349 results about "Molecular identification" patented technology

The invention discloses a construction method of an agrobacterium tumefaciens mediated masson pine genetic transformation system, and belongs to the technical field of genetic engineering. According to the construction method of the agrobacterium tumefaciens-mediated masson pine genetic transformation system, immature masson pine cones are collected, embryogenic calluses are induced and cultured, the embryogenic calluses of the masson pine in a PEM III stage are used as a material, suspension pre-culture is performed to obtain an acceptor material, and the acceptor material is subjected to genetic transformation to obtain the agrobacterium tumefaciens-mediated masson pine genetic transformation system. The method comprises the following steps: firstly, carrying out tissue culture on a transgenic somatic embryo, infecting by adopting an agrobacterium infection solution with an OD600 value of 0.2-0.6, carrying out co-culture, recovery culture and screening culture, carrying out histochemical staining by adopting GUS, carrying out molecular identification to obtain a transgenic embryogenic callus, and carrying out maturation treatment to finally obtain the transgenic somatic embryo. The masson pine genetic transformation system established by the invention is stable in transformation performance and high in transformation positive rate, and when an acceptor material is masson pine embryonic callus with the genotype of 1-3-5, the transformation positive rate is as high as 100%.

The invention belongs to the technical field of molecular identification of aquatic organisms, and particularly relates to a high-sensitivity large yellow croaker germplasm identification primer combination as well as application and an identification method thereof. The primer composition is a first pair of primers LC-1, and the sequences of the first pair of primers LC-1 are as follows: an upstream primer LC-1F: 5 '-AATTCCACACGACTAATC-3'; the downstream primer LC-1R is 5 '-CTCAGCCGCCGTGCAATC-3', and the downstream primer LC-1R is 5 '- The sequences of the second pair of primers LC-2 are as follows: an upstream primer LC-2F: 5 '-AGGCATTAGGCACAAGG-3', a downstream primer LC-2F: 5 '-AGGCATTAGGCACAAGG-3', a downstream And the downstream primer LC-2R is 5 '-AAACCCCTAGCCTCGAAG-3'. The invention also discloses a kit for detecting the disease resistance of the traditional Chinese medicine. The two large yellow croaker germplasm identification primers provided by the invention have relatively high sensitivity and specificity, can be stably amplified in a DNA sample with extremely low concentration, and overcome the limitation that accurate identification cannot be realized due to low DNA concentration in a traditional method.

The invention belongs to the technical field of molecular identification of insects, and particularly relates to multiple PCR (Polymerase Chain Reaction) primers and a method for rapidly identifying thrips insect varieties. The invention discloses a multiple PCR primer and a method for rapidly identifying the variety of thrips, the identification primer and a multiple PCR system can rapidly, effectively and accurately identify the three types of thrips, and the sizes of PCR product strips of tobacco thrips, comb flower thrips and common large thrips are 104 bp, 237 bp and 439 bp respectively. The detection limit of the sensitive tobacco thrips is 500 copies, and the detection limits of the sensitive tobacco thrips and the sensitive common large thrips are both 100 copies. Compared with common PCR which can only identify one type of thrips at a time, the multiplex PCR system can complete identification of various types of thrips at a time, has the advantages of being easy to operate, low in cost, capable of saving time and the like, has very high value in the aspect of field thrips detection, and can be widely applied to the fields of plant protection and the like.

The invention relates to the technical field of plant genetic engineering, and particularly provides a rice endosperm tissue specific promoter pPROLM26 and application thereof. According to the invention, a tissue-specific promoter with a nucleotide sequence as shown in SEQ ID NO.1 is separated from a rice alcohol-soluble protein gene, the tissue-specific promoter is cut off for four times, and then the cut-off promoter fragment with an endosperm-specific promoter function as shown in SEQ ID NO.2-5 is obtained after molecular identification is carried out on the cut-off promoter fragment and an RUBY visual marker; the betaine content of the expression product can reach 2.5% of the dry weight of the seeds through determination, and the method has very high application value in the field of plant synthetic biology. The invention further discloses application of the promoter or the truncated promoter fragment in the aspects of improving crop seed quality, improving crop traits, cultivating new varieties of transgenic plants by using key elements of the promoter or expressing high value-added proteins by using a seed bioreactor and the like.

The invention discloses a DNA bar code-based honeysuckle stem and red-white honeysuckle stem molecular identification method, and belongs to the technical field of traditional Chinese medicine detection. DNA bar code genes for identifying honeysuckle stems and red and white honeysuckle stems are cell nucleus genes Lj7A621T37 with INDEL difference and / or chloroplast genes trnH-psbA with SNP (Single Nucleotide Polymorphism) difference; a PCR (Polymerase Chain Reaction) amplification upstream primer and a PCR amplification downstream primer of the nuclear gene Lj7A621T37 with INDEL difference are respectively as shown in SEQ ID NO. 1 and EQ ID NO. 2; a PCR (Polymerase Chain Reaction) amplification upstream primer and a PCR amplification downstream primer of the chloroplast gene trnH-psbA with SNP (Single Nucleotide Polymorphism) difference are respectively as shown in SEQ ID NO. 3 and SEQ ID NO. 4. The accurate identification result can be obtained by utilizing the two pairs of specific primers and the two specific sites, and the detection method is efficient and convenient and can be used for identifying the authenticity of the honeysuckle stem medicinal material.

The invention provides a local livestock and poultry variety identification method based on an SNP marker, and relates to the field of variety identification, and the method comprises the following steps: S1, genotype data acquisition, S2, genotype data quality control, S3, variety identification standard library establishment, and S4, bloodline content evaluation. According to the method, on the basis of a standardized molecular identification analysis process of genomic SNP marker bloodline content evaluation, genotype information of a core SNP site is analyzed, so that whole analysis process processing from original genotyping data to variety identity judgment is realized; the method can provide standardized, convenient and efficient molecular detection technical support for protection and utilization of genetic resources of local livestock and poultry in China, germplasm innovation and industrial development.

A protein nanopore comprising one or more sensing module and a method for characterizing a target molecule using the protein nanopore.

The present invention relates to the field of plant genetic engineering technology, specifically providing a rice endosperm tissue-specific promoter, pPROLM26, and its applications. The present invention isolates a tissue-specific promoter with the nucleotide sequence shown in SEQ ID NO. 1 from a rice alcohol-soluble protein gene. This tissue-specific promoter is truncated four times and then molecularly identified with a RUBY visualization marker to obtain truncated promoter fragments shown in SEQ ID NOs. 2 to 5, each with endosperm-specific promoter function. The expression product has been measured to contain up to 2.5% betaine of the seed dry weight, demonstrating its high application value in the field of plant synthetic biology. The present invention further discloses applications of the promoter or truncated promoter fragments in improving crop seed quality, improving crop traits, cultivating new transgenic plant varieties using key elements of the promoter, or expressing high-value-added proteins using seed bioreactors.

The invention relates to a gene ZmILR1 for controlling the size of a corn kernel as well as a molecular identification method and application of the gene ZmILR1, and belongs to the technical field of plant genetic breeding and functional gene research. The ZmILR1 gene encodes an IAA-amino acid hydrolase, mutation of the IAA-amino acid hydrolase causes corn kernels to become smaller, and overexpression of the gene can create a corn material with increased kernel length, kernel width and hundred-kernel weight. A functional molecular marker A1A4 is developed according to the sequence difference of the mutant gene and a wild type gene at the DNA level, and can be used for rapid identification and screening of small-grain corn materials. The invention provides a theoretical basis and an application tool for analyzing a molecular mechanism of the corn grain size and developing molecular marker-assisted breeding and hybrid seed breeding, and provides important gene resources and technical support for high-yield and high-quality molecular breeding of corn.

The invention relates to the field of growth-promoting bacteria, in particular to screening and application of saline-alkali soil crop growth-promoting bacteria, and discloses a screening method and application of the saline-alkali soil crop growth-promoting bacteria. Through gradient domestication, multi-index function verification and molecular identification, a strain which is salt-tolerant, can efficiently solubilize phosphorus (more than 150 mu g / mL), can secrete IAA (more than 30 mg / L) and has nitrogenase activity more than 20 IU / L is screened from rhizosphere soil with salt concentration of 0.8%-1.2% and pH of 7.8-8.5. Screened strains are compounded with a carrier (the ratio of decomposed sheep manure to attapulgite to humic acid to xanthine is (70-75): (20-25): (8-10): (0.3-1)), the complex microbial inoculant is prepared through secondary fermentation, and a final field test shows that the yield of corn can be increased by 18%-32%, the pH of soil is reduced by 0.5-1.0, the salinity is reduced by 0.2%-0.5%, the saline-alkaline environment is remarkably improved, and the complex microbial inoculant has a wide application prospect.

The invention provides an SNP (Single Nucleotide Polymorphism) molecular marker primer combination for molecular identification of soft fiber areca seed resources and application of the SNP molecular marker primer combination, and belongs to the technical field of SNP molecular markers. Genetic background correlation analysis of fruit fiber phenotypic characteristics is carried out based on constructed areca seed core germplasm resources, SNP molecular marker primers are developed according to areca seed fiber specific sites, and the SNP molecular marker primer development comprises four pairs of specific primers; the invention also provides a method for detecting the germplasm resource of the areca catechu by using the SNP molecular marker primer. The method can be used for rapidly and accurately detecting the genotype of the germplasm resource of the areca catechu at low cost and high throughput. The four pairs of specific primers can be used for identifying the germplasm resources of the soft-fiber areca catechu, and have important application significance on scientifically protecting the diversity of the germplasm resources of the areca catechu, researching genetic protection and genetic evolution of the areca catechu resources and genetic breeding of the areca catechu.

The invention relates to the technical field of molecular detection, in particular to a specific primer combination for distinguishing enteromorpha from sibling species, application of the specific primer combination and a molecular identification method. The primer combination comprises an upstream primer SEQ1 and a downstream primer SEQ2, the primer sequence of the SEQ1 is as shown in SEQ ID NO.01, and the primer sequence of the SEQ2 is as shown in SEQ ID NO.02. The primer combination is designed based on the difference of genomes of two algae, has high specificity and sensitivity, can effectively avoid cross reaction, and ensures the accuracy of a detection result. Meanwhile, in combination with a real-time fluorescent quantitative PCR (qPCR) technology, the method can effectively quantify the abundance of enteromorpha in an environmental sample or a laboratory sample, and provides reliable technical support for early warning, traceability analysis and prevention and control strategy formulation of green tide outbreak.

The invention belongs to the technical field of plant molecular biology, and discloses a PCR amplification primer suitable for molecular identification of amorphophallus konjac and amorphophallus albus hybrids, the PCR amplification primer comprises a ZJ20 primer, and the sequence of the ZJ20 primer is F: CAGACCATAATCAGCATG; r is GTCTCCACT GCCGTACCTT, and R is GTCTCCACT The molecular identification method comprises the following steps: step 1, extracting genome DNA of a to-be-detected konjak sample, carrying out amplification by using the PCR primer, then carrying out first-generation DNA sequencing, and if a hybrid genotype A / T is observed at three positions of 170-180bp, 190bp-200bp and 210bp-220bp, determining that a hybrid first filial generation of amorphophallus konjac and amorphophallus albus is obtained, and a reaction system for PCR amplification in the step 2 is as follows: the reaction total volume is 25 microliters, and the reaction time is 30 minutes; a reagent contained in the step 3 is as follows: 12.5 mu L of a 2 * PCR Mix premixed solution, 0.3 mu M of R and F primers and 30 ng of a sample genome DNA solution, finally, sterile ultrapure water is used for complementing the deficient volume, and the amplification process of the PCR reaction in the step 3 is as follows: pre-denaturation at 95 DEG C is performed for 5 minutes, then denaturation at 95 DEG C is performed for 45 seconds, annealing at 56 DEG C is performed for 45 seconds, extension at 72 DEG C is performed for 45 seconds, and 35 amplification cycles are performed in total.

The invention relates to a method for extracting genome DNA of plants rich in secondary metabolites and a buffer solution, and belongs to the technical field of molecular biology and botany. The method solves the technical problems of low DNA extraction efficiency, poor purity, easy degradation and the like when a traditional DNA extraction method is used for treating plant tissues rich in secondary metabolites such as alkaloid, polyphenol and the like. Comprising the following steps: adding a complexing agent such as polyethylene glycol or polyvinylpyrrolidone when grinding plant tissues in a liquid nitrogen environment; splitting by using a cell wall splitting buffer solution containing dithiothreitol and a nonionic surfactant; carrying out DNA release and extraction at 60-70 DEG C by adopting a CTAB (Cetyltrimethyl Ammonium Bromide) extraction buffer solution containing polyethylene glycol and papain; and then purifying and precipitating to obtain high-purity genome DNA (Deoxyribose Nucleic Acid). The method can effectively remove alkaloid, protein and other impurities, significantly improves the DNA yield and purity, and is suitable for genome sequencing, genetic resource protection, medicinal plant molecular identification and the like of plants with high secondary metabolites such as Stephania kwangsiensis and the like.

The invention relates to a molecular identification method of insects, in particular to species-specific primers and a method for identifying chrysopa septempunctata. According to the method for determining the difference sites of the species based on comparison of representative haplotype sequences of the species, the sequence composition difference, caused by geographical isolation, of COI genes in the species is considered, primers capable of distinguishing different species are designed, particularly, combination of similar sequences of the primers and non-target species is reduced between similar species, and the specificity of the primers is improved. Therefore, the possibility of non-specific amplification is reduced. The species-specific primer for identifying chrysopa septempunctata, provided by the invention, is high in sensitivity, and the lowest requirement on the concentration of DNA to be detected is 0.0655 ng / mu L which is lower than the lowest detection concentration 0.143 ng / mu L of the disclosed species-specific primer for chrysopa formosa brauer.

The invention provides a method for enriching and separating Blauria bacteria in human intestinal tracts under common laboratory conditions. An anaerobic culture medium and a special enrichment culture are prepared, and a unique culture device and an operation process are combined, so that the problems of sample complexity, harsh growth conditions, lack of a selective culture medium and the like are solved. The method comprises the steps of culture medium preparation, enrichment culture, nucleic acid extraction, high-throughput sequencing, strain separation, molecular identification and the like, has the advantages of low cost, simplicity and convenience in operation, high-efficiency screening and the like, and provides technical support for research and application of the Blauria bacteria.

The invention belongs to the technical field of medicines, and discloses a near-infrared fluorescent probe taking an alpha, beta-unsaturated acryloyl ester unit as a response site of sulfydryl. The fluorescent probe has great advantages in the aspects of biological small molecule identification, protein marking and disease diagnosis, and is one of the most convenient and most popular tools for exploring a complex biological process. The fluorescent probe molecule capable of releasing red fluorescence after being activated by sulfydryl is obtained for the first time by connecting a fluorophore with an alpha, beta-unsaturated acryloyl ester unit. The fluorescent probe molecule disclosed by the invention can be used for specifically detecting sulfydryl in an organism, so that the aim of accurately monitoring the sulfydryl in a cell is fulfilled.

The invention relates to the technical field of plant genetic engineering, in particular to application of an algae NySAT2 gene in regulation and control of alfalfa crude protein and detergent fiber. According to the invention, a sequence after codon optimization of an NySAT2 gene coding region is obtained by using gene synthesis and codon optimization technologies, an algae NySAT2 gene overexpression vector is constructed, a target gene is introduced into an alfalfa genome by using an agrobacterium rhizogenes mediated genetic transformation method, and a transgenic plant is obtained by using a genetic transformation system. Molecular identification and phenotypic analysis show that on the premise of keeping normal growth vigor (plant height and biomass have no significant difference from those of a wild type), the crude protein content of a medicago sativa plant with the overexpressed NySAT2 gene is remarkably increased compared with that of the wild type medicago sativa plant, and meanwhile, the contents of acid detergent fibers and neutral detergent fibers are remarkably reduced, so that the content of the crude protein in the medicago sativa plant with the overexpressed NySAT2 gene is remarkably increased. The forage quality and the feeding value of the medicago sativa are effectively improved.

The application discloses a high-sweetness-amino-acid-content Flammulina velutipes variety and a MNP molecular identification method and application thereof. The Flammulina velutipes variety has excellent properties, the cap is not easy to open, the content of sweet amino acids is high, the diversified market demand is met, the factory annual bottle cultivation is suitable, and the Flammulina velutipes variety has a good application and popularization prospect. The MNP molecular fingerprint of the Flammulina velutipes variety 'Shangyan A111' is comprehensive multiple amplification and sequencing technology, sequence analysis of all marker sites of multiple samples can be performed at one time, compared with ISSR, RAPD, SSR and other molecular markers and mushrooming test, the MNP molecular fingerprint has the advantages of high throughput, multi-target, high sensitivity and high precision. The MNP molecular fingerprint of the Flammulina velutipes variety 'Shangyan A111' has specificity and specificity in identifying the Flammulina velutipes strain 'Shangyan A111'.

This invention relates to a rapid identification method Lactobacillus plantarum The method for identifying strains, belonging to the field of species and strain identification, is based on the differences in three DNA barcode sequences. Lactobacillus plantarum CGMCC NO: 26171 strain from Lactobacillusplantarum This method allows for rapid identification from other strains of the same species. Compared to traditional morphological identification methods, the standard gene sequence obtained is beneficial for the molecular identification of *Lactobacillus plantarum* strains, effectively shortening the identification time. Three pairs of DNA barcoding primers enable specific amplification of the test strain, allowing for rapid identification. Lactobacillus plantarum strains.

To provide a gene testing method and a gene testing kit for analyzing a variable region of a TCR or a BCR in single-cell analysis.SOLUTION: A method including a step of capturing mRNA eluted from a single cell using an RT probe immobilized on a solid-phase surface and including a common sequence, a cell identification barcode, a first molecular identification barcode, and an oligo(dT) sequence, a step of performing a reverse transcription reaction in a reverse transcription reaction solution containing a TSO using the mRNA as a template to generate cDNA having a sequence added to a 3' end, a step of degrading the mRNA, a step in which a primer for inserting a second molecular identification barcode binds to a constant region adjacent to a variable region of a TCR or a BCR and includes the second molecular identification barcode, a step of ligating cDNA extended from the primer with a ligase, a step of amplifying a fragment including the second molecular identification barcode and the variable region of the TCR or the BCR using a primer, a step of performing sequence analysis, and a step of associating sequences.SELECTED DRAWING: Figure 2

The invention relates to the technical field of crop molecular identification, in particular to a crop variety identification method based on a multi-allelic variation SNP (Single Nucleotide Polymorphism) marker. The variety identification method comprises the following steps: carrying out genotype detection on a to-be-detected crop based on a multi-allelic variation SNP marker set, comparing a detection result with a contrast variety genotype or fingerprint database, and judging the to-be-detected crop according to a comparison result, the multi-allelic variation SNP marker set is obtained by mining whole genome sequencing data of the representative material of the to-be-detected crop; the fingerprint database is obtained by performing hybridization sequencing on the crops to be detected based on the multi-allelic variation SNP marker set. The detection method provided by the invention can be used for authenticity identification, substantive derived variety identification, genetic relationship identification, germplasm resource genetic evaluation and breeding of crops, and provides a new thought and method for crop variety identification, variety right protection, germplasm resource evaluation and the like.

The present application relates to the technical field of molecular detection, and particularly relates to a specific primer combination for distinguishing Enteromorpha prolifera and related species, application and molecular identification method thereof. The primer combination comprises an upstream primer SEQ1 and a downstream primer SEQ2. The primer sequence of SEQ1 is shown as SEQ ID NO. 01, and the primer sequence of SEQ2 is shown as SEQ ID NO. 02. The primer combination of the present application is designed based on the genomic difference of two kinds of algae, has high specificity and sensitivity, can effectively avoid cross reaction, and ensures the accuracy of the detection result. Meanwhile, combined with the real-time fluorescent quantitative PCR (qPCR) technology, the method can effectively quantify the abundance of Enteromorpha prolifera in environmental samples or laboratory samples, and provides reliable technical support for early warning, tracing analysis and prevention and control strategy of green tide outbreak.

The invention discloses a bivalve mollusc identification method combining molecular identification with three-dimensional vision. Firstly, a bivalve mollusc sample is dissected and divided into adductor muscle tissue and shell, DNA of the adductor muscle tissue is extracted, COI gene sequence is amplified and sequenced by PCR, and molecular tags of the bivalve mollusc sample are obtained; then, the shell of the shell is restored, photos of the shell of the shell at different angles are taken by a three-dimensional model shooting device, and the photos are processed to obtain a three-dimensional model of the bivalve mollusc sample; then, a model for classification and identification is obtained based on three-dimensional model data training, and finally, the three-dimensional model data of the shell sample to be tested is input into the model. The model can realize classification and identification of unknown shells containing only three-dimensional model data, and the classification accuracy rate is above 80%, which solves the relevant difficulties of identification of morphologically similar shells and non-invasive identification, and has practical application significance for improving the breeding and production efficiency of bivalve molluscs, as well as the identification of cryptic species in ecological detection.

The invention relates to the technical field of molecular identification of crops, in particular to a corn triallelic variation SNP site combination and application thereof. The triple allelic variation SNP site combination comprises 40 triple allelic variation SNP sites, and the triple allelic variation SNP site combination comprises nucleotide sequences as shown in SEQ ID NO.1-40. The SNP site combination is obtained through screening and can be applied to corn variety authenticity identification, genetic relationship identification, purity identification and plant breeding. Compared with diallelic variation sites, the 40 triallelic variation SNP sites provided by the invention have the advantages that the variety recognition capability of a single site is greatly improved, and the development and application of the 40 triallelic variation SNP sites expand the range of available marker sites of corn on the genome level; the invention provides a new thought and method for corn variety identification, genetic relationship evaluation and the like, and has important value in the field of corn breeding.

The invention belongs to the field of biological quarantine, and particularly relates to a method for separating and identifying pigeon-derived liver escherichia coli. Escherichia coli separation and identification are carried out on disease samples from Guangdong, Guangxi, Guizhou, Hainan and the like, and the epidemicity of the disease samples is determined; collecting livers of acute dead young meat pigeons, and separating strains; carrying out gram staining, physiological and biochemical identification and molecular identification on the separated strain; the growth and reproduction cycle and hemolysis characteristics of the strain are detected, 76 Escherichia coli infection occurs in 113 disease samples, and the infection rate is 67%. According to the method, laboratory diagnosis of infectious diseases of the meat pigeons is systematically completed through sample collection and separation, culture and identification of strains, it is determined that the young meat pigeons are infected by escherichia coli, and a foundation is laid for subsequent antibiotic-free green breeding of the meat pigeons resisting escherichia coli infection.

This invention provides a combination of SNP molecular marker primers for the molecular identification of soft-fiber areca nut germplasm resources and their applications, belonging to the field of SNP molecular marker technology. Based on the constructed core areca nut germplasm resources, this invention conducts genetic background association analysis on the phenotypic characteristics of fruit fibers and develops SNP molecular marker primers based on areca nut fiber-specific loci. The development of these SNP molecular marker primers includes four pairs of specific primers. This invention also provides a method for detecting soft-fiber areca nut germplasm resources using SNP molecular marker primers. This method can rapidly, accurately, cost-effectively, and with high throughput detect the genotype of soft-fiber areca nut germplasm resources. The four pairs of specific primers of this invention can be used to identify soft-fiber areca nut germplasm resources, which has important application significance for the scientific protection of areca nut germplasm resource diversity, research on the genetic conservation and evolution of areca nut resources, and areca nut genetic breeding.

The application discloses a kind of molecular identification methods of sex-linked luhua feather homozygote of luhua chicken, belong to molecular marker assisted breeding technical field.The application can quickly and accurately detect homozygous sex-linked luhua chicken individual by designing KASP detection primer for detecting three sex-linked luhua feather color strong linkage sites, and the genotype of three sites can quickly and accurately detect homozygous sex-linked luhua chicken individual, when SNP2 (C->A) at 389bp of the first intron of CDKN2A gene, SNP3 and SNP4 at 172bp (T->A) and 174bp (T->C) of the first exon are AA, AA and CC respectively, then the chicken to be measured is homozygous sex-linked luhua chicken, otherwise the chicken to be measured is heterozygous sex-linked luhua chicken or non-sex-linked luhua chicken.The application provides an effective technical means for purifying sex-linked luhua feather color gene of luhua chicken, and helps to speed up the breeding speed of sex-linked luhua chicken strain homozygote and its matching system.

The invention discloses a rapid molecular identification method for codonopsis pilosula, pilose asiabell root and tangshen, which comprises the following steps: designing a specific primer of codonopsis pilosula, and amplifying a bar code sequence fragment of an intron region of chloroplast DNArpl16 of a codonopsis pilosula sample; according to the basic groups of the 634th site and the 741th site from the 5'end of the bar code sequence of the intron region of the amplified DNArpl16, identifying the radix codonopsis when the 634th site is T and the 741th site is T, determining that the detected medicinal material is codonopsis tangshen; when the 634th site is A and the 741st site is G, the detected medicinal material is Codonopsis pilosula; when the 634th site is T and the 741st site is G, the detected medicinal material is codonopsis pilosula or pilose asiabell root, that is, the pilose asiabell root in the sequence fragment has two genotypes. The identification method provided by the invention can be used for rapidly and accurately identifying original plants, medicinal materials, decoction pieces, seeds, seedlings and the like of codonopsis pilosula, and is stable and high in specificity.

The invention relates to the technical field of germplasm resource identification, in particular to a molecular marker, a primer and a kit for identifying golden-screen geese and Taihu Lake geese and application of the molecular marker, the primer and the kit. According to the present invention, the difference between the pantoyl mercaptoethylamine enzyme (VNN1) gene sequences of the local Guizhou golden geese and the Jiangsu Taihu goose is utilized to find that the five polymorphic sites capable of accurately identifying the local Guizhou golden geese and the Jiangsu Taihu goose can be accurately identified, and the primers are designed based on the polymorphic sites so as to identify the local Guizhou golden geese and the Jiangsu Taihu goose; two different varieties of meat geese, namely local white geese in Guizhou and white geese in Taihu Lake, can be quickly and accurately identified, so that a molecular identification means is provided for purifying resources of the local variety of meat geese and accurately screening specific functional genes in germplasm resources of the local meat geese in a pure breeding and breeding process of the local variety of meat geese; moreover, a scientific basis is provided for retention of dominant genes of the local meat goose variety, and considerable economic benefits and use values are achieved.