389 results about "Gossypol" patented technology

The present invention relates to naturally occurring and chemically synthesized small molecule antagonists of Bcl-2 family proteins. In particular, the present invention provides gossypol compounds (e.g., isomers, enantiomers, racemic compounds, metabolites, derivatives, pharmaceutically acceptable salts, in combination with acids or bases, and the like) and methods of using these compounds as antagonists of the anti-apoptotic effects of Bcl-2 family member proteins (e.g., Bcl-2, Bcl-XL, and the like). The present invention also provides compositions comprising gossypol compounds and optionally one or more additional therapeutic agents (e.g., anticancer / chemotherapeutic agents). The present invention also provides methods for treating diseases and pathologies (e.g., neoplastic diseases) comprising administering a composition comprising gossypol compounds and optionally one or more additional therapeutic agents (e.g., anticancer / chemotherapeutic agents) and / or techniques (e.g., radiation therapies, surgical interventions, and the like) to a subject or in vitro cells, tissues, and organs.

The present invention relates to the field of feed and oil processing technology, in particular, it relates to an one-step method for producing dephenolized cotton protein. It is characterized by that said invention fully utilizes the characteristics of 6# solvent and methyl alcohol mixed solvent, and utilizes the mutual incompatibility of polar solvent and non-polar solvent and simultaneously uses two solvents in one equipment so as to attain the goal of simultaneously extracting gossypol and oil, and can recover 6# solvent, methyl alcohol and oil.

The present invention relates to naturally occurring and chemically synthesized small molecule antagonists of Bcl-2 family proteins. In particular, the present invention provides gossypol compounds (e.g., isomers, enantiomers, racemic compounds, metabolites, derivatives, pharmaceutically acceptable salts, in combination with acids or bases, and the like) and methods of using these compounds as antagonists of the anti-apoptotic effects of Bcl-2 family member proteins (e.g., Bcl-2, Bcl-XL, and the like). The present invention also provides compositions comprising gossypol compounds and optionally one or more additional therapeutic agents (e.g., anticancer / chemotherapeutic agents). The present invention also provides methods for treating diseases and pathologies (e.g., neoplastic diseases) comprising administering a composition comprising gossypol compounds and optionally one or more additional therapeutic agents (e.g., anticancer / chemotherapeutic agents) and / or techniques (e.g., radiation therapies, surgical interventions, and the like) to a subject or in vitro cells, tissues, and organs.

This invention relates to pulsatile dose administration of gossypol or pharmaceutical compositions thereof for treating diseases, disorders and conditions responsive to gossypol, inhibiting the activity of anti-apoptotic Bcl-2 family proteins, inducing apoptosis in cells and increasing the sensitivity of cells to inducers of apoptosis.

The invention discloses a method for producing fermented feed containing probiotics for pigs by using microbial fermentation, comprising the following steps: (1) cultivating aerobic fungi for producing strains; (2) cultivating bacillus producing lactic acid; and (3) solid-state cultivating: 1) thoroughly mixing soya bean dregs, cotton dregs, vinegar residue with wheat bran and / or rice bran and adding water to prepare a mixture; 2) inoculating the producing strains prepared in the step (1) and the step (2) into the mixture; and 3) cultivating by stacking or bag stacking to obtain an antibiotics-free fermented feed. The method of the invention is simple and convenient. The products have stable properties and are easy for large-scale producing. Using the method to prepare the feed for the pigs can effectively reduce anti nutritional factors in the feed and the method plays a role in the gossypol reduction of the cotton dregs, thereby decreasing the input amount of inorganic phosphorus to the feed, increasing the utilization of organic phosphorus in the raw material of the feed, accelerating the absorption of nutrients in the feed for pigs and reducing the environment pollution due to the feed.

The invention discloses a method for removing cotton rapeseed meal toxicant by fermentation method and enhancing nutrient value thereof, a feeding fermented cotton rapeseed meal protein feedstock and applications thereof. The method comprises the steps that: cottonseed meal and rapeseed meal are mixed, the mixture is first added with feedstock water and complex enzyme preparation, and then inoculated with microorganism strain capable of producing protease bacteria or / and lactic acid bacteria as well as decomposing glucosinolate or / and gossypol, then fermentation is performed to obtain treated cotton rapeseed meal so as to obtain nontoxic, highly-nutrient feeding fermented cotton rapeseed meal protein feedstock, and the feeding fermented cotton rapeseed meal protein feedstock is used as protein feedstock component to form cotton rapeseed meal animal feed according to 15% of the addition proportion. Using modern biological technology, the method solves the problems of low utilization rate of cotton rapeseed meal, small amount of addition in the feed, the presence of toxicant and the like, and eases the strain on feed protein in our country.

The invention utilizes a microbial fermentation technology to improve the forage nutritional value of the cottonseed meal, so as to improve the forage quality of the cottonseed meal, increase the addition proportion in the forage and realize the promotion of the protein nutritional value and the feeding value of the cottonseed meal, aiming at the disadvantages that cottonseed meal is rich in antinutritional factors of free gossypol, cellulose, oligosaccharide, phytic acid and the like, the protein quality is relatively poor and the content of essential amino acids of methionine and the like is low and unbalanced, so that the large-scale forage of cottonseed meal is restricted. The method for improving forage nutritional value of cottonseed meal through the microbial fermentation adopts the technology of 'composite enzymatic hydrolysis and multi-strain three-step solid-state fermentation', so as to prepare the fermented cottonseed meal product that is high in the free gossypol, cellulose degradation degree and the protein content; the cottonseed meal product is rich in nutritional ingredients of probiotics, vitamins, enzyme, amino acid, short peptide, high-grade protein and the like; the method for improving the forage nutritional value of cottonseed meal through the microbial fermentation has the advantages of prompting growth and development, improving the utilization ratio of the forage, enhancing immunization, improving intestinal micro ecology, preventing diseases and the like. The method for improving forage nutritional value of cottonseed meal through the microbial fermentation adopts the solid-state fermentation; the manufacturing technology is simple; the energy consumption is low; zero environmental pollution is generated; and the investment is small. Therefore, mass production is easy.

The invention relates to the technical field of wood processing and discloses a green mould-proof insecticide for wood materials. The green mould-proof insecticide is prepared from the following raw materials: cotinus coggygria leaves, Chinese pine leaves, mountain peach bark, catalpa leaves, melia azedarach leaves, paper mulberry leaves, periploca bark, corylus mandshurica, physocarpus monogynus bark, fraxinus malacophylla, clematis finetiana, edgeworthia chrysantha bark, Chinese thorowax root, butea monosperma seeds, centipedegrass, white granulated sugar, gossypol, lactic acid bacteria, wine yeasts and compound enzymes. The raw materials are rich and cheap, are simple to prepare, are green and healthy, are free of any toxic reagent, are friendly to the environment, and are harmless to the human body. Steamer water is reserved and used for extracting and can prevent mould, kill insects, increase flexibility and facilitate processing; and at the same time, water usage can be saved, the environment can be protected, and the cost can be saved by 10.2%. Through the double fermentation of the lactic acid bacteria and the wine yeasts, plant cell structures are destroyed, the extracting of effective components is promoted, small-molecular antibacterial components are increased, the mould-proof and insecticidal effect is enhanced, the antibacterial rate reaches 81.7%, and the insecticidal rate reaches 78.3%.

The invention discloses liquid preparation with gossypol or other analogs. The liquid preparation contains (a) 0.01to 10 weight percent of gossypol or other analogs; (b)12 to 20 weight percent of polyoxypropylene polymer, alginic acid or chitosan; (c) 0.5 to 10 weight percent of poly (dimethylsiloxane), poly (dimethylsiloxalkanol) or dimethylsiloxalkyl ether polymer; and (d) 1.0 to 2.5 weight percent of acidic buffering agent. The invention also discloses a preparation method and usage of the liquid preparation.

The invention relates to application of a gossypol derivative to preparing an anti-tumor medicament and discloses a novel gossypol derivative, use of the derivative for preparing a medicinal composition for preventing and treat tumors, and the medicinal composition comprising the gossypol derivative and a preparation method thereof.

The invention discloses a comprehensive method for preparation of raffinose and gossypol from cottonseed meal. The method comprises the technological steps of: (1) extracting oil-extracted cottonseed meal with a polar solvent so as to obtain an extracted solution; (2) filtering the extracted solution, and removing an organic solvent in vacuum so as to obtain an aqueous phase and a solid phase; (3) subjecting the aqueous phase to filtration, flocculation, decoloration and desalination, decoloration, as well as desolventizing, thus obtaining raffinose; (4) first adding enzymes into the solid phase to carry out hydrolysis, then using an acid to adjust the solution to acidic, and performing precipitation and separation, thus obtaining a solid; (5) adding ether, halohydrocarbon, ester and / or low level ketone solvents to dissolve gossypol, and then filtering out impurities to obtain a gossypol-containing filtrate; and (6) subjecting the gossypol-containing filtrate to crystallization and crystal growing under nitrogen protection, then conducting filtration and drying, thus obtaining acicular crystals. The method can realize combined industrial production of dephenolized cottonseed protein, raffinose and gossypol, is suitable for large-scale production, and realizes comprehensive processing as well as maximum valuation of cottonseeds.

The invention relates to a biological fermentation process for improving the protein content of cottonseed meal and detoxicating the cottonseed meal, which is characterized in that: the byproduct of cottonseed subjected to oil extraction, namely the cottonseed meal is taken as a raw material; the cottonseed meal is processed by adopting microbial fermentation technology; a cottonseed meal mixed fermentation process is adopted; strains are combined, cell walls of a plant are broken and free gossypol and anti-nutritional factors contained in the cottonseed meal, such as cyclopropene fatty acid, phytic acid, tannin, non-starch polysaccharide and non-glycan are modified, transferred, recombinated, purified and detoxicated; and proteins in the cottonseed meal which have large molecular weight and are difficult to absorb by animals are degraded into small active peptides by a biological enzymolysis method, and probiotics, digestive enzymes, lactic acid and various bioactive factors in the product are kept. The biological fermentation process can greatly improve the protein content of the cottonseed meal and effectively achieve the detoxicating effect.

The invention provides a fermented feed and a production process thereof, wherein the fermented feed forms composite fermentation strains by three groups of lactic acid bacteria, yeast and bacillus which have more than ten bacterial strains, the composite fermentation strains are mixed with molasses according to the ratio of 1:1 and are mixed with fermentation zymolyte (cottonseed meal is mixed with rapeseed meal with the grain size of 1.0 to 2.0mm according to the ratio of 90:10-60:40) to form fermentation material according to the ratio of 0.1 percent, after the moisture content of the material is adjusted to 40-60 percent, fermentation of 30 plus or minus 2 DEG C is carried out for 48 hours, and expansion drying under 80 DEG C is carried out after completing the fermentation. Compared with non fermentation, a fermentation miscellaneous meal product produced by the production process reduces the gossypol, isothiocyanate and oxazolidinethione contents by more than 80 percent, improves the crude protein content by more than 5 percent, and has the lactate content larger than 2.5 percent and pH value about 4.5. The product can substitute soya bean meal of 30 to 50 percent in small, medium and large pig feed, the feed cost is lowered by 20 to 50 yuan / ton, feed intake is improved by about 10 percent, the feed meal has close phase comparison, daily gain is improved by about 4 percent, the morbidity is greatly reduced, and the ammonia odour of a colony house is obviously reduced.

The invention relates to a method for extracting raffinose from cottonseed meal, including following steps: removing the cottonseed rind to get cottonseed kernel, softing the embryo of the cottonseed kernel, dipping the cottonseed oil with solvent-extracted oil, getting cotton dregs; adding 40-100wt.% of carbinol in the cotton dregs, dipping for 20-60 minutes at 0-70 DEG C, after removing the carbinol, getting coarse raffinose; dissolving the coarse raffinose by water or rare acid, controlling temperature at 0-100 DEG C, pH=2-7, filtering, decoloring, concentrating, crystallize, drying, getting extractive raffinose. The invention re-exacts the raffinose after degreasing, ensures to attain the raffinose as well as perfect conttonseed oil, and reduces the dissociative gossypol content in the cotton dregs and improves the cotton dreg quality.

This invention relates to pulsatile dose administration of gossypol, a gossypol-related compound or pharmaceutical compositions thereof for treating diseases, disorders and conditions responsive to gossypol or gossypol-related compound, inhibiting the activity of anti-apoptotic Bcl-2 family proteins, inducing apoptosis in cells and increasing the sensitivity of cells to inducers of apoptosis.

This invention relates to pulsatile dose administration of gossypol or pharmaceutical compositions thereof for treating diseases, disorders and conditions responsive to gossypol, inhibiting the activity of anti-apoptotic Bcl-2 family proteins, inducing apoptosis in cells and increasing the sensitivity of cells to inducers of apoptosis.

The invention relates to gossypol derivatives and preparation method thereof and application of the gossypol derivatives in plant virus prevention and control, insecticidal action, bactericidal action and anti-cancer action, wherein the significance of all groups in a formula is shown in the description. The gossypol derivatives show the excellent anti-plant virus activity, bactericidal activity, insecticidal activity and anti-cancer activity.

The invention discloses a microbial leavening agent of cottonseed meal as well as a preparation method thereof. The preparation method comprises the following steps: by taking brewer's yeast and lactobacillus plantarum as strains, carrying out enlarging cultivation by means of liquid fermentation; adding a protective agent to prepare the microbial leavening agent, wherein the total viable organisms of the microbial leavening agent is 2*10<9>cfu / g. The microbial leavening agent prepared by the method disclosed by the invention can remarkably degrade free gossypol in cottonseed meal and effectively inhibit growth of infectious microbes in the fermentation process, so that the acidity is reduced and the palatability is improved. In a detoxification fermentation process, a par of plant proteins is further converted into mycoproteins to increase B vitamins and active enzymes so as to have the effect of increasing the protein sources and bio-additives, thereby greatly improving the use value of detoxification fermentation of cottonseed meal. The preparation method is simple in strains and fermentation process, low in cost, good in fermentation effect, low in cottonseed meal fermentation cost and strong in market competitiveness.

The present invention discloses a method for decoloring raffinose extract by adopting fixed bed type adsorptive separation. In the method, after raffinose alcohol extract is condensed and alcohol is removed, aqueous solution, the solid content of which is 10 percent, is prepared; pH is regulated to 4 to 6; the prepared solution passes through a fixed bed absorption column containing particulate active carbon, non-polar absorption resin or weak-polar absorption resin in order to remove pigments such as gossypol; and finally, 60 percent to 75 percent ethanol is used to regenerate the absorption column. In the method, the decoloring rate of the extract is more than 90 percent, and the yield of raffinose is higher than 95 percent. The method has the advantages of simple process flow, convenient adsorbent regeneration, low production cost, easy industrialized production, and the like.

The invention belongs to the technical field of processing of oil-bearing crops, and particularly relates to a technique for simultaneously extracting lipid and gossypol from cotton seed. The invention provides a clean production technique for processing cotton seed, which has the advantages of great convenience, high efficiency, low cost and is easy to operate and control; and the existing cotton seed leaching plant can be slightly reconstructed to implement industrialized large-scale production of simultaneously extracting cotton oil and gossypol from cotton seed as well as coproduction of detoxified cotton seed protein. Compared with the prior art, by utilizing mixed solvent technology and ethanol secondary leaching technology, the technique can reduce the consumption of the No.6 solvent by 30-60%, and greatly reduce the requirement and dependence of the grease processing industry for the petroleum product; the technique can obtain high-quality gossypol and phospholipid on the premise of not generating wastewater, thereby eliminating a large pollution source of cotton seed processing industry; and meanwhile, the technique can enhance the yield of cotton oil, gossypol and detoxified cotton seed protein by 3-5%, and greatly lower the production cost, thereby having wide application prospects in the field of cotton seed processing.

A method of preparing gossypol and its derivative polydopamine nano-carrier by the polymerization process includes: dissolving gossypol or its derivatives in an organic solvent, dropwise adding in a weakly alkaline buffer the organic solvent in which the gossypol or its derivatives is dissolved, adding dopamine into the buffer, and allowing reacting at room temperature for 4-72 hours. The weakly alkaline buffer is used to dialyze the polydopamine nano-carrier encapsulating the gossypol and its derivative, with un-covered drugs removed; the method is simple and quick, drug covering rate and drug carrying capacity are higher, especially the need for a surfactant is avoided, and thus the method is a simple, easy novel process of encapsulating the gossypol and its derivatives with dopamine.

The invention relates to a method for processing animal feed, and discloses a method for producing the animal feed based on cottonseed meal physical, chemical and microbial detoxification technology, comprising hot treatment, chemical treatment and microbial treatment, wherein the hot treatment is high temperature steam fumigating; the chemical treatment is composite mineral impregnation; and the microbial treatment is solid state fermentation of saccharomyces cerevisiae and aspergillus oryzae. By compound detoxification, the method greatly reduces the content of free gossypol in the cottonseed meal and leads the content of the free gossypol to reach the feed standard; simultaneously, as the microbial detoxification method is introduced, the time for hot treatment can be effectively shortened, the addition of chemical drug is reduced, and the nutritional value of the cottonseed meal can be improved.

The invention provides biocompatible micelles loaded with one or more active agents. The micelles can encapsulate anticancer drugs such as gossypol, and combinations of drugs, such as gossypol and paclitaxel, gossypol and 17-AAG, gossypol and cyclopamine, gossypol, paclitaxel, and 17-AAG, and gossypol, paclitaxel, and cyclopamine. The micelle compositions provide effective solubilization of difficult to solubilize drug combinations without the need for additional surfactants that can be toxic to patients. Thus, the invention provides stable and biocompatible drug formulations that improve bioavailability without causing toxicity.

The present invention provides a biomarker for selecting a patient for treatment with gossypol, wherein the biomarker comprises an elevated expression level of c-Myc, Mcl-1, or combination thereof, relative to the normal expression level of c-Myc, Mcl-1, or combination thereof. The present invention also provides methods for targeting patients for treatment with gossypol, wherein the patient has a disease, condition, or disorder that overexpresses c-Myc, Mcl-1, or combination thereof. The present invention also provides methods for treating or ameliorating a disease, condition, or disorder in a patient comprising determining the expression level of c-Myc, Mcl-1, or combination thereof in the patient and administering gossypol to the patient. In certain embodiments of the invention, the disease is cancer, and the cancer cells show elevated expression levels of c-Myc compared to non-cancerous cells. The invention also provides methods for overcoming Mcl-1-mediated chemoresistance comprising administering gossypol to a patient in need thereof.

The invention discloses a biological detoxification method of cottonseed meal, comprising: adding ferrous sulfate heptahydrate, 40,000-60,000 U / g of neutral xylanase, microorganism strains and the like into smashed cottonseed meal; after being evenly stirred, jointly acting for 4-5 days by microorganism and neutral xylanase at the temperature of 45-55 DEG C; and drying and smashing to obtain the product. The free gossypol content of the obtained product is lowered than the safety index specified by feed, the nutritional value thereof is higher than that of unleavened cottonseed meal, product protein content reaches 45-50%, free amino acid content is above 15%, and the cottonseed meal can be directly used as protein feed. The invention better solves the problems of incomplete detoxification of cottonseed meal, protein denaturation and secondary feed pollution due to adopting the chemical detoxification method in the prior art, and provides a non-toxic cottonseed meal production method which has favorable detoxification effect and low technical energy consumption and can reach feed standard requirements.

The invention discloses a method for fermenting and decomposing gossypol in cottonseed meal by composite strains. Corynebacterium, Torula utilis Henneb, Bacillus subtilis and lactic acid bacteria are used for fermenting and culturing to prepare composite strain fermentation broth; then, the composite strain fermentation broth and a cottonseed meal raw material composed of cottonseed meal, corn steep liquor, molasses, ammonium sulphate, urea and a 10% sodium hydroxide aqueous solution raw material are evenly mixed; and then a solid is fermented, dried, smashed and packaged by a sealed bag to finish decomposing the gossypol in the cottonseed meal. The protein solubility of the fermented cottonseed meal is improved by above 50%, the feed cost is lowered, the breeding benefit is increased, and the cottonseed meal has a wide use range.

A method for extracting raffinose from cottonseed meal is characterized in including the following steps: (1) extracting oil from the cottonseed meal, and extracting in 85-95 wt% methanol at the room temperature to 65 DEG C for 30-100 min. to obtain methanol extractive solution containing phenol and raffinose; (2) distilling at 80-100 DEG C for removing methanol and gossypol, and concentrating to obtain the raffinose crude product; (3) dissolving into water and / or diluted acid at the room temperature to 80 DEG C to adjust the pH value to 2-7; and (4) filtering, decoloring, concentrating, crystallizing, and drying to obtain refined raffinose. The method has the advantages of complete removal of methanol and gossypol from raffinose, high yield of raffinose, remarkable economic benefit, convenient operation, and suitability for large-scale industrialized production.

The invention discloses a preparation method of cottonseed protein, which comprises the steps that (1) cottonseed meal is dephenolized; and (2) the cottonseed protein is extracted from the dephenolized cottonseed meal, wherein dephenolization of the cottonseed meal is to leach the cottonseed meal with a mixed solvent of alcohol and n-hexane, and a volume ratio of alcohol to n-hexane is (1-5):5. The dephenolized cottonseed meal is leached by a mixed aqueous solution of sodium hydroxide and sodium sulfite, and the cottonseed protein is obtained by acid precipitation of a leaching solution. With the adoption of the method, a removed rate of gossypol can reach above 80%; free gossypol (FG) is less than or equal to 400mg / kg; a yield of the cottonseed protein reaches above 60%; and the purity is greater than 90%.

The invention discloses a method for degrading gossypol in cottonseed meal by applying oxidase. The method comprises the following steps of: spraying an oxidase aqueous solution and hydrogen peroxide with concentration of 1 / 10000 to the surface of cottonseed meal, wherein the mass ratio of the oxidase aqueous solution to the cottonseed meal is (1:9)-(4:1); and keeping the oxidase aqueous solution and the cottonseed meal for 10-30 hours at 20 DEG C-40 DEG C, and taking out the cottonseed meal. According to the method for degrading gossypol in cottonseed meal by applying oxidase disclosed by the invention, the oxidase can be used for degrading bound gossypol and free gossypol in the cottonseed meal, and the substances generated by degrading are pyrocatechin, i.e., catechol and a derivative thereof. The catechol and the derivative thereof have an antibacterial effect and an anti-oxidation effect, so that the adding amount of the antibiotics and antioxidant in the cottonseed meal feed is reduced, and therefore, the method not only can be used for detoxifying, but also can be used for lowering the amount of the antibiotics and the antioxidant, and is simple and economical.

The invention provides a production process of fermented cottonseed meal. The process comprises the following steps: breeding lactobacilli, bifidobacteria, bacillus subtilis and candida utilis by adopting a self-made culture medium at the same time, mixing obtained strain liquid, acid protease, cellulase, aspergillus oryzae and yeast enzyme with cottonseed meal, performing fermentation production, drying, grinding and packaging. According to the fermented cottonseed meal produced by adopting the process, free gossypol in the cottonseed meal can be effectively degraded, the palatability of the cottonseed meal can be improved, macromolecular proteins are decomposed into micromolecular polypeptides or amino acids convenient to absorb by animals, crude proteins and crude fibers are degraded, a large quantity of active probiotics are secreted and synthesized, and the nutritional value of the cottonseed meal as a feed is improved.