High-temperature micro-aerobic composting nitrogen-preserving bacterium for reducing ammonia volatilization of animal manure composting and application thereof

A technology of livestock and poultry manure and ammonia volatilization, applied in the application, preparation of organic fertilizer, bacteria and other directions, can solve the problems of inability to adapt to low dissolved oxygen environment, limitation of physiological metabolic activity, limitation of ammonia oxidation reaction, etc., to reduce compost nitrogen Loss, reduce NH3 volatilization, reduce the effect of ammonia volatilization

Active Publication Date: 2019-01-11
NORTHEAST AGRICULTURAL UNIVERSITY
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Problems solved by technology

[0004] However, the vast majority of AOBs are mesophilic aerobic bacteria, which can only function during the compost cooling period
Moreover, typical ammonium oxidation is a necessary aerobic process, and after the start of composting, the internal environment is a high temperature and low dissolved oxygen environment most of the time, and the low dissolved oxygen environment under high temperature conditions will also severely limit the progress of the ammonium oxidation reaction.
The high-temperature ammonia oxidizing bacteria that have been reported so far are all heterotrophic aerobic bacteria. The characteristics of heterotrophy make them have survival advantages, and they can still grow well in the environment rich in organic matter such as compost; however, the characteristics of aerobic make them unable to adapt In the low dissolved oxygen environment during the high temperature period of composting, the physiological and metabolic activities are severely restricted

Method used

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  • High-temperature micro-aerobic composting nitrogen-preserving bacterium for reducing ammonia volatilization of animal manure composting and application thereof
  • High-temperature micro-aerobic composting nitrogen-preserving bacterium for reducing ammonia volatilization of animal manure composting and application thereof
  • High-temperature micro-aerobic composting nitrogen-preserving bacterium for reducing ammonia volatilization of animal manure composting and application thereof

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specific Embodiment approach 1

[0022] Embodiment 1: A high-temperature micro-aerobic composting nitrogen-holding bacterium that reduces the volatilization of ammonia in livestock and poultry manure composting. It belongs to a new genus of the Bacillus family and is named Nitrobacillus harbinensis BM62. Center (CGMCC) preservation, the preservation address is the Institute of Microbiology, Chinese Academy of Sciences, No. 1 Beichen West Road, Chaoyang District, Beijing, the classification name is Nitrobacillus harbinensis, the preservation number is CGMCCNo. It is a Gram-positive bacterium, with a single cell in the shape of a rod, with a length of about 2-2.5 μm and a width of about 0.3-0.6 μm, with flagella. The colonies are moist in texture, light yellowish brown in color, opaque, with rounded edges, smooth and shiny surface, and slightly raised.

[0023] In this embodiment, the growth temperature of the strain Nitrobacillus harbinensis BM62 is 35-65° C., the optimum growth temperature is 50° C., the grow...

specific Embodiment approach 2

[0024] Specific embodiment two: the screening method of Nitrobacillus harbinensis BM62 is realized according to the following steps:

[0025] 1. Weigh 5g samples from compost samples at 6 different temperatures (50°C, 45°C, 38°C, 30°C, 65°C, 58°C), and place them in a conical container filled with 45mL of sterile water and 100mL. Shake the bottle for 30 minutes to make a bacterial suspension. Take 10 mL of the bacterial suspension and place them in Erlenmeyer flasks containing 150 mL of enrichment medium for 4 days with constant vibration and shaking at a temperature of 50°C and a rotation speed of 150 rpm. The compost sample is: in September 2014, the cow dung compost collected from Harbin City, Heilongjiang Province, the People's Republic of China.

[0026] 2. Draw 0.5mL culture bacteria solution from the Erlenmeyer flasks after 4 days of cultivation and inoculate them on plates (streak separation medium) respectively by coating method, and place them in a microaerobic incu...

Embodiment 3

[0052] Example 3 Strain Nitrobacillus harbinensis BM62 is used for small-scale compost fermentation experiments

[0053] Take the high-temperature micro-aerobic ammonia oxidizing strain Nitrobacillus harbinensis BM62 screened out in step 3 of Example 2 and insert it into the ammonia oxidizing bacteria seed medium, shake it at 50°C and 100rpm for 2 days, and the concentration is D 600 =0.1~0.15, prepared as a liquid microbial agent of Nitrobacillus harbinensis BM62 for future use.

[0054] Fresh cow dung and powdered rice stalks (made by cutting dry rice stalks into 1-2cm long pieces) are mixed evenly in a mass ratio of 3:1 (moisture content is about 60wt%) and loaded into a size of 153cm ×297cm polyethylene bag with stopper, inoculate the liquid bacterial agent of strain Nitrobacillus harbinensis BM62 according to the inoculum amount of 10% (v / w, mL / Kg), mix evenly, leave it to ferment at room temperature (20°C), and do 3 groups in parallel Experiment, the experimental result...

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Abstract

The invention belongs to the field of environmental application microorganisms, in particular to a high-temperature micro-aerobic composting nitrogen-preserving bacterium for reducing ammonia volatilization of animal manure composting and an application thereof. The high-temperature micro-aerobic composting nitrogen-preserving bacterium is Bacillus, is named Nitrobacillus harbinensis BM62, and hasbeen deposited in the General Microbial Center of the Chinese Microbial Culture Collection Management Committee (CGMCC) and classified as Nitrobacillus harbinensis with the collection number of CGMCCNo. 16237. The microbial species are classified as Nitrobacillus harbinensis, Nitrobacillus harbinensis and Nitrobacillus harbinensis. The strain of the invention can carry out ammonia oxidation reaction under the low dissolved oxygen condition in the high temperature period of compost, and can be used for preparing the compound bacterial agent to reduce ammonia volatilization in the high temperature period of compost of animal manure, reduce unpleasant smell, improve compost fertilizer efficiency, improve compost product quality, and can be used for producing bio-organic fertilizer, therebyreducing agricultural production cost. The strain of the invention is characterized in that the ammonia oxidation reaction is carried out under the low dissolved oxygen condition in the high temperature period of compost.

Description

technical field [0001] The invention belongs to the field of environment-applied microorganisms, and in particular relates to a nitrogen-holding bacterium that reduces ammonia volatilization during the high temperature period of composting. Background technique [0002] High-temperature composting is an effective measure to realize the reduction, harmlessness and resource utilization of livestock and poultry manure. However, NH 3 Severe loss of nitrogen mainly due to volatilization is the bottleneck problem faced by the high-temperature composting technology of livestock and poultry manure. The research results show that the nitrogen loss caused by ammonia volatilization during the composting process can reach 44-99% of the total, which not only reduces the nitrogen content in the compost, but also pollutes the atmosphere, endangers the health of humans and animals, and brings acid rain hazards and water richness. Nutritization. Therefore, how to effectively reduce nitrog...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C05F11/08C05F17/00C12R1/01
CPCC05F3/00C05F11/08C05F17/00C12R2001/01C12N1/205Y02W30/40
Inventor 徐杰许修宏刘华晶李洪涛
Owner NORTHEAST AGRICULTURAL UNIVERSITY
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