894 results about "Molecular identification" patented technology

The invention relates to the field of specific molecular identification and diagnosis reagent and in particular discloses a near infrared fluorescent dye with structural formulas I and II; and the invention also discloses a near infrared molecular probe which is obtained through covalent bonding between the near infrared fluorescent dye with the structural formulas I and II and a ligand of specific molecules. The near infrared molecular probe can be used for early diagnosis of turmour diseases.

Devices for controlling the capture, trapping, and transport of macromolecules include at least one fluidic transport nanochannel that intersects and is in fluid communication with at least one transverse nanochannel with (shallow) regions and/or with integrated transverse electrodes that enable fine control of molecule transport dynamics and facilitates analyses of interest, e.g., molecular identification, length determination, localized (probe) mapping and the like.

The invention discloses a core SNP sites combination maizeSNP384 for building of a maize DNA fingerprint database and molecular identification of varieties, and an application of the core SNP sites combination. The invention provides applications of 384 SNP sites in any one of the following conditions: (1) building of the maize DNA fingerprint database; (2) detecting of the authenticity of maize varieties; (3) genetic analysis of corn germplasm resources; and (4) molecular breeding of maize, wherein the physical positions of the 384 SNP sites are determined by comparison on the basis of a whole genome sequence of the maize variety B73; the version number of the whole genome sequence of the maize variety B73 is B73 RefGen V1; and the 384 SNP sites are MG001-MG384. An experiment proves that the 384 SNP sites can be applied to building of the maize variety DNA fingerprint database, identification of the variety authenticity, dividing of germplasm resource groups, and other related researches.

The invention relates to the field of biotechnology and discloses a molecular identification method for Isaria cicadae (CGMCCNO.3453). The method includes the steps of extracting genomic DNA of the strain, performing amplification of the DNA in the transcription spacers of the genome using primers ITS5 and ITS4, and recovering and purifying the obtained DNA fragments; performing bidirectional sequencing of the purified DNA fragments; aligning the obtained DNA sequences with SEQIDNO:3; and identifying the strain as Isaria cicadae (CGMCCNO.3453) if the ITS sequence of the strain has the same content and order of arrangement as SEQIDNO:3. The inventive method can rapidly, reliably and accurately identify Isaria cicadae (CGMCCNO.3453), and the identification method is not influenced by environmental factors.

The invention discloses a tumor neoantigen detection method and device based on next-generation sequencing, and a storage medium. The tumor neoantigen detection method disclosed by the invention comprises the following steps of variation detection, MHC (Major Histocompatibility Complex) molecular identification, variation annotation, variation peptide fragment prediction, variation peptide fragment MHC/II type affinity prediction, antigen expression abundance detection, clonality analysis and candidate tumor neoantigen comprehensive scoring sorting, wherein the candidate tumor neoantigen comprehensive scoring sorting comprises the step of scoring sorting on the neoantigen according to MHC affinity, antigen expression abundance and clonality according to a formula I. According to the tumorneoantigen detection method, a next-generation sequencing comparison file is used as a basis to carry out mutation and MHC detection, in addition, the candidate tumor neoantigen is scored from three dimensions including MHC I/II type affinity, antigen expression abundance and clonality so as to the screen high-quality tumor neoantigen, and a foundation is laid for immunotherapy on the basis of thetumor neoantigen.

The invention discloses a molecular identification method of an atlantic salmon. The molecular identification method comprises the following steps of 1, extraction of fish DNA (deoxyribonucleic acid); 2, PCR (polymerase chain reaction) augmentation; 3, enzyme digestion and electrophoresis detection; and 4, identification. The atlantic salmon can be accurately determined in four steps. The molecular identification method of the atlantic salmon has the characteristics of being simple to operate, rapid and accurate, low in price, low in requirement on an instrument and equipment, and the like.

The invention discloses a DNA (deoxyribonucleic acid) barcoding based molecular identification method and CO I sequence of a leech (whitmania pigra). Polymerase chain reaction (PCR) is carried out by using the method to amplify a CO I gene of the leech, a PCR product is sent to a sequencing company to be sequenced after being verified by agarose gel, the sequencing results are subjected to manual proofreading and sequence splicing and are compared with public sequences, and the tissue to be tested can be judged to be the source of whitmania pigra if the homology of the sequencing results and a gene sequence SEQ ID NO.1 is above 99%. The whitmania pigra variety is quickly and accurately identified by adopting the reliable DNA molecular identification technology, thus improving the accuracy and the reliability and ensuring the safety of the leech as a medicine.

The invention discloses red yeast rice rich in Monacolin K and a preparation method of the red yeast rice. The preparation method comprises the steps of taking 40 monascus strains which are obtained in a separated mode as objects, and screening out a monascus strain, namely FG-8, wherein the monascus strain produces the Monacolin K in a high-yield mode and produces no citrinin, and the Monacolin K content of a fermentation product of the monascus strain is 2.46 mg/g and 2.29 times that of a type strain ACCC 30501. Through molecular identification, the strain is determined as red monascus. The red monascus, namely the FG-8, is treated as an original strain, 26 different varieties of rice raw materials are screened, when the FG-8 is founded, polished rice of a rice variety, namely 'California red rice', is treated as substrates for conducting fermentation, and the red yeast rice rich in the Monacolin K is obtained. The red yeast rice is simple in preparation method, the Monacolin K content measured under the optimum fermentation condition is 4.64 mg/g and approximately 8.30 times that of Furuta red yeast rice on the market, and no citrinin is detected.

Some embodiments of the present disclosure are directed to a compound 5(6)-mercapto-1H-benzo[d]imidazole-2-carboxamide (“BIA”) which yields enhanced signals for recognition tunneling. Other embodiments are directed toward methods for producing such compounds as well as apparatuses and systems which utilize such compounds for recognition tunneling for molecule identification/sequencing (for example).

The invention relates to a method for extracting high-molecular-weight genome from animal feces. The method comprises the steps of sample treatment, bacterial cell lysis and extraction of DNA (Deoxyribonucleic Acid). Specifically, the method comprises the steps of: dissolving an animal feces sample with aseptic PBS (Phosphate Buffered Saline) buffer, carrying out low-speed centrifuging and filtering to remove food residues in the feces, collecting bacterial cells, performing wall breaking on the bacteria by adopting lysozyme, decolorizing peptidase, protease and SDS (Sodium Dodecyl Sulfate), extracting with a mixture of phenol, chloroform and isoamyl alcohol as well as a mixture of chloroform and isoamyl alcohol, precipitating with isopropanol and sodium acetate, washing and drying, dissolving, and digesting RNA (Ribonucleic Acid) with RNAse (Ribonuclease). According to the invention, the method is simple in operation; and the DNA molecular weight of the extracted genome achieves more than 40kb, so that the researches on microorganism molecule ecological diversity, molecular identification, target gene amplification, macro genome library construction and the like are met.

The invention provides a preparation method of glyphosate resistant transgenic rice. The method comprises the following steps: converting an expression vector containing glyphosate resistant gene into Agrobacterium tumefaciens, invading rice calluses by the Agrobacterium tumefaciens, transferring the calluses to a selective aluminum containing glyphosate for screening, choosing resistant calluses, differentiating, rooting, hardening-seedling, and transplanting to obtain glyphosate resistant transgenic rice. A molecular identification result shows that an exogenous gene is stably expressed. The obtained glyphosate resistant transgenic rice contains glyphosate herbicide resistant gene, contains no other screening marker genes, and can be used as a commercial herbicide resistant rice kind to reduce the reduce the later process and accelerate the breeding pace. The method has the advantages of simple operation, low copy number of transgenic plants, stable heredity properties, and good market application prospect.

The invention pertains to the field of rape breeding and discloses a method for seed selection and identification of a novel dwarf compact type rape adapting to high-density planting and mechanized harvesting. The rape of Variety 5148-2 with apreservation number of CCTCC-P200601 that is bred by Huazhong Agricultural University and yellow-seed rape of DH series YN90-1016 that is synthesized artificially in Canada are taken as seed-parents and crossbred to obtain F1. In inflorescence, the main anthotaxy of an F1 plant and the flower buds with a size ranging from 2.8mm to 3.8mm at the upper branches of the P1 plant are selected, and then chromosome doubling with colchicine and microspore culture are carried out successively to obtain the segregation populations of double haploid. Filed phenotypic characteristic identification are applied for years in various spots to the segregation populations; variant rape materials with five major traits, namely, stem length, branch trait, length of anthotaxy, quantity of silique and growth period differing from those of the common rape and dwarf, compact plant, smallish and acervate branches and main anthotaxy, concentrative florescence and compact pod bearing layers phenotypes are selected to obtain the novel dwarf compact type rape adapting to mechanized harvesting. The invention also discloses a method for molecular identification.

The invention discloses a method for identifying a DNA bar code molecule of an earthworm and a COI sequence of the DNA bar code molecule of the earthworm. The method comprises the steps of amplifying the gene of a sample COI through polymerase chain reaction (PCR), verifying the PCR product by agarose gel, then sending to a biological sequencing company for sequencing, according to the sequencing result, sequence splicing through manual correcting, comparing with a public sequence, then confirming the tissue to be tested as the source of pheretima aspergillum or pheretima vulgaris if the homology of the spliced sequence and the gene sequence SEQ ID NO.1-NO.4 is over 99%. By adopting the reliable DNA molecular identification technology, the method can rapidly and accurately identify the earthworm varieties specified by the 'pharmacopoeia of China' and non-medicinal material earthworm varieties without conducting morphological identification on the earthworm varieties, can further distinguish the pheretima aspergillum varieties from pheretima vulgaris varieties, improves the accuracy and reliability, and guarantees the safety of the earthworm as the medicinal material.

The invention belongs to the technical field of food, in particular relates to the field of pickled vegetables in food, and particularly relates to fresh keeping liquid for effectively preventing sack expands of a pickled pepper product and a preparation method of the fresh keeping liquid. Microorganisms causing the gas production of the pickled pepper product are provided by various means for discriminating gas production germs such as traditional separation purification, molecular identification, duchenne small tube and the like to be pseudomonas, saccharomyces cerevisiae and kazachstania turicensis, an appropriate bacteriostatic agent can be selected on purpose, and the fresh keeping liquid for effectively preventing the sack expands of the pickled pepper products. Compared with the similar products (general pickled vegetable preservatives in the market), the fresh keeping liquid is more purposeful and better in effect, reduces the consumption of the preservatives and avoids the abuse risk of the preservatives; and moreover, the production method is simple, and the cost is low.

The invention discloses an ISSR-SCAR (inter simple sequence repeat-sequence characterized amplified region) marker capable of identifying Wujiang brassica chinensis and an identification method of the marker. The method includes: performing total DNA (deoxyribose nucleic acid) extraction, PCR (polymerase chain reaction) amplification, primer selection and ISSR-SCAR detection on Wujiang brassica chinensis and other 10 brassica chinensis varieties, and finally determining an ISSR-SCAR molecular marker and a molecular identification method of the Wujiang brassica chinensis. A primer shown in the sequence such as SEQ ID NO:1 is used for amplification to obtain a specific fragment shown in the sequence such as SEQ ID NO:2. According to the sequence, a pair of primers in the sequences such as SEQ ID NO:3 and SEQ ID NO:4 is designed. The marker is simple to operate, stable and reliable, is the first molecular identification marker applied to the variety of Wujiang brassica chinensis, can be used for quickly identifying the variety and has significance of protecting germplasm resources of geographical indication products.

The invention provides a rice CYP704B2 gene mutant and applications thereof, belonging to the technical field of genetic engineering. A nonglutinous rice variety 93-11 is subjected to induced mutation by cobalt 60 radiation, so as to cause deletion of two G basic groups behind 1267 basic groups of a rice CYP704B2 gene; the rice CYP704B2 gene mutant is named as cyp704B2-2, and a nucleotide sequence thereof is shown as SEQ ID No.1 and further verifies that the mutant causes rice recessive genic male sterility; the rice CYP704B2 gene mutant can be used for preparing transgenic rice with recessive genic male sterility, and plays an important role in genetic improvement and breeding of rice germplasm resources. The invention also provides a molecular marker identification method of the mutant and applications of the method in breeding and seed production.

The invention belongs to the technical filed of biology, and relates to a molecular identification method of a cytoplasm male sterile restoring line, and concretely provides a CAPS-labeled primer used for identifying the cytoplasm male sterile restoring line, and a nucleotide sequence is shown as SEQ ID NO. 2 and SEQ ID NO.3. The method employs the CAPS label for a polymorphism detection of molecule level of a cytoplasm male sterile restoring line material, The method has the advantages of fast identification speed, high accuracy and simple operation, and is very suitable for indoor identification of purity of the cytoplasm male sterile restoring line material and molecular marker-assisted selection of a new restoring line material.

The invention belongs to the technical field of detection of related products of animal origin components including meat and source thereof, and particularly provides a pair of primers capable of detecting the origin components of eight kinds of animals of goat, sheep, buffalo, cattle, pig, deer, camel and yak at the same time in a PCR reaction, the molecular identification technology in the operation process, a kit comprising the primers, and an application method. The nucleotide sequences of the primers are shown in the SEQ ID 1 and the SEQ ID 3. The PCR method is based on the PCR technology, has the advantages of being simple in operation, strong in specificity, low in equipment requirement, and the like, importantly, can detect the origin components of the eight kinds of animals at the same time in once PCR, can be used for adulteration detection of the food meat and the meat products and the tracking source identification of the animal origin components in feed, is simple and quick, and improves the detection efficiency.

The invention belongs to the technical field of meat product molecular detection and particularly relates to a molecular identification method and kit of a meat product made of beef as well as application of the molecular identification kit. The molecular identification method comprises the following steps: (1) primer designing: designing a specific primer according to the sequence of the TNFRSF10A gene of a bos taurus, wherein the sequence of the specific primer is shown in SEQ ID NO: 2 and SEQ ID NO: 3. (2) creating a PCR amplified reaction method, that is, amplifying the genome DNA of the bos taurus through the specific primer so as to obtain amplified specific fragments; (3) analyzing the amplified PCR products, that is, analyzing the amplified PCR products through agarose gel electrophoresis; according to gel-imaging, observing whether target fragments appear or not, and judging whether the target fragments contain the components of the genome DNA of the bos taurus or not. The molecular identification method has the characteristics of simplicity, convenience, quickness, high specificity and relatively-low cost, and is applicable to grassroots units.

The invention provides a rive CYP704B2 gene mutant, and applications thereof, and belongs to the technical field of gene engineering. According to the rive CYP704B2 gene mutant, indica rice 93-11 is subjected to cobalt 60 radiation-induced mutation so as to replace three G bases after the 794th base of rice CYP704B2 gene with one T base; the CYP704B2 gene mutant is named as cyp704B2-3; the nucleotide sequence of the CYP704B2 gene mutant is represented by SEQ ID No.1; it is further confirmed that rice recessive genic male sterility is induced by the CYP704B2 gene mutant; the CYP704B2 gene mutant can be used for culturing transgenic rice with recessive genic male sterility, and possesses great importance in rice genetic resource genetic improvement-breeding. The invention also provides a molecular marked identification method and applications of the rive CYP704B2 gene mutant in breeding.

Relating to the technical field of species identification, the invention specifically relates to a DNA barcoding standard gene sequence of Rizhao Blepharipoda liberata and species identification method based on the gene sequence. The DNA barcoding standard gene is COI gene, which has a gene sequence shown as SEQIDNO.1. The identification method includes: conducting extraction separation of DNA on a to-be-tested tissue, and amplifying a target gene by polymerase chain reaction. A pair of primers used in the amplification reaction are shown as the following: a forward primer 5'-GGTCAACAAATCATAAAGATATTGG-3', and a reverse primer 5'-TAAACTTCAGGGTGACCAAAAAATCA-3'. The gene sequence obtained in the invention is in favor of realizing molecular identification of the Rizhao Blepharipoda liberata, and can effectively shorten the identification time of the Rizhao Blepharipoda liberata.