61 results about "Klebsiella sp" patented technology

The invention belongs to the biochemical field and discloses a 1, 3-propylene glycol genetic engineering bacterium and a preparation method and application thereof. The strain is classified and named as Klebsiella pneumoniae ATCC 25955-pUC18-yqhD-Tet<R>, and is obtained by connection of a 3-propylene redoxase isozyme gene yqhD from Escherichia coli and a tetracycline resistant gene Tet<R> from a plasmid pHY300PLK, insertion of a carrier pUC18 and conversion of Klebsiella pneumoniae ATCC 25955. The bacterium can obviously improve the capacity for conversion of glycerol into 1, 3-propylene glycol, improve the utilization ability and the conversion rate of the substrate glycerol and the final concentration of the final offspring 1, 3-propylene glycol, simultaneously shorten the fermentation time, and is convenient for industrial production of the 1, 3-propylene glycol through the microbial fermentation method.

The invention belongs to the field of environment microbiology, and particularly discloses a strain of Klebsiella sp. Bacteria CGMCC13201. The bacteria can effectively degrade crude oil of oil-containing sludge, oil-containing silt in the soil polluted by the crude oil and the like. In addition, the invention also discloses a method for degrading oil stains by using the bacteria.

The invention relates to a compound microbial preparation for degrading phenol endocrine disrupter and a preparation method, and relates to microorganism projects and sewage and waste water treatment processes. The compound microbial preparation comprises Klebsiella sp., azospirillum lipoferum, rhodococcuserythropolis and bacillus licheniformis. The microbial content of Klebsiella sp. accounts for 30%-50% of the total microbial content. The microbial content of azospirillum lipoferum accounts for 30%-50% of the total microbial content. The microbial content of rhodococcuserythropolis accounts for 10%-30% of the total microbial content. The microbial content of bacillus licheniformis accounts for 10%-30% of the total microbial content. The compound microbial preparation is fed into an anaerobic tank and an aerobic tank, and the sewage and waste water treatment processes are enhanced. The compound microbial flora can tolerate the high-concentration phenol endocrine disrupter which is used as organic matter needed in growth for being fully utilized and degraded, the sewage and waste water treatment processes are optimized, the secondary pollution of output water and sludge is reduced, and the safety of sludge landfill and agricultural utilization is improved.

The present invention discloses a strain of anti-antimony bacteria Klebsiella sp.), wherein the preservation number is CGMCC No.12896. According to the present invention, the bacteria have the high anti-antimony property, can resist a variety of heavy metals such as As<3+>, Cd<2+>, Cr<6+>, Hg<2> and the like, can promote plant growth, provide important significance for promotion of local plant growth and releasing of poisoning of heavy metals on plants, and have broad application potential in biological repair in heavy metal-polluted ore soil.

The invention relates to denitrifying phosphorus accumulation organism (DPAO) with a function of efficiently removing nitrogen and phosphorus and application of the DPAO, and belongs to the field of environmental microbiology. The bacterial strain is named Klebsiella sp. N14, belongs to Klebsiella species, and is preserved in China general microbiological culture center (CGMCC), the preservation number of the bacterial strain is CGMCC No. 10290. The bacterial strain is a Gram negative bacteria without spore and has thick capsule in a thick and short rod shape; the bacterial colony is milky white, the center of the bacteria is protruded, the edge is regular, and the surface is wet, transparent and glossy. The bacterial strain has a function of synchronously removing the nitrogen and the phosphorus, and can be applied to sewage biological sewage treatment process; after the bacterial strain is aerobically cultivated in nitrogen and phosphorus containing sewage for 24 hours, the phosphorous removal rate is 81.79%, and the nitrogen removal rate is 85.94%. The denitrifying final product of the bacteria strain is identified to be N2 with the full denitrifying capability; when the bacterial strain is used for treating the nitrogen and phosphorus containing sewage, inorganic nitrogen, such as nitrate nitrogen and nitrite nitrogen, in a water body is directly reduced to be harmless nitrogen gas to be discharged out of the water body; the effect is remarkable, the cost is low, and no secondary pollution exists.

The invention discloses medical organic silicon quaternary ammonium salt antibacterial agent modified PP and a preparation method thereof. The modified PP is prepared through the following steps: weighing the following components: PP, zinc stearate, an organic silicon quaternary ammonium salt antibacterial agent, sodium benzoate, LDPE, calcium carbonate, an antioxidant, phosphite ester, polybutadiene, barium stearate, an ultraviolet ray absorbent, calcium stearate, a styrene thermoplasticity elastomer, CPE and carbon black in parts by weight, uniformly mixing the weighed components, and extruding and pelleting the uniformly-mixed components so as to obtain the modified PP. The PP has the advantages that the impact strength is 3.5-5.5 kJ / m<2>, elasticity modulus is 2-4 GPa, the antibacterial rate for escherichia coli is 85-95%, the antibacterial rate for golden staphylococcus is 95-99%, the bacteriostasis rate for pseudomonas aeruginosa is 97.5-99.5%, the bacteriostasis rate for salmonella typhimurium and Klebsiella pneumoniae is 92-96%, and the bactericidal rate for sarcina is 90-95%.

The invention discloses a genetically engineered bacterium used for producing 3-hydroxypropionic acid (3-HP) by fermentation, a constructing method and application of the genetically engineered bacterium. An acetaldehyde dehydrogenase gene in Saccharomyces cerevisiae and a glycerol dehydratase gene in Klebsiella pneumoniae are connected onto a prokaryotic expression vector pET30a(+) so as to construct a Pet30a-ALDH-DHAB double-gene prokaryotic expression vector, and then the constructed Pet30a-ALDH-DHAB double-gene prokaryotic expression vector is transformed in to an escherichia coli BL21(DE3) competent cell to construct the genetically engineered bacterium used for effectively producing the 3-HP by fermentation. The problems of high energy consumption and sever pollution in chemical industrial synthesis of the 3-HP and the problem of low yield rate in microbial fermentation production of the 3-HP are solved. By utilizing the genetically engineered bacterium to produce the 3-HP by fermentation, a yield of the 3-HP can reach to 8.443g / L, and a production process is simple and convenient for industrialized application.

The invention discloses a medical PP inorganic antibacterial composite and a preparation method thereof. The medical PP inorganic antibacterial composite is prepared by use of the steps of weighing PP, divinyl benzene, a nano-zirconium phosphate silver-loaded antibacterial agent, an AC foaming agent, calcium stearate, PE, an antioxidant, paraffin, dicumyl peroxide, titanium dioxide, a surface treating agent, thiodipropionic acid dilauryl ester, azodicarbonamide, sodium benzoate and a titanate coupling agent in parts by weight, mixing all the components evenly and then extruding and pelletizing. The relative density of the obtained composite is 0.05-0.45 and the tensile strength of the composite is 2.5-4.5MPa; besides, the composite has the antibacterial rate of 99.5-99.9% to escherichia coli, 99.5-99.9% to staphylococcus aureus and 99-99.5% to pseudomonas aeruginosa, the bending strength of 11-15MPa, the degree of crosslinking of 45-55%, the impact strength of 3-5kJ / m<2>, and the bacteriostasis rate of 92-96% to salmonella typhimurium and Klebsiella pneumonia.

A method for remedying nitrate pollution in groundwater by adopting Klebsiella sp. DB-2 is characterized by taking soluble carbonaceous organic materials as carbon sources and the purified Klebsiella sp. DB-2 as a bioremediation strain. The method comprises in-situ remediation and ex-situ remediation. The method of the invention can be effectively used for bioremediation of the nitrate pollution in the groundwater.

The invention discloses a medical silver-carrying antibacterial agent modified PP material and a preparation method thereof. The PP material is prepared through the following steps: weighing the components of PP, EPDM, a nanometer silver-carrying zirconium phosphate antibacterial agent, barium stearate, acetone, talcum powder, an antioxidant, titanium dioxide, dicumyl peroxide, butadiene rubber, a surface treating agent, lead stearate, propylene, zinc oxide and 5-ethylidene-2-norbornene in parts by weight, uniformly mixing the weighed components, and extruding and pelleting the uniformly-mixed components so as to obtain the PP material. The PP material has the advantages that the breaking elongation rate is 15-55%, the tensile strength is 28-30 MPa, the antibacterial rate for escherichia coli is 98-99%, the antibacterial rate for golden staphylococcus is 97.5-99.5%, the impact strength is 3-5 kJ / m<2>, the bactericidal rate for pseudomonas aeruginosa is 99-99.5%, and the bacteriostasis rate for salmonella typhimurium and Klebsiella pneumoniae is 92-96%.

The invention discloses a medical antibacterial ABS material and a preparation method thereof. The ABS material is prepared through the following steps: weighing the following components: ABS, alkyl diphenyl phosphate, nanometer silver-carrying zirconium phosphate antibacterial agent, magnesium hydroxide, antibacterial copper, titanium dioxide, a coupling agent, magnesium stearate, dicumyl peroxide, SBS, a surface treating agent, graphite, EVA, micro-capsulated red phosphorus and dimethyl polysiloxane in parts by weight, uniformly mixing the weighed components, and extruding and pelleting the uniformly-mixed components so as to obtain the ABS material. The medical antibacterial ABS material has the advantages that the heat deformation temperature is 85-105 DEG C, the tensile strength is 40-60 MPa, the antibacterial rate for escherichia coli is 95-99%, the antibacterial rate for golden staphylococcus is 97.5-99.5%, the impact strength is 0.2-0.6 kJ / m, the bacteriostasis rate for pseudomonas aeruginosa is 97-99%, and the bacteriostasis rate for salmonella typhimurium and Klebsiella pneumoniae is 91-95%.

The invention relates to a Klebsiella sp. and its application. The Klebsiella sp. DHU-S1 has the preservation number of CGMCCC No. 18075, and its nucleotide sequence is as shown in SEQ ID NO. 1. The Klebsiella sp. is used for dissolving and killing blue-green algae in a water body. The most suitable algae-dissolving conditions of the strain are as follows: inoculation amount is 10%, pH is 7.0, temperature is 25 DEG C, and 10g / L glucose is added as a supplementary carbon source. Under the conditions, the dissolution rate of blue-green algae by the Klebsiella sp. reaches 100%.

The invention discloses a high-throughput micro-fluidic LAMP chip for detecting livestock respiratory tract pathogens and a detection method, and belongs to the technical field of biochips. The LAMP chip comprises an LAMP amplification reagent, a DNA template, an LAMP quality control positive control, an LAMP quality control negative control, a detection chip, a chip probe and a microfluidic LAMPchip analyzer, wherein the detection chip is arranged in the isothermal amplification microfluidic LAMP chip analyzer; and the chip probe comprises nucleotide sequences as shown in SEQ ID NO: 1-23. The LAMP chip is used for detecting specific nucleic acid fragments of mannheimia haemolytica, pasteurella multocida, mycoplasma, arcanobacterium pyogenes and klebsiella pneumoniae. The LAMP chip provided by the invention can complete detection within 50 minutes, has a detection limit of 1.5x10<-2> ng / mu L, can monitor main livestock respiratory tract pathogens in real time, has the characteristicsof simple operation, rapid detection, high sensitivity and strong specificity, and brings convenience to detection of the livestock respiratory tract pathogens.

The invention discloses a Klebsiella sp. strain, a pyridine biodegradation agent as well as a preparation method and an application of the pyridine biodegradation agent. The Klebsiella sp. strain is Klebsiella sp. BD2, is preserved in China Center for Type Culture Collection with preservation number of CCTCC NO: M 2018685. The pyridine biodegradation agent is prepared from the Klebsiella sp. strain by activation and culture. The Klebsiella sp. BD2 pyridine has high degradation efficiency, good salt tolerance and good environmental tolerance, and the pyridine biodegradation agent prepared fromKlebsiella sp. BD2 can be applied to degradation of pyridine in wastewater and soil, and has high degradation efficiency.

The invention relates to a high efficiency chlopyrifos degradating strain Klebsiella sp. CPK capable of generating magic spots, and the accession number of the strain is CGMCC No.4167. The invention relates a preparation method and application of the strain. The strain provided in the invention can be widely used for removal of residual pollution of chlopyrifos and for bioremediation and has the advantages of powerful degradation capability, stable degradability, in-depth metabolic studies, etc.

The invention relates to a 4,5-glyoxalidine [1,2-a] quinoline derivative shown as the following formula (1) and application of the 4,5- glyoxalidine [1,2-a] quinoline derivative, wherein R represents C1-C12 alkyls, benzyl and substituted benzyl, Y represents H, O and N, and R1 represents C1-C12 alkyls, phenyl, substituted phenyl, benzyl and substituted benzyl. The invention also provides a medicine composition containing the derivative and salts of the derivative, and application of the derivative and the salts of the derivative to antibacterial medicine preparation. The derivative and the salts of the derivative respectively have the inhibition effects on staphylococcus aureus, escherichia coli, other gram-negative bacteria (such as pseudomonas aeruginosa, Klebsiella pneumoniae, proteusbacillus vulgaris, salmonella and pasteurella) and gram-positive bacteria, and in other aspects, the antibacterial effect is better than that of ciprofloxacin and amoxicillin.

The present invention relates to: a novel Klebsiella sp. IR21 or Candida sp. IR11 strain; a composition for wastewater treatment or wastewater purification, containing the same; a method for treating or purifying wastewater by using the composition; a microbial fuel cell containing the strain; a method for producing electricity by using the microbial fuel cell; a wastewater treatment apparatus comprising the microbial fuel cell; and a method for treating or purifying wastewater by using the wastewater treatment apparatus. The novel Klebsiella sp. IR21 or Candida sp. IR11 strain of the present invention allows the purification of wastewater exhibiting a remarkably high COD (Chemical Oxygen Demand) value and the production of electricity to be simultaneously promoted and allows a wastewater treatment apparatus to be operated without an external power source by designing a wastewater treatment apparatus comprising a microbial fuel cell, and thus could be widely applied to more effective wastewater treatment.

The invention provides lipase CALB mutant and a preparation method and application thereof. An error-prone PCR technology in a gene in-vitro directed evolution strategy is utilized, mutagenesis in-vitro is conducted on a wild type lipase gene from Klebsiella sp., screening is conducted by distinguishing a culture medium, and a strain for efficiently producing lipase is obtained. The obtained lipase CALB mutant is high in expression activity, the expression activity is 8.7 times catalytic activity of the wild type lipase, and the lipase CALB mutant can be used for industries related to lipid hydrolysis. Batched production of the lipase CALB mutant can be achieved through large-scale fermentation of engineering bacteria containing the gene for encoding the CALB mutant.

The invention relates to a triple fluorogenic quantitative PCR detection method of escherichia coli, Klebsiella pneumoniae and acinetobacter, and belongs to the technical field of fluorogenic quantitative PCR detection. The detection method comprises the following steps: preparing a 25ul reaction system by 10ul nucleic acid of a sample and a 15ul reaction liquid, and then carrying out fluorogenic quantitative PCR reaction; and determining that the sample contains escherichia coli if an S-shaped amplification curve is existent in the FAM detection channel and Ct value is less than 30, determining that the sample contains Klebsiella pneumoniae if the S-shaped amplification curve is existent in the HEX or VIC detection channel and Ct value is less than 30, and determining that the sample contains acinetobacter if the S-shaped amplification curve is existent in CY5 detection channel and Ct value is less than 30. The whole detection process is less than two hours, a closed-tube reaction is adopted, and the reaction system is processed directly after reaction, so the aerosol pollution caused by opening is avoided. The process is simple and the detection time is short.

The invention belongs to the technical field of microbial agents, and particularly relates to a compound bacterium preparation capable of efficiently adsorbing mercury and application of the compound bacterium preparation. The preparation of the compound bacterium preparation comprises the following steps: (1) performing separation and purification in mercury-polluted soil to obtain Klebsiella pneumoniae and Bacillus toyonensis, and performing separation and purification in a microbial selenium-rich microbial agent to obtain Bacillus subtilis, wherein the separation and purification are carried out through a mercury-containing culture medium; and (2) performing enrichment culture on the Klebsiella pneumoniae, the Bacillus toyonensis and the Bacillus subtilis obtained in the step (1), performing mixing according to an enrichment liquid mass ratio of (0.8 to 1.2):(0.8 to 1.2):(0.8 to 1.2), and inoculating the mixed strains into a culture medium for culturing to obtain a culture solution which is the compound bacterium preparation. By using the adsorption performance of the composite bacterium preparation on the effective mercury, the mercury is prevented and controlled from migrating from soil to plants, three kinds of microbes in the compound bacterium preparation achieve a synergistic effect, efficient adsorption on mercury is realized, and the adsorption rate is obviously superior to that of a single strain.

The invention provides a genetically engineered bacterium for co-production of 1, 3-propylene glycol and polyhydroxybutyrate as well as a construction method and application thereof, and belongs to the technical field of genetic engineering. The genetically engineered bacterium is classified and named as Klebsiella pneumoniae Q3-PHB, and the preservation registration number of the genetically engineered bacterium is CCTCC M 20191090. The construction method of the strain is simple; a batch feeding fermentation method is adopted; finally, the biomass of the thalli is greatly increased; the yield of the 1, 3-propylene glycol reaches 76.21 g / L; the conversion rate of 1, 3-propylene glycol is 0.56 mol / mol, the contents of ethanol and lactic acid are effectively controlled to below 1g / L; meanwhile, the thallus accumulation PHB accounts for 42.36% of the dry weight of thallus, so that the production cost is reduced, the downstream separation cost is greatly reduced, the comprehensive economic benefit is relatively high, and the industrial application value is achieved.

The invention discloses a fusion protein modified by klebsiella pneumonia polysaccharide, and application of the fusion protein. A preparation method for the fusion protein modified by the klebsiellapneumonia polysaccharide comprises the step of expressing the encoding gene of the protein in creatures to obtain the protein modified by the klebsiella pneumonia polysaccharide. The protein is a) orb) or c) or d), wherein a) is the protein formed by an amino acid sequence of SEQ ID No.3, b is the protein formed by an amino acid sequence of SEQ ID No.5, c) is the fusion protein obtained by carrying out protein tag fusion on the carboxyl terminal or / and amino terminal of the protein disclosed by a) or b), and d) is the protein obtained by carrying out substitution and / or deletion and / or addition on the amino acid sequence disclosed by a) or b) through one or multiple amino acid residues.

The invention discloses an anti-drug-resistance antibacterial amidine oligomer, a preparation method, application and an antibacterial mechanism of the antibacterial amidine oligomer, and an application of the antibacterial amidine oligomer in sterilization in an intracellular infection model and a zebra fish model infected by mycobacterium fortuitum. The antibacterial types of the amidine oligomer comprise escherichia coli, enterococcus faecalis, staphylococcus aureus, klebsiella pneumoniae, acinetobacter baumannii, pseudomonas aeruginosa, mycobacterium smegmatis, mycobacterium fortuitum and the like. The structure disclosed by the invention has double antibacterial mechanisms of destroying bacterial cell membranes and combining chromosome DNA, has the characteristics of rapid sterilization and drug resistance, and shows antibacterial broad spectrum. In addition, the structure has low toxicity to blood cells, and has high safety in blood. Meanwhile, the structure can be applied to a Raw264.7 cell model infected by mycobacterium smegmatis and mycobacterium fortuitum, and the structure also has a good treatment effect on the zebra fish model infected by MRSA and mycobacterium fortuitum.

The invention discloses an antibacterial amidine oligomer with resistance to drug resistance, a preparation method, application and an antibacterial mechanism of the antibacterial amidine oligomer, and application of the antibacterial amidine oligomer in sterilization in an intracellular infection model and a zebra fish model infected by accidental mycobacteria. The antibacterial types of the amidine oligomer comprise escherichia coli, enterococcus faecalis, staphylococcus aureus, klebsiella pneumoniae, acinetobacter baumannii, pseudomonas aeruginosa, mycobacterium smegmatis, mycobacterium fortuitum and the like. The structure disclosed by the invention has double antibacterial mechanisms of destroying bacterial cell membranes and combining chromosome DNA, has the characteristics of rapid sterilization and drug resistance, and shows antibacterial broad spectrum. In addition, the structure has low toxicity to blood cells, and has high safety in blood. Meanwhile, the structure can be applied to a Raw264.7 cell model infected by mycobacterium smegmatis and mycobacterium fortuitum, and the structure also has a good treatment effect on a zebra fish model infected by MRSA and mycobacterium fortuitum.

The invention discloses a lasting bacteriostatic laundry detergent and a preparation method thereof. The lasting bacteriostatic laundry detergent is prepared from the following raw materials in percentage by mass: 5 to 20 percent of anionic surfactant, 5 to 20 percent of nonionic surfactant, 0.5 to 10 percent of fatty acid, 0.1 to 5 percent of bacteriostatic agent, 0.1 to 5 percent of citric acid monohydrate, 1 to 10 percent of alkali, 0.1 to 5 percent of modified acrylic polymer, 0.05 to 0.5 percent of protease, 0.05 to 0.5 percent of preservative, 0.05 to 0.5 percent of essence, 0.05 to 0.5 percent of sodium chloride and the balance of deionized water. The laundry detergent has an efficient and rapid antibacterial effect and can be used for rapidly removing escherichia coli and staphylococcus aureus; the laundry detergent has a good lasting bacteriostatic effect, can achieve 72-hour lasting bacteriostasis on escherichia coli, staphylococcus aureus and klebsiella pneumoniae, and can effectively inhibit bacterial reproduction, prevent peculiar smell and maintain long-acting freshness of fabrics for clothes aired indoors or dried in the shade in a humid environment.

The invention discloses an inactivated germ melt-blown fabric. The inactivated germ melt-blown fabric comprises the following components in parts by weight of 60-150 parts of polypropylene PP-1500, 1-9 parts of zinc oxide and 2-18 parts of cuprous oxide; the invention also discloses a production process of the inactivated germ melt-blown fabric, which comprises the following steps of S1, raw material pre-preparation treatment: preparing 80kg of polypropylene PP-1500, 2.5 kg of zinc oxide and 4.5 kg of cuprous oxide, uniformly putting the raw materials into a stirring barrel, and stirring for 40-80 minutes for later use, and S2, melting and spraying: heating and plasticizing the standby raw materials through a main screw machine, then accurately quantifying through a metering pump, and spraying a melt onto a mesh belt receiver through a melt-blowing nozzle. Zinc oxide and cuprous oxide in the non-woven fabric have a good sterilization effect on escherichia coli, klebsiella pneumoniae and staphylococcus aureus, and the sterilization effect is far beyond the existing sterilization standard.

The invention discloses a synechocystis PCC6803 strain for producing alkaline pectate lyase and a construction method of the synechocystis PCC6803 strain, and belongs to the field of industrial microorganisms. An alkaline pectate lyase gene petyl derived from Klebsiella sp.Y1 is recombined into a synechocystis PCC6803 genome through a homologous recombination method, and a synechocystis PCC6803 genetic engineering strain PSY1 for producing the alkaline pectate lyase petyl is constructed, so that a method for producing pectinase by utilizing photosynthetic organisms is realized, a new way for producing the pectinase by using inorganic salt and carbon dioxide is obtained, and the consumption of energy (mainly carbon source) in a biomass conversion process is reduced.

The invention discloses a rhizosphere probiotic Klebsiella sp ZH07. The rhizosphere probiotic Klebsiella sp ZH07 is a microorganism which is separated from peanut soil which is continuously planted for ten years and can degrade peanut autotoxic substances. The rhizosphere probiotic Klebsiella sp ZH07 has a degradation effect on the peanut autotoxic phenolic acid substances including benzoic acid,p-hydroxybenzoic acid, salicylic acid, cinnamic acid, coumaric acid and vanillic acid, and the degradation rates of the six autotoxic substances all exceed 50%; after the rhizosphere probiotic Klebsiella sp ZH07 is cultured by adopting a bacterium solution containing the rhizosphere probiotic Klebsiella sp ZH07, the dry weight of peanuts is increased by about 32%, the height of seedlings on the ground is increased by about 32%, and the root length of roots under the ground is increased by about 6%; and the rhizosphere probiotic Klebsiella sp ZH07 has an obvious growth promoting effect on the peanuts and can promote growth of peanut plants.