinale, harvest within 12-14 months can be realized.

The present invention provides methods and compositions for converting non-Tregs into Tregs. The converted Tregs are referred to as inducible Tregs (iTregs). The iTregs are useful for preventing, suppressing, blocking or inhibiting an immune response. For example the iTregs are useful for preventing rejection of a transplanted tissue in a human or other animal host, or protecting against graft vs host disease. The iTregs can also be used to treat autoimmune diseases.

The invention relates to the technical field of agricultural planting, particularly a planting method for dendrobium officinale. The method comprises the steps of: preparing a seedbed; preparing a cultivation matrix; preparing flower pots; performing fixed planting; performing watering; performing fertilization; performing pest control; controlling temperature and humidity and the like. The planting method provided by the invention has the beneficial effects that the survival rate of transplanted tissue culture seedlings reaches over 99%; by virtue of spraying biogas slurry, particular biogas slurry produced in a soft biogas pool, fermenting silkworm feces as top dressing and self-making a natural plant bactericide to prevent and treat plant diseases and insect pests, the produced dendrobium officinale is fleshy in leaf and stem, high in various nutritional components, quick to grow, high in yield and higher than wild dendrobium officinale in effective medicinal component content, and the occurrence rate of plant diseases and insect pests is obviously reduced, so that the cost is lowered, and the food safety of dendrobium officinale is improved. By adopting the method to plant dendrobium officinale, harvest within 14-16 months can be realized.

The present invention provides a novel approach to the modulation of the immune response, directing it towards specific antigens, away from antigens against which no response is desired. The invention is based on the use of viral immune evasion proteins, such as UL49.5, which block antigen presentation to CD8+ T cells. The viral immune evasion proteins are used for: 1) the induction of tumor-specific or virus-specific immunity in cases where a conventional immune response is absent due to antigen processing defects; 2) the induction of empty MHC class I molecules at the cell surface that can be loaded with peptides of a desired specificity; 3) the inhibition of unwanted immune responses against transplanted tissues or organs, e.g. against islets of Langerhans in type 1 diabetes or allogeneic stem cells, or against self antigens in the case of autoimmunity.

The invention provides a degradable seedling raising holed tray.The degradable seedling raising holed tray is prepared from deinked pulp, plant fiber and an adhesive, wherein the mass ratio of the deinked pulp to the plant fiber to the adhesive is 60-80 to 20-40 to 0-2, and the plant fiber is selected from one or more of straw, bamboo reeds, reeds and wheat straw.The invention further provides a preparation method of the degradable seedling raising holed tray.Firstly, plant fiber raw materials are dried and then are smashed to reach 40-70 mesh; the deinked pulp, the plant fiber and the adhesive are mixed according to the mass ratio, are evenly stirred and are charged into a mold, pressing, molding and mold release are performed, and then drying is performed to reach 10-20% of water content, namely the finished product degradable seedling raising holed tray is obtained.The degradable seedling raising holed tray has a certain saline-alkaline resisting and decomposing role, is isolated from the outside at a seedling adapting stage and also has degradability, root systems can grow to penetrate through the holed tray after seedlings adapt to a salt marsh environment, and finally complete degradation is achieved.The degradable seedling raising holed tray improves the survival rate of transplanted tissue cultured seedlings and reduces the transplanting cost.

The invention discloses a preparation method and an application of a biological scaffold material and SVF assistant adipose tissue. Under an aseptic condition, the biological scaffold material, a stromal vascular fraction (SVF) prepared by digesting purified adipose granules with a medical-graded collagenase, and the purified adipose particles are evenly mixed according to a certain proportion to prepare an adipose graft for filling. The SVF is a mixture of adipose mesenchymal stem cells and various cells, is transplanted after being mixed with autogenous adipose particles, and can be continued to be developed into a mature adipose tissue in vivo; the biological scaffold material can provide a support for transplanted adipose, allows the transplanted tissue to be prone to revascularization, and improves the survival rate of the transplanted tissue; the transplanted tissue is suitable for being injected with a 16 G or 18 G needle, facilitates clinical application, and can be used for repairing of concave deformity (such as tempora and cheek concave), beautifying packing of faces and chests, soft tissue function reconstruction and the like; and especially in the beautifying packing, the prepared transplanted adipose tissue can promote shape recovery and prevent liquefactive necrosis.

The invention relates to a method for cultivating thornless Lycium ruthenicum Murr.. The cultivation method is characterized by comprising the following steps: by using the stem or mature seed of thorny Lycium ruthenicum Murr. as the original donor material, performing culture by using the stem or leaf as an explant to obtain a sterile tissue culture seedling; inoculating the obtained tissue culture seedling into an improved 1 / 4 or 1 / 2 MS solid culture medium, and performing strong seedling culture under the sunlight; after the sound seedling culture, performing transplanting and acclimatization; putting the newly transplanted tissue culture seedling under scattered light, and periodically pouring distilled water to keep the water content of the culture medium at the field moisture capacity of 100%; performing water control treatment on the healthy potted tissue culture seedling which survives after the transplanting and acclimatization, and periodically pouring a 1 / 8 improved MS nutrient solution or 1 / 4 improved MS nutrient solution; and putting the potted seedling under the sunlight, and keeping the ambient temperature at 18-28 DEG C. The thorny Lycium ruthenicum Murr. is utilized as the starting material to successfully cultivate abundant thornless Lycium ruthenicum Murr. plants, and the thornless plants can grow and develop very well, thereby laying the foundation for the cultivation of new species of thornless Lycium ruthenicum Murr..

The present invention provides compositions, methods, and assays for treating an inflammatory and / or autoimmune disease, and / or transplanted tissue rejection using anti-alphabeta TCR antibodies and antibody fragments. Anti-alphabeta TCR antibodies are antibodies which bind to a alphabeta TCR. Anti-alphabeta TCR antibodies produced by the hybridoma TOL101 MCB are also provided. Methods for treatment of an inflammatory disease, an autoimmune disease and for tissue transplant rejection using therapeutic dosing regimen of anti-alphabeta TCR antibodies and antibody fragments and for upregulating the numbers of Treg T-cells are also provided.

The invention discloses a fast and efficient agrobacterium-mediated rice stem tip genetic transformation method. The method comprises the following major steps: directly soaking mature rice seeds in tap water, accelerating germination, then transversely cutting the coleoptiles to expose stem tips as transformation receptors, performing genetic transformation by virtue of agrobacterium mediation without sterilization treatment, putting the transformed materials under an appropriate environment to allow plants to grow, and carrying out transgenic screening and identification to obtain the transgenic rice plants. According to the fast and efficient agrobacterium-mediated rice stem tip genetic transformation method, callus induction and a series of subsequent tissue culture processes are avoided, the defects of materials affected by bacteria, difficult regeneration, vitrification, browning, low survival rate of transplanted tissue cultured seedlings and the like in the tissue culture process are avoided, the rice genetic transformation cost is reduced, the restriction of the rice genotype to the transformation efficiency is effectively overcome, and a large quantity of transgenic rice plants can be obtained in short time; the fast and efficient agrobacterium-mediated rice stem tip genetic transformation method has a significant value in good-quality rice culture and rice genetic transformation research.

The invention relates to the use of cells from cell lines, which are derived from early embryonic stages, for the donor-specific increase in transplantation tolerance and for repairing damaged tissue. Areas of application of the invention include the field of medicine and the pharmaceutical industry. The aim of the invention is to produce a donor-specific immunotolerance in order to prevent a rejection of the transplanted tissue due to an immune response and thus to be able to limit the administration of immune suppressive agents. In order to produce a donor-specific immunotolerance, embryonic stem cell-like cell lines (ECL) are obtained from blastocysts and are transfected with genetic material of the donor, which codes for the MHC haplotypes. The cells produced in such a manner are administered to the recipient before the transplantation for producing an immunotolerance against the tissue to be transplanted or for regenerating already damaged tissue.

The present invention generally relates to methods, compositions and uses thereof for enhancing vascularization of a tissue or cell transplant for transplantation into a subject. In particular, one aspect of the present invention provides methods and compositions comprising the use of a population of stromal vascular fraction (SVF) cells to encapsulate or surround a tissue or cell transplant to enhance vascularization of the tissue or cell transplant. Another aspect of the present invention provides methods and compositions for enhancing vascularization of a tissue or cell transplant by combining a population of SVF cells with a tissue or cell transplant to form a transplant mixed with SVF cells. In some embodiments, the SVF cells can be on the surface or embedded within a three-dimensional matrix. In some embodiments, the SVF cells can be generically engineered to secrete therapeutic proteins or pro-angiogenic factors.