30 results about "Lysosomal membrane" patented technology

A topical skin rejuvenation preparation to relieve wrinkles, increase the skin's natural production of hyaluronic acid, reverse the lack of suppleness, hydrate from within, erase spider veins, reduce varicose veins, lighten aging dark blotches (“liver spots”/Lentigos, Senile Lentigines), decrease acne, and reduce under eye puffiness includes Glucosamine (2-amino-2-deoxy-alpha-D-glucose), a hexosamine (6 carbon amino sugar), including its derivative and precursor compounds: Glucosamine Sulfate, Glucosamine Hydrochloride, Glucose-6-Phosphate, Acetyl Glucosamine, Fructose-6-phosphate, Glucosamine-6-Phosphate, to increase production of Hyaluronic acid and collagen from Glucosamine Sulfate, its precursors and derivatives and to increase skin muscle tone by Dimethylaminoethanol (DMAE) while over coming deficiency it creates in each cell's production of PhosphatidylCholine, whose deficiency damages cell membranes, as well as mitochondrial and lysosome membranes.

The invention relates to the field of accessories and preparations with medicinal functions, in particular to a kind of oligopeptide-based pH-sensitive amphoteric ion lipid (I) or (II). In the invention, the derivatives of the oligopeptide amphoteric ion lipid have different surface charges under different pH conditions and can take relatively strong negative charges in a physiological environment with pH near neutrality so that the lipid has good blood compatibility; when the lipid reaches a tumor part, the surface charges of the oligopeptide amphoteric ion lipid are reversed in an acidulous tumor environment, so that the original electronegativity is reversed into electropositivity; and a carrier with positive charges is easily combined with the surface of a tumor cell, and the tumor cell targeting can be effectively realized through an endocytosis path. In lysosome, a cationic carrier continuously realizes a function of proton sponge or a function of splitting lysosome membranes, and the two functions can ensure the integrity of the carrier, so that the carrier can safely escape to cytoplast or other organelles.

The invention discloses a siRNA-loading nanoparticle. The siRNA is loaded in a duct of mesoporous carbon dioxide through a nonelectrostatic effect, an electropositive high molecule is wrapped on the surface and high molecular polyethylene glycol with biocompatibility and a polypeptide molecule which modifies a transcellular membrane and an lysosome-breaking membrane are connected, so that the nanoparticle has good biocompatibility and the interference effect of the siRNA is improved. The interference effect of the siRNA is remarkably stronger than that of a commercial lipidosome. The nanoparticle has a wide prospect in preparation of cancer gene therapeutic drugs.

This invention relates to a fusion protein that possess action of selectively kill tumour rebirth blood vessel endotheliocyte, and its application. This fusion protein through connecting peptide connect VEGF121 with amido end of sponge gourd seed ribosome inactivating protein(RIP) Luffin Alpha. The vascular endothelial cell growth factor VEGF121 act as means of delivery, make this fusion toxin idiosyncratic incorporate with vascular endothelial cell growth factor receptor F1k1/ KDR, thereby optionally ingress tumour rebirth vascular endothelial cell; the polypeptide possess amphipathic molecule character, by destroy lysosome membrane to promote the release of lysosome inside dissociate toxin molecule; the toxin carboxyl terminal endoplasmic reticulum loacting signal led toxin molecule( sponge gourd seed RIP Luffin Alpha) to target site to exert toxic effect, so to destroy rebirth blood vessel of tumour organization, cut off tumour blood supply, achieve the end of restraining tumor.

The invention discloses a novel nano-drug with a tumor treatment action and a preparation method. The preparation method comprises the steps of carrying adriacin doxorubicin with hollow mesoporous SiO2 nanoparticles (HMSNs), and then treating with bicarbonate to obtain nano-bomb. When drug enters cell lysosome by endocytosis, H<+> in the lysosome reacts with the drug and fast generates CO2 gas; the generated gas damages completeness of the lysosome or improves the permeability of a lysosome membrane, induces the death of cells related to the lysosome, therefore, cancer cells are killed. The nano-bomb also has well treatment effect for drug-resistant strains, and can effectively overcome tolerance of the cancer cells.

The invention discloses a method for preparing defatted marinated duck meat by virtue of ultrasonic-assisted pressure-transform tumbling. The method comprises the following steps: firstly defreezing frozen duck leg meat, and pickling the duck leg meat with a pickling solution containing tea leaves so as to remove reactive oxygen free radicals, intercept the peroxidation process of lipid and increase the pleasant tea fragrance of the defatted marinated duck meat; then processing the duck leg meat by virtue of adopting an ultrasonic-assisted pressure-transform tumbling method, wherein due to the effects of changeable pressure and ultrasonic waves, damage to myofibril structures and lysosomal membranes is promoted, so that the shear force value decrease is more remarkable, more salt soluble protein can be released, therefore more moisture is reserved, the effect of tenderizing and smoothing duck meat is achieved, and furthermore, permeation of a pickling solution is further promoted during a tumbling process; performing baking-based defatting on the duck leg meat, wherein by virtue of reasonable temperature control, while the subcutaneous fat of the duck meat is reduced, the fat and moisture in the duck meat can not be reduced, optimal taste of the duck meat can be kept, and the content of tea polyphenol in the duck meat is not damaged.

The invention provides a method for constructing a chemical biological screening system. The method comprises the following steps: cultivating caenorhabditis elegans by using a 24-pore plate; settingdifferent experimental groups; wrapping the 24-pore plate with tin foil paper to keep away from light and prevent irradiation; putting into a shaking table and performing cultivation; after 48 or 72 hours, putting the cultivated caenorhabditis elegans into a glass slide, putting under a microscope and observing the change of the stable state of a lysosomal membrane in macrophage in the body cavityof the caenorhabditis elegans. The invention further provides a natural micromolecular compound as well as application thereof to preparation of a medicine for promoting lysosome mediated necrocytosis disease, wherein the natural micromolecular compound can promote lysosome mediated necrocytosis and is screened out according to the method for constructing the chemical biological screening system.

The invention relates to a preparation method for a lysosomal membrane coated nanoparticle. Under the function of the driving of macrophages, an active lysosomal membrane coated nanoparticle is synthesized. Specifically, the preparation method comprises the following steps of: firstly, activating the macrophages by lipopolysaccharides, stimulating the secretion of lysosomal related proteases in the macrophages, and enhancing the enzymatic activity of the lysosomal related proteases; then, utilizing the phagocytic ability of the macrophages to jointly incubate the prepared nanoparticle and themacrophages to enable the macrophages to carry out phagocytosis on the nanoparticle, and carrying out internalization on the nanoparticle in macrophage lysosome; and finally, utilizing a lysosome extraction kit to extract the lysosome to obtain the lysosomal membrane coated nanoparticle. Compared with a preparation method for a traditional lipid membrane coated nanoparticle, on one hand, the preparation method disclosed by the invention is simple, and does not need to consider damage caused for the membrane by pressure or electric shock. On the other hand, the macrophages are taken as drivingforce, and the prepared lysosomal membrane coated nanoparticle keeps the activity of hydrolase in the lysosome.

The invention relates to a preparation method of an intelligent nanoparticle capable of specifically accelerating tumor cell apoptosis and monitoring the curative effect by itself. According to the intelligent nanoparticle, poly(D,L-lactide-co-glycolide)-polyethylene glycol, poly(D,L-lactide-co-glycolide)-polyetherimide and distearoyl phosphoethanolamine-polyethylene glycol-folic acid are adopted as a carrier and covered with hydrophobic anticancer drugs and caspase-3 substrate fluorescent peptide through a one-step self-assembling process. The nanoparticlle can specifically recognize tumor cells overexpressed by folate receptors, and enters lysosome through receptor-mediated endocytosis. By means of the proton sponge effect of polyetherimide, a lysosome membrane is through, escape of the drugs from the lysosome is promoted, tumor cell apoptosis is induced, the iconic caspase-3 of cell apoptosis is activated, the substrate fluorescent peptide is cut, fluorescent signals are generated and used for monitoring the curative effect in real time, precise treatment of tumor cells is achieved, and great tumor diagnosis and treatment application prospects are achieved.

The invention relates to a self-assembled nano material and in particular to discloses a polypeptide lipidosome capable of transforming shape in a lysosome of a tumor cell. The main body of the polypeptide is a peptide chain formed by six alanines, a benzene ring-containing radial is modified at the terminal N of the peptide chain, and the terminal C of the peptide chain is connected to an RGD polypeptide sequence. The polypeptide under a weakly physiological pH can be self-assembled to form a lipidosome-like structure and is then recognized by the tumor cell and enters the lysosome by means of endocytosis, and the polypeptide responds to the acidic pH in the lysosome. The self-assembled shape lipidosome structure is transformed to nanofibers to induce permeabilization of a lysosome membrane, so that more cathepsins are released to cytoplasms. When the polypeptide lipidosome coats a drug capable of inhibiting lysosome repair or accelerating lysosome to release the cathepsins, the polypeptide lipidosome has a synergistic effect with polypeptide nanofibers, so that the cytotoxicity is enhanced.

The invention discloses diammonium glycyrrhizinate microemulsion and a preparation process thereof, providing a microemulsion preparation for patients with viral hepatitis. The diammonium glycyrrhizinate microemulsion is prepared from a medical raw material and three auxiliary materials; and the preparation method of diammonium glycyrrhizinate microemulsion comprises a step of preparing auxiliary material solution, a step of preparing raw material solution and a step of preparing microemulsion. The preparation method of diammonium glycyrrhizinate microemulsion is scientific and novel; the preparation process is simple and convenient; the diammonium glycyrrhizinate microemulsion has more functions of non-specific anti-inflammatory, anti-allergy, stable effect on lysosome membrane and immunoregulation in clinical application; in addition, the diammonium glycyrrhizinate microemulsion improves the thermodynamic activity, prevents the first-pass effect of liver and is easier to work. Compared with the other diammonium glycyrrhizinate oral liquid preparations, the diammonium glycyrrhizinate microemulsion has higher bioavailability and more remarkable efficiency of antiviral therapy and is more convenient for patients, who have difficulty to swallow solid medicine.

The invention discloses plant essential oil emulsion for treating poultry respiratory syndromes and a preparation method thereof. With the adoption of an essential oil emulsifier manner, the plant essential oil emulsion disclosed by the invention is capable of greatly improving absorption of poultry on Chinese herbal medicines and improving prevention and treatment effects. Moreover, the medicine is surrounded by an oil phase, the storage reliability is improved, and the medicine is prevented from losing efficacy. According to clinical verifications, the plant essential oil emulsion disclosed by the invention has the effects of inhibiting fusion of viral inclusions or membranes of lysosome, reducing influenza virus replication and resisting respiratory syncytial viruses, has good effects of diminishing inflammation and killing bacteria, relieving cough and asthma and well preventing and treating poultry respiratory diseases and hardly has any toxic or side effect.

The invention discloses compound triamcinolone acetate urea-cream and a preparation method thereof, relating to the technical field of medicine. The compound triamcinolone acetate-urea cream consists of triamcinolone acetate, urea and an emulsion substrate. In the compound triamcinolone acetate-urea cream, triamcinolone acetate serves as a medium-efficiency glucocorticoid which can be used for eliminating fever, flushing and swelling caused by local non-infectious inflammation; and urea has the effects of dissolving and denaturalizing keratoprotein and enhancing the cuticle layer hydration, so that skin is softened, and cracking is prevented. The compound triamcinolone acetate urea-cream has the effects of suppressing movement of inflammatory cells to inflammatory positions, preventing inflammatory media from being released, stability a lysosome membrane, suppressing immune reactions and improving the stress ability of a mechanism to noxious stimulus.

The invention discloses an influenza virosome (VI) coated bionic nano vaccine and a preparation method thereof. The bionic nano vaccine comprises VI, small-particle-size fluorinated particles and DNAvaccines, the bionic nano vaccine is a core-shell structure nano system, the VI is lipid vesicles containing influenza virus envelope protein, inner cores of the nano system are small-particle-size fluorinated particles loaded with the DNA vaccines, and the VI wraps the surfaces of the inner cores. The VI provided by the invention has receptor binding activity, lysosome membrane fusion activity and antigen activity of influenza virus; and the small-particle-size fluorinated inner cores can deliver DNA into cores to promote protein expression. The nano system can realize the common loading of protein vaccines and the DNA vaccines and the site-specific delivery of each component, and finally a synergistic effect is achieved to improve the immune effect. The invention belongs to the field ofpharmaceutical preparations and biological medicines. Influenza virus infection can be effectively prevented, and very high clinical application value is achieved.

The invention relates to a biological preparation for preventing influenza and a preparation method of the biological preparation. The method comprises the following steps: weighing the following raw materials in percentage by weight: 20%-30% of echinacea, 20%-30% of houttuynia cordata, 5%-25% of citron day-lily, 10%-20% of wild chrysanthemum flower and 10%-20% of rhodiola rosea; adding purified water which is 6-10 times of weight of the raw materials, and stirring and mixing the raw materials; carrying out reflux extraction, centrifugally separating an extract liquid, and intercepting the obtained clear liquid in an ultrafiltration membrane to obtain a residual liquid A and dialysate; putting the dialysate into a macroporous resin HPD-100 chromatographic column for chromatography, and eluting with a 30%-80% ethanol solution to obtain an eluant B; mixing the residual liquid A with the eluant B, and carrying out vacuum concentration at 60-80 DEG C to obtain a concentrated liquid; and putting the concentrated liquid into a vacuum drying oven to obtain a dried product, namely the preparation, under the conditions that the temperature is 65-85 DEG C and the vacuum degree is 500-800Pa. Compared with the prior art, the biological preparation has the advantages that the immunity of the organism is enhanced; the cellular and humoral immunity is improved; the influenza virus is directly inactivated; fusion of an endosome/lysosomal membrane is suppressed; and influenza virus replication is reduced.

The invention discloses a method for carrying out lysosome classification by taking a metabolite of the lysosomes as a marker. The method comprises the following steps: obtaining contents of a single lysosome in a cell by adopting a single lysosome patch clamp technology; performing mass spectrometric detection on the lysosome content to obtain the composition and content of metabolites in the lysosome content; and classifying the lysosomes according to a detection result and differences of metabolite composition and content in a single lysosome content to obtain five different subgroups of lysosomes, including known autophagy lysosomes and endocytosis lysosomes. The lysosomes can be classified more finely, the lysosomes can be divided into five types by adopting the method, and each type of lysosomes has an obvious classification marker. Researchers provide clues for studying the specific functions of various lysosomes in the fields of aging and degenerative diseases such as development of drug metabolism for treating Alzheimer's disease and cancer.

The invention relates to the technical field of medicines and in particular relates to a pharmaceutical composition of Siramesine and snake venom cytotoxin-CTX1 and application of the pharmaceutical composition to preparation of anti-tumor drugs. Sira is a sigma2 receptor stimulant; after the Sira is combined with a sigma2 receptor, the permeability of a lysosome membrane is increased and tumor cells are induced to die; the Sira also can improve the pH (Potential of Hydrogen) value of lysosome of the tumor cells so that ROS is raised and program death of the cells is started. The Sira is combined with CTX1 so that the effect of the CTX1 on the lysosome is enhanced; the dosage of the CTX1 in the pharmaceutical composition is only 1/3.6 to 1/2.3 of the single dosage; the composition has a relatively strong cooperative effect and a tumor cell killing effect is remarkably improved; the pharmaceutical composition also has a remarkable killing effect on the drug-resisting tumor cells. The dosage of the drug is relatively low, so that the side effect and the untoward effect rate in a drug utilization process are reduced, the treatment cost is reduced and the economic burden of patients isalleviated.

The invention discloses a nano-composite, a preparation method of the nano-composite and application of the nano-composite. The nano-composite is prepared from cationic polymer carriers, nucleic acid molecules and conjugated polyelectrolyte. The preparation method comprises the following steps of mixing the cationic polymer carriers with the nucleic acid molecules, adding the conjugated polyelectrolyte, and standing for 10 to 60min so as to obtain a multi-component nano-composite solution. The nano-composite is applied to enhancing the transportation of nucleic acid. The nano-composite has the advantages that the surface of the nano-composite is functionally transformed by adopting a non-covalent assembly way, so that the nano-composite is not only strong in stability, but also is applied to the transportation of the nucleic acid, therefore the permeability of a lysosome membrane in an illumination or non-illumination condition can be enhanced, the lysosome escape of the nano-composite is realized, a cell can be prevented from being damaged obviously, the gene delivery efficiency and nucleic acid transportation efficiency are increased, and the requirements of safety and high efficiency on gene delivery are met; meanwhile, the preparation method is reasonably designed, the preparation process is simple, the usage amount of a carrier material is greatly reduced, and a wide application prospect is obtained.

The invention relates to the field of prevention and/or treatment of hepatocellular carcinoma, in particular to application of DC661 in treatment of hepatocellular carcinoma. Through research, the inventor finds that DC6611 can effectively down-regulate expression of a key molecule HSP70.1 in an HSP70.1/BMP/ASM pathway for maintaining lysosome membrane stability and inhibit lysosome, so that autophagy is inhibited, tumor cell apoptosis is induced through a mitochondrial pathway, an anti-tumor effect is achieved, and then the application of DC661 in preparation of drugs for preventing and/or treating individual hepatocellular carcinoma is provided. A new choice is provided for treating hepatocellular carcinoma by adopting a non-surgical method.