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Method for cultivating thornless Lycium ruthenicum Murr.

A technology of black fruit wolfberry and seeds is applied in the field of cultivating thornless plants of black fruit wolfberry, which can solve the problems of reducing the economic benefit of plantation, increasing the difficulty of afforestation and daily management, and achieving the effect of good growth and development.

Active Publication Date: 2017-10-03
SHENYANG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But the hard and firm thorns of Lycium barbarum not only increase the difficulty of afforestation and daily management (especially fruit picking), but also reduce the economic benefits of plantation

Method used

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  • Method for cultivating thornless Lycium ruthenicum Murr.
  • Method for cultivating thornless Lycium ruthenicum Murr.

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] 1. Cultivate Lycium barbarum tissue culture seedlings

[0022] According to the method of the patent (201610206401.1), cut the healthy young leaves of sterile seedlings or regenerated plants into small pieces with a diameter of 5mm, and connect them horizontally with 6-BA 0.1mg / L+NAA 0.1mg / L+4.5g / L agar + 40g / L sucrose MS solid medium. They were cultured under weak scattered light at 25°C for 2 weeks, and then transferred to a 14W led and other light for 8 hours of light every day. The pH value of the above MS solid medium was adjusted to 5.4 with 1mol / L KOH solution before autoclaving at 121°C for 15 minutes. The explants were subcultured 4 weeks after inoculation, and the culture material was transferred to the same medium. At this time, a large number of bud clusters appeared on the surface of callus formed by most leaf explants. When the bud grows to a height of more than 3 cm, it is cut off, and inserted into 1 / 2MS solid medium with a pH value of 5.4 without ad...

Embodiment 2

[0031] 1. Cultivate Lycium barbarum tissue culture seedlings

[0032]According to the method of the patent (201610206401.1), the stem section of the sterile seedling or the stem section of the regenerated plant is used as the explant for tissue culture. Cut the sterile tender stems without leaves into 0.5-2cm segments, and insert the lower end down into MS solid culture with a pH value of 5.6 containing 6-BA 0.2mg / L+5.0g / L agar+35g / L sucrose basically. 10h of light per day at 24°C. At 4 weeks after inoculation, the material was subcultured to the same medium for culture. When the leaf axil cluster buds or callus adventitious buds produced by the culture material reached a height of 4 cm, they were cut and inserted into 1 / 2MS solid culture without adding any plant growth regulator to induce rooting.

[0033] 2. Strong seedling cultivation

[0034] Rooted plantlets were inoculated into modified 1 / 2 MS solid medium in glass bottles. Modified 1 / 2MS solid medium is in addition...

Embodiment 3

[0040] 1. Cultivate Lycium barbarum tissue culture seedlings

[0041] The fungus thorny wolfberry stems in the greenhouse were collected. Soak the stem section with 75% (volume / volume) alcohol on a sterile ultra-clean workbench for 30 seconds, then soak it with 0.12% (mass / volume) mercuric chloride aqueous solution for 3 minutes, and rinse it with sterile water 4 times. Inoculate 2cm-long axillary stem segments with leaves on 1 / 2MS solid medium supplemented with 6-BA 0.3mg / L, and cultivate under light at 23°C for 12 hours a day. After the axillary buds of the stem section to be inoculated germinated shoots, the leaves and stem sections of the shoots were collected as explants. Referring to the method of patent 201610206401.1, the explants of tender stems were inoculated into MS solid medium containing 6-BA 0.05mg / L+NAA 0.05mg / L to induce callus and bud clusters; the leaf explants were inoculated into 6-BA 0.4mg / L+NAA 0.05mg / L MS solid medium to induce callus and bud cluster...

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Abstract

The invention relates to a method for cultivating thornless Lycium ruthenicum Murr.. The cultivation method is characterized by comprising the following steps: by using the stem or mature seed of thorny Lycium ruthenicum Murr. as the original donor material, performing culture by using the stem or leaf as an explant to obtain a sterile tissue culture seedling; inoculating the obtained tissue culture seedling into an improved 1 / 4 or 1 / 2 MS solid culture medium, and performing strong seedling culture under the sunlight; after the sound seedling culture, performing transplanting and acclimatization; putting the newly transplanted tissue culture seedling under scattered light, and periodically pouring distilled water to keep the water content of the culture medium at the field moisture capacity of 100%; performing water control treatment on the healthy potted tissue culture seedling which survives after the transplanting and acclimatization, and periodically pouring a 1 / 8 improved MS nutrient solution or 1 / 4 improved MS nutrient solution; and putting the potted seedling under the sunlight, and keeping the ambient temperature at 18-28 DEG C. The thorny Lycium ruthenicum Murr. is utilized as the starting material to successfully cultivate abundant thornless Lycium ruthenicum Murr. plants, and the thornless plants can grow and develop very well, thereby laying the foundation for the cultivation of new species of thornless Lycium ruthenicum Murr..

Description

technical field [0001] The invention belongs to a plant cultivation method, in particular to the cultivation of a thornless plant of wolfberry black fruit. Background technique [0002] Lycium ruthenicum Murr. is a perennial thorny shrub of the genus Lycium ruthenicum in the family Solanaceae. Plant thorns are divided into stem thorns (branch thorns), leaf thorns, skin thorns, thorns, etc. The thorns of wolfberry black fruit are stem thorns. It develops from the metamorphosis of axillary buds and is a metamorphosis of branches. Stem thorns are also called branch thorns, which are connected with the vascular bundles of the stem, and are hard and difficult to break and peel off. Wild black lycium barbarum often grows in various saline soils or desert environments such as saline-alkali wasteland, salinized sandy land, the shore of salt lakes, and roadsides in the form of shrubs. It has the functions of windbreak, sand fixation and soil quality improvement. Moreover, the hea...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 王钦美张志宏张桐欣刘俞帆吴月亮朱明周永斌
Owner SHENYANG AGRI UNIV
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