169 results about "Low dissolved oxygen" patented technology

The invention discloses a device and a method for denitrification of single stage autotroph in low-cellulose nitrate (CN) high-ammonia nitrogen waste water. The device comprises a raw water tank, a water inlet pump, a reactor, a secondary sedimentation tank and a sludge reflux pump, wherein overcurrent holes are arranged in the water flow direction of the reactor in an up-and-down alternative form and connected with various grid chambers, an anoxic zone grid chamber is arranged at the front end of the reactor, and an aerobic zone grid chamber is arranged at the back end of the reactor; the anoxic zone grid chamber is provided with a stirrer and an agitator blade; the aerobic zone grid chamber is provided with the stirrer, the agitator blade, an aeration riser pipe and an intermediate perforated clapboard; and the aeration riser pipe is internally provided with an aeration head, and sponge filling material is filled below the intermediate perforated clapboard, wherein filling ratio is 30-50%. In the method, shortcut nitrification is achieved above the aerobic zone through low dissolved oxygen (DO is 0.5 <-1>mg/L) and free ammonia (FA) inhibition so that ammonia nitrogen is converted to nitrite nitrogen; and anoxicammoxidation biomembrane acts on the lower part of the aerobic zone, the nitrite nitrogen and ammonia nitrogen are converted to nitrogen, thereby achieving denitrification of autotroph. The method has the advantages of low oxygen consumption, less sludge output and no extra carbon source.

Methods and systems are provided for reducing ammonia partially by a denitrification process. A stream containing ammonia is contacted with oxygen to form a first product stream in low dissolved oxygen conditions. The ratio of oxygen to nitrogen is about 2.28 g O₂/g N—NH₃ (2.28 grams of oxygen per gram of nitrogen in ammonia) or less. The first product stream is then exposed to organic matter in an amount of 0.57 g COD/g N—NH₃ (0.57 grams of chemical oxygen demand per gram of nitrogen in ammonia). This microbial reaction ultimately produces nitrogen gas, water, and carbon dioxide.

The invention provides reinforced simultaneous nitrogen and phosphorus removal sewage treatment equipment and a method for municipal sewage with a low carbon source at low oxygen concentration. The equipment is composed of an SBR main reaction tank and two dephosphorizing tanks, wherein, the water inlet pipe of the SBR main reaction tank is led in from the upper part of the reaction tank, the water outlet pipe thereof is led out from the lower part of the reaction tank, and an external circulation sludge pipe is led out from the bottom of the SBR main reaction tank; the SBR main reaction tank is equipped with an aerating apparatus; and the SBR main reaction tank adopts an operation mode of water inflow in batches, and anaerobic-aerobic/anoxic alternation and sedimentation and drainage. The two dephosphorizing tanks have identical structure size and are respectively taken as an anaerobic phosphorus release tank and a chemical dephosphorizing tank in the same cycle for sewage treatment, and functions of the two tanks are exchanged in the next cycle. The invention can well realize reinforced simultaneous nitrogen and phosphorus removal treatment for the municipal sewage with a low carbon source under a low dissolved oxygen environment.

The invention belongs to the treatment field of waste water, and provides a method for converting active sludge into autotrophic denitrified sludge based on a mixed type continuous flow reactor. The method which is used for converting the common active sludge into the autotrophic denitrified sludge by the continuous flow reactor specifically comprises two stages. The first stage comprises the steps of: converting anaerobic or anoxic sludge into a nitrosation status by the condition which controls the organic matter concentration (COD (chemical oxygen demand) is less than 20mg/L), the water dissolved oxygen concentration (1-0.5mg/L), and the inflowing ammonia nitrogen concentration (50-450mg/L), and restraining the growth of the other microorganisms step by step by the running condition and the washing characteristic of the continuous flow reactor. When the cumulative percentage of the nitrite is more than 60%, the second stage is started. The second stage comprises the step of: promoting the nitrosation status to be converted into the autotrophic denitrification status by the condition which improves the ammonia nitrogen concentration (450-550mg/L), reduces the dissolved oxygen (0.5mg/L), and keeps a certain PH value (7.5-8.0) to form a system which is coupled nitrosified microorganism to anammox microorganism. The practice proves that the system can stably run for a long term. After entering into the practical running stage of the waste water treatment, the method leads the ammonia nitrogen removing rate and the total nitrogen removing rate to be respectively more than 80% and 70% by the reaction which is remained only by the natural reoxygenation or a small quantity of aeration without aeration.

The invention relates to a prawn low dissolved oxygen resistance family selection method, and belongs to the technical field of prawn stress-resistance trait selection. The method comprises the steps of base population establishing; F1 generation family establishing; same pool culturing; low dissolved oxygen resistance testing; F2 generation family establishing; continuous generation selecting, wherein different sources of parent prawns are selected to establish a full/half-slib family through directional mating, common environmental breeding is performed, 94 h low dissolved oxygen stress is performed, parent selecting and remaining are performed according to the low dissolved oxygen resistance test result and the growth measure result, after four generations of continuous selection, the family of distant genetic relationship is selected from the families with excellent growth performance and strong low dissolved oxygen resistance performance, the male and female prawns which are big in individual, strong in vigor and normal in gonad development are selected to serve as parent to breed as good varieties. According to the method, the problem occurring in prawn high-density culturing that the prawns are dead caused by the fact that the prawn growth is influenced by low dissolved oxygen is solved, and the development of the prawn high-density culture industry is greatly promoted.

The present invention relates to an improved MUCT process and equipment, belonging to the field of sausage treatment technology. It is characterized by that the aerobic reactor is divided into two stages, between two aerobic reactors a deoxygenation reactor is set,and every aerobic reactor is equipped with an independent gas flow controller. In first aerobic tank it can control low dissolved oxygen to implement short range nitrification and make ammoniac nitrogen be oxidated into nitrite, in second aerobic tank it applies high dissolved oxygen to ensure that the residual nitrite can be oxidated into nitrate. Said equipment also includes a second an aerobic reactor. Besides, said invention also provides the concrete function and action of the deoxygenation reactor and said second anaerobic reactor.

The invention discloses an energy-saving consumption-reducing short-range nitration dephosphorizing coupling technique with synchronous denitrification and a device thereof, belonging to technical field of domestic and industrial sewage treatment. A metal carrier bed is installed in an aerobic section with integrated reaction, and is made of iron wood shaving washed through acid cleaning; and the outer part of the iron wood shaving is covered by wire netting. Under conditions of normal temperature and low dissolved oxygen, the aims of short-range nitration and synchronous denitrification can be realized; an anaerobic section is used for finishing phosphorous releasing; the aerobic section is used for finishing short-range nitration, an anoxic section is used for finishing denitrification for removing phosphorous, a fast aerobic section is used for removing remaining ammonia and nitrogen and phosphorous. The invention uses iron wood shaving with low cost to realize stable accumulation of nitrite; the aerating amount is small, the carbon source is saved, and denitrification for phosphorous removing is carried out by taking the nitrite produced via short-range nitration as electron donor, thus the nitrogen and phosphorous can be removed synchronously; and the problem that the nitrite in water could damage the environment and has toxicity is solved. The invention can realize the aims of saving energy and reducing consumption to the largest extent, and is suitable for purifying and treating domestic sewage continuously for a long time.

The invention relates to a hydraulic power oxygenation constructed wetland, belonging to the technical field of constructed wetland, waterbody ecosystem restoration and sewage treatment. A plurality of monolater modules of the hydraulic power oxygenation constructed wetland are in a stepped shape to construct the hydraulic power oxygenation constructed wetland; and each level constructed wetland is composed of a water intake device, a wetland main body and a water discharging device. The wetlands in the adjacent two layers are linked with a water intake and discharging connection device so as to ensure that the processed waterbody flows through a first level, a second level...nlevel hydraulic power oxygenation constructed wetland successively and is exhausted after carrying out the n numbered level processing. The invention solves the problems of low dissolved oxygen level of constructed wetland matrix, sludge plugging of the matrix, unstable operation and the like, improves microorganism activity of the bed body, and forms a new sewage processing technology with high efficiency, stability, energy saving and perennial operation. The invention is suitable for the aspects of distributed domestic sewage processing, non-point source control, tail water processing of sewage plant, the waterbody ecological restoration engineering and zoology slope protection.

The invention relates to an SNP marker related with low dissolved oxygen survivability of litopenaeus vannamei, screening and applications thereof, and belongs to the technical field of molecular marker assisted selection of aquatic animals. An SNP molecular marker is cloned from SOD gene. The SNP marker is that the R in the 118-th base from the 5' terminal of the nucleotide sequence represented by SEQ ID No.1 is C or T. The SNP marker is prominently related with the character of tolerating low dissolved oxygen of litopenaeus vannamei, and thus can be applied to the molecular marker assisted selection so as to screen litopenaeus vannamei with strong performance of tolerating low dissolved oxygen. The provided SNP marker can be used to carry out early stage screening according to the actual needs, thus the efficiency and accuracy of breeding are effectively improved, the genetic level of litopenaeus vannamei is improved, species with strong stress resistance can be accurately and efficiently bred, the SNP marker is practical and the application range is wide.

The invention discloses a method for rapid mutagenesis of autotrophic nitrosation sludge from complete nitrifying sludge employing a sequencing batch reactor (SBR) mode, and belongs to the fields of water environment restoration and regeneration and the field of autotrophic nitrogen removal of a sewage treatment plant. The method comprises the following steps: firstly, inoculating nitrifying sludge of a sewage plant; controlling high ammonia-nitrogen concentration, high free ammonia and high aeration condition to prevent sludge from bulking, so as to rapidly adapt to an SBR reactor; then gradually eliminating heterotrophic microorganisms under the water condition with high ammonia-nitrogen and absence of an organic carbon source; finally, inhibiting growth of autotrophic bacteria such as nitrite oxidizing bacteria (NOB) under the condition of low dissolved oxygen; purifying nitrosation sludge, so as to successfully achieve transformation from heterotrophic nitrification sludge to autotrophic nitrosation sludge. By adopting the method, the heterotrophic bacteria are eliminated, and the autotrophic nitrosification technology is developed. Compared with the traditional nitrogen removal process, the method is small in oxygen demand, low in energy consumption and free of a lot of organic carbon sources.

A method for acclimating partial nitrification sludge quickly and effectively belongs to the technical field of wastewater biological removal of nitrogen. The method includes: inoculating common flocculent sludge into a sequencing batch reactor, and regulating parameters including ammonia nitrogen loading, the pH (potential of hydrogen) value, dissolved oxygen and the like of inlet water at a room temperature to enable a reaction system to operate in a high-free-ammonia state and a low-dissolved-oxygen state. Compared with a common regulating manner by means of regulating the dissolved oxygen, the temperature and a sludge age, the method for acclimating the partial nitrification sludge quickly and effectively has the advantages that partial nitrification sludge acclimating time is shortened from 10-15 days from 60-120 days, and the method is simple to operate and stable in operation effect.

The invention provides a device and a method for starting micro expansion of sludge in the anoxic/aerobic biological denitrification process, and belongs to the technical field of sewage treatment. The method is characterized in that in an anoxic/aerobic biological denitrification system, the micro expansion of the sludge is started, the micro expansion of the sludge is controlled within a certain limit, treatment effects are optimized and energy consumption is saved. The proposal of starting the micro expansion of the sludge in the anoxic/aerobic biological denitrification system comprises: 1, triggering the micro expansion of the sludge only by low dissolved oxygen, and including two starting modes for gradually lowering the dissolved oxygen concentration and suddenly lowering the dissolved oxygen concentration; and 2, starting the micro expansion of the sludge in the mode of the low dissolved oxygen combined with the low load. The device and the method are mainly used to optimize operational effects of the anoxic/aerobic biological denitrification sewage treatment system, namely under the conditions of lowering the dissolved oxygen concentration, saving aeration rate and saving energy, the pollutant-removing rate is ensured to meet the standards, and simultaneously the concentration of effluent suspended matters is greatly reduced.

The invention discloses a screening method of denitrifying bacteria, which comprises the processes that: (1) the denitrifying bacterium group enrichment culture is carried out on aerobic activated sludge by ammonia-containing sewage; (2) the heterotrophic bacterium group culture is carried out under the condition of low dissolved oxygen; (3) heterotrophic nitrobacteria are separated and purified from the enriched heterotrophic bacterium group; (4) the purified heterotrophic nitrobacteria are subjected to growing capacity domestication; and (5) the denitrification capacity domestication is carried out on the purified thalli by the ammonia-containing sewage. The denitrifying bacteria screened by the method are applied to the sewage denitrifying treatment, the defects of the traditional biological denitrification technology can be overcome, the denitrifying bacteria can also be used as bioremediation agents to be replenished into an impacted sewage treatment system under different environment conditions, the processing capability of the system is fast restored, the nitration ammonia nitrogen removal capability is realized, simultaneously, the denitrification total nitrogen removal capability is also realized, and conditions are prepared for simultaneous nitrification and denitrification nitrogen removal.

The invention discloses a highly-efficient stable bio-doubling process sewage treatment device, which is improvement of a bio-doubling process sewage treatment device. The highly-efficient stable bio-doubling process sewage treatment device is characterized in that: an aerobic aeration area is an annular channel, a water propulsion device is arranged in the annular channel, a water-feeding mix reaction area, a water lifting area and a settlement area are internally arranged at the inner side of the annular channel, and the annular channel is respectively communicated with the settlement area and the water-feeding mix reaction area. According to the pool-type structure, the contradiction between low dissolved oxygen amount and high sludge content required by the bio-doubling process is greatly solved, the requirements on a bio-doubling process for low dissolved oxygen amount and high sludge content can be ensured, thus the practical effect and advantage representation of the bio-doubling process are ensured; two or more loops are formed through treatment, thus the involved devices are conveniently overhauled without running stop, and the reflux ratio is adjustable and high; in addition, a controllable gate or valve is additionally arranged between the lifting area and the annular channel, thus practical running adjustability is improved; and after construction or construction finish of the controllable gate or valve, an aeration device is flexibly added, or started/stopped according to water quality, thus the application expandability of the highly-efficient stable bio-doubling process sewage treatment device is good, and adaptability of the highly-efficient stable bio-doubling process sewage treatment device is strong.

A normal temperature low-ammonia-nitrogen nitrosation starting method belongs to the field of urban sewage treatment and reclamation. Researches show that nitrosation starting can be achieved in short time by controlling condition of low dissolved oxygen (DO=0.30mg/L) and inoculating sludge with certain nitrosation effect. If whole course nitration sludge is inoculated, no accumulation of nitrite occurs within 30 days in a water distribution test, no accumulation of nitrite occurs in 30 days in a reactor which utilizes the A/O dephosphorization process processing water as a research object, and the nitrosation starting fails accordingly. Nitrite accumulation can occur by adopting the mode of high-low gradient oxygen limit culture, domesticating the sludge for 10 days under the condition that DO=0.70-0.80mg/L, then controlling condition of low dissolved oxygen (0.30-0.40mg/L) to enable the reactor to discharge water if the whole course nitration sludge is inoculated. Over 90% of nitrosation rate can be achieved within 38 days by continuously domesticating the sludge under the condition of the dissolved oxygen, so that nitrosation can be successfully started.

The invention discloses a dissolved oxygen automatically controlled MBR full-course nitrifying bacteria enriching device and a method thereof. The device comprises a reactor, a water inlet barrel, a water bath pot and a control cabinet, wherein a reactor main body is a cylindrical tank body; a water inlet is formed below the tank body and dewaters through an internal membrane assembly; the tank body is filled with packing; a DO probe and a sampling port are formed above the tank body; a temperature-control jacket is arranged on the outer side of the tank body; an aerating disc is arranged in the bottom of the tank body to regulate the concentration of the dissolved oxygen in the reactor and meanwhile plays a stirring role and prevents the membrane assembly from being blocked. The device disclosed by the invention provides an environment with low dissolved oxygen and low nutritional matters for full-course nitrifying bacteria; the specific surface area of the packing filling the reactor is large, so that microorganisms are attached to grow; the membrane assembly is adopted for effluent, so that the biomass loss can be effectively prevented; the dissolved oxygen in the reactor is controlled at a relatively low level through a dissolved oxygen probe, an air pump and a nitrogen steel cylinder, so that full-course nitrifying bacteria are effectively enriched to obtain a high purity enriched culture.

The invention discloses a method for selectively producing 3-hydroxyl-2-butanone and 2, 3-butanediol by using an environmental safety bacterial strain Bacillus amyloliquefaciens with the CCTCC NO. M2012349 and changing the content of corn steep liquor in a medium, the stirring speed and the ventilation capacity, and belongs to the field of a fermentation technology in biological engineering. The method is characterized in that when the 3-hydroxyl-2-butanone is mainly synthesized, the concentration of the corn steep liquor in the fermentation medium can be reduced to be more than or equal to 5g/L, and the relatively high dissolved oxygen level (the air mass flow is 240m<3>/h.m<3> medium, and the stirring rotating speed is 450r/min) is maintained in a fermentation process; and when the 2, 3-butanediol is synthesized, the concentration of the corn steep liquor in the fermentation medium can be increased to be more than or equal to 40g/l, the relatively low dissolved oxygen level (the air mass flow is 120m<3>/h.m<3> medium, and the stirring rotating speed is 350r/min) is maintained in a fermentation process.

A technology for removing total nitrogen in garbage leachate by anammox and a special device thereof are disclosed. The technology comprises the following steps: low dissolved oxygen concentration is strictly controlled in the nitration stage to oxidize ammonia nitrogen into nitrite nitrogen; in the denitrification stage, nitrite nitrogen is converted into nitrogen to escape so as to reduce total nitrogen in garbage leachate; then, an effluent undergoes mud-water separation, and wastewater is fully mixed with a few amount of garbage leachate stock solution in order to control the ratio of ammonia nitrogen to nitrite nitrogen in the wastewater and contents; simultaneously, nitrogen or carbon dioxide is supplemented in order to reduce dissolved oxygen in water and then provide favorable flow condition and strict anaerobic condition for follow-up anammox; and finally, a hydrophilic biological stuffing with large specific surface area is arranged in the anammox reactor to provide a stable growth environment for anammox bacteria so as to smoothly carry out anammox denitrification reaction. Therefore, total nitrogen of late landfill leachate reaches the emission standard, and energy is saved and consumption is reduced.

The invention relates to a method for realizing semi-nitrosation under a condition of room-temperature municipal sewage, and belongs to the field of municipal sewage treatment and reuse. The invention aims at solving the problems of high-energy and high-material consumption during the prior biological denitrification for municipal sewage and providing proper water input for applying anaerobic ammoxidation process to the denitrogenation treatment for municipal sewage. The method is successfully applied for the first time to the biological treatment of the semi-nitrosation of municipal sewage through control of low dissolved oxygen and auxiliary addition of part of ammonia salts under the condition of room-temperature municipal sewage, thereby ensuring the application of the high-efficiency low-consumption anaerobic ammoxidation process to the biological denitrification of municipal sewage. The method has the advantages of short reactor starting cycle, simple operation, stable system running, good sewage treatment effects, low cost, and the like.

The invention relates to an enhanced nitrogen and phosphorus removal method for compound microorganisms to efficiently treat high-concentration organic wastewater. The wastewater first enters the energy release area, and the water from the overflow weir in the energy release area is divided into two paths, one path enters the strict anaerobic area, and the other path enters the biological Selection area; in the biological selection area, the sludge returned from the strict anaerobic area and the influent are fully mixed, and then enter the complex microbial reaction area; in the complex microbial reaction area, short-range nitrification and denitrification, anaerobic ammonium oxidation, and the whole process Autotrophic denitrification, simultaneous nitrification and denitrification and other processes, so as to achieve the purpose of denitrification; the waste water enters the mud-water separation area after passing through the compound microbial reaction area, and the clear water separated in the mud-water separation area enters the phosphorus removal area for phosphorus removal, and finally makes the waste water reach Discharge standards or pre-treatment standards for reused water. This method is a new breakthrough to the original traditional sewage treatment process. In the environment of low dissolved oxygen, high sludge concentration and high removal rate make the energy consumption of wastewater treatment greatly reduced.

A common final clarifier is provided downstream of a two-stage activated sludge (AS) system that includes a flow equalization basin (FEB) reactor and a nitritation reactor as the first AS stage and an anammox reactor as the second AS stage. A first return activated sludge (RAS) flow is conducted from the final clarifier to the first AS stage with a low flow rate and a second segregated RAS flow is conducted to the second AS stage with a high flow rate. The FEB reactor is operated as an anoxic reactor to achieve carbonaceous BOD removal; conversion of organic nitrogen to ammonia nitrogen; and, denitrification of recycled nitrite or nitrate nitrogen. The nitritation reactor is operated as an aerobic reactor to achieve oxidation of ammonia nitrogen to nitrite nitrogen by biological nitritation. The anammox reactor is operated at zero or very low dissolved oxygen concentration to achieve nitrogen removal by deammonification.

The invention relates to a soybean wastewater biochemical treatment process and a soybean wastewater biochemical treatment device. The process comprises the following steps and the device comprises the following composition that wastewater sequentially flows through a regulating tank, a hydrolysis acidification pool, an anaerobic tank, a biological synchronous degradation tank and a sludge-water separation device; by the hydrolysis acidification pool, particles and macromolecules are hydrolyzed into soluble substances and COD (Chemical Oxygen Demand) degradation is carried out; when methane is generated in the anaerobic tank, COD degradation is carried out in the anaerobic tank; the biological synchronous degradation tank adopts low dissolved oxygen aeration to carry out synchronous degradation of COD and N; mixed solution at the tail end of the biological synchronous degradation tank flows back to the water inlet end and part of the mixed solution is lifted to the sludge-water separation device; clear water subjected to sludge-water separation reaches the standard and is discharged, sludge flows back to the water inlet end of the hydrolysis acidification pool; and residual sludge is discharged into a sludge treatment system. The soybean wastewater biochemical treatment process and the soybean wastewater biochemical treatment device has the benefits that the whole biochemical treatment process and the biological synchronous degradation of COD and N are adopted; treated effluent reaches the A-level emission standard of 'Integrated Wastewater Discharge Standard'(GB8978-1996); and the soybean wastewater biochemical treatment process and the soybean wastewater biochemical treatment device solve the problems that in the soybean wastewater treatment, N exceeds the standard, the sludge quantity is large and cost is high.

The invention relates to a complex microbial agent for quickly treating black and odorous water in towns. The compound microbial agent is prepared from, by weight, 4-18 parts of a bacillus subtilis complex flora, 2-15 parts of a pseudomonas complex flora, 2-10 parts of a photosynthetic bacteria complex flora, 2-10 parts of a Gordonia complex flora, 2-10 parts of nutrients, 1-5 parts of hydrolase and 10-35 parts of a solid carrier. By means of the complex microbial agent, ammonia nitrogen, COD and other indexes of the black and odorous water can be quickly reduced by degradation, the high degradation efficiency can still be kept under the condition of low dissolved oxygen, and the problem of low degradation efficiency of existing complex microbial agents under the condition of low dissolvedoxygen is effectively solved.

The invention provides denitrifying strain Klebsiella sp. KSND-3 with simultaneous aerobic nitrification and denitrification performances and preparation and application thereof. The present inventionalso provides a Klebsiella strain and application thereof in river sewage and rural ammonia-containing domestic sewage. Because of fast growth rate, high cell yield and low dissolved oxygen concentration demand of the Klebsiella strain, the Klebsiella strain can be used for simultaneous nitrification and denitrification, and the denitrification process is more concise, economical and effective.

The invention relates to a sequencing batch reaction device and method for carrying out dephosphorization and partial nitrification on low-carbon urban sewage in a single-sludge system and belongs to the fields of urban sewage treatment and resource regeneration. The device comprises a sequencing batch reactor, a stirring mill and an aerator. The device comprises the following steps: controlling the sludge age, selectively discharging sewage, maintaining the low dissolved oxygen, carrying out time-controlled semi-nitrosation, preserving phosphorus-accumulating bacteria, inhibiting nitrite-oxidizing bacteria, enriching ammonia oxidizing bacteria, rapidly starting a microorganism system led by the phosphorus-accumulating bacteria and the ammonia oxidizing bacteria, and optimizing a microenvironment in which the phosphorus-accumulating bacteria and the ammonia oxidizing bacteria coexist, so as to enable the contents of water and phosphorus to reach a first-stage standard A, wherein the specific value of nitrite nitrogen to ammonia nitrogen is about 1:1; after carrying out precipitation and water discharging, creatively carrying out anaerobic stirring on the precipitated sewage, and restoring the residual nitrite nitrogen into nitrogen by virtue of endogenous denitrification. According to the device and the method, the problem that the phosphorus-accumulating bacteria and the ammonia oxidizing bacteria cannot harmoniously coexist in a single sewage system is solved, and a novel thought is provided for dephosphorization and autotrophic nitrogen removal processes.

The invention belongs to the field of environmental application microorganisms, in particular to a high-temperature micro-aerobic composting nitrogen-preserving bacterium for reducing ammonia volatilization of animal manure composting and an application thereof. The high-temperature micro-aerobic composting nitrogen-preserving bacterium is Bacillus, is named Nitrobacillus harbinensis BM62, and hasbeen deposited in the General Microbial Center of the Chinese Microbial Culture Collection Management Committee (CGMCC) and classified as Nitrobacillus harbinensis with the collection number of CGMCCNo. 16237. The microbial species are classified as Nitrobacillus harbinensis, Nitrobacillus harbinensis and Nitrobacillus harbinensis. The strain of the invention can carry out ammonia oxidation reaction under the low dissolved oxygen condition in the high temperature period of compost, and can be used for preparing the compound bacterial agent to reduce ammonia volatilization in the high temperature period of compost of animal manure, reduce unpleasant smell, improve compost fertilizer efficiency, improve compost product quality, and can be used for producing bio-organic fertilizer, therebyreducing agricultural production cost. The strain of the invention is characterized in that the ammonia oxidation reaction is carried out under the low dissolved oxygen condition in the high temperature period of compost.