76 results about "Gossypol" patented technology

The invention relates to the technical field of insulating oil purification, and particularly discloses a deep purification and performance improvement method of vegetable insulating oil, which comprises the following steps: determining fluorination parameters through molecular dynamics simulation, carrying out surface fluorination modification on a polypropylene filter membrane, and constructing a high-coverage C-F bond layer; specific endogenous impurities such as gossypol, phospholipid and pigment in the vegetable insulating oil such as cotton seeds can be effectively removed, and the problems of oxidation acceleration, insulation performance reduction and equipment aging caused by the specific endogenous impurities are avoided; and a core technical support is provided for long-acting stable operation of the vegetable insulating oil.

The invention provides a tobacco leaf nitrogen efficient sesame cake fertilizer and a preparation method thereof, and relates to the field of fertilizers. The nitrogen efficient sesame cake fertilizer for tobacco leaves comprises a solid material and water, the solid material comprises the following raw materials in percentage by mass: 60%-70% of sesame cake, 6%-8% of cottonseed cake, 12%-26% of rice husk and 8%-10% of polyaspartic acid, wherein the total mass of the raw materials is 100%. The nitrogen efficient sesame cake fertilizer for the tobacco leaves can effectively improve the quality of the tobacco leaves, improve the growth vigor of the tobacco leaves and improve the hacking degree of flue-cured tobacco leaves to a certain extent, and the cottonseed cake is added due to the fact that gossypol with a bactericidal effect is contained in the cottonseed cake, and the occurrence of soil diseases can be relieved or inhibited.

The present invention provides a composition for controlling the population of wild or feral pigs by reducing their fertilization rate. [Solution] An orally ingestible contraceptive composition for pigs, comprising 0.005 to 1.4% by weight of free gossypol; and a non-free gossypol substance comprising bulk feed components, pig attractants, flavor components, or any combination thereof.

The invention relates to a hybrid nano assembly for dual synergistic chemotherapy as well as a preparation method and application of the hybrid nano assembly, and belongs to the technical field of pharmaceutical preparations. The invention relates to a hybrid nano assembly for dual synergistic chemotherapy, which is formed by co-assembling a cabazitaxel prodrug and gossypol under hydrophobic action and hydrogen bond driving, and is modified by adopting a PEG (Polyethylene Glycol) modifier, the molar ratio of the cabazitaxel prodrug to the gossypol is 1: (1-7), and the ratio of the total mass of the cabazitaxel prodrug and the gossypol to the mass of the PEG modifier is 4: 1. A cabazitaxel prodrug and a sensitizer gossypol are co-assembled to obtain a hybrid nano assembly, the hybrid nano assembly is oxygenated to obtain the oxygen-carrying preparation, the nano assembly and the oxygen-carrying preparation are simple and convenient to prepare and high in drug loading efficiency, and efficient co-loading of drugs can be achieved; meanwhile, the curative effect of chemotherapy can be enhanced through dual mechanisms of relieving tumor hypoxia and promoting tumor apoptosis, and the pharmaceutical composition has a good clinical application prospect.

The present application belongs to the technical field of microbial fermentation feed, and discloses a fattening sheep complete mixed granular feed fermented by lactobacillus paracasei and a preparation method. The present application provides a lactobacillus paracasei strain for degrading gossypol, and the strain is used for solid fermentation of a mixture of cottonseed meal and cassava powder to obtain a fermented feed. The fermented feed is used for preparing a fattening sheep complete mixed granular feed, which can be directly used for feeding fattening sheep and can completely meet the nutritional needs of the fattening sheep. The complete mixed granular feed can significantly improve the palatability, and increase the feed intake and daily weight gain of the fattening sheep through fermentation of the cottonseed meal and the cassava powder.

The invention discloses a (-)-gossypol derivative, a preparation method thereof and an antioxidant, and relates to the technical field of chemical synthesis, the (-)-gossypol derivative comprises a (-)-gossypol-polylysine derivative and / or a pharmaceutically acceptable salt thereof, the repetitive structural unit of the (-)-gossypol-polylysine derivative comprises any one of a structural formula (I) and a structural formula (II); wherein n is equal to 25 to 40. In the novel compound synthesized by the invention, an aldehyde group in the (-)-gossypol and an amino group in the polylysine form a Schiff base compound through an imine bond, an active aldehyde group in the (-)-gossypol is closed, and meanwhile, the (-)-gossypol-polylysine derivative has relatively strong antioxidant activity and is a relatively good antioxidant and drug source.

The invention relates to a method for improving pigment gland characters and gossypol characters of cotton seeds and application of the method. A cotton seed specific promoter is used for specifically inhibiting expression and / or activity of Gl2 / Gl3 protein in the seed development process, novel cotton with seeds free of pigment glands and normal plant pigment glands can be obtained, the gossypol content of the seeds is zero, and the gossypol content of other tissues is normal.

The invention belongs to the field of fluorescent probes, discloses a supramolecular fluorescent probe for rapid detection of gossypol as well as a preparation method and application of the supramolecular fluorescent probe, and solves the technical problems of low gossypol content detection accuracy, long response time and environmental pollution easily caused by use of an organic solvent or strong acid in an existing technology for detecting gossypol by a fluorescence method. The supramolecular fluorescent probe for gossypol detection is formed by combining human serum albumin and a probe P2, the structural formula of the probe P2 is as follows: # imgabs0 # P2 and HSA are combined to form a supramolecular compound, and rapid detection of gossypol concentration in a water phase is realized through self object-object interaction of supramolecules. The fluorescence intensity of P2 at HSA and the gossypol concentration (0-8 [mu] M) have a good linear relationship, R2 is equal to 0.9912, the detection limit is 0.0137 [mu] M, and a precious thought and a new strategy are provided for subsequent development of gossypol response type fluorescent probes.

The invention provides application of gossypol in preparation of chicken feed or medicine for preventing and treating chicken coccidiosis, and relates to the technical field of parasitic disease prevention and treatment. Experimental exploration proves that gossypol has a new application of preventing and treating chicken coccidiosis, a new scheme is provided for development of drugs and feeds for preventing and treating chicken coccidiosis, a new idea is provided for clinical control of chicken coccidiosis, and the application range of gossypol is expanded. Gossypol acts on chicks infected with chicken eimeria tenella disease, compared with chicks in a challenge control group, the reduction rate of oocyst discharge of chicks in a gossypol treatment group reaches 82.5%, the relative weight gain rate can reach 90.8%, the coccidiosis resistance index of the chicks can reach 167.4, and bleeding symptoms caused by chicken coccidiosis can be effectively reduced.

The invention discloses a non-grain type protein source feed quality safety evaluation method, and particularly relates to the technical field of feed detection. The method comprises the following steps: obtaining a non-grain type protein source feed sample, drying, crushing and homogenizing; detecting protein content and target amino acid composition, and constructing a nutrition suitability scoring model; detecting anti-nutritional factors such as lignin, tannin and gossypol, and calculating an anti-nutritional risk score based on a limit value; detecting the content of heavy metals such as lead, cadmium, mercury and arsenic, and generating a heavy metal safety score; detecting the content of biotoxins such as aflatoxin, vomitoxin and zearalenone, and outputting a biotoxin safety score; and finally, performing weighted calculation on each score, and constructing a comprehensive quality safety index model to obtain the quality safety level of the feed. The method realizes multi-dimensional and quantitative evaluation of the nutrition and safety of the non-grain type protein source feed, is suitable for feed resource development, alternative raw material screening and feed formula optimization, and has the advantages of strong adaptability and strong operability.

The invention discloses a method for removing free gossypol in cottonseed protein by using an ultrasonic-assisted eutectic solvent. According to the method, a deep eutectic solvent composed of choline chloride and malonic acid according to the molar ratio of 1: 2 is adopted as an extracting agent, ultrasonic extraction is combined, gossypol in the cottonseed protein is efficiently removed, the gossypol residual quantity of the prepared dephenolized cottonseed protein is smaller than or equal to 20 mg / kg, raw material waste is reduced, cost is reduced, and large-scale industrial production is powerfully supported.

The utility model relates to the technical field of gamma -position decanoin crude product preparation technology discloses a kind of feeding devices for gamma -position decanoin crude product preparation, including feeding cylinder, the front of feeding cylinder is provided with controller, the outer wall of feeding cylinder is uniformly provided with support leg, the top of feeding cylinder is provided with feeding assembly, the feeding assembly includes: mixing component, setting at the top of feeding cylinder;Cleaning component, setting at one side of feeding cylinder;The mixing component includes: mixing cylinder, is connected and is set at the top of feeding cylinder;Motor, setting at the top of mixing cylinder.The utility model is pumped to the cleaning fluid in liquid storage tank is sprayed to the inside of feeding cylinder through connecting box and spray head, and the filter plate is conveniently extracted to clean or replace by mounting plate, these designs not only facilitate the daily cleaning maintenance of equipment, but also can reduce the corrosion of raw material residue to equipment, prolong the service life of equipment.

The invention discloses streptomyces MB-1 and application thereof in cotton seed hull degradation, and belongs to the technical field of biology. The classification name of the streptomycete MB-1 is streptomycete sp, and the streptomycete MB-1 is sent to the China General Microbiological Culture Collection Center (CGMCC), the preservation number is CGMCC No.32420, and the preservation date is October 30, 2024. The streptomyces MB-1 disclosed by the invention is beneficial to realizing efficient bioconversion of cottonseed hull wastes rich in lignocellulose, the degradation rate of cottonseed hulls is 49.54%, the degradation rate of gossypol is 25.82%, a technical basis is provided for realizing resource application of agricultural wastes, and the development of circular agriculture is assisted.

The invention discloses a method for reducing gossypol content in cottonseed meal, raw materials are composed of cottonseed meal, and microbial fermentation probiotics are composed of pHY300PLK-Z recombinant bacillus subtilis. According to the method, the gossypol content in the cottonseed meal is reduced in an efficient, environment-friendly and low-cost manner, and the safety and nutritional value of the cottonseed meal as a feed raw material are improved.

The invention discloses a method for producing astaxanthin in cotton BKT Gene and application, belonging to the field of plant genetic engineering technology. In cotton, the enhanced constitutive 2×35S promoter is used to drive the tobacco Rubisco signal peptide to induce BKT The gene is expressed in cotton chloroplasts, successfully establishing an astaxanthin biosynthetic pathway, enabling astaxanthin production in various cotton tissues, including leaves, stems, anthers, sepals, and seeds. Testing of transgenic cotton seeds revealed a significant increase in weight and size, but a decrease in gossypol content. Therefore, the method of the present invention can create transgenic cotton plants rich in astaxanthin, with larger and heavier cotton seeds, lower gossypol content, and a more nutritious and less toxic profile. This method can be used to create cotton plants that are bio-factories for astaxanthin production, providing a stable, low-cost, and effective alternative to traditional astaxanthin sources.

The invention discloses bacillus subtilis aldehyde dehydrogenase Dhas and application of the bacillus subtilis aldehyde dehydrogenase Dhas in degradation of gossypol. The nucleotide sequence of the gene is shown as SEQ ID No: 1 in a sequence table. The Dhas enzyme provided by the invention has extremely high affinity and catalytic efficiency on a substrate gossypol, and the degradation efficiency of the Dhas enzyme is far higher than that of other gossypol degrading enzymes reported at present. The method is good in stability, high in safety and low in application cost, protects nutritional ingredients, is high in enzymatic treatment specificity, does not damage nutritional substances such as protein and amino acid in the cottonseed meal, and remarkably improves the feeding value of the cottonseed meal.

The invention discloses preparation and application of a supramolecular nano-micelle based on gossypol (GP). A preparation method comprises the following steps: (1) preparing a polymer PGP; (2) preparation of a polymer PGPP; and (3) preparing the PGPP supramolecular nano-micelle. The prepared micelle has the advantages of stable particle size, uniform dispersion, drug controlled release behavior, high drug loading capacity, low toxic and side effects, good biological safety and the like. The gossypol drug belonging to polyphenol has a certain anti-cancer effect but has toxic and side effects, and research shows that the toxic and side effects of gossypol on normal cells are reduced by modifying the aldehyde group on gossypol, and meanwhile, the gossypol drug has a certain anti-cancer effect but has toxic and side effects. The supramolecular nano-micelle is triggered by glutathione (GSH) and a pH microenvironment in cancer cells to realize double-response controllable release, so that gossypol is effectively enriched at a tumor site, the cancer cells are killed, and a new strategy and a systematic theoretical support are provided for development of a supramolecular prodrug delivery system.

The present invention relates to the field of genetic engineering technology, and particularly relates to a Gossypium hirsutum GhbHLH147 gene and its application in regulating gossypol synthesis and / or growth and development of cotton. The nucleotide sequence of the Gossypium hirsutum GhbHLH147 gene is shown in SEQ ID No.1. GhbHLH147 silencing results in a significant decrease in the expression levels of gossypol synthesis genes CDNC , CYP706B1 , CYP82D113 , DH‑1 , CYP71BE79 , and the content of free gossypol in cotton leaves also decreases significantly, indicating that GhbHLH147 may positively regulate gossypol synthesis.

The application belongs to the technical field of chemical industry, and particularly relates to a cottonseed dephenolized concentrated protein extraction equipment and method, which comprises pretreatment unit, extraction unit, post-treatment unit, evaporation and concentration unit and solvent recovery unit connected in sequence. The pretreatment unit comprises double roller embryo press, bulking machine and turnover plate cooler. The extraction unit comprises extractor A for removing oil and fat by n-hexane extraction and extractor B for removing gossypol by methanol extraction. The post-treatment unit comprises horizontal screw conveyor dryer A and horizontal screw conveyor dryer B for drying and desolventizing the extracted cottonseed meal to obtain cottonseed dephenolized concentrated protein. The evaporation and concentration unit is used for evaporating and concentrating the mixed oil and mixed liquid after extraction. The solvent recovery unit comprises multiple condensers connected with the evaporation and concentration unit. The application can realize low-temperature and high-efficiency extraction. The protein content of the extracted product cottonseed dephenolized concentrated protein is as high as 72.7-76.2%, the gossypol content is as low as 230-290 ppm, the residual solvent in the protein is as low as 280-340 ppm, and the product quality is high.

The invention discloses a method for rapidly predicting the net utilization rate of phosphorus in cottonseed meal by broiler chickens based on chemical component indexes and application, and belongs to the technical field of feed. The method for rapidly predicting the net utilization rate of the broiler chicken to the phosphorus in the cottonseed meal based on the chemical component indexes comprises the following steps: measuring a crude fiber content CF value, a neutral detergent fiber content NDF value and a free gossypol content FG value in the cottonseed meal to be measured; the calculated values are substituted into a mathematical model: SMA (%) = 60.99-2.697 CF (%) + 1.124 NDF (%)-0.0211 FG (mg / kg); and calculating the phosphorus net utilization rate SMA. The invention further discloses application of the method in preparation of broiler feed. According to the method, the efficient prediction model is constructed by utilizing the chemical component data, the net phosphorus utilization rate of the broiler chicken to the cottonseed meal is accurately estimated, the problems of high professional condition requirements, complicated operation, large workload, relatively long time consumption and the like in a traditional determination method are solved, and the realization of accurate preparation of broiler chicken feed is promoted.

The invention discloses a compound enzyme bacterium fermented agricultural and sideline product feed and a production process thereof, and relates to the technical field of biological fermentation, the compound enzyme bacterium fermented agricultural and sideline product feed is obtained by anaerobic fermentation of compound enzyme bacterium fermented agricultural and sideline products; the agricultural and sideline products are cotton straws, corn flour, cottonseed hulls, cottonseed meal and bran; the compound enzyme bacteria are obtained by mixing soft rot fungi, trichoderma, cellulase, clostridium, manganese peroxidase and lactic acid bacteria; according to the compound enzyme bacterium fermented agricultural and sideline product feed disclosed by the invention, the cotton byproducts are treated by adopting a microbial fermentation method, after microbial fermentation, straw lignin, hemicellulose, gossypol, crude fibers and other components, which are not suitable for being used as feed, in the cotton byproducts are degraded, and the anti-nutritional factor level in the feed is remarkably reduced; the nutritional value of the feed is greatly improved; the feed can be used as a total mixed ration, can be used for raising livestock such as cattle and sheep, greatly reduces the feeding cost, and solves the problem of low utilization rate of cotton by-products.

The invention discloses an application of a gossypol / TiO2 composite photocatalytic material in photocatalysis. The gossypol / TiO2 composite photocatalytic material is obtained by compounding gossypol and TiO2 through a sol-gel method. The gossypol / TiO2 composite photocatalytic material is obtained by compounding organic gossypol and inorganic TiO2 through a sol-gel method, the synergistic effect between organic components and inorganic components is obviously enhanced, the visible light response capability and the utilization range can be effectively improved (the visible light utilization range is expanded to about 800 nm), the photocatalytic activity is improved, and the photocatalytic effect is good. The method has very important application potential in the field of photocatalysis.

The invention provides a gossypol specific nucleic acid aptamer as well as a preparation method and application thereof. The nucleotide sequence of the aptamer is shown as SEQ ID NO. 1. A Capture-SELEX technology is adopted, a screening system is optimized, high-throughput sequencing and DNAMAN homology and secondary structure analysis are combined, the gossypol specific nucleic acid aptamer is obtained, ThT fluorescence determination and ITC verification show that the aptamer still keeps high affinity after truncation, and the gossypol specific nucleic acid aptamer can be used for preparing the gossypol specific nucleic acid aptamer. Molecular docking further defines that key basic groups such as G-26, C-27, G-28 and G-46 form a hydrogen bond-hydrophobic effect with gossypol, and a foundation is laid for construction of a subsequent gossypol rapid detection system.

The present invention relates to an extraction process and application of cottonseed peptides and cottonseed fibers. Through a series of supporting processes such as leaching with sulfurous acid aqueous solution, impact needle milling, screening with gravity and pressure curve screens, and countercurrent washing on cottonseed meal and / or cottonseed protein powder, the separation of cottonseed hulls, cottonseed fibers, and cottonseed proteins is achieved, promoting the transformation of cottonseed proteins from the bound state with cottonseed hulls and cottonseed fibers into water-soluble cottonseed proteins. After dehydration and drying, cottonseed fibers are obtained. Further, the cottonseed proteins are hydrolyzed and complex enzymatically hydrolyzed to obtain an enzymatically hydrolyzed peptide solution, which is then concentrated and spray-dried to obtain cottonseed peptides (enzymatically hydrolyzed cottonseed proteins). The extraction rate of cottonseed proteins is significantly increased, reaching 42.6 - 50.2%. The content of oligopeptides with a molecular weight below 1000 Daltons in the enzymatically hydrolyzed cottonseed proteins exceeds 82%, the content of free gossypol is below 400 mg / kg, and the removal rate is as high as over 85%. The prepared cottonseed peptides are completely water-soluble, clear and transparent, and have no bitter taste, and can be safely used in fields such as feed, food, biomaterials, cosmetics, and drugs.

The application belongs to the technical field of animal feed, and particularly relates to an egg-laying chicken feed without soybean meal, and a preparation method and application thereof. The method comprises the following steps: S1, mixing wheat flour, barley flour, cottonseed meal and glutamic acid residue, crushing the granules to obtain broken material; and S2, mixing the broken material with corn, sunflower meal, corn gluten meal, pepper residue, granular stone powder, calcium hydrogen phosphate, soybean oil, compound enzyme, sodium chloride, sodium bicarbonate, egg methionine, lysine sulfate and threonine to obtain the egg-laying chicken feed without soybean meal. The application provides comprehensive essential amino acids, especially limiting amino acids such as lysine, egg methionine and threonine, by the synergistic combination of cottonseed meal, sunflower meal, corn gluten meal and glutamic acid residue, so as to meet the nutritional requirements of egg-laying chickens. The application granulates at a temperature of 75-85 DEG C, effectively degrades free gossypol in the cottonseed meal, reduces the content of free gossypol to less than 30 mg / kg in the finished feed, and eliminates the adverse effects on the health of egg-laying chickens.

Provided are a method for improving the pigment gland character and gossypol character of a cotton seed and the use thereof. According to the method, a specific promoter of a cotton seed is used to specifically inhibit the expression and activity of the Gl2 / Gl3 protein during seed development to obtain a new cotton, the seeds of which have no pigment gland in seeds and the plants of which have a normal pigment gland, wherein the seeds thereof have no gossypol content, and the other tissues thereof have a normal gossypol content.

The present invention relates to a method for improving pigment gland traits and gossypol traits of cotton seeds and a use thereof. For the key gene Gl2 / Gl3 which determines the development of pigment glands in cotton, a cotton seed-specific promoter is utilized to specifically inhibit the expression of the gene or the activity of an encoding protein of the gene in a seed development process, so that novel cotton of which the seeds have no pigment glands and the plants have normal pigment glands can be obtained, wherein the gossypol content in the seeds of the cotton is zero, and the gossypol content in other tissues is normal.

The invention relates to the technical field of biology, in particular to an MT molecular marker HapII combination for detecting high-content solanine and application of the MT molecular marker HapII combination. MT molecular markers HapII-1 and HapII-2 which are remarkably related to the solanine content of potatoes are obtained, the molecular markers are used for detecting a potato breeding material, and the content of solanine in the potato breeding material is detected. The method can accurately and efficiently predict whether the solanine content in the potato tubers reaches 200 mg / kg or not under the condition that planting is not needed to wait for flowering and fruiting of potato plants, can efficiently assist in potato breeding selection, is a novel breeding mode which is time-saving and high in accuracy, and has extremely high economic value.

Aiming at the problems of incomplete kernel-hull separation, low oil extraction rate, standard exceeding of free gossypol and the like in the traditional process, the invention provides a whole-process collaborative optimization process, which comprises the following steps: (1) performing three-stage screening and wind power seed cleaning to ensure that the impurity content of clean cottonseeds is less than or equal to 0.5%; (2) a tooth roller type husking machine is matched with a double-layer vibrating screen and a backflow re-husking system, the husking efficiency is larger than or equal to 95%, the shell content in kernels is adjustable in 15-18%, the kernel content in shells is smaller than or equal to 0.5%, and the cotton kernel breakage rate is smaller than or equal to 3%; (3) the flaking thickness ranges from 0.3 mm to 0.4 mm; (4) the steaming and frying temperature is 100-105 DEG C, and the water content is 8-10%; (5) the oil content of the pre-pressed cake is not more than 14%; (6) the solvent leaching temperature is 40-45 DEG C, and the solvent ratio is 1: (1.2-1.5); (7) crude protein of the cottonseed meal finished product is greater than or equal to 42% and free gossypol is less than or equal to 0.02%. The PLC intelligent control system is adopted to realize parameter linkage regulation and control, the solvent recovery rate is greater than or equal to 99.5%, the unit steam consumption is less than or equal to 220kg / t cake, efficient, low-consumption and high-quality continuous production is realized, and the comprehensive utilization rate of cotton seeds and the added value of products are remarkably improved.

The invention relates to a method for improving pigment gland characters and gossypol characters of cotton seeds and application of the method. Aiming at a key gene Gl2 / Gl3 for determining the development of pigment glands in cotton, a cotton seed specific promoter is used for specifically inhibiting the expression quantity of the gene or the activity of an encoded protein of the gene in the seed development process, novel cotton with seeds free of pigment glands and normal plant pigment glands can be obtained, the gossypol content of the seeds is zero, and the cotton seed has a good application prospect. The gossypol content of other tissues is normal.