Kit for quickly detecting cryptosporidium

A detection kit and technology for Cryptosporidium, which can be used in the determination/inspection of microorganisms, resistance to vector-borne diseases, biochemical equipment and methods, etc., and can solve the problems of long and cumbersome detection
CN101289689AInactive Publication Date: 2008-10-22SHANGHAI ENTRY EXIT INSPECTION & QUARANTINE BUREAU OF P R C
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
SHANGHAI ENTRY EXIT INSPECTION & QUARANTINE BUREAU OF P R C
Publication Date
2008-10-22
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention relates to a kit capable of rapidly detecting the cryptosporidium in food. The kit mainly solves the technical problems of the absence of a detecting method at both home and abroad and too long detection time. The kit capable of rapidly detecting the cryptosporidium in food consists of the following agents: an agent A: a fixed-DNA FTA card consisting of an FTA card, an FTA purifying reagent and an FTA cleaning reagent (TE buffer:0.01mol / L Tris, pH 8.0; 0.1 mmol / L EDTA); an agent B: PCR reaction liquor containing two specific primer pairs of CryoutL and CryoutR which respectively have the sequences of 5'-TTCTAGAGCTAATACATGCG-3' and 5'-CCCACTT CTTCGAAGCAGGA-3'; an agent C: PCR reaction liquor containing two specific primer pairs of CryinL and CryinR which respectively have the sequences of 5'-GGAAGGGTTGTATTTATTAGATAAAG-3' and 5'- CTCATAAGGTGCTGAAGGAGTA-3'. The method is used to detect the cryptosporidium in a sample.
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Description

Technical field:

[0001] The invention relates to a detection device for detecting parasites in food, in particular to a kit for rapidly detecting cryptosporidium in food. Background technique:

[0002] Cryptosporidium (Cryptosporidium) belongs to the phylum Apicomplexa, the class Sporidia, the subclass Coccidia, the order Eucoccidia, the suborder Eimeria, the family Cryptosporidium, and the genus Cryptosporidium. It is an extremely small single-cell intestinal parasite with a diameter of 3.2 μm to 5.1 μm×8.0 μm to 8.5 μm, which cannot be recognized by the naked eye. Cryptosporidium parasitizes in the gastrointestinal tract epithelium and respiratory epithelial microvilli of humans or animals, and its cryptosporidiosis is an important zoonotic parasitic disease. So far, 13 effective species of Cryptosporidium have been recognized. Before the 1970s, humans only knew that Cryptosporidium was an intestinal parasite, which could infect dozens of animals such as cattle, horses, ...

Claims

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