The invention discloses a preparation method of a 
Crassostrea hongkongensis fixation medium. The preparation method comprises the following steps that (1) a plurality of rectangular plastic sheets are made and a round hole with the 
radius being 3-5 mm is formed in each plastic sheet, wherein the length of the plastic sheets is 10-20 cm, the width of the plastic sheets is 2-5 cm and the thickness of the plastic sheets is 1-3 cm; (2) the plastic sheets are strung through a 
rope, a plastic tube is arranged between every two adjacent plastic sheets, the plastic tubes are arranged on the 
rope in a sleeved mode, and then the preparation process of the 
Crassostrea hongkongensis fixation medium is completed. The invention further discloses the 
Crassostrea hongkongensis fixation medium and an application method of the 
Crassostrea hongkongensis fixation medium. After being processed through 
oxalic acid, 
fresh water and the like, the 
Crassostrea hongkongensis fixation medium is used as an eyespot 
larva collection fixation medium; the 
Crassostrea hongkongensis fixation medium increases the 
metamorphosis rate of Crassostrea hongkongensis larvas, accelerates 
juvenile mollusk growth, improves the 
juvenile mollusk survival ability and increases the 
juvenile mollusk yield, has the advantages of being capable of being recycled multiple times, light, convenient to use, good in 
corrosion resistance and the like and is worthy of popularization and application.