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Kit for quickly detecting cryptosporidium

A detection kit and technology for Cryptosporidium, which can be used in the determination/inspection of microorganisms, resistance to vector-borne diseases, biochemical equipment and methods, etc., and can solve the problems of long and cumbersome detection

Inactive Publication Date: 2008-10-22
SHANGHAI ENTRY EXIT INSPECTION & QUARANTINE BUREAU OF P R C
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to provide a kit and detection method for rapid detection of Cryptosporidium in food samples, which mainly solves the technical problems that there is no detection standard method in food samples at home and abroad and the detection time is too long and cumbersome.

Method used

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  • Kit for quickly detecting cryptosporidium
  • Kit for quickly detecting cryptosporidium
  • Kit for quickly detecting cryptosporidium

Examples

Experimental program
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Effect test

Embodiment Construction

[0040] Beverage samples were tested with a kit for rapid detection of Cryptosporidium in samples. The kit consists of:

[0041] Reagent A: It consists of: FTA card for immobilizing DNA (the patented product of Whatman Company in the United States); FTA purification reagent (subpackaged by Whatman Company); FTA washing reagent (TE buffer: 0.01mol / L Tris, pH 8.0; 0.1mmol / L EDTA). Reagent A relates to US patents US5496562, US5756126, US5807527, US5972386, US5985327.

[0042] Reagent B: PCR reaction solution, which contains two specific pairs of primers CryoutL and CryoutR, the sequences of which are: 5'-TTCTAGAGCTAATACATGCG-3' and 5'-CCCACTT CTTCGAAGCAGGA-3' respectively.

[0043] Reagent C: PCR reaction solution, which contains two specific pairs of primers CryinL and CryinR, the sequences of which are: 5'-GGAAGGGTTGTATTTATTAGATAAAG-3' and 5'-CTCATAAGGTGCTGAAGGAGTA-3', respectively.

[0044] According to the use method of the cryptosporidium kit in the rapid detection sample p...

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Abstract

The invention relates to a kit capable of rapidly detecting the cryptosporidium in food. The kit mainly solves the technical problems of the absence of a detecting method at both home and abroad and too long detection time. The kit capable of rapidly detecting the cryptosporidium in food consists of the following agents: an agent A: a fixed-DNA FTA card consisting of an FTA card, an FTA purifying reagent and an FTA cleaning reagent (TE buffer:0.01mol / L Tris, pH 8.0; 0.1 mmol / L EDTA); an agent B: PCR reaction liquor containing two specific primer pairs of CryoutL and CryoutR which respectively have the sequences of 5'-TTCTAGAGCTAATACATGCG-3' and 5'-CCCACTT CTTCGAAGCAGGA-3'; an agent C: PCR reaction liquor containing two specific primer pairs of CryinL and CryinR which respectively have the sequences of 5'-GGAAGGGTTGTATTTATTAGATAAAG-3' and 5'- CTCATAAGGTGCTGAAGGAGTA-3'. The method is used to detect the cryptosporidium in a sample.

Description

Technical field: [0001] The invention relates to a detection device for detecting parasites in food, in particular to a kit for rapidly detecting cryptosporidium in food. Background technique: [0002] Cryptosporidium (Cryptosporidium) belongs to the phylum Apicomplexa, the class Sporidia, the subclass Coccidia, the order Eucoccidia, the suborder Eimeria, the family Cryptosporidium, and the genus Cryptosporidium. It is an extremely small single-cell intestinal parasite with a diameter of 3.2 μm to 5.1 μm×8.0 μm to 8.5 μm, which cannot be recognized by the naked eye. Cryptosporidium parasitizes in the gastrointestinal tract epithelium and respiratory epithelial microvilli of humans or animals, and its cryptosporidiosis is an important zoonotic parasitic disease. So far, 13 effective species of Cryptosporidium have been recognized. Before the 1970s, humans only knew that Cryptosporidium was an intestinal parasite, which could infect dozens of animals such as cattle, horses, ...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12Q1/04
CPCY02A50/30
Inventor 李晓虹阎东丽
Owner SHANGHAI ENTRY EXIT INSPECTION & QUARANTINE BUREAU OF P R C
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