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Klebsiella strain and application thereof in river sewage and rural ammonia-containing domestic sewage

A technology of Klebsiella and strains, applied in the application field of denitrification, which can solve the problems of many reaction steps, long process flow, and low cell concentration in the denitrification process, and achieve low dissolved oxygen concentration and simple denitrification process , the effect of high cell yield

Active Publication Date: 2018-07-31
ZHEJIANG SHUANGLIANG SUNDA ENVIRONMENTAL PROTECTION CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] ① Autotrophic bacteria grow slowly, with low cell concentration and poor environmental tolerance, so the reaction time is long and the denitrification efficiency is low;
[0006] ② There are many reaction steps in the whole denitrification process, the process flow is long and complicated, the site requirements are high, the investment is large, and the operation is difficult;
[0007] ③The nitrification reaction requires a large amount of oxygen, the reaction system is greatly affected by the environment, and the impact resistance is weak

Method used

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  • Klebsiella strain and application thereof in river sewage and rural ammonia-containing domestic sewage
  • Klebsiella strain and application thereof in river sewage and rural ammonia-containing domestic sewage
  • Klebsiella strain and application thereof in river sewage and rural ammonia-containing domestic sewage

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Experimental program
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Effect test

Embodiment 1

[0035] Isolation, Screening and Characterization of Heterotrophic Nitrification-Aerobic Denitrification Strains

[0036] 1. Enrichment culture: Take 10g of Yuhang Xingqiao river sludge sample in a conical flask filled with 100ml of sterile normal saline, oscillate until the sample is completely dispersed and fully mix with sterile normal saline to prepare a bacterial suspension. Take 10ml of the bacterial suspension and transfer it to 100ml of acclimatization medium, and shake and culture at 30°C and 200r / min for 2 days. This is the first acclimatization cycle. Take 10ml of the previous culture and transfer it to the second-stage acclimatization medium, and culture it with shaking at 30°C and 200r / min for 2 days.

[0037] 2. Initial screening of bacterial strains: Take 10ml of the above-mentioned bacterial solution that has been domesticated and cultivated for 24 hours, add it to a conical flask containing 90ml of sterile normal saline, and dilute the sample to 10ml according to...

Embodiment 2

[0045] Example two denitrification bacterial strain molecular biology identification

[0046] Genomic DNA of SD3 was extracted using the Microbial Genome Extraction Kit of Hangzhou Baosai Biological Co., Ltd., and the 16S rDNA fragment was amplified using this as a template. The identified primers were 27f:AGAGTTTGATCCTGGCTCAG and 1492R:GGTTACCTTGTTACGACTT. PCR reaction system (50uL): Template DNA 1uL, 2xPCR superpfu mix 25uL, 27f and 1492R primers 1uL each, add sterile deionized water until the reaction system is 50uL. PCR program: 94°C for 5min, [94°C for 30s, 55°C for 30s, 72°C for 1min and 30s] x30 cycle, 72°C for 5min, 4°C for 5min. The recovered product after PCR purification was sent to a gene sequencing company for sequencing. The obtained 16S rDNA sequence of the SD3 strain is shown in SEQ ID NO.1. According to the homologous sequence comparison analysis of NCBI database, the SD3 strain has the closest genetic relationship with Klebsiella sp, with 100% homology, an...

Embodiment 3

[0048] Klebsiella sp. KSND-3 batch culture to investigate the ammonia oxidation rate of ammonia oxidizing bacteria at different initial concentrations. The experiment was carried out in a 250ml Erlenmeyer flask with initial pH=7.8, 30°C, 150r / min, adding culture solution and bacteria solution, and the whole reaction volume was 100ml. The concentration of NH4-N is successively set to 0mg / l, 10mg / l, 20mg / l, 50mg / l, 100mg / l, 200mg / l, 500mg / l, 1000mg / l. In order to prevent the influence of acidity produced by ammonia oxidation on bacterial activity, the culture solution contained 50mmol / L phosphate buffer. The ammonia oxidation ability of the bacterial strain was judged by measuring the removal rate of ammonia nitrogen, and the results were as follows: figure 2 As shown, if the initial concentration of ammonia nitrogen is below 100mg / L, the removal efficiency of ammonia nitrogen within 24 hours is as high as 90%; even if the initial concentration of ammonia nitrogen is as high a...

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Abstract

The invention provides denitrifying strain Klebsiella sp. KSND-3 with simultaneous aerobic nitrification and denitrification performances and preparation and application thereof. The present inventionalso provides a Klebsiella strain and application thereof in river sewage and rural ammonia-containing domestic sewage. Because of fast growth rate, high cell yield and low dissolved oxygen concentration demand of the Klebsiella strain, the Klebsiella strain can be used for simultaneous nitrification and denitrification, and the denitrification process is more concise, economical and effective.

Description

technical field [0001] The invention relates to a new strain of strain Klebsiella sp. KSND-3, and the application of the strain to denitrify sewage. Background technique [0002] The rapid development of human production activities has brought more and more serious pollution to the environment. The discharge of agricultural, industrial and domestic sewage has caused serious surface water pollution, far exceeding the self-sustaining and purification capacity of the environment. A large amount of nitrogen-containing compounds in the discharged sewage can cause eutrophication of water bodies and endanger aquatic organisms and human health. Therefore, controlling the concentration of ammonia nitrogen in water body is the primary goal of environmental sewage treatment. [0003] Biological denitrification technology is currently a widely used denitrification method with high economic benefits. Traditional biological denitrification is mainly completed by the synergistic action o...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/36C12Q1/10C02F3/34C12R1/22C02F101/38
CPCC02F3/34C02F2101/38C12N1/36C12Q1/10C12N1/205C12R2001/22G01N2333/26
Inventor 郑展望张碧军金鹏谢柳
Owner ZHEJIANG SHUANGLIANG SUNDA ENVIRONMENTAL PROTECTION CO LTD
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