252 results about "Elisa method" patented technology

The invention discloses a monoclonal antibody for distinguishing a hog cholera lapinized virus vaccine strain and a hog cholera lapinized virus epidemic strain and application of the monoclonal antibody, and belongs to the field of immune globulin. The E2 protein monoclonal antibody or the antigen binding fragment thereof comprises a heavy chain variable region (SEQ ID No.1) and a light chain variable region (SEQ ID No.3). The invention also discloses an ELISA method for identifying and distinguishing hog cholera lapinized virus vaccine strains and epidemic strains by using the E2 protein monoclonal antibody. The monoclonal antibody disclosed by the invention has a specific reaction with the swine fever virus epidemic strain and does not react with the swine fever lapinized virus vaccine strain, and the swine fever lapinized virus vaccine strain and the epidemic strain can be accurately identified and distinguished by utilizing an ELISA competition method established by the monoclonal antibody. The monoclonal antibody provided by the invention can be used in the fields of CSFV immunization, serological diagnosis of wild viruses, immune effect evaluation, related experiments and the like, and provides antibody resources for effective detection and purification of CSFV.

The invention belongs to biotechnology, and particularly relates to an African swine fever virus p30 protein monoclonal antibody as well as a preparation method and application thereof. The main technology comprises the following steps: immunizing a BALB / c mouse by using a recombinant p30 protein prepared by prokaryotic expression as an antigen, and carrying out cell fusion, indirect ELISA method screening, limited dilution method subcloning and hybridoma continuous subculture by using a hybridoma technology to obtain a hybridoma cell line capable of stably secreting an anti-African swine fever virus p30 protein monoclonal antibody. The invention also discloses a sequence of an immunogen of the prepared monoclonal antibody, a subtype of the monoclonal antibody, a CDR sequence, a preservation number of a hybridoma cell strain and application of the monoclonal antibody in a Western Blot experiment, an indirect immunofluorescence experiment, a neutralization experiment and a blocking ELISA method for detecting the African swine fever virus antibody in swine serum.

The invention discloses a broad-spectrum monoclonal antibody for resisting porcine epidemic diarrhea virus S protein and application of the broad-spectrum monoclonal antibody. Specifically, firstly, two monoclonal antibodies GC33D8-1 and GC33A4-3 which have broad-spectrum binding characteristics with the porcine epidemic diarrhea virus S protein are obtained, the GC33D8-1 antibody has good universality and can have strong affinity with different strains, and the GC33A4-3 antibody can be bound for 3 times. Secondly, the two monoclonal antibodies are respectively used as a capture antibody and a detection antibody, a double-antibody sandwich ELISA method is established, and the double-antibody sandwich ELISA method is applied to PEDV vaccine S protein quantification, has better parallelism compared with other antibodies, and can be widely applied to detection of different strains. The method can be used for detecting semi-finished products and finished products, can be used for process establishment and optimization of porcine epidemic diarrhea virus vaccine antigens, vaccine quality evaluation and stability monitoring, has the advantages of high sensitivity, high accuracy, high repeatability and high flux, and solves the problems that broad-spectrum detection cannot be carried out at present and an S protein ELISA method only can be used for qualitative judgment.

This invention relates to the field of giant panda reproductive technology, and in particular to a method for predicting true pregnancy in giant pandas. Based on the physiological characteristic that the mother's immunity gradually decreases during pregnancy due to reproductive stress, this invention predicts true pregnancy in female giant pandas. It can accurately and scientifically determine whether a mated giant panda is truly pregnant, and the prediction results can be obtained 1-2 weeks before the actual delivery date. This is significant for making early changes to the feeding and management of truly pregnant giant pandas, the formulation and implementation of newborn panda delivery procedures, and the optimization of reproductive management in captive giant pandas. Furthermore, the prediction method provided by this invention enriches the methods for distinguishing between true and false pregnancies in giant pandas by examining pregnancy metabolites. Because it uses the ELISA method, it eliminates the need for professional training in ultrasound diagnosis and the need to acclimatize mated giant pandas, saving significant manpower and resources while simplifying the operational procedures.

The invention discloses an African swine fever virus p30 protein antibody and application thereof. When the African swine fever virus p30 protein antibody is used for a competitive ELISA method, a serum sample with the p30 antibody after African swine fever virus infection can be rapidly and accurately detected, the detection time is shortened, the detection steps are simplified, the African swine fever virus p30 protein antibody has the characteristics of rapidness and high-throughput detection, and a reliable immunological technology is provided for rapidly and efficiently detecting African swine fever virus infection.

The invention belongs to the field of immunological detection, and provides an anti-human albumin antibody 5H11 and application thereof. The anti-human albumin antibody provided by the invention can be specifically combined with human albumin and has relatively high stability. Based on the antibody, a kit capable of accurately, rapidly and massively detecting the content of HSA in a sample is developed. The double-antibody sandwich ELISA method for detecting the human albumin has high sensitivity, specificity and accuracy, can realize accurate and high-throughput detection of the content of albumin in human serum or other serum-like species containing the human albumin, and has a good application prospect in detection of the human albumin.

The invention belongs to the technical field of biological detection, and particularly relates to a monoclonal antibody 1D3 for detecting a porcine epidemic diarrhea virus antibody and application of the monoclonal antibody 1D3. The CDR sequences of the heavy chain variable region are respectively shown as SEQ ID NO.1-3, and the CDR sequences of the light chain variable region are respectively shown as SEQ ID NO.4-6. The antibody can specifically recognize PEDV / S1 protein, binding of the antibody can be competitively blocked by a PEDV specific neutralizing antibody in serum, and the antibody is suitable for establishing a blocking ELISA method. A detection tool based on the antibody has high specificity and sensitivity, and the blocking rate to PEDV positive serum is higher than 50%. The invention can be used for preparing a detection kit or evaluating the immune effect of a vaccine, and provides an efficient and accurate technical means for PEDV infection diagnosis and immune monitoring.

The invention relates to the technical field of molecular biology, in particular to an avian adenovirus serum type 4 colloidal gold test strip based on a monoclonal antibody and application of the test strip. According to the invention, two monoclonal antibodies (6B3 and 8G11) aiming at FAdV4 penton protein are successfully prepared through a hybridoma technology, and both the monoclonal antibodies show high titer and strong serotype specificity. Specific analysis proves that the two monoclonal antibodies can recognize 12 FAdV4 isolates from different sources, and do not have cross reaction with other FAdV serotypes or common poultry pathogens. According to the invention, through systematic optimization of key parameters, the detection sensitivity of the test strip is 7.81 * 10 < 4 > TCID50 / 0.1 mL, and the detection time is only 15 minutes. Clinical sample verification shows that compared with an ELISA method, the total coincidence rate is 98.6%.

The invention belongs to the technical field of biochemical engineering, and particularly relates to an amitriptyline hapten and a preparation method thereof, an amitriptyline antigen and a preparation method thereof, an antibody, a kit and application. The amitriptyline hapten has a structural formula shown as a formula (I) or a formula (II). Compared with an existing structure, the amitriptyline hapten prepared by the invention has a better immune effect. The obtained antibody is high in titer, strong in specificity and high in affinity, the LOD of amitriptyline by using an Elisa method established by the antibody is 0.013 ng / mL, the IC50 of amitriptyline is 0.865 ng / mL, the quantitative detection range is 0.07-470.3 ng / mL, the detection sensitivity is high, and the linear range is wide.

This invention belongs to the field of biodetection technology, specifically relating to a monoclonal antibody 1D3 for detecting porcine epidemic diarrhea virus (PEDV) and its applications. Its heavy chain variable region CDR sequences are SEQ ID NO. 1-3, and its light chain variable region CDR sequences are SEQ ID NO. 4-6. This antibody specifically recognizes the PEDV / S1 protein, and its binding can be competitively blocked by PEDV-specific neutralizing antibodies in serum, making it suitable for establishing a blocking ELISA method. Detection tools based on this antibody exhibit high specificity and sensitivity, with blocking rates exceeding 50% against PEDV-positive serum. This invention can be used to prepare detection kits or evaluate vaccine immunization efficacy, providing an efficient and accurate technical means for PEDV infection diagnosis and immune monitoring.

The invention provides a method for detecting a virus inactivation effect by an ELISA (Enzyme-Linked Immunosorbent Assay) method, which comprises the following steps: acquiring a to-be-detected inactivated virus sample, extracting an antigen component from the sample, and detecting an antigen structure change characteristic value through a high-throughput mass spectrometry analysis technology to obtain antigen degradation degree data; obtaining a sample subjected to preliminary inactivation, and detecting the antigen content change trend through an enzyme-linked immunosorbent assay to obtain content change curve data; detecting the residual quantity of viral nucleic acid by a real-time fluorescent quantitative PCR (Polymerase Chain Reaction) technology aiming at the immunogenicity retained sample to obtain nucleic acid degradation degree data; acquiring nucleic acid degradation degree data, classifying the relationship between nucleic acid residues and infectivity by adopting a support vector machine algorithm, and judging that the inactivation effect is completely completed if the classification result shows that the infectivity is lost; according to the antigen content change trend, the immunogenicity index and the nucleic acid degradation degree data, a weighted fusion algorithm is adopted to integrate multi-dimensional detection results, and a comprehensive inactivation effect score is obtained.

The invention discloses a human IL-13 antibody, an ELISA kit containing the human IL-13 antibody and a detection method of the human IL-13 antibody. Specifically, the ELISA kit provided by the invention comprises a pair of anti-human IL-13 monoclonal antibodies which are respectively used as a coating antibody and a detection antibody in a double-antibody sandwich ELISA method. The kit can be used for successfully completing quantitative detection of human IL-13, has relatively high detection sensitivity and good detection specificity, can be used for detecting a plurality of species of IL-13, and has good development and application prospects.

The invention discloses a monoclonal antibody and application thereof in quantitative detection of porcine epidemic diarrhea virus, and belongs to the technical field of biological detection. The preparation method comprises the following steps: firstly, obtaining two monoclonal antibodies GC44F3-1 and GC32C11-5 which specifically react with S protein of the porcine epidemic diarrhea virus; secondly, establishing a double-antibody sandwich ELISA (Enzyme-Linked Immunosorbent Assay) method by taking the two monoclonal antibodies as a coating antibody and a detection antibody respectively, and applying the double-antibody sandwich ELISA method to PEDV (Porcine Epidemic Diarrhea Virus) vaccine S protein quantification; the method can be used for detecting semi-finished products and finished products, can be used for process establishment, optimization, vaccine quality evaluation and stability monitoring of porcine epidemic diarrhea virus vaccine antigens, has the advantages of high sensitivity, high accuracy, high repeatability and high flux, is high in detection flux and high in speed, solves the problem that TCID50 cannot meet vaccine quality evaluation at present, and has a good application prospect. The defect that the existing S protein ELISA method can only be used for qualitative judgment is overcome. The monoclonal antibody can also be used for blocking ELISA, capturing ELISA, indirect ELISA, competitive ELISA and a reagent for detecting the porcine epidemic diarrhea virus through a colloidal gold test strip.

The invention provides a hybridoma cell strain secreting a grass carp reovirus VP4 protein monoclonal antibody as well as the monoclonal antibody and application thereof, and belongs to the technical field of aquatic reovirus detection. The hybridoma cell strain is named as GCRV-VP4-6E7B12, and is preserved in the China Center for Type Culture Collection on December 10, 2024, and the preservation number is CCTCC (China Center for Type Culture Collection) NO: C2024160. The grass carp reovirus VP4 protein monoclonal antibody 6E7B12 secreted by the hybridoma cell strain GCRV-VP4-6E7B12 has the advantage of being specifically combined with an antigen. The enzyme-labeled monoclonal antibody is used as a detection antibody, the VP4 protein polyclonal antibody is used as a capture antibody, the content of the grass carp reovirus VP4 protein in a sample to be detected can be quantitatively detected by adopting a double-antibody sandwich ELISA method, the detection range is 1-32 ng / mL, and the lower detection limit is 1 ng / mL. The detection method has the advantages of high sensitivity and wide detection range.

The invention belongs to the field of immunological detection, and provides an anti-human albumin antibody 7G7, an antibody composition and application thereof. The anti-human albumin antibody provided by the invention can be specifically combined with human albumin and has relatively high stability. Based on the antibody, a kit capable of accurately, rapidly and massively detecting the content of HSA in a sample is developed. The double-antibody sandwich ELISA method for detecting the human albumin has high sensitivity, specificity and accuracy, can realize accurate and high-throughput detection of the content of albumin in human serum or other serum-like species containing the human albumin, and has a good application prospect in detection of the human albumin.

The invention discloses preparation and application of a novel diagnostic kit for a mitochondrial network regeneration function evaluation test, and in the invention, combined detection of related proteins DRP1, MFN2, OPA1 and TIM23 is carried out by utilizing four mitochondrial networks, so that the mitochondrial regeneration function state of sample cells is judged. The kit disclosed by the invention is used for detecting samples such as human blood, urine, cell cultures and tissues by utilizing an ELISA (Enzyme-Linked Immunosorbent Assay) method. Results show that the kit can accurately detect the content of four proteins so as to judge the function of sample mitochondria. The mitochondrial network regeneration capacity function evaluation kit based on a double-antibody sandwich method in an ELISA method can conveniently and accurately evaluate the mitochondrial network regeneration capacity in a human body specimen. The invention discloses preparation and application of the novel diagnostic kit for evaluating the regeneration function of the mitochondrial network. The diagnostic kit plays an auxiliary role in mitochondrial detection in scientific research and disease diagnosis and treatment. The method is applied to mitochondrial function evaluation in all diseases with abnormal cell mitochondrial functions.

The invention relates to the technical field of livestock immunology, in particular to a mouse anti-bovine IgG monoclonal antibody and application thereof. The mouse anti-bovine IgG monoclonal antibody provided by the invention can be specifically combined with bovine IgG, has no cross reaction with other similar proteins, has relatively high affinity with bovine IgG, and has relatively high stability; based on the antibody, a double-antibody sandwich ELISA method for detecting bovine IgG has high sensitivity, specificity and accuracy, accurate and high-throughput detection of the IgG content in bovine serum or other serum analogues or products containing bovine IgG can be realized, and the antibody has a good application prospect in bovine IgG detection.

The invention discloses a monoclonal antibody, a hybridoma cell secreting the monoclonal antibody and application of the hybridoma cell, and belongs to the field of peptides. The invention aims to provide a monoclonal antibody and a hybridoma cell secreting the monoclonal antibody. The hybridoma cell secreting the monoclonal antibody disclosed by the invention is a hybridoma cell 3G3, and the preservation number of the hybridoma cell 3G3 in the China General Microbiological Culture Collection Center (CGMCC) is CGMCC No.46345. The monoclonal antibody and the BVDV antibody detection kit based on the monoclonal antibody and the blocking ELISA method can be used for detecting a neutralizing antibody of the BVDV, and have the characteristics of strong specificity, good sensitivity, high sensitivity, high accuracy and the like.

The invention belongs to the technical field of biological detection, and provides a multiple cell factor detection kit in tumor immunity and a preparation method and application thereof, and the detection kit comprises a capture antibody, a detection antibody, a mixed protein standard substance, a diluent and a washing buffer solution. The capture antibody is prepared by coupling a biotinylated antibody with a fluorescent microsphere coated with biotin to obtain a capture antibody coupled with a fluorescent encoding microsphere, and the detection antibody is prepared by coupling derivatized phycoerythrin PE-SMCC with a specific thioether bond of a sulfhydrylated antibody to obtain a fluorescent labeled antibody; the mixed protein standard substance adopts a freeze-drying protection system containing BSA, trehalose and the like. The single hole of the kit can synchronously detect various cell factors, the sample dosage is only 25 microliters, the kit is suitable for immune monitoring of iron overload and other chronic inflammation models, compared with a traditional ELISA method, the efficiency is improved by 10 times, the cost is reduced, and the kit has the remarkable advantages of being high in throughput, wide in linear range and high in stability.

The invention relates to the technical field of biochemical detection, in particular to an enzyme linked immunosorbent assay kit for detecting diisononyl phthalate and application of the enzyme linked immunosorbent assay kit. The invention relates to an enzyme linked immunosorbent assay kit for detecting diisononyl phthalate. The enzyme linked immunosorbent assay kit comprises the following components: an elisa plate coated with a coating antigen, a diisononyl phthalate specific antibody working solution, an enzyme marker working solution, a diisononyl phthalate standard substance solution, a substrate developing solution, a stopping solution, a washing solution, a sample diluting solution and a purifying agent. According to the invention, the content of diisononyl phthalate in an edible oil sample is qualitatively or quantitatively detected by mainly adopting an indirect competitive ELISA method; the sample pretreatment requirement is low, the sample pretreatment process is simple, and a large batch of edible oil samples can be rapidly detected at the same time; the main reagent is provided in the form of a working solution, and the detection method is convenient and easy to implement and has the characteristics of high specificity, high sensitivity, high precision, high accuracy and the like.

The invention belongs to the technical field of biological medicine, and particularly relates to a method for detecting extracellular vesicle protein and application thereof. According to the invention, the design is optimized on the basis of the first-generation SENSORS technology, and a second-generation BarFllare high-sensitivity detection technology is developed. According to the technology, a gold nanoparticle-mediated three-function fluorescent probe controllable release system is integrated, a superparamagnetic nanoparticle-mediated fluorescent probe captures enrichment and enhances a fluorescent signal through a spherical hot spot aggregation effect, the detection sensitivity is improved by 31 times compared with that of a traditional ELISA method, and high-sensitivity detection (1.37-7.30 parts / mu L) of EVs protein is achieved. Compared with the first-generation SENSORS technology, the BarFllare detection time is shortened by 43.75%, and the cost is reduced by about 63%.

The invention discloses a porcine epidemic diarrhea virus S protein monoclonal antibody and application thereof. Specifically, two monoclonal antibodies GC42G2-1 and GC33D8-1 which have broad-spectrum binding characteristics with the porcine epidemic diarrhea virus S protein are obtained firstly, the GC42G2-1 antibody has good universality and can have strong affinity with different strains, and the GC33D8-1 antibody can be used for binding the porcine epidemic diarrhea virus S protein in a broad-spectrum manner. The GC42G2-1 can be independently used, and also can be matched with a polyclonal antibody or GC33D8-1 to be used for sandwich ELISA (enzyme-linked immunosorbent assay) so as to detect and quantify PEDV (porcine epidemic diarrhea virus). Secondly, the two monoclonal antibodies are respectively used as a coating antibody and a detection antibody, a double-antibody sandwich ELISA method is established, and the double-antibody sandwich ELISA method is applied to PEDV vaccine S protein quantification, has better parallelism compared with other antibodies, and can be widely applied to detection of different strains. The method disclosed by the invention has the advantages of high sensitivity, high accuracy, high repeatability and high flux, and can be used for the development of blocking ELISA (Enzyme-Linked Immunosorbent Assay), capturing ELISA, indirect ELISA, competitive ELISA and detection of porcine epidemic diarrhea viruses by colloidal gold test strips.

The invention belongs to the technical field of detection, and particularly relates to a universal kit for bovine nodular skin viruses, sheep pox viruses and goat pox viruses. On the basis of expressed specific recombinant p122 protein and serum to be detected as a detection sample, the double-antigen sandwich ELISA method for the bovine nodular skin virus, the sheep pox virus and the goat pox virus is established, and the method comprises the following steps: coating an antigen, sealing, adding the sample to be detected and an enzyme-labeled antigen, developing, judging a result and the like. The kit has the advantages of being good in specificity, high in sensitivity, good in repeatability, low in cost and capable of achieving batch detection, and a certain detection technology foundation can be laid for preventing and controlling the bovine nodular skin virus, the sheep pox virus and the goat pox virus.

The invention belongs to the technical field of biological medicine, and particularly relates to a nanometer antibody aiming at feline infectious peritonitis virus and application of the nanometer antibody. According to the invention, specific nano antibodies 2S-29 and 2S-39 of two novel FIPV S proteins are obtained by screening through a phage display technology, and a double-antibody sandwich ELISA method for detecting FIPV S antigens is provided. The two antibodies have excellent specificity and affinity to the feline infectious peritonitis virus FIPV S protein. A double-antibody sandwich ELISA method established based on the nano antibody has good specificity, repeatability and sensitivity, the lowest detection limit reaches 4 ng / mL, the FIPV S antigen can be rapidly and accurately detected, and a reliable means is provided for early diagnosis of feline infectious peritonitis.

The invention belongs to the technical field of biological detection, and provides a multiple cell factor detection kit in macrophage polarization and a preparation method and application thereof, and the detection kit comprises a capture antibody, a detection antibody, a mixed protein standard substance, a diluent and a washing buffer solution. The capture system is used for preparing a capture antibody coupled with a fluorescence coding microsphere by coupling a biotinylated antibody with a fluorescence microsphere coated with biotin, and the detection system is used for preparing a fluorescence labeled antibody by coupling derivatized phycoerythrin PE-SMCC with a specific thioether bond of a sulfhydrylated antibody. The mixed protein standard substance adopts a freeze-drying protection system containing BSA, trehalose and the like. The single hole of the kit can synchronously detect multiple cell factors, the sample dosage is only 25 microliters, the kit is suitable for in-vitro tests of mouse mononuclear macrophage leukemia cell lines RAW264.7, compared with a traditional ELISA method, the efficiency is improved by 10 times, the cost is reduced, and the kit has the remarkable advantages of being high in throughput, wide in linear range and high in stability.

The invention relates to detection of vaccine immune effects, in particular to a Marek's disease virus specificity IFN-gamma sandwich ELISA method and application thereof. The invention provides a Marek's disease virus specific T cell immune stimulator, and the amino acid sequence of the Marek's disease virus specific T cell immune stimulator is shown as SEQ ID NO: 1 or SEQ ID NO: 3. The invention further provides a method for detecting the cellular immune level after Marek's disease vaccine immunization. The Marek's disease virus specific T cell immunostimulant can effectively stimulate cell-mediated immune response, initiate and activate MDV specific T cells, so that the Marek's disease virus specific T cells generate Marek's disease virus specific IFN-gamma, and the Marek's disease virus specific T cell immunostimulant has a good application prospect in Marek's disease vaccine immune level detection.

The invention provides a method for establishing a mouse postoperative delirium model under a simulated high altitude urgent entry environment, and relates to the technical field of animal model establishment. According to the method for establishing the model for simulating postoperative delirium of the mouse in the sudden entering plateau environment, a control group (group C), a plateau group (group H), a POD model group (group P) and a plateau POD model group (group HP) are included, behavior changes of the mouse are evaluated through a Y maze experiment, then the mouse is killed, brain tissue is taken, and the mouse is subjected to postoperation delirium simulation in the sudden entering plateau environment. The mouse hippocampus tissue structure damage change is observed through morphological methods such as Nissl staining and a transmission electron microscope, meanwhile, blood is taken through mouse eye sockets, the concentration of inflammatory related indexes IL-6 and TNF-alpha in serum is measured through an ELISA method, and the stability and repeatability of model establishment are known. The plateau postoperative delirium mouse model prepared by the invention can generate obvious damage to brain tissues and neurological functions, and is high in operability and good in repeatability.

The invention provides a human metapneumovirus monoclonal antibody and application thereof, and belongs to the technical field of immunity. The monoclonal antibody F720-1 provided by the invention only has binding activity to conformation F protein before human metapneumovirus fusion, and the monoclonal antibody F390-6 has binding activity to conformation F protein before human metapneumovirus fusion and conformation F protein after human metapneumovirus fusion. Through pairing, it is determined that F720-1 and F390-6 can conduct proportion detection on Pre-F and Post-F in the human metapneumovirus F protein through an enzyme-linked immunosorbent assay double-antibody sandwich method and conduct quantification on Pre-F. The double-antibody sandwich ELISA method has a good linear relation and detection limit when used for detecting the proportion of the Pre-F protein to the Post-F protein in the F protein. The method is expected to be used for accurate evaluation in human metapneumovirus protein experimental vaccine stock solution and vaccine verification.

The invention relates to a method for establishing a PC12 cell model for evaluating the soothing effect of cosmetics. The method comprises the following steps: S1, determining model stimulation conditions; S11, detecting cell viability of a control group and cell viability of capsaicin groups with different concentrations by using a CCK-8 method; s12, observing the activation condition of the capsaicin on the TRPV1 channel of the PC12 cell by using a fluorescence microscope; s13, detecting the expression condition of the capsaicin to the TRPV1 of the PC12 cell by using an ELISA method; s14, detecting the expression condition of the capsaicin on the PC12 cell neuropeptide P substance by using an ELISA (enzyme-linked immuno sorbent assay) method; and S15, detecting the expression condition of capsaicin on IL-33 related to PC12 cell neuroinflammation by using an ELISA method. The invention also relates to evaluating the soothing efficacy of the cosmetic raw material. According to the method, the nerve-like cells are adopted to establish the cell model, the process that human skin is subjected to external stimulation and nerve reaction is excessive is effectively simulated, and the established in-vitro cell model can be used for evaluating the effectiveness of cosmetic raw materials / finished products in protecting or repairing skin nerve disorder and evaluating whether the cosmetic raw materials / finished products have soothing efficacy or not.

This invention provides a double-antibody sandwich SA-ELISA detection kit for Fasciola hepatica infection in sheep. A double-antibody sandwich SA-ELISA method for detecting Fasciola hepatica CAg (cag) is established using mouse anti-Fasciola hepatica D38 monoclonal antibody and rabbit anti-Fasciola hepatica D38 polyclonal antibody. This detection uses monoclonal antibodies as coating antibodies, resulting in high specificity. Furthermore, the introduction of a biotin-streptavidin signal amplification system enhances sensitivity. It offers advantages such as high accuracy, good stability, simple operation, rapid detection, and easy interpretation, making it suitable for rapid clinical diagnosis and epidemiological surveys at the grassroots level.