This invention proposes a method for rapid determination of the content of
Phellodendron amurense
charcoal slices processed at different temperatures using near-
infrared spectroscopy. Near-
infrared spectroscopy is used to collect spectral information of
Phellodendron amurense
charcoal slices processed at different temperatures. Background interference is eliminated through preprocessing methods such as baseline correction, standard normal variable transformation, multivariate
scattering correction, and first derivative. Combined with competitive adaptive reweighted sampling, CARS combined with iterative retention of information variables, and iterative combination optimization, a
partial least squares regression quantitative prediction model is established. This model achieves accurate prediction of the content of five key components: 5-O-FQA, 3-O-FQA,
berberine,
berberine, and
berberine, with prediction correlation coefficients all exceeding 0.90. This invention combines spectral information with chemometric methods to establish a
digital content prediction model based on near-
infrared spectroscopy for online monitoring of the
Phellodendron amurense
charcoal slice
processing process. It has the advantages of objectivity, speed, non-destructive nature, and reliability, effectively solving the technical problem of lack of objectivity in existing technologies based on
human judgment.