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Anti-antimony bacteria NXH2 and applications thereof

A technology of bacteria gram and lebsiella, applied in the field of environmental biology, can solve problems such as affecting the growth of crops, polluting the farmland in mining areas, reducing the quality of crops, etc. Effect

Active Publication Date: 2016-12-21
北京优生基生态科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At the same time, the mining and smelting activities of antimony mines will also cause heavy metal elements associated with antimony, such as arsenic and mercury, to enter the supergene environment of the mining area, pollute the farmland in the mining area, not only affect the growth of crops, but also reduce the quality of crops

Method used

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  • Anti-antimony bacteria NXH2 and applications thereof
  • Anti-antimony bacteria NXH2 and applications thereof
  • Anti-antimony bacteria NXH2 and applications thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Example 1 Isolation and screening of antimony-resistant bacteria and anti-Sb ability

[0016] Collect plant rhizosphere soil samples near the smeltery in Xikuangshan Antimony Mining Area, Lengshuijiang City, Hunan Province, weigh 100g of the above-mentioned fresh soil samples, add an appropriate amount of antimony potassium tartrate ([C 8 h 4 K 2 o 12 Sb 2 3(H 2 O)]), so that the final concentration of antimony in the soil sample was 1000mg / kg, and placed in a constant temperature incubator at 28°C for enrichment and cultivation for one week. Take 10g of the above soil sample and place it in a sterile solution containing 10 glass beads and 90mL of 0.85% NaCl, shake it in a shaker at 30°C and 180rmin-1 for 30min to fully disperse the sample. Take 0.1mL and spread it on the CDM medium (CDM medium formula: MgSO 4 ·7H 2 O 2.0 g, NH 4 Cl 1.0 g, Na 2 SO 4 1.0g, K 2 HPO 4 0.013 g, CaCl 2 2H 2 O 0.067 g, Na-lactate 5.0 g, agar 15.0 g, add distilled water to 1000...

Embodiment 2

[0019] The identification of embodiment 2 NXH2 bacterial strain

[0020]The strain was subjected to morphological, culture characteristics and sequence analysis of 16S rDNA. The 16S rDNA molecular identification was carried out according to the following steps: pick a single colony of the screened strain and inoculate it in liquid LB medium, culture it on a shaker at 30°C and 150r / min, take out the culture medium at 24h, and centrifuge at 5000r / min for 1min to get the supernatant According to the bacterial genomic DNA extraction kit (provided by Tiangen Biochemical Technology Co., Ltd.), the colony DNA was extracted; the general primers 27F and 1492R were used for PCR amplification of the extracted bacterial DNA; the sequence of 27F was 5′-AGA GTT TGA TCC TGG CTC AG -3'; 1492R sequence is 5'-AAGGAG GTG ATC CAG CCG CA-3' The primer was synthesized by Beijing Ruibo Xingke Biotechnology Co., Ltd.; the PCR product was sequenced, and the sequencing results were analyzed by BLAST in...

Embodiment 3

[0022] Example 3: Tolerance of Klebsiella NXH2 to various heavy metals

[0023] Separately prepared with different concentrations of As 3+ 、Cd 2+ 、Cr 6+ , Hg 2 CDM solid medium for heavy metals, Cd 2+ 、Cr 6+ , Hg 2 Heavy metal concentration starts from 100mg / L, followed by 100mg / L, 200mg / L, 400mg / L, 800mg / L, 1200mg / L, 1600mg / L, As 3+ Concentration starting from 100mg / L, followed by 100mg / L, 200mg / L, 400mg / L, 800mg / L, 1200mg / L, 1600mg / L, 2000mg / L, 3000 mg / L, 4000 mg / L, without adding Heavy metal treatment served as a control. Klebsiella sp. ( Klebsiella sp. ) NXH2 strain was inoculated into heavy metal-free CDM medium for activation and culture for 24 hours, and 0.1 mL was inoculated into medium containing different types and concentrations of heavy metals, and cultured at 30°C for 24 hours. , The growth situation in the culture medium of different concentrations of heavy metals, the results are shown in Table 1. It can be seen from the table that the NXH2 strain has...

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Abstract

The present invention discloses a strain of anti-antimony bacteria Klebsiella sp.), wherein the preservation number is CGMCC No.12896. According to the present invention, the bacteria have the high anti-antimony property, can resist a variety of heavy metals such as As<3+>, Cd<2+>, Cr<6+>, Hg<2> and the like, can promote plant growth, provide important significance for promotion of local plant growth and releasing of poisoning of heavy metals on plants, and have broad application potential in biological repair in heavy metal-polluted ore soil.

Description

technical field [0001] The invention belongs to the technical field of environmental biology, and in particular relates to an antimony-resistant bacterium NXH2 and its application. Background technique [0002] In recent years, with the continuous expansion of the scale of antimony ore mining and the discharge of waste in the antimony industry, the soil around the mining area has been seriously polluted. As environmental pollutants, antimony and antimonides have been proven to be a class of genotoxic substances and carcinogenic to humans (Huang et al., 1998; Takahashi et al., 2002; Beyersmann et al., 2008). In addition, antimony can also cause lung injury, hematuria, fibroblast death, sister chromatid exchange, circulatory system diseases, etc. (Huang et al., 1998). Antimony has been listed as a priority pollutant by the US Environmental Protection Agency and the Council of the European Community, and it is also a pollutant closely monitored by the Japanese Environmental Ag...

Claims

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Application Information

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IPC IPC(8): C12N1/20B09C1/10A01N63/00A01P21/00C12R1/22
CPCA01N63/00B09C1/10C12N1/205C12R2001/22
Inventor 彭霞薇聂孝红尹昊王尔纯徐鸣
Owner 北京优生基生态科技有限公司
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