33 results about "Molecular identification" patented technology

The present application belongs to the technical field of molecular identification of new shrimp species, and particularly relates to a composition for identifying Neocaridina denticulata and Neocaridina zhoui and use thereof. The present application discloses a composition for identifying Neocaridina denticulata and Neocaridina zhoui, which comprises: a first specific primer pair with a nucleotide sequence as shown in SEQ ID NO: 3 and SEQ ID NO: 4; and a second specific primer pair with a nucleotide sequence as shown in SEQ ID NO: 5 and SEQ ID NO: 6. Based on the composition and the corresponding duplex PCR method provided by the present application, a 743 bp band specific to Neocaridina denticulata or a 601 bp band specific to Neocaridina zhoui can be amplified simultaneously in one reaction system, so that the two morphologically similar new shrimp species can be identified quickly, accurately and efficiently.

The application discloses a Cmfsg gene mutant of melon and a creation method and application thereof, and belongs to the technical field of plant genetic engineering. The mutant is obtained by CRISPR / Cas9 gene editing technology to direct mutation of the coding region or the promoter region of the Cmfsg gene of melon, the target point is recognized by a specific sgRNA primer, and is screened by specific primer PCR amplification and sequencing verification. The creation method comprises the following steps: constructing an editing vector of a target gene, introducing the wild-type queen melon by means of an agrobacterium-mediated genetic transformation technology, screening, regeneration and molecular identification to obtain the mutant. The Cmfsg gene has a regulation function on the fruit fur character and the fruit shape index of melon, can realize the direct and rapid creation of the appearance character of melon, overcomes the defects of long breeding period and low efficiency in traditional breeding, and the obtained mutant has stable genetic traits, and can be used as a parent or genetic resource for high-quality breeding of melon.

The present application provides a DNA barcode specific identification method of radix rehmanniae decoction pieces, and relates to the technical field of true and false identification of traditional Chinese medicine decoction pieces. The specific identification method comprises the following steps: extracting sample DNA to be detected; screening universal and specific DNA barcode genes; amplifying universal and specific DNA barcode gene fragments; amplifying the amplification product; and obtaining amplified DNA barcode sequences after sequence correction and splicing, and performing online BLAST comparison with NCBI database, so as to determine the base origin of the sample to be detected. The method provided by the present application can overcome the problem that the DNA barcode of the conventional ITS2 sequence of the decoction piece after processing is not easy to amplify, and the PCR amplification product of the present application has high quality, the molecular identification result is accurate, and the rapid identification of radix rehmanniae and radix rehmanniae preparata decoction pieces can be efficiently and high-quality solved, and the urgent need for true and false identification of radix rehmanniae decoction pieces during acceptance is solved.

The invention discloses trichoderma sp. Y3 and application thereof in promoting rice growth, and belongs to the technical field of microorganisms. The classification name of the trichoderma sp. Y3 is Trichoderma sp. Y3, the trichoderma sp. Y3 is preserved in the China Center for Type Culture Collection, and the preservation number is CCTCC NO: M 2026214. And biological characteristics and molecular identification are combined to identify that the trichoderma Y3 belongs to a new trichoderma species. When the strain or a fermentation product thereof is applied to a rice culture medium, the growth of rice can be effectively promoted, and the plant biomass is increased. Trichoderma Y3 is developed and prepared into the biological bacterial fertilizer, and a new way is provided for application of microorganisms to development of crop production.

This invention relates to the field of molecular biology, specifically disclosing a molecular identification code for the genus *Aristolochia*, its combination, and its applications. The molecular identification code for *Aristolochia* of this invention is the nucleotide sequence shown in SEQ ID No. 1. The molecular identification code combination for *Aristolochia* is the nucleotide sequence shown in SEQ ID No. 1-6. This invention can determine the presence of *Aristolochia* species in a test sample by detecting whether the PCR amplification product or raw high-throughput sequencing data contains the nucleotide sequence shown in SEQ ID No. 1 or SEQ ID No. 1-6. This invention can accurately identify *Aristolochia* species, has broader applicability, and can detect the presence of *Aristolochia* species in all samples from which DNA can be extracted. It enables rapid and accurate identification of original plants, medicinal materials, powders, traditional Chinese medicine preparations, and mixed food materials of the *Aristolochia* genus.

This invention discloses a method for regulating cucumber seed germination at low temperatures. CsGAI Mutated genes and their applications. This invention constructs a targeted gene... CsGAI The gene editing vector pCas9CsGAI was used to transform cucumbers, and the resulting plasmid was then transformed into cucumbers. CsGAI Genetically modified plants were used for field hybridization and pollination, molecular identification, and phenotypic identification to prove that when CsGAI CsGAI CsGAI CsGAI CsGAI CsG When gene function is lost or weakened, the relative germination rate, relative germination potential, and relative germination index of cucumbers increase, and their cold tolerance improves. This invention lays the foundation for revealing the genetic and biological mechanisms of cold tolerance during cucumber germination, provides new gene resources for the cultivation of cold-tolerant cucumbers, and is of great significance for cucumber cold research and the breeding of new cold-tolerant varieties.

The application discloses a method for identifying authenticity of Cordyceps sinensis and products thereof based on fluorescent duplex PCR, and relates to the technical field of molecular identification of traditional Chinese medicinal materials. The method comprises the following steps: extracting total nucleic acid from a sample to be tested; using a fungal specific primer pair and an insect specific primer pair in the same PCR reaction tube, performing duplex PCR amplification by means of SYBR Green dye method, and collecting a fluorescent signal, the fungal specific primer pair is targeted at an ITS sequence of Cordyceps sinensis, and the insect specific primer pair is targeted at a COI gene sequence of host Thaumastia bennetti larvae; and determining results according to amplification curves and melting curves. The application provides a primer combination which can realize balanced and specific synchronous amplification of two target points at a universal annealing temperature of 60 DEG C, and breaks through the bottleneck of compatibility of duplex fluorescent PCR amplification caused by the conflict between the high GC content of the fungal ITS region and the low GC content of the insect COI region. The method is closed tube operation throughout, does not need gel electrophoresis and fluorescent probes, and has the advantages of high accuracy, convenient operation and low cost, and can be widely applied to authenticity identification of Cordyceps sinensis and deep processing products thereof.

The application discloses a kind of single-cell transcriptome analysis methods and application of Crohn's disease creeping fat microenvironment, belong to the field of biological medicine technology.The method is by obtaining the single-cell and batch transcriptome data of mesenteric adipose tissue of Crohn's disease patient and healthy control, constructs relevant gene set and carries out data quality control, clustering and cell annotation;Mesenchymal stem cells are subpopulation identified, abundance analysis and function typing, combined with signal path analysis and cell communication analysis, and the key factor of regulating creeping fat microenvironment is screened out.The application first systematically analyzes the cell heterogeneity and MSC subpopulation characteristics of Crohn's disease creeping fat microenvironment, and determines the key signal path and regulating molecule, which can provide efficient and reliable technical means for the pathogenesis research, treatment target screening and creeping fat related molecule identification of Crohn's disease, and has high scientific research and clinical application value.

Methods for correcting consensus sequence reads are described. The disclosed methods can comprise: obtaining a sample of double stranded nucleic acid molecules having jagged ends; performing end repair on the jagged ends to introduce jagged end identifiers; preparing a sequencing library comprising the end repaired nucleic acid molecules; sequencing the library to generate sequence reads; mapping the sequence reads to a reference genome to generate a plurality of mapped sequence reads and corresponding sequence information, where the corresponding sequence information includes a jagged end position based on the jagged end identifier, a start position, and a stop position of a given mapped sequence read; grouping the mapped sequence reads into families based on the corresponding sequence information; determining a consensus sequence read for each family; and detecting a biological signal in the consensus sequence read for at least one family of a plurality of families.

The application discloses a method for delaying leaf senescence of Brassica chinensis var. rosularis by editing genes SGR1 and SGR2, and belongs to the technical field of crop breeding. The method comprises the following steps: designing specific sgRNA according to the coding region sequence of the stay-green genes SGR1 and SGR2 of the Brassica chinensis var. rosularis, constructing a CRISPR / Cas9 gene editing vector, transforming the Brassica chinensis var. rosularis receptor material by means of Agrobacterium tumefaciens infection of the cotyledon and hypocotyl, obtaining the transformed plant containing the gene editing element through adventitious bud regeneration and screening, and screening the plant with the SGR1 and / or SGR2 gene site-directed mutation through molecular identification and phenotype observation. The application precisely modifies the stay-green genes by the gene editing technology, significantly delays the leaf senescence process of the Brassica chinensis var. rosularis, improves the leaf stay-green property and the preservation period, and keeps excellent agronomic traits, thereby providing a new way for the genetic improvement and preservation technology development of the Brassica chinensis var. rosularis, and having important application value in the fields of vegetable production, preservation and breeding.

The present application relates to pig breed molecular identification, and discloses a SNP molecular marker combination for Wannan flower pig identification, the combination is composed of the following 11 SNP sites, the physical position and allele information of each SNP site is determined based on pig reference genome Sscrofa11.1: rs342792776, located at 103395244bp of chromosome 4, the allele thereof is A / G; rs343665183, located at 104083270bp of chromosome 4, the allele thereof is G / A; novel_WNS1, located at 5240118bp of chromosome 7, the allele thereof is G / A.The present application is based on whole genome resequencing and population genetics analysis, 11 Wannan flower pig specific SNP sites are screened out, and unified REF / ALT unification, 0 / 1 / 2 numerical coding, standardization processing and threshold determination rules are combined, so that the detection process is clear, the repeatability is strong, and the standardized implementation between different detection platforms and laboratories is facilitated.

The application provides a Beauveria bassiana strain Bb-YY for preventing and controlling various diseases and pests, a derivative product and application thereof, and belongs to the technical field of functional microorganisms. Beauveria bassiana The strain Bb-YY is screened from the rhizosphere soil of a peanut healthy plant planted in a serious continuous cropping obstacle plot, and is identified as Beauveria bassiana (Bb) by molecular identification, has high efficient prevention and control effects on white grubs, peanut fruit rot pathogens and peanut root rot pathogens, and has certain inhibition effects on Bacterospora coriacea causing tobacco black foot disease and Pyricularia oryzae causing rice blast. It can be seen that the Bb-YY strain can also play a prevention and control effect on tobacco, rice and other crops in addition to peanut planting, and can effectively reduce the use of chemical pesticides, and provides a strong support for green prevention and control of peanut diseases and pests.

The application discloses a nucleic acid molecule, a vector, a kit and an application for efficient genetic transformation of a pepper. Compared with a traditional screening agent, the application can significantly improve a transgenic positive rate, greatly reduce a false positive rate, and reduce subsequent molecular identification workload. The application realizes multi-dimensional visual precise screening, can perform double screening under different tissue stages and different observation conditions, effectively eliminates background interference and gene silencing risk, and improves screening accuracy. In addition, the application breaks through a bud elongation bottleneck, effectively solves a key problem of "bud not elongation" in pepper regeneration by optimizing an exogenous additive in a bud elongation stage. In a word, the application realizes efficient regeneration from an explant to a complete plant through systematic optimization of genotype screening, bud induction, bud elongation and rooting.

The present application relates to the field of forensic pathology, forensic genetics and molecular identification technology, in particular to a primer composition for evaluating genetic susceptibility of unexplained sudden death, products and applications thereof. The primer composition provided by the present application comprises nucleotide sequences such as amplification primers shown in SEQ ID NO. 1-512, that is, 256 pairs of primers related to 238 quantitative trait loci. The present application further provides products and methods for evaluating genetic susceptibility of unexplained sudden death of individuals. Experimental results show that the primer composition or kit provided by the present application can detect consistent and highly repeatable genotyping results, and on this basis, the genetic susceptibility of unexplained sudden death can be further evaluated by calculating the polygenic risk score, which has important significance for the genetic background research of unexplained sudden death and the accurate cause of death identification in sudden death related cases in forensic identification work.

The present application belongs to the field of microorganism and biological control technology, and particularly relates to a volatile bacteriostatic substance producing Paenibacillus polymyxa LC36 and application thereof. The strain is isolated from the rhizosphere soil of Codonopsis pilosula, and is identified as Paenibacillus polymyxa through morphological, physiological and biochemical and molecular identification. Research shows that Paenibacillus polymyxa LC36 can produce cellulase, protease and IAA and other various biocontrol related substances, and release various volatile organic compounds (VOCs), among which benzyl alcohol, guaiacol, 2-phenylethanol and the like show significant inhibitory effect on the pathogenic fungus of Codonopsis pilosula, Fusarium oxysporum. The results of pot experiment show that the strain has a control effect of 57.6% and 87.8% on the root rot and wilt of Codonopsis pilosula, respectively, and shows good biocontrol application prospect. The present application provides a new microbial resource and technical support for the green prevention and control of Codonopsis pilosula soil-borne diseases.

This invention relates to a molecular marker, specific primers, and their applications for identifying the brown spine trait in cucumbers. The molecular marker is located between nucleotides 853 and 854 of the cucumber gene CsBS (Sequence 1 in the sequence listing), and its nucleotide sequence is the insertion or deletion sequence shown in Sequence 2 of the sequence listing. By determining the genotype of this molecular marker, the color of the cucumber spines can be identified as brown. This molecular marker originates from the functional variation controlling the trait itself, co-segregates with the phenotype, and the identification results are consistent with the field spine color phenotype, with an accuracy approaching 100%. The molecular marker and specific primers can be used for the molecular identification of the brown spine trait in cucumbers and for assisted breeding of brown-spined cucumbers. This enables early, non-destructive selection of the target brown spine trait without waiting for the plant to flower and bear fruit, significantly shortening the breeding cycle and saving manpower, material resources, and land resources.

This invention discloses a systematic preparation method for a soil matrix specifically suitable for slope ecological restoration. The soil matrix mainly consists of a highly efficient soil microbial agent and a scientifically proportioned basic soil matrix. The method specifically includes four key steps: (1) targeted preparation and activity assurance of the soil microbial agent; (2) precise molecular identification of functional strains and verification of key functional genes; (3) determination of the optimal application and inoculation strategy of the soil microbial agent through rigorous comparative experiments; and (4) final large-scale preparation and homogenization treatment of the soil matrix. The soil matrix prepared by this invention can significantly reduce the dependence of agricultural production on chemical fertilizers, effectively overcome the limitations of non-nitrogen-fixing plants in slope restoration, and significantly improve vegetation species diversity and ecosystem stability during slope ecological restoration.

This invention provides a method for rapid propagation of superior grass carp families, belonging to the field of aquaculture and fish genetic breeding technology. The method includes: screening for disease-resistant superior families from established grass carp families; isolating and enriching reproductive stem cells from these superior families; transplanting the grass carp family reproductive stem cells into the abdominal cavity or genital ridge region of recipient fish with short sexual maturity cycles; cultivating the recipient fish to sexual maturity, producing sperm, and then fertilizing the sperm with grass carp eggs to obtain F1 generation offspring; performing molecular identification on the offspring to confirm that their genetic material originates from the donor family. This invention can shorten the propagation cycle of superior grass carp families from 4-5 years to approximately 1.5 years, achieving precise and efficient transfer of superior traits, and providing an effective technical means to shorten the grass carp breeding cycle and cultivate superior grass carp varieties.

The invention relates to a multispectral plasmonic sensor for chiral molecular barcoding, employing a pixelated array of nanostructured plasmonic units coupled with a photonic cavity. Each pixel is tuned to resonate at distinct mid-infrared (mid-IR) wavelengths, facilitating simultaneous detection of molecular chiral and vibrational properties. The system generates near-perfect resonant absorption, maximizing electric and magnetic field enhancements. When illuminated with circularly polarized light, the sensor produces a chiral near-field with handedness determined by the polarization direction. The interaction between the sensor and chiral molecules is quantified using dissymmetry factors, forming unique chiral barcodes that encode molecular behavior across multiple wavelengths. The sensor enables surface-enhanced infrared absorption (SEIRA) induced surface-enhanced vibrational circular dichroism (SEVCD) measurements, providing comprehensive chiral and vibrational analysis. This approach creates a unique barcode for every chiral active molecule, supporting advanced applications in chemical analysis and molecular identification.

The present application belongs to the technical field of mycotoxin biodegradation and bacillus velezensis, and particularly relates to a bacillus velezensis strain with degrading vomitoxin and multiple enzyme activities and application thereof. The strain is isolated from pig intestinal contents, and is identified by morphological identification, physiological and biochemical identification, and 16S rRNA and gyrB gene molecular identification, and is confirmed as bacillus velezensis, and is named as bacillus velezensis YK-19 (Bacillus velezensis YK-19). The strain has a degrading rate of vomitoxin (deoxynivalenol, DON) as high as 93.79%, and has multiple enzyme activities such as protease, amylase, beta-mannanase, xylanase, cellulase and pectinase, and does not have hemolytic activity, and has good biological safety. The strain also shows good acid resistance, bile salt resistance, salt resistance and high temperature resistance, and has a wide application prospect in actual production.

Provided are Lagenidium juracyae with a Bursaphelenchus xylophilus (B. xylophilus)-killing activity and a use of the Lagenidium juracyae in control of B. xylophilus. The Lagenidium juracyae has a strain number of NL03 and an accession number of CGMCC NO. 40587. In the present application, an oomycete Lagenidium sp. NL03 with a B. xylophilus-killing ability is isolated from dead soil nematodes. The biological and molecular identification and the biocontrol potential investigation are conducted for Lagenidium sp. NL03 to provide a reference for the expansion of a biological control resource pool for B. xylophilus and the eco-friendly control of pine wilt disease (PWD).

This invention provides a molecular identification and extraction method based on ReaxFF reaction kinetics simulation, belonging to the field of molecular identification and extraction technology. To obtain molecular structure information from reaction kinetics simulations, this invention obtains a dump source file containing bond orders and coordinates from the ReaxFF simulation output. It stores the bond order information at each time step in the bond source file, extracts and stores the atomic information of all molecules at the same time step, determines the target molecule features, identifies and stores the target molecule features based on the obtained atomic ID information of all molecules at the same time step, stores the coordinate information of the dump source file at each time step, and iterates through the atomic coordinate information and atomic IDs of all target molecules at the same time step to obtain the atomic coordinate information of the target molecule. This invention is applied to the identification and extraction of molecular structure information.

The application provides a new function of PIL13 for improving photosynthesis and plant height and a molecular identification reagent thereof. A transcription factor playing a pivotal role in transcription of photosynthesis genes is mined through transcriptome sequencing and construction of a gene regulation network. The PIL13 gene is obtained through screening and verification from the transcription factors, and the PIL13 gene can improve the leaf photosynthetic rate of gramineous plants, improve the light energy utilization rate, and improve the plant height. The method of construction, analysis and experimental verification of the gene regulation network of the application is reasonable, not only provides a new target for improvement of plant photosynthesis traits, but also can be used as a reference for research on gene regulation networks of other biological processes.

The application discloses a method for transgene and / or gene editing of Brassica crops mediated by co-transformation strategy, and belongs to the technical field of genetic engineering.The method comprises the following steps: transforming a development regulator vector and a target gene vector into Agrobacterium rhizogenes K599 respectively to prepare a mixed bacteria solution of double strains; infecting a Brassica vegetable explant; inducing adventitious buds after co-culturing; and screening different types of transgenic buds through fluorescence labeling, phenotype observation and molecular identification.The method is simple and efficient, and can effectively avoid adverse phenotypes such as transgenic plant deformity caused by continuous overexpression of the development regulator, and can obtain a gene editing homozygous mutant in the T0 generation, so that the method has a wide application prospect in the research of Brassica crop functional genomics, gene editing and crop genetic improvement.

This invention relates to the field of crop breeding technology, and in particular to a high-efficiency seed production method for greenhouse asparagus based on bumblebee pollination. Specifically, it includes: early and accurate identification of male and female plants using STS molecular markers during the seedling stage; scientifically matching male and female parents in a specific ratio and synchronously controlling their flowering periods; constructing a dedicated pollination net chamber with escape-proof buffer doors and microclimate fine-tuning design; implementing bumblebee-friendly green pest and disease prevention measures before flowering; and introducing bumblebees at a specific density for fully enclosed, high-efficiency pollination. This invention effectively overcomes the technical difficulties of high cost and low efficiency of traditional artificial pollination, as well as the problems of inconsistent flowering periods and heat stress on pollinating insects under conventional greenhouse conditions. Using the standardized breeding system of "molecular identification + spatial microenvironment + dedicated insect pollinator" constructed by this invention, the fresh fruit weight of a single plant can reach 300 grams, and the dry seed weight of a single plant can reach 56.2 grams. This not only eliminates interference from foreign pollen at the source, ensuring 100% purity, but also significantly increases the yield of hybrid seeds.

This invention discloses a method for systematically isolating and identifying Verticillium wilt of cotton, with the following steps: (1) disease sample collection and initial treatment; (2) strain culture: transfer the diseased tissue to a culture dish containing ampicillin PDA medium and culture it in a constant temperature incubator at 28°C for 3-5 days; (3) streak isolation and colony screening: place the culture dish under a microscope to directly observe the colony morphology. If the colony has the characteristic morphological features of Verticillium wilt of cotton, then the culture dish is confirmed to contain Verticillium wilt of cotton colonies; (4) single colony purification: from the culture dish containing Verticillium wilt of cotton colonies confirmed in step (3), pick a single colony with a sterile inoculation loop, transfer it to a new PDA medium for streak culture, and perform 2-3 consecutive passages for purification to obtain a purified strain; (5) DNA extraction and molecular identification; (6) pathogenicity verification inoculation; (7) re-isolation verification.

The present application relates to the field of plant genetic engineering technology, and particularly relates to the application of algal NySAT2 gene in regulating alfalfa crude protein and washing fiber. The present application uses gene synthesis and codon optimization technology to obtain the sequence of the coding region of the codon-optimized NySAT2 gene, constructs an algal NySAT2 gene overexpression vector, introduces the target gene into the alfalfa genome through the Agrobacterium rhizogenes-mediated genetic transformation method, and obtains the transgenic plant using the genetic transformation system. Through molecular identification and phenotype analysis, it is found that the alfalfa plants overexpressing the NySAT2 gene have a significantly increased crude protein content compared with the wild-type alfalfa plants, while the contents of acid detergent fiber and neutral detergent fiber are significantly reduced, effectively improving the forage quality and feeding value of alfalfa.

This invention belongs to the fields of biotechnology and precision oncology, and discloses a serum-free amplification method for circulating tumor cells (CTCs) based on apoptosis selectivity, along with a dedicated culture medium. This addresses the problems of existing CTC technologies, which rely on physical capture leading to impaired cell viability and low culture success rates. The invention involves mild density gradient centrifugation and erythrocyte lysis of anticoagulated whole blood from patients to obtain a mixed population of leukocytes containing CTCs. This population is then seeded into a serum-free, selective apoptosis medium for three-dimensional culture. The medium contains apoptosis inhibitors and specific growth factors. Utilizing the difference between tumor cells' anti-apoptosis and normal blood cells' susceptibility to apoptosis, leukocyte apoptosis is induced within 4-7 days, while simultaneously supporting CTC survival, adhesion, and clonal proliferation, enabling the amplification of individual CTCs into cell clusters. This method preserves the original activity and heterogeneity of CTCs, is simple to operate, and has good reproducibility, providing a highly active cell source for CTC molecular identification, drug sensitivity testing, and metastasis mechanism research.

The application discloses a light oil fraction rapid analysis method and system, comprising the following steps: performing chromatographic rapid analysis on multiple light fraction oil samples to obtain original spectrograms and label information; analyzing the multiple light fraction oil samples to establish a standard molecular library of light oil fractions; using the original spectrograms and the label information, constructing a data set, and establishing a gasoline type classifier model; taking a sample to be analyzed to perform chromatographic rapid analysis to obtain a new sample chromatogram, inputting the new sample chromatogram into the gasoline type classifier model to determine the type of the sample to be analyzed; finding similar samples close to the sample to be analyzed in the standard molecular library through a centroid method to obtain a matching molecular library and matching characteristic molecules of the type corresponding to the sample to be analyzed; constructing an optimization model to determine the positions of normal alkane peaks and characteristic molecule peaks in the sample to be analyzed; calculating the retention indexes of all molecules in the sample to be analyzed, identifying the molecules, and outputting a molecular identification result.