38 results about "Metamorphosis" patented technology

The invention discloses a composite inducer for promoting adhesion and metamorphosis of marine bivalve mollusk larvae and an application method of the composite inducer. The composite inducer for promoting adhesion and metamorphosis of the marine bivalve mollusk larvae comprises glutamine, glutamic acid, potassium chloride, calcium chloride and taurine, and the concentration ratio of the glutamine to the glutamic acid to the potassium chloride to the calcium chloride to the taurine is 1: 1: 2 * 10: 1.7 * 10: 4. According to the application, glutamine, potassium ions, calcium ions, glutamic acid and taurine are matched, the application effect of the glutamine, the potassium ions, the calcium ions, the glutamic acid and the taurine in the aspect of improving the attachment and metamorphosis rate of the shellfish larvae is defined, the technical blank of multi-component cooperative regulation and control of the attachment and metamorphosis of the shellfish at present is filled, a new solution is provided for bivalve shellfish fry production, and the application prospect is wide. The method is of great significance in promoting healthy development of the mariculture industry.

The application discloses a construction method and application of an ovary cell line of Quasipaa spinosa. The method comprises the following steps: selecting metamorphosis period larva ovary tissue, disinfecting and obtaining the ovary tissue, and then using collagenase I digestion solution to digest the ovary tissue at 26 DEG C for 1 hour; after the tissue block adheres to the wall, using a low-osmotic-pressure complete culture medium to carry out primary culture at 25-27 DEG C, wherein the culture medium is 60% diluted DMEM / F12 culture medium containing 10%-15% fetal bovine serum; when the cell confluence degree is higher than 70%, using trypsin-EDTA to digest the cells for 1-2 minutes to subculture, and the subculture culture medium contains 30% cell conditional culture medium; and finally, the cells are cryopreserved through programmed cooling. The ovary epithelial cell line of Quasipaa spinosa is successfully established for the first time, the survival rate of the cells after recovery is high, and the karyotype is stable. The constructed cell line can be used for amphibian reproductive biology research, compound screening and in-vitro conservation of germplasm resources.

The application relates to the field of aquaculture, and particularly discloses a method for cultivating oyster larvae, which comprises the following steps: S1, fertilized eggs are developed into D-shaped larvae in seawater at 22 DEG C for 20-24 hours; S2, the D-shaped larvae are cultivated under the condition that the temperature is 22-24 DEG C and the salinity is 29-31 ‰, feed and growth nutrient solution are fed daily, water is changed daily, after the D-shaped larvae appear eye spots, a larval collector is put in, and algal feed and growth promoting solution are fed daily, the larvae are cultivated into 0.8-1 mm juvenile shellfish, and are transferred to sea cultivation; the growth nutrient solution comprises the following components in weight parts: 1-2 parts of a small molecule peptide solution, 0.01-0.03 parts of neomycin sulfate powder, 0.5-1 part of corn starch, 2-4 parts of modified montmorillonite, and 10-15 parts of water. The method for cultivating oyster larvae has the advantages that the survival rate of the larvae is improved, the growth speed is accelerated, and the metamorphosis and attachment rate is improved.

The invention relates to the technical field of artificial cultivation of oysters, in particular to a method for artificially cultivating oysters. Comprising the following steps: (1) selecting oyster parents, and after ripening, carrying out artificial insemination and hatching to obtain oyster larvae; a ripening agent is used during ripening; (2) cultivating the oyster larvae obtained in the step (1), feeding the oyster larvae with initial feed to obtain young oysters, and feeding the young oysters with artificial feed after the young oysters are attached and metamorphosed; (3) performing intermediate culture on the oyster seedlings obtained in the step (2) to obtain juvenile mollusks; and (4) transferring the juvenile mollusks to a breeding farm to breed for 12-18 months. According to the method, high yield, high efficiency and high quality of oyster culture are achieved through an innovative ripening technology, precise nutrition allocation and scientific environment management, a reliable solution is provided for sustainable development of the oyster industry, and the method has wide application prospects.

The invention belongs to the field of shellfish culture, and particularly relates to a method for efficiently promoting chlamys farreri larva attachment and metamorphosis through levodopa. Aiming at the problem of low attachment and metamorphosis rate in the current chlamys farreri breeding process, the invention provides the method for efficiently promoting the attachment and metamorphosis of the chlamys farreri larvae by using the chemical inducer levodopa, and a technical support is provided for improving the attachment and metamorphosis rate in the chlamys farreri seedling production process.

The application discloses a kind of Phascolosoma esculenta seedling cultivation methods, it is related to the technical field of mariculture.(1) parent culture;(2) labor induction;(3) sea ball larva cultivation;(4) the preparation of benthic cultivation pool;(5) planktonic larva metamorphosis induction;(6) the cultivation management of benthic larva.The application can effectively improve the efficiency of feeding sea mud of creeping stage larva, improve the dissolved oxygen in silt substrate, reduce the low dissolved oxygen caused by accumulated water during the process of dry, and the high mortality rate.

The invention discloses an artificial maturity promoting and fry breeding method for Peltella corii, which comprises the steps of cooling temporary rearing, maturity promoting, mating, spawning, hatching, high-density larva breeding, metamorphosis and the like, male snails and female snails are separately bred during maturity promoting, and a breeding scheme is optimized. According to the technical scheme, batch gonad maturation of parent snails according to plans is achieved, and the maturity is good; high-density breeding technologies such as fertilized egg hatching and larva breeding are overcome, a stable and efficient fry production process is established, a large number of high-quality, healthy and regular-specification young snails are provided for the breeding industry, and the provenance basis of the industry is fundamentally guaranteed.

The invention belongs to the field of shellfish culture, and particularly relates to a method for efficiently promoting chlamys farreri larva attachment and metamorphosis through noradrenaline. Aiming at the problem of low attachment and metamorphosis rate in the current chlamys farreri breeding process, the invention provides the method for efficiently promoting the attachment and metamorphosis of the chlamys farreri larvae by using the chemical inducer noradrenaline, and a technical support is provided for improving the attachment and metamorphosis rate in the chlamys farreri seedling production process.

The present invention provides a method for producing artificial seeds of whelks that enables year-round production by hatching larvae from egg masses obtained through spawning and implementing appropriate management according to larval development stages, thereby reducing mortality rates and increasing production yields. By managing hatched larvae according to early, middle, and late stages, it is possible to reduce mass mortality occurring during the artificial seed production process. Furthermore, by analyzing the correlation between the installation of chillers and the replacement cycle of housing filters for water quality control and water purification efficiency, it is possible to prevent the influx of external pathogens (ciliates, scuuchica) caused by rapid environmental changes during periods of high water temperature. Additionally, by artificially inducing larval settlement, the metamorphosis period can be shortened and growth variations between individuals can be reduced. Moreover, it is possible to establish a complete whelk aquaculture system that progresses from seed production to intermediate rearing to main rearing.

The present invention discloses a variant nose cone of a cell-metamorphosis mechanism containing a coupling chain. The shell assembly includes four shells; a telescopic chain is installed on the second to fourth shells to constrain the telescopic deformation of the second to fourth shells. When the telescopic chain appears in a singular position during the telescopic deformation of the shell assembly, the telescopic chain can self-lock and unlock through its own local degree of freedom; a telescopic drive chain is arranged on the second to third shells; a bending chain is installed on the first to second shells to constrain the bending deformation of the first to second shells. When the bending chain appears in a singular position during the bending deformation of the shell assembly, the bending chain can self-lock and unlock through its own local degree of freedom; a bending drive chain is arranged on the first to second shells. The present invention can perform telescopic and bending deformation, can improve the overall load-bearing capacity of the variant nose cone, and is conducive to the variant nose cone reliably adapting to different environments.

Pest and metamorphosis stage detection instrument for agricultural use

The invention discloses a method for promoting attachment of oyster larvae by using sound waves with specific frequency. According to the method, sound waves or vibration waves with the basic frequency located at the low frequency band are applied to culture water containing the oyster larvae, so that the larvae are attached and metamorphosed on an attachment substrate, and preferably, the oyster larvae are the oyster larvae in the attachment induction period. According to the invention, under the condition of being close to natural reef sound intensity, the condition that specific low-frequency (preferably about 20 Hz) sound waves have a remarkable and repeatable promoting effect on attachment of oyster larvae is found through system screening for the first time, and medium-high-frequency sound, especially high frequency above kilohertz, has a tendency of inhibiting and even damaging the attachment of the larvae is revealed; and a new technical path is provided for regulating and controlling the behaviors of the bivalve larvae by using an acoustic means.

This invention relates to the field of artificial breeding technology, specifically to a feed additive for the fully artificially bred Northeast Forest Frog and its application. The feed additive includes dandelion and Bacillus licheniformis, wherein the mass ratio of dandelion to Bacillus licheniformis is (0.02~0.1):1. The feed additive provided by this invention can effectively control digestive diseases in forest frog tadpoles, improve intestinal flora, promote weight gain and digestion in forest frog tadpoles, significantly improve the metamorphosis effect of forest frog tadpoles, and increase the survival rate of metamorphosed forest frog larvae.

The invention discloses a construction method and application of an ovarian cell line of hapalogenys nitens. The method comprises the following steps: selecting a metamorphosis stage larva ovarian tissue, disinfecting and obtaining, and digesting for 1 hour at 26 DEG C by using collagenase I digestive juice; after the tissue block adheres to the wall, a low-osmotic-pressure complete medium is used for primary culture at the temperature of 25-27 DEG C, and the medium is a 60% diluted DMEM / F12 medium containing 10%-15% of fetal calf serum; after the cell confluence degree exceeds 70%, trypsin-EDTA (Ethylene Diamine Tetraacetic Acid) is used for digesting for 1-2 minutes for passage, and a passage culture medium contains 30% of a cell condition culture medium; and finally performing programmed cooling and cryopreservation. According to the method, the ovarian epithelial cell line of the hapalogenys nitens is successfully established for the first time, the cell recovery survival rate is high, and the karyotype is stable. The constructed cell line can be used for amphibian reproductive biology research, compound screening and germplasm resource in-vitro preservation.

The invention belongs to the technical field of shellfish genetic breeding, and particularly relates to a cultivation method of triploid offspring seeds for promoting growth of mytilus coruscus. According to the cultivation method, when the proportion of first polar bodies in fertilized eggs of mytilus coruscus accounts for 35%-40% of the total fertilized eggs, a cytochalasin B (CB) solution induces the fertilized eggs of mytilus coruscus, and then incubation and development are carried out. According to the mytilus coruscus triploid offspring seeds cultivated through the cultivation method, the triploid rate can reach 84%, the growth rate is improved by 30%-40% compared with that of diploid, larvae enter an attachment metamorphosis stage in advance, the seedling cultivation cost is greatly reduced, and a new direction is provided for industrialization development of mytilus coruscus.

The application belongs to the technical field of aquaculture, and particularly relates to a method for improving the attachment rate and disease resistance of long oyster (Crassostrea gigas) nauplii and application thereof. The method is that early nauplii of the long oyster are soaked in filtered seawater containing a certain dose of glutathione. Compared with a control group without adding glutathione, the growth and development speed of the long oyster nauplii soaked in the glutathione is accelerated, the attachment rate is significantly improved, the lysozyme activity is enhanced, and the ability to resist vibrio is significantly increased. The application has a significant effect on reducing the mortality of the long oyster nauplii in the attachment metamorphosis process and improving the attachment metamorphosis rate, and can be used as an immune enhancer for the attachment metamorphosis stage of the long oyster and applied to seed production.

The invention relates to the technical field of aquaculture, in particular to a rana nigromaculata early breeding and froglet breeding method and system. On the basis of temperature control and water body stimulation of'fullness, drying and refullness', awakening and delivery are promoted under the condition of no exogenous hormone; a stepped terrain is constructed in the same temperature control greenhouse, the water level and the water-land ratio are adjusted in a linkage mode according to a population metamorphosis rate threshold value, and continuous operation of incubation, cultivation, metamorphosis and food domestication is achieved in cooperation with a water inlet and outlet net bag with the replaceable mesh number, an anti-escape fence and moving / film covering food tables on the two sides. Compared with a conventional pond dividing mode, breeding can be advanced by more than or equal to 2 months, and froglets appear on the market to July; the survival rate of incubation and metamorphosis is improved, drowning is remarkably reduced, the success rate of domestication and the specification uniformity are improved, meanwhile, carrying stress is reduced, and the unit fry cost is reduced.

The invention discloses bacillus subtilis cell wall protein as well as an extraction method and application thereof. According to the method, bacteria are crushed by ultrasonic waves, cell wall protein is separated by a dialysis bag purification method, the original structure of the cell wall protein can be kept, the purity of a sample is improved, and the extraction yield is greater than 19%; meanwhile, the bacillus subtilis cell wall protein can improve intestinal health of bullfrog tadpoles, and the problems of low growth speed and low metamorphosis rate in the bullfrog tadpole breeding process are solved.

The invention relates to the technical field of artificial breeding, in particular to a feed additive for fully artificially bred rana chensinensis and application of the feed additive. The feed additive comprises dandelion and bacillus licheniformis, and the mass ratio of the dandelion to the bacillus licheniformis is (0.02-0.1): 1. The feed additive provided by the invention can effectively control the occurrence of digestive diseases of wood frog tadpoles, improve intestinal flora, promote weight gain and digestion of wood frog tadpoles, and significantly improve the metamorphosis effect of wood frog tadpoles and the survival rate of metamorphosed wood frog larvae.

The invention relates to the technical field of aquaculture, and discloses an industrial aquaculture workshop shell shrimp larva metamorphosis development cultivation method, which comprises the following steps: S1, when zoeae enters a metamorphosis development critical window, carrying out hypotonic period stress pretreatment on an aquaculture water body; s2, changing the water body, and adding a water body environment synchronous inducer composed of an ecdysone synthesis key precursor, a GABAergic nerve inhibitor and a chitin synthesis unit at one time; s3, then forcibly stopping feeding the larvae, and then feeding fortified baits; s4, after the larvae are intensively metamorphosed and developed into opossum shrimp larvae, changing a large amount of water to reset the culture environment. Through combination of environmental factor pretreatment, physiological function pulse type induction and a specific nutrition recovery strategy, accurate regulation and control of the larva metamorphosis process are achieved, metamorphosis development synchronism is remarkably improved, the cultivation period is shortened, and the survival rate is remarkably increased.

The application relates to a method for hatching artemia and a method for breeding macrobrachium rosenbergii. The method for hatching artemia comprises the following steps: soaking and disinfecting artemia eggs in a first disinfectant, cleaning the artemia eggs with water, and then hatching the artemia eggs in salt water to obtain artemia nauplii; soaking and disinfecting the artemia nauplii in a second disinfectant, cleaning the artemia nauplii with water, and then hatching the artemia nauplii in seawater containing a composite beneficial microorganism preparation to obtain artemia nauplii carrying the composite beneficial microorganism; and the composite beneficial microorganism preparation comprises lactic acid bacteria and butyric acid bacteria. The method for breeding macrobrachium rosenbergii comprises the following steps: arranging macrobrachium rosenbergii larvae in a breeding water body, feeding the artemia nauplii carrying the composite beneficial microorganism on the second day of the arrangement, and increasing the feeding amount day by day until the macrobrachium rosenbergii larvae develop to the eighth stage, and then mainly feeding egg custard and supplementarily feeding the artemia nauplii carrying the composite beneficial microorganism. The application solves the problem of low metamorphosis development rate of the larvae in the prior art method for breeding macrobrachium rosenbergii.

The invention relates to the technical field of Chinese mitten crab seed cultivation, in particular to a method for increasing the survival rate of Chinese mitten crab seed early cultivation, which comprises three key steps of pond design, equipment arrangement and juvenile crab cultivation. Pond structural design is optimized, special breeding equipment is configured, and staged refined breeding management is implemented, so that the growth requirements of the fries are accurately matched with the environment and bait; planting blocks and bait and fertilizer blocks are arranged at intervals in the pond, aquatic plant cultivation and water body pretreatment are matched, the equipment comprises a lamp belt with a bait gathering function and manual and automatic double feeders matched with different breeding stages, and bait types and feeding modes are switched according to the seedling development stage in the breeding process; the method effectively solves the survival adaptation problem of the offspring seeds in the metamorphosis shelling period and the feeding habit conversion period, is suitable for large-scale early-stage cultivation of the eriocheir sinensis offspring seeds, and has good industrial application value and popularization prospects.

The application provides a method for improving the disease resistance of Litopenaeus vannamei "Guangtai No.1", wherein (1) the water temperature is 28.3-28.7 DEG C when the Litopenaeus vannamei "Guangtai No.1" juvenile enters a breeding pond, and then is increased to 29.8-30.2 DEG C, and then is decreased to 28.8-29.2 DEG C when the M2 of Tachycaris sp. reaches, and is decreased to 27.8-28.2 DEG C when the P2 of the juvenile shrimp reaches; (2) the density is 180-200 thousand / m 3 ; (3) the feed: the metamorphosis of the nauplius into the copepodid is started, and the feed of Chateoceros muelleri and Skeletonema costatum is started, and the total density is kept as 400-600 pieces / ml; the microcapsule feed is supplemented when the copepodid is at the second stage, and the feeding frequency is 5-6 times per day; the Skeletonema costatum is fed when the copepodid is at the third stage, and the density is 90-110 pieces / ml; the microcapsule feed, shrimp slices and rotifer are fed when the Tachycaris sp. is at the first and second stages; the nauplius of Artemia is fed twice and the micro-particle feed is fed four times when the Tachycaris sp. is at the third stage; the nauplius of Artemia is fed four times and the micro-particle feed is fed twice when the juvenile shrimp is at the first stage. The application effectively improves the disease resistance of the shrimp fry, and improves the survival rate and the qualified rate.

The present application relates to the field of animal breeding, and particularly to a method for gender reversal of crustacean animals. The present application provides a method for gender reversal of crustacean animals, taking crustacean animals after metamorphosis, feeding with steroid hormones, and breeding. The experiment proves that sex hormones of vertebrates play a role in gender differentiation of crustacean animals, and when the concentration of MT added in feed is 2000 mg / kg, PL2 and PL25 male juvenile prawns can be induced; when the concentration of E2 added in feed is 2000 mg / kg, PL25 juvenile prawns can be induced to be female, and male juvenile prawns of PL30 can be reversed to female prawns. The experiment determines the best gender reversal period and hormone concentration in different development stages of juvenile prawns, and observes the egg-carrying "pseudo-female prawns", which lays a foundation for determining the chromosome pairing of juvenile prawns and monosex breeding.

The invention discloses pseudoalteromonas for efficiently inducing attachment and metamorphosis of sea cucumber larvae and application of the pseudoalteromonas. The Pseudoalteromonas sp. YTU123 is preserved in the China Center for Type Culture Collection (CCTCC) on September 9, 2024, the address is Wuhan University, Wuhan, China, the postcode is 430072, and the preservation number is CCTCC NO: M 20241942. The Pseudoalteromonas sp. YTU123 is named as Pseudoalteromonas sp. YTU123, and the Pseudoalteromonas sp. YTU123 is named as Pseudoalteromonas sp. YTU123. The Pseudoalteromonas sp. YTU123 is separated and purified from offshore seawater of Guizi Island in Yitai City of Shandong Province in China, and the Pseudoalteromonas sp. YTU123 can efficiently induce attachment and metamorphosis of sea cucumber larvae, so that synchronous metamorphosis of sea cucumber seedlings can be promoted, the attachment and metamorphosis rate of the sea cucumber seedlings can be remarkably increased, high-quality seedling resources are provided for sea cucumber culture, and the sea cucumber larvae can be effectively cultured. The development of the sea cucumber breeding industry in China is promoted.

The present application relates to the technical field of aquatic genetic breeding, and particularly relates to a breeding method and system for breeding a new low-salinity breeding line of Macrobrachium rosenbergii based on BLUP family selection. The present application sets up ion compensation paths and non-compensation paths in parallel salinity steps for the same family at a metamorphosis key window, obtains metamorphosis completion rate and recovery survival rate at each salinity, determines ion compensation critical salinity and non-compensation critical salinity, and constructs an ion compensation dependency coefficient. Environmental deviation is calculated in combination with water quality and pathogen and other environmental indexes, and records are weighted and gated, BLUP genetic evaluation is implemented based on weighted data, a selection index considering target salinity out-pool rate, non-compensation critical salinity and dependency coefficient is constructed, and a new line capable of being stably bred at a target low salinity and having low ion compensation dependency is obtained through iterative selection under inbreeding constraint, so that cost reduction and emission reduction and large-scale popularization are realized.

The invention relates to the technical field of aquaculture, in particular to a culture method for improving the adhesion rate and survival rate of larvae of sea urchins, which comprises the following steps: culturing fertilized eggs of the sea urchins in a culture water body; when the larvae grow to the later stage of prismatic larvae, a chemical induction system and an adhesion substrate material are synchronously added into the culture water body; the chemical microenvironment is maintained in the attachment stage and the post-attachment stage, and stable attachment and metamorphosis development of the sea urchin larvae are promoted. According to the method, the chemical induction system and the physical adhesion substrate are synchronously added and have a synergistic effect, so that the defects of dispersed induction signals and unstable microenvironment are effectively overcome; the microcapsule can realize directional slow release of gamma-aminobutyric acid, ions, amino acid and other induction components in the surface area of the substratum, so that a long-term, stable and proper-concentration efficient induction microenvironment is established and maintained around the larva, and synchronous and synergistic improvement of the adhesion rate and the later survival rate of the sea urchin larva is realized.

The application belongs to the technical field of jellyfish prevention and control, and discloses application of alpha-Lindenic acid in inhibiting metamorphosis development process of Aurelia coerulea, and establishes an experimental model for intervention of alpha-Lindenic acid in metamorphosis development of A.coerulea. When alpha-Lindenic acid is mixed with 5-methoxy-2-methylindole to induce polyp metamorphosis development of Aurelia coerulea, the mixed solution with a final concentration of 1 μM obviously inhibits the process of metamorphosis development of Aurelia coerulea. In addition, alpha-Lindenic acid reduces the number of discoid body release, and provides a new theoretical basis for preventing jellyfish outbreak.

The present application relates to aquaculture technology, in particular to a method for improving the growth and development speed and antibacterial ability of long oyster larvae by using taurine. The taurine soaking treatment of long oyster shell top larvae can promote the growth of larvae, accelerate the attachment metamorphosis process, and improve the survival rate after infection of Vibrio splendidus. The present application proves that taurine can improve the growth and development speed and immune defense ability of long oyster larvae, and has broad application prospects in shortening the oyster breeding cycle, improving the success rate and seed quality of breeding, promoting the green development of oyster culture industry, etc.

The invention discloses an efficient oyster spawning induction method based on a physiological effect. The activated oyster seminal fluid is guided to the side of the to-be-parturited open oysters with mature gonads, the oysters start to discharge sperms and eggs through seminal fluid stimulation, and parturition induction is completed. The method is simple to operate and easy to implement, and has the advantages of high induced spawning success rate, short time, large spawning amount, good effect and the like. In addition, the method is gentle and gentle, does not generate strong stimulation and damage to the parent shellfish, and indexes such as the hatching rate of fertilized eggs, the metamorphosis rate and the repeated utilization rate of the parent shellfish are averagely improved by 20% or above compared with the prior art; meanwhile, the problems of poor high-quality parents and fry breeding in the large-scale production process of the oysters are indirectly solved. In conclusion, the operation method is simple, low in cost, high in practicability, good in ecological and economic benefits and suitable for large-scale application and popularization.