39 results about "Gossypol" patented technology

The invention provides a tobacco leaf nitrogen efficient sesame cake fertilizer and a preparation method thereof, and relates to the field of fertilizers. The nitrogen efficient sesame cake fertilizer for tobacco leaves comprises a solid material and water, the solid material comprises the following raw materials in percentage by mass: 60%-70% of sesame cake, 6%-8% of cottonseed cake, 12%-26% of rice husk and 8%-10% of polyaspartic acid, wherein the total mass of the raw materials is 100%. The nitrogen efficient sesame cake fertilizer for the tobacco leaves can effectively improve the quality of the tobacco leaves, improve the growth vigor of the tobacco leaves and improve the hacking degree of flue-cured tobacco leaves to a certain extent, and the cottonseed cake is added due to the fact that gossypol with a bactericidal effect is contained in the cottonseed cake, and the occurrence of soil diseases can be relieved or inhibited.

The present invention provides a composition for controlling the population of wild or feral pigs by reducing their fertilization rate. [Solution] An orally ingestible contraceptive composition for pigs, comprising 0.005 to 1.4% by weight of free gossypol; and a non-free gossypol substance comprising bulk feed components, pig attractants, flavor components, or any combination thereof.

The invention relates to a hybrid nano assembly for dual synergistic chemotherapy as well as a preparation method and application of the hybrid nano assembly, and belongs to the technical field of pharmaceutical preparations. The invention relates to a hybrid nano assembly for dual synergistic chemotherapy, which is formed by co-assembling a cabazitaxel prodrug and gossypol under hydrophobic action and hydrogen bond driving, and is modified by adopting a PEG (Polyethylene Glycol) modifier, the molar ratio of the cabazitaxel prodrug to the gossypol is 1: (1-7), and the ratio of the total mass of the cabazitaxel prodrug and the gossypol to the mass of the PEG modifier is 4: 1. A cabazitaxel prodrug and a sensitizer gossypol are co-assembled to obtain a hybrid nano assembly, the hybrid nano assembly is oxygenated to obtain the oxygen-carrying preparation, the nano assembly and the oxygen-carrying preparation are simple and convenient to prepare and high in drug loading efficiency, and efficient co-loading of drugs can be achieved; meanwhile, the curative effect of chemotherapy can be enhanced through dual mechanisms of relieving tumor hypoxia and promoting tumor apoptosis, and the pharmaceutical composition has a good clinical application prospect.

The present application belongs to the technical field of microbial fermentation feed, and discloses a fattening sheep complete mixed granular feed fermented by lactobacillus paracasei and a preparation method. The present application provides a lactobacillus paracasei strain for degrading gossypol, and the strain is used for solid fermentation of a mixture of cottonseed meal and cassava powder to obtain a fermented feed. The fermented feed is used for preparing a fattening sheep complete mixed granular feed, which can be directly used for feeding fattening sheep and can completely meet the nutritional needs of the fattening sheep. The complete mixed granular feed can significantly improve the palatability, and increase the feed intake and daily weight gain of the fattening sheep through fermentation of the cottonseed meal and the cassava powder.

The invention discloses a non-grain type protein source feed quality safety evaluation method, and particularly relates to the technical field of feed detection. The method comprises the following steps: obtaining a non-grain type protein source feed sample, drying, crushing and homogenizing; detecting protein content and target amino acid composition, and constructing a nutrition suitability scoring model; detecting anti-nutritional factors such as lignin, tannin and gossypol, and calculating an anti-nutritional risk score based on a limit value; detecting the content of heavy metals such as lead, cadmium, mercury and arsenic, and generating a heavy metal safety score; detecting the content of biotoxins such as aflatoxin, vomitoxin and zearalenone, and outputting a biotoxin safety score; and finally, performing weighted calculation on each score, and constructing a comprehensive quality safety index model to obtain the quality safety level of the feed. The method realizes multi-dimensional and quantitative evaluation of the nutrition and safety of the non-grain type protein source feed, is suitable for feed resource development, alternative raw material screening and feed formula optimization, and has the advantages of strong adaptability and strong operability.

The invention discloses a method for removing free gossypol in cottonseed protein by using an ultrasonic-assisted eutectic solvent. According to the method, a deep eutectic solvent composed of choline chloride and malonic acid according to the molar ratio of 1: 2 is adopted as an extracting agent, ultrasonic extraction is combined, gossypol in the cottonseed protein is efficiently removed, the gossypol residual quantity of the prepared dephenolized cottonseed protein is smaller than or equal to 20 mg / kg, raw material waste is reduced, cost is reduced, and large-scale industrial production is powerfully supported.

The utility model relates to the technical field of gamma -position decanoin crude product preparation technology discloses a kind of feeding devices for gamma -position decanoin crude product preparation, including feeding cylinder, the front of feeding cylinder is provided with controller, the outer wall of feeding cylinder is uniformly provided with support leg, the top of feeding cylinder is provided with feeding assembly, the feeding assembly includes: mixing component, setting at the top of feeding cylinder;Cleaning component, setting at one side of feeding cylinder;The mixing component includes: mixing cylinder, is connected and is set at the top of feeding cylinder;Motor, setting at the top of mixing cylinder.The utility model is pumped to the cleaning fluid in liquid storage tank is sprayed to the inside of feeding cylinder through connecting box and spray head, and the filter plate is conveniently extracted to clean or replace by mounting plate, these designs not only facilitate the daily cleaning maintenance of equipment, but also can reduce the corrosion of raw material residue to equipment, prolong the service life of equipment.

The invention discloses bacillus subtilis aldehyde dehydrogenase Dhas and application of the bacillus subtilis aldehyde dehydrogenase Dhas in degradation of gossypol. The nucleotide sequence of the gene is shown as SEQ ID No: 1 in a sequence table. The Dhas enzyme provided by the invention has extremely high affinity and catalytic efficiency on a substrate gossypol, and the degradation efficiency of the Dhas enzyme is far higher than that of other gossypol degrading enzymes reported at present. The method is good in stability, high in safety and low in application cost, protects nutritional ingredients, is high in enzymatic treatment specificity, does not damage nutritional substances such as protein and amino acid in the cottonseed meal, and remarkably improves the feeding value of the cottonseed meal.

The application belongs to the technical field of chemical industry, and particularly relates to a cottonseed dephenolized concentrated protein extraction equipment and method, which comprises pretreatment unit, extraction unit, post-treatment unit, evaporation and concentration unit and solvent recovery unit connected in sequence. The pretreatment unit comprises double roller embryo press, bulking machine and turnover plate cooler. The extraction unit comprises extractor A for removing oil and fat by n-hexane extraction and extractor B for removing gossypol by methanol extraction. The post-treatment unit comprises horizontal screw conveyor dryer A and horizontal screw conveyor dryer B for drying and desolventizing the extracted cottonseed meal to obtain cottonseed dephenolized concentrated protein. The evaporation and concentration unit is used for evaporating and concentrating the mixed oil and mixed liquid after extraction. The solvent recovery unit comprises multiple condensers connected with the evaporation and concentration unit. The application can realize low-temperature and high-efficiency extraction. The protein content of the extracted product cottonseed dephenolized concentrated protein is as high as 72.7-76.2%, the gossypol content is as low as 230-290 ppm, the residual solvent in the protein is as low as 280-340 ppm, and the product quality is high.

The invention provides a gossypol specific nucleic acid aptamer as well as a preparation method and application thereof. The nucleotide sequence of the aptamer is shown as SEQ ID NO. 1. A Capture-SELEX technology is adopted, a screening system is optimized, high-throughput sequencing and DNAMAN homology and secondary structure analysis are combined, the gossypol specific nucleic acid aptamer is obtained, ThT fluorescence determination and ITC verification show that the aptamer still keeps high affinity after truncation, and the gossypol specific nucleic acid aptamer can be used for preparing the gossypol specific nucleic acid aptamer. Molecular docking further defines that key basic groups such as G-26, C-27, G-28 and G-46 form a hydrogen bond-hydrophobic effect with gossypol, and a foundation is laid for construction of a subsequent gossypol rapid detection system.

The application belongs to the technical field of animal feed, and particularly relates to an egg-laying chicken feed without soybean meal, and a preparation method and application thereof. The method comprises the following steps: S1, mixing wheat flour, barley flour, cottonseed meal and glutamic acid residue, crushing the granules to obtain broken material; and S2, mixing the broken material with corn, sunflower meal, corn gluten meal, pepper residue, granular stone powder, calcium hydrogen phosphate, soybean oil, compound enzyme, sodium chloride, sodium bicarbonate, egg methionine, lysine sulfate and threonine to obtain the egg-laying chicken feed without soybean meal. The application provides comprehensive essential amino acids, especially limiting amino acids such as lysine, egg methionine and threonine, by the synergistic combination of cottonseed meal, sunflower meal, corn gluten meal and glutamic acid residue, so as to meet the nutritional requirements of egg-laying chickens. The application granulates at a temperature of 75-85 DEG C, effectively degrades free gossypol in the cottonseed meal, reduces the content of free gossypol to less than 30 mg / kg in the finished feed, and eliminates the adverse effects on the health of egg-laying chickens.

The invention relates to the technical field of biology, in particular to an MT molecular marker HapII combination for detecting high-content solanine and application of the MT molecular marker HapII combination. MT molecular markers HapII-1 and HapII-2 which are remarkably related to the solanine content of potatoes are obtained, the molecular markers are used for detecting a potato breeding material, and the content of solanine in the potato breeding material is detected. The method can accurately and efficiently predict whether the solanine content in the potato tubers reaches 200 mg / kg or not under the condition that planting is not needed to wait for flowering and fruiting of potato plants, can efficiently assist in potato breeding selection, is a novel breeding mode which is time-saving and high in accuracy, and has extremely high economic value.

Aiming at the problems of incomplete kernel-hull separation, low oil extraction rate, standard exceeding of free gossypol and the like in the traditional process, the invention provides a whole-process collaborative optimization process, which comprises the following steps: (1) performing three-stage screening and wind power seed cleaning to ensure that the impurity content of clean cottonseeds is less than or equal to 0.5%; (2) a tooth roller type husking machine is matched with a double-layer vibrating screen and a backflow re-husking system, the husking efficiency is larger than or equal to 95%, the shell content in kernels is adjustable in 15-18%, the kernel content in shells is smaller than or equal to 0.5%, and the cotton kernel breakage rate is smaller than or equal to 3%; (3) the flaking thickness ranges from 0.3 mm to 0.4 mm; (4) the steaming and frying temperature is 100-105 DEG C, and the water content is 8-10%; (5) the oil content of the pre-pressed cake is not more than 14%; (6) the solvent leaching temperature is 40-45 DEG C, and the solvent ratio is 1: (1.2-1.5); (7) crude protein of the cottonseed meal finished product is greater than or equal to 42% and free gossypol is less than or equal to 0.02%. The PLC intelligent control system is adopted to realize parameter linkage regulation and control, the solvent recovery rate is greater than or equal to 99.5%, the unit steam consumption is less than or equal to 220kg / t cake, efficient, low-consumption and high-quality continuous production is realized, and the comprehensive utilization rate of cotton seeds and the added value of products are remarkably improved.

The invention relates to the technical field of plant genetic engineering and crop breeding, in particular to a method for creating specific low-gossypol-content cotton resistant to aphids and thrips, a cis-regulatory element site for regulating seed gland and plant epidermal hair development is identified through epidermal regulatory omics analysis, and a cis-regulatory element site for regulating seed gland and plant epidermal hair development is established by adopting a CRISPR / Cas9 genome editing technology. The cis-regulation element site is directionally deleted or edited and modified, a cotton material with extremely low gossypol content in seeds and normal gossypol content in stalks and leaves can be created, and meanwhile, the epidermal hair density of plants is remarkably improved, so that the plants show relatively strong aphid and thrip resistance. The cis-regulation site has the function of specifically regulating seed gland and plant epidermal hair development, provides a new site for creation and breeding improvement of insect-resistant and low-phenol cotton materials, and can be used for creation of insect-resistant low-phenol new materials or genetic improvement of existing materials after gene editing. Good breeding utilization and market development prospects are realized.

The present application relates to a kind of high conversion rate degradation cotton stalk free gossypol enzyme combination, in view of the existing cotton stalk free gossypol strong toxicity, low degradation efficiency, lead to its resource utilization is limited, the present application obtains efficient degradation strain and compound specific enzyme preparation, constructs and forms bacteria enzyme combination system.The bacteria enzyme combination includes degradation strain fermentation liquor and composite enzyme preparation, wherein the degradation strain is the mixed strain of pseudomonas and bacillus, and the composite enzyme preparation is compounded by laccase, peroxidase and esterase according to a specific ratio.The degradation rate of the combination to free gossypol in cotton stalk can reach more than 95%, which is significantly better than the degradation effect of single strain or single enzyme preparation.

This invention belongs to the field of feed processing technology and provides a compound bacterial seed liquid for solid-state fermentation of small peptide cottonseed meal feed, and a method for preparing small peptide cottonseed meal feed using this seed liquid. This invention uses a mixture of Aspergillus niger, Aspergillus oryzae, and Saccharomyces cerevisiae seed liquids in a volume ratio of 1:3:1, wherein the viable cell concentrations of Aspergillus niger, Aspergillus oryzae, and Saccharomyces cerevisiae are 1.0×10¹¹ CFU / mL, 1.1×10¹¹ CFU / mL, and 1.2×10¹¹ CFU / mL, respectively. Cottonseed meal is used as the substrate, with the addition of ammonium chloride and potassium dihydrogen phosphate, and fermented under constant temperature. The fermented cottonseed meal prepared by this invention is characterized by high protein, high small peptide content, and zero gossypol, which can significantly improve animal growth performance and provide a feasible solution for the high-value application of cottonseed meal in the feed industry.

This utility model relates to the technical field of delinting equipment and discloses a delinting device for processing high-protein cottonseed meal, including a shell, a hopper, a delinting roller, an upper protective shell, a lower protective shell, a grinding groove, a grinding disc, a sanding disc, a delinting groove, a guide cover, a discharge cover, a bushing, a connecting shaft, a cleaning brush, a motor, a rotating shaft, a first drive belt, and a second drive belt. In this utility model, cottonseed material can be fed into the space between the protective shell and the lower protective shell through the hopper. At this time, the delinting roller can drive the falling cottonseed to rotate and roll through the grinding groove, and the fluff on the surface of the cottonseed can be delinted by the friction between the grinding disc and the sanding disc. When the cottonseed moves to the position of the guide cover, it can be discharged through the discharge cover. After the motor is started, it can drive the first drive belt to rotate through the rotating shaft, so that multiple sets of connecting shafts inside the discharge cover rotate in linkage. The cleaning brush can then rub and clean the falling cottonseed again, improving the delinting effect of the cottonseed, avoiding multiple feedings, and thus improving processing efficiency.

The invention belongs to the technical field of high-value utilization of agricultural product processing byproducts, and discloses compound bacteria for degrading free gossypol in cottonseed molasses and a fermentation process of the compound bacteria. The compound bacteria are prepared from aspergillus niger, saccharomyces cerevisiae and lactobacillus gadsenii according to a bacterial liquid volume ratio of 1: 2: 1; the fermentation process parameters are as follows: the inoculum size is 5%, the fermentation temperature is 27.8 DEG C, and the fermentation time is 8.4 days. According to the scheme, the degradation rate of free gossypol in the cottonseed molasses reaches 72% and is increased by 6% compared with the degradation rate of single bacteria; meanwhile, metabolic pathways of ABC transport protein, vitamin absorption and the like can be regulated and controlled, the metabolite composition of the product is changed, the nutritional value and safety of the product are improved, and the method can be used for preparing feed additives or food raw materials.

This invention discloses a highly efficient yeast strain ZYS-3 that degrades gossypol and its applications, belonging to the fields of fermentation engineering and livestock feed technology. Using strain ZYS-3, belonging to the *Saccharomyces cerevisiae*, and adding a gossypol adsorbent during fermentation, the gossypol degradation rate reaches 95.23%. This gossypol adsorbent uses biomass porous charcoal as a matrix, and lysine is grafted onto the surface of the biomass porous charcoal using an epoxy silane coupling agent. On the one hand, this reduces the loss of lysine during the substrate humidification process, ensuring the adsorption effect on free gossypol; on the other hand, it utilizes the adsorption function of biomass porous charcoal to enhance the enrichment of free gossypol in cottonseed meal, accelerating the binding of the phenolic hydroxyl groups of free gossypol to the ε-amino groups of lysine to form bound gossypol, reducing the inhibitory effect of free gossypol on the strain, and further improving fermentation efficiency.

The present application relates to the technical fields of biological medicine and functional biological material, and particularly relates to an injectable composite hydrogel, a preparation method thereof and application thereof in regulating lactic acid metabolism and remodeling tumor immune microenvironment. The injectable composite hydrogel is prepared by synthesizing copper-gossypol complex through coordination reaction of soluble divalent copper salt and ligand gossypol in a solution system, and then dispersing the copper-gossypol complex uniformly in a sodium magnesium lithium silicate hydrogel network to form a composite system. The preparation method is simple, the component content can be precisely controlled, the preparation conditions are mild and controllable, the energy consumption is low, and the prepared injectable composite hydrogel can target and regulate lactic acid metabolism of osteosarcoma, remodel tumor immune microenvironment, promote bone tissue repair, and effectively induce immunogenic death of tumor cells, thereby effectively activating the body's anti-tumor immune response. The application of the injectable composite hydrogel in the preparation of a drug for treating osteosarcoma has a good application prospect.

The application discloses application of methylated gossypol in preparation of a coronavirus inhibitor. The application provides application of at least one of a compound as shown in a formula I, a solvate of the compound, a pharmaceutically acceptable salt of the compound, a solvate of the pharmaceutically acceptable salt of the compound in preparation of the coronavirus inhibitor. The compound provided by the application has the advantages of clear target, high safety, significantly improved inhibitory activity, potential for wide application and potential therapeutic effect on diseases caused by the novel coronavirus.

This invention discloses a podophyllotoxin-9-fluorenylmethanol small molecule prodrug nanoparticle, its preparation method, and its applications. The podophyllotoxin-9-fluorenylmethanol small molecule prodrug co-assembled nanoparticles are either polyethylene glycol-modified podophyllotoxin-9-fluorenylmethanol small molecule prodrug co-assembled with gossypol, or podophyllotoxin-9-fluorenylmethanol small molecule prodrug co-assembled with gossypol and loaded with hydrophobic fluorescent substances. The podophyllotoxin-9-fluorenylmethanol small molecule prodrug is prepared by linking podophyllotoxin and its compounds with 9-fluorenylmethanol via disulfide bonds. This invention is the first to construct a carrier-free hybrid nanoassembly of gossypol and PPT oxidation-sensitive prodrug. Gossypol-mediated prodrug co-assembled nanoparticles can be used for precision cancer chemotherapy in tumor treatment, precisely achieving PPT "enhancement," improving the therapeutic efficiency of PPT prodrugs, opening an ultra-low-dose chemotherapy window for PPT, and solving the problems of delayed and insufficient prodrug activation.

The invention belongs to the technical field of edible oil and fat refining, and particularly relates to modified chlorella powder, a preparation method thereof and application of the modified chlorella powder in cottonseed oil dephenolization. After characterization, the apparent morphology is obviously changed, the reaction activity is improved, the success of CTAB grafting is proved, the adsorption rate of CTAB-ACP to gossypol reaches 90.80%, and the adsorption quantity is 67.55 mg / g; cTAB-ACP has physical and chemical adsorption effects on gossypol adsorption at the same time, and adsorption driving force is electrostatic attraction, hydrophobic interaction, hydrogen bonds and pi-pi complexation; quality analysis shows that free fatty acid in the cottonseed oil is removed after adsorption, the oxidative rancidity degree is reduced, and the oil stability is improved; important sterol active substances are not obviously changed, and the VE content is slightly reduced; the activity of GES-1 cells after co-culture exceeds 80%; the CTAB-ACP prepared in the invention has the advantages of high adsorption capacity, low cost and high biological safety, and has a wide application prospect.

A method for evaluating an influence by gene-edited low-gossypol cotton on Aphis gossypii is provided, relating to the technical field of cotton safety evaluation. The method for evaluating an influence by gene-edited low-gossypol cotton on Aphis gossypii includes planting the gene-edited low-gossypol cotton indoors, collecting young leaves and inoculating nymphal Aphis gossypii on the leaves, rearing the nymphal Aphis gossypii, recording a number of adult Aphis gossypii deaths and a number of newly hatched nymphal Aphis gossypii, calculating an Aphis gossypii mortality rate and an average Aphis gossypii productivity, and evaluating the influence.

The invention discloses a preparation method of high-resveratrol peanut oil, and belongs to the technical field of edible oil processing. The method comprises the following steps: pretreating peanuts and peanut root powder; the solubility saturation value of resveratrol in squeezed crude oil is measured, the target content is set, and the adding proportion of the peanut root powder is accurately determined by measuring the resveratrol dissolution concentration of the treated peanut root powder under a preset extraction process and combining the residual oil mass of the peanut cake meal; mixing the peanut cake meal with the treated peanut root powder in proportion to obtain a mixed raw material; carrying out extraction operation of at least two polarity increasing stages on the mixed raw material; and finally, carrying out solid-liquid separation and desolventizing treatment on the leached mixture to obtain the high-resveratrol peanut oil. According to the method, the resveratrol is efficiently and stably enriched in the peanut oil through accurate material ratio and gradient extraction, and technical support is provided for development of high-value-added functional peanut oil products.

The invention relates to glycosyltransferase for catalyzing glycosylation of hydroxyl groups at No.7 and No.8 sites of gossyn and application of the glycosyltransferase, and belongs to the technical field of enzyme engineering. The invention aims to excavate key glycosyl transferase UGT capable of catalyzing gossypol glycosylation, and provides a saccharomyces cerevisiae engineering bacterium capable of synthesizing gossypol glycoside derivatives by taking quercetin as a substrate, especially 7 # and 8 # hydroxyl glycosylated derivatives. Specifically, the glucoside transferase for efficiently synthesizing the gossydin-7-O-glucoside and the gossydin-8-O-glucoside is determined by performing tissue collection, RNA (Ribonucleic Acid) extraction, gene amplification and bioinformatics analysis, preliminary verification of cotton cotyledons and fermentation verification of saccharomyces cerevisiae on various plant materials rich in flavone. And then integrating F8H hydroxylase required by cotton element biosynthesis and UGT with a specific catalytic function in the saccharomyces cerevisiae to construct the saccharomyces cerevisiae engineering bacterium synthesized by the cotton element derivative.

This invention relates to the fields of biomedicine and functional biomaterials, specifically to an injectable composite hydrogel, its preparation method, and its application in regulating lactate metabolism and remodeling the tumor immune microenvironment. The injectable composite hydrogel is prepared by synthesizing a copper-gossypol complex through a coordination reaction between a soluble divalent copper salt and the ligand gossypol in a solution system. This copper-gossypol complex is then uniformly dispersed in a sodium magnesium lithium silicate hydrogel network to form a complex system. This preparation method is simple, allows for precise control of component content, employs mild and controllable preparation conditions, and has low energy consumption. The resulting injectable composite hydrogel can target and regulate lactate metabolism in osteosarcoma, remodel the tumor immune microenvironment, promote bone tissue repair, and effectively induce immunogenic cell death in tumor cells, thereby effectively activating the body's anti-tumor immune response. The application of this injectable composite hydrogel in the preparation of drugs for the treatment of osteosarcoma shows great promise.

The invention relates to the technical field of insulating oil, and particularly discloses a decolorizing method for preparing cottonseed-based natural ester insulating oil, which comprises the following steps: S1, taking degummed and deacidified cottonseed oil as raw material oil, adding a molecular sieve, mixing under a heating vacuum condition, and then carrying out solid-liquid separation to obtain pretreated oil; and S2, mixing the pretreated oil obtained in the step S1 with acidified activated clay, mixing and decoloring at 95-105 DEG C under a vacuum condition, and then carrying out solid-liquid separation to finish decoloring treatment. The method comprises the following steps: firstly, carrying out targeted pre-adsorption on degummed and deacidified cottonseed oil by adopting a Fe-Zr bimetallic modified 13X molecular sieve to remove gossypol and trace metal ions; afterwards, the acidified activated clay is used for synergistic decoloration, the use amount of the activated clay in decoloration treatment can be remarkably reduced, and the residual amount of gossypol and the content of trace metal ions in the prepared insulating oil are low.

This invention relates to an iron polyphenol nanocapsule, its preparation method, and its applications. The nanocapsule is prepared from raw materials containing ferric salts, ferric ions, and gossypol via a microfluidic chip. The iron polyphenol nanocapsule containing gossypol-ferric ions-ferric ions prepared by this invention using a microfluidic chip as a reactor exhibits uniform size and good stability. It can achieve co-delivery of dual chemotherapy drugs, T1 magnetic resonance imaging, and trimodal combined therapy of tumors (chemodynamic therapy / dual-channel chemotherapy / immunotherapy), showing potential applications in cancer diagnosis and treatment.