4results about How to "Increased activation" patented technology

The present invention relates to a specific binding protein targeting PD-L1 and CD73, wherein the specific binding protein is capable of specifically binding to PD-L1 and / or a fragment thereof, and CD73 and / or a fragment thereof. The present invention further relates to use of the specific binding protein in the treatment, prevention and / or diagnosis of diseases such as immune diseases, acute and chronic inflammatory diseases, and tumor diseases.

The invention discloses a culture method of DC-CTL cells, and particularly relates to the technical field of biotechnology and medicine. The invention provides a novel DC-CTL cell culture method based on iPSC, aiming at the technical problems that a traditional DC-CTL cell culture method is limited in cell source, greatly influenced by individual difference, poor in stability and repeatability, insufficient in cell activity and killing ability and the like. A self-made additive and a specific cell factor are combined, SOCS1-siRNA is transfected through a liposome transfection method, the SOCS1 gene is effectively silenced, and the T cell activity is enhanced. According to the method, the proportion of CD3 + CD8 + T cells in the DC-CTL cells reaches 85% or above, the content of IFN-gamma is larger than or equal to 500 pg / mL, the content of TNF-alpha is larger than or equal to 300 pg / mL, and the killing rates under the effect target ratios of 10: 1, 20: 1 and 40: 1 reach 57%, 63% and 72% respectively.

The invention provides methods and compositions for treating locally advanced or metastatic breast cancer and for enhancing immune function in an individual having locally advanced or metastatic breast cancer. The methods comprise administering a PD-1 axis binding antagonist and a taxane.

The utility model discloses an automatic stirrer for activating saccharomyces cerevisiae, which comprises a supporting seat, an anti-caking component, a mixing component, a temperature sensor, a tank body, a heat insulation cover, a discharge valve and a snake-shaped electric heating wire. Active dry yeast is poured into the discharging hopper, enters the rotary joint and the round pipe through the discharging hopper, is discharged into the screening barrel through a group of through grooves, enters the tank body through the screening holes, is stirred by the mixing assembly through warm water, and simultaneously passes through the anti-caking assembly, so that the active dry yeast is discharged into the tank body through the screening holes. The group of scattering rods are used for scattering the active dry yeast which is in contact with warm water and is caked in the screening barrel, so that the agglomeration phenomenon of the active dry yeast is avoided, and the scattered active dry yeast gradually passes through the screening holes to be in contact with the warm water in the tank body, so that the active dry yeast is quickly and fully dissolved in the warm water; and the proper temperature can improve the activation degree, the activation quality and the activation efficiency of the active dry yeast.