517 results about "Zearalenone" patented technology

The invention discloses a mycotoxin detoxification agent for feed and a preparation method and a feed additive thereof, and belongs to the field of feed additives. The mycotoxin detoxification agent can be used for detoxification of feed raw materials and compound feed of mycotoxin polluted corn, grains and the like. The mycotoxin detoxification agent consists of modified montmorillonite, yeast cell wall, chitosan and the like; and the preparation method has simple process. The novel mycotoxin detoxification agent for feed can effectively remove pollution of multiple mycotoxins such as aflatoxin, zearalenone, T2 toxin, vomitoxin and the like in the feed raw materials and the feed of the corn, the grains and the like, and prevent various diseases of livestock and poultry because of eating the mycotoxin polluted feed. The mycotoxin detoxification agent has simple preparation process, stable and controllable quality, convenience in use and low price, meanwhile has the advantages of supplementing nutrients, improving the disease resistance of cultured animal bodies and avoiding adsorbing nutrient elements in the feed, and guarantees the health and safety of the cultured animals.

The invention relates to the technical field of agricultural biologics and in particular relates to Bacillus velezensis ANSB01E CGMCC No. 17328 and application thereof. The Bacillus velezensis provided by the invention is capable of simultaneously and efficiently degrading zearalenone and aflatoxin, and degradation rates upon g zearalenone and aflatoxin are both up to 90% or greater 48 hours laterafter reactions. The Bacillus velezensis provided by the invention is high in toxin degradation efficiency, good in specificity, mild in action effect, free of damage to nutrient components in feedsand applicable to biological detoxification of mycotoxins in cereals, feeds and foods.

The invention discloses a food-grade aspergillus niger strain FS-Z1 which is separated out from sauce grains and capable of preventing and treating zearalenone toxin. The collection number of the food-grade aspergillus niger strain FS-Z1 is CCTCC NO: M 2013703. The strain has excellent degradation effect on the zearalenone and the degradation rate reaches 89.56%. Besides, animal experiments in rats verify that the zearalenone in the corn steep liquor degraded by using an aspergillus niger fermentation liquor has no toxicity.

The invention discloses a zearalenone toxin gradation enzyme and a coding gene and application thereof. The enzyme is derived from Gliocladiumroseum and is protein in (a) or (b): (a) protein consisting of an amino acid residue sequence of SEQIDNO: 1 in a sequence table; and (b) protein which is derived from the SEQIDNO: 1, has a zearalenone degradation activity and is formed through carrying out substitution and / or deletion and / or addition of one or a plurality of amino acid residues on the amino acid residue sequence of SEQIDNO: 1 in the sequence table. The zearalenone toxin degradation enzyme has highly-efficient degradation effect on zearalenone (ZEN).

A feed nano additive for adsorbing fungal toxin in feed is prepared through proportionally mixing montmorillonite, sodium chloride and water, stirring, water washing, preparing pulp again; adding glucomannan while high-speed stirring, reaction, ageing, water washing, dewatering; drying filter residue and breaking. Its advantages are broad-spectrum adsorption of different toxins, high adsorptive power, no influence of pH value, low residual and low dosage (0.02-0.2%).

The invention discloses a bacillus amyloliquefaciens and an application thereof in degradation of zearalenone and provides a bacillus amyloliquefaciens NS2 which is assigned the accession number CGMCC No.8726. An experiment in the invention proves that the amyloliquefaciens NS2 is disclosed in the invention. The zearalenone can be degraded efficiently when a fermentation liquor of the amyloliquefaciens NS2 is added to the zearalenone with dissolving and mixing uniformly. The amyloliquefaciens NS2, used as a biological material for degrading the zearalenone, has a good application prospects in exploitation of not only novel biodegradation inoculants but also novel biodegradation sterile preparations.

The invention relates to a method for detecting the content of mycotoxins in wheat. The mycotoxins in the wheat are detected by utilizing high performance liquid chromatography-tandem mass spectrometry, and the mycotoxins related to the detection are deoxynivalenol (DON), 3-acetyl (Ac)-DON, 15-Ac-DON, zearalenone (ZEN) and T-2 respectively. The detection method specifically comprises the following steps of: crushing wheat samples by using a crusher, and obtaining coarse extract by using an extracting solution; obtaining a purified toxin extracting solution by using an amino column; preparing mixed toxin standard liquid with different content; detecting each mycotoxin in the toxin extracting solution by adopting the high performance liquid chromatography-tandem mass spectrometry; and obtaining a regression equation of mycotoxin content relative to a peak area according to detection results of the mixed toxin standard liquid, and calculating the content of the DON, the 3-Ac-DON, the 15-Ac-DON, the ZEN and the T-2 in the wheat sample.

The invention relates to a bacterial strain for degrading zearalenone toxin, which is characterized in that: the preserving number of the bacterial strain is CCTCC NO: M2010053; and the Genbank accession number of the bacterial strain 16S rDNA is HM372880. When in application, the bacterial strain is prepared into liquid bactericide or solid bactericide which can degrade the zearalenone toxin for degrading the zearalenone toxin in grains or fodders. The bacterial strain has the advantages that: the process of using the bacterial strain to degrade the zearalenone toxin is free of secondary pollution, thereby improving the quality of agricultural products and ensuring the edible safety of people and livestock; and the bacterial strain is used for producing various preparations which are used for degrading the zearalenone toxin the bacterial strain, which is not only low in production and using cost, but also safe in use.

The invention discloses a method for biodegrading mycotoxin in feed by using a mycotoxin degrading enzyme, and belongs to the field of feed resources. The method comprises the following steps: directly mixing degrading complex enzyme of aflatoxin, zearalenone and deoxynivalenol with moldy feed raw materials, concentrated feed or complete feed; and detoxifying the feed polluted by the mycotoxin to ensure that the degradation rate of the mycotoxin reaches over 80 percent. The method can effectively remove the mycotoxin from the feed, improves animal health and animal production performance, and can effectively solve the problem of feed resource shortage in China.

The invention discloses Bacillus subtilis for effectively degrading zearalenone and application thereof. The preservation serial number of the Bacillus subtilis ASAGF141 is CGMCC No.9463. The invention further provides bactericide containing preserved strains and a preparation method of the bactericide; besides, the invention further provides application of the strains or the bactericide to degradation of the zearalenone. According to the Bacillus subtilis for effectively degrading zearalenone and application thereof, the trains can completely degrade the 20 microgram / ml zearalenone within a short time, and the degradation rate is 10%.

The invention relates to a ZEN (zearalenone) degrading enzyme gene and a high-yield strain. The ZEN degrading enzyme gene comprises DNA molecules of (a), (b) or (c), wherein (a) has DNA molecules of a nucleotide sequence shown in SEQ ID NO.1; (b) hybridizes with the nucleotide sequence in (a) under a strict condition and encodes DNA molecules of protein with ZEN degrading enzyme activity; (c) has DNA molecules of a nucleotide sequence with 90% or higher of homology with the nucleotide sequence in (a) or (b). The invention establishes the high-yield strain for producing ZEN degrading enzymes through the sequences and further provides a high-density fermentation method. ZEN degrading enzymes can be subjected to efficient secretion expression through the high-yield strain with the high-density fermentation method, and ZEN can be quickly and efficiently degraded during fermentation.

A detection method includes preparing single cloning antibody by steps of antigen synthesis, mouse immune, cell fusion, hybrid tumor selection and its cell line collection, extracting ZEN in corn sample by methanol / aqueous solution; filtering and deluting it; flowing liquid sample through affinity column and it by methanol; and using fluoresclence spectrophotometer to measure out content of zearlenone in set sample solution.

The invention discloses a preparation method of an antibacterial attapulgite zearalenone adsorbent. The preparation method comprises the following steps: performing acidification treatment on attapulgite with a compound acidifier, screening to remove quartz and carbonate impurities, adding an ionic liquid surfactant and performing reaction for 4-12 hour at the temperature of 40-90 DEG C; and separating, washing, drying and crushing the product to obtain the antibacterial attapulgite zearalenone adsorbent. The prepared attapulgite zearalenone adsorbent is not only capable of adsorbing and removing mycotoxin out of feed and also has an antibacterial function and the usage of antibiotics in the feed can be reduced.

The invention provides a toxin adsorption antidote capable of eliminating harm done by mycotoxins to livestock. The toxin adsorption antidote is prepared from 45-55% of modified attapulgite, 20-28% of modified montmorillonite, 5-10% of lignocelluloses, 5-15% of yeast cell walls, 1-2% of multi-vitamin micro-mineralization nutritious supplementary and 3-5% of functional extract superpacket. The toxin adsorption antidote can adsorb common mycotoxins, including aflatoxin, zearalenone, vomitoxin, ochratoxin and fumonisins, in feed so as to reduce the harm done by mycotoxins to animals; meanwhile, adsorption detoxication function is realized through nutrition enhancement and immunity improvement of livestock, and livestock breeding effect is improved.

This invention relates to one agent case and its test method for corn zeranol, which is based on label immune reaction, wherein, the micro hole board comprises ZEN-BSA added with ZEN antibody; the free ZEN and micro board ZEN-BSA competes for ZEN antibody and the free ZEN antibody is removed with labeled sheep antipest antibody; the labeled immune reaction labeled antibody is removed; after adding strengthen liquid it uses time resolution fluorescence device to test the intensity cps with its concentration reverse to ZEN concentration to determine the ZEN content by comparing standard curve and sample.

The invention discloses pseudomonas aeruginosa and an application thereof in degrading zearalenone. The preservation number of the pseudomonas aeruginosa is CGMCC No.8727. As a biologic material for degrading zearalenone, the pseudomonas aeruginosa is good in application prospect in no matter developing a novel biologic degrading bacterium agent or a biologic degrading bacterium-free preparation.

The invention belongs to the technical field of agriculture animal husbandry and food antitoxin and detoxication, and relates to mycotoxin adsorbent and a preparation method thereof. The mycotoxin adsorbent is composed of, by weight, 85-90% of montmorillonite, 5-7% of yeast cell walls, 1-3% of chitin, 1-2% of saccharomyces boulardii and 3-5% of natural plant extract in a mixing mode. The natural plant extract comprises tea tree oil, hesperidin, eugenol, citral, cinnamaldehyde and baicalein which are proportionally mixed. The mycotoxin adsorbent can be applied to fodder, aflatoxin B1 in the mycotoxin can be absorbed, other mold toxins such as zearalenone, ochratoxin, deoxynivalenol and fumitremorgin can also be effectively absorbed, the nutrient absorption rate of the fodder is low, meanwhile, breeding and balancing of probiotics in intestinal canals can be promoted, and the immunocompetence of animals is enhanced.

A microorganism for the biological inactivation or detoxification of mycotoxins, in particular ochratoxins, which is selected from bacteria and / or yeasts, which cleaves the phenylalanine group of the mycotoxins, in particular ochratoxins, as well as a method for biologically inactivating or detoxifying mycotoxins, in particular ochratoxins, in food products and animal feeds by the aid of a microorganism, and the use of the microorganism(s).

The invention relates to the field of clay modification, in particular to phospholipid modified clay and a preparation method and application thereof. A modifying agent for the modified clay comprises phospholipid, and the modified clay is applied to a mycotoxin adsorbent. The phospholipid and the clay are innovatively compounded to prepare the mycotoxin adsorbent, and since phospholipid which belongs to feed additive ingredients is allowed to be added into feed, the safety problem possibly caused by traditional compounding of quaternary ammonium salt and the clay is solved. The compound mycotoxin adsorbent is excellent in adsorption effect on various toxins including aflatoxin, zearalenone and the like.

The invention provides a hybridoma cell strain 2D3, a monoclonal antibody to zearalenone secreted by the hybridoma cell strain 2D3 and application of the monoclonal antibody. The hybridoma cell strain 2D3 is preserved in China Center for Type Culture Collection with an accession number of CCTCC No. C201328 and can be used for preparation of a high-titer monoclonal antibody to zearalenone. According to detection results of enzyme linked immunosorbent assay (ELISA), the titer of the monoclonal antibody to zearalenone prepared through purification of mouse ascites can reach 1.5 * 10<5>. The monoclonal antibody to zearalenone has high sensitivity, half maximal inhibitory concentration IC50 of 20 pg / mL to zearalenone and cross reactivity of 4.9%, 3.3% and 3.2% with beta-zearalanel, alpha-zearalanel and beta-zeranol, respectively. The monoclonal antibody to zearalenone can be used for determination of the content of zearalenone.

The invention discloses bacillus amyloliquefaciens capable of degrading ZEN (zearalenone) efficiently and an application of the bacillus amyloliquefaciens. The collecting number of the bacillus amyloliquefaciens ASAGF142 is CGMCC (China General Microbiological Culture Collection Center) No. 9464. The invention further provides an inoculant comprising the collected strain and a preparation method of the inoculant. Besides, the invention provides the application of the strain or the inoculant in degradation of ZEN. The strain can degrade 20 mu g / ml of ZEN completely in short time, and the degradation rate is 100%.

The invention relates to an ELISA kit for detecting zearalenone, the detection is rapid, sensitive, accurate, quantitative, simple in operation, low in requirements on sample purity and strong in specificity, thereby being particularly applicable to the detection of large quantities of samples; and the invention also provides a preparation of the kit and a detection method. The kit comprises washing liquid, color developing liquid A, color developing liquid B and stop solution, and the kit is characterized in that: the kit also comprises a coated plate, a zearalenone standard product, a zearalenone monoclonal antibody freeze-dried product and an enzyme-labeled goat anti-mouse antibody free-dried product; when in detection, the coated plate is taken, 50mu1-100mu1 of the ZEN standard product or a well processed sample is added into the respective micropores, 50mul-100mul of the anti-ZEN antibody is added, the incubation is carried out at 35 DEG C-45 DEG C for about 0.5 hour-1 hour, the washing liquid is used for washing for 3 times-5 times, 50mu1-100mu1 of the horseradish peroxidase (HRP)-goat anti-mouse antibody is added, the incubation is carried out at about 35 DEG C-45 DEG C for about 0.5 hour-1 hour, the washing liquid is used for washing for 3 times-5 times, 50mu1-100mu1 of the color developing liquid A and 50mu1-100mu1 of the color developing liquid B are added, the mixture is placed still in the dark for 10 minutes-20 minutes, then the stop solution is added, the absorbance value is measured at 450nm, and the ZEN content in the sample is calculated from a standard curve.

The present invention relates to a method for detoxification of feed products contaminated by the mycotoxin zearalenone.

The invention belongs to the technical field of food safety detection, and provides a high-efficient liquid chromatogram method for simultaneously detecting aflatoxin B1, ochratoxin A, zearalenone and citrinin in grains. Universal type extracting solution is extracted, detection is realized by a high-efficeint liquid chromatogram and fluorescence detector method, and quantification is realized by an external standard method. Detection limits of the method respectively include 1.08 micro-grams / kg of aflatoxin B1, 3 micro-grams / kg of ochratoxin A, 35.85 micro-grams / kg of zearalenone and 6.52 micro-grams / kg of citrinin. The method is simple, convenient and fast, is low in environmental pollution and detection cost, and is applicable to simultaneously and quantitatively detect four types of mycotoxin in the grains.

The invention relates to zearalenone degrading enzyme and a mutant as well as a coding gene and application thereof. The degrading enzyme and the mutant have amino acid sequences shown as SEQ ID NO.1and SEQ ID NO.3, or the degrading enzyme and the mutant are conservative mutants obtained by missing, replacing, inserting or / and adding one or several amino acid on the basis of amino acid sequencesshown as SEQ ID NO.1 and SEQ ID NO.3. The zearalenone degrading enzyme and the mutant have the advantages of high enzyme activity, good temperature and pH tolerance and the like, and can be widely applied to enzymolysis of zearalenone and several derivatives of the enzymolysis; the substrate range is wide.

The invention relates to a method for degrading zearalenone, which is characterized in that: a fermentation product of aspergillus niger is used for degrading zearalenone in musty grain, wherein the fermentation product of the aspergillus niger is the dry powder of fermentation liquor. In the invention, because the fermentation product of aspergillus niger is used for degrading zearalenone, zearalenone in the musty grain can be effectively degraded, thereby restoring the application value of the musty grain. Meanwhile, by measuring the effect of the fermentation product of aspergillus niger on degrading ZEN (zearalenone) in the in-vitro environment of simulating the gastrointestinal tract, optimal degrading conditions of the fermentation product of aspergillus niger on ZEN are obtained, thereby providing a solid foundation for further developing the fermentation product of aspergillus niger into a biological antidote for degrading ZEN in the grain and feed.

The invention relates to an immunochromatographic test strip for synchronously detecting mixed pollution of aflatoxin and zearalenone, a preparation method and application thereof. The test strip includes a paperboard. An absorbent pad, a detection pad, a gold labeled pad and a sample pad are sticked on one side of the paperboard from top to bottom. Adjacent pads are in overlapping connection at joints. The detection pad adopts a nitrocellulose membrane as a base pad, which is provided with a transverse control line and detection lines. The two detection lines in internal distribution are positioned below the quality control line, and are respectively coated with a zearalenone-bovine serum albumin conjugate and an aflatoxin B1-bovine serum albumin conjugate. The quality control line is coated with a rabbit antimouse polyclonal antibody. The gold labeled pad is horizontally sprayed with a nanogold labeled anti-aflatoxin universal monoclonal antibody and a nanogold labeled anti-zearalenone monoclonal antibody. The immunochromatographic test strip can be used for simultaneously detecting the content of fungaltoxins aflatoxin and zearalenone in a sample, and has the characteristics of simple operation, rapidity, and high sensitivity.

The invention relates to a zearalenone hydrolase ZHD101 mutant and a method for hydrolyzing zearalenone by using the mutant. The mutant is obtained by introducing mutations at three key sites of the wild-type zearalenone hydrolase ZHD101. Compared with wild-type ZHD101, the ZHD101 mutant has the advantages that the specific activity for ZEN hydrolysis is improved by at least 75% under the acidic condition (pH 5.5); also, the catalytic efficiency (kcat / KM) for ZEN hydrolysis under acidic conditions (pH 5.5) can also be increased by as much as 40% compared with a wild-type ZHD101. Therefore, a foundation is laid for further improving the catalytic activity of the enzyme and realizing the industrial application under the acidic condition.

The invention discloses an acinetobacter strain and application thereof to degradation of zearalenone. The acinetobacter strain disclosed by the invention is Acinetobacter sp. SM04, and is preserved in the China General Microbiological Culture Collection Center on December 5th, 2011, and the preservation number is CGMCC No. 5524. The Acinetobacter sp. SM04 disclosed by the invention has stronger degradation capability towards mycotoxins ZEN, can degrade more than 98% of zearalenone into low-estrogenic-activity product within 36 hours, cannot produce high-estrogenic-activity analogues such as zearalenone and zeranol, and has real detoxification capability. The acinetobacter strain can be applied to the treatment of corn grains, corn alcohol residues or other mycotoxin contaminated feed, so that safe food and animal feed are obtained, and further, a zearalenone degradation strain which has low cost and high efficiency and avoids secondary pollution is provided for the environment-friendly processing and treatment of the grains and feed.