Bovine Cryptosporidium ELISA detection kit

A technology for detecting kits and sporozoites, which can be used in biological testing, material inspection products, etc., and can solve the problems of unstable effect and inability to eradicate cryptosporidium.

Active Publication Date: 2013-10-23
GUANGXI VETERINARY RES INST
View PDF6 Cites 6 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Although some antiparasitic drugs, antiviral drugs and antibiotics have been used to study the effect of anti-Cryp...

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Bovine Cryptosporidium ELISA detection kit
  • Bovine Cryptosporidium ELISA detection kit
  • Bovine Cryptosporidium ELISA detection kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0023] The research process of tandem expression of oocyst wall protein and heat shock protein, identification of immunogenicity and establishment of ELISA detection method in the present invention will be described in detail below. Among them, skimmed milk powder Difco TM Skim milk was purchased from Solarbio; enzyme-labeled secondary antibody: goat anti-mouse-Ig G was purchased from Zhongshan Jinqiao Company; other reagents were domestic analytically pure products.

[0024] 1. Preparation of Coating Antigen

[0025] Select recombinant strains pET-32a-COWP and pET-32a-HSP70 with correct sequencing results and containing oocyst wall protein gene and heat shock protein gene respectively for mass culture, extract plasmids, and double-enzyme digest the extracted products, and enzymes at 37°C Cut for 8 hours or overnight; perform gel electrophoresis after recovery with a gel recovery kit to determine the amount of the vector and the target gene. The molar ratio of the vector DN...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a bovine Cryptosporidium ELISA detection kit. The kit comprises a coated ELISA plate, a negative standard serum, a positive standard serum, an enzyme-labeled second antibody, a washing liquid, a diluent, a substrate liquid and a stopping liquid, and the coated ELISA plate treats a concatermer COWP-HSP70 of COWP and a heat shock protein HSP70 as a coating antigen. The fusion interaction of the above two proteins is discussed through the series expression of the two proteins, and a coating antigen having a strong immunogenicity is obtained for the first time in the world because of the approximation to the self condition of the polypide. The kit and an ELISA method established in the invention are helpful for the deep development of the immunoprophylaxis and immunodiagnosis value researches of the bovine Cryptosporidium, and provide a necessary technical means for the fast detection and diagnosis of the bovine Cryptosporidium.

Description

technical field [0001] The invention relates to the technical field of bovine cryptosporidium detection, in particular to an ELISA detection kit for bovine cryptosporidium. Background technique [0002] The research on co-expression in bacteria and viruses is mainly to express virulence genes in series, which can improve the immunogenicity of the target protein, and prepare it into a vaccine to obtain a higher protection rate; or combine two segments of target genes from different sources Together, two proteins from different sources can be obtained at the same time, and the target protein can be made into a vaccine for immunization, so that animals can produce antibodies from two different pathogens, thus effectively defending against the invasion of the two pathogens. For example, it has been reported that the highly conserved N gene of porcine reproductive and respiratory syndrome virus and the HA gene of influenza virus were jointly expressed, and the hemagglutinin HA ge...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): G01N33/68
Inventor 彭昊唐林生李军杨威陶立谢永平潘艳韦志锋秦若甫兰美益禤雄标胡帅谢宇舟马春霞陈泽祥
Owner GUANGXI VETERINARY RES INST
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap