162 results about "Eimeria" patented technology

The present invention relates to a vaccine for coccidiosis in chickens prepared from four attenuated Eimeria species: E. acervulina, E. maxima, E. mitis and E. tenella. The vaccine was similar to or superior to other anticoccidial drugs in stimulating protective immunity against coccidiosis.

Vaccines comprising TRAP polypeptides and Salmonella enteritidis vectors comprising TRAP polypeptides are provided. The vaccines may also include a CD 154 polypeptide capable of binding to CD4Q. Also provided are methods of enhancing an immune response against Apicomplexan parasites and methods of reducing morbidity associated with infection with Apicomplexan parasites.

Vaccines comprising TRAP polypeptides and Salmonella enteritidis vectors comprising TRAP polypeptides are provided. The vaccines may also include a CD154 polypeptide capable of binding to CD40. Also provided are methods of enhancing an immune response against Apicomplexan parasites and methods of reducing morbidity associated with infection with Apicomplexan parasites.

Feeding yeast cell wall-containing compositions, including those compositions comprising mannanoligosaccharide(s), to animals exposed to or infected with coccidia, especially poultry exposed to pathogenic species of Eimeria, results in improved livestock performance and physical condition as compared with those animals who were not fed such compositions.

The present invention relates to a vaccine for coccidiosis in chickens prepared from three attenuated Eimeria species: E. acervulina, E. maxima and E. tenella. The vaccine was similar to or superior to other anticoccidial drugs in stimulating protective immunity against coccidiosis.

The invention discloses a method for batch preparation of chicken Eimeria coccidium oocysts, and particularly relates to a separation and purification method for producing oocysts (antigens) of chicken Eimeria coccidium vaccines. The method for preparing the chicken Eimeria coccidium oocysts comprises the following process steps: fecal sample processing, continuous screening, saturated brine float centrifuging, oocyst centrifugal depositing and oocyst purifying. According to the method, the industrial preparation of the chicken Eimeria coccidium oocysts can be realized, the production efficiency can be improved, and the cost can be reduced.

The invention discloses an Eimeria tenella AMA 1 gene, the nucleotide sequence of which includes a DNA sequence that codes the amino acid sequence represented by SEQ ID No.1 or a DNA sequence that codes the amino acid sequence which has AMA 1 protein activity and is obtained by omitting, adding, inserting or substituting one or more amino acid in the amino acid sequence represented by SEQ ID No.1. The invention also discloses proteins coded by the above-mentioned genes. The Eimeria tenella AMA 1 gene and proteins provided in the invention are closely related to invasion of daughter spores, escape and invasion of merozoites and escape and invasion of microgametes in the stage of sexual reproduction, and are suitable for being developed into novel vaccines or medicines for controlling coccidiosis.

The invention provides an immunoregulation type DNA vaccine with actions of preventing and treating chicken coccidiosis, which comprises calcium regulation bonding domain protein kinase genes pE+K2 of Eimeria tenella and chicken interleukin-2 genes, and a section of proteinase hydrolytic sequence is introduced to the 5' end of the chIL-2 gene.

The invention discloses an industrialized production method for separating and purifying a chicken eimeria coccidium oocyst. According to the industrialized production method, the separation and purification of the chicken eimeria coccidium oocyst are carried out by adopting the scientific assembly of a disc separator and a tubular type centrifuge, after sporulated oocysts are purified, larger impurities and extremely tiny impurities are not generated; an object plate is thoroughly subjected to microscopic examination, unsporulated oocysts are also all thoroughly removed, sporulated oocysts are all in the visual field, and the solution background is clear. The industrial production method disclosed by the invention can be used for shortening the separation and purification time of the oocysts; compared with the traditional technology, the industrial production method disclosed by the invention can be used for saving the time by more than 70%, reducing the product instability caused by excessive human factors by preventing integral oocyst purification from the disturbance of excessive artificial working and is the breakthrough of the existing coccidium vaccine production method; the industrial production method can be used for continuous production by only needing two centrifuges, thereby greatly reducing the cost input of an enterprise.

The invention discloses a novel tender Eimeria tenella protein kinase (EtPK) gene with a clone number of ZB1-A02 and the total length of 1684 bp, which contains an ORF with the length of 1527 bp and encodes 508 amino acids (a Genbank accession number is EF552216). The novel tender Eimeria tenella protein kinase (EtPK) gene is connected with a prokaryotic expression vector pGEX-4T-2 to establish a prokaryotic expression recombinant plasmid pGEX-4T-EtPK and is expressed in a colon bacillus system, and an expressed recombinant protein exists in a inclusion body way; a purified recombinant protein 4T-EtPK is processed by SDS-PAGE and then is transferred to a PVDF membrane, an antiserum of an E. tenella oocyst oral immune chicken is taken as first antibody, and a goat-anti chicken IgG is taken as second antibody to carry out Western-blot analysis, thereby the purified recombinant protein 4T-EtPK is shown to have certain antigenicity and can be used for developing chicken coccidiosis resistant medicament and preparing chicken coccidiosis resistant vaccines.

The present invention relates to a vaccine for coccidiosis in chickens prepared from three attenuated Eimeria species: E. acervulina, E. maxima and E. tenella. The vaccine was similar to or superior to other anticoccidial drugs in stimulating protective immunity against coccidiosis.

The invention relates to a method of vaccinating a domesticated bird against coccidiosis comprising administering in ovo an effective immnunizing dose of live Eimeria sporozoites or merozoites, or a mixture thereof. In a preferred embodiment, the domesticated bird that is vaccinated is a chicken or turkey.

Vaccine vectors and methods of using the vaccine vectors to enhance the immune response to an Apicomplexan parasite and reduce the morbidity or morality associated with subsequent infection are provided herein. The vaccine vectors include a polynucleotide encoding a Rhomboid polypeptide and optionally include an immune-stimulatory polypeptide suitably expressed on the surface of the vaccine vector.

The invention provides a new medicinal application of nitazoxanide and particularly discloses an application of nitazoxanide in preparing a drug for resisting eimeria coccidium. The application can serve for the prevention and treatment of coccidiosis of animals such as chickens and rabbits, and the like.

Vaccine vectors and methods of using the vaccine vectors to enhance the immune response to an Apicomplexan parasite and reduce the morbidity or morality associated with subsequent infection are provided herein. The vaccine vectors include a polynucleotide encoding a Rhomboid polypeptide and optionally include an immune-stimulatory polypeptide suitably expressed on the surface of the vaccine vector.

The invention discloses a method for extracting and purifying eimeria tenella merozoite. The method comprises the steps of: releasing the merozoite and purifying the merozoite, wherein the merozoite release comprises mechanical grinding release and enzymic digestion release, 0.0625% pancreatin and 0.175% sodium taurodeoxylate are adopted as digestive juice in the enzymic digestion release process; digestion is moderate, which is beneficial to the activation of the merozoite, can eliminate erythrocytes to obtain pure merozoite. The method is convenient and simple to operate, is low in cost, and is ideal in separation and purification effects; the separated merozoites are large in quantity, are pure with few impurities and keep vigorous energy; and the method is suitable for research on drug susceptibility detection, vaccine research and other biological researches.

The invention discloses an eimeria tenella Et CHP39 gene. The Et CHP39 gene includes an open reading frame nucleotide sequence represented as the SEQ ID No.1. The eimeria tenella conserved protein Et CHP39 gene is a new gene of the eimeria tenella and is relative to sporozoite invaded host cells. The eimeria tenella conserved protein Et CHP39 gene has quite high application value in development of a new vaccine or a new medicine capable of inhibiting the sporozoite invaded host cells and blocking a life history of the eimeria tenella.

The invention discloses a new Eimeria tenella heat shock protein 60 (Et HSP60) gene. The cloning number is ZB2-D07, the total length is 1802 bp, and the Et HSP60 gene contains a 1455 bp complete open reading frame (ORF) and can code 484 amino acids (Genbank accession number: FJ911605). The gene is connected with a prokaryotic expression vector pET28a(+) to construct a prokaryotic expression recombinant plasmid pET28a(+)-Et HSP60 and is expressed in an escherichia coli system. SDS-PAGE (Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis) is carried out by purifying the recombinant protein pET28a(+)-Et HSP60, and then the recombinant protein pET28a(+)-Et HSP60 is transferred to a PVDF (Polyvinylidene Fluoride) membrane. The antiserum of immuned chicken orally taking E. tenella oocyst is taken as a primary antibody, and goat-anti-chicken IgG (immunoglobulin G) is taken as a secondary antibody to carry out Western-blot analysis. The analysis result shows that the recombinant protein pET28a(+)-Et HSP60 has certain antigenicity and can be used for preparing a medicine for resisting chicken coccidiosis and a vaccine resisting chicken coccidiosis.

Antigenic polypeptides, capable of inducing an immune response against multiple parasites, and methods of designing such polypeptides, are provided. Also provided by the invention are polynucleotides encoding such polypeptides, as well as recombinant vectors and transformed host cells containing the said polynucleotides. Oral administration and intramuscular injection of the polypeptides provides vaccination protection against infection from Eimeria parasites that result in the poultry disease coccidiosis.

The invention provides an immunoregulation type DNA vaccine with actions of preventing and treating chicken coccidiosis, which comprises sporozoite surface antigen genes TA4 of Eimeria tenella and chicken interleukin-2 genes, and a section of proteinase hydrolytic sequence is introduced to the 5' end of the chIL-2 gene.

The invention provides a feed for treating eimeria tenella infection. The feed comprises the following raw materials: wheat, bean pulp, bone meal, rapeseed meal, monocalcium phosphate, vitamin E, vitamin K, pantothenic acid, lysine, methionine, threonine and a traditional Chinese medicine preparation for treating eimeria tenella coccidiosis. The traditional Chinese medicine preparation contained in the feed is low in drug resistance and generates few drug residues, has no toxic and side effect and has a good therapeutic effect of eimeria tenella coccidiosis.

The invention discloses an eimeria tenella Et CHP559 gene. The Et CHP559 gene includes an open reading frame nucleotide sequence represented as the SEQ ID No.1. The eimeria tenella conserved protein Et CHP559 gene is a new gene of the eimeria tenella and is relative to sporozoite invaded host cells. The eimeria tenella conserved protein Et CHP559 gene has quite high application value in development of a new vaccine or a new medicine capable of inhibiting the sporozoite invaded host cells and blocking a life history of the eimeria tenella.

The invention discloses an Eimeria tenella microcorticoid protein 2 related protein, and a preparation method and an application thereof. The protein is a newly discovered protein which is highly expressed by Eimeria tenella in each developmental stage. The amino acid sequence of the protein comprises an amino acid sequence represented by SEQ ID NO. 1. An EtM2AP gene is inserted into an Escherichia coli expression vector pET28a to obtain a recombinant expression vector, the recombinant expression vector is transferred into Escherichia coli to express a chicken Eimeria tenella recombinant protein (EtM2AP), and the recombinant protein is purified, and then is mixed with an adjuvant to prepare an EtM2AP recombinant protein vaccine. The vaccine can effectively increase the survival rate and the relative weight gain rate of chicks and reduce the coccidiosis oocyst discharge amount of the immunized chicks, and the EtM2AP recombinant protein vaccine has a good immune protection effect and hasthe potential of being used as a vaccine for preventing chicken Eimeria tenella coccidiosis.

The invention relates to a method for expressing an eimeria acevulina 3-1E protein in lactococcus lactis, and relates to a method for expressing an eimeria acevulina 3-1E protein in order to solve the problem that the eimeria acevulina sporozoite / merozoite stage surface antigen 3-1E can not be expressed in the lactococcus lactis. The method comprises the following steps of optimizing an eimeria acevulina 3-1E gene sequence according to the preference of the condon of the lactococcus lactis, inserting a cloning vector and transforming escherichia coli cells to obtain positive transformants; carrying out enzyme digestion and connection of the positive transformants and a lactic acid bacteria expression vector, and constructing a recombinant expression vector; transforming competent lactic acid bacteria cells, and identifying; and culturing and inducing recombinant bacteria to complete the expression of the 3-1E protein in the lactococcus lactis NZ9000. The artificially synthesized codon-optimized 3-1E protein is successfully expressed in the lactococcus lactis. The method provided by the invention is used for the prevention field of coccidiosis.

The invention discloses an eimeria tenella gene. The eimeria tenella gene is an eimeria tenella elF3d gene which can code an amino acid sequence shown in SEQ ID NO.1. The invention also discloses a protein coded by the eimeria tenella eIF3d gene. Recombinant protein coded by the eimeria tenella eIF3d gene has a good immune protection effect on avian coccidiosis and is applicable to being developed into a new vaccine or new drug which can be used for preventing and treating the avian coccidiosis.

The present invention provides improved methods and compositions for producing oocysts. The oocysts produced according to the invention find use in the manufacture of vaccines. In preferred embodiments, the present invention provides methods and compositions for the production of Eimeria oocysts. Vaccines containing Eimeria oocysts, sporocysts and / or sporozoites produced according to the present invention may be used to immunize birds against coccidiosis either in ovo or post hatch.

The invention discloses application of Vorinostat in preparation of soft-resistant Eimeria drugs; powder is formed by the Vorinostat and an acceptable auxiliary material, and then is added into a feed to mix uniformly so as to feed chicken by a daily feed amount; or a Vorinostat water-soluble powder is prepared by the Vorinostat, and is dissolved in water to feed chicken by a daily feed amount. Animal infection experiments confirm an anticoccidia index (ACI) of the Vorinostat is more than 175, and the Vorinostat has function of resisting soft-resistant Eimeria.

The invention provides an eimeria tenella AN1-like Zinc finger domain-containing protein and an application thereof in inhibiting invasion of coccidium. The eimeria tenella AN1-like Zinc finger domain-containing protein is cloned, expressed and purified. The transcription level of EtAN1-ZnFP in different developmental stages of polypides is detected by fluorescence quantitative PCR (qPCR). The immune protective effect of EtAN1-ZnFP after chickens are infected with coccidium is evaluated by immunoprotection experiments. Research results indicate that the EtAN1-ZnFP is possible to participate ininvasion and growing development of eimeria tenella.

The invention relates to a chicken coccidiosis prevention and treatment preparation, in particular to a preparation technology of a chicken Eimeria tenella egg yolk antibody targeted sustained release preparation so as to solve the prevention and treatment problems of chicken coccidiosis. The preparation technology of the chicken Eimeria tenella egg yolk antibody targeted sustained release preparation includes the steps of: preparation, separation and purification of a highly immunized egg yolk antibody (IgY), and preparation of a microcapsule. Preparation of the microcapsule has the reaction conditions that: the chitosan concentration is 0.05-0.8% (w / v), the concentration of CaCl2 is 0.5-1.5% (w / v), the concentration of sodium alginate is 1.0-3.0% (w / v), and the IgY and the sodium alginate are in a mass ratio of 1-4:8. The IgY microcapsule prepared by the technical scheme involved in the invention is a preventive medicine, and is also a therapeutic drug. The microcapsule prepared by the invention provides a very good example for antibiotic substitution therapy. And the determined technology can also be used to prepare IgY or IgG microcapsules of other pathogenic microorganisms, thus having promotional value, and being beneficial to sound development of the green and organic chicken raising industry.

The invention discloses a preparation method and application of an anticoccidial traditional Chinese medicine composition. Dried dandelions and dried fructus cnidii are obtained and put into a traditional Chinese medicine grinding machine for grinding for 10-20 minutes, the materials are sieved through a 100-mesh screen, and dandelion powder and fructus cnidii powder are obtained; the dandelion powder, the fructus cnidii powder and salt are mixed in the mass ratio of (50-60):(30-40):(1-10), and the anticoccidial traditional Chinese medicine composition is obtained. The preparation process is simple and reliable, the medicine materials in use come from plants, planting and processing of the medicine materials are environmentally friendly, the prepared medicine is small in toxic and side effect on animals in use and free of irritation, antibiotic residues in animal food are avoided, and food safety is guaranteed. The prepared traditional Chinese medicine composition has the effect of resisting infection of one kind of eimeria coccidium and mixed infection of various kinds of eimeria coccidium, and is novel traditional Chinese medicine powder for preventing coccidiosis. Pharmacodynamical research shows that the traditional Chinese medicine composition has an obvious prevention and treatment effect on chick coccidiosis.