334 results about "Forward scatter" patented technology

A method for identifying, analyzing, and quantifying the cellular components of whole blood by means of an automated hematology analyzer and the detection of the light scattered, absorbed, and fluorescently emitted by each cell. More particularly, the aforementioned method involves identifying, analyzing, and quantifying the cellular components of whole blood by means of a light source having a wavelength ranging from about 400 nm to about 450 nm and multiple in-flow optical measurements and staining without the need for lysing red blood cells.

A charged particle beam exposure method is disclosed, which includes preparing an aperture mask having character apertures, correcting dimensions of designed patterns in design data in consideration of at least one of factors such as a forward scattering distance of a charged particle, a rearward scattering distance of the charged particle, a blurring of a beam of the charged particle, a dimension conversion difference of the designed patterns due to a denseness/coarseness difference of the designed patterns caused when the underlayer is processed while using the resist as a mask, and the like, allocating at least a part of a specified character aperture of the plurality of character apertures of the aperture mask to the corrected designed patterns to produce writing data, and exposing the resist to the beams of the charged particle passed through the at least a part of the specified character aperture based on the writing data.

The system includes an excitation source for providing a beam of electromagnetic radiation having a source wavelength. A first wavelength selective device is positioned to be impinged by the beam of electromagnetic radiation. The first wavelength selective device is constructed to transmit at least a portion of any radiation having the source wavelength and to reflect radiation of other wavelengths. A medium containing particles is positioned to be impinged by the beam of electromagnetic radiation. At least a portion of the beam of electromagnetic radiation becomes scattered within the medium, the scattered electromagnetic radiation including forward scattered electromagnetic radiation and backward scattered electromagnetic radiation. An optical detector is positioned to receive backward and/or forward scattered electromagnetic radiation.

Blood cells of interest are readily distinguishable from other blood cells and look-a-like particles found in a blood sample by their back-scatter signature. A preferred method for differentiating platelets in a blood sample is to irradiate the cells and particles, one at a time, with a beam of radiation, and to detect back-scattered (reflected) radiation using a plurality of optical fibers to transmit the back-scattered radiation to a high-gain photodetector, e.g. a photomultiplier tube. Preferably, the back-scatter signal so obtained is combined with a second signal representing, for example, either the level of forward-scatter within a prescribed, relatively narrow angular range, or the level of side-scattered radiation, or the level of attenuation of the cell-irradiating beam caused by the presence of the irradiated cell or particle in the beam, or the electrical impedance of the irradiated cell or particle, to differentiate the cells of interest. The method and apparatus of the invention are particularly useful in differentiating platelets and basophils in a blood sample.

The present invention is a flow cytometry-based hematology system useful in the analysis of biological samples, particularly whole blood or blood-derived samples. The system is capable of determining at least a complete blood count (CBC), a five-part white blood cell differential, and a reticulocyte count from a whole blood sample. The system preferably uses a laser diode that emits a thin beam to illuminate cells in a flow cell and a lensless optical detection system to measure one or more of axial light loss, low-angle forward scattered light, high-angle forward scattered light, right angle scattered light, and time-of-flight measurements produced by the cells. The lensless optical detection system contains no optical components, other than photoreactive elements, and does not include any moving parts. Finally, the system uses a unique system of consumable reagent tubes that act as reaction chambers, mixing chambers, and waste chambers for the blood sample analyses. The consumable tubes incorporate reference particles, which act as internal standards to ensure that the dilutions made during processing of the samples have been carried out correctly, and to ensure that the instrument is working properly. The present invention also relates to methods for using the system.

The labyrinth includes probing chamber composed of labyrinth cover and labyrinth base, as well as forward scattering light path and back scattering light path constituted from transmitting tube (3), transmitting tube (6) and receiving tube. Dual scattering light paths including two transmitting paths and one receiving path are adopted in the invention. Using characteristics of different responses on particles in different colors from forward or back scatterings, the method recognizes smog in different colors and grain sizes in order to distinguish not fire alarm factors such as water spray, dust etc so as to raise responsibility of detector, and lower rate of false alarm. Comparing prior art, the invention possesses advantages of superior orientation responsibility, reasonable design, balanced responses for smog in different colors and grain sizes, and lower cost. The invention is applicable to spot type optical detector of fire smoke in auto fire alarm system.

The invention provides a measuring method which can classify and count myeloblast more precisely without influence of other component in a sample including platelet aggregation in measurement of a blood sample with a flowcytometry, wherein damage is given to a cell membrane of erythrocyte and mature leukocyte contained in a hematological sample, a hemocyte in which a cell membrane is damaged is constricted, and this is dyeing-treated with a fluorescent dye which can stain a nucleic acid to obtain a sample, the sample is measured with a flowcytometer, and a cell contained in a first cell group containing myeloblast, which is specified based on forward scattered light information and side scattered light information, and contained in a second cell group containing myeloblast, which is specified based on forward scattered light information and fluorescent information, is counted as myeloblast. The invention further provides a measuring device comprising following systems: a sample treating system, an information acquisition system, a first specific system, a second specific system and a counting system.

In an optical scanning system for detecting particles and pattern defects on a sample surface, a light beam is focused to an illuminated spot on the surface and the spot is scanned across the surface along a scan line. A detector is positioned adjacent to the surface to collect scattered light from the spot where the detector includes a one- or two-dimensional array of sensors. Light scattered from the illuminated spot at each of a plurality of positions along the scan line is focused onto a corresponding sensor in the array. A plurality of detectors symmetrically placed with respect to the illuminating beam detect laterally and forward scattered light from the spot. The spot is scanned over arrays of scan line segments shorter than the dimensions of the surface. A bright field channel enables the adjustment of the height of the sample surface to correct for errors caused by height variations of the surface. Different defect maps provided by the output of the detectors can be compared to identify and classify the defects. The imaging function of the array of sensors combines the advantages of a scanning system and an imaging system while improving signal/background ratio of the system.

An array of multiple light-emitting diodes each having a different wavelength are equally spaced around a tubular sample holder facing inwardly. A photo-diode light sensor that detects light transmitted through the sample is mounted to the tubular sample holder at a position directly opposite each light-emitting diode. By selectively turning on one of the LED's and selecting the associated photo-diode exactly opposite it, the light transmission through a solution can be ascertained. A computer is then used to plot the transmission and absorption on a graph for each of the different wavelengths. The circular array of sensors and LED's results in one sensor directly opposite each LED, one sensor off-axis on either side in the forward scatter direction, and one sensor off-axis on either side in the back scatter direction. The combination of back-scattered light and forward-scattered light and transmitted light permits measurements of the turbidity of the sample solution.

The invention discloses a forward scattering optical signal detecting device used by a flow cell analyzer or a particle analyzer, a forward scattering optical signal detecting method and a cell or particle analyzer provided with the device. The forward scattering optical signal detecting device comprises a detecting unit and a collecting unit, wherein the detecting unit is provided with a plurality of subsidiary detecting units arranged in a one-dimensional position and used for detecting the forward scattering optical signals; the collecting unit used for collecting forward scattering optical signals and focusing the forward scattering optical signals on the detecting unit; and the optical signals detected by the subsidiary detecting units in different positions or intervals are used as the optical signals with different scattering angles. The invention can reduce the system cost and keep the miniaturized advantage, is easy to debug and can feely select the collected optical signals with different scattering angles so as to more precisely distinguish all subgroups in cells or particles to be detected.

The invention discloses a flow cytometry. The flow cytometry comprises a light source irradiation system, a liquid flow system, a light split system, a signal detection, analysis and processing system, and a personal computer (PC) display control system; the liquid flow system comprises a flowing chamber; linearly flowing cells are accommodated in the flowing chamber; a solid laser is used as an excitation light source of the light source irradiation system, and laser passes through a reflecting mirror and a plano-convex lens to be converged and irradiated on the flowing chamber, is integrated and then is further converged on the optical part of the flowing chamber to be irradiated on the linearly flowing cells; the signal detection, analysis and processing system comprises a lateral scattering detector, a yellow fluorescence channel detector, a near-infrared fluorescence channel detector, a forward scattering detector and a signal processing module; and all detectors are multi-pixel photon counters (MPPC). The flow cytometry has the characteristics of large dynamic detection range, high sensitivity, small size and compactness and convenience in maintenance.

A method for formulating an exposure dose for an electron beam on a resist film for a pattern of geometric shapes which compensates for electron scattering effects utilizing hierarchial design data which is preserved to as great as an extent as possible in the computation of the exposure dose. The exposure dose is corrected for both the forward scatter and backscatter effects of the electron beam in which the design data is modified for interactions of shapes which are affected only over the forward scatter range. In another version of the method, a multiple Gaussian approximation is used where the short term Gaussian terms are treated as the forward scatter terms and the long term Gaussian terms are treated as the back scatter terms.

A laser light source emits a first light beam irradiating a solution including target particles and being flowed in a flow cell to generate forward scattered light and orthogonal scattered light therefrom. A light emitting diode emits a second light beam irradiating the solution in the flow cell to generate at least one wavelength of fluorescence therefrom. A first detector is adapted to detect the forward scattered light. A second detector is adapted to detect the orthogonal scattered light. At least one third detector is adapted to detect the at least one fluorescence. A first filter is disposed between the flow cell and the third detector and adapted to eliminate scattered light generated from the target particles by the irradiation of the first light beam.

A shaped illumination panel having a broad-area sheet-form configuration and including a flexible sheet of an optically transmissive material and one or more light sources optically coupled to an edge of the flexible sheet. The flexible sheet may include forward-deflecting or forward-scattering elements configured to extract light from one location of the flexible sheet and direct the extracted light to a second location of the flexible sheet where the extracted light can be transversely propagated through and reemitted from the flexible sheet.

A system and method for identifying organisms by analysis of scattergrams of colonies is disclosed. cattergrams are obtained by culturing samples and illuminating the resultant colonies by a laser. The forward scattered light is imaged and subject to a feature extraction process. The feature vector may include Zernike or Chebyshev moments and may also include Harelick texture features. Feature vectors may be used to train a classification process using either supervised or unsupervised machine learning techniques. The classification process may be used to associate a colony phenotype with the genotype of the sample.

System and method for detecting and counting bacteria suspended in a biological fluid by means of light scattering measurements is provided. In accordance with the method of the invention the level of signal to noise of the measured intensities of light scattered by a sample of the biological fluid is significantly enhanced for forwardly scattered light within a range of scattering angles which are smaller compared to a predefined maximal scattering angle. The system of the invention includes a cuvette adapted to contain a sample of the biological fluid whose sidewalls and windows are suitably constructed and arranged to significantly reduce the level of reflected light obscuring the scattering patterns measured within the range of scattering angles considered.

In an embodiment of the invention, a method of measuring bone density is provided comprising: irradiating a volume to be analyzed; for x-rays emerging from the irradiated volume, detecting at least two of transmitted x-rays, forward scattered x-rays and backward scattered x-rays; and analyzing at least two of the transmitted x-rays, the forward scattered x-rays, or the backward scattered x-rays to determine a density by determining at least one of a ratio of the forward scattered x-rays to the backward scattered x-rays, a ratio of the forward scattered x-rays to the transmitted x-rays, or the product of the ratio of the forward scattered x-rays to the backward scattered x-rays and the ratio of the forward scattered x-rays to the transmitted x-rays.

A particle analyzing apparatus, comprising: a flow cell which forms a specimen flow including particles; first and second light sources; an irradiation optical system which applies lights emitted from the first and second light sources so that the lights are applied to the specimen flow; a detector which detects forward scattered light, the forward scattered light being emitted from the first light and scattered by the particle in the specimen flow, and generates a signal according to the detected scattered light; a light blocking member disposed between the flow cell and the detector; a controller which obtains characteristic parameters of the particle based on the signal from the detector; and an imaging device which captures an image of the particle in the specimen flow using the light from the second light source is disclosed. Particle imaging method is also disclosed.

Spatial filtering is disclosed that improves the signal to noise ration of a sample inspection system of the type having a detector and collection optics that receive radiation scattered from a point on a sample surface and direct the scattered radiation toward the detector. The spatial filtering may screen the detector from substantially all of the forward-scattered radiation from back-scattered radiation that is scattered in a at an elevation angle less than about 45° with respect to a normal to the surface. Forward scattered noise is screened from the detector while backscattered signal reaches the detector. Programmable spatial filters may be used to selectively block scattered noise due to surface roughness while transmitting scattered signal due to surface defects.