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siRNA-loading nanoparticle and application thereof

A nanoparticle and particle technology, applied in the field of biomedicine, can solve the problems of low biocompatibility, blocked functional modification, poor interference effect, etc., and achieve the goal of improving biocompatibility, improving phagocytosis, and improving tolerance Effect

Inactive Publication Date: 2014-09-10
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The particle system initially achieved the interference effect of siRNA at the cellular level, but the effect was minimal
In 2012, Hartonno, S.B. et al. (ACSNano, Volume 6, Page 2104, 2012) synthesized silicon oxide nanoparticles with large pore size (greater than 10nm), and successfully modified positively charged polymer materials on the outer surface of the particles and in the pores Poly-L-lysine PLL (Poly-L-Lysine) adsorbs siRNA through electrostatic interaction to achieve siRNA interference, but the interference effect of the particles is relatively low, and the particles are highly toxic at low concentrations and have poor biocompatibility high
In 2013, Lin, D et al. (Nanoscale, Volume 5, Page 4291, 2013) reported the synthesis of mesoporous silica nanoparticles with a large pore size (10nm), by modifying the electropositive polymer methacrylic di DMAEMA was used to adsorb siRNA, and the interference of the target gene was achieved, but the interference effect was worse than that of commercial liposomes
Since DMAEMA adsorbed a large amount of siRNA in the particle system, the active site of DMAEMA was largely occupied by siRNA, which hindered further functional modification.

Method used

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  • siRNA-loading nanoparticle and application thereof
  • siRNA-loading nanoparticle and application thereof
  • siRNA-loading nanoparticle and application thereof

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Embodiment Construction

[0020] In order to make the present invention easier to understand, the present invention is further described in conjunction with specific examples. The following examples are only used to illustrate the present invention and are not intended to limit the scope of the present invention.

[0021] In the following examples, we selected a kind of mesoporous silica nanoparticles (magnetic mesoporous silica nanoparticles, M-MSNs) with a magnetic core to carry out siRNA loading test, the method and parameters of loading siRNA on mesoporous silica nanoparticles See the Chinese patent application number 201110266192.7 for the conditions. The particle size of the M-MSNs selected in this example is about 50 nm, and the pore size of the mesoporous channels is 3.6 nm.

[0022] 1. Preparation of M-MSN_siRNAPEI

[0023] 1. First add 0.2mg of M-MSNs to a 2mL centrifuge tube, then add 0.042mL of pure ethanol to it, and ultrasonically oscillate for 30s at 100w to disperse the particles even...

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Abstract

The invention discloses a siRNA-loading nanoparticle. The siRNA is loaded in a duct of mesoporous carbon dioxide through a nonelectrostatic effect, an electropositive high molecule is wrapped on the surface and high molecular polyethylene glycol with biocompatibility and a polypeptide molecule which modifies a transcellular membrane and an lysosome-breaking membrane are connected, so that the nanoparticle has good biocompatibility and the interference effect of the siRNA is improved. The interference effect of the siRNA is remarkably stronger than that of a commercial lipidosome. The nanoparticle has a wide prospect in preparation of cancer gene therapeutic drugs.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to a nanoparticle loaded with siRNA and its application in preparing gene medicine for cancer treatment. Background technique [0002] With the advancement of cell and molecular biology technology, RNA interference technology has been widely used in the field of gene therapy for exploring gene function and infectious diseases and malignant tumors (Nat.Rev.Mol.CellBiol.4 volume, 457 pages, 2003 ). At present, the existing RNA interference technology mainly introduces chemically synthesized siRNA into cells, and then recognizes specific messenger RNA to realize the interference function. However, siRNA synthesized in vitro has the disadvantages of being easily degraded and unstable in action time after entering the body. Therefore, the biggest obstacle in the application of RNA interference technology is the lack of a carrier that can stably and effectively induce siRNA into cells. Toxic ...

Claims

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Application Information

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IPC IPC(8): A61K48/00A61K47/42A61K47/04A61P35/00
Inventor 古宏晨陈一杰夏伟梁
Owner SHANGHAI JIAO TONG UNIV
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