40 results about "Hsp70" patented technology

The invention discloses dsRNA of a targeted potato beetle heat shock protein HSP70-KDa3 gene as well as a preparation method and application of the dsRNA of the targeted potato beetle heat shock protein HSP70-KDa3 gene. In order to solve the problems that an existing RNAi target gene is prone to off-target, poor in field stability and the like, the potato beetle heat shock protein gene LdHSP70-KDa3 serves as an RNA interference target for the first time, and a double-stranded RNA molecule shown as SEQ ID NO: 1 and a preparation method for preparing the double-stranded RNA are provided. Feeding experiments prove that the double-stranded RNA can efficiently silence target genes, the survival rate of potato beetle larvae is remarkably reduced, and feeding and growth development of the potato beetle larvae are inhibited. In addition, the invention further provides a biological preparation containing the double-stranded RNA and application of the biological preparation in prevention and treatment of potato beetles. The dsRNA targeting the HSP70-KDa3 gene provided by the invention has the characteristics of high efficiency, specificity, environmental friendliness and the like, and a new technical scheme is provided for green prevention and control of potato beetles.

The invention belongs to the field of molecular marker screening of aquatic animals, and provides a KASP molecular marker for identifying high temperature resistance of procambarus clarkii and application of the KASP molecular marker. According to the procambarus clarkia high-temperature-resistant gene Hsp70-1 and the procambarus clarkia high-temperature-resistant gene Hsp70-2 disclosed by the invention, two SNP (Single Nucleotide Polymorphism) sites of the procambarus clarkia high-temperature-resistant gene Hsp70-1 and the procambarus clarkia high-temperature-resistant gene Hsp70-2 are remarkably related to high-temperature resistance, Hsp70-1-Hap1 haplotypes and Hsp70-2-Hap1 haplotypes are high-temperature-resistant dominant haplotypes, and the application potential of high-temperature-resistant application through combination of the double genes of KSAP markers developed aiming at the loci are good in typing effect, high in specificity, good in stability, convenient to detect, economical and efficient, and can be widely applied to breeding of high-temperature-resistant varieties of the procambarus clarkii. The KASP marker disclosed by the invention can screen out the procambarus clarkii with the SNP haplotype combination with the high-temperature-resistant advantage, and meets the technical requirements of high-temperature-resistant molecule-assisted breeding of the procambarus clarkii.

Disclosed is a novel class of fusion proteins to recruit the cell's innate chaperone machinery, specifically the Hsp70-mediated system, to specifically reduce TDP-43-mediated protein aggregation and associated protein conformational diseases.

The invention discloses an HSP70-targeting chimeric antigen receptor-macrophage and application thereof in treatment of noise-induced hearing loss, an extracellular domain of the HSP70-targeting chimeric antigen receptor comprises an HSP70 specific binding fragment, an intracellular domain comprises an IL-4 signal channel functional element, a signal switch type CAR structure of the HSP70 is formed, and the macrophage can be used for treating noise-induced hearing loss, so that the HSP70-targeting chimeric antigen receptor-macrophage can be used for treating noise-induced hearing loss. The HSP70-targeted chimeric antigen receptor-macrophage can be used for preparing the HSP70-targeted chimeric antigen receptor-macrophage, is used for mediating polarization of the macrophage to an M2 type and exerting an anti-inflammatory function, is proved to be capable of specifically responding to an inner ear inflammation microenvironment, switching an anti-inflammatory phenotype and protecting hair cells and auditory nerves, can be used for treating noise-induced hearing loss, and can be used for preparing the HSP70-targeted chimeric antigen receptor-macrophage. The limitation that traditional hearing-aid equipment supports treatment on symptoms is broken through, and the hearing-aid device has a remarkable application prospect.

This invention relates to a detection kit and preparation method for enhancing the detection efficacy of autoimmune encephalitis (CBA) antibodies. The kit comprises genetically engineered 293T cells stably integrating and co-expressing the eIF2α S52A mutant and HSP70, and transiently transfected with CBA-associated antigens. The preparation method includes constructing a co-expression vector, establishing a stable transfection cell line, stress preconditioning treatment, antigen transfection, and kit assembly. A method for assessing antibody affinity maturation using this kit is also provided. By synergistically modifying detection cells at three levels—translational regulation, protein folding, and anti-apoptosis—the invention significantly increases antigen expression, reduces apoptosis, and improves detection sensitivity and specificity. Furthermore, it integrates antibody affinity assessment into CBA detection for the first time, providing auxiliary information for clinical staging.

A recombinant heat shock protein (hsp70) or domains thereof is derived from filarial worm Setaria digitata or Homo sapiens so as to provide therapeutic and prophylactic anti-viral agents against respiratory viral infections. In that respect, a method of preparation of the recombinant heat shock protein (hsp70) is used. And the recombinant heat shock protein (hsp70) or domains thereof may be used for treatment and / or prophylaxis of one or more symptoms of respiratory viral infections in a subject.

The application discloses a plant coding gene and protein with improved cadmium resistance and promoted biomass and a transgenic strain. The application carries out RNA-seq sequencing on switchgrass under cadmium stress treatment, screens a differential expression gene with the highest up-regulation fold, and genome annotation shows that the gene codes a member of a heat shock protein 70 family (Hsp70), a chaperone BiP (Binding Protein) protein, and the gene is named as PvBiP2. An overexpression vector of the gene is constructed and is transferred into a cadmium-sensitive yeast mutant strain (ycf1), and the growth condition of the transgenic yeast on a cadmium-containing culture medium is obviously better than that of a control group. Overexpression of the PvBiP2 gene in switchgrass is found to significantly improve the cadmium resistance of the transgenic switchgrass and promote the biomass of the switchgrass.

The invention discloses an application of a corn Zm00001eb409950 gene and an encoding protein thereof in improving or regulating corn kernel traits, a breeding method and a product, and relates to an application of the Zm00001eb409950 gene related to the corn kernel traits in the aspect of high-yield corn breeding, and a breeding method for improving the corn kernel traits. In the gene breeding research process, it is found that the corn gene Zm00001eb409950 for coding the heat shock protein HSP70-22 is related to the characters of corn kernels, and the hundred-grain weight and the vitamin E content of the corn kernels can be increased by overexpressing the gene in corn. When the gene is used for high-yield corn breeding, the breeding period is short, the efficiency is high, and the nutrition of corn kernels can be improved while the corn yield can be accurately improved.

The invention provides a method for cultivating high-temperature-resistant barley pests, which comprises the following steps: putting barley pest larvae under a high-temperature condition for intermittent high-temperature domestication treatment: culturing for 24 hours under a proper-temperature condition after 24 hours of high-temperature treatment each time, and the high temperature is 35 DEG C. Experimental results show that after intermittent high-temperature domestication treatment, CTmax and LTmax of the barley pests are remarkably improved, and the relative content of HSP70 generated under the same high-temperature stress is lower than that of undomesticated barley pests. In addition, the heat tolerance generated by temperature domestication has a certain genetic effect. The invention provides a method for effectively improving the heat resistance of the barley pests, and a high-temperature-resistant barley pest variety is cultivated through the method and has important practical application value.

Provided in the present invention are a heterobifunctional compound for targeted degradation of BRD4 based on HSP70, preparation, and use, belonging to the technical fields of pharmaceutical synthesis and chemical engineering. In the present application, an HSP70 inhibitor is selected as a ligand moiety A that binds to HSP70, a BRD4 inhibitor is selected as a ligand moiety B that binds to BRD4, and the ligand A of HSP70 and the ligand B of BRD4 are linked by means of a linking chain, Linker, to obtain a series of bifunctional degradation agents that can degrade BRD4 to different degrees. The obtained degradation agents have the characteristics of high selectivity and strong activity, and can overcome the disadvantages of BRD4 inhibitors, such as poor selectivity for tumor tissues and strong toxic and side effects.

Provided herein is an agent that targets HSP70, such as antibody and chimeric antigen receptor.Provided is a method for treating cancer, comprising administering an effective amount of an agent that targets HSP70 to a patient in need thereof.HSP70-specific antibody can enhance the uptake of HSP70 by antigen-presenting cells. TIFF2023523890000061.tif79128

The application discloses a chimeric antigen receptor-macrophage targeting HSP70 and application thereof in the treatment of noise-induced hearing loss, wherein the extracellular domain of the chimeric antigen receptor targeting HSP70 comprises an HSP70 specific binding fragment, and the intracellular domain comprises an IL-4 signal pathway functional element, forming a'signal switch type' CAR structure targeting HSP70, which can be used for preparing the chimeric antigen receptor-macrophage targeting HSP70 and mediating the polarization of the macrophage to M2 type and exerting the anti-inflammatory function. It has been verified that the chimeric antigen receptor-macrophage targeting HSP70 can specifically respond to the inner ear inflammatory microenvironment, switch the anti-inflammatory phenotype and protect the hair cells and auditory nerves, and can be used for treating the noise-induced hearing loss, breaking through the limitation of the symptomatic support treatment of the traditional hearing aid device and having a significant application prospect.

The invention belongs to the technical field of biological medicines, and provides a heat stress protein HSP70 inhibitor and application thereof in preparation of antitumor drugs. Experiments prove that the heat stress protein HSP70 inhibitor can enhance the replication capacity of oncolytic viruses in tumor cells and enhance the anti-tumor immune activation capacity at the same time; the invention further proves that the oncoVV-shHSP70 can effectively inhibit the expression of the HSP70 protein, so that the survival rate of various cancer cells can be obviously reduced, the effect is obviously superior to that of a control virus oncoVV only lacking the TK gene, and meanwhile, the oncoVV-shHSP70 can promote the infiltration of CD8 < + >-T cells in tumor tissues. The HSP70 inhibitor can be used for preparing an anti-tumor virus drug, particularly, the HSP70 inhibitor and the vaccinia virus are recombined into a recombinant vector, the advantages of the HSP70 inhibitor and the vaccinia virus can be combined, a new target spot is provided for virus treatment of tumors, and the HSP70 inhibitor has a wide clinical application prospect.

The invention provides a chimeric polypeptide for enhancing immunogenicity of a tumor vaccine as well as a preparation method and application of the chimeric polypeptide. The chimeric polypeptide comprises at least two connected immunological enhancement molecular fragments, and the immunological enhancement molecular fragments comprise CD40L or a functional fragment thereof, and one or more selected from a group consisting of GM-CSF or a functional fragment thereof, HMGB1 or a functional fragment thereof, HSP70 or a functional fragment thereof, FLT3L or a functional fragment thereof, and OX40L or a functional fragment thereof. According to the chimeric polypeptide provided by the invention, the mRNA has a good immunological enhancement effect in cells, antigen-specific cellular immune response can be remarkably induced, and the chimeric polypeptide serving as an immunologic adjuvant can be used for constructing an anti-tumor drug (such as an mRNA vaccine) with a better tumor treatment effect.

The disclosure provides in one aspect compounds, and compositions comprising such compounds, that can be used to treat, ameliorate, and / or prevent cancer, especially colorectal cancer (CRC). In certain embodiments, the compounds of the disclosure inhibit HSP70. In other embodiments, the compounds of the disclosure promote or increase immune cell recruitment to a cancer. In yet other embodiments, the compounds of the disclosure promote and / or increase immune cell infiltration in a cancer.

The invention provides an anti-eperythrozoon ovine HSP70 protein polyclonal antibody as well as a preparation method and application thereof, and belongs to the technical field of bioengineering. The polyclonal antibody disclosed by the invention is prepared by immunizing an animal with an immunogen containing 109th-266th amino acids of the eperythrozoon ovine HSP70 protein, and has the characteristics of high titer and strong specificity. The preparation method of the polyclonal antibody comprises the steps of gene synthesis, protein expression and purification, animal immunization, antibody purification and the like. The invention also provides an application of the polyclonal antibody in immunological detection such as Western Blot, ELISA (Enzyme-Linked Immunosorbent Assay) and the like, and a detection kit containing the polyclonal antibody. The polyclonal antibody provides a core reagent for serological diagnosis of sheep eperythrozoonosis, and has important application value.

The present invention relates to the technical field of Microsporidia cryopreservation. Provided is a cryoprotectant for Enterocytozoon hepatopenaei. The cryoprotectant is mainly prepared from glycerol, dimethyl sulfoxide, and propylene glycol, with PBS buffer as a base solution. A volume percentage ratio of the raw materials dimethyl sulfoxide, glycerol, propylene glycol and PBS buffer is 10%:10%:10%:70%. The raw materials of the cryoprotectant are preferred, such that the obtained cryoprotectant achieves the optimal effect in indicators such as the mortality rate of Enterocytozoon hepatopenaei, the infection load in healthy shrimps, and the influence on polar tube protein 2 (EHP-ptp2) and heat shock protein 70 (hsp70). The present invention lays the foundation for achieving indoor preservation of Enterocytozoon hepatopenaei. The present invention solves the technical problem in the prior art that no cryoprotectant or preservation technique suitable for Enterocytozoon hepatopenaei is currently available.

The invention discloses an application of HSP70-5 and a coding gene thereof in regulating and controlling saline-alkaline tolerance of plants. The invention belongs to the technical field of biology, and particularly relates to an application of HSP70-5 and a coding gene thereof in regulating and controlling saline-alkaline tolerance of plants. The protein, the substance for regulating and controlling the expression of the coding gene of the protein or the substance for regulating and controlling the activity or content of the protein can be applied as follows: 1) application in regulating and controlling the saline-alkaline tolerance of plants and / or application in preparing products for regulating and controlling the saline-alkaline tolerance of the plants; (2) application in plant breeding and / or application in preparation of products for plant breeding, and the protein is shown in the sequence 2. By enhancing, improving or up-regulating the activity of the protein HSP70-5 in a target plant or the expression quantity of the coding gene of the protein HSP70-5, the saline-alkaline tolerance of the plant can be reduced, and a basis is provided for saline-alkaline tolerance breeding of crops.

The invention relates to the technical field of cell engineering, and discloses a chronic heat stress model construction method based on chick embryo primary skeletal muscle cells, which comprises the following steps: step 1, separating and purifying cells, and recording as P0 generation; step 2, differentiating after the cells are subcultured to a P2 generation; step 3, constructing a chronic heat stress model: continuously treating the differentiated cells in an incubator at 43 DEG C for 24 hours; 4, model verification, wherein the effectiveness of the model is confirmed by detecting at least one index of lactic dehydrogenase activity, cell apoptosis rate, ROS level, oxidation reduction state and HSP70 / Caspase-3 gene expression quantity. According to the method, a chronic heat stress model based on chick embryo primary skeletal muscle cells is established, and the in-vitro simulation of the chronic heat stress state of the broiler chicken is realized through standardized cell separation, passage differentiation and continuous 24-hour treatment at 43 DEG C.

Many tumors induce and maintain an immunosuppressive tumor microenvironment (TME) that enables tumor to escape host immune system. The present disclosure identifies that cancer cells secrete exosome / microparticle-free, soluble, phosphorylated Hsp70 (pHsp70) (Heat Shock Protein 70 (Hsp70))), which triggers macrophage (M) M2 polarization. It is a further aspect that lipid nanovesicles (NVs) made of dioleoylphosphatidylglycerol (DOPG) and of DOPG complexed with saposin C (SapC) bind to cancer secreted Hsp70, inhibit M differentiation and polarization, and reduce tumor growth. In addition, administration of DOPG-NVs rendered monocytes insensitive to TLR2 (Toll Like Receptor 2) and TLR6 (Toll Like Receptor 6) ligands, suggesting that administration of DOPG-NVs interferes with TLR function.

The invention relates to the technical field of molecular biology and environment, and discloses a primer group, a kit and application thereof, and a method for detecting biotoxicity of an environmental sample, the primer group comprises a primer pair for specific amplification of an hsp70.3 gene and an mt2 gene, and a primer pair for specific amplification of a reference gene; the primer pair for specifically amplifying the hsp70.3 gene comprises an upstream primer with a nucleotide sequence as shown in SEQ ID NO.3 and a downstream primer with a nucleotide sequence as shown in SEQ ID NO.4; the primer pair for specifically amplifying the mt2 gene comprises an upstream primer with a nucleotide sequence as shown in SEQ ID NO.5 and a downstream primer with a nucleotide sequence as shown in SEQ ID NO.6. The primer group disclosed by the invention can be used for monitoring the content change of hsp70.3 and mt2 in a living body, so that the biotoxicity of a to-be-detected environmental sample can be analyzed, the early warning of heavy metal pollutants is realized, particularly the existence of the heavy metal pollutants in the to-be-detected environmental sample can be prompted, and the heavy metal detection can be further combined for analysis in the later period.

In certain aspects, provided herein compositions and methods for treating inflammation or an inflammatory disease using an mRNA formulated in a lipid nanoparticle (LNP), wherein the mRNA comprises an open reading frame encoding HSP70 polypeptide, and methods of using the same.

This invention discloses a molecular marker for heat shock protein 70 in Litopenaeus vannamei, which is associated with cold tolerance. The markers include molecular markers A-F located on the heat shock cognate 70 gene sequence (Sequence 1) and molecular markers G-W located on the hsp70-binding protein 1 gene sequence (Sequence 3). These molecular markers are significantly correlated with cold tolerance in Litopenaeus vannamei and can serve as functional markers for cold tolerance, improving the efficiency of cold-tolerant breeding and allowing for the selection of cold-resistant individuals for subsequent genetic breeding. The heat shock protein 70 molecular markers for cold tolerance in Litopenaeus vannamei are amplified and sequenced using genomic DNA from Litopenaeus vannamei muscle tissue as a template. The genotypes of molecular markers A-W are then identified to screen for individuals with dominant genotypes.

Lysosomal membrane permeabilization is an evolutionarily conserved hallmark of stress-induced cell death. Here the inventors show that the major stress-inducible heat shock protein 70 (Hsp70) enhances cell survival by stabilizing lysosomes through a pH-dependent high affinity binding to an endo-lysosomal anionic phospholipid bis(monoacylglycero)phosphate (BMP; also referred to as lysobisphosphatidic acid). The positively charged ATPase domain of Hsp70 is responsible for the binding but the substrate-binding domain is also required for effective stabilization of lysosomes. Importantly, the cytoprotective effect can be obtained by endocytic delivery of recombinant Hsp70 and specifically reverted by extra cellular administration of BMP antibodies or Hsp70 inhibitors. Thus, this protein-lipid interaction opens exciting possibilities for the development of cytoprotective and cytotoxic lysosome-specific therapies for the treatment of degenerative diseases and cancer, respectively.

A novel class of fusion proteins is disclosed to recruit cellular innate chaperones, particularly the Hsp70-mediated system, to specifically reduce TDP-43-mediated protein aggregation and associated protein conformation disorders.

The invention discloses an engineered immune cell as well as a preparation method and application thereof, the engineered immune cell is transformed through genetic engineering, and (1) a Gasdermin D N-terminal fragment (GSDMD-N) expression cassette controlled by a heat shock protein HSP70 promoter is stably integrated in a genome; (2) a photothermal conversion material and / or magnetic nanoparticles used for exogenous stimulation heating are loaded in the cells; and (3) attenuated bacteria and / or anti-tumor drugs for therapeutic loading; wherein the nucleotide sequence of the HSP70 promoter is as shown in SEQ ID NO: 1, and the nucleotide sequence of the GSDMD-N is as shown in SEQ ID NO: 2. According to the invention, accurate and controllable deployment of anti-cancer drugs such as bacteria loaded in immune cells at tumor sites is realized, good anti-tumor effect and high safety are achieved, and a new technical platform is provided for cell therapy and living microorganism anti-tumor therapy.

The invention discloses a fluorescence and photoacoustic imaging guided dual-targeting nano platform as well as a preparation method and application thereof, and belongs to the technical field of biomedical materials and tumor photothermal therapy. The nano-platform comprises a photosensitive molecule ATTTO regulated and controlled by excited state engineering, a CO release component and a PB nano-carrier, and the three components are cooperatively assembled to form a PB (at) ATTTO-CO composite structure. Under the conditions of near-infrared illumination and oxidative stress, the platform can synergistically release CO and generate a photothermal effect at low temperature, and the expression of heat shock protein HSP70 is remarkably reduced, so that the sensitivity and curative effect of low-temperature photothermal therapy are enhanced. The platform has mitochondria-cell nucleus double subcellular targeting capability, can realize fluorescence / photoacoustic / infrared thermal three-mode imaging navigation, and can be used for precise diagnosis and treatment integration of tumors. The preparation method is simple and controllable in parameters, and has good stability, biocompatibility and industrial application prospect.

This invention provides an active agent that targets HSP70. [Solution] Active agents targeting HSP70, such as antibodies and chimeric antigen receptors, are provided herein. A method for treating cancer is provided, comprising the step of administering an effective amount of an HSP70-targeting active agent to a patient in need. HSP70-specific antibodies can enhance the uptake of HSP70 by antigen-presenting cells.

The application relates to the technical field of biotechnology, and particularly discloses a recombinant NMDA receptor protein and application thereof, wherein the recombinant NMDA receptor protein comprises an amino acid sequence shown in SEQ ID No. 1. The application further provides a cell stably overexpressing the recombinant NMDA receptor protein and applying the cell to preparation of an anti-NMDA receptor antibody encephalitis detection product. The recombinant NMDA receptor protein is constructed by combining a core sequence of HSP70 protein with a complete sequence of the NMDA receptor protein, so that the expression amount of the recombinant NMDA receptor protein is higher, and the binding capacity with the anti-NMDA receptor antibody is stronger. The cell stably overexpressing the recombinant NMDA receptor has a higher binding efficiency with the anti-NMDA receptor antibody, the sensitivity of a detection reaction is further improved, and the detection result is more accurate.