Microbial fermentation agent for cottonseed meal detoxification and preparation method and application thereof

A technology for microbial starter and cottonseed meal, applied in the field of starter, can solve the problems of high cost, unfavorable industrialized use, etc., and achieve the effects of low cost, reduction of fecal odor and harmful gas emissions, and easy preservation.

Inactive Publication Date: 2013-10-23
SHANGHAI CHUANGBO ECOLOGICAL ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] From the perspective of the existing technology, the fermentation of cottonseed cake and cottonseed meal generally adopts the compound fermentation of multiple

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] Step 1: Culture Saccharomyces cerevisiae (ACCC20065) on potato medium (PDA), subculture Bacillus subtilis (ACCC10619) on nutrient agar medium, observe the growth rate of the bacteria, and culture at 28°C for 36 hours; Saccharomyces cerevisiae colonies larger than 2mm and strains of Bacillus subtilis colonies with a diameter larger than 5mm are stored at 4°C for later use;

[0059] The second step: Inoculate the Saccharomyces cerevisiae strain obtained in the first step in potato medium (PDA), inoculate the Bacillus subtilis strain in nutrient agar medium, and inoculate the two strains at a constant temperature of 250 rpm Cultivate in a shaker at 28°C for 24 hours, take samples, and use the plate counting method to determine the total number of colonies. The strains with a total number of colonies greater than 2 billion / ml are used for seed production;

[0060] Step 3: Transfer the screened Bacillus subtilis and Saccharomyces cerevisiae into eggplant bottles equipped wit...

Embodiment 2

[0070] Step 1: Cultivate Saccharomyces cerevisiae (ACCC20065) on potato medium (PDA), subculture Bacillus subtilis (ACCC10619) on nutrient agar medium, observe the growth rate of the strain, and cultivate at 30°C for 36 hours to reduce the diameter of 2mm Saccharomyces cerevisiae colonies, strains of Bacillus subtilis colonies with a diameter greater than 5mm are preserved at 4°C;

[0071] The second step: Inoculate the Saccharomyces cerevisiae strain obtained in the first step in potato medium (PDA), inoculate the Bacillus subtilis strain in nutrient agar medium, and inoculate the two strains at a constant temperature of 250 rpm Cultivate in a shaker at 30°C for 24 hours, take samples, and use the plate counting method to determine the total number of colonies. The strains with a total number of colonies greater than 2 billion / ml are used for seed production;

[0072] Step 3: Transfer the screened Bacillus subtilis and Saccharomyces cerevisiae into eggplant bottles equipped w...

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Abstract

The invention discloses a microbial fermentation agent for cottonseed meal detoxification and a preparation method thereof. According to the preparation method, saccharomyces cerevisiae and bacillus subtilis serve as active strains and are subjected to expanding culture by using liquid fermentation, a carrier is added, and then, the microbial fermentation agent is prepared. According to the microbial fermentation agent, free gossypol in cottonseed meal can be degraded effectively, crude fibers are degraded and softened, the palatability of the cottonseed meal is improved, a large number of nutrition and hormone substances, such as active probiotics, bioactive small-peptide amino acids, various biochemical enzymes, somatomedins and the like, are secreted and synthesized, and the immunity of animals is effectively improved by microbial thalli protein, beta-glucan and the like in the microbial fermentation agent. The microbial fermentation agent further has the advantages that the strains and fermentation processes are simple, the cost is low, the consumption of the strains in cottonseed meal fermentation is low, the cost for the cottonseed meal fermentation is low, and the like.

Description

technical field [0001] The invention relates to a starter that can be used to produce animal feed raw materials, in particular to a starter that includes Saccharomyces cerevisiae and Bacillus subtilis, a preparation method thereof, and a method for treating cottonseed meal with the starter for detoxification. Background technique [0002] my country has a large population, and the production of grain and feed has always been a weak link in the national economy. Due to population growth, reduction of arable land, and increase in meat consumption, the balance of grain supply and demand is very fragile. The per capita grain has been below 400kg. Among them, the total grain output 40% of the feed is used for feed production, so the development of high-efficiency feed industry, the improvement of the conversion efficiency of grain to livestock products and the utilization rate of feed, and the development of new feed raw materials are to meet people's increasing demand for meat, po...

Claims

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Application Information

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IPC IPC(8): A23K1/00A23K1/14A23L1/015A23L5/20
Inventor 江瀚张玉辉
Owner SHANGHAI CHUANGBO ECOLOGICAL ENG
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