Red yeast rice rich in Monacolin K and preparation method of red yeast rice
A kind of technology of red yeast rice and Monascus, applied in the red yeast rice rich in MonacolinK and the field of preparation thereof
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Embodiment 1
[0024] (1) Isolation of Monascus strains: Using PDA plate medium, Monascus strains were isolated from waste water and residues from different red yeast rice wine factories in Fujian Province, and commercially available red yeast rice and rice wine from different sources, and purified After culturing, 40 Monascus strains were obtained, and the purified strains were cultured on PDA slant medium.
[0025] (2) Screening of high-yielding MonacolinK strains: MonacolinK content and citrinin in the fermentation products of Monascus were used as indicators, and the fermented products of 40 Monacolin K strains were dried at 50°C to constant temperature. After weighting, it was ground into powder, and after necessary pretreatment, the contents of MonacolinK and citrinin were determined by HPLC. The results showed that the strain with the highest yield of MonacolinK and no citrinin production was FG-8.
[0026](3) Molecular identification of the Monacolin K strain: Genomic DNA of the myc...
Embodiment 2
[0029] (1) Preparation of Monascus seed liquid: After activating the Monascus strain on a PDA plate (28°C) for 5-7 days, use a puncher with a diameter of 5mm to take out the bacterial block, and then add 5 pieces of bacterial cake to the In a 250mL Erlenmeyer flask containing 50mL seed liquid culture medium (filled with small glass beads), three replicates were set up for each strain, and the seed liquid was obtained after shaking culture at 30°C and 150rpm for 72h.
[0030] (2) Preparation of rice culture medium: wash the rice, soak it in water for three hours, drain it and steam it in a steamer for 20 minutes (about 8 mature), weigh 50g of rice for each fermentation bottle, add 3wt% of Glucose, 4wt% NaNO 3 , 1wt% sodium acetate, after sealing, autoclave at 121°C for 20min.
[0031] (3) Inoculation and fermentation culture: Dilute the seed solution after 72 hours of cultivation to a spore concentration of approximately 1×10 7 1 / mL, according to 10% inoculum volume, add the ...
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