18 results about "Metamorphosis" patented technology

The invention discloses a composite inducer for promoting adhesion and metamorphosis of marine bivalve mollusk larvae and an application method of the composite inducer. The composite inducer for promoting adhesion and metamorphosis of the marine bivalve mollusk larvae comprises glutamine, glutamic acid, potassium chloride, calcium chloride and taurine, and the concentration ratio of the glutamine to the glutamic acid to the potassium chloride to the calcium chloride to the taurine is 1: 1: 2 * 10: 1.7 * 10: 4. According to the application, glutamine, potassium ions, calcium ions, glutamic acid and taurine are matched, the application effect of the glutamine, the potassium ions, the calcium ions, the glutamic acid and the taurine in the aspect of improving the attachment and metamorphosis rate of the shellfish larvae is defined, the technical blank of multi-component cooperative regulation and control of the attachment and metamorphosis of the shellfish at present is filled, a new solution is provided for bivalve shellfish fry production, and the application prospect is wide. The method is of great significance in promoting healthy development of the mariculture industry.

The application discloses a construction method and application of an ovary cell line of Quasipaa spinosa. The method comprises the following steps: selecting metamorphosis period larva ovary tissue, disinfecting and obtaining the ovary tissue, and then using collagenase I digestion solution to digest the ovary tissue at 26 DEG C for 1 hour; after the tissue block adheres to the wall, using a low-osmotic-pressure complete culture medium to carry out primary culture at 25-27 DEG C, wherein the culture medium is 60% diluted DMEM / F12 culture medium containing 10%-15% fetal bovine serum; when the cell confluence degree is higher than 70%, using trypsin-EDTA to digest the cells for 1-2 minutes to subculture, and the subculture culture medium contains 30% cell conditional culture medium; and finally, the cells are cryopreserved through programmed cooling. The ovary epithelial cell line of Quasipaa spinosa is successfully established for the first time, the survival rate of the cells after recovery is high, and the karyotype is stable. The constructed cell line can be used for amphibian reproductive biology research, compound screening and in-vitro conservation of germplasm resources.

The application discloses a kind of Phascolosoma esculenta seedling cultivation methods, it is related to the technical field of mariculture.(1) parent culture;(2) labor induction;(3) sea ball larva cultivation;(4) the preparation of benthic cultivation pool;(5) planktonic larva metamorphosis induction;(6) the cultivation management of benthic larva.The application can effectively improve the efficiency of feeding sea mud of creeping stage larva, improve the dissolved oxygen in silt substrate, reduce the low dissolved oxygen caused by accumulated water during the process of dry, and the high mortality rate.

The invention discloses an artificial maturity promoting and fry breeding method for Peltella corii, which comprises the steps of cooling temporary rearing, maturity promoting, mating, spawning, hatching, high-density larva breeding, metamorphosis and the like, male snails and female snails are separately bred during maturity promoting, and a breeding scheme is optimized. According to the technical scheme, batch gonad maturation of parent snails according to plans is achieved, and the maturity is good; high-density breeding technologies such as fertilized egg hatching and larva breeding are overcome, a stable and efficient fry production process is established, a large number of high-quality, healthy and regular-specification young snails are provided for the breeding industry, and the provenance basis of the industry is fundamentally guaranteed.

The present invention provides a method for producing artificial seeds of whelks that enables year-round production by hatching larvae from egg masses obtained through spawning and implementing appropriate management according to larval development stages, thereby reducing mortality rates and increasing production yields. By managing hatched larvae according to early, middle, and late stages, it is possible to reduce mass mortality occurring during the artificial seed production process. Furthermore, by analyzing the correlation between the installation of chillers and the replacement cycle of housing filters for water quality control and water purification efficiency, it is possible to prevent the influx of external pathogens (ciliates, scuuchica) caused by rapid environmental changes during periods of high water temperature. Additionally, by artificially inducing larval settlement, the metamorphosis period can be shortened and growth variations between individuals can be reduced. Moreover, it is possible to establish a complete whelk aquaculture system that progresses from seed production to intermediate rearing to main rearing.

Pest and metamorphosis stage detection instrument for agricultural use

The invention discloses a method for promoting attachment of oyster larvae by using sound waves with specific frequency. According to the method, sound waves or vibration waves with the basic frequency located at the low frequency band are applied to culture water containing the oyster larvae, so that the larvae are attached and metamorphosed on an attachment substrate, and preferably, the oyster larvae are the oyster larvae in the attachment induction period. According to the invention, under the condition of being close to natural reef sound intensity, the condition that specific low-frequency (preferably about 20 Hz) sound waves have a remarkable and repeatable promoting effect on attachment of oyster larvae is found through system screening for the first time, and medium-high-frequency sound, especially high frequency above kilohertz, has a tendency of inhibiting and even damaging the attachment of the larvae is revealed; and a new technical path is provided for regulating and controlling the behaviors of the bivalve larvae by using an acoustic means.

This invention relates to the field of artificial breeding technology, specifically to a feed additive for the fully artificially bred Northeast Forest Frog and its application. The feed additive includes dandelion and Bacillus licheniformis, wherein the mass ratio of dandelion to Bacillus licheniformis is (0.02~0.1):1. The feed additive provided by this invention can effectively control digestive diseases in forest frog tadpoles, improve intestinal flora, promote weight gain and digestion in forest frog tadpoles, significantly improve the metamorphosis effect of forest frog tadpoles, and increase the survival rate of metamorphosed forest frog larvae.

The invention discloses a construction method and application of an ovarian cell line of hapalogenys nitens. The method comprises the following steps: selecting a metamorphosis stage larva ovarian tissue, disinfecting and obtaining, and digesting for 1 hour at 26 DEG C by using collagenase I digestive juice; after the tissue block adheres to the wall, a low-osmotic-pressure complete medium is used for primary culture at the temperature of 25-27 DEG C, and the medium is a 60% diluted DMEM / F12 medium containing 10%-15% of fetal calf serum; after the cell confluence degree exceeds 70%, trypsin-EDTA (Ethylene Diamine Tetraacetic Acid) is used for digesting for 1-2 minutes for passage, and a passage culture medium contains 30% of a cell condition culture medium; and finally performing programmed cooling and cryopreservation. According to the method, the ovarian epithelial cell line of the hapalogenys nitens is successfully established for the first time, the cell recovery survival rate is high, and the karyotype is stable. The constructed cell line can be used for amphibian reproductive biology research, compound screening and germplasm resource in-vitro preservation.

The invention relates to the technical field of aquaculture, in particular to a rana nigromaculata early breeding and froglet breeding method and system. On the basis of temperature control and water body stimulation of'fullness, drying and refullness', awakening and delivery are promoted under the condition of no exogenous hormone; a stepped terrain is constructed in the same temperature control greenhouse, the water level and the water-land ratio are adjusted in a linkage mode according to a population metamorphosis rate threshold value, and continuous operation of incubation, cultivation, metamorphosis and food domestication is achieved in cooperation with a water inlet and outlet net bag with the replaceable mesh number, an anti-escape fence and moving / film covering food tables on the two sides. Compared with a conventional pond dividing mode, breeding can be advanced by more than or equal to 2 months, and froglets appear on the market to July; the survival rate of incubation and metamorphosis is improved, drowning is remarkably reduced, the success rate of domestication and the specification uniformity are improved, meanwhile, carrying stress is reduced, and the unit fry cost is reduced.

The invention relates to the technical field of aquaculture, and discloses an industrial aquaculture workshop shell shrimp larva metamorphosis development cultivation method, which comprises the following steps: S1, when zoeae enters a metamorphosis development critical window, carrying out hypotonic period stress pretreatment on an aquaculture water body; s2, changing the water body, and adding a water body environment synchronous inducer composed of an ecdysone synthesis key precursor, a GABAergic nerve inhibitor and a chitin synthesis unit at one time; s3, then forcibly stopping feeding the larvae, and then feeding fortified baits; s4, after the larvae are intensively metamorphosed and developed into opossum shrimp larvae, changing a large amount of water to reset the culture environment. Through combination of environmental factor pretreatment, physiological function pulse type induction and a specific nutrition recovery strategy, accurate regulation and control of the larva metamorphosis process are achieved, metamorphosis development synchronism is remarkably improved, the cultivation period is shortened, and the survival rate is remarkably increased.

The application relates to a method for hatching artemia and a method for breeding macrobrachium rosenbergii. The method for hatching artemia comprises the following steps: soaking and disinfecting artemia eggs in a first disinfectant, cleaning the artemia eggs with water, and then hatching the artemia eggs in salt water to obtain artemia nauplii; soaking and disinfecting the artemia nauplii in a second disinfectant, cleaning the artemia nauplii with water, and then hatching the artemia nauplii in seawater containing a composite beneficial microorganism preparation to obtain artemia nauplii carrying the composite beneficial microorganism; and the composite beneficial microorganism preparation comprises lactic acid bacteria and butyric acid bacteria. The method for breeding macrobrachium rosenbergii comprises the following steps: arranging macrobrachium rosenbergii larvae in a breeding water body, feeding the artemia nauplii carrying the composite beneficial microorganism on the second day of the arrangement, and increasing the feeding amount day by day until the macrobrachium rosenbergii larvae develop to the eighth stage, and then mainly feeding egg custard and supplementarily feeding the artemia nauplii carrying the composite beneficial microorganism. The application solves the problem of low metamorphosis development rate of the larvae in the prior art method for breeding macrobrachium rosenbergii.

The invention relates to the technical field of Chinese mitten crab seed cultivation, in particular to a method for increasing the survival rate of Chinese mitten crab seed early cultivation, which comprises three key steps of pond design, equipment arrangement and juvenile crab cultivation. Pond structural design is optimized, special breeding equipment is configured, and staged refined breeding management is implemented, so that the growth requirements of the fries are accurately matched with the environment and bait; planting blocks and bait and fertilizer blocks are arranged at intervals in the pond, aquatic plant cultivation and water body pretreatment are matched, the equipment comprises a lamp belt with a bait gathering function and manual and automatic double feeders matched with different breeding stages, and bait types and feeding modes are switched according to the seedling development stage in the breeding process; the method effectively solves the survival adaptation problem of the offspring seeds in the metamorphosis shelling period and the feeding habit conversion period, is suitable for large-scale early-stage cultivation of the eriocheir sinensis offspring seeds, and has good industrial application value and popularization prospects.

The application provides a method for improving the disease resistance of Litopenaeus vannamei "Guangtai No.1", wherein (1) the water temperature is 28.3-28.7 DEG C when the Litopenaeus vannamei "Guangtai No.1" juvenile enters a breeding pond, and then is increased to 29.8-30.2 DEG C, and then is decreased to 28.8-29.2 DEG C when the M2 of Tachycaris sp. reaches, and is decreased to 27.8-28.2 DEG C when the P2 of the juvenile shrimp reaches; (2) the density is 180-200 thousand / m 3 ; (3) the feed: the metamorphosis of the nauplius into the copepodid is started, and the feed of Chateoceros muelleri and Skeletonema costatum is started, and the total density is kept as 400-600 pieces / ml; the microcapsule feed is supplemented when the copepodid is at the second stage, and the feeding frequency is 5-6 times per day; the Skeletonema costatum is fed when the copepodid is at the third stage, and the density is 90-110 pieces / ml; the microcapsule feed, shrimp slices and rotifer are fed when the Tachycaris sp. is at the first and second stages; the nauplius of Artemia is fed twice and the micro-particle feed is fed four times when the Tachycaris sp. is at the third stage; the nauplius of Artemia is fed four times and the micro-particle feed is fed twice when the juvenile shrimp is at the first stage. The application effectively improves the disease resistance of the shrimp fry, and improves the survival rate and the qualified rate.

The invention relates to the technical field of aquaculture, in particular to a culture method for improving the adhesion rate and survival rate of larvae of sea urchins, which comprises the following steps: culturing fertilized eggs of the sea urchins in a culture water body; when the larvae grow to the later stage of prismatic larvae, a chemical induction system and an adhesion substrate material are synchronously added into the culture water body; the chemical microenvironment is maintained in the attachment stage and the post-attachment stage, and stable attachment and metamorphosis development of the sea urchin larvae are promoted. According to the method, the chemical induction system and the physical adhesion substrate are synchronously added and have a synergistic effect, so that the defects of dispersed induction signals and unstable microenvironment are effectively overcome; the microcapsule can realize directional slow release of gamma-aminobutyric acid, ions, amino acid and other induction components in the surface area of the substratum, so that a long-term, stable and proper-concentration efficient induction microenvironment is established and maintained around the larva, and synchronous and synergistic improvement of the adhesion rate and the later survival rate of the sea urchin larva is realized.

The application belongs to the technical field of jellyfish prevention and control, and discloses application of alpha-Lindenic acid in inhibiting metamorphosis development process of Aurelia coerulea, and establishes an experimental model for intervention of alpha-Lindenic acid in metamorphosis development of A.coerulea. When alpha-Lindenic acid is mixed with 5-methoxy-2-methylindole to induce polyp metamorphosis development of Aurelia coerulea, the mixed solution with a final concentration of 1 μM obviously inhibits the process of metamorphosis development of Aurelia coerulea. In addition, alpha-Lindenic acid reduces the number of discoid body release, and provides a new theoretical basis for preventing jellyfish outbreak.

The application belongs to the technical field of aquaculture, and particularly relates to a method for improving the quality of gametes of Crassostrea gigas. Gonad undeveloped parent oysters of Crassostrea gigas are selected, and the parent oysters are treated with lipopolysaccharide, and then the gametes generated by the parent oysters are used to cultivate offspring after temperature promotion. The diameter of the gametes generated by the parent oysters of Crassostrea gigas treated with lipopolysaccharide is increased, the total antioxidant capacity is improved, and the growth speed and the attachment metamorphosis capacity of the offspring larvae are significantly improved. The application opens up a new way for solving the bottleneck problem of high mortality of larvae in the process of artificial breeding of Crassostrea gigas.

The invention discloses a flavobacteria bacterium capable of efficiently promoting attachment and metamorphosis of coral larvae and application of the flavobacteria bacterium. The Flavobacterium sp. SCSIO 2518 is preserved in the Guangdong Microbial Culture Collection Center on November 14, 2025, the address is Courtyard 100, Xianlie Middle Road, Guangzhou, the postcode is 510070, and the preservation number is GDMCC No.67297. The Flavobacterium sp. SCSIO 2518 is preserved in the Guangdong Microbial Culture Collection Center on November 14, 2025. The flavobacteria bacterium 2518 is separated and purified from calcified algae collected in a coral reef area of deer in trianya in Hainan province in China, can remarkably improve the adhesion rate and the metamorphosis rate of madrepore larvae and shorten time, and is free of toxic and side effects. Therefore, the method can be applied to promoting attachment and metamorphosis of the coral larvae, promoting artificial large-scale breeding of the corals and providing high-quality seedling resources for coral population recovery, so that restoration of a degenerated coral reef ecological system is promoted.