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Methods and products for manipulating uncoupling protein expression

a technology of uncoupling protein and expression, which is applied in the direction of liposomal delivery, peptide/protein ingredients, and macromolecule non-active ingredients, etc., can solve the problem that the cell does not always instruct the cell to di

Inactive Publication Date: 2011-08-25
UNIVERSITY OF VERMONT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

The invention is about the discovery of a protein called UCP (uncoupling protein) in the plasma membrane of rapidly dividing cells, such as tumor cells, and its role in regulating cell division. The invention provides methods for inhibiting the expression of plasma membrane UCP to prevent cell division and treat diseases associated with excessive cellular division, such as cancer and autoimmune diseases. The invention also includes the use of plasma membrane targeted UCP inhibitors, such as UCP binding peptides or molecules, anti-UCP antibodies, hydrophobic nucleotide analogs, and UCP antisense. The invention also provides compositions of UCP and UCP inhibitors associated with plasma or lysosomal membrane targeting molecules.

Problems solved by technology

These mediators, however, do not always instruct a cell to die.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

Metabolic State of a Cell is Indicative of Cell Surface Fas Expression and Sensitivity / Resistance to Cell Death

[0233]1. Resistance to apoptosis is characterized by failure to express Fas: The cell lines utilized herein include L1210, a leukemic cell line; HL60, a human pro-myelocytic cell line; and PC12, a pheochromocytoma cell line which can be induced to differentiate into a neuronal cell line in the presence of NGF (Lindenboim, L, et al., Cancer Res, 1995, 55:1242-7). Each cell line was examined in parallel with apoptotic resistant sublines: L1210 DDP, HL60 MDR, and PC12Trk. L1210 DDP are resistant to cisplatin and methotrexate; HL60 MDR are resistant to adriamycin induced apoptosis; PC12 to TrkA, which have been transfected with TrkA which results in constitutively expression the NGF receptors, are not susceptible to alcohol and NGF withdrawal as are the PC12 cells.

[0234]The apoptosis sensitive cells from each tissue origin were morphologically round, non-adherent, rapidly divid...

example 2

Metabolic State of a Cell is Indicative of Cell Surface and Intracellular UCP Expression and Sensitivity / Resistance to Cell Death

[0260]1. Intracellular UCP is Present in a Panel of Tumor Cells.

[0261]We extended our analysis of intracellular expression of UCP to other tumor cells. Intracellular UCP expression was examined flow cytometrically on cells which had been permeabilized and stained as indicated. The histograms represent FITC isotype control versus stained with Rabbit anti-UCP (a kind gift of Mary Ellen Harper) FITC-anti-Rabbit. A Coulter Epics Elite flow cytometer with a single excitation wavelength (488 nm) and band filters for PE (575 nm), FITC (525 nm) and Red613 (613 nm) was used to analyze the stained cells. Each sample population was classified for cell size (forward scatter) and complexity (side scatter), gated on a population of interest and evaluated using 40,000 cells. Criteria for positive staining were established by comparison with isotype controls, thin lines t...

example 3

Exposure of Chemotherapy Sensitive Tumor Cells but not Chemotherapy Resistant Cells to Anti-UCP Antibody Causes Increased Cell Death

[0278]Chemotherapy-sensitive cells HL60 and chemotherapy resistant cells HL60-MDR were exposed to a labeled anti-UCP antibody (described above) for two 15 minute intervals and subjected to flow cytometry, as described above.

[0279]Scatter plots were generated for each sample. In the first plot the forward versus side scatter of untreated HL60 cells represents a population of healthy living cells. When HL60s were treated with the anti-UCP antibody, the forward scatter decreased and the side scatter increased demonstrating a higher number of dead cells present in the population. It is expected that longer incubation times with the anti-UCP antibody will result in a greater number of dead cells within the population. There was no difference between the anti-UCP untreated and treated HL60-MDR cells. The lack of effect of the UCP antibody on HL60MDR cells was...

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Abstract

The invention is based in part on the discovery that uncoupling proteins (UCPs) are expressed in the plasma membrane of rapidly dividing cells but not of growth arrested, chemotherapy resistant cells. It has also been found according to the invention that UCP is expressed in the lysosomal membrane under certain metabolic conditions. Thus the invention is methods, products, screening assays and kits relating to the manipulation of UCP expression within cellular and intracellular membranes.

Description

RELATED APPLICATIONS[0001]This application is a continuation of U.S. application Ser. No. 11 / 409,446, entitled “METHODS AND PRODUCTS FOR MANIPULATING UNCOUPLING PROTEIN EXPRESSION” filed on Apr. 21, 2006, which is herein incorporated by reference in its entirety. Application Ser. No. 11 / 409,446 is a continuation of U.S. application Ser. No. 09 / 599,760, entitled “METHODS AND PRODUCTS FOR MANIPULATING UNCOUPLING PROTEIN EXPRESSION” filed on Jun. 22, 2000, which is herein incorporated by reference in its entirety. application Ser. No. 09 / 599,760 claims priority under 35 U.S.C. §119(e) to U.S. Provisional Application Ser. No. 60 / 140,574, entitled “METHODS AND PRODUCTS FOR MANIPULATING UNCOUPLING PROTEIN EXPRESSION IN THE PLASMA MEMBRANE” filed on Jun. 23, 1998, which is herein incorporated by reference in its entirety.FIELD OF THE INVENTION[0002]The present invention relates to the discovery that the cellular location of uncoupling protein (UCP) is altered in some cell types under diffe...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): A61K9/127C12N5/00A61K39/12A61K39/02A61K39/00A61P37/00A61P31/04A61P31/10A61P31/12A61P33/00A61P35/00A61K39/395A61K38/17A61K9/10G01N33/48A61K31/19A61K31/7052A61K31/7068A61K31/7072A61K31/7076A61K31/708A61K38/00A61K39/002A61K45/00A61K47/42A61K47/48A61K48/00A61P3/10A61P17/00A61P29/00A61P35/02A61P37/02A61P43/00C07K14/47C07K16/18C12N5/07C12N5/077C12N5/0781C12N5/0783C12N5/0787C12N5/09C12N15/09C12Q1/02C12Q1/68G01N33/50
CPCA61K38/00A61K39/395A61K2039/505C07K14/47C07K16/18G01N2333/47G01N33/5011A61K2300/00A61P17/00A61P29/00A61P31/00A61P31/04A61P31/10A61P31/12A61P33/00A61P35/00A61P35/02A61P37/00A61P37/02A61P43/00A61P3/10Y02A50/30
Inventor NEWELL, MARTHA K.
Owner UNIVERSITY OF VERMONT
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