81 results about "Elisa method" patented technology

The invention relates to the technical field of molecular biology, in particular to an avian adenovirus serum type 4 colloidal gold test strip based on a monoclonal antibody and application of the test strip. According to the invention, two monoclonal antibodies (6B3 and 8G11) aiming at FAdV4 penton protein are successfully prepared through a hybridoma technology, and both the monoclonal antibodies show high titer and strong serotype specificity. Specific analysis proves that the two monoclonal antibodies can recognize 12 FAdV4 isolates from different sources, and do not have cross reaction with other FAdV serotypes or common poultry pathogens. According to the invention, through systematic optimization of key parameters, the detection sensitivity of the test strip is 7.81 * 10 < 4 > TCID50 / 0.1 mL, and the detection time is only 15 minutes. Clinical sample verification shows that compared with an ELISA method, the total coincidence rate is 98.6%.

The invention belongs to the technical field of biochemical engineering, and particularly relates to an amitriptyline hapten and a preparation method thereof, an amitriptyline antigen and a preparation method thereof, an antibody, a kit and application. The amitriptyline hapten has a structural formula shown as a formula (I) or a formula (II). Compared with an existing structure, the amitriptyline hapten prepared by the invention has a better immune effect. The obtained antibody is high in titer, strong in specificity and high in affinity, the LOD of amitriptyline by using an Elisa method established by the antibody is 0.013 ng / mL, the IC50 of amitriptyline is 0.865 ng / mL, the quantitative detection range is 0.07-470.3 ng / mL, the detection sensitivity is high, and the linear range is wide.

This invention belongs to the field of biodetection technology, specifically relating to a monoclonal antibody 1D3 for detecting porcine epidemic diarrhea virus (PEDV) and its applications. Its heavy chain variable region CDR sequences are SEQ ID NO. 1-3, and its light chain variable region CDR sequences are SEQ ID NO. 4-6. This antibody specifically recognizes the PEDV / S1 protein, and its binding can be competitively blocked by PEDV-specific neutralizing antibodies in serum, making it suitable for establishing a blocking ELISA method. Detection tools based on this antibody exhibit high specificity and sensitivity, with blocking rates exceeding 50% against PEDV-positive serum. This invention can be used to prepare detection kits or evaluate vaccine immunization efficacy, providing an efficient and accurate technical means for PEDV infection diagnosis and immune monitoring.

The invention belongs to the technical field of biological detection, and provides a multiple cell factor detection kit in tumor immunity and a preparation method and application thereof, and the detection kit comprises a capture antibody, a detection antibody, a mixed protein standard substance, a diluent and a washing buffer solution. The capture antibody is prepared by coupling a biotinylated antibody with a fluorescent microsphere coated with biotin to obtain a capture antibody coupled with a fluorescent encoding microsphere, and the detection antibody is prepared by coupling derivatized phycoerythrin PE-SMCC with a specific thioether bond of a sulfhydrylated antibody to obtain a fluorescent labeled antibody; the mixed protein standard substance adopts a freeze-drying protection system containing BSA, trehalose and the like. The single hole of the kit can synchronously detect various cell factors, the sample dosage is only 25 microliters, the kit is suitable for immune monitoring of iron overload and other chronic inflammation models, compared with a traditional ELISA method, the efficiency is improved by 10 times, the cost is reduced, and the kit has the remarkable advantages of being high in throughput, wide in linear range and high in stability.

The invention belongs to the technical field of biological detection, and provides a multiple cell factor detection kit in macrophage polarization and a preparation method and application thereof, and the detection kit comprises a capture antibody, a detection antibody, a mixed protein standard substance, a diluent and a washing buffer solution. The capture system is used for preparing a capture antibody coupled with a fluorescence coding microsphere by coupling a biotinylated antibody with a fluorescence microsphere coated with biotin, and the detection system is used for preparing a fluorescence labeled antibody by coupling derivatized phycoerythrin PE-SMCC with a specific thioether bond of a sulfhydrylated antibody. The mixed protein standard substance adopts a freeze-drying protection system containing BSA, trehalose and the like. The single hole of the kit can synchronously detect multiple cell factors, the sample dosage is only 25 microliters, the kit is suitable for in-vitro tests of mouse mononuclear macrophage leukemia cell lines RAW264.7, compared with a traditional ELISA method, the efficiency is improved by 10 times, the cost is reduced, and the kit has the remarkable advantages of being high in throughput, wide in linear range and high in stability.

This invention provides a double-antibody sandwich SA-ELISA detection kit for Fasciola hepatica infection in sheep. A double-antibody sandwich SA-ELISA method for detecting Fasciola hepatica CAg (cag) is established using mouse anti-Fasciola hepatica D38 monoclonal antibody and rabbit anti-Fasciola hepatica D38 polyclonal antibody. This detection uses monoclonal antibodies as coating antibodies, resulting in high specificity. Furthermore, the introduction of a biotin-streptavidin signal amplification system enhances sensitivity. It offers advantages such as high accuracy, good stability, simple operation, rapid detection, and easy interpretation, making it suitable for rapid clinical diagnosis and epidemiological surveys at the grassroots level.

The application belongs to the technical field of biology and particularly relates to an ELISA kit for detecting chicken astrovirus type II antibodies and a preparation method. The Cell-ELISA experiment is carried out by using LMH cells infected with chicken astrovirus type II to detect the chicken astrovirus type II antibodies. The method has no cross reactivity with positive serum of Newcastle disease virus, avian influenza virus, chicken infectious anemia virus, avian leukosis virus, Marek's virus and chicken infectious bursal virus, which indicates that the method has good specificity. The Cell-ELISA method is used to detect the chicken serum sample to be detected by using whole virus infection, and is assembled into a kit for measuring chicken astrovirus antibodies in serum. The kit can be used for serological diagnosis of chicken astrovirus type II infection, monitoring of the level of antibodies, epidemiological investigation and the like, and provides a novel and effective detection means for prevention and treatment of chicken astrovirus type II.

This invention belongs to the field of biotechnology and relates to a specific monoclonal antibody against porcine circovirus type 2 (PCV2) Cap protein and its applications. The CDR of the heavy chain variable region of the monoclonal antibody includes CDR1, CDR2, and CDR3 as shown in SEQ ID No. 1, SEQ ID No. 2, and SEQ ID No. 3, respectively; the CDR of the light chain variable region of the monoclonal antibody includes CDR1, CDR2, and CDR3 as shown in SEQ ID No. 4, SEQ ID No. 5, and SEQ ID No. 6, respectively. A sandwich ELISA method for protein content determination has been established, which is simple to operate, has good sensitivity and specificity, is widely applicable, and can perform high-throughput determination of multiple samples to detect the content of Cap protein, an effective antigen of various types of PCV2 vaccines, thus shortening the vaccine testing time.

The invention discloses a chemiluminescence immunoassay method and kit for detecting progestational hormone of pandas, and belongs to the technical field of biological detection. The specific process comprises the following steps: by taking goat anti-mouse IgG as a coating antibody, enabling a progestational hormone standard substance or a to-be-detected sample and horse radish peroxidase labeled progestational hormone to compete with a limited amount of progestational hormone antibodies together; after incubation and washing, a chemiluminescent substrate is added, and quantitative analysis of the progestational hormone in the panda urine or excrement is realized by detecting the intensity of a luminescent signal according to a standard curve. The invention provides a novel chemiluminescence immunoassay method, the sensitivity of the chemiluminescence immunoassay method is far higher than that of a traditional ELISA method (the lowest detection line can reach 31.25 pg / mL), the operation is simple and convenient, the detection is rapid, and the urgent demand on rapid and accurate determination of the hormone level in panda pregnancy monitoring and reproduction management can be met.

The invention relates to a nano antibody for specifically recognizing a tacrolimus-antibody compound and application of the nano antibody, and belongs to the technical field of biological detection. A variable region of the nano antibody has three complementary determining regions CDR1, CDR2 and CDR3, can specifically recognize a tacrolimus-antibody compound, and can be used for detecting drugs by a double-antibody sandwich method; the kit is widely suitable for various immunodetection technologies such as an enzyme-linked immunosorbent assay (ELISA), a chemiluminescence immunoassay (CLIA), an electrochemical luminescence immunoassay (ECLIA), a fluorescence immunoassay (FIA), an immunochromatography test strip method and the like. According to the method, the limitation of a traditional competition method is overcome, high-sensitivity, high-specificity and high-stability detection is realized, the method can be adapted to various detection platforms, and a portable pretreatment process without centrifugation can also be realized.

This invention relates to a homogeneous photocatalytic chemiluminescence detection system for detecting HPV vaccine antigen content, its preparation method, and its application. The system utilizes self-made carboxyl-modified donor microspheres and receptor microspheres, coupled with HPV type-specific monoclonal antibodies via EDC / NHS activation, achieving one-step homogeneous detection. By optimizing the microsphere size, a 200 nm microsphere system was found to exhibit higher luminescence signal, a wider detection range, and better sensitivity when detecting HPV16 / 18 VLPs. This method is simple to operate, requires no plate washing, and possesses good specificity, accuracy, and precision. It is suitable for the in vitro relative potency and identification detection of bivalent HPV vaccines, and can replace traditional ELISA methods, reducing detection costs and safety risks. It is also suitable for large-scale applications in vaccine quality control.

The application belongs to the field of immunological detection, and provides a double-antibody sandwich ELISA kit for detecting human Cyfra21-1 content. The anti-human Cyfra21-1 monoclonal antibody provided by the application can specifically bind to human Cyfra21-1, has no cross reaction with other similar proteins, and has high stability; based on the antibody, the double-antibody sandwich ELISA method for detecting human Cyfra21-1 has high sensitivity, specificity and accuracy, and can accurately and high-throughput detect the content of Cyfra21-1 in human serum or other serum analogs containing human Cyfra21-1.

The invention belongs to the technical field of biological medicine, and particularly relates to a detection kit for detecting new coronavirus. According to the invention, a new coronavirus NP protein is taken as an immunogen, monoclonal antibodies aiming at specific epitopes of the new coronavirus NP protein are screened, a specific strain is taken as a capture antibody, the other strain is labeled by horse radish peroxidase and taken as a detection antibody, and a double-antibody sandwich ELISA method for detecting the SARS-CoV-2 NP protein is established. Compared with the prior art, the method has the advantages that the detection time is greatly shortened, the detection can be completed in only 6 hours, and a foundation is laid for rapid detection of an SARS-CoV-2 clinical sample.

The invention discloses a colon cancer screening method and kit based on the lactic acid level of a DNA polymerase gamma (Polg) K947 site, and relates to the technical field of biological medicines. Immunohistochemical and serological experiments prove that the lactylation level of the Polg K947 is remarkably reduced in colon cancer patients, and the application value of the Polg K947 in colon cancer diagnosis is disclosed. The application specifically comprises the following steps: detecting the modification level in serum by adopting a specific recognition Polg K947 lactylated antibody through an enzyme-linked immunosorbent assay, taking the modification level as a biomarker for early screening and risk assessment of colon cancer, and providing a corresponding detection kit. The Polg K947 lactylation marker has high sensitivity and specificity, provides a new technical path for non-invasive colon cancer screening, and has a good clinical transformation prospect.

The application provides a screening method of human amniotic membrane mesenchymal stem cells (hAMSCs) with bone regeneration promoting potential, and uses the protein level of transmembrane protein 119 (TMEM119) as a functional index of the bone regeneration promoting ability of the hAMSCs, and uses an enzyme-linked immunosorbent assay (ELISA) to detect the TMEM119 protein concentration in a lysis solution prepared from 1×10 7 The application uses the TMEM119 protein concentration not lower than 15000 pg / mL as a screening standard of the hAMSCs with bone regeneration promoting potential, and screens the hAMSCs with significant bone regeneration promoting potential from different batches of hAMSCs, so that the hAMSCs meeting the screening standard of the application have obvious bone formation promoting potential, and the technical problem that the hAMSCs with bone regeneration promoting potential are difficult to be accurately identified and screened in clinical transformation is solved.

This invention belongs to the field of biodetection technology, specifically relating to a material for detecting anti-Caspr1 antibodies and its preparation method. The invention constructs an expression plasmid capable of highly expressing the Caspr1 antigen, transfects it into HEK293T cells, and modifies the original antigen sequence during construction to achieve normal membrane expression. Subsequently, a modified cell culture protocol is used to obtain a material for detecting anti-Caspr1 antibodies based on cellular immunofluorescence assay. This invention, based on cellular immunofluorescence, avoids the limitations of flow cytometry, which is complex and requires expensive equipment. By modifying the protein expression mode to normal membrane expression, it avoids cell permeabilization, avoids false negatives caused by antigen conformational changes in enzyme-linked immunosorbent assay (ELISA) and Western blotting, and avoids false negatives caused by transmembrane expression due to transfection with the original Caspr1 sequence.

The application discloses a hybridoma cell strain secreting monoclonal antibody PVY-5, the monoclonal antibody and application, the hybridoma cell strain 4B4D8C3 can secrete anti-potato Y virus monoclonal antibody, the preservation number of the hybridoma cell strain is CCTCC NO: C2022282, the monoclonal antibody PVY-5 has specific immunoreaction with the coat protein of potato Y virus, the heavy chain variable region amino acid sequence of the monoclonal antibody PVY-5 is as shown in SEQ ID NO.6, the light chain variable region amino acid sequence is as shown in SEQ ID NO.7, the sensitivity reaches 1:5120 times dilution by using direct ELISA method detection to the leaf of potato Y virus disease infection, the monoclonal antibody PVY-5 can also be used to prepare ELISA kit, or colloidal gold kit, or nano analog enzyme kit, and the potato Y virus disease can be detected quickly, sensitively, economically and accurately.

The invention belongs to the field of biology, and discloses a PDCoV recombinant RBD protein and an IgA Elisa kit thereof, and the amino acid sequence of the recombinant RBD protein is as shown in SEQ ID NO. 1. The recombinant RBD protein is used for an indirect ELISA kit, can cover IgA response in the early stage of infection, can accurately recognize a PDCoV specific IgA antibody in a breast milk sample, and cannot cause false positive due to infection of other common pathogens in a pig herd; the indirect ELISA kit is good in stability (the intra-batch variation coefficient is 1.848%-6.062%, and the inter-batch variation coefficient is 4.504%-7.790%), the coincidence rate of the indirect ELISA kit and an immunofluorescence experiment reaches 93.26%, the repeatability and the coincidence rate are high, and the indirect ELISA kit has the advantages of simplicity and convenience in operation, short time consumption and large detection flux of an ELISA method, and is suitable for large-scale screening of clinical pig milk samples.

This invention relates to a detection kit, detection method, and application for the in vitro relative potency of respiratory syncytial virus (RSV) vaccines; it includes an RSV vaccine reference standard, and Ab101 antibody and Ab3 monoclonal antibody-HRP for detection by a double-antibody sandwich ELISA method. The Ab101 antibody comprises: a heavy chain variable region containing the amino acid sequence shown in SEQ ID NO:1; and a light chain variable region containing the amino acid sequence shown in SEQ ID NO:2. This invention establishes a sensitive, accurate, and specific in vitro relative potency detection system for RSV Pre-F antigen, providing technical support for vaccine quality testing.

The application discloses a kind of based on nano antibody-monoclonal sandwich detection novel coronavirus receptor binding domain protein homogeneous method, it is related to antigen detection field.The expression vector of novel coronavirus RBD protein, nano antibody is constructed to carry out protein expression, the pairing performance of nano antibody and monoclonal antibody is identified by ELISA method, nano antibody and monoclonal antibody are coupled to the surface of fluorescent microsphere and magnetic nanometer microsphere respectively, novel coronavirus RBD protein is diluted into different concentrations using PBS buffer solution, is input into the fluorescent and magnetic nano probe of target RBD protein, fluorescence value is determined after being separated by magnetic frame, and the standard curve for detecting novel coronavirus RBD protein is established, and the minimum detection line is 0.09ng / mL.The immunofluorescence homogeneous method constructed by the application is simple in operation, fast in detection speed, less in steps, low in cost, suitable for a variety of scene detection, and lays a foundation for the development of novel coronavirus rapid detection kit.

The present application relates to the field of immunoassay technology, discloses a product for early diagnosis of sepsis and its application, the composition of the product for early diagnosis of sepsis includes detecting SAA1 protein and PDL1 + The product for detecting the content of exosome in sample is based on antigen-antibody specific immune binding to detect SAA1 protein and PDL1 + The product for detecting the content of exosome in sample, the detection method adopted by the detection product is selected from one of fluorescence immunoassay, enzyme-linked immunosorbent assay, colloidal gold immunochromatography, chemiluminescence immunoassay, electrochemical immunoassay and nanopore-based immunosensing detection method, the product for early diagnosis of sepsis improves the accuracy of early diagnosis of sepsis and disease condition evaluation in a double-index cooperative manner, effectively solves the technical bottleneck that the existing sepsis detection method is insufficient in sensitivity, poor in specificity and difficult to meet the requirements of early precise diagnosis and dynamic disease condition evaluation.

The invention belongs to the technical field of detection, and particularly discloses a measurement uncertainty evaluation method for measuring immune factors in serum by an ELISA (enzyme-linked immuno sorbent assay) method. According to the method, ELISA detection is carried out on immune factors (such as IL-1beta) in serum of an experimental animal in contact with a medical instrument according to standards such as GB / T16886.20-2015, system analysis comprises nine types of uncertainty sources such as repeated detection, standard substance preparation and sample dilution, the standard uncertainty of each component is calculated through mathematical methods such as rectangular distribution and a least square method, and the standard uncertainty of each component is calculated according to the standard uncertainty. And synthesizing the standard uncertainty and calculating the expansion uncertainty, and finally outputting a standard detection result. The method comprehensively covers the uncertainty source, is rigorous in computational logic and clear in key quality control factors, provides a scientific and complete uncertainty evaluation scheme for medical instrument immunogenicity evaluation and immune factor determination in other experimental scenes, is beneficial to laboratory quality control and detection result optimization, and has wide practicability.

The application discloses an immune regulation pharmaceutical effect analysis method based on a concentrated pill of psammochloa and astragalus, which comprises the following steps: raw material extraction, animal model construction, multi-dimensional detection and data statistics. The raw materials of psammochloa and astragalus are weighed according to a specific ratio, and then are prepared into extract by ethanol decoction and concentration. After the experimental animals are grouped and administered intragastrically, the individuals are screened by the four oxygen pyrimidine modeling. The immune organs are separated, weighed, and the index is calculated. The contents of various cytokines in serum and liver tissue are detected by the ELISA method. The tissue morphology, lymphocyte proliferation and protein expression are observed by HE staining, MTT method and immunofluorescence method. The expression of key signal pathway proteins is detected by electrophoresis and other steps after the total protein is extracted, and statistical analysis is carried out. The method is systematic and comprehensive, the result is accurate and reliable, and a scientific basis is provided for the immune regulation pharmaceutical effect evaluation of the concentrated pill.

The application discloses a method for relieving obesity cardiomyopathy lipid toxicity by Chemerin and belongs to the technical field of pharmacological research. Mice are selected to establish an obesity mouse model, and are equally divided into a control group, a high-fat diet group, an AAV CMKLR1 virus group, and a high-fat + AAV CMKLR1 virus group. In succession, mouse heart ultrasound, TSE system detection, glucose tolerance test, and insulin tolerance test are carried out, and mouse blood glucose is measured at intervals. Then, tissue protein is extracted for protein immunoblotting, and tissue RNA is extracted for reverse transcription, and then qPCR is used to detect the expression of related genes. After paraffin embedding and slicing of mouse tissues, HE, WGA, immunofluorescence and other pathological staining are carried out. MTBE method is used for lipid extraction and detection of lipid content. ELISA method is used to measure serum Chemerin, and Acyl-RAC method is used to detect protein palmitoylation. Finally, the experimental results are analyzed to draw a conclusion. The present application proves by clinical data and mouse models that Chemerin compensatorily increases in the process of obesity, and Chemerin can relieve cardiac lipid toxicity caused by obesity and protect cardiac function.

The present application relates to the technical field of biological detection, and particularly relates to a system and a method for detecting oxidized low density lipoprotein. The PLA-HCR metallization system provided by the present application constructs an "AND" logic gate based on a proximity effect, realizes direct recognition and signal amplification of oxLDL complete particles in combination with a hybridization chain reaction, and overcomes the limitation in traditional methods that only rely on indirect reflection of lipoprotein particle concentration between single components; the system uses gold-platinum bimetallic nanoparticles to endow oxLDL particles with high-efficiency exogenous peroxidase catalytic activity, and compared with traditional peroxidase, the enzyme catalytic efficiency is significantly improved, and the system has the ability to directly detect undiluted serum samples, avoiding the cumbersome pretreatment process of traditional ELISA methods. The present application has the characteristics of high specificity, wide detection range and simple operation, and has a wide application prospect in early diagnosis, curative effect monitoring and prognosis evaluation of patients with atherosclerotic cardiovascular disease.

The invention belongs to the technical field of biology, and relates to a monoclonal antibody specifically combined with peste des petits ruminants virus N protein and application thereof. The sequence of the monoclonal antibody CDR1-H is as shown in SEQ ID NO: 1; the sequence of the CDR2-H is as shown in SEQ ID NO: 2; the sequence of the CDR3-H is as shown in SEQ ID NO: 3; the sequence of the CDR1-L is as shown in SEQ ID NO: 4; the sequence of the CDR2-L is LMS; the sequence of the CDR3-L is as shown in SEQ ID NO: 5. The monoclonal antibody can be subjected to specific reaction with peste des petits ruminants virus or N protein of the peste des petits ruminants virus; the enzyme-labeled monoclonal antibody is used as a detection antibody, a double-antibody sandwich ELISA method for detecting the peste des petits ruminants virus is established, the method is good in specificity and high in sensitivity, and the coincidence rate with a commercial kit is as high as 93.75%.

The invention belongs to the technical field of plant virus molecular detection, particularly discloses a multiplex RT-PCR method and a kit for detecting important blueberry viruses, and particularly provides a multiplex RT-PCR detection primer for the blueberry viruses, and the sequence of the primer is shown as SEQ ID NO.1-12. The technical scheme provided by the invention is wide in detection range, high in specificity, high in specificity and high in sensitivity. The kit can be used for simultaneously detecting blueberry scorching virus, blueberry shock virus standard strains and variants thereof, tomato fruit ring spot virus, blueberry necrotic mottle virus and apple mosaic virus, solves the problem that the variants and different virus strains cannot be detected by a traditional ELISA method, covers main blueberry viruses and trans-boundary infected key virus types, and is high in specificity and high in sensitivity. The sensitivity is high.

The application discloses a cell lysate of hAMCs cultured in vitro and a determination method of neurotrophic factor content of culture supernatant, wherein the hAMCs are cultured in vitro, when the cells grow to 80%-90% confluence, subculture is carried out, until the cells reach 80% confluence, then centrifugation is carried out to obtain the culture supernatant of hAMCs, PBS buffer is added to the hAMCs from which the supernatant is removed, centrifugal cleaning is carried out, the cells are broken in an ultrasonic cell disrupter to obtain the cell lysate of hAMCs, and finally, the neurotrophic factor content of the culture supernatant and the cell lysate of hAMCs is determined by using an ELISA method.

The application discloses a SjHNF4 polypeptide antibody, a preparation method and application. The preparation method comprises the following steps: downloading a SjHNF4 protein sequence, screening a polypeptide sequence, adding a cysteine at an N terminal, then performing polypeptide synthesis and purification, coupling to obtain a recombinant polypeptide, mixing the coupled polypeptide with Freund's complete adjuvant to obtain a mixture, immunizing a mammal by using the mixture, taking heart blood of the mammal and separating serum to obtain a polypeptide antibody. The polypeptide antibody is detected by using an ELISA method, and then the polypeptide antibody is purified by using a polypeptide affinity chromatographic column. Finally, the binding affinity of the antibody and the antigen is detected by Western Blot and immunofluorescence experiments. The polypeptide antibody obtained by the application has high purity (not less than 90%) and high titer (not less than 512000), and has good purity and immunogenicity, can satisfy various experimental requirements and has commercial value.

The invention relates to the technical field of drug dose screening, in particular to a method for screening the optimal dose of metformin for treating vascular dementia by using an OGD / R-SHSY5Y cell model, which comprises the following steps: constructing the OGD / R-SHSY5Y cell model to simulate the pathological state of vascular dementia; setting a control group, an OGD / R model group and a metformin treatment group with different concentrations, and additionally arranging a pathway inhibitor verification group; the cell proliferation activity is detected through a CCK8 method, the cell apoptosis rate is detected through an Annexin-V-FITC / PI double staining method, expression of apoptosis-related genes and key genes is detected through a qPCR method and a Western blot method, and the inflammatory factor level is detected through an ELISA method. By integrating multi-dimensional detection results, the optimal dosage of the metformin, which not only can significantly improve cell viability and inhibit cell apoptosis, but also can effectively regulate and control key gene expression and inflammatory factor balance, is screened out. Through multi-index collaborative detection and pathway mechanism verification, accurate screening of the optimal dose of the metformin for treating vascular dementia is realized.