78 results about "Cryptosporidium" patented technology

The present invention provides a multiplex RT-PCR/PCR method, which enables in a single assay the simultaneous detection of any combination of bovine rotavirus, bovine coronavirus, Cryptosporidium parvum, and optionally, Escherichia coli strains producing K99 pili or heat-stable enterotoxin STa.

The invention discloses a combined processing technology for slightly polluted raw water. The raw material water firstly enters into a first ozone contact tank for pre-ozonation, then enters into a flocculation sedimentation tank for sedimentation treatment, after sedimentation, the water enters into a secondary ozone contact tank for ozonation decomposition, then enters into a carbon sand filtering tank for filtering, the filtered water enters into a membrane processing system, the membrane-processed water enters into a clean water tank for dispatching and storing and are disinfected in the clean water tank by adding chlorine, and the water enters into a suction well and is conveyed to a water distribution network via a pump set in a clean water pump house. The combined processing technology of the invention has the advantages that: the finished water coming from the processed slightly polluted raw water can not only be guaranteed to reach the sanitary standard for drinking water, but also meet higher requirements in some indexes, and is eliminated in microbial overweight risk and effectively removed in humic acid and cryptosporidium and giardia. Additionally , compared with the prior art, the combined processing technology combined with advanced treatment process helps to saves land occupations of a sand filtering tank and a disinfection contact tank and enable water quality upgrading construction of an existing water factory with a compact field to be implemented.

The present invention relates to fusion proteins comprising a microorganism targeting molecule (e.g., immunoglobulin) and a biocide. The present invention also relates to therapeutic and prophylactic methods of using a fusion protein comprising a microorganism targeting molecule and a biocide in diverse fields.

The present invention describes novel oligonucleotides targeted to nucleic acid sequences derived from Cryptosporidium organisms, and Cryptosporidium parvum organisms in particular, which are useful for determining the presence of Cryptosporidium organisms in a test sample. The oligonucleotides of the present invention include hybridization assay probes, helper probes and amplification primers. The present invention further describes a novel method for obtaining purified ribonucleic acid from viable oocysts.

One aspect of the present invention relates to compounds, and pharmaceutically acceptable salts and prodrugs thereof, that are useful as inhibitors of IMPDH. The invention also provides pharmaceutical compositions comprising the compounds of the invention which selectively inhibit parasitic IMPDH. In certain embodiments, the present invention relates to selective inhibition of C. parvum inosine-5′-monophosphate-dehydrogenase over human inosine-5′-monophosphate-dehydrogenase (IMPDH type I and type II). These compounds may be used alone or in combination with other therapeutic or prophylactic agents, such as anti-virals, anti-inflammatory agents, antimicrobials and immunosuppressants.

The invention provides a method for detecting cryptosporidium parvum and a detection kit. The detection method comprises the following steps: (1) separating and purifying a sample to be detected by immunomagnetic beads to prepare suspension containing cryptosporidium parvum; and (2) detecting the suspension containing cryptosporidium parvum prepared in the step (1) by fluorogenic quantitative PCR (polymerase chain reaction). The detection kit comprises a reaction system containing biotinylated cryptosporidium parvum Cp23 monoclonal antibody coated streptomycin magnetic beads in the detection method, a specific forward primer of cryptosporidium parvum, a specific reverse primer and a Taqman fluorogenic quantitative probe primer. According to the cryptosporidium parvum detection method, the sensitivity is 100 times higher than that of a traditional method, and is far beyond that of the traditional method.

This invention relates to a method for enhanced sanitation and oxidation of aqueous solutions at aquatic facilities. The method provides a means for the in-situ generation of chlorine dioxide from dilute solutions of chlorite anions at near neutral pH, and enhanced inactivation rates of microbiological organisms including cryptosporidium.

The invention provides a cryptosporidium bivalent DNA vaccine and a preparation method thereof, which use a eucaryon expression carrier pVAX1 as a carrier, and combine two cryptosporidium protective antigen genes and a CPG immune strengthening sequence inserted into a polyclonal site region of the pVAX1 eucaryon expression carrier in series to obtain the cryptosporidium bivalent DNA vaccine containing the immune strengthening sequence. Two protective antigen genes are chosen to carry out the development of the bivalent DNA vaccine, and the CPG immune strengthening sequence is introduced on the basis, thus enhancing the immune protective effect. The vaccine has stronger cell immunity and humoral immunity functions, and the introduced CPG motif has the functions of invigorating the immunity system, inducing the natural immunity response, and secreting immune globulin and various cell factors, and the two are in the superiority combination, thus achieving the aim of better preventing animal cryptosporidium diseases.

The invention discloses a bovine Cryptosporidium ELISA detection kit. The kit comprises a coated ELISA plate, a negative standard serum, a positive standard serum, an enzyme-labeled second antibody, a washing liquid, a diluent, a substrate liquid and a stopping liquid, and the coated ELISA plate treats a concatermer COWP-HSP70 of COWP and a heat shock protein HSP70 as a coating antigen. The fusion interaction of the above two proteins is discussed through the series expression of the two proteins, and a coating antigen having a strong immunogenicity is obtained for the first time in the world because of the approximation to the self condition of the polypide. The kit and an ELISA method established in the invention are helpful for the deep development of the immunoprophylaxis and immunodiagnosis value researches of the bovine Cryptosporidium, and provide a necessary technical means for the fast detection and diagnosis of the bovine Cryptosporidium.

The invention relates to a kit capable of rapidly detecting the cryptosporidium in food. The kit mainly solves the technical problems of the absence of a detecting method at both home and abroad and too long detection time. The kit capable of rapidly detecting the cryptosporidium in food consists of the following agents: an agent A: a fixed-DNA FTA card consisting of an FTA card, an FTA purifying reagent and an FTA cleaning reagent (TE buffer:0.01mol/L Tris, pH 8.0; 0.1 mmol/L EDTA); an agent B: PCR reaction liquor containing two specific primer pairs of CryoutL and CryoutR which respectively have the sequences of 5'-TTCTAGAGCTAATACATGCG-3' and 5'-CCCACTT CTTCGAAGCAGGA-3'; an agent C: PCR reaction liquor containing two specific primer pairs of CryinL and CryinR which respectively have the sequences of 5'-GGAAGGGTTGTATTTATTAGATAAAG-3' and 5'- CTCATAAGGTGCTGAAGGAGTA-3'. The method is used to detect the cryptosporidium in a sample.

The invention discloses a primer set for detecting oocyst deoxyribonucleic acid (DNA) of Cryptosporidium by using a loop-mediated isothermal amplification technology. The primer set comprises an external primer pair (F3,B3) and an internal primer pair (FIP,BIP), wherein F3 has a sequence shown in SEQ ID No: 1 or a complementary strand of the sequence; B3 has a sequence shown in SEQ ID No: 2 or a complementary strand of the sequence; FIP has a sequence shown in SEQ ID No: 3 or a complementary strand of the sequence; and BIP has a sequence shown in SEQ ID No: 4 or a complementary strand of the sequence. The invention also discloses a method for detecting the oocyst DNA of the Cryptosporidium by performing a loop-mediated isothermal amplification reaction by using the primer set, a kit for detecting the oocyst DNA of the Cryptosporidium, and application of the primer set to the preparation of a detection agent of the oocyst DNA of the Cryptosporidium. In the invention, the rapid detection of the oocyst DNA of the Cryptosporidium is realized by designing two pairs of specific primers of the Cryptosporidium, so that the speed and the accuracy rate of clinical diagnosis of cryptosporidiosis are improved.

Combination compositions including C. parvum antigen(s) or epitope(s) of interest with at least one other antigen or epitope of interest from a pathogen that causes enteric infection and/or symptoms and/or recombinant(s) and/or vector(s) and/or plasmid(s) expressing such antigen(s) or epitope(s) of interest and administration of such compositions such as to pregnant mammals and/or newborn or young mammals, for instance, pregnant cows and/or calves such as within the first month of birth, are disclosed and claimed.

The invention relates to a preparation method of an activated carbon filter. The preparation method includes the steps: (1) preparing raw materials: putting powdered activated carbon and powdered activated zeolite into a raw material pool, putting an adhesive into the raw material pool, injecting water according to a weight ratio, then putting a synthetic fiber into the raw material pool, stirring, and wrapping a porous plastic pipe with a two-layer non-woven fabric; (2) adopting a suction molding method: allowing the raw materials in a tank to gather on the porous plastic pipe, and eventually filling fully a whole suction and injection device; and (3) adopting an injection molding method: making the raw materials gather in an injection metal mold with the porous plastic pipe as the axle center, and eventually filling fully the whole injection metal mold. The beneficial effects are that the activated carbon filter can effectively remove peculiar smell and free organic compounds in water, and also can remove trace heavy metals such as cadmium, lead, chromium and the like in a liquid; no activated carbon and other adsorption media fall off, and thus secondary pollution is effectively avoided; through a precise mesh-number matching of activated carbon, the removal rate of cryptosporidium is high; and the liquid flow rate is increased, and the resistance is decreased.

The invention relates to double-antibody sandwiched colloidal gold immunochromatographic test paper for detecting zoonotic cryptosporidia. The double-antibody sandwiched colloidal gold immunochromatographic test paper prepared on the basis of CSpV viruses is used for detecting the zoonotic cryptosporidia; the specificity and the sensitivity are high, and by a double-antibody sandwiched method using a CSpV monoclonal antibody and a CSpV polyclonal antibody, the monoclonal antibody guarantees the specificity of the method and the polyclonal antibody improves the sensitivity of the double-antibody sandwiched colloidal gold immunochromatographic test paper, so that a detection result is more accurate. The prepared cryptosporidium detection colloidal gold test paper has the characteristics of rapidness, convenient operation, no need of a special instrument and the like; the detection result is clear and can be judged relatively quickly; and the double-antibody sandwiched colloidal gold immunochromatographic test paper is suitable for rapid diagnosis of a clinical site and large-scale epidemiological investigation in a pastoral area and the like.

The invention discloses a method for purifying cryptosporidium protein kinase. Firstly, an in-vitro recombination expression vector of cryptosporidium protein kinase is constructed; then plasmid transduction and IPTG low-temperature inducible expression are carried out; after successfully expressed thallus is split, protein is purified through rubber tapping recycling. According to the method forpurifying cryptosporidium protein kinase, by means of low-temperature inducing and IPTG concentration improving, the solubility of expression protein is improved, the problem that during in-vitro expression of cryptosporidium protein kinase, inclusion body expression is likely to autotomizing degradation is solved through a rubber tapping recycling method, high-purity single-strip recombinant protein with enzyme activity can be obtained, and the purifying effect on protein hard to express and large in molecular weight can be improved. The purified protein can be further used for functional analysis and studies, and a foundation is laid for solving various problems in the field of cryptosporidium protein functions.

The present invention provides yolk immunoglobulin preparation for preventing and treating cryptosporidiosis and health product of yolk immunoglobulin for strengthening immunity. The yolk immunoglobulin is prepared through the following steps: gathering cryptosporidium egg capsule from positive cryptosporidium excrement, separating, sucrose gradient centrifuging to purify cryptosporidium egg capsule and prepare cryptosporidium antigen, immunizing female laying fowl with the cryptosporidium antigen, collecting laid fowl egg, collecting yolk, diluting with distilled water, centrifuging to obtain supernatant, water diluting, and salting out with ammonium sulfate to obtain yolk immunoglobulin. The present invention is acted on the pathogen of cryptosporidiosis to reach specific inhibition.

The invention provides a fast detecting and source-tracing method for cryptosporidium and giardia lamblia in urban sewage. The method comprises the following steps of: 1) collecting a proper amount of urban sewage, leaving the urban sewage for a while, abandoning sewage in the upper layer and collecting sewage in the lower layer; 2) repeating the step 1) to a certain water sample total amount; 3) centrifugalizing the collected water sample and collecting a precipitate; 4) extracting DNA in the precipitate; 5) performing PCR and sequencing on the extracted DNA to detect cryptosporidium and giardia lamblia in the urban sewage; and 6) comparing the sequence obtained in the step 5) with a sequence in the GenBank to determine the pest species or genotypes. By virtue of a method of performing PCR on the sewage after centrifugal precipitation, the invention provides the fast detecting and source-tracing method for cryptosporidium and giardia lamblia in the urban sewage, wherein the method is small in demand in sample volume, simple to operate, accurate in result, ideal in species-fixing and source-tracing effect and low in cost.

The invention relates to a CTL epitope peptide of cryptosporidium parvum. The peptide has the amino acid sequence of SEQ ID NO.1. The invention further provides a nucleic acid for coding the CTL epitope peptide of cryptosporidium parvum. The nucleic acid has the nucleotide sequence of SEQ ID NO.2. The CTL epitope peptide P1 of cryptosporidium parvum has quite high affinity to H-2Kb molecules or HLA-A*0201 molecules, and it means that the CTL epitope peptide has the corresponding immunology functions. A new direction is provided for preparing a cryptosporidium parvum vaccine. The peptide presents excellent irritation reactions in the polypeptide specificity CTL immune reaction in C57BL/6N mouse splenocytes and has huge development and application potential in the cryptosporidium parvum specific immunotherapy field.